Cbs cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cbs
Entrez Gene ID	24250
Full Name	cystathionine beta synthase
General protein information	Preferred Names cystathionine beta synthase Names cystathionine beta-synthase beta-thionase hemoprotein H-450 serine sulfhydrase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20p12
Summary	catalyzes condensation of serine and homocyteine to cystathione; involved in transsulfuration pathway; regulated by insulin; alternative splicing produces four mRNA species [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012522.2	NP_036654.2	cystathionine beta-synthase

Pathways from BioSystems

Homologies

Homo sapiens (human)	LOC102724560	XP_006723989.1
Neurospora crassa	NCU08216	XP_959306.1
Oryza sativa (rice)	Os06g0149700	NP_001056819.1
Xenopus tropicalis (tropical clawed frog)	cbs	NP_001008171.1
Homo sapiens (human)	CBS	NP_000062.1
Bos taurus (cattle)	CBS	NP_001095470.1
Mus musculus (house mouse)	Cbs	NP_001258282.1
Rattus norvegicus (Norway rat)	Cbs	NP_036654.2
Gallus gallus (chicken)	CBS	XP_416752.3
Drosophila melanogaster (fruit fly)	Cbs	NP_728384.1
Eremothecium gossypii	AGOS_AGR012C	NP_986677.2
Magnaporthe oryzae (rice blast fungus)	MGG_07384	XP_003711260.1
Canis lupus familiaris (dog)	CBS	XP_853796.2
Danio rerio (zebrafish)	cbsb	NP_001014367.2
Saccharomyces cerevisiae (baker's yeast)	CYS4	NP_011671.3
Kluyveromyces lactis	KLLA0F09317g	XP_455504.1
Pan troglodytes (chimpanzee)	CBS	XP_001143121.1
Danio rerio (zebrafish)	cbsa	NP_001104702.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000162	XP_310977.5
Oryza sativa (rice)	Os06g0564700	NP_001057885.1
Oryza sativa (rice)	Os06g0564500	NP_001057884.1
Oryza sativa (rice)	Os06g0149900	NP_001056820.1

Gene Ontology
Bibliography

Related articles in PubMed

Nitration-mediated deficiency of cystathionine ?-synthase activity accelerates the progression of hyperhomocysteinemia.
Wang H, Sun Q, Zhou Y, Zhang H, Luo C, Xu J, Dong Y, Wu Y, Liu H, Wang W
Free radical biology & medicine113519-529(2017 Dec)

Excessive nNOS/NO/AMPK signaling activation mediated by the blockage of the CBS/H2S system contributes to oxygen?glucose deprivation?induced endoplasmic reticulum stress in PC12?cells.
Zhang R, Lin YQ, Wang WS, Wang XQ
International journal of molecular medicine40(2)549-557(2017 Aug)

Loss of the Protein Cystathionine ?-Synthase During Kidney Injury Promotes Renal Tubulointerstitial Fibrosis.
Yuan X, Zhang J, Xie F, Tan W, Wang S, Huang L, Tao L, Xing Q, Yuan Q
Kidney & blood pressure research42(3)428-443(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We showed that astrocytes in Stroke-prone spontaneously hypertensive rats vulnerable to oxidative stress may be caused by reduction of H2S through lower expression and activity of CBS
Title: Reduced Production of Hydrogen Sulfide and Sulfane Sulfur Due to Low Cystathionine ?-Synthase Levels in Brain Astrocytes of Stroke-Prone Spontaneously Hypertensive Rats.

These results suggest that the lower mRNA expression of cystathionine beta-synthase in the cerebellum and the smaller size of cerebellum may be related to the depression-like behavior in Wistar Kyoto rats.
Title: Suppressed expression of cystathionine ?-synthase and smaller cerebellum in Wistar Kyoto rats.

