G6pd cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	G6pd
Entrez Gene ID	24377
Full Name	glucose-6-phosphate dehydrogenase
Synonyms	G6pdx
General protein information	Preferred Names glucose-6-phosphate dehydrogenase Names glucose-6-phosphate 1-dehydrogenase glucose-6-phosphate dehydrogenase X-linked
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq37
Summary	catalyzes the first rate-limiting step in the pentose phosphate pathway; may mediate peroxynitrite functions in neuroprotection against nitric oxide-mediated apoptosis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017006.2	NP_058702.1	glucose-6-phosphate 1-dehydrogenase

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010739	XP_559252.3
Caenorhabditis elegans (roundworm)	gspd-1	NP_502129.1
Oryza sativa (rice)	Os02g0600400	NP_001047345.1
Oryza sativa (rice)	Os04g0485300	NP_001053132.1
Homo sapiens (human)	G6PD	NP_000393.4
	G6PD	XP_001095382.1
Canis lupus familiaris (dog)	G6PD	XP_538209.2
Drosophila melanogaster (fruit fly)	Zw	NP_523411.1
Schizosaccharomyces pombe (fission yeast)	zwf1	NP_593344.2
Rattus norvegicus (Norway rat)	G6pd	NP_058702.1
Saccharomyces cerevisiae (baker's yeast)	ZWF1	NP_014158.1
Eremothecium gossypii	AGOS_ABL206C	NP_982741.1
Magnaporthe oryzae (rice blast fungus)	MGG_09926	XP_003710026.1
Arabidopsis thaliana (thale cress)	G6PD6	NP_198892.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012678	XP_307095.4
Kluyveromyces lactis	KLLA0D19855g	XP_453944.1
Arabidopsis thaliana (thale cress)	G6PD5	NP_001030780.1
Xenopus tropicalis (tropical clawed frog)	g6pd	NP_001017312.1
Bos taurus (cattle)	G6PD	NP_001231064.1
Mus musculus (house mouse)	G6pdx	NP_032088.1
Danio rerio (zebrafish)	g6pd	XP_699168.3
Neurospora crassa	NCU09111	XP_958320.2

Gene Ontology
Bibliography

Related articles in PubMed

Purification of glucose-6-phosphate dehydrogenase from rat (Rattus norvegicus) erythrocytes and inhibition effects of some metal ions on enzyme activity.
Temel Y, Kocyigit UM
Journal of biochemical and molecular toxicology31(9)(2017 Sep)

20-HETE-induced mitochondrial superoxide production and inflammatory phenotype in vascular smooth muscle is prevented by glucose-6-phosphate dehydrogenase inhibition.
Lakhkar A, Dhagia V, Joshi SR, Gotlinger K, Patel D, Sun D, Wolin MS, Schwartzman ML, Gupte SA
American journal of physiology. Heart and circulatory physiology310(9)H1107-17(2016 May)

G6PD deficiency and absence of ?-thalassemia increase the risk for cerebral vasculopathy in children with sickle cell anemia.
Joly P, Garnier N, Kebaili K, Renoux C, Dony A, Cheikh N, Renard C, Ceraulo A, Cuzzubbo D, Pondarr? C, Martin C, Pialoux V, Francina A, Bertrand Y, Connes P
European journal of haematology96(4)404-8(2016 Apr)

Hypoxia-induced glucose-6-phosphate dehydrogenase overexpression and -activation in pulmonary artery smooth muscle cells: implication in pulmonary hypertension.
Chettimada S, Gupte R, Rawat D, Gebb SA, McMurtry IF, Gupte SA
American journal of physiology. Lung cellular and molecular physiology308(3)L287-300(2015 Feb)

DNA sequence abnormalities of human glucose-6-phosphate dehydrogenase variants.
Beutler E, Kuhl W, Gelbart T, Forman L
The Journal of biological chemistry266(7)4145-50(1991 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

an investigation was carried out regarding the inhibitory effects caused by various metal ions (Fe(2+) , Pb(2+) , Cd(2+) , Ag(+) , and Zn(2+) ) on G6PD enzyme activities, as per Beutler method at 340 nm under in vitro conditions. Lineweaver-Burk diagrams were used for estimation of the IC50 and Ki values for the metals. Ki values for Pb(+2) , Cd(+2) , Ag(+) , and Zn(+2) were 113.3, 215.2, 19.4, and 474.7 muM, respectiv...
Title: Purification of glucose-6-phosphate dehydrogenase from rat (Rattus norvegicus) erythrocytes and inhibition effects of some metal ions on enzyme activity.

