Grin2d cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Grin2d
Entrez Gene ID	24412
Full Name	glutamate ionotropic receptor NMDA type subunit 2D
Synonyms	GluN2D
General protein information	Preferred Names glutamate ionotropic receptor NMDA type subunit 2D Names glutamate receptor ionotropic, NMDA 2D N-methyl D-aspartate receptor subtype 2D NMDAR2D NR2D glutamate [NMDA] receptor subunit epsilon-4 glutamate receptor, ionotropic, N-methyl D-aspartate 2D glutamate receptor, ionotropic, NMDA2D
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q22
Summary	N-methyl-D-aspartate receptor subunit; expressed in mid-brain structures with peak expression at day P7 [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008759316.2	XP_008757538.1	glutamate receptor ionotropic, NMDA 2D isoform X1
XM_008759317.2	XP_008757539.1	glutamate receptor ionotropic, NMDA 2D isoform X2
NM_022797.1	NP_073634.1	glutamate receptor ionotropic, NMDA 2D precursor

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012429	XP_314428.4
Xenopus tropicalis (tropical clawed frog)	grin2d	XP_002935032.1
Homo sapiens (human)	GRIN2D	NP_000827.2
Drosophila melanogaster (fruit fly)	Nmdar2	NP_001162637.1
Canis lupus familiaris (dog)	GRIN2D	XP_005616409.1
Mus musculus (house mouse)	Grin2d	NP_032198.2
Rattus norvegicus (Norway rat)	Grin2d	NP_073634.1
Bos taurus (cattle)	GRIN2D	XP_002695241.2
Caenorhabditis elegans (roundworm)	nmr-2	NP_001256640.1

Gene Ontology
Bibliography

Related articles in PubMed

GRIN2D Recurrent De Novo Dominant Mutation Causes a Severe Epileptic Encephalopathy Treatable with NMDA Receptor Channel Blockers.
Li D, Yuan H, Ortiz-Gonzalez XR, Marsh ED, Tian L, McCormick EM, Kosobucki GJ, Chen W, Schulien AJ, Chiavacci R, Tankovic A, Naase C, Brueckner F, von St?lpnagel-Steinbeis C, Hu C, Kusumoto H, Hedrich UB, Elsen G, H?rtnagel K, Aizenman E, Lemke JR, Hakonarson H, Traynelis SF, Falk MJ
American journal of human genetics99(4)802-816(2016 Oct)

Positive Allosteric Modulators of GluN2A-Containing NMDARs with Distinct Modes of Action and Impacts on Circuit Function.
Hackos DH, Lupardus PJ, Grand T, Chen Y, Wang TM, Reynen P, Gustafson A, Wallweber HJ, Volgraf M, Sellers BD, Schwarz JB, Paoletti P, Sheng M, Zhou Q, Hanson JE
Neuron89(5)983-99(2016 Mar)

Discovery of GluN2A-Selective NMDA Receptor Positive Allosteric Modulators (PAMs): Tuning Deactivation Kinetics via Structure-Based Design.
Volgraf M, Sellers BD, Jiang Y, Wu G, Ly CQ, Villemure E, Pastor RM, Yuen PW, Lu A, Luo X, Liu M, Zhang S, Sun L, Fu Y, Lupardus PJ, Wallweber HJ, Liederer BM, Deshmukh G, Plise E, Tay S, Reynen P, Herrington J, Gustafson A, Liu Y, Dirksen A, Dietz MG, Liu Y, Wang TM, Hanson JE, Hackos D, Scearce-Levie K, Schwarz JB
Journal of medicinal chemistry59(6)2760-79(2016 Mar)

Synthesis and structure activity relationship of tetrahydroisoquinoline-based potentiators of GluN2C and GluN2D containing N-methyl-D-aspartate receptors.
Santangelo Freel RM, Ogden KK, Strong KL, Khatri A, Chepiga KM, Jensen HS, Traynelis SF, Liotta DC
Journal of medicinal chemistry56(13)5351-81(2013 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data from molecular modeling suggest that GluN2D-containing NMDA receptors can enter a conformation or mode that allows the pore to gate with high probability of burst of activity.
Title: Modal gating of GluN1/GluN2D NMDA receptors.

Data indicate that tetrahydroisoquinolines showed subunit-selective potentiation of NMDA receptors containing the GluN2C or GluN2D subunits.
Title: Synthesis and structure activity relationship of tetrahydroisoquinoline-based potentiators of GluN2C and GluN2D containing N-methyl-D-aspartate receptors.

NR1, NR2B, and NR2D were robustly expressed in the supraoptic nucleus of adult rats.
Title: NMDA receptor subunit expression in the supraoptic nucleus of adult rats: dominance of NR2B and NR2D.

Expression of NR2D mRNAs in the thalamus was increased by mustard oil application to molar tooth pulp. This was prevented by lidocaine & reversed by MK-801. Naloxone antagonized this reversal.
Title: The role of N-methyl-D-aspartate receptor subunits in the rat thalamic mediodorsal nucleus during central sensitization.

The deactivation time of GluN1/GluN2D receptors is influenced by the conformational variability of the ligand-binding domain and the structure of the ligand.
Title: Ligand-specific deactivation time course of GluN1/GluN2D NMDA receptors.

The following Grin2d gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Grin2d cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12364	NM_022797.1 Latest version!	Rattus norvegicus glutamate ionotropic receptor NMDA type subunit 2D (Grin2d), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
ORa42908	XM_008759316.2 Latest version!	Rattus norvegicus glutamate ionotropic receptor NMDA type subunit 2D (Grin2d), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
ORa42909	XM_008759317.2 Latest version!	Rattus norvegicus glutamate ionotropic receptor NMDA type subunit 2D (Grin2d), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Grin2d ( NM_022797.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_073634.1 Rattus norvegicus glutamate ionotropic receptor NMDA type subunit 2D (Grin2d), mRNA.

