Hprt1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hprt1
Entrez Gene ID	24465
Full Name	hypoxanthine phosphoribosyltransferase 1
Synonyms	Hgprtase,Hprt
General protein information	Preferred Names hypoxanthine phosphoribosyltransferase 1 Names hypoxanthine-guanine phosphoribosyltransferase HGPRT hypoxanthine guanine phosphoribosyl transferase 1 hypoxanthine phosphoribosyl transferase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq37
Summary	catalyzes the conversion of IMP and diphosphate to hypoxanthine and 5-phospho-alpha-D-ribose 1-diphosphate [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012583.2	NP_036715.1	hypoxanthine-guanine phosphoribosyltransferase

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	hprt1	NP_989312.1
	LOC709186	XP_001097691.1
Canis lupus familiaris (dog)	HPRT1	NP_001003357.1
Bos taurus (cattle)	LOC510369	XP_002686486.1
Mus musculus (house mouse)	Hprt	NP_038584.2
Gallus gallus (chicken)	HPRT1	NP_990179.1
Caenorhabditis elegans (roundworm)	CELE_Y105E8B.5	NP_493545.1
Homo sapiens (human)	HPRT1	NP_000185.1
Pan troglodytes (chimpanzee)	HPRT1	NP_001104287.1
Rattus norvegicus (Norway rat)	Hprt1	NP_036715.1
Danio rerio (zebrafish)	hprt1	NP_998151.1

Gene Ontology
Bibliography

Related articles in PubMed

Genotoxicity of phenacetin in the kidney and liver of Sprague-Dawley gpt delta transgenic rats in 26-week and 52-week repeated-dose studies.
Kawamura Y, Hayashi H, Masumura K, Numazawa S, Nohmi T
Toxicology32410-7(2014 Oct)

Validation of four reference genes for quantitative mRNA expression studies in a rat model of inflammatory injury.
Walder RY, Wattiez AS, White SR, Marquez de Prado B, Hamity MV, Hammond DL
Molecular pain1055(2014 Sep)

Hypoxanthine guanine phosphoribosyltransferase (HPRT) deficiencies: HPRT1 mutations in new Japanese families and PRPP concentration.
Yamada Y, Nomura N, Yamada K, Kimura R, Fukushi D, Wakamatsu N, Matsuda Y, Yamauchi T, Ueda T, Hasegawa H, Nakamura M, Ichida K, Kaneko K, Fujimori S
Nucleosides, nucleotides & nucleic acids33(4-6)218-22(2014)

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W
Immunology and cell biology88(8)851-6(2010 Nov-Dec)

Analysis of the HPRT1 gene in 35 Italian Lesch-Nyhan families: 45 patients and 77 potential female carriers.
de Gemmis P, Anesi L, Lorenzetto E, Gioachini I, Fortunati E, Zandon? G, Fanin E, Fairbanks L, Andrighetto G, Parmigiani P, Dolcetta D, Nyhan WL, Hladnik U
Mutation research692(1-2)1-5(2010 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Report frequency of phenacetin induced gtp point mutations in the liver and kidney of gpt transgenic rats.
Title: Genotoxicity of phenacetin in the kidney and liver of Sprague-Dawley gpt delta transgenic rats in 26-week and 52-week repeated-dose studies.

Thsi study validated the Hprt1, Actb, Mapk6 or B2m and showed that any one or combination of two of them are good reference genes for normalization of mRNA expression in qPCR experiments in the spinal cord and RVM in the CFA model of inflammatory injury.
Title: Validation of four reference genes for quantitative mRNA expression studies in a rat model of inflammatory injury.

Mutations in the HPRT gene were evaluated in female Big Blue rats and found not to be induced by leucomalachite green administered in a chronic feeding study.
Title: Analysis of mutations and bone marrow micronuclei in Big Blue rats fed leucomalachite green.

The following Hprt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hprt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00390	NM_012583.2 Latest version!	Rattus norvegicus hypoxanthine phosphoribosyltransferase 1 (Hprt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hprt1 ( NM_012583.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036715.1 Rattus norvegicus hypoxanthine phosphoribosyltransferase 1 (Hprt1), mRNA.

