Pde9a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pde9a
Entrez Gene ID	191569
Full Name	phosphodiesterase 9A
General protein information	Preferred Names phosphodiesterase 9A Names high affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20p12
Summary	mediates maintenance of low cGMP levels in cells; may play a role in cGMP-mediated signal transduction [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017601537.1	XP_017457026.1	high affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A isoform X2
XM_008772794.2	XP_008771016.1	high affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A isoform X1
NM_138543.1	NP_612552.1	high affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A
XM_008772795.2	XP_008771017.1	high affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A isoform X1
XM_008772793.2	XP_008771015.1	high affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pde9a	NP_032830.3
Homo sapiens (human)	PDE9A	NP_001001567.1
Canis lupus familiaris (dog)	PDE9A	XP_853727.2
Pan troglodytes (chimpanzee)	PDE9A	XP_001140087.1
	PDE9A2	NP_001154921.1
Danio rerio (zebrafish)	pde9a	XP_003199258.1
Xenopus tropicalis (tropical clawed frog)	LOC100493061	XP_002937564.2
Bos taurus (cattle)	PDE9A	XP_005202092.1
Rattus norvegicus (Norway rat)	Pde9a	NP_612552.1
Gallus gallus (chicken)	PDE9A	XP_416748.3

Gene Ontology
Bibliography

Related articles in PubMed

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Yang X, Coulombe-Huntington J, Kang S, Sheynkman GM, Hao T, Richardson A, Sun S, Yang F, Shen YA, Murray RR, Spirohn K, Begg BE, Duran-Frigola M, MacWilliams A, Pevzner SJ, Zhong Q, Trigg SA, Tam S, Ghamsari L, Sahni N, Yi S, Rodriguez MD, Balcha D, Tan G, Costanzo M, Andrews B, Boone C, Zhou XJ, Salehi-Ashtiani K, Charloteaux B, Chen AA, Calderwood MA, Aloy P, Roth FP, Hill DE, Iakoucheva LM, Xia Y, Vidal M
Cell164(4)805-17(2016 Feb)

Phosphodiesterase 9A controls nitric-oxide-independent cGMP and hypertrophic heart disease.
Lee DI, Zhu G, Sasaki T, Cho GS, Hamdani N, Holewinski R, Jo SH, Danner T, Zhang M, Rainer PP, Bedja D, Kirk JA, Ranek MJ, Dostmann WR, Kwon C, Margulies KB, Van Eyk JE, Paulus WJ, Takimoto E, Kass DA
Nature519(7544)472-6(2015 Mar)

PDE9A inhibition rescues amyloid beta-induced deficits in synaptic plasticity and cognition.
Kroker KS, Mathis C, Marti A, Cassel JC, Rosenbrock H, Dorner-Ciossek C
Neurobiology of aging35(9)2072-8(2014 Sep)

PDE9A, PDE10A, and PDE11A expression in rat trigeminovascular pain signalling system.
Kruse LS, M?ller M, Tibaek M, Gammeltoft S, Olesen J, Kruuse C
Brain research128125-34(2009 Jul)

Cell array-based intracellular localization screening reveals novel functional features of human chromosome 21 proteins.
Hu YH, Warnatz HJ, Vanhecke D, Wagner F, Fiebitz A, Thamm S, Kahlem P, Lehrach H, Yaspo ML, Janitz M
BMC genomics7155(2006 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

It is a cGMP degrading phosphodiesterases, in which, cGMP is an important intracellular messenger for synaptic plasticity and memory function.
Title: PDE9A inhibition rescues amyloid beta-induced deficits in synaptic plasticity and cognition.

We here present, for the first time, the expression of PDE9A, PDE10A, and PDE11A in the trigeminovascular system; localisation indicates that they may have a role in pain pathways
Title: PDE9A, PDE10A, and PDE11A expression in rat trigeminovascular pain signalling system.

The following Pde9a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pde9a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa41977	NM_138543.1 Latest version!	Rattus norvegicus phosphodiesterase 9A (Pde9a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa44355	XM_008772795.2 Latest version!	Rattus norvegicus phosphodiesterase 9A (Pde9a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa44355	XM_008772793.2 Latest version!	Rattus norvegicus phosphodiesterase 9A (Pde9a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa44355	XM_008772794.2 Latest version!	Rattus norvegicus phosphodiesterase 9A (Pde9a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa60034	XM_017601537.1 Latest version!	Rattus norvegicus phosphodiesterase 9A (Pde9a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pde9a ( NM_138543.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_612552.1 Rattus norvegicus phosphodiesterase 9A (Pde9a), mRNA.

Price & Availability↑

CloneID	ORa41977
Clone ID Related Accession (Same CDS sequence)	NM_138543.1
Accession Version	NM_138543.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	high affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF372654.1. ##Evidence-Data-START## Transcript exon combination :: AF372654.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGGGCCG GCTCCTCAAG CTACCGGCCC AAGGCCATCT ACCTGGACAT CGATGGACGC 
ATCCAGAAGG TGGTTTTCAG CAAGTATTGT AACTCCAGTG ACATCATGGA CCTGTTCTGT 
ATCGCCACCG GCCTGCCACG GAATACCACC ATCTCCCTTC TGACCACAGA CGACGCCATG 
GTCTCCATCG ATCCCACCAT GCCTGCGAAT TCAGAGCGAA CTCCCTACAA GGTGAGACCT 
GTGGCTGTCA AGCAAGTGTC TGAAAGAGAA GAACTCGTCC AGGGTGTGCT GGCCCAAGTG 
GCGGAACAAT TCTCCAGAGC GTTTAAGATC AACGAGCTGA AAGCTGAAGT TGCGAATCAC 
CTGGCCATGC TGGAGAAACG GGTGGAATTG GAAGGACTCA AAGTGGTGGA GATCGAAAAA 
TGCAAGAGTG ATATTAAAAA GATGCGGGAG GAGCTGGCAG CTAGAAACAA CAGGACCAAC 
TGTCCCTGTA AATACAGTTT TTTGGATAAC AAGAAGTTGA CACCTCGACG TGATGTCCCC 
ACTTACCCCA AGTACCTGCT CTCCCCAGAA ACCATTGAAG CCCTACGGAA GCCCACCTTT 
GATGTCTGGC TTTGGGAACC CAATGAGATG CTGAGCTGTT TAGAGCATAT GTACCATGAC 
CTTGGTCTGG TCAGGGACTT CAGCATCAAC CCAATCACAC TCCGCAGGTG GCTGCTCTGT 
GTGCATGACA ACTACAGGAG CAACCCCTTC CACAACTTCC GGCACTGCTT CTGTGTGACA 
CAGATGATGT ACAGCATGGT CTGGCTCTGT GGCCTCCAGG AGAAGTTTTC CCAGATGGAC 
ATCTTGGTCC TGATGACCGC AGCCATCTGC CATGACCTGG ACCACCCAGG GTACAACAAC 
ACATACCAGA TCAACGCCCG CACGGAACTA GCGGTGCGCT ACAACGACAT CTCGCCGCTG 
GAGAACCACC ATTGCGCCAT CGCCTTCCAG ATCCTGGCGA GACCCGAGTG CAACATCTTC 
GCCAGCGTGC CGCCTGAGGG CTTCAGGCAG ATCAGGCAGG GGATGATCAC GTTGATCTTG 
GCCACCGACA TGGCAAGGCA CGCTGAAATT ATGGATTCTT TCAAAGAAAA AATGGAGAAT 
TTTGACTACA GCAATGAGGA GCATCTGACC CTGCTGAAGA TGATCCTCAT AAAATGCTGT 
GACATCTCCA ATGAGGTCCG TCCCATGGAG GTGGCAGAAC CGTGGGTGGA CTGTTTACTG 
GAAGAATATT TTATGCAGAG TGACCGCGAG AAGTCCGAAG GCCTTCCTGT GGCCCCATTC 
ATGGACCGAG ACAAAGTGAC CAAAGCGACA GCCCAAATTG GGTTCATCAA GTTTGTCCTG 
ATCCCAATGT TTGAAACAGT GACCAAGCTC TTCCCCATTG TCGAGGAGAC CATGCTGCGG 
CCTCTCTGGG AGTCCCGAGA ACACTACGAG GAACTGAAGC AGCTGGACGA CGCCATGAAG 
GAGCTGCAGA AGAAGACGGA GAACTTAACA TCTGGGGCCA CGGAAAACGC CCCAGAGAAG 
ACCAGAGATG CGAAAGACAA TGAAGACCGT TCGCCTCCAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_612552.1
CDS	43..1647
Translation	MGAGSSSYRPKAIYLDIDGRIQKVVFSKYCNSSDIMDLFCIATGLPRNTTISLLTTDDAMVSIDPTMPANSERTPYKVRPVAVKQVSEREELVQGVLAQVAEQFSRAFKINELKAEVANHLAMLEKRVELEGLKVVEIEKCKSDIKKMREELAARNNRTNCPCKYSFLDNKKLTPRRDVPTYPKYLLSPETIEALRKPTFDVWLWEPNEMLSCLEHMYHDLGLVRDFSINPITLRRWLLCVHDNYRSNPFHNFRHCFCVTQMMYSMVWLCGLQEKFSQMDILVLMTAAICHDLDHPGYNNTYQINARTELAVRYNDISPLENHHCAIAFQILARPECNIFASVPPEGFRQIRQGMITLILATDMARHAEIMDSFKEKMENFDYSNEEHLTLLKMILIKCCDISNEVRPMEVAEPWVDCLLEEYFMQSDREKSEGLPVAPFMDRDKVTKATAQIGFIKFVLIPMFETVTKLFPIVEETMLRPLWESREHYEELKQLDDAMKELQKKTENLTSGATENAPEKTRDAKDNEDRSPPN

Target ORF information:

RefSeq Version	NM_138543.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphodiesterase 9A (Pde9a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138543.1

ORF Insert Sequence:

ATGGGGGCCG GCTCCTCAAG CTACCGGCCC AAGGCCATCT ACCTGGACAT CGATGGACGC 
ATCCAGAAGG TGGTTTTCAG CAAGTATTGT AACTCCAGTG ACATCATGGA CCTGTTCTGT 
ATCGCCACCG GCCTGCCACG GAATACCACC ATCTCCCTTC TGACCACAGA CGACGCCATG 
GTCTCCATCG ATCCCACCAT GCCTGCGAAT TCAGAGCGAA CTCCCTACAA GGTGAGACCT 
GTGGCTGTCA AGCAAGTGTC TGAAAGAGAA GAACTCGTCC AGGGTGTGCT GGCCCAAGTG 
GCGGAACAAT TCTCCAGAGC GTTTAAGATC AACGAGCTGA AAGCTGAAGT TGCGAATCAC 
CTGGCCATGC TGGAGAAACG GGTGGAATTG GAAGGACTCA AAGTGGTGGA GATCGAAAAA 
TGCAAGAGTG ATATTAAAAA GATGCGGGAG GAGCTGGCAG CTAGAAACAA CAGGACCAAC 
TGTCCCTGTA AATACAGTTT TTTGGATAAC AAGAAGTTGA CACCTCGACG TGATGTCCCC 
ACTTACCCCA AGTACCTGCT CTCCCCAGAA ACCATTGAAG CCCTACGGAA GCCCACCTTT 
GATGTCTGGC TTTGGGAACC CAATGAGATG CTGAGCTGTT TAGAGCATAT GTACCATGAC 
CTTGGTCTGG TCAGGGACTT CAGCATCAAC CCAATCACAC TCCGCAGGTG GCTGCTCTGT 
GTGCATGACA ACTACAGGAG CAACCCCTTC CACAACTTCC GGCACTGCTT CTGTGTGACA 
CAGATGATGT ACAGCATGGT CTGGCTCTGT GGCCTCCAGG AGAAGTTTTC CCAGATGGAC 
ATCTTGGTCC TGATGACCGC AGCCATCTGC CATGACCTGG ACCACCCAGG GTACAACAAC 
ACATACCAGA TCAACGCCCG CACGGAACTA GCGGTGCGCT ACAACGACAT CTCGCCGCTG 
GAGAACCACC ATTGCGCCAT CGCCTTCCAG ATCCTGGCGA GACCCGAGTG CAACATCTTC 
GCCAGCGTGC CGCCTGAGGG CTTCAGGCAG ATCAGGCAGG GGATGATCAC GTTGATCTTG 
GCCACCGACA TGGCAAGGCA CGCTGAAATT ATGGATTCTT TCAAAGAAAA AATGGAGAAT 
TTTGACTACA GCAATGAGGA GCATCTGACC CTGCTGAAGA TGATCCTCAT AAAATGCTGT 
GACATCTCCA ATGAGGTCCG TCCCATGGAG GTGGCAGAAC CGTGGGTGGA CTGTTTACTG 
GAAGAATATT TTATGCAGAG TGACCGCGAG AAGTCCGAAG GCCTTCCTGT GGCCCCATTC 
ATGGACCGAG ACAAAGTGAC CAAAGCGACA GCCCAAATTG GGTTCATCAA GTTTGTCCTG 
ATCCCAATGT TTGAAACAGT GACCAAGCTC TTCCCCATTG TCGAGGAGAC CATGCTGCGG 
CCTCTCTGGG AGTCCCGAGA ACACTACGAG GAACTGAAGC AGCTGGACGA CGCCATGAAG 
GAGCTGCAGA AGAAGACGGA GAACTTAACA TCTGGGGCCA CGGAAAACGC CCCAGAGAAG 
ACCAGAGATG CGAAAGACAA TGAAGACCGT TCGCCTCCAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde9a ( XM_008772794.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008771016.1 Rattus norvegicus phosphodiesterase 9A (Pde9a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa44355
Clone ID Related Accession (Same CDS sequence)	XM_008772794.2 , XM_008772795.2 , XM_008772793.2
Accession Version	XM_008772794.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	high affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772794.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCCG GCTCCTCAAG CTACCGGCCC AAGGCCATCT ACCTGGACAT CGATGGACGC 
ATCCAGAAGG TGGTTTTCAG CAAGTATTGT AACTCCAGTG ACATCATGGA CCTGTTCTGT 
ATCGCCACCG GCCTGCCACG GAATACCACC ATCTCCCTTC TGACCACAGA CGACGCCATG 
GTCTCCATCG ATCCCACCAT GCCTGCGAAT TCAGAGCGAA CTCCCTACAA GGTGAGACCT 
GTGGCTGTCA AGCAAGTGTC TGAAAGAGAA GAACTCGTCC AGGGTGTGCT GGCCCAAGTG 
GCGGAACAAT TCTCCAGAGC GTTTAAGATC AACGAGCTGA AAGCTGAAGT TGCGAATCAC 
CTGGCCATGC TGGAGAAACG GGTGGAATTG GAAGGACTCA AAGTGGTGGA GATCGAAAAA 
TGCAAGAGTG ATATTAAAAA GATGCGGGAG GAGCTGGCAG CTAGAAACAA CAGGACCAAC 
TGTCCCTGTA AATACAGTTT TTTGGATAAC AAGAAGTTGA CACCTCGACG TGATGTCCCC 
ACTTACCCCA AGTACCTGCT CTCCCCAGAA ACCATTGAAG CCCTACGGAA GCCCACCTTT 
GATGTCTGGC TTTGGGAACC CAATGAGATG CTGAGCTGTT TAGAGCATAT GTACCATGAC 
CTTGGTCTGG TCAGGGACTT CAGCATCAAC CCAATCACAC TCCGCAGGTG GCTGCTCTGT 
GTGCATGACA ACTACAGGAG CAACCCCTTC CACAACTTCC GGCACTGCTT CTGTGTGACA 
CAGATGATGT ACAGCATGGT CTGGCTCTGT GGCCTCCAGG AGAAGTTTTC CCAGATGGAC 
ATCTTGGTCC TGATGACCGC AGCCATCTGC CATGACCTGG ACCACCCAGG GTACAACAAC 
ACATACCAGA TCAACGCCCG CACGGAACTA GCGGTGCGCT ACAACGACAT CTCGCCGCTG 
GAGAACCACC ATTGCGCCAT CGCCTTCCAG ATCCTGGCGA GACCCGAGTG CAACATCTTC 
GCCAGCGTGC CGCCTGAGGG CTTCAGGCAG ATCAGGCAGG GGATGATCAC GTTGATCTTG 
GCCACCGACA TGGCAAGGCA CGCTGAAATT ATGGATTCTT TCAAAGAAAA AATGGAGAAT 
TTTGACTACA GCAATGAGGA GCATCTGACC CTGCTGAAGA TGATCCTCAT AAAATGCTGT 
GACATCTCCA ATGAGGTCCG TCCCATGGAG GTGGCAGAAC CGTGGGTGGA CTGTTTACTG 
GAAGAATATT TTATGCAGAG TGACCGCGAG AAGTCCGAAG GCCTTCCTGT GGCCCCATTC 
ATGGACCGAG ACAAAGTGAC CAAAGCGACA GCCCAAATTG GGTTCATCAA GTTTGTCCTA 
ATCCCAATGT TTGAAACAGT GACCAAGCTC TTCCCCATTG TCGAGGAGAC CATGCTGCGG 
CCCCTCTGGG AGTCCCGAGA ACACTACGAG GAGCTGAAGC AGCTGGACGA CGCCATGAAG 
GAGCTGCAGA AGAAAACGGA GAACTTAACA TCTGGGGCCA CGGAAAACGC CCCAGAGAAG 
ACCAGAGATG CGAAAGACAA TGAAGACCGT TCACCTCCAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008771016.1
CDS	59..1663
Translation	MGAGSSSYRPKAIYLDIDGRIQKVVFSKYCNSSDIMDLFCIATGLPRNTTISLLTTDDAMVSIDPTMPANSERTPYKVRPVAVKQVSEREELVQGVLAQVAEQFSRAFKINELKAEVANHLAMLEKRVELEGLKVVEIEKCKSDIKKMREELAARNNRTNCPCKYSFLDNKKLTPRRDVPTYPKYLLSPETIEALRKPTFDVWLWEPNEMLSCLEHMYHDLGLVRDFSINPITLRRWLLCVHDNYRSNPFHNFRHCFCVTQMMYSMVWLCGLQEKFSQMDILVLMTAAICHDLDHPGYNNTYQINARTELAVRYNDISPLENHHCAIAFQILARPECNIFASVPPEGFRQIRQGMITLILATDMARHAEIMDSFKEKMENFDYSNEEHLTLLKMILIKCCDISNEVRPMEVAEPWVDCLLEEYFMQSDREKSEGLPVAPFMDRDKVTKATAQIGFIKFVLIPMFETVTKLFPIVEETMLRPLWESREHYEELKQLDDAMKELQKKTENLTSGATENAPEKTRDAKDNEDRSPPN

Target ORF information:

RefSeq Version	XM_008772794.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphodiesterase 9A (Pde9a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772794.2

ORF Insert Sequence:

ATGGGGGCCG GCTCCTCAAG CTACCGGCCC AAGGCCATCT ACCTGGACAT CGATGGACGC 
ATCCAGAAGG TGGTTTTCAG CAAGTATTGT AACTCCAGTG ACATCATGGA CCTGTTCTGT 
ATCGCCACCG GCCTGCCACG GAATACCACC ATCTCCCTTC TGACCACAGA CGACGCCATG 
GTCTCCATCG ATCCCACCAT GCCTGCGAAT TCAGAGCGAA CTCCCTACAA GGTGAGACCT 
GTGGCTGTCA AGCAAGTGTC TGAAAGAGAA GAACTCGTCC AGGGTGTGCT GGCCCAAGTG 
GCGGAACAAT TCTCCAGAGC GTTTAAGATC AACGAGCTGA AAGCTGAAGT TGCGAATCAC 
CTGGCCATGC TGGAGAAACG GGTGGAATTG GAAGGACTCA AAGTGGTGGA GATCGAAAAA 
TGCAAGAGTG ATATTAAAAA GATGCGGGAG GAGCTGGCAG CTAGAAACAA CAGGACCAAC 
TGTCCCTGTA AATACAGTTT TTTGGATAAC AAGAAGTTGA CACCTCGACG TGATGTCCCC 
ACTTACCCCA AGTACCTGCT CTCCCCAGAA ACCATTGAAG CCCTACGGAA GCCCACCTTT 
GATGTCTGGC TTTGGGAACC CAATGAGATG CTGAGCTGTT TAGAGCATAT GTACCATGAC 
CTTGGTCTGG TCAGGGACTT CAGCATCAAC CCAATCACAC TCCGCAGGTG GCTGCTCTGT 
GTGCATGACA ACTACAGGAG CAACCCCTTC CACAACTTCC GGCACTGCTT CTGTGTGACA 
CAGATGATGT ACAGCATGGT CTGGCTCTGT GGCCTCCAGG AGAAGTTTTC CCAGATGGAC 
ATCTTGGTCC TGATGACCGC AGCCATCTGC CATGACCTGG ACCACCCAGG GTACAACAAC 
ACATACCAGA TCAACGCCCG CACGGAACTA GCGGTGCGCT ACAACGACAT CTCGCCGCTG 
GAGAACCACC ATTGCGCCAT CGCCTTCCAG ATCCTGGCGA GACCCGAGTG CAACATCTTC 
GCCAGCGTGC CGCCTGAGGG CTTCAGGCAG ATCAGGCAGG GGATGATCAC GTTGATCTTG 
GCCACCGACA TGGCAAGGCA CGCTGAAATT ATGGATTCTT TCAAAGAAAA AATGGAGAAT 
TTTGACTACA GCAATGAGGA GCATCTGACC CTGCTGAAGA TGATCCTCAT AAAATGCTGT 
GACATCTCCA ATGAGGTCCG TCCCATGGAG GTGGCAGAAC CGTGGGTGGA CTGTTTACTG 
GAAGAATATT TTATGCAGAG TGACCGCGAG AAGTCCGAAG GCCTTCCTGT GGCCCCATTC 
ATGGACCGAG ACAAAGTGAC CAAAGCGACA GCCCAAATTG GGTTCATCAA GTTTGTCCTA 
ATCCCAATGT TTGAAACAGT GACCAAGCTC TTCCCCATTG TCGAGGAGAC CATGCTGCGG 
CCCCTCTGGG AGTCCCGAGA ACACTACGAG GAGCTGAAGC AGCTGGACGA CGCCATGAAG 
GAGCTGCAGA AGAAAACGGA GAACTTAACA TCTGGGGCCA CGGAAAACGC CCCAGAGAAG 
ACCAGAGATG CGAAAGACAA TGAAGACCGT TCACCTCCAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde9a ( XM_008772795.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008771017.1 Rattus norvegicus phosphodiesterase 9A (Pde9a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa44355
Clone ID Related Accession (Same CDS sequence)	XM_008772794.2 , XM_008772795.2 , XM_008772793.2
Accession Version	XM_008772795.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	high affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772795.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCCG GCTCCTCAAG CTACCGGCCC AAGGCCATCT ACCTGGACAT CGATGGACGC 
ATCCAGAAGG TGGTTTTCAG CAAGTATTGT AACTCCAGTG ACATCATGGA CCTGTTCTGT 
ATCGCCACCG GCCTGCCACG GAATACCACC ATCTCCCTTC TGACCACAGA CGACGCCATG 
GTCTCCATCG ATCCCACCAT GCCTGCGAAT TCAGAGCGAA CTCCCTACAA GGTGAGACCT 
GTGGCTGTCA AