H1f6 cDNA ORF clone, Rattus norvegicus(Norway rat)

Proteomic characterization of the human sperm nucleus.
de Mateo S, Castillo J, Estanyol JM, Ballesc? JL, Oliva R
Proteomics11(13)2714-26(2011 Jul)

Testis-specific linker histone H1t is multiply phosphorylated during spermatogenesis. Identification of phosphorylation sites.
Sarg B, Chwatal S, Talasz H, Lindner HH
The Journal of biological chemistry284(6)3610-8(2009 Feb)

Binding of RFX2 and NF-Y to the testis-specific histone H1t promoter may be required for transcriptional activation in primary spermatocytes.
VanWert JM, Wolfe SA, Grimes SR
Journal of cellular biochemistry104(3)1087-101(2008 Jun)

Meiotic failure in male mice lacking an X-linked factor.
Yang F, Gell K, van der Heijden GW, Eckardt S, Leu NA, Page DC, Benavente R, Her C, H??g C, McLaughlin KJ, Wang PJ
Genes & development22(5)682-91(2008 Mar)

Mouse TEX15 is essential for DNA double-strand break repair and chromosomal synapsis during male meiosis.
Yang F, Eckardt S, Leu NA, McLaughlin KJ, Wang PJ
The Journal of cell biology180(4)673-9(2008 Feb)

it appears that not the order of up-phosphorylation but the number of the phosphate groups is necessary for regulated chromatin decondensation, thus facilitating the substitution of H1t by transition proteins and protamines.
Title: Testis-specific linker histone H1t is multiply phosphorylated during spermatogenesis. Identification of phosphorylation sites.

RFX2 and NF-Y binding to the testis-specific histone H1t promoter may be required for transcriptional activation in primary spermatocytes
Title: Binding of RFX2 and NF-Y to the testis-specific histone H1t promoter may be required for transcriptional activation in primary spermatocytes.

The glutamine residue in histone H1t forms a hydrogen bond with main chain carbonyl of methionine-52 (in H1t) and is spatially oriented away from the nucleosome dyad axis.
Title: A K52Q substitution in the globular domain of histone H1t modulates its nucleosome binding properties.

These data support the hypothesis that RFX2 expression is upregulated in spermatocytes where it participates in activating transcription of the H1t gene and other testis genes.
Title: Transcription factor RFX2 is abundant in rat testis and enriched in nuclei of primary spermatocytes where it appears to be required for transcription of the testis-specific histone H1t gene.

RFX2 is strongly expressed in pachytene spermatocytes, remains high in early round spermatids, and declines only in advance of nuclear condensation. Maximum expression correlates well with the appearance of H1t.
Title: RFX2 is a potential transcriptional regulatory factor for histone H1t and other genes expressed during the meiotic phase of spermatogenesis.