Price & Availability↑

CloneID	ORa12364
Clone ID Related Accession (Same CDS sequence)	NM_022797.1
Accession Version	NM_022797.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	glutamate receptor ionotropic, NMDA 2D precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L31612.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L31612.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCGCGGCG CCGGTGGCCC CCGCGGCCCT CGGGGCCCCG CTAAGATGCT GCTGCTGCTG 
GCGCTGGCGT GCGCCAGCCC GTTCCCGGAG GAGGTGCCGG GGCCGGGCGC GGTCGGCGGG 
GGCACGGGCG GGGCGCGGCC ACTCAACGTG GCGCTTGTCT TCTCCGGCCC GGCATATGCG 
GCAGAGGCGG CGCGCTTGGG CCCGGCCGTG GCAGCGGCAG TGCGCAGCCC CGGCCTGGAC 
GTACGGCCCG TGGCGCTGGT GCTCAACGGC TCCGACCCTC CGAGCCTTGT GCTGCAGCTC 
TGCGACCTGC TGTCGGGGCT GCGCGTGCAC GGCGTGGTGT TCGAGGACGA CTCGCGCGCG 
CCCGCCGTCG CGCCCATTCT CGACTTCCTG TCCGCGCAGA CCTCGCTGCC CATCGTAGCC 
GTGCACGGCG GCGCCGCGCT CGTGCTCACA CCCAAGGAGA AGGGCTCCAC CTTCCTGCAG 
CTTGGCTCCT CCACAGAGCA ACAGTTGCAG GTCATTTTTG AGGTGCTGGA GGAGTACGAC 
TGGACATCCT TTGTGGCAGT GACCACGCGT GCCCCAGGCC ATCGGGCCTT CTTGTCATAC 
ATCGAGGTGC TGACTGATGG CAGCCTGGTG GGCTGGGAGC ATCGAGGAGC GCTGACACTG 
GACCCCGGGG CGGGTGAGGC TGTACTGGGC GCACAGCTCC GTAGTGTCAG TGCGCAGATC 
CGCCTGCTCT TCTGCGCCCG CGAGGAGGCA GAGCCTGTTT TCCGGGCAGC GGAAGAGGCC 
GGCCTCACTG GGCCTGGCTA CGTCTGGTTC ATGGTGGGAC CCCAGCTGGC TGGAGGCGGG 
GGCTCCGGGG TCCCTGGGGA GCCCCTTCTT CTGCCAGGAG GTTCCCCACT GCCTGCCGGG 
CTGTTTGCAG TGGCCTCTGC TGGCTGGCGT GACGACTTGG CACGTCGAGT GGCTGCTGGT 
GTGGCGGTGG TGGCCAGAGG TGCCCAGGCC CTGCTGCGAG ACTATGGCTT CCTGCCTGAG 
CTGGGTCATG ACTGCCGCAC CCAGAATCGC ACCCACCGCG GGGAGAGTCT GCACAGGTAC 
TTCATGAACA TCACCTGGGA TAACCGAGAC TACTCCTTCA ATGAGGATGG CTTTCTGGTG 
AACCCTTCAC TGGTGGTCAT CTCCCTCACC AGAGACAGGA CATGGGAAGT GGTGGGCAGC 
TGGGAACAGC AGACCCTCCG CCTCAAGTAC CCTCTATGGT CCCGCTATGG CCGCTTCCTG 
CAGCCAGTGG ATGACACACA GCACCTCACT GTGGCCACGC TGGAGGAGAG ACCCTTTGTC 
ATTGTAGAGC CTGCAGACCC CATCAGCGGC ACTTGCATCA GAGACTCGGT CCCCTGCCGG 
AGCCAGCTCA ACCGTACCCA CAGCCCTCCG CCTGACGCTC CCCGCCCGGA GAAGAGATGC 
TGCAAGGGTT TCTGCATTGA TATTCTGAAG AGGCTGGCGC ACACCATCGG TTTCAGCTAT 
GACCTCTACC TGGTTACCAA CGGCAAGCAT GGCAAGAAGA TCGATGGCGT CTGGAATGGC 
ATGATTGGTG AGGTGTTCTA TCAGCGTGCG GACATGGCCA TCGGCTCTCT CACCATCAAT 
GAGGAGCGGT CAGAGATCGT GGACTTCTCC GTCCCCTTTG TAGAGACAGG CATCAGCGTC 
ATGGTGGCAC GCAGCAATGG CACTGTGTCC CCCTCTGCCT TCCTCGAGCC CTACAGCCCT 
GCTGTCTGGG TGATGATGTT CGTCATGTGC CTCACCGTGG TCGCCGTCAC AGTTTTCATC 
TTTGAGTACC TCAGTCCTGT GGGCTATAAC CGAAGCCTGG CCACGGGCAA ACGCCCTGGT 
GGCTCTACCT TCACCATTGG GAAATCCATC TGGCTGCTGT GGGCCCTGGT CTTCAACAAC 
TCCGTGCCAG TGGAGAATCC TCGGGGCACC ACCAGCAAGA TCATGGTGCT GGTGTGGGCC 
TTCTTCGCGG TCATCTTTCT TGCCAGCTAC ACAGCCAATC TGGCCGCCTT CATGATCCAG 
GAGGAGTACG TGGACACCGT GTCTGGGCTC AGCGACCGGA AGTTCCAGCG ACCCCAGGAG 
CAATACCCAC CTCTGAAGTT TGGCACGGTG CCCAATGGGT CCACGGAAAA GAACATCCGG 
AGCAACTACC CTGACATGCA CAGCTACATG GTGCGATACA ACCAGCCGAG AGTGGAGGAG 
GCACTCACAC AGCTCAAGGC AGGGAAACTG GACGCCTTCA TCTATGACGC AGCAGTGCTC 
AACTACATGG CCCGCAAGGA TGAGGGCTGC AAGCTGGTCA CCATCGGCTC CGGCAAGGTC 
TTCGCCACCA CTGGTTATGG CATCGCCCTA CACAAGGGCT CCCGCTGGAA GAGGCCCATC 
GACCTGGCGC TGCTGCAGTT CCTGGGGGAC GATGAGATTG AGATGCTGGA GCGGCTGTGG 
CTTTCAGGGA TCTGCCACAA TGACAAAATC GAAGTGATGA GTAGCAAGCT GGATATCGAC 
AACATGGCAG GTGTCTTCTA CATGCTCCTT GTGGCCATGG GCCTCTCCTT GCTGGTCTTC 
GCCTGGGAAC ATCTGGTGTA CTGGCGCCTG CGGCATTGCC TGGGTCCCAC CCACCGCATG 
GATTTCCTAC TGGCCTTCTC CAGGGGTATG TACAGCTGCT GCAGCGCCGA GGCTGCTCCA 
CCGCCGGCCA AGCCTCCGCC ACCGCCTCAG CCGCTGCCCA GTCCCGCATA TCCCGCCGCT 
CGCCCGCCCC CTGGCCCCGC ACCCTTCGTG CCCCGAGAGC GCGCAGCTGC CGACCGCTGG 
CGCCGAGCCA AGGGCACAGG GCCCCCGGGG GGCGCAGCGA TAGCCGACGG CTTCCACCGA 
TACTACGGCC CCATCGAGCC ACAGGGGCTG GGCCTCGGCG AGGCACGCGC GGCACCGAGA 
GGCGCAGCCG GACGCCCACT GTCCCCACCC ACCACACAGC CCCCGCAGAA GCCACCACCT 
TCCTACTTCG CCATCGTGCG CGAGCAGGAG CCGACCGAGC CCCCGGCCGG CGCCTTCCCC 
GGCTTCCCAT CTCCGCCTGC GCCGCCCGCT GCGGCTGCCG CTGCCGTCGG GCCGCCACTG 
TGTCGCCTGG CTTTCGAGGA CGAAAGCCCG CCCGCACCCT CGCGCTGGCC GCGTTCAGAT 
CCCGAGAGCC AGCCTCTGTT GGGTGGGGGC GCGGGCGGCC CGAGCGCGGG GGCCCCAACC 
GCACCACCGC CGCGCCGTGC CGCGCCACCA CCGTGCGCCT ACCTGGACCT CGAGCCTTCG 
CCTTCGGACT CCGAGGACTC GGAGAGCCTG GGCGGAGCAT CGCTCGGTGG CCTGGAGCCC 
TGGTGGTTCG CCGACTTCCC CTACCCGTAT GCCGAGCGCC TCGGGCCGCC GCCCGGCCGC 
TACTGGTCGG TCGACAAGCT CGGGGGCTGG CGCGCTGGTA GCTGGGACTA CCTGCCCCCG 
CGCGGAGGCC CCGCGTGGCA CTGCCGCCAC TGCGCCAGCC TGGAGCTGCT ACCGCCACCG 
CGCCACCTCA GCTGCTCGCA CGACGGCTTA GACGGCGGCT GGTGGGCGCC TCCGCCTCCA 
CCCTGGGCCG CGGGGCCACC TCCCCGGCGC CGGGCGCGCT GTGGTTGTCC GCGACCTCAC 
CCACACCGCC CCCGGGCCTC GCACCGTGCG CCCGCCGCCG CGCCGCACCA CCACCGACAC 
AGGCGCGCGG CCGGTGGCTG GGACTTCCCG CCGCCCGCGC CCACCTCGCG TTCGCTGGAG 
GACCTGAGCT CCTGCCCGCG GGCGGCCCCC ACGCGCAGGC TCACCGGGCC ATCCCGCCAC 
GCGCGCCGCT GCCCGCACGC TGCGCACTGG GGGCCGCCCC TGCCCACCGC ATCGCACCGG 
AGACACCGGG GCGGGGACCT GGGCACACGC AGGGGCTCTG CGCATTTCTC CAGCCTGGAG 
TCCGAGGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_073634.1
CDS	740..4711
Translation	MRGAGGPRGPRGPAKMLLLLALACASPFPEEVPGPGAVGGGTGGARPLNVALVFSGPAYAAEAARLGPAVAAAVRSPGLDVRPVALVLNGSDPPSLVLQLCDLLSGLRVHGVVFEDDSRAPAVAPILDFLSAQTSLPIVAVHGGAALVLTPKEKGSTFLQLGSSTEQQLQVIFEVLEEYDWTSFVAVTTRAPGHRAFLSYIEVLTDGSLVGWEHRGALTLDPGAGEAVLGAQLRSVSAQIRLLFCAREEAEPVFRAAEEAGLTGPGYVWFMVGPQLAGGGGSGVPGEPLLLPGGSPLPAGLFAVASAGWRDDLARRVAAGVAVVARGAQALLRDYGFLPELGHDCRTQNRTHRGESLHRYFMNITWDNRDYSFNEDGFLVNPSLVVISLTRDRTWEVVGSWEQQTLRLKYPLWSRYGRFLQPVDDTQHLTVATLEERPFVIVEPADPISGTCIRDSVPCRSQLNRTHSPPPDAPRPEKRCCKGFCIDILKRLAHTIGFSYDLYLVTNGKHGKKIDGVWNGMIGEVFYQRADMAIGSLTINEERSEIVDFSVPFVETGISVMVARSNGTVSPSAFLEPYSPAVWVMMFVMCLTVVAVTVFIFEYLSPVGYNRSLATGKRPGGSTFTIGKSIWLLWALVFNNSVPVENPRGTTSKIMVLVWAFFAVIFLASYTANLAAFMIQEEYVDTVSGLSDRKFQRPQEQYPPLKFGTVPNGSTEKNIRSNYPDMHSYMVRYNQPRVEEALTQLKAGKLDAFIYDAAVLNYMARKDEGCKLVTIGSGKVFATTGYGIALHKGSRWKRPIDLALLQFLGDDEIEMLERLWLSGICHNDKIEVMSSKLDIDNMAGVFYMLLVAMGLSLLVFAWEHLVYWRLRHCLGPTHRMDFLLAFSRGMYSCCSAEAAPPPAKPPPPPQPLPSPAYPAARPPPGPAPFVPRERAAADRWRRAKGTGPPGGAAIADGFHRYYGPIEPQGLGLGEARAAPRGAAGRPLSPPTTQPPQKPPPSYFAIVREQEPTEPPAGAFPGFPSPPAPPAAAAAAVGPPLCRLAFEDESPPAPSRWPRSDPESQPLLGGGAGGPSAGAPTAPPPRRAAPPPCAYLDLEPSPSDSEDSESLGGASLGGLEPWWFADFPYPYAERLGPPPGRYWSVDKLGGWRAGSWDYLPPRGGPAWHCRHCASLELLPPPRHLSCSHDGLDGGWWAPPPPPWAAGPPPRRRARCGCPRPHPHRPRASHRAPAAAPHHHRHRRAAGGWDFPPPAPTSRSLEDLSSCPRAAPTRRLTGPSRHARRCPHAAHWGPPLPTASHRRHRGGDLGTRRGSAHFSSLESEV

Target ORF information:

RefSeq Version	NM_022797.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutamate ionotropic receptor NMDA type subunit 2D (Grin2d), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022797.1

ORF Insert Sequence:

ATGCGCGGCG CCGGTGGCCC CCGCGGCCCT CGGGGCCCCG CTAAGATGCT GCTGCTGCTG 
GCGCTGGCGT GCGCCAGCCC GTTCCCGGAG GAGGTGCCGG GGCCGGGCGC GGTCGGCGGG 
GGCACGGGCG GGGCGCGGCC ACTCAACGTG GCGCTTGTCT TCTCCGGCCC GGCATATGCG 
GCAGAGGCGG CGCGCTTGGG CCCGGCCGTG GCAGCGGCAG TGCGCAGCCC CGGCCTGGAC 
GTACGGCCCG TGGCGCTGGT GCTCAACGGC TCCGACCCTC CGAGCCTTGT GCTGCAGCTC 
TGCGACCTGC TGTCGGGGCT GCGCGTGCAC GGCGTGGTGT TCGAGGACGA CTCGCGCGCG 
CCCGCCGTCG CGCCCATTCT CGACTTCCTG TCCGCGCAGA CCTCGCTGCC CATCGTAGCC 
GTGCACGGCG GCGCCGCGCT CGTGCTCACA CCCAAGGAGA AGGGCTCCAC CTTCCTGCAG 
CTTGGCTCCT CCACAGAGCA ACAGTTGCAG GTCATTTTTG AGGTGCTGGA GGAGTACGAC 
TGGACATCCT TTGTGGCAGT GACCACGCGT GCCCCAGGCC ATCGGGCCTT CTTGTCATAC 
ATCGAGGTGC TGACTGATGG CAGCCTGGTG GGCTGGGAGC ATCGAGGAGC GCTGACACTG 
GACCCCGGGG CGGGTGAGGC TGTACTGGGC GCACAGCTCC GTAGTGTCAG TGCGCAGATC 
CGCCTGCTCT TCTGCGCCCG CGAGGAGGCA GAGCCTGTTT TCCGGGCAGC GGAAGAGGCC 
GGCCTCACTG GGCCTGGCTA CGTCTGGTTC ATGGTGGGAC CCCAGCTGGC TGGAGGCGGG 
GGCTCCGGGG TCCCTGGGGA GCCCCTTCTT CTGCCAGGAG GTTCCCCACT GCCTGCCGGG 
CTGTTTGCAG TGGCCTCTGC TGGCTGGCGT GACGACTTGG CACGTCGAGT GGCTGCTGGT 
GTGGCGGTGG TGGCCAGAGG TGCCCAGGCC CTGCTGCGAG ACTATGGCTT CCTGCCTGAG 
CTGGGTCATG ACTGCCGCAC CCAGAATCGC ACCCACCGCG GGGAGAGTCT GCACAGGTAC 
TTCATGAACA TCACCTGGGA TAACCGAGAC TACTCCTTCA ATGAGGATGG CTTTCTGGTG 
AACCCTTCAC TGGTGGTCAT CTCCCTCACC AGAGACAGGA CATGGGAAGT GGTGGGCAGC 
TGGGAACAGC AGACCCTCCG CCTCAAGTAC CCTCTATGGT CCCGCTATGG CCGCTTCCTG 
CAGCCAGTGG ATGACACACA GCACCTCACT GTGGCCACGC TGGAGGAGAG ACCCTTTGTC 
ATTGTAGAGC CTGCAGACCC CATCAGCGGC ACTTGCATCA GAGACTCGGT CCCCTGCCGG 
AGCCAGCTCA ACCGTACCCA CAGCCCTCCG CCTGACGCTC CCCGCCCGGA GAAGAGATGC 
TGCAAGGGTT TCTGCATTGA TATTCTGAAG AGGCTGGCGC ACACCATCGG TTTCAGCTAT 
GACCTCTACC TGGTTACCAA CGGCAAGCAT GGCAAGAAGA TCGATGGCGT CTGGAATGGC 
ATGATTGGTG AGGTGTTCTA TCAGCGTGCG GACATGGCCA TCGGCTCTCT CACCATCAAT 
GAGGAGCGGT CAGAGATCGT GGACTTCTCC GTCCCCTTTG TAGAGACAGG CATCAGCGTC 
ATGGTGGCAC GCAGCAATGG CACTGTGTCC CCCTCTGCCT TCCTCGAGCC CTACAGCCCT 
GCTGTCTGGG TGATGATGTT CGTCATGTGC CTCACCGTGG TCGCCGTCAC AGTTTTCATC 
TTTGAGTACC TCAGTCCTGT GGGCTATAAC CGAAGCCTGG CCACGGGCAA ACGCCCTGGT 
GGCTCTACCT TCACCATTGG GAAATCCATC TGGCTGCTGT GGGCCCTGGT CTTCAACAAC 
TCCGTGCCAG TGGAGAATCC TCGGGGCACC ACCAGCAAGA TCATGGTGCT GGTGTGGGCC 
TTCTTCGCGG TCATCTTTCT TGCCAGCTAC ACAGCCAATC TGGCCGCCTT CATGATCCAG 
GAGGAGTACG TGGACACCGT GTCTGGGCTC AGCGACCGGA AGTTCCAGCG ACCCCAGGAG 
CAATACCCAC CTCTGAAGTT TGGCACGGTG CCCAATGGGT CCACGGAAAA GAACATCCGG 
AGCAACTACC CTGACATGCA CAGCTACATG GTGCGATACA ACCAGCCGAG AGTGGAGGAG 
GCACTCACAC AGCTCAAGGC AGGGAAACTG GACGCCTTCA TCTATGACGC AGCAGTGCTC 
AACTACATGG CCCGCAAGGA TGAGGGCTGC AAGCTGGTCA CCATCGGCTC CGGCAAGGTC 
TTCGCCACCA CTGGTTATGG CATCGCCCTA CACAAGGGCT CCCGCTGGAA GAGGCCCATC 
GACCTGGCGC TGCTGCAGTT CCTGGGGGAC GATGAGATTG AGATGCTGGA GCGGCTGTGG 
CTTTCAGGGA TCTGCCACAA TGACAAAATC GAAGTGATGA GTAGCAAGCT GGATATCGAC 
AACATGGCAG GTGTCTTCTA CATGCTCCTT GTGGCCATGG GCCTCTCCTT GCTGGTCTTC 
GCCTGGGAAC ATCTGGTGTA CTGGCGCCTG CGGCATTGCC TGGGTCCCAC CCACCGCATG 
GATTTCCTAC TGGCCTTCTC CAGGGGTATG TACAGCTGCT GCAGCGCCGA GGCTGCTCCA 
CCGCCGGCCA AGCCTCCGCC ACCGCCTCAG CCGCTGCCCA GTCCCGCATA TCCCGCCGCT 
CGCCCGCCCC CTGGCCCCGC ACCCTTCGTG CCCCGAGAGC GCGCAGCTGC CGACCGCTGG 
CGCCGAGCCA AGGGCACAGG GCCCCCGGGG GGCGCAGCGA TAGCCGACGG CTTCCACCGA 
TACTACGGCC CCATCGAGCC ACAGGGGCTG GGCCTCGGCG AGGCACGCGC GGCACCGAGA 
GGCGCAGCCG GACGCCCACT GTCCCCACCC ACCACACAGC CCCCGCAGAA GCCACCACCT 
TCCTACTTCG CCATCGTGCG CGAGCAGGAG CCGACCGAGC CCCCGGCCGG CGCCTTCCCC 
GGCTTCCCAT CTCCGCCTGC GCCGCCCGCT GCGGCTGCCG CTGCCGTCGG GCCGCCACTG 
TGTCGCCTGG CTTTCGAGGA CGAAAGCCCG CCCGCACCCT CGCGCTGGCC GCGTTCAGAT 
CCCGAGAGCC AGCCTCTGTT GGGTGGGGGC GCGGGCGGCC CGAGCGCGGG GGCCCCAACC 
GCACCACCGC CGCGCCGTGC CGCGCCACCA CCGTGCGCCT ACCTGGACCT CGAGCCTTCG 
CCTTCGGACT CCGAGGACTC GGAGAGCCTG GGCGGAGCAT CGCTCGGTGG CCTGGAGCCC 
TGGTGGTTCG CCGACTTCCC CTACCCGTAT GCCGAGCGCC TCGGGCCGCC GCCCGGCCGC 
TACTGGTCGG TCGACAAGCT CGGGGGCTGG CGCGCTGGTA GCTGGGACTA CCTGCCCCCG 
CGCGGAGGCC CCGCGTGGCA CTGCCGCCAC TGCGCCAGCC TGGAGCTGCT ACCGCCACCG 
CGCCACCTCA GCTGCTCGCA CGACGGCTTA GACGGCGGCT GGTGGGCGCC TCCGCCTCCA 
CCCTGGGCCG CGGGGCCACC TCCCCGGCGC CGGGCGCGCT GTGGTTGTCC GCGACCTCAC 
CCACACCGCC CCCGGGCCTC GCACCGTGCG CCCGCCGCCG CGCCGCACCA CCACCGACAC 
AGGCGCGCGG CCGGTGGCTG GGACTTCCCG CCGCCCGCGC CCACCTCGCG TTCGCTGGAG 
GACCTGAGCT CCTGCCCGCG GGCGGCCCCC ACGCGCAGGC TCACCGGGCC ATCCCGCCAC 
GCGCGCCGCT GCCCGCACGC TGCGCACTGG GGGCCGCCCC TGCCCACCGC ATCGCACCGG 
AGACACCGGG GCGGGGACCT GGGCACACGC AGGGGCTCTG CGCATTTCTC CAGCCTGGAG 
TCCGAGGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grin2d ( XM_008759316.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008757538.1 Rattus norvegicus glutamate ionotropic receptor NMDA type subunit 2D (Grin2d), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa42908
Clone ID Related Accession (Same CDS sequence)	XM_008759316.2
Accession Version	XM_008759316.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	glutamate receptor ionotropic, NMDA 2D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008759316.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGGCG CCGGTGGCCC CCGCGGCCCT CGGGGCCCCG CTAAGATGCT GCTGCTGCTG 
GCGCTGGCGT GCGCCAGCCC GTTCCCGGAG GAGGTGCCGG GGCCGGGCGC GGTCGGCGGG 
GGCACGGGCG GGGCGCGGCC ACTCAACGTG GCGCTTGTCT TCTCCGGCCC GGCATATGCG 
GCAGAGGCGG CGCGCTTGGG CCCGGCCGTG GCAGCGGCAG TGCGCAGCCC CGGCCTGGAC 
GTACGGCCCG TGGCGCTGGT GCTCAACGGC TCCGACCCTC GCAGCCTTGT GCTGCAGCTC 
TGCGACCTGC TGTCGGGGCT GCGCGTGCAC GGCGTGGTGT TCGAGGACGA CTCGCGCGCG 
CCCGCCGTCG CGCCCATTCT CGACTTCCTG TCCGCGCAGA CCTCGCTGCC CATCGTAGCC 
GTGCACGGCG GCGCCGCGCT CGTGCTCACA CCCAAGGAGA AGGGCTCCAC CTTCCTGCAG 
CTTGGCTCCT CCACAGAGCA ACAGTTGCAG GTCATTTTTG AGGTGCTGGA GGAGTACGAC 
TGGACATCCT TTGTGGCAGT GACCACGCGT GCCCCAGGCC ATCGGGCCTT CTTGTCATAC 
ATCGAGGTGC TGACTGATGG CAGCCTGGTG GGCTGGGAGC ATCGAGGAGC GCTGACACTG 
GACCCCGGGG CGGGTGAGGC TGTACTGGGC GCACAGCTCC GTAGTGTCAG TGCGCAGATC 
CGCCTGCTCT TCTGCGCCCG CGAGGAGGCA GAGCCTGTTT TCCGGGCAGC GGAAGAGGCC 
GGCCTCACTG GGCCTGGCTA CGTCTGGTTC ATGGTGGGAC CCCAGCTGGC TGGAGGCGGG 
GGCTCCGGGG TCCCTGGGGA GCCCCTTCTT CTGCCAGGAG GTTCCCCACT GCCTGCCGGG 
CTGTTTGCAG TGCGCTCTGC TGGCTGGCGT GACGACTTGG CACGTCGAGT GGCTGCTGGT 
GTGGCGGTGG TGGCCAGAGG TGCCCAGGCC CTGCTGCGAG ACTATGGCTT CCTGCCTGAG 
CTGGGTCATG ACTGCCGCAC CCAGAATCGC ACCCACCGCG GGGAGAGTCT GCACAGGTAC 
TTCATGAACA TCACCTGGGA TAACCGAGAC TACTCCTTCA ATGAGGATGG CTTTCTGGTG 
AACCCTTCAC TGGTGGTCAT CTCCCTCACC AGAGACAGGA CATGGGAAGT GGTGGGCAGC 
TGGGAACAGC AGACCCTCCG CCTCAAGTAC CCTCTATGGT CCCGCTATGG CCGCTTCCTG 
CAGCCAGTGG ATGACACACA GCACCTCACT GTGGCCACGC TGGAGGAGAG ACCCTTTGTC 
ATTGTAGAGC CTGCAGACCC CATCAGCGGC ACTTGCATCA GAGACTCGGT CCCCTGCCGG 
AGCCAGCTCA ACCGTACCCA CAGCCCTCCG CCTGACGCTC CCCGCCCGGA GAAGAGATGC 
TGCAAGGGTT TCTGCATTGA TATTCTGAAG AGGCTGGCGC ACACCATCGG TTTCAGCTAT 
GACCTCTACC TGGTTACCAA CGGCAAGCAT GGCAAGAAGA TCGATGGCGT CTGGAATGGC 
ATGATTGGTG AGGTGTTCTA TCAGCGTGCG GACATGGCCA TCGGCTCTCT CACCATCAAT 
GAGGAGCGGT CAGAGATCGT GGACTTCTCC GTCCCCTTTG TAGAGACAGG CATCAGCGTC 
ATGGTGGCAC GCAGCAATGG CACTGTGTCC CCCTCTGCCT TCCTCGAGCC CTACAGCCCT 
GCTGTCTGGG TGATGATGTT CGTCATGTGC CTCACCGTGG TCGCCGTCAC AGTTTTCATC 
TTTGAGTACC TCAGTCCTGT GGGCTATAAC CGAAGCCTGG CCACGGGCAA ACGCCCTGGT 
GGCTCTACCT TCACCATTGG GAAATCCATC TGGCTGCTGT GGGCCCTGGT CTTCAACAAC 
TCCGTGCCAG TGGAGAATCC TCGGGGCACC ACCAGCAAGA TCATGGTGCT GGTGTGGGCC 
TTCTTCGCGG TCATCTTTCT TGCCAGCTAC ACAGCCAATC TGGCCGCCTT CATGATCCAG 
GAGGAGTACG TGGACACCGT GTCTGGGCTC AGCGACCGGA AGTTCCAGCG ACCCCAGGAG 
CAATACCCAC CTCTGAAGTT TGGCACGGTG CCCAATGGGT CCACGGAAAA GAACATCCGG 
AGCAACTACC CTGACATGCA CAGCTACATG GTGCGATACA ACCAGCCGAG AGTGGAGGAG 
GCACTCACAC AGCTCAAGGC AGGGAAACTG GACGCCTTCA TCTATGACGC AGCAGTGCTC 
AACTACATGG CCCGCAAGGA TGAGGGCTGC AAGCTGGTCA CCATCGGCTC CGGCAAGGTC 
TTCGCCACCA CTGGTTATGG CATCGCCCTA CACAAGGGCT CCCGCTGGAA GAGGCCCATC 
GACCTGGCGC TGCTGCAGTT CCTGGGGGAC GATGAGATTG AGATGCTGGA GCGGCTGTGG 
CTTTCAGGGA TCTGCCACAA TGACAAAATC GAAGTGATGA GTAGCAAGCT GGATATCGAC 
AACATGGCAG GTGTCTTCTA CATGCTCCTT GTGGCCATGG GCCTCTCCTT GCTGGTCTTC 
GCCTGGGAAC ATCTGGTGTA CTGGCGCCTG CGGCATTGCC TGGGTCCCAC CCACCGCATG 
GATTTCCTAC TGGCCTTCTC CAGGGGTATG TACAGCTGCT GCAGCGCCGA GGCTGCTCCA 
CCGCCGGCCA AGCCTCCGCC ACCGCCTCAG CCGCTGCCCA GTCCCGCATA TCCCGCCGCT 
CGCCCGCCCC CTGGCCCCGC ACCCTTCGTG CCCCGAGAGC GCGCAGCTGC CGACCGCTGG 
CGCCGAGCCA AGGGCACAGG GCCCCCGGGG GGCGCAGCGA TAGCCGACGG CTTCCACCGA 
TACTACGGCC CCATCGAGCC ACAGGGGCTG GGCCTCGGCG AGGCACGCGC GGCACCGAGA 
GGCGCAGCCG GACGCCCACT GTCCCCACCC ACCACACAGC CCCCGCAGAA GCCACCACCT 
TCCTACTTCG CCATCGTGCG CGAGCAGGAG CCGACCGAGC CCCCGGCCGG CGCCTTCCCC 
GGCTTCCCAT CTCCGCCTGC GCCGCCCGCT GCGGCTGCCG CTGCCGTCGG GCCGCCACTG 
TGTCGCCTGG CTTTCGAGGA CGAAAGCCCG CCCGCACCCT CGCGCTGGCC GCGTTCAGAT 
CCCGAGAGCC AGCCTCTGTT GGGTGGGGGC GCGGGCGGCC CGAGCGCGGG GGCCCCAACC 
GCACCACCGC CGCGCCGTGC CGCGCCACCA CCGTGCGCCT ACCTGGACCT CGAGCCTTCG 
CCTTCGGACT CCGAGGACTC GGAGAGCCTG GGCGGAGCAT CGCTCGGTGG CCTGGAGCCC 
TGGTGGTTCG CCGACTTCCC CTACCCGTAT GCCGAGCGCC TCGGGCCGCC GCCCGGCCGC 
TACTGGTCGG TCGACAAGCT CGGGGGCTGG CGCGCTGGTA GCTGGGACTA CCTGCCCCCG 
CGCGGAGGCC CCGCGTGGCA CTGCCGCCAC TGCGCCAGCC TGGAGCTGCT ACCGCCACCG 
CGCCACCTCA GCTGCTCGCA CGACGGCTTA GACGGCGGCT GGTGGGCGCC TCCGCCTCCA 
CCCTGGGCCG CGGGGCCACC TCCCCGGCGC CGGGCGCGCT GTGGTTGTCC GCGACCTCAC 
CCACACCGCC CCCGGGCCTC GCACCGTGCG CCCGCCGCCG CGCCGCACCA CCACCGACAC 
AGGCGCGCGG CCGGTGGCTG GGACTTCCCG CCGCCCGCGC CCACCTCGCG TTCGCTGGAG 
GACCTGAGCT CCTGCCCGCG GGCGGCCCCC ACGCGCAGGC TCACCGGGCC ATCCCGCCAC 
GCGCGCCGCT GCCCGCACGC TGCGCACTGG GGGCCGCCCC TGCCCACCGC ATCGCACCGG 
AGACACCGGG GCGGGGACCT GGGCACACGC AGGGGCTCTG CGCATTTCTC CAGCCTGGAG 
TCCGAGGTTT CCAAGGCAAC AGTACAGTGG GACGGGTCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008757538.1
CDS	3009..7010
Translation	MRGAGGPRGPRGPAKMLLLLALACASPFPEEVPGPGAVGGGTGGARPLNVALVFSGPAYAAEAARLGPAVAAAVRSPGLDVRPVALVLNGSDPRSLVLQLCDLLSGLRVHGVVFEDDSRAPAVAPILDFLSAQTSLPIVAVHGGAALVLTPKEKGSTFLQLGSSTEQQLQVIFEVLEEYDWTSFVAVTTRAPGHRAFLSYIEVLTDGSLVGWEHRGALTLDPGAGEAVLGAQLRSVSAQIRLLFCAREEAEPVFRAAEEAGLTGPGYVWFMVGPQLAGGGGSGVPGEPLLLPGGSPLPAGLFAVRSAGWRDDLARRVAAGVAVVARGAQALLRDYGFLPELGHDCRTQNRTHRGESLHRYFMNITWDNRDYSFNEDGFLVNPSLVVISLTRDRTWEVVGSWEQQTLRLKYPLWSRYGRFLQPVDDTQHLTVATLEERPFVIVEPADPISGTCIRDSVPCRSQLNRTHSPPPDAPRPEKRCCKGFCIDILKRLAHTIGFSYDLYLVTNGKHGKKIDGVWNGMIGEVFYQRADMAIGSLTINEERSEIVDFSVPFVETGISVMVARSNGTVSPSAFLEPYSPAVWVMMFVMCLTVVAVTVFIFEYLSPVGYNRSLATGKRPGGSTFTIGKSIWLLWALVFNNSVPVENPRGTTSKIMVLVWAFFAVIFLASYTANLAAFMIQEEYVDTVSGLSDRKFQRPQEQYPPLKFGTVPNGSTEKNIRSNYPDMHSYMVRYNQPRVEEALTQLKAGKLDAFIYDAAVLNYMARKDEGCKLVTIGSGKVFATTGYGIALHKGSRWKRPIDLALLQFLGDDEIEMLERLWLSGICHNDKIEVMSSKLDIDNMAGVFYMLLVAMGLSLLVFAWEHLVYWRLRHCLGPTHRMDFLLAFSRGMYSCCSAEAAPPPAKPPPPPQPLPSPAYPAARPPPGPAPFVPRERAAADRWRRAKGTGPPGGAAIADGFHRYYGPIEPQGLGLGEARAAPRGAAGRPLSPPTTQPPQKPPPSYFAIVREQEPTEPPAGAFPGFPSPPAPPAAAAAAVGPPLCRLAFEDESPPAPSRWPRSDPESQPLLGGGAGGPSAGAPTAPPPRRAAPPPCAYLDLEPSPSDSEDSESLGGASLGGLEPWWFADFPYPYAERLGPPPGRYWSVDKLGGWRAGSWDYLPPRGGPAWHCRHCASLELLPPPRHLSCSHDGLDGGWWAPPPPPWAAGPPPRRRARCGCPRPHPHRPRASHRAPAAAPHHHRHRRAAGGWDFPPPAPTSRSLEDLSSCPRAAPTRRLTGPSRHARRCPHAAHWGPPLPTASHRRHRGGDLGTRRGSAHFSSLESEVSKATVQWDGS

Target ORF information:

RefSeq Version	XM_008759316.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutamate ionotropic receptor NMDA type subunit 2D (Grin2d), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008759316.2

ORF Insert Sequence:

ATGCGCGGCG CCGGTGGCCC CCGCGGCCCT CGGGGCCCCG CTAAGATGCT GCTGCTGCTG 
GCGCTGGCGT GCGCCAGCCC GTTCCCGGAG GAGGTGCCGG GGCCGGGCGC GGTCGGCGGG 
GGCACGGGCG GGGCGCGGCC ACTCAACGTG GCGCTTGTCT TCTCCGGCCC GGCATATGCG 
GCAGAGGCGG CGCGCTTGGG CCCGGCCGTG GCAGCGGCAG TGCGCAGCCC CGGCCTGGAC 
GTACGGCCCG TGGCGCTGGT GCTCAACGGC TCCGACCCTC GCAGCCTTGT GCTGCAGCTC 
TGCGACCTGC TGTCGGGGCT GCGCGTGCAC GGCGTGGTGT TCGAGGACGA CTCGCGCGCG 
CCCGCCGTCG CGCCCATTCT CGACTTCCTG TCCGCGCAGA CCTCGCTGCC CATCGTAGCC 
GTGCACGGCG GCGCCGCGCT CGTGCTCACA CCCAAGGAGA AGGGCTCCAC CTTCCTGCAG 
CTTGGCTCCT CCACAGAGCA ACAGTTGCAG GTCATTTTTG AGGTGCTGGA GGAGTACGAC 
TGGACATCCT TTGTGGCAGT GACCACGCGT GCCCCAGGCC ATCGGGCCTT CTTGTCATAC 
ATCGAGGTGC TGACTGATGG CAGCCTGGTG GGCTGGGAGC ATCGAGGAGC GCTGACACTG 
GACCCCGGGG CGGGTGAGGC TGTACTGGGC GCACAGCTCC GTAGTGTCAG TGCGCAGATC 
CGCCTGCTCT TCTGCGCCCG CGAGGAGGCA GAGCCTGTTT TCCGGGCAGC GGAAGAGGCC 
GGCCTCACTG GGCCTGGCTA CGTCTGGTTC ATGGTGGGAC CCCAGCTGGC TGGAGGCGGG 
GGCTCCGGGG TCCCTGGGGA GCCCCTTCTT CTGCCAGGAG GTTCCCCACT GCCTGCCGGG 
CTGTTTGCAG TGCGCTCTGC TGGCTGGCGT GACGACTTGG CACGTCGAGT GGCTGCTGGT 
GTGGCGGTGG TGGCCAGAGG TGCCCAGGCC CTGCTGCGAG ACTATGGCTT CCTGCCTGAG 
CTGGGTCATG ACTGCCGCAC CCAGAATCGC ACCCACCGCG GGGAGAGTCT GCACAGGTAC 
TTCATGAACA TCACCTGGGA TAACCGAGAC TACTCCTTCA ATGAGGATGG CTTTCTGGTG 
AACCCTTCAC TGGTGGTCAT CTCCCTCACC AGAGACAGGA CATGGGAAGT GGTGGGCAGC 
TGGGAACAGC AGACCCTCCG CCTCAAGTAC CCTCTATGGT CCCGCTATGG CCGCTTCCTG 
CAGCCAGTGG ATGACACACA GCACCTCACT GTGGCCACGC TGGAGGAGAG ACCCTTTGTC 
ATTGTAGAGC CTGCAGACCC CATCAGCGGC ACTTGCATCA GAGACTCGGT CCCCTGCCGG 
AGCCAGCTCA ACCGTACCCA CAGCCCTCCG CCTGACGCTC CCCGCCCGGA GAAGAGATGC 
TGCAAGGGTT TCTGCATTGA TATTCTGAAG AGGCTGGCGC ACACCATCGG TTTCAGCTAT 
GACCTCTACC TGGTTACCAA CGGCAAGCAT GGCAAGAAGA TCGATGGCGT CTGGAATGGC 
ATGATTGGTG AGGTGTTCTA TCAGCGTGCG GACATGGCCA TCGGCTCTCT CACCATCAAT 
GAGGAGCGGT CAGAGATCGT GGACTTCTCC GTCCCCTTTG TAGAGACAGG CATCAGCGTC 
ATGGTGGCAC GCAGCAATGG CACTGTGTCC CCCTCTGCCT TCCTCGAGCC CTACAGCCCT 
GCTGTCTGGG TGATGATGTT CGTCATGTGC CTCACCGTGG TCGCCGTCAC AGTTTTCATC 
TTTGAGTACC TCAGTCCTGT GGGCTATAAC CGAAGCCTGG CCACGGGCAA ACGCCCTGGT 
GGCTCTACCT TCACCATTGG GAAATCCATC TGGCTGCTGT GGGCCCTGGT CTTCAACAAC 
TCCGTGCCAG TGGAGAATCC TCGGGGCACC ACCAGCAAGA TCATGGTGCT GGTGTGGGCC 
TTCTTCGCGG TCATCTTTCT TGCCAGCTAC ACAGCCAATC TGGCCGCCTT CATGATCCAG 
GAGGAGTACG TGGACACCGT GTCTGGGCTC AGCGACCGGA AGTTCCAGCG ACCCCAGGAG 
CAATACCCAC CTCTGAAGTT TGGCACGGTG CCCAATGGGT CCACGGAAAA GAACATCCGG 
AGCAACTACC CTGACATGCA CAGCTACATG GTGCGATACA ACCAGCCGAG AGTGGAGGAG 
GCACTCACAC AGCTCAAGGC AGGGAAACTG GACGCCTTCA TCTATGACGC AGCAGTGCTC 
AACTACATGG CCCGCAAGGA TGAGGGCTGC AAGCTGGTCA CCATCGGCTC CGGCAAGGTC 
TTCGCCACCA CTGGTTATGG CATCGCCCTA CACAAGGGCT CCCGCTGGAA GAGGCCCATC 
GACCTGGCGC TGCTGCAGTT CCTGGGGGAC GATGAGATTG AGATGCTGGA GCGGCTGTGG 
CTTTCAGGGA TCTGCCACAA TGACAAAATC GAAGTGATGA GTAGCAAGCT GGATATCGAC 
AACATGGCAG GTGTCTTCTA CATGCTCCTT GTGGCCATGG GCCTCTCCTT GCTGGTCTTC 
GCCTGGGAAC ATCTGGTGTA CTGGCGCCTG CGGCATTGCC TGGGTCCCAC CCACCGCATG 
GATTTCCTAC TGGCCTTCTC CAGGGGTATG TACAGCTGCT GCAGCGCCGA GGCTGCTCCA 
CCGCCGGCCA AGCCTCCGCC ACCGCCTCAG CCGCTGCCCA GTCCCGCATA TCCCGCCGCT 
CGCCCGCCCC CTGGCCCCGC ACCCTTCGTG CCCCGAGAGC GCGCAGCTGC CGACCGCTGG 
CGCCGAGCCA AGGGCACAGG GCCCCCGGGG GGCGCAGCGA TAGCCGACGG CTTCCACCGA 
TACTACGGCC CCATCGAGCC ACAGGGGCTG GGCCTCGGCG AGGCACGCGC GGCACCGAGA 
GGCGCAGCCG GACGCCCACT GTCCCCACCC ACCACACAGC CCCCGCAGAA GCCACCACCT 
TCCTACTTCG CCATCGTGCG CGAGCAGGAG CCGACCGAGC CCCCGGCCGG CGCCTTCCCC 
GGCTTCCCAT CTCCGCCTGC GCCGCCCGCT GCGGCTGCCG CTGCCGTCGG GCCGCCACTG 
TGTCGCCTGG CTTTCGAGGA CGAAAGCCCG CCCGCACCCT CGCGCTGGCC GCGTTCAGAT 
CCCGAGAGCC AGCCTCTGTT GGGTGGGGGC GCGGGCGGCC CGAGCGCGGG GGCCCCAACC 
GCACCACCGC CGCGCCGTGC CGCGCCACCA CCGTGCGCCT ACCTGGACCT CGAGCCTTCG 
CCTTCGGACT CCGAGGACTC GGAGAGCCTG GGCGGAGCAT CGCTCGGTGG CCTGGAGCCC 
TGGTGGTTCG CCGACTTCCC CTACCCGTAT GCCGAGCGCC TCGGGCCGCC GCCCGGCCGC 
TACTGGTCGG TCGACAAGCT CGGGGGCTGG CGCGCTGGTA GCTGGGACTA CCTGCCCCCG 
CGCGGAGGCC CCGCGTGGCA CTGCCGCCAC TGCGCCAGCC TGGAGCTGCT ACCGCCACCG 
CGCCACCTCA GCTGCTCGCA CGACGGCTTA GACGGCGGCT GGTGGGCGCC TCCGCCTCCA 
CCCTGGGCCG CGGGGCCACC TCCCCGGCGC CGGGCGCGCT GTGGTTGTCC GCGACCTCAC 
CCACACCGCC CCCGGGCCTC GCACCGTGCG CCCGCCGCCG CGCCGCACCA CCACCGACAC 
AGGCGCGCGG CCGGTGGCTG GGACTTCCCG CCGCCCGCGC CCACCTCGCG TTCGCTGGAG 
GACCTGAGCT CCTGCCCGCG GGCGGCCCCC ACGCGCAGGC TCACCGGGCC ATCCCGCCAC 
GCGCGCCGCT GCCCGCACGC TGCGCACTGG GGGCCGCCCC TGCCCACCGC ATCGCACCGG 
AGACACCGGG GCGGGGACCT GGGCACACGC AGGGGCTCTG CGCATTTCTC CAGCCTGGAG 
TCCGAGGTTT CCAAGGCAAC AGTACAGTGG GACGGGTCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grin2d ( XM_008759317.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008757539.1 Rattus norvegicus glutamate ionotropic receptor NMDA type subunit 2D (Grin2d), transcript variant X2, mRNA.

