Gck cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gck
Entrez Gene ID	24385
Full Name	glucokinase
Synonyms	GLK,GLUKA,RNGK2
General protein information	Preferred Names glucokinase Names glucokinase HK IV HK4 glucokinase variant 3 hexokinase type IV hexokinase-4 hexokinase-D
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14q21
Summary	catalyzes the conversion of ATP and D-glucose to ADP and D-glucose 6-phosphate; may mediate glucosensing in glucose-inhibited and glucose-excited neurons [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012565.2	NP_036697.1	hexokinase-4 isoform 2
NM_001270849.1	NP_001257778.1	hexokinase-4 isoform 1
XM_006251179.3	XP_006251241.1	glucokinase isoform X1
NM_001270850.1	NP_001257779.1	hexokinase-4 isoform 3

Pathways from BioSystems

Homologies

	GCK	XP_001093035.2
Bos taurus (cattle)	GCK	NP_001095772.1
Xenopus tropicalis (tropical clawed frog)	gck	NP_001096321.1
Mus musculus (house mouse)	Gck	NP_034422.2
Rattus norvegicus (Norway rat)	Gck	NP_036697.1
Gallus gallus (chicken)	GCK	XP_427930.4
Homo sapiens (human)	GCK	NP_000153.1
Pan troglodytes (chimpanzee)	GCK	XP_001143302.1
Danio rerio (zebrafish)	gck	NP_001038850.2

Gene Ontology
Bibliography

Related articles in PubMed

Role of Islet Glucokinase, Glucose Metabolism, and Insulin Pathway in the Enhancing Effect of Islet Neogenesis-Associated Protein on Glucose-Induced Insulin Secretion.
Maiztegui B, Rom?n CL, Barbosa-Sampaio HC, Boschero AC, Gagliardino JJ
Pancreas44(6)959-66(2015 Aug)

Glucokinase activity in the arcuate nucleus regulates glucose intake.
Hussain S, Richardson E, Ma Y, Holton C, De Backer I, Buckley N, Dhillo W, Bewick G, Zhang S, Carling D, Bloom S, Gardiner J
The Journal of clinical investigation125(1)337-49(2015 Jan)

Chronic ethanol consumption inhibits glucokinase transcriptional activity by Atf3 and triggers metabolic syndrome in vivo.
Kim JY, Hwang JY, Lee DY, Song EH, Park KJ, Kim GH, Jeong EA, Lee YJ, Go MJ, Kim DJ, Lee SS, Kim BJ, Song J, Roh GS, Gao B, Kim WH
The Journal of biological chemistry289(39)27065-79(2014 Sep)

F0 maternal BPA exposure induced glucose intolerance of F2 generation through DNA methylation change in Gck.
Li G, Chang H, Xia W, Mao Z, Li Y, Xu S
Toxicology letters228(3)192-9(2014 Aug)

Chronic glucokinase activator treatment at clinically translatable exposures gives durable glucose lowering in two animal models of type 2 diabetes.
Baker DJ, Wilkinson GP, Atkinson AM, Jones HB, Coghlan M, Charles AD, Leighton B
British journal of pharmacology171(7)1642-54(2014 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The enhancing effect of INGAP-PP on glucose-induced insulin release could be partly ascribed to its effect on glucokinase activity and glucose metabolism and is mainly mediated by the PI3K/AKT pathway.
Title: Role of Islet Glucokinase, Glucose Metabolism, and Insulin Pathway in the Enhancing Effect of Islet Neogenesis-Associated Protein on Glucose-Induced Insulin Secretion.

Arcuate nucleus glucokinase activation may represent a CNS mechanism that underlies the oft-described phenomena of the "sweet tooth" and carbohydrate craving.
Title: Glucokinase activity in the arcuate nucleus regulates glucose intake.

Atf3-silencing reversed ethanol-mediated Gck down-regulation and beta-cell dysfunction, followed by the amelioration of impaired glucose tolerance and insulin resistance.
Title: Chronic ethanol consumption inhibits glucokinase transcriptional activity by Atf3 and triggers metabolic syndrome in vivo.

F0 maternal BPA exposure during gestation and lactation can induce impaired glucose homeostasis in the F2 offspring through DNA methylation change in Gck.
Title: F0 maternal BPA exposure induced glucose intolerance of F2 generation through DNA methylation change in Gck.

Chronic treatment with glucose kinase activators in animal models of diabetes provided sustained lowering of blood glucose.
Title: Chronic glucokinase activator treatment at clinically translatable exposures gives durable glucose lowering in two animal models of type 2 diabetes.

The following Gck gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gck cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12721	NM_001270849.1 Latest version!	Rattus norvegicus glucokinase (Gck), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa12722	NM_012565.2 Latest version!	Rattus norvegicus glucokinase (Gck), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa12723	NM_001270850.1 Latest version!	Rattus norvegicus glucokinase (Gck), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa36218	XM_006251179.3 Latest version!	Rattus norvegicus glucokinase (Gck), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gck ( NM_001270849.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001257778.1 Rattus norvegicus glucokinase (Gck), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa12721
Clone ID Related Accession (Same CDS sequence)	NM_001270849.1
Accession Version	NM_001270849.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	hexokinase-4 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from X53589.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X53589.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTATGG ATACTACAAG GTGTGGAGCC CAGTTGTTGA CTCTGGGCAC CAACAAATGC 
ACGAATGCAT GCTCCCTGCT GTGCAGAGCG GGTACTCACA ACGGGCACAT GAACCCTCGC 
TGCCGAACAG AGCAGGCGGC CGCCACACAG CTGCCCACCT GCAGAGTCCA GCTACTCTTG 
AACTATCATG TGGAGGGTCG AGCAGATCCT GGCAGAGTTC CAGCTGCAGG AGGAAGACCT 
GAAGAAGGTG ATGAGCCGGA TGCAGAAGGA GATGGACCGT GGCCTGAGGC TGGAGACCCA 
CGAGGAGAAG TCGGAGACTT TCTCTCCTTA GACCTGGGAG GAACCAACTT CAGAGTGATG 
CTGGTCAAAG TGGGAGAGGG GGAGGCAGGG CAGTGGAGCG TGAAGACAAA ACACCAGATG 
TACTCCATCC CCGAGGACGC CATGACGGGC ACTGCCGAGA TGCTCTTTGA CTACATCTCT 
GAATGCATCT CTGACTTCCT TGACAAGCAT CAGATGAAGC ACAAGAAACT GCCCCTGGGC 
TTCACCTTCT CCTTCCCTGT GAGGCACGAA GACCTAGACA AGGGCATCCT CCTCAATTGG 
ACCAAGGGCT TCAAGGCCTC TGGAGCAGAA GGGAACAACA TCGTAGGACT TCTCCGAGAT 
GCTATCAAGA GGAGAGGGGA CTTTGAGATG GATGTGGTGG CAATGGTGAA CGACACAGTG 
GCCACAATGA TCTCCTGCTA CTATGAAGAC CGCCAATGTG AGGTCGGCAT GATTGTGGGC 
ACTGGCTGCA ATGCCTGCTA CATGGAGGAA ATGCAGAATG TGGAGCTGGT GGAAGGGGAT 
GAGGGACGCA TGTGCGTCAA CACGGAGTGG GGCGCCTTCG GGGACTCGGG CGAGCTGGAT 
GAGTTCCTAC TGGAGTATGA CCGGATGGTG GATGAAAGCT CAGCGAACCC CGGTCAGCAG 
CTGTACGAGA AGATCATCGG TGGGAAGTAT ATGGGCGAGC TGGTACGACT TGTGCTGCTT 
AAGCTGGTGG ACGAGAACCT TCTGTTCCAC GGAGAGGCCT CGGAGCAGCT GCGCACGCGT 
GGTGCTTTTG AGACCCGTTT CGTGTCACAA GTGGAGAGCG ACTCCGGGGA CCGAAAGCAG 
ATCCACAACA TCCTAAGCAC TCTGGGGCTT CGACCCTCTG TCACCGACTG CGACATTGTG 
CGCCGTGCCT GTGAAAGCGT GTCCACTCGC GCCGCCCATA TGTGCTCCGC AGGACTAGCT 
GGGGTCATAA ATCGCATGCG CGAAAGCCGC AGTGAGGACG TGATGCGCAT CACTGTGGGC 
GTGGATGGCT CCGTGTACAA GCTGCACCCG AGCTTCAAGG AGCGGTTTCA CGCCAGTGTG 
CGCAGGCTGA CACCCAACTG CGAAATCACC TTCATCGAAT CAGAGGAGGG CAGCGGCAGG 
GGAGCCGCGC TGGTCTCTGC GGTGGCCTGC AAGAAGGCTT GCATGCTGGC CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257778.1
CDS	109..1605
Translation	MAMDTTRCGAQLLTLGTNKCTNACSLLCRAGTHNGHMNPRCRTEQAAATQLPTCRVQLLLNYHVEGRADPGRVPAAGGRPEEGDEPDAEGDGPWPEAGDPRGEVGDFLSLDLGGTNFRVMLVKVGEGEAGQWSVKTKHQMYSIPEDAMTGTAEMLFDYISECISDFLDKHQMKHKKLPLGFTFSFPVRHEDLDKGILLNWTKGFKASGAEGNNIVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDRQCEVGMIVGTGCNACYMEEMQNVELVEGDEGRMCVNTEWGAFGDSGELDEFLLEYDRMVDESSANPGQQLYEKIIGGKYMGELVRLVLLKLVDENLLFHGEASEQLRTRGAFETRFVSQVESDSGDRKQIHNILSTLGLRPSVTDCDIVRRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPNCEITFIESEEGSGRGAALVSAVACKKACMLAQ

Target ORF information:

RefSeq Version	NM_001270849.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glucokinase (Gck), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270849.1

ORF Insert Sequence:

