Aldob cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Aldob
Entrez Gene ID	24190
Full Name	aldolase, fructose-bisphosphate B
Synonyms	Aldo2,LIV10
General protein information	Preferred Names aldolase, fructose-bisphosphate B Names fructose-bisphosphate aldolase B Aldolase B, fructose-biphosphate aldolase B, fructose-bisphosphate liver-type aldolase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q22
Summary	member of the aldolase enzyme family [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012496.2	NP_036628.2	fructose-bisphosphate aldolase B

Pathways from BioSystems

Homologies

Homo sapiens (human)	ALDOB	NP_000026.2
Gallus gallus (chicken)	ALDOB	NP_001007978.1
Canis lupus familiaris (dog)	ALDOB	XP_867333.3
Bos taurus (cattle)	ALDOB	NP_001029657.1
Mus musculus (house mouse)	Aldob	NP_659152.1
Rattus norvegicus (Norway rat)	Aldob	NP_036628.2
Danio rerio (zebrafish)	aldob	NP_919348.3
	ALDOB	XP_001111095.1
Pan troglodytes (chimpanzee)	ALDOB	XP_001136159.1
Xenopus tropicalis (tropical clawed frog)	aldob	NP_989131.1

Gene Ontology
Bibliography

Related articles in PubMed

Type of supplemented simple sugar, not merely calorie intake, determines adverse effects on metabolism and aortic function in female rats.
Sang?esa G, Shaligram S, Akther F, Roglans N, Laguna JC, Rahimian R, Alegret M
American journal of physiology. Heart and circulatory physiology312(2)H289-H304(2017 Feb)

A new level of regulation in gluconeogenesis: metabolic state modulates the intracellular localization of aldolase B and its interaction with liver fructose-1,6-bisphosphatase.
Droppelmann CA, S?ez DE, Asenjo JL, Y??ez AJ, Garc?a-Rocha M, Concha II, Grez M, Guinovart JJ, Slebe JC
The Biochemical journal472(2)225-37(2015 Dec)

Aldolase-B knockout in mice phenocopies hereditary fructose intolerance in humans.
Oppelt SA, Sennott EM, Tolan DR
Molecular genetics and metabolism114(3)445-50(2015 Mar)

Upregulation of aldolase B and overproduction of methylglyoxal in vascular tissues from rats with metabolic syndrome.
Liu J, Wang R, Desai K, Wu L
Cardiovascular research92(3)494-503(2011 Dec)

The heme oxygenase system abates hyperglycemia in Zucker diabetic fatty rats by potentiating insulin-sensitizing pathways.
Ndisang JF, Lane N, Jadhav A
Endocrinology150(5)2098-108(2009 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest energy metabolism (gluconeogenesis versus glycolysis) modulates aldolase B and fructose-1,6-bisphosphatase-1 activities in hepatocytes via their interaction suggesting their association confers catalytic advantage for both enzymes.
Title: A new level of regulation in gluconeogenesis: metabolic state modulates the intracellular localization of aldolase B and its interaction with liver fructose-1,6-bisphosphatase.

Upregulation of aldolase B by accumulated fructose is a common mechanism for methylglyoxal overproduction in vascular smooth muscle cella and aorta in different models of metabolic syndrome.
Title: Upregulation of aldolase B and overproduction of methylglyoxal in vascular tissues from rats with metabolic syndrome.

Binding of ALF-C enhances replicator activity of Aldob.
Title: Binding of AlF-C, an Orc1-binding transcriptional regulator, enhances replicator activity of the rat aldolase B origin.

Data indicate the presence of a modulated interaction between fructose-1,6-bisphosphatase and aldolase B, and suggest that in kidney, aldolase B specifically participates in gluconeogenesis and with aldolase A in glycolysis.
Title: Different involvement for aldolase isoenzymes in kidney glucose metabolism: aldolase B but not aldolase A colocalizes and forms a complex with FBPase.

The following Aldob gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Aldob cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00082	NM_012496.2 Latest version!	Rattus norvegicus aldolase, fructose-bisphosphate B (Aldob), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Aldob ( NM_012496.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036628.2 Rattus norvegicus aldolase, fructose-bisphosphate B (Aldob), mRNA.

Price & Availability↑

CloneID	ORa00082
Clone ID Related Accession (Same CDS sequence)	NM_012496.2
Accession Version	NM_012496.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Rattus norvegicus(Norway rat)
Product	fructose-bisphosphate aldolase B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC081697.1. On Oct 10, 2007 this sequence version replaced NM_012496.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC081697.1, FQ218551.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906351, SAMN01906352 [ECO:0000348] ##Evidence-Data-END##

ATGGCTCACC GATTTCCAGC CCTCACCTCA GAGCAGAAGA AGGAGCTCTC CGAGATTGCG 
CAGCGCATTG TTGCCAATGG GAAGGGTATC CTGGCTGCAG ATGAGTCTGT GGGCACCATG 
GGAAACCGCC TACAGAGGAT AAAGGTGGAA AACACTGAAG AGAACCGAAG GCAGTTCCGA 
GAGCTCCTCT TTAGTGTGGA CAATTCTATC AGCCAGAGCA TCGGCGGAGT GATCCTTTTC 
CATGAGACGC TCTACCAGAA GGACAGCCAG GGAAAGCTGT TCAGAAACAT TCTCAAGGAG 
AAGGGAATTG TGGTGGGCAT CAAGCTGGAC CAAGGAGGTG CCCCGCTTGC AGGAACAAAC 
AAAGAAACCA CCATTCAAGG GCTTGACGGC CTCTCCGAAC GCTGTGCTCA GTACAAGAAA 
GACGGAGTCG ACTTTGGGAA GTGGCGTGCT GTGCTGAGGA TCTCGGACCA GTGCCCTTCC 
AGCCTTGCTA TCCAAGAAAA CGCCAACGCT CTGGCTCGCT ACGCCAGCAT CTGCCAGCAG 
AATGGGCTGG TACCTATTGT TGAGCCAGAG GTTCTTCCTG ATGGAGACCA TGACCTAGAG 
CACTGCCAGT ATGTTTCTGA GAAGGTCCTG GCTGCTGTCT ACAAGGCTCT CAATGATCAT 
CATGTTTACC TTGAGGGCAC CCTGCTAAAG CCAAACATGG TGACTGCTGG ACATGCCTGC 
ACCAAGAAGT ACACACCTGA GCAAGTGGCT ATGGCCACCG TCACGGCTCT CCACAGAACT 
GTTCCTGCAG CTGTGCCTGG TATCTGCTTT TTGTCTGGAG GCATGAGTGA GGAGGATGCT 
ACACTTAACC TCAATGCTAT CAACCGTTGC CCTCTACCTA GGCCCTGGAA ACTAAGCTTT 
TCATACGGCA GAGCCCTCCA GGCCAGTGCA TTGGCTGCCT GGGGCGGCAA GGCTGCAAAC 
AAGAAGGCAA CCCAGGAAGC TTTCATGAAG CGGGCTGTGG CCAACTGCCA GGCAGCCCAA 
GGACAGTATG TTCACACGGG CTCGTCAGGC GCTGCTTCCA CGCAGTCACT CTTCACAGCC 
TCCTACACCT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036628.2
CDS	58..1152
Translation	MAHRFPALTSEQKKELSEIAQRIVANGKGILAADESVGTMGNRLQRIKVENTEENRRQFRELLFSVDNSISQSIGGVILFHETLYQKDSQGKLFRNILKEKGIVVGIKLDQGGAPLAGTNKETTIQGLDGLSERCAQYKKDGVDFGKWRAVLRISDQCPSSLAIQENANALARYASICQQNGLVPIVEPEVLPDGDHDLEHCQYVSEKVLAAVYKALNDHHVYLEGTLLKPNMVTAGHACTKKYTPEQVAMATVTALHRTVPAAVPGICFLSGGMSEEDATLNLNAINRCPLPRPWKLSFSYGRALQASALAAWGGKAANKKATQEAFMKRAVANCQAAQGQYVHTGSSGAASTQSLFTASYTY

Target ORF information:

RefSeq Version	NM_012496.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus aldolase, fructose-bisphosphate B (Aldob), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012496.2

ORF Insert Sequence:

ATGGCTCACC GATTTCCAGC CCTCACCTCA GAGCAGAAGA AGGAGCTCTC CGAGATTGCG 
CAGCGCATTG TTGCCAATGG GAAGGGTATC CTGGCTGCAG ATGAGTCTGT GGGCACCATG 
GGAAACCGCC TACAGAGGAT AAAGGTGGAA AACACTGAAG AGAACCGAAG GCAGTTCCGA 
GAGCTCCTCT TTAGTGTGGA CAATTCTATC AGCCAGAGCA TCGGCGGAGT GATCCTTTTC 
CATGAGACGC TCTACCAGAA GGACAGCCAG GGAAAGCTGT TCAGAAACAT TCTCAAGGAG 
AAGGGAATTG TGGTGGGCAT CAAGCTGGAC CAAGGAGGTG CCCCGCTTGC AGGAACAAAC 
AAAGAAACCA CCATTCAAGG GCTTGACGGC CTCTCCGAAC GCTGTGCTCA GTACAAGAAA 
GACGGAGTCG ACTTTGGGAA GTGGCGTGCT GTGCTGAGGA TCTCGGACCA GTGCCCTTCC 
AGCCTTGCTA TCCAAGAAAA CGCCAACGCT CTGGCTCGCT ACGCCAGCAT CTGCCAGCAG 
AATGGGCTGG TACCTATTGT TGAGCCAGAG GTTCTTCCTG ATGGAGACCA TGACCTAGAG 
CACTGCCAGT ATGTTTCTGA GAAGGTCCTG GCTGCTGTCT ACAAGGCTCT CAATGATCAT 
CATGTTTACC TTGAGGGCAC CCTGCTAAAG CCAAACATGG TGACTGCTGG ACATGCCTGC 
ACCAAGAAGT ACACACCTGA GCAAGTGGCT ATGGCCACCG TCACGGCTCT CCACAGAACT 
GTTCCTGCAG CTGTGCCTGG TATCTGCTTT TTGTCTGGAG GCATGAGTGA GGAGGATGCT 
ACACTTAACC TCAATGCTAT CAACCGTTGC CCTCTACCTA GGCCCTGGAA ACTAAGCTTT 
TCATACGGCA GAGCCCTCCA GGCCAGTGCA TTGGCTGCCT GGGGCGGCAA GGCTGCAAAC 
AAGAAGGCAA CCCAGGAAGC TTTCATGAAG CGGGCTGTGG CCAACTGCCA GGCAGCCCAA 
GGACAGTATG TTCACACGGG CTCGTCAGGC GCTGCTTCCA CGCAGTCACT CTTCACAGCC 
TCCTACACCT ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Type of supplemented simple sugar, not merely calorie intake, determines adverse effects on metabolism and aortic function in female rats.
American journal of physiology. Heart and circulatory physiology312(2)H289-H304(2017 Feb)
Sang?esa G,Shaligram S,Akther F,Roglans N,Laguna JC,Rahimian R,Alegret M

A new level of regulation in gluconeogenesis: metabolic state modulates the intracellular localization of aldolase B and its interaction with liver fructose-1,6-bisphosphatase.
The Biochemical journal472(2)225-37(2015 Dec)
Droppelmann CA,S?ez DE,Asenjo JL,Y??ez AJ,Garc?a-Rocha M,Concha II,Grez M,Guinovart JJ,Slebe JC

Aldolase-B knockout in mice phenocopies hereditary fructose intolerance in humans.
Molecular genetics and metabolism114(3)445-50(2015 Mar)
Oppelt SA,Sennott EM,Tolan DR

Upregulation of aldolase B and overproduction of methylglyoxal in vascular tissues from rats with metabolic syndrome.
Cardiovascular research92(3)494-503(2011 Dec)
Liu J,Wang R,Desai K,Wu L

The heme oxygenase system abates hyperglycemia in Zucker diabetic fatty rats by potentiating insulin-sensitizing pathways.
Endocrinology150(5)2098-108(2009 May)
Ndisang JF,Lane N,Jadhav A

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Aldob cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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