Price & Availability↑

CloneID	ORa00390
Clone ID Related Accession (Same CDS sequence)	NM_012583.2
Accession Version	NM_012583.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Rattus norvegicus(Norway rat)
Product	hypoxanthine-guanine phosphoribosyltransferase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC098629.1. On or before Dec 15, 2013 this sequence version replaced XM_003752155.2, NM_012583.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC098629.1, X62085.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTCGACCC TCAGTCCCAG CGTCGTGATT AGTGATGATG AACCAGGTTA TGACCTAGAT 
TTATTTTGCA TACCTAATCA TTATGCTGAA GATTTGGAAA AGGTGTTTAT TCCTCATGGA 
CTGATTATGG ACAGGACTGA AAGACTTGCT CGAGATGTCA TGAAGGAGAT GGGAGGCCAT 
CACATTGTGG CCCTCTGTGT GCTGAAGGGG GGCTATAAGT TCTTTGCTGA CCTGCTGGAT 
TACATTAAAG CGCTGAATAG AAATAGTGAT AGGTCCATTC CTATGACTGT AGATTTTATC 
AGACTGAAGA GCTACTGTAA TGACCAGTCA ACGGGGGACA TAAAAGTTAT TGGTGGAGAT 
GATCTCTCAA CTTTAACTGG AAAGAACGTC TTGATTGTTG AAGATATAAT TGACACTGGT 
AAAACAATGC AGACTTTGCT TTCCTTGGTC AAGCAGTACA GCCCCAAAAT GGTTAAGGTT 
GCAAGCTTGC TGGTGAAAAG GACCTCTCGA AGTGTTGGAT ACAGGCCAGA CTTTGTTGGA 
TTTGAAATTC CAGACAAGTT TGTTGTTGGA TATGCCCTTG ACTATAATGA GCACTTCAGG 
GATTTGAATC ATGTTTGTGT CATCAGCGAA AGTGGAAAAG CCAAGTACAA AGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036715.1
CDS	67..723
Translation	MSTLSPSVVISDDEPGYDLDLFCIPNHYAEDLEKVFIPHGLIMDRTERLARDVMKEMGGHHIVALCVLKGGYKFFADLLDYIKALNRNSDRSIPMTVDFIRLKSYCNDQSTGDIKVIGGDDLSTLTGKNVLIVEDIIDTGKTMQTLLSLVKQYSPKMVKVASLLVKRTSRSVGYRPDFVGFEIPDKFVVGYALDYNEHFRDLNHVCVISESGKAKYKA

Target ORF information:

RefSeq Version	NM_012583.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hypoxanthine phosphoribosyltransferase 1 (Hprt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012583.2

ORF Insert Sequence:

ATGTCGACCC TCAGTCCCAG CGTCGTGATT AGTGATGATG AACCAGGTTA TGACCTAGAT 
TTATTTTGCA TACCTAATCA TTATGCTGAA GATTTGGAAA AGGTGTTTAT TCCTCATGGA 
CTGATTATGG ACAGGACTGA AAGACTTGCT CGAGATGTCA TGAAGGAGAT GGGAGGCCAT 
CACATTGTGG CCCTCTGTGT GCTGAAGGGG GGCTATAAGT TCTTTGCTGA CCTGCTGGAT 
TACATTAAAG CGCTGAATAG AAATAGTGAT AGGTCCATTC CTATGACTGT AGATTTTATC 
AGACTGAAGA GCTACTGTAA TGACCAGTCA ACGGGGGACA TAAAAGTTAT TGGTGGAGAT 
GATCTCTCAA CTTTAACTGG AAAGAACGTC TTGATTGTTG AAGATATAAT TGACACTGGT 
AAAACAATGC AGACTTTGCT TTCCTTGGTC AAGCAGTACA GCCCCAAAAT GGTTAAGGTT 
GCAAGCTTGC TGGTGAAAAG GACCTCTCGA AGTGTTGGAT ACAGGCCAGA CTTTGTTGGA 
TTTGAAATTC CAGACAAGTT TGTTGTTGGA TATGCCCTTG ACTATAATGA GCACTTCAGG 
GATTTGAATC ATGTTTGTGT CATCAGCGAA AGTGGAAAAG CCAAGTACAA AGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genotoxicity of phenacetin in the kidney and liver of Sprague-Dawley gpt delta transgenic rats in 26-week and 52-week repeated-dose studies.
Toxicology32410-7(2014 Oct)
Kawamura Y,Hayashi H,Masumura K,Numazawa S,Nohmi T

Validation of four reference genes for quantitative mRNA expression studies in a rat model of inflammatory injury.
Molecular pain1055(2014 Sep)
Walder RY,Wattiez AS,White SR,Marquez de Prado B,Hamity MV,Hammond DL

Hypoxanthine guanine phosphoribosyltransferase (HPRT) deficiencies: HPRT1 mutations in new Japanese families and PRPP concentration.
Nucleosides, nucleotides & nucleic acids33(4-6)218-22(2014)
Yamada Y,Nomura N,Yamada K,Kimura R,Fukushi D,Wakamatsu N,Matsuda Y,Yamauchi T,Ueda T,Hasegawa H,Nakamura M,Ichida K,Kaneko K,Fujimori S

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Immunology and cell biology88(8)851-6(2010 Nov-Dec)
Buschow SI,van Balkom BW,Aalberts M,Heck AJ,Wauben M,Stoorvogel W

Analysis of the HPRT1 gene in 35 Italian Lesch-Nyhan families: 45 patients and 77 potential female carriers.
Mutation research692(1-2)1-5(2010 Oct)
de Gemmis P,Anesi L,Lorenzetto E,Gioachini I,Fortunati E,Zandon? G,Fanin E,Fairbanks L,Andrighetto G,Parmigiani P,Dolcetta D,Nyhan WL,Hladnik U

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Hprt1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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