ATGGCTATGG ATACTACAAG GTGTGGAGCC CAGTTGTTGA CTCTGGGCAC CAACAAATGC 
ACGAATGCAT GCTCCCTGCT GTGCAGAGCG GGTACTCACA ACGGGCACAT GAACCCTCGC 
TGCCGAACAG AGCAGGCGGC CGCCACACAG CTGCCCACCT GCAGAGTCCA GCTACTCTTG 
AACTATCATG TGGAGGGTCG AGCAGATCCT GGCAGAGTTC CAGCTGCAGG AGGAAGACCT 
GAAGAAGGTG ATGAGCCGGA TGCAGAAGGA GATGGACCGT GGCCTGAGGC TGGAGACCCA 
CGAGGAGAAG TCGGAGACTT TCTCTCCTTA GACCTGGGAG GAACCAACTT CAGAGTGATG 
CTGGTCAAAG TGGGAGAGGG GGAGGCAGGG CAGTGGAGCG TGAAGACAAA ACACCAGATG 
TACTCCATCC CCGAGGACGC CATGACGGGC ACTGCCGAGA TGCTCTTTGA CTACATCTCT 
GAATGCATCT CTGACTTCCT TGACAAGCAT CAGATGAAGC ACAAGAAACT GCCCCTGGGC 
TTCACCTTCT CCTTCCCTGT GAGGCACGAA GACCTAGACA AGGGCATCCT CCTCAATTGG 
ACCAAGGGCT TCAAGGCCTC TGGAGCAGAA GGGAACAACA TCGTAGGACT TCTCCGAGAT 
GCTATCAAGA GGAGAGGGGA CTTTGAGATG GATGTGGTGG CAATGGTGAA CGACACAGTG 
GCCACAATGA TCTCCTGCTA CTATGAAGAC CGCCAATGTG AGGTCGGCAT GATTGTGGGC 
ACTGGCTGCA ATGCCTGCTA CATGGAGGAA ATGCAGAATG TGGAGCTGGT GGAAGGGGAT 
GAGGGACGCA TGTGCGTCAA CACGGAGTGG GGCGCCTTCG GGGACTCGGG CGAGCTGGAT 
GAGTTCCTAC TGGAGTATGA CCGGATGGTG GATGAAAGCT CAGCGAACCC CGGTCAGCAG 
CTGTACGAGA AGATCATCGG TGGGAAGTAT ATGGGCGAGC TGGTACGACT TGTGCTGCTT 
AAGCTGGTGG ACGAGAACCT TCTGTTCCAC GGAGAGGCCT CGGAGCAGCT GCGCACGCGT 
GGTGCTTTTG AGACCCGTTT CGTGTCACAA GTGGAGAGCG ACTCCGGGGA CCGAAAGCAG 
ATCCACAACA TCCTAAGCAC TCTGGGGCTT CGACCCTCTG TCACCGACTG CGACATTGTG 
CGCCGTGCCT GTGAAAGCGT GTCCACTCGC GCCGCCCATA TGTGCTCCGC AGGACTAGCT 
GGGGTCATAA ATCGCATGCG CGAAAGCCGC AGTGAGGACG TGATGCGCAT CACTGTGGGC 
GTGGATGGCT CCGTGTACAA GCTGCACCCG AGCTTCAAGG AGCGGTTTCA CGCCAGTGTG 
CGCAGGCTGA CACCCAACTG CGAAATCACC TTCATCGAAT CAGAGGAGGG CAGCGGCAGG 
GGAGCCGCGC TGGTCTCTGC GGTGGCCTGC AAGAAGGCTT GCATGCTGGC CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gck ( NM_012565.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036697.1 Rattus norvegicus glucokinase (Gck), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa12722
Clone ID Related Accession (Same CDS sequence)	NM_012565.2
Accession Version	NM_012565.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	hexokinase-4 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from X53589.1 and X53588.1. On Aug 3, 2012 this sequence version replaced NM_012565.1. Transcript Variant: This variant (2) lacks an alternate coding exon and uses an alternate splice junction at the 3' end of another coding exon compared to variant 1. The resulting isoform (2) has the same N-and C-termini but differs in an internal segment compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X53588.1, J04218.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689598, SAMN02642793 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTATGG ATACTACAAG GTGTGGAGCC CAGTTGTTGA CTCTGGTCGA GCAGATCCTG 