20-HETE elicited mitochondrial superoxide production and promoted secretory phenotype of vascular smooth muscle cells by activating MAPK1-Elk-1, all of which are blocked by inhibition of G6PD.
Title: 20-HETE-induced mitochondrial superoxide production and inflammatory phenotype in vascular smooth muscle is prevented by glucose-6-phosphate dehydrogenase inhibition.

Altered glucose metabolism and G6PD overactivation play a key role in switching the pulmonary artery smooth muscle cells from the contractile to synthetic phenotype.
Title: Hypoxia-induced glucose-6-phosphate dehydrogenase overexpression and -activation in pulmonary artery smooth muscle cells: implication in pulmonary hypertension.

Examination of changes in enzyme activities of erythrocyte glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase in rats given Naringenin and Lead acetate.
Title: Examination of changes in enzyme activities of erythrocyte glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase in rats given Naringenin and Lead acetate.

Glucose-6-phosphate dehydrogenase alleviated the signs of behavioural and biochemical effects of AlCl3-treatment suggesting its involvement in the pathogenesis of Aluminum neurotoxicity and its potential therapeutic benefit.
Title: Protective effects of glucose-6-phosphate dehydrogenase on neurotoxicity of aluminium applied into the CA1 sector of rat hippocampus.

The following G6pd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the G6pd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12070	NM_017006.2 Latest version!	Rattus norvegicus glucose-6-phosphate dehydrogenase (G6pd), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$300.30 $429.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

G6pd ( NM_017006.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058702.1 Rattus norvegicus glucose-6-phosphate dehydrogenase (G6pd), mRNA.

