The following Gck gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gck cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa12721 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001270849.1 | |
| Accession Version | NM_001270849.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1497bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-09-21 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | hexokinase-4 isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from X53589.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X53589.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGCTATGG ATACTACAAG GTGTGGAGCC CAGTTGTTGA CTCTGGGCAC CAACAAATGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001257778.1 |
| CDS | 109..1605 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001270849.1 |
1 | ATGGCTATGG ATACTACAAG GTGTGGAGCC CAGTTGTTGA CTCTGGGCAC CAACAAATGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa12722 | |
| Clone ID Related Accession (Same CDS sequence) | NM_012565.2 | |
| Accession Version | NM_012565.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1398bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-09-21 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | hexokinase-4 isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from X53589.1 and X53588.1. On Aug 3, 2012 this sequence version replaced NM_012565.1. Transcript Variant: This variant (2) lacks an alternate coding exon and uses an alternate splice junction at the 3' end of another coding exon compared to variant 1. The resulting isoform (2) has the same N-and C-termini but differs in an internal segment compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X53588.1, J04218.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689598, SAMN02642793 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGCTATGG ATACTACAAG GTGTGGAGCC CAGTTGTTGA CTCTGGTCGA GCAGATCCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_036697.1 |
| CDS | 109..1506 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_012565.2 |
1 | ATGGCTATGG ATACTACAAG GTGTGGAGCC CAGTTGTTGA CTCTGGTCGA GCAGATCCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa12723 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001270850.1 | |
| Accession Version | NM_001270850.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1347bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-09-21 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | hexokinase-4 isoform 3 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC110110.5 and X53588.1. Transcript Variant: This variant (3) differs in the 5' UTR and coding region and uses two alternate splice junctions compared to variant 1. The resulting isoform (3) has a shorter and distinct N-terminus and contains two alternate internal segments compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M25807.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGCTGGATG ACAGAGCCAG GATGGAGGCC ACCAAGAAGG AAAAGGTCGA GCAGATCCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001257779.1 |
| CDS | 37..1383 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001270850.1 |
1 | ATGCTGGATG ACAGAGCCAG GATGGAGGCC ACCAAGAAGG AAAAGGTCGA GCAGATCCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa36218 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006251179.3 | |
| Accession Version | XM_006251179.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1398bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | glucokinase isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006251179.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCTGGATG ACAGAGCCAG GATGGAGGCC ACCAAGAAGG AAAAGGTCGA GCAGATCCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006251241.1 |
| CDS | 967..2364 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006251179.3 |
1 | ATGCTGGATG ACAGAGCCAG GATGGAGGCC ACCAAGAAGG AAAAGGTCGA GCAGATCCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Role of Islet Glucokinase, Glucose Metabolism, and Insulin Pathway in the Enhancing Effect of Islet Neogenesis-Associated Protein on Glucose-Induced Insulin Secretion. |
Glucokinase activity in the arcuate nucleus regulates glucose intake. |
Chronic ethanol consumption inhibits glucokinase transcriptional activity by Atf3 and triggers metabolic syndrome in vivo. |
F0 maternal BPA exposure induced glucose intolerance of F2 generation through DNA methylation change in Gck. |
Chronic glucokinase activator treatment at clinically translatable exposures gives durable glucose lowering in two animal models of type 2 diabetes. |