Results indicate that neurons are essential for maintaining the expression and hydrogen sulfide-producing activity of astrocytic Cystathionine beta-synthase in the rat spinal cord.
Title: Neuronal regulation of expression of hydrogen sulfide-producing enzyme cystathionine ?-synthase in rat spinal cord astrocytes.

Activation of TLR4 inflammatory bowel disease upregulates CBS expression, which is mediated by the NF-kappaB signaling pathway, contributing to visceral hypersensitivity.
Title: TLR4 upregulates CBS expression through NF-?B activation in a rat model of irritable bowel syndrome with chronic visceral hypersensitivity.

Results imply that in acute ischemic conditions, CBS is upregulated and activated by truncation causing an increased production of hydrogen sulfide, which exacerbate the ischemic injuries
Title: Cystathionine ?-synthase inhibition is a potential therapeutic approach to treatment of ischemic injury.

The following Cbs gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cbs cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12746	NM_012522.2 Latest version!	Rattus norvegicus cystathionine beta synthase (Cbs), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cbs ( NM_012522.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036654.2 Rattus norvegicus cystathionine beta synthase (Cbs), mRNA.

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CloneID	ORa12746
Clone ID Related Accession (Same CDS sequence)	NM_012522.2
Accession Version	NM_012522.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	cystathionine beta-synthase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from M88344.1, D01098.1, M88346.1, M88345.1 and AA997754.1. On Oct 12, 2007 this sequence version replaced NM_012522.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D01098.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCCTTCAG GGACATCCCA GTGTGAAGAT GGCTCTGCAG GGTGCCCCCA GGACTTGGAG 
GTACAGCCAG AAAAAGGGCA ACTGGAGAAG GGAGCCTCAG GGGACAAGGA AAGAGTCTGG 
ATCTCGCCTG ATACCCCAAG CAGATGTACT TGGCAGCTGG GCAGGCCCAT GGCGGATTCC 
CCACATTACC ACACAGTGCC GACAAAATCC CCGAAAATTT TGCCAGATAT TCTGAGGAAA 
ATTGGCAACA CCCCTATGGT CAGAATCAAC AGGATCTCCA AGAATGCAGG ACTCAAGTGC 