Price & Availability↑

CloneID	ORa12070
Clone ID Related Accession (Same CDS sequence)	NM_017006.2
Accession Version	NM_017006.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	glucose-6-phosphate 1-dehydrogenase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB729647.1 and BC081820.1. On Oct 13, 2007 this sequence version replaced NM_017006.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC081820.1, X07467.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGAGC AGGTGGCTTT GAGCCGGACC CAGGTGTGTG GGATCCTGAG GGAAGAGTTG 
TACCAGGGTG ATGCCTTCCA CCAAGCTGAT ACACACATAT TTATCATCAT GGGTGCATCG 
GGTGACCTGG CCAAGAAGAA GATTTATCCT ACCATCTGGT GGCTGTTCCG GGATGGCCTT 
CTACCCGAAG ACACCTTCAT TGTAGGCTAT GCCCGCTCAC GACTCACAGT GGATGACATC 
CGCAAACAGA GTGAGCCCTT CTTTAAAGTC ACTCCAGAAG AAAGACCCAA GCTAGAGGAG 
TTCTTTGCCC GTAACTCCTA TGTAGCTGGC CAGTATGATG ATCCAGCCTC CTACAAGCAC 
CTCAACAGCC ACATGAATGC CCTGCACCAG GGAATGCAGG CCAACCGTCT GTTCTACCTG 
GCCTTGCCCC CCACTGTCTA TGAAGCAGTC ACCAAGAACA TTCAAGAGAT CTGCATGAGT 
CAGACAGGCT GGAACCGCAT CATAGTGGAG AAGCCCTTCG GGAGAGACCT GCAGAGCTCC 
AATCAACTGT CGAACCACAT CTCCTCTCTG TTTCGTGAGG ACCAGATCTA CCGCATTGAC 
CACTACCTGG GCAAAGAGAT GGTCCAGAAC CTCATGGTGC TGAGATTTGC CAACAGGATC 
TTTGGACCCA TCTGGAATCG AGACAACATT GCTTGTGTGA TCCTTACATT TAAAGAGCCC 
TTTGGTACTG AGGGTCGTGG GGGCTATTTT GATGAATTTG GGATCATCAG GGATGTCATG 
CAGAACCACC TCCTGCAGAT GTTGTGTCTA GTGGCCATGG AAAAGCCTGC CTCTACAGAT 
TCAGATGATG TCCGTGATGA GAAGGTCAAA GTGTTAAAAT GTATCTCAGA GGTGGAAACT 
GACAACGTGG TCCTTGGCCA GTATGTGGGG AACCCCAGTG GAGAAGGAGA AGCTACCAAT 
GGGTACTTAG ATGACCCCAC AGTACCCCAT GGGTCTACCA CTGCTACCTT TGCAGCAGCT 
GTCCTCTATG TGGAGAATGA ACGGTGGGAT GGAGTACCCT TCATCCTGCG CTGTGGCAAA 
GCTCTGAATG AGCGCAAAGC TGAAGTGAGA CTTCAGTTCC GCGATGTGGC AGGTGACATC 
TTCCACCAGC AGTGCAAGCG TAACGAGCTG GTCATCCGTG TGCAGCCCAA TGAGGCGGTA 
TACACCAAGA TGATGACCAA GAAGCCTGGC ATGTTCTTCA ACCCTGAGGA GTCTGAGCTG 
GACCTAACCT ATGGCAACAG ATACAAGAAT GTGAAGCTCC CTGATGCCTA TGAACGCCTC 
ATCCTGGATG TCTTCTGTGG GAGCCAAATG CACTTTGTCC GTAGTGATGA ACTCAGGGAA 
GCCTGGCGTA TCTTCACACC ATTGCTGCAC AAGATTGATC GAGAGAAGCC CCAGCCCATC 
CCGTATGTCT ATGGCAGCCG AGGTCCCACA GAGGCAGATG AGCTGATGAA GAGAGTGGGC 
TTCCAGTATG AGGGTACCTA CAAGTGGGTG AACCCTCACA AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058702.1
CDS	116..1663
Translation	MAEQVALSRTQVCGILREELYQGDAFHQADTHIFIIMGASGDLAKKKIYPTIWWLFRDGLLPEDTFIVGYARSRLTVDDIRKQSEPFFKVTPEERPKLEEFFARNSYVAGQYDDPASYKHLNSHMNALHQGMQANRLFYLALPPTVYEAVTKNIQEICMSQTGWNRIIVEKPFGRDLQSSNQLSNHISSLFREDQIYRIDHYLGKEMVQNLMVLRFANRIFGPIWNRDNIACVILTFKEPFGTEGRGGYFDEFGIIRDVMQNHLLQMLCLVAMEKPASTDSDDVRDEKVKVLKCISEVETDNVVLGQYVGNPSGEGEATNGYLDDPTVPHGSTTATFAAAVLYVENERWDGVPFILRCGKALNERKAEVRLQFRDVAGDIFHQQCKRNELVIRVQPNEAVYTKMMTKKPGMFFNPEESELDLTYGNRYKNVKLPDAYERLILDVFCGSQMHFVRSDELREAWRIFTPLLHKIDREKPQPIPYVYGSRGPTEADELMKRVGFQYEGTYKWVNPHKL

Target ORF information:

RefSeq Version	NM_017006.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glucose-6-phosphate dehydrogenase (G6pd), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017006.2

ORF Insert Sequence:

ATGGCAGAGC AGGTGGCTTT GAGCCGGACC CAGGTGTGTG GGATCCTGAG GGAAGAGTTG 
TACCAGGGTG ATGCCTTCCA CCAAGCTGAT ACACACATAT TTATCATCAT GGGTGCATCG 
GGTGACCTGG CCAAGAAGAA GATTTATCCT ACCATCTGGT GGCTGTTCCG GGATGGCCTT 
CTACCCGAAG ACACCTTCAT TGTAGGCTAT GCCCGCTCAC GACTCACAGT GGATGACATC 
CGCAAACAGA GTGAGCCCTT CTTTAAAGTC ACTCCAGAAG AAAGACCCAA GCTAGAGGAG 
TTCTTTGCCC GTAACTCCTA TGTAGCTGGC CAGTATGATG ATCCAGCCTC CTACAAGCAC 
CTCAACAGCC ACATGAATGC CCTGCACCAG GGAATGCAGG CCAACCGTCT GTTCTACCTG 
GCCTTGCCCC CCACTGTCTA TGAAGCAGTC ACCAAGAACA TTCAAGAGAT CTGCATGAGT 
CAGACAGGCT GGAACCGCAT CATAGTGGAG AAGCCCTTCG GGAGAGACCT GCAGAGCTCC 
AATCAACTGT CGAACCACAT CTCCTCTCTG TTTCGTGAGG ACCAGATCTA CCGCATTGAC 
CACTACCTGG GCAAAGAGAT GGTCCAGAAC CTCATGGTGC TGAGATTTGC CAACAGGATC 
TTTGGACCCA TCTGGAATCG AGACAACATT GCTTGTGTGA TCCTTACATT TAAAGAGCCC 
TTTGGTACTG AGGGTCGTGG GGGCTATTTT GATGAATTTG GGATCATCAG GGATGTCATG 
CAGAACCACC TCCTGCAGAT GTTGTGTCTA GTGGCCATGG AAAAGCCTGC CTCTACAGAT 
TCAGATGATG TCCGTGATGA GAAGGTCAAA GTGTTAAAAT GTATCTCAGA GGTGGAAACT 
GACAACGTGG TCCTTGGCCA GTATGTGGGG AACCCCAGTG GAGAAGGAGA AGCTACCAAT 
GGGTACTTAG ATGACCCCAC AGTACCCCAT GGGTCTACCA CTGCTACCTT TGCAGCAGCT 
GTCCTCTATG TGGAGAATGA ACGGTGGGAT GGAGTACCCT TCATCCTGCG CTGTGGCAAA 
GCTCTGAATG AGCGCAAAGC TGAAGTGAGA CTTCAGTTCC GCGATGTGGC AGGTGACATC 
TTCCACCAGC AGTGCAAGCG TAACGAGCTG GTCATCCGTG TGCAGCCCAA TGAGGCGGTA 
TACACCAAGA TGATGACCAA GAAGCCTGGC ATGTTCTTCA ACCCTGAGGA GTCTGAGCTG 
GACCTAACCT ATGGCAACAG ATACAAGAAT GTGAAGCTCC CTGATGCCTA TGAACGCCTC 
ATCCTGGATG TCTTCTGTGG GAGCCAAATG CACTTTGTCC GTAGTGATGA ACTCAGGGAA 
GCCTGGCGTA TCTTCACACC ATTGCTGCAC AAGATTGATC GAGAGAAGCC CCAGCCCATC 
CCGTATGTCT ATGGCAGCCG AGGTCCCACA GAGGCAGATG AGCTGATGAA GAGAGTGGGC 
TTCCAGTATG AGGGTACCTA CAAGTGGGTG AACCCTCACA AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Purification of glucose-6-phosphate dehydrogenase from rat (Rattus norvegicus) erythrocytes and inhibition effects of some metal ions on enzyme activity.
Journal of biochemical and molecular toxicology31(9)(2017 Sep)
Temel Y,Kocyigit UM

20-HETE-induced mitochondrial superoxide production and inflammatory phenotype in vascular smooth muscle is prevented by glucose-6-phosphate dehydrogenase inhibition.
American journal of physiology. Heart and circulatory physiology310(9)H1107-17(2016 May)
Lakhkar A,Dhagia V,Joshi SR,Gotlinger K,Patel D,Sun D,Wolin MS,Schwartzman ML,Gupte SA

G6PD deficiency and absence of ?-thalassemia increase the risk for cerebral vasculopathy in children with sickle cell anemia.
European journal of haematology96(4)404-8(2016 Apr)
Joly P,Garnier N,Kebaili K,Renoux C,Dony A,Cheikh N,Renard C,Ceraulo A,Cuzzubbo D,Pondarr? C,Martin C,Pialoux V,Francina A,Bertrand Y,Connes P

Hypoxia-induced glucose-6-phosphate dehydrogenase overexpression and -activation in pulmonary artery smooth muscle cells: implication in pulmonary hypertension.
American journal of physiology. Lung cellular and molecular physiology308(3)L287-300(2015 Feb)
Chettimada S,Gupte R,Rawat D,Gebb SA,McMurtry IF,Gupte SA

DNA sequence abnormalities of human glucose-6-phosphate dehydrogenase variants.
The Journal of biological chemistry266(7)4145-50(1991 Mar)
Beutler E,Kuhl W,Gelbart T,Forman L

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G6pd cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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