GCAGAGTTCC AGCTGCAGGA GGAAGACCTG AAGAAGGTGA TGAGCCGGAT GCAGAAGGAG 
ATGGACCGTG GCCTGAGGCT GGAGACCCAC GAGGAGGCCA GTGTAAAGAT GTTACCCACC 
TACGTGCGTT CCACCCCAGA AGGCTCAGAA GTCGGAGACT TTCTCTCCTT AGACCTGGGA 
GGAACCAACT TCAGAGTGAT GCTGGTCAAA GTGGGAGAGG GGGAGGCAGG GCAGTGGAGC 
GTGAAGACAA AACACCAGAT GTACTCCATC CCCGAGGACG CCATGACGGG CACTGCCGAG 
ATGCTCTTTG ACTACATCTC TGAATGCATC TCTGACTTCC TTGACAAGCA TCAGATGAAG 
CACAAGAAAC TGCCCCTGGG CTTCACCTTC TCCTTCCCTG TGAGGCACGA AGACCTAGAC 
AAGGGCATCC TCCTCAATTG GACCAAGGGC TTCAAGGCCT CTGGAGCAGA AGGGAACAAC 
ATCGTAGGAC TTCTCCGAGA TGCTATCAAG AGGAGAGGGG ACTTTGAGAT GGATGTGGTG 
GCAATGGTGA ACGACACAGT GGCCACAATG ATCTCCTGCT ACTATGAAGA CCGCCAATGT 
GAGGTCGGCA TGATTGTGGG CACTGGCTGC AATGCCTGCT ACATGGAGGA AATGCAGAAT 
GTGGAGCTGG TGGAAGGGGA TGAGGGACGC ATGTGCGTCA ACACGGAGTG GGGCGCCTTC 
GGGGACTCGG GCGAGCTGGA TGAGTTCCTA CTGGAGTATG ACCGGATGGT GGATGAAAGC 
TCAGCGAACC CCGGTCAGCA GCTGTACGAG AAGATCATCG GTGGGAAGTA TATGGGCGAG 
CTGGTACGAC TTGTGCTGCT TAAGCTGGTG GACGAGAACC TTCTGTTCCA CGGAGAGGCC 
TCGGAGCAGC TGCGCACGCG TGGTGCTTTT GAGACCCGTT TCGTGTCACA AGTGGAGAGC 
GACTCCGGGG ACCGAAAGCA GATCCACAAC ATCCTAAGCA CTCTGGGGCT TCGACCCTCT 
GTCACCGACT GCGACATTGT GCGCCGTGCC TGTGAAAGCG TGTCCACTCG CGCCGCCCAT 
ATGTGCTCCG CAGGACTAGC TGGGGTCATA AATCGCATGC GCGAAAGCCG CAGTGAGGAC 
GTGATGCGCA TCACTGTGGG CGTGGATGGC TCCGTGTACA AGCTGCACCC GAGCTTCAAG 
GAGCGGTTTC ACGCCAGTGT GCGCAGGCTG ACACCCAACT GCGAAATCAC CTTCATCGAA 
TCAGAGGAGG GCAGCGGCAG GGGAGCCGCG CTGGTCTCTG CGGTGGCCTG CAAGAAGGCT 
TGCATGCTGG CCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036697.1
CDS	109..1506
Translation	MAMDTTRCGAQLLTLVEQILAEFQLQEEDLKKVMSRMQKEMDRGLRLETHEEASVKMLPTYVRSTPEGSEVGDFLSLDLGGTNFRVMLVKVGEGEAGQWSVKTKHQMYSIPEDAMTGTAEMLFDYISECISDFLDKHQMKHKKLPLGFTFSFPVRHEDLDKGILLNWTKGFKASGAEGNNIVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDRQCEVGMIVGTGCNACYMEEMQNVELVEGDEGRMCVNTEWGAFGDSGELDEFLLEYDRMVDESSANPGQQLYEKIIGGKYMGELVRLVLLKLVDENLLFHGEASEQLRTRGAFETRFVSQVESDSGDRKQIHNILSTLGLRPSVTDCDIVRRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPNCEITFIESEEGSGRGAALVSAVACKKACMLAQ

Target ORF information:

RefSeq Version	NM_012565.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glucokinase (Gck), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012565.2

ORF Insert Sequence:

ATGGCTATGG ATACTACAAG GTGTGGAGCC CAGTTGTTGA CTCTGGTCGA GCAGATCCTG 
GCAGAGTTCC AGCTGCAGGA GGAAGACCTG AAGAAGGTGA TGAGCCGGAT GCAGAAGGAG 
ATGGACCGTG GCCTGAGGCT GGAGACCCAC GAGGAGGCCA GTGTAAAGAT GTTACCCACC 
TACGTGCGTT CCACCCCAGA AGGCTCAGAA GTCGGAGACT TTCTCTCCTT AGACCTGGGA 
GGAACCAACT TCAGAGTGAT GCTGGTCAAA GTGGGAGAGG GGGAGGCAGG GCAGTGGAGC 
GTGAAGACAA AACACCAGAT GTACTCCATC CCCGAGGACG CCATGACGGG CACTGCCGAG 
ATGCTCTTTG ACTACATCTC TGAATGCATC TCTGACTTCC TTGACAAGCA TCAGATGAAG 
CACAAGAAAC TGCCCCTGGG CTTCACCTTC TCCTTCCCTG TGAGGCACGA AGACCTAGAC 
AAGGGCATCC TCCTCAATTG GACCAAGGGC TTCAAGGCCT CTGGAGCAGA AGGGAACAAC 
ATCGTAGGAC TTCTCCGAGA TGCTATCAAG AGGAGAGGGG ACTTTGAGAT GGATGTGGTG 
GCAATGGTGA ACGACACAGT GGCCACAATG ATCTCCTGCT ACTATGAAGA CCGCCAATGT 
GAGGTCGGCA TGATTGTGGG CACTGGCTGC AATGCCTGCT ACATGGAGGA AATGCAGAAT 
GTGGAGCTGG TGGAAGGGGA TGAGGGACGC ATGTGCGTCA ACACGGAGTG GGGCGCCTTC 
GGGGACTCGG GCGAGCTGGA TGAGTTCCTA CTGGAGTATG ACCGGATGGT GGATGAAAGC 
TCAGCGAACC CCGGTCAGCA GCTGTACGAG AAGATCATCG GTGGGAAGTA TATGGGCGAG 
CTGGTACGAC TTGTGCTGCT TAAGCTGGTG GACGAGAACC TTCTGTTCCA CGGAGAGGCC 
TCGGAGCAGC TGCGCACGCG TGGTGCTTTT GAGACCCGTT TCGTGTCACA AGTGGAGAGC 
GACTCCGGGG ACCGAAAGCA GATCCACAAC ATCCTAAGCA CTCTGGGGCT TCGACCCTCT 
GTCACCGACT GCGACATTGT GCGCCGTGCC TGTGAAAGCG TGTCCACTCG CGCCGCCCAT 
ATGTGCTCCG CAGGACTAGC TGGGGTCATA AATCGCATGC GCGAAAGCCG CAGTGAGGAC 
GTGATGCGCA TCACTGTGGG CGTGGATGGC TCCGTGTACA AGCTGCACCC GAGCTTCAAG 
GAGCGGTTTC ACGCCAGTGT GCGCAGGCTG ACACCCAACT GCGAAATCAC CTTCATCGAA 
TCAGAGGAGG GCAGCGGCAG GGGAGCCGCG CTGGTCTCTG CGGTGGCCTG CAAGAAGGCT 
TGCATGCTGG CCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gck ( NM_001270850.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001257779.1 Rattus norvegicus glucokinase (Gck), transcript variant 3, mRNA.

Price & Availability↑

CloneID	ORa12723
Clone ID Related Accession (Same CDS sequence)	NM_001270850.1
Accession Version	NM_001270850.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1347bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	hexokinase-4 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC110110.5 and X53588.1. Transcript Variant: This variant (3) differs in the 5' UTR and coding region and uses two alternate splice junctions compared to variant 1. The resulting isoform (3) has a shorter and distinct N-terminus and contains two alternate internal segments compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M25807.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGGATG ACAGAGCCAG GATGGAGGCC ACCAAGAAGG AAAAGGTCGA GCAGATCCTG 
GCAGAGTTCC AGCTGCAGGA GGAAGACCTG AAGAAGGTGA TGAGCCGGAT GCAGAAGGAG 
ATGGACCGTG GCCTGAGGCT GGAGACCCAC GAGGAGGCCA GTGTAAAGAT GTTACCCACC 
TACGTGCGTT CCACCCCAGA AGGCTCAGAA GTCGGAGACT TTCTCTCCTT AGACCTGGGA 
GGAACCAACT TCAGAGTGAT GCTGGTCAAA GTGGGAGAGG GGGAGGCAGG GCAGTGGAGC 
GTGAAGACAA AACACCAGAT GTACTCCATC CCCGAGGACG CCATGACGGG CACTGCCGAG 
ATGATGAAGC ACAAGAAACT GCCCCTGGGC TTCACCTTCT CCTTCCCTGT GAGGCACGAA 
GACCTAGACA AGGGCATCCT CCTCAATTGG ACCAAGGGCT TCAAGGCCTC TGGAGCAGAA 
GGGAACAACA TCGTAGGACT TCTCCGAGAT GCTATCAAGA GGAGAGGGGA CTTTGAGATG 
GATGTGGTGG CAATGGTGAA CGACACAGTG GCCACAATGA TCTCCTGCTA CTATGAAGAC 
CGCCAATGTG AGGTCGGCAT GATTGTGGGC ACTGGCTGCA ATGCCTGCTA CATGGAGGAA 
ATGCAGAATG TGGAGCTGGT GGAAGGGGAT GAGGGACGCA TGTGCGTCAA CACGGAGTGG 
GGCGCCTTCG GGGACTCGGG CGAGCTGGAT GAGTTCCTAC TGGAGTATGA CCGGATGGTG 
GATGAAAGCT CAGCGAACCC CGGTCAGCAG CTGTACGAGA AGATCATCGG TGGGAAGTAT 
ATGGGCGAGC TGGTACGACT TGTGCTGCTT AAGCTGGTGG ACGAGAACCT TCTGTTCCAC 
GGAGAGGCCT CGGAGCAGCT GCGCACGCGT GGTGCTTTTG AGACCCGTTT CGTGTCACAA 
GTGGAGAGCG ACTCCGGGGA CCGAAAGCAG ATCCACAACA TCCTAAGCAC TCTGGGGCTT 
CGACCCTCTG TCACCGACTG CGACATTGTG CGCCGTGCCT GTGAAAGCGT GTCCACTCGC 
GCCGCCCATA TGTGCTCCGC AGGACTAGCT GGGGTCATAA ATCGCATGCG CGAAAGCCGC 
AGTGAGGACG TGATGCGCAT CACTGTGGGC GTGGATGGCT CCGTGTACAA GCTGCACCCG 
AGCTTCAAGG AGCGGTTTCA CGCCAGTGTG CGCAGGCTGA CACCCAACTG CGAAATCACC 
TTCATCGAAT CAGAGGAGGG CAGCGGCAGG GGAGCCGCGC TGGTCTCTGC GGTGGCCTGC 
AAGAAGGCTT GCATGCTGGC CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257779.1
CDS	37..1383
Translation	MLDDRARMEATKKEKVEQILAEFQLQEEDLKKVMSRMQKEMDRGLRLETHEEASVKMLPTYVRSTPEGSEVGDFLSLDLGGTNFRVMLVKVGEGEAGQWSVKTKHQMYSIPEDAMTGTAEMMKHKKLPLGFTFSFPVRHEDLDKGILLNWTKGFKASGAEGNNIVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDRQCEVGMIVGTGCNACYMEEMQNVELVEGDEGRMCVNTEWGAFGDSGELDEFLLEYDRMVDESSANPGQQLYEKIIGGKYMGELVRLVLLKLVDENLLFHGEASEQLRTRGAFETRFVSQVESDSGDRKQIHNILSTLGLRPSVTDCDIVRRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPNCEITFIESEEGSGRGAALVSAVACKKACMLAQ