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CloneID	ORa42909
Clone ID Related Accession (Same CDS sequence)	XM_008759317.2
Accession Version	XM_008759317.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	glutamate receptor ionotropic, NMDA 2D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008759317.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGGCG CCGGTGGCCC CCGCGGCCCT CGGGGCCCCG CTAAGATGCT GCTGCTGCTG 
GCGCTGGCGT GCGCCAGCCC GTTCCCGGAG GAGGTGCCGG GGCCGGGCGC GGTCGGCGGG 
GGCACGGGCG GGGCGCGGCC ACTCAACGTG GCGCTTGTCT TCTCCGGCCC GGCATATGCG 
GCAGAGGCGG CGCGCTTGGG CCCGGCCGTG GCAGCGGCAG TGCGCAGCCC CGGCCTGGAC 
GTACGGCCCG TGGCGCTGGT GCTCAACGGC TCCGACCCTC GCAGCCTTGT GCTGCAGCTC 
TGCGACCTGC TGTCGGGGCT GCGCGTGCAC GGCGTGGTGT TCGAGGACGA CTCGCGCGCG 
CCCGCCGTCG CGCCCATTCT CGACTTCCTG TCCGCGCAGA CCTCGCTGCC CATCGTAGCC 
GTGCACGGCG GCGCCGCGCT CGTGCTCACA CCCAAGGAGA AGGGCTCCAC CTTCCTGCAG 
CTTGGCTCCT CCACAGAGCA ACAGTTGCAG GTCATTTTTG AGGTGCTGGA GGAGTACGAC 
TGGACATCCT TTGTGGCAGT GACCACGCGT GCCCCAGGCC ATCGGGCCTT CTTGTCATAC 
ATCGAGGTGC TGACTGATGG CAGCCTGGTG GGCTGGGAGC ATCGAGGAGC GCTGACACTG 
GACCCCGGGG CGGGTGAGGC TGTACTGGGC GCACAGCTCC GTAGTGTCAG TGCGCAGATC 
CGCCTGCTCT TCTGCGCCCG CGAGGAGGCA GAGCCTGTTT TCCGGGCAGC GGAAGAGGCC 
GGCCTCACTG GGCCTGGCTA CGTCTGGTTC ATGGTGGGAC CCCAGCTGGC TGGAGGCGGG 
GGCTCCGGGG TCCCTGGGGA GCCCCTTCTT CTGCCAGGAG GTTCCCCACT GCCTGCCGGG 
CTGTTTGCAG TGCGCTCTGC TGGCTGGCGT GACGACTTGG CACGTCGAGT GGCTGCTGGT 
GTGGCGGTGG TGGCCAGAGG TGCCCAGGCC CTGCTGCGAG ACTATGGCTT CCTGCCTGAG 
CTGGGTCATG ACTGCCGCAC CCAGAATCGC ACCCACCGCG GGGAGAGTCT GCACAGGTAC 
TTCATGAACA TCACCTGGGA TAACCGAGAC TACTCCTTCA ATGAGGATGG CTTTCTGGTG 
AACCCTTCAC TGGTGGTCAT CTCCCTCACC AGAGACAGGA CATGGGAAGT GGTGGGCAGC 
TGGGAACAGC AGACCCTCCG CCTCAAGTAC CCTCTATGGT CCCGCTATGG CCGCTTCCTG 
CAGCCAGTGG ATGACACACA GCACCTCACT GTGGCCACGC TGGAGGAGAG ACCCTTTGTC 
ATTGTAGAGC CTGCAGACCC CATCAGCGGC ACTTGCATCA GAGACTCGGT CCCCTGCCGG 
AGCCAGCTCA ACCGTACCCA CAGCCCTCCG CCTGACGCTC CCCGCCCGGA GAAGAGATGC 
TGCAAGGGTT TCTGCATTGA TATTCTGAAG AGGCTGGCGC ACACCATCGG TTTCAGCTAT 
GACCTCTACC TGGTTACCAA CGGCAAGCAT GGCAAGAAGA TCGATGGCGT CTGGAATGGC 
ATGATTGGTG AGGTGTTCTA TCAGCGTGCG GACATGGCCA TCGGCTCTCT CACCATCAAT 
GAGGAGCGGT CAGAGATCGT GGACTTCTCC GTCCCCTTTG TAGAGACAGG CATCAGCGTC 
ATGGTGGCAC GCAGCAATGG CACTGTGTCC CCCTCTGCCT TCCTCGAGCC CTACAGCCCT 
GCTGTCTGGG TGATGATGTT CGTCATGTGC CTCACCGTGG TCGCCGTCAC AGTTTTCATC 
TTTGAGTACC TCAGTCCTGT GGGCTATAAC CGAAGCCTGG CCACGGGCAA ACGCCCTGGT 
GGCTCTACCT TCACCATTGG GAAATCCATC TGGCTGCTGT GGGCCCTGGT CTTCAACAAC 
TCCGTGCCAG TGGAGAATCC TCGGGGCACC ACCAGCAAGA TCATGGTGCT GGTGTGGGCC 
TTCTTCGCGG TCATCTTTCT TGCCAGCTAC ACAGCCAATC TGGCCGCCTT CATGATCCAG 
GAGGAGTACG TGGACACCGT GTCTGGGCTC AGCGACCGGA AGTTCCAGCG ACCCCAGGAG 
CAATACCCAC CTCTGAAGTT TGGCACGGTG CCCAATGGGT CCACGGAAAA GAACATCCGG 
AGCAACTACC CTGACATGCA CAGCTACATG GTGCGATACA ACCAGCCGAG AGTGGAGGAG 
GCACTCACAC AGCTCAAGGC AGGGAAACTG GACGCCTTCA TCTATGACGC AGCAGTGCTC 
AACTACATGG CCCGCAAGGA TGAGGGCTGC AAGCTGGTCA CCATCGGCTC CGGCAAGGTC 
TTCGCCACCA CTGGTTATGG CATCGCCCTA CACAAGGGCT CCCGCTGGAA GAGGCCCATC 
GACCTGGCGC TGCTGCAGTT CCTGGGGGAC GATGAGATTG AGATGCTGGA GCGGCTGTGG 
CTTTCAGGGA TCTGCCACAA TGACAAAATC GAAGTGATGA GTAGCAAGCT GGATATCGAC 
AACATGGCAG GTGTCTTCTA CATGCTCCTT GTGGCCATGG GCCTCTCCTT GCTGGTCTTC 
GCCTGGGAAC ATCTGGTGTA CTGGCGCCTG CGGCATTGCC TGGGTCCCAC CCACCGCATG 
GATTTCCTAC TGGCCTTCTC CAGGGGTATG TACAGCTGCT GCAGCGCCGA GGCTGCTCCA 
CCGCCGGCCA AGCCTCCGCC ACCGCCTCAG CCGCTGCCCA GTCCCGCATA TCCCGCCGCT 
CGCCCGCCCC CTGGCCCCGC ACCCTTCGTG CCCCGAGAGC GCGCAGCTGC CGACCGCTGG 
CGCCGAGCCA AGGGCACAGG GCCCCCGGGG GGCGCAGCGA TAGCCGACGG CTTCCACCGA 
TACTACGGCC CCATCGAGCC ACAGGGGCTG GGCCTCGGCG AGGCACGCGC GGCACCGAGA 
GGCGCAGCCG GACGCCCACT GTCCCCACCC ACCACACAGC CCCCGCAGAA GCCACCACCT 
TCCTACTTCG CCATCGTGCG CGAGCAGGAG CCGACCGAGC CCCCGGCCGG CGCCTTCCCC 
GGCTTCCCAT CTCCGCCTGC GCCGCCCGCT GCGGCTGCCG CTGCCGTCGG GCCGCCACTG 
TGTCGCCTGG CTTTCGAGGA CGAAAGCCCG CCCGCACCCT CGCGCTGGCC GCGTTCAGAT 
CCCGAGAGCC AGCCTCTGTT GGGTGGGGGC GCGGGCGGCC CGAGCGCGGG GGCCCCAACC 
GCACCACCGC CGCGCCGTGC CGCGCCACCA CCGTGCGCCT ACCTGGACCT CGAGCCTTCG 
CCTTCGGACT CCGAGGACTC GGAGAGCCTG GGCGGAGCAT CGCTCGGTGG CCTGGAGCCC 
TGGTGGTTCG CCGACTTCCC CTACCCGTAT GCCGAGCGCC TCGGGCCGCC GCCCGGCCGC 
TACTGGTCGG TCGACAAGCT CGGGGGCTGG CGCGCTGGTA GCTGGGACTA CCTGCCCCCG 
CGCGGAGGCC CCGCGTGGCA CTGCCGCCAC TGCGCCAGCC TGGAGCTGCT ACCGCCACCG 
CGCCACCTCA GCTGCTCGCA CGACGGCTTA GACGGCGGCT GGTGGGCGCC TCCGCCTCCA 
CCCTGGGCCG CGGGGCCACC TCCCCGGCGC CGGGCGCGCT GTGGTTGTCC GCGACCTCAC 
CCACACCGCC CCCGGGCCTC GCACCGTGCG CCCGCCGCCG CGCCGCACCA CCACCGACAC 
AGGCGCGCGG CCGGTGGCTG GGACTTCCCG CCGCCCGCGC CCACCTCGCG TTCGCTGGAG 
GACCTGAGCT CCCGCCCCTG CCCACCGCAT CGCACCGGAG ACACCGGGGC GGGGACCTGG 
GCACACGCAG GGGCTCTGCG CATTTCTCCA GCCTGGAGTC CGAGGTATGA CGCGGCTCCG 
GCCCCCACCC CCACCCCCGC CGCCCCCTCG GTCAGCGCAG GCCACGGCCC GAGGGGGCGC 
GCTAAGTGGA CAGGACCCTC ATGGGTTGGG AAGGACAGAA ACGGACCTGG CCGGACCCCG 
CCTGGAGCAG CGTCCTGCGC CCCCACCCCC TTCGCCTTGG GGGAACTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008757539.1
CDS	6121..10191
Translation	MRGAGGPRGPRGPAKMLLLLALACASPFPEEVPGPGAVGGGTGGARPLNVALVFSGPAYAAEAARLGPAVAAAVRSPGLDVRPVALVLNGSDPRSLVLQLCDLLSGLRVHGVVFEDDSRAPAVAPILDFLSAQTSLPIVAVHGGAALVLTPKEKGSTFLQLGSSTEQQLQVIFEVLEEYDWTSFVAVTTRAPGHRAFLSYIEVLTDGSLVGWEHRGALTLDPGAGEAVLGAQLRSVSAQIRLLFCAREEAEPVFRAAEEAGLTGPGYVWFMVGPQLAGGGGSGVPGEPLLLPGGSPLPAGLFAVRSAGWRDDLARRVAAGVAVVARGAQALLRDYGFLPELGHDCRTQNRTHRGESLHRYFMNITWDNRDYSFNEDGFLVNPSLVVISLTRDRTWEVVGSWEQQTLRLKYPLWSRYGRFLQPVDDTQHLTVATLEERPFVIVEPADPISGTCIRDSVPCRSQLNRTHSPPPDAPRPEKRCCKGFCIDILKRLAHTIGFSYDLYLVTNGKHGKKIDGVWNGMIGEVFYQRADMAIGSLTINEERSEIVDFSVPFVETGISVMVARSNGTVSPSAFLEPYSPAVWVMMFVMCLTVVAVTVFIFEYLSPVGYNRSLATGKRPGGSTFTIGKSIWLLWALVFNNSVPVENPRGTTSKIMVLVWAFFAVIFLASYTANLAAFMIQEEYVDTVSGLSDRKFQRPQEQYPPLKFGTVPNGSTEKNIRSNYPDMHSYMVRYNQPRVEEALTQLKAGKLDAFIYDAAVLNYMARKDEGCKLVTIGSGKVFATTGYGIALHKGSRWKRPIDLALLQFLGDDEIEMLERLWLSGICHNDKIEVMSSKLDIDNMAGVFYMLLVAMGLSLLVFAWEHLVYWRLRHCLGPTHRMDFLLAFSRGMYSCCSAEAAPPPAKPPPPPQPLPSPAYPAARPPPGPAPFVPRERAAADRWRRAKGTGPPGGAAIADGFHRYYGPIEPQGLGLGEARAAPRGAAGRPLSPPTTQPPQKPPPSYFAIVREQEPTEPPAGAFPGFPSPPAPPAAAAAAVGPPLCRLAFEDESPPAPSRWPRSDPESQPLLGGGAGGPSAGAPTAPPPRRAAPPPCAYLDLEPSPSDSEDSESLGGASLGGLEPWWFADFPYPYAERLGPPPGRYWSVDKLGGWRAGSWDYLPPRGGPAWHCRHCASLELLPPPRHLSCSHDGLDGGWWAPPPPPWAAGPPPRRRARCGCPRPHPHRPRASHRAPAAAPHHHRHRRAAGGWDFPPPAPTSRSLEDLSSRPCPPHRTGDTGAGTWAHAGALRISPAWSPRYDAAPAPTPTPAAPSVSAGHGPRGRAKWTGPSWVGKDRNGPGRTPPGAASCAPTPFALGEL

Target ORF information:

RefSeq Version	XM_008759317.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutamate ionotropic receptor NMDA type subunit 2D (Grin2d), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008759317.2

ORF Insert Sequence:

ATGCGCGGCG CCGGTGGCCC CCGCGGCCCT CGGGGCCCCG CTAAGATGCT GCTGCTGCTG 
GCGCTGGCGT GCGCCAGCCC GTTCCCGGAG GAGGTGCCGG GGCCGGGCGC GGTCGGCGGG 
GGCACGGGCG GGGCGCGGCC ACTCAACGTG GCGCTTGTCT TCTCCGGCCC GGCATATGCG 
GCAGAGGCGG CGCGCTTGGG CCCGGCCGTG GCAGCGGCAG TGCGCAGCCC CGGCCTGGAC 
GTACGGCCCG TGGCGCTGGT GCTCAACGGC TCCGACCCTC GCAGCCTTGT GCTGCAGCTC 
TGCGACCTGC TGTCGGGGCT GCGCGTGCAC GGCGTGGTGT TCGAGGACGA CTCGCGCGCG 
CCCGCCGTCG CGCCCATTCT CGACTTCCTG TCCGCGCAGA CCTCGCTGCC CATCGTAGCC 
GTGCACGGCG GCGCCGCGCT CGTGCTCACA CCCAAGGAGA AGGGCTCCAC CTTCCTGCAG 
CTTGGCTCCT CCACAGAGCA ACAGTTGCAG GTCATTTTTG AGGTGCTGGA GGAGTACGAC 
TGGACATCCT TTGTGGCAGT GACCACGCGT GCCCCAGGCC ATCGGGCCTT CTTGTCATAC 
ATCGAGGTGC TGACTGATGG CAGCCTGGTG GGCTGGGAGC ATCGAGGAGC GCTGACACTG 
GACCCCGGGG CGGGTGAGGC TGTACTGGGC GCACAGCTCC GTAGTGTCAG TGCGCAGATC 
CGCCTGCTCT TCTGCGCCCG CGAGGAGGCA GAGCCTGTTT TCCGGGCAGC GGAAGAGGCC 
GGCCTCACTG GGCCTGGCTA CGTCTGGTTC ATGGTGGGAC CCCAGCTGGC TGGAGGCGGG 
GGCTCCGGGG TCCCTGGGGA GCCCCTTCTT CTGCCAGGAG GTTCCCCACT GCCTGCCGGG 
CTGTTTGCAG TGCGCTCTGC TGGCTGGCGT GACGACTTGG CACGTCGAGT GGCTGCTGGT 
GTGGCGGTGG TGGCCAGAGG TGCCCAGGCC CTGCTGCGAG ACTATGGCTT CCTGCCTGAG 
CTGGGTCATG ACTGCCGCAC CCAGAATCGC ACCCACCGCG GGGAGAGTCT GCACAGGTAC 
TTCATGAACA TCACCTGGGA TAACCGAGAC TACTCCTTCA ATGAGGATGG CTTTCTGGTG 
AACCCTTCAC TGGTGGTCAT CTCCCTCACC AGAGACAGGA CATGGGAAGT GGTGGGCAGC 
TGGGAACAGC AGACCCTCCG CCTCAAGTAC CCTCTATGGT CCCGCTATGG CCGCTTCCTG 
CAGCCAGTGG ATGACACACA GCACCTCACT GTGGCCACGC TGGAGGAGAG ACCCTTTGTC 
ATTGTAGAGC CTGCAGACCC CATCAGCGGC ACTTGCATCA GAGACTCGGT CCCCTGCCGG 
AGCCAGCTCA ACCGTACCCA CAGCCCTCCG CCTGACGCTC CCCGCCCGGA GAAGAGATGC 
TGCAAGGGTT TCTGCATTGA TATTCTGAAG AGGCTGGCGC ACACCATCGG TTTCAGCTAT 
GACCTCTACC TGGTTACCAA CGGCAAGCAT GGCAAGAAGA TCGATGGCGT CTGGAATGGC 
ATGATTGGTG AGGTGTTCTA TCAGCGTGCG GACATGGCCA TCGGCTCTCT CACCATCAAT 
GAGGAGCGGT CAGAGATCGT GGACTTCTCC GTCCCCTTTG TAGAGACAGG CATCAGCGTC 
ATGGTGGCAC GCAGCAATGG CACTGTGTCC CCCTCTGCCT TCCTCGAGCC CTACAGCCCT 
GCTGTCTGGG TGATGATGTT CGTCATGTGC CTCACCGTGG TCGCCGTCAC AGTTTTCATC 
TTTGAGTACC TCAGTCCTGT GGGCTATAAC CGAAGCCTGG CCACGGGCAA ACGCCCTGGT 
GGCTCTACCT TCACCATTGG GAAATCCATC TGGCTGCTGT GGGCCCTGGT CTTCAACAAC 
TCCGTGCCAG TGGAGAATCC TCGGGGCACC ACCAGCAAGA TCATGGTGCT GGTGTGGGCC 
TTCTTCGCGG TCATCTTTCT TGCCAGCTAC ACAGCCAATC TGGCCGCCTT CATGATCCAG 
GAGGAGTACG TGGACACCGT GTCTGGGCTC AGCGACCGGA AGTTCCAGCG ACCCCAGGAG 
CAATACCCAC CTCTGAAGTT TGGCACGGTG CCCAATGGGT CCACGGAAAA GAACATCCGG 
AGCAACTACC CTGACATGCA CAGCTACATG GTGCGATACA ACCAGCCGAG AGTGGAGGAG 
GCACTCACAC AGCTCAAGGC AGGGAAACTG GACGCCTTCA TCTATGACGC AGCAGTGCTC 
AACTACATGG CCCGCAAGGA TGAGGGCTGC AAGCTGGTCA CCATCGGCTC CGGCAAGGTC 
TTCGCCACCA CTGGTTATGG CATCGCCCTA CACAAGGGCT CCCGCTGGAA GAGGCCCATC 
GACCTGGCGC TGCTGCAGTT CCTGGGGGAC GATGAGATTG AGATGCTGGA GCGGCTGTGG 
CTTTCAGGGA TCTGCCACAA TGACAAAATC GAAGTGATGA GTAGCAAGCT GGATATCGAC 
AACATGGCAG GTGTCTTCTA CATGCTCCTT GTGGCCATGG GCCTCTCCTT GCTGGTCTTC 
GCCTGGGAAC ATCTGGTGTA CTGGCGCCTG CGGCATTGCC TGGGTCCCAC CCACCGCATG 
GATTTCCTAC TGGCCTTCTC CAGGGGTATG TACAGCTGCT GCAGCGCCGA GGCTGCTCCA 
CCGCCGGCCA AGCCTCCGCC ACCGCCTCAG CCGCTGCCCA GTCCCGCATA TCCCGCCGCT 
CGCCCGCCCC CTGGCCCCGC ACCCTTCGTG CCCCGAGAGC GCGCAGCTGC CGACCGCTGG 
CGCCGAGCCA AGGGCACAGG GCCCCCGGGG GGCGCAGCGA TAGCCGACGG CTTCCACCGA 
TACTACGGCC CCATCGAGCC ACAGGGGCTG GGCCTCGGCG AGGCACGCGC GGCACCGAGA 
GGCGCAGCCG GACGCCCACT GTCCCCACCC ACCACACAGC CCCCGCAGAA GCCACCACCT 
TCCTACTTCG CCATCGTGCG CGAGCAGGAG CCGACCGAGC CCCCGGCCGG CGCCTTCCCC 
GGCTTCCCAT CTCCGCCTGC GCCGCCCGCT GCGGCTGCCG CTGCCGTCGG GCCGCCACTG 
TGTCGCCTGG CTTTCGAGGA CGAAAGCCCG CCCGCACCCT CGCGCTGGCC GCGTTCAGAT 
CCCGAGAGCC AGCCTCTGTT GGGTGGGGGC GCGGGCGGCC CGAGCGCGGG GGCCCCAACC 
GCACCACCGC CGCGCCGTGC CGCGCCACCA CCGTGCGCCT ACCTGGACCT CGAGCCTTCG 
CCTTCGGACT CCGAGGACTC GGAGAGCCTG GGCGGAGCAT CGCTCGGTGG CCTGGAGCCC 
TGGTGGTTCG CCGACTTCCC CTACCCGTAT GCCGAGCGCC TCGGGCCGCC GCCCGGCCGC 
TACTGGTCGG TCGACAAGCT CGGGGGCTGG CGCGCTGGTA GCTGGGACTA CCTGCCCCCG 
CGCGGAGGCC CCGCGTGGCA CTGCCGCCAC TGCGCCAGCC TGGAGCTGCT ACCGCCACCG 
CGCCACCTCA GCTGCTCGCA CGACGGCTTA GACGGCGGCT GGTGGGCGCC TCCGCCTCCA 
CCCTGGGCCG CGGGGCCACC TCCCCGGCGC CGGGCGCGCT GTGGTTGTCC GCGACCTCAC 
CCACACCGCC CCCGGGCCTC GCACCGTGCG CCCGCCGCCG CGCCGCACCA CCACCGACAC 
AGGCGCGCGG CCGGTGGCTG GGACTTCCCG CCGCCCGCGC CCACCTCGCG TTCGCTGGAG 
GACCTGAGCT CCCGCCCCTG CCCACCGCAT CGCACCGGAG ACACCGGGGC GGGGACCTGG 
GCACACGCAG GGGCTCTGCG CATTTCTCCA GCCTGGAGTC CGAGGTATGA CGCGGCTCCG 
GCCCCCACCC CCACCCCCGC CGCCCCCTCG GTCAGCGCAG GCCACGGCCC GAGGGGGCGC 
GCTAAGTGGA CAGGACCCTC ATGGGTTGGG AAGGACAGAA ACGGACCTGG CCGGACCCCG 
CCTGGAGCAG CGTCCTGCGC CCCCACCCCC TTCGCCTTGG GGGAACTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

GRIN2D Recurrent De Novo Dominant Mutation Causes a Severe Epileptic Encephalopathy Treatable with NMDA Receptor Channel Blockers.
American journal of human genetics99(4)802-816(2016 Oct)
Li D,Yuan H,Ortiz-Gonzalez XR,Marsh ED,Tian L,McCormick EM,Kosobucki GJ,Chen W,Schulien AJ,Chiavacci R,Tankovic A,Naase C,Brueckner F,von St?lpnagel-Steinbeis C,Hu C,Kusumoto H,Hedrich UB,Elsen G,H?rtnagel K,Aizenman E,Lemke JR,Hakonarson H,Traynelis SF,Falk MJ

Positive Allosteric Modulators of GluN2A-Containing NMDARs with Distinct Modes of Action and Impacts on Circuit Function.
Neuron89(5)983-99(2016 Mar)
Hackos DH,Lupardus PJ,Grand T,Chen Y,Wang TM,Reynen P,Gustafson A,Wallweber HJ,Volgraf M,Sellers BD,Schwarz JB,Paoletti P,Sheng M,Zhou Q,Hanson JE

Discovery of GluN2A-Selective NMDA Receptor Positive Allosteric Modulators (PAMs): Tuning Deactivation Kinetics via Structure-Based Design.
Journal of medicinal chemistry59(6)2760-79(2016 Mar)
Volgraf M,Sellers BD,Jiang Y,Wu G,Ly CQ,Villemure E,Pastor RM,Yuen PW,Lu A,Luo X,Liu M,Zhang S,Sun L,Fu Y,Lupardus PJ,Wallweber HJ,Liederer BM,Deshmukh G,Plise E,Tay S,Reynen P,Herrington J,Gustafson A,Liu Y,Dirksen A,Dietz MG,Liu Y,Wang TM,Hanson JE,Hackos D,Scearce-Levie K,Schwarz JB

Synthesis and structure activity relationship of tetrahydroisoquinoline-based potentiators of GluN2C and GluN2D containing N-methyl-D-aspartate receptors.
Journal of medicinal chemistry56(13)5351-81(2013 Jul)
Santangelo Freel RM,Ogden KK,Strong KL,Khatri A,Chepiga KM,Jensen HS,Traynelis SF,Liotta DC

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Grin2d cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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