GAGCTGTTGG CCAAGTGTGA GTTCTTCAAC GCCGGTGGGA GTGTGAAGGA CCGCATCAGC 
CTCCGGATGA TTGAAGACGC TGAGCGAGCC GGAACCTTGA AGCCCGGAGA CACGATCATT 
GAGCCAACTT CTGGCAACAC AGGGATCGGG CTGGCTCTGG CAGCTGCTGT GAAGGGCTAT 
CGCTGCATTA TCGTGATGCC TGAGAAGATG AGTATGGAGA AGGTGGATGT GCTGCGAGCT 
CTGGGAGCTG AGATTGTGAG GACGCCCACC AACGCCAGAT TCGATTCCCC CGAGTCCCAC 
GTAGGAGTGG CATGGCGACT GAAGAACGAA ATCCCCAATT CTCACATTCT GGACCAGTAC 
CGCAATGCCA GCAACCCCTT GGCGCACTAC GATGACACCG CAGAGGAGAT CCTGCAGCAG 
TGCGACGGGA AGGTGGACAT GCTGGTGGCT TCAGCAGGCA CGGGTGGCAC CATCACGGGT 
ATCGCGAGGA AGCTGAAGGA GAAGTGCCCA GGTTGTAAAA TCATCGGTGT AGATCCCGAG 
GGGTCCATCC TCGCGGAGCC CGAGGAGCTG AACCAGACGG AGCAAACAGC CTATGAGGTG 
GAAGGGATCG GCTACGACTT CATCCCCACC GTCCTGGACA GGGCGGTGGT GGATAGGTGG 
TTCAAGAGCA ATGATGACGA TTCCTTCGCC TTCGCCCGCA TGCTCATCTC CCAGGAGGGA 
CTGCTGTGCG GTGGGAGTTC AGGCAGCGCT ATGGCCGTGG CTGTGAAGGC TGCCCAGGAG 
CTAAAGGAAG GACAGCGCTG TGTGGTCATC CTGCCCGACT CTGTGCGCAA CTACATGTCC 
AAGTTCTTGA GTGACAAATG GATGCTGCAG AAAGGCTTCA TGAAGGAGGA GCTCTCCGTG 
AAGAGACCCT GGTGGTGGCA TCTGCGTGTC CAAGAGCTGA GCCTATCAGC ACCGCTGACC 
GTGTTGCCCA CTGTCACCTG TGAGCACACC ATCGCCATCC TCCGGGAGAA GGGTTTTGAC 
CAGGCACCTG TGGTCAACGA GTCTGGGGCC ATCCTAGGGA TGGTGACTCT CGGGAACATG 
TTGTCCTCCC TGCTTGCTGG GAAGGTGCGG CCATCAGACG AAGTCTGCAA AGTCCTCTAC 
AAGCAGTTCA AGCCGATCCA CCTGACCGAC ACACTGGGCA TGCTCTCCCA CATCCTGGAG 
ATGGACCACT TCGCCCTGGT GGTCCATGAG CAGATCCAAT ACCGCAACAA TGGCGTGTCC 
AGCAAGCAGC TGATGGTGTT TGGTGTTGTC ACCGCCATTG ACCTGCTAAA CTTCGTGGCA 
GCCCGTGAGC AGACCCGGAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036654.2
CDS	145..1788
Translation	MPSGTSQCEDGSAGCPQDLEVQPEKGQLEKGASGDKERVWISPDTPSRCTWQLGRPMADSPHYHTVPTKSPKILPDILRKIGNTPMVRINRISKNAGLKCELLAKCEFFNAGGSVKDRISLRMIEDAERAGTLKPGDTIIEPTSGNTGIGLALAAAVKGYRCIIVMPEKMSMEKVDVLRALGAEIVRTPTNARFDSPESHVGVAWRLKNEIPNSHILDQYRNASNPLAHYDDTAEEILQQCDGKVDMLVASAGTGGTITGIARKLKEKCPGCKIIGVDPEGSILAEPEELNQTEQTAYEVEGIGYDFIPTVLDRAVVDRWFKSNDDDSFAFARMLISQEGLLCGGSSGSAMAVAVKAAQELKEGQRCVVILPDSVRNYMSKFLSDKWMLQKGFMKEELSVKRPWWWHLRVQELSLSAPLTVLPTVTCEHTIAILREKGFDQAPVVNESGAILGMVTLGNMLSSLLAGKVRPSDEVCKVLYKQFKPIHLTDTLGMLSHILEMDHFALVVHEQIQYRNNGVSSKQLMVFGVVTAIDLLNFVAAREQTRK

Target ORF information:

RefSeq Version	NM_012522.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cystathionine beta synthase (Cbs), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012522.2

ORF Insert Sequence:

ATGCCTTCAG GGACATCCCA GTGTGAAGAT GGCTCTGCAG GGTGCCCCCA GGACTTGGAG 
GTACAGCCAG AAAAAGGGCA ACTGGAGAAG GGAGCCTCAG GGGACAAGGA AAGAGTCTGG 
ATCTCGCCTG ATACCCCAAG CAGATGTACT TGGCAGCTGG GCAGGCCCAT GGCGGATTCC 
CCACATTACC ACACAGTGCC GACAAAATCC CCGAAAATTT TGCCAGATAT TCTGAGGAAA 
ATTGGCAACA CCCCTATGGT CAGAATCAAC AGGATCTCCA AGAATGCAGG ACTCAAGTGC 
GAGCTGTTGG CCAAGTGTGA GTTCTTCAAC GCCGGTGGGA GTGTGAAGGA CCGCATCAGC 
CTCCGGATGA TTGAAGACGC TGAGCGAGCC GGAACCTTGA AGCCCGGAGA CACGATCATT 
GAGCCAACTT CTGGCAACAC AGGGATCGGG CTGGCTCTGG CAGCTGCTGT GAAGGGCTAT 
CGCTGCATTA TCGTGATGCC TGAGAAGATG AGTATGGAGA AGGTGGATGT GCTGCGAGCT 
CTGGGAGCTG AGATTGTGAG GACGCCCACC AACGCCAGAT TCGATTCCCC CGAGTCCCAC 
GTAGGAGTGG CATGGCGACT GAAGAACGAA ATCCCCAATT CTCACATTCT GGACCAGTAC 
CGCAATGCCA GCAACCCCTT GGCGCACTAC GATGACACCG CAGAGGAGAT CCTGCAGCAG 
TGCGACGGGA AGGTGGACAT GCTGGTGGCT TCAGCAGGCA CGGGTGGCAC CATCACGGGT 
ATCGCGAGGA AGCTGAAGGA GAAGTGCCCA GGTTGTAAAA TCATCGGTGT AGATCCCGAG 
GGGTCCATCC TCGCGGAGCC CGAGGAGCTG AACCAGACGG AGCAAACAGC CTATGAGGTG 
GAAGGGATCG GCTACGACTT CATCCCCACC GTCCTGGACA GGGCGGTGGT GGATAGGTGG 
TTCAAGAGCA ATGATGACGA TTCCTTCGCC TTCGCCCGCA TGCTCATCTC CCAGGAGGGA 
CTGCTGTGCG GTGGGAGTTC AGGCAGCGCT ATGGCCGTGG CTGTGAAGGC TGCCCAGGAG 
CTAAAGGAAG GACAGCGCTG TGTGGTCATC CTGCCCGACT CTGTGCGCAA CTACATGTCC 
AAGTTCTTGA GTGACAAATG GATGCTGCAG AAAGGCTTCA TGAAGGAGGA GCTCTCCGTG 
AAGAGACCCT GGTGGTGGCA TCTGCGTGTC CAAGAGCTGA GCCTATCAGC ACCGCTGACC 
GTGTTGCCCA CTGTCACCTG TGAGCACACC ATCGCCATCC TCCGGGAGAA GGGTTTTGAC 
CAGGCACCTG TGGTCAACGA GTCTGGGGCC ATCCTAGGGA TGGTGACTCT CGGGAACATG 
TTGTCCTCCC TGCTTGCTGG GAAGGTGCGG CCATCAGACG AAGTCTGCAA AGTCCTCTAC 
AAGCAGTTCA AGCCGATCCA CCTGACCGAC ACACTGGGCA TGCTCTCCCA CATCCTGGAG 
ATGGACCACT TCGCCCTGGT GGTCCATGAG CAGATCCAAT ACCGCAACAA TGGCGTGTCC 
AGCAAGCAGC TGATGGTGTT TGGTGTTGTC ACCGCCATTG ACCTGCTAAA CTTCGTGGCA 
GCCCGTGAGC AGACCCGGAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nitration-mediated deficiency of cystathionine ?-synthase activity accelerates the progression of hyperhomocysteinemia.
Free radical biology & medicine113519-529(2017 Dec)
Wang H,Sun Q,Zhou Y,Zhang H,Luo C,Xu J,Dong Y,Wu Y,Liu H,Wang W

Excessive nNOS/NO/AMPK signaling activation mediated by the blockage of the CBS/H2S system contributes to oxygen?glucose deprivation?induced endoplasmic reticulum stress in PC12?cells.
International journal of molecular medicine40(2)549-557(2017 Aug)
Zhang R,Lin YQ,Wang WS,Wang XQ

Loss of the Protein Cystathionine ?-Synthase During Kidney Injury Promotes Renal Tubulointerstitial Fibrosis.
Kidney & blood pressure research42(3)428-443(2017)
Yuan X,Zhang J,Xie F,Tan W,Wang S,Huang L,Tao L,Xing Q,Yuan Q

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Cbs cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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