Target ORF information:

RefSeq Version	NM_001270850.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glucokinase (Gck), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270850.1

ORF Insert Sequence:

ATGCTGGATG ACAGAGCCAG GATGGAGGCC ACCAAGAAGG AAAAGGTCGA GCAGATCCTG 
GCAGAGTTCC AGCTGCAGGA GGAAGACCTG AAGAAGGTGA TGAGCCGGAT GCAGAAGGAG 
ATGGACCGTG GCCTGAGGCT GGAGACCCAC GAGGAGGCCA GTGTAAAGAT GTTACCCACC 
TACGTGCGTT CCACCCCAGA AGGCTCAGAA GTCGGAGACT TTCTCTCCTT AGACCTGGGA 
GGAACCAACT TCAGAGTGAT GCTGGTCAAA GTGGGAGAGG GGGAGGCAGG GCAGTGGAGC 
GTGAAGACAA AACACCAGAT GTACTCCATC CCCGAGGACG CCATGACGGG CACTGCCGAG 
ATGATGAAGC ACAAGAAACT GCCCCTGGGC TTCACCTTCT CCTTCCCTGT GAGGCACGAA 
GACCTAGACA AGGGCATCCT CCTCAATTGG ACCAAGGGCT TCAAGGCCTC TGGAGCAGAA 
GGGAACAACA TCGTAGGACT TCTCCGAGAT GCTATCAAGA GGAGAGGGGA CTTTGAGATG 
GATGTGGTGG CAATGGTGAA CGACACAGTG GCCACAATGA TCTCCTGCTA CTATGAAGAC 
CGCCAATGTG AGGTCGGCAT GATTGTGGGC ACTGGCTGCA ATGCCTGCTA CATGGAGGAA 
ATGCAGAATG TGGAGCTGGT GGAAGGGGAT GAGGGACGCA TGTGCGTCAA CACGGAGTGG 
GGCGCCTTCG GGGACTCGGG CGAGCTGGAT GAGTTCCTAC TGGAGTATGA CCGGATGGTG 
GATGAAAGCT CAGCGAACCC CGGTCAGCAG CTGTACGAGA AGATCATCGG TGGGAAGTAT 
ATGGGCGAGC TGGTACGACT TGTGCTGCTT AAGCTGGTGG ACGAGAACCT TCTGTTCCAC 
GGAGAGGCCT CGGAGCAGCT GCGCACGCGT GGTGCTTTTG AGACCCGTTT CGTGTCACAA 
GTGGAGAGCG ACTCCGGGGA CCGAAAGCAG ATCCACAACA TCCTAAGCAC TCTGGGGCTT 
CGACCCTCTG TCACCGACTG CGACATTGTG CGCCGTGCCT GTGAAAGCGT GTCCACTCGC 
GCCGCCCATA TGTGCTCCGC AGGACTAGCT GGGGTCATAA ATCGCATGCG CGAAAGCCGC 
AGTGAGGACG TGATGCGCAT CACTGTGGGC GTGGATGGCT CCGTGTACAA GCTGCACCCG 
AGCTTCAAGG AGCGGTTTCA CGCCAGTGTG CGCAGGCTGA CACCCAACTG CGAAATCACC 
TTCATCGAAT CAGAGGAGGG CAGCGGCAGG GGAGCCGCGC TGGTCTCTGC GGTGGCCTGC 
AAGAAGGCTT GCATGCTGGC CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gck ( XM_006251179.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006251241.1 Rattus norvegicus glucokinase (Gck), transcript variant X1, mRNA.

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CloneID	ORa36218
Clone ID Related Accession (Same CDS sequence)	XM_006251179.3
Accession Version	XM_006251179.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	glucokinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006251179.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGATG ACAGAGCCAG GATGGAGGCC ACCAAGAAGG AAAAGGTCGA GCAGATCCTG 
GCAGAGTTCC AGCTGCAGGA GGAAGACCTG AAGAAGGTGA TGAGCCGGAT GCAGAAGGAG 
ATGGACCGTG GCCTGAGGCT GGAGACCCAC GAGGAGGCCA GTGTAAAGAT GTTACCCACC 
TACGTGCGTT CCACCCCAGA AGGCTCAGAA GTCGGAGACT TTCTCTCCTT AGACCTGGGA 
GGAACCAACT TCAGAGTGAT GCTGGTCAAA GTGGGAGAGG GGGAGGCAGG GCAGTGGAGC 
GTGAAGACAA AACACCAGAT GTACTCCATC CCCGAGGACG CCATGACGGG CACTGCCGAG 
ATGCTCTTTG ACTACATCTC TGAATGCATC TCTGACTTCC TTGACAAGCA TCAGATGAAG 
CACAAGAAAC TGCCCCTGGG CTTCACCTTC TCCTTCCCTG TGAGGCACGA AGACCTAGAC 
AAGGGCATCC TCCTCAATTG GACCAAGGGC TTCAAGGCCT CTGGAGCAGA AGGGAACAAC 
ATCGTAGGAC TTCTCCGAGA TGCTATCAAG AGGAGAGGGG ACTTTGAGAT GGATGTGGTG 
GCAATGGTGA ACGACACAGT GGCCACAATG ATCTCCTGCT ACTATGAAGA CCGCCAATGT 
GAGGTCGGCA TGATTGTGGG CACTGGCTGC AATGCCTGCT ACATGGAGGA AATGCAGAAT 
GTGGAGCTGG TGGAAGGGGA TGAGGGACGC ATGTGCGTCA ACACGGAGTG GGGCGCCTTC 
GGGGACTCGG GCGAGCTGGA TGAGTTCCTA CTGGAGTATG ACCGGATGGT GGATGAAAGC 
TCAGCGAACC CCGGTCAGCA GCTGTACGAG AAGATCATCG GTGGGAAGTA TATGGGCGAG 
CTGGTACGAC TTGTGCTGCT TAAGCTGGTG GACGAGAACC TTCTGTTCCA CGGAGAGGCC 
TCGGAGCAGC TGCGCACGCG TGGTGCTTTT GAGACCCGTT TCGTGTCACA AGTGGAGAGC 
GACTCCGGGG ACCGAAAGCA GATCCACAAC ATCCTAAGCA CTCTGGGGCT TCGACCCTCT 
GTCACCGACT GCGACATTGT GCGCCGTGCC TGTGAAAGCG TGTCCACTCG CGCCGCCCAT 
ATGTGCTCCG CAGGACTAGC TGGGGTCATA AATCGCATGC GCGAAAGCCG CAGTGAGGAC 
GTGATGCGCA TCACTGTGGG CGTGGATGGC TCCGTGTACA AGCTGCACCC GAGCTTCAAG 
GAGCGGTTTC ACGCCAGTGT GCGCAGGCTG ACACCCAACT GCGAAATCAC CTTCATCGAA 
TCAGAGGAGG GCAGCGGCAG GGGAGCCGCG CTGGTCTCTG CGGTGGCCTG CAAGAAGGCT 
TGCATGCTGG CCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006251241.1
CDS	967..2364
Translation	MLDDRARMEATKKEKVEQILAEFQLQEEDLKKVMSRMQKEMDRGLRLETHEEASVKMLPTYVRSTPEGSEVGDFLSLDLGGTNFRVMLVKVGEGEAGQWSVKTKHQMYSIPEDAMTGTAEMLFDYISECISDFLDKHQMKHKKLPLGFTFSFPVRHEDLDKGILLNWTKGFKASGAEGNNIVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDRQCEVGMIVGTGCNACYMEEMQNVELVEGDEGRMCVNTEWGAFGDSGELDEFLLEYDRMVDESSANPGQQLYEKIIGGKYMGELVRLVLLKLVDENLLFHGEASEQLRTRGAFETRFVSQVESDSGDRKQIHNILSTLGLRPSVTDCDIVRRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPNCEITFIESEEGSGRGAALVSAVACKKACMLAQ

Target ORF information:

RefSeq Version	XM_006251179.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glucokinase (Gck), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006251179.3

ORF Insert Sequence:

ATGCTGGATG ACAGAGCCAG GATGGAGGCC ACCAAGAAGG AAAAGGTCGA GCAGATCCTG 
GCAGAGTTCC AGCTGCAGGA GGAAGACCTG AAGAAGGTGA TGAGCCGGAT GCAGAAGGAG 
ATGGACCGTG GCCTGAGGCT GGAGACCCAC GAGGAGGCCA GTGTAAAGAT GTTACCCACC 
TACGTGCGTT CCACCCCAGA AGGCTCAGAA GTCGGAGACT TTCTCTCCTT AGACCTGGGA 
GGAACCAACT TCAGAGTGAT GCTGGTCAAA GTGGGAGAGG GGGAGGCAGG GCAGTGGAGC 
GTGAAGACAA AACACCAGAT GTACTCCATC CCCGAGGACG CCATGACGGG CACTGCCGAG 
ATGCTCTTTG ACTACATCTC TGAATGCATC TCTGACTTCC TTGACAAGCA TCAGATGAAG 
CACAAGAAAC TGCCCCTGGG CTTCACCTTC TCCTTCCCTG TGAGGCACGA AGACCTAGAC 
AAGGGCATCC TCCTCAATTG GACCAAGGGC TTCAAGGCCT CTGGAGCAGA AGGGAACAAC 
ATCGTAGGAC TTCTCCGAGA TGCTATCAAG AGGAGAGGGG ACTTTGAGAT GGATGTGGTG 
GCAATGGTGA ACGACACAGT GGCCACAATG ATCTCCTGCT ACTATGAAGA CCGCCAATGT 
GAGGTCGGCA TGATTGTGGG CACTGGCTGC AATGCCTGCT ACATGGAGGA AATGCAGAAT 
GTGGAGCTGG TGGAAGGGGA TGAGGGACGC ATGTGCGTCA ACACGGAGTG GGGCGCCTTC 
GGGGACTCGG GCGAGCTGGA TGAGTTCCTA CTGGAGTATG ACCGGATGGT GGATGAAAGC 
TCAGCGAACC CCGGTCAGCA GCTGTACGAG AAGATCATCG GTGGGAAGTA TATGGGCGAG 
CTGGTACGAC TTGTGCTGCT TAAGCTGGTG GACGAGAACC TTCTGTTCCA CGGAGAGGCC 
TCGGAGCAGC TGCGCACGCG TGGTGCTTTT GAGACCCGTT TCGTGTCACA AGTGGAGAGC 
GACTCCGGGG ACCGAAAGCA GATCCACAAC ATCCTAAGCA CTCTGGGGCT TCGACCCTCT 
GTCACCGACT GCGACATTGT GCGCCGTGCC TGTGAAAGCG TGTCCACTCG CGCCGCCCAT 
ATGTGCTCCG CAGGACTAGC TGGGGTCATA AATCGCATGC GCGAAAGCCG CAGTGAGGAC 
GTGATGCGCA TCACTGTGGG CGTGGATGGC TCCGTGTACA AGCTGCACCC GAGCTTCAAG 
GAGCGGTTTC ACGCCAGTGT GCGCAGGCTG ACACCCAACT GCGAAATCAC CTTCATCGAA 
TCAGAGGAGG GCAGCGGCAG GGGAGCCGCG CTGGTCTCTG CGGTGGCCTG CAAGAAGGCT 
TGCATGCTGG CCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of Islet Glucokinase, Glucose Metabolism, and Insulin Pathway in the Enhancing Effect of Islet Neogenesis-Associated Protein on Glucose-Induced Insulin Secretion.
Pancreas44(6)959-66(2015 Aug)
Maiztegui B,Rom?n CL,Barbosa-Sampaio HC,Boschero AC,Gagliardino JJ

Glucokinase activity in the arcuate nucleus regulates glucose intake.
The Journal of clinical investigation125(1)337-49(2015 Jan)
Hussain S,Richardson E,Ma Y,Holton C,De Backer I,Buckley N,Dhillo W,Bewick G,Zhang S,Carling D,Bloom S,Gardiner J

Chronic ethanol consumption inhibits glucokinase transcriptional activity by Atf3 and triggers metabolic syndrome in vivo.
The Journal of biological chemistry289(39)27065-79(2014 Sep)
Kim JY,Hwang JY,Lee DY,Song EH,Park KJ,Kim GH,Jeong EA,Lee YJ,Go MJ,Kim DJ,Lee SS,Kim BJ,Song J,Roh GS,Gao B,Kim WH

F0 maternal BPA exposure induced glucose intolerance of F2 generation through DNA methylation change in Gck.
Toxicology letters228(3)192-9(2014 Aug)
Li G,Chang H,Xia W,Mao Z,Li Y,Xu S

Chronic glucokinase activator treatment at clinically translatable exposures gives durable glucose lowering in two animal models of type 2 diabetes.
British journal of pharmacology171(7)1642-54(2014 Apr)
Baker DJ,Wilkinson GP,Atkinson AM,Jones HB,Coghlan M,Charles AD,Leighton B

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Gck cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

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電話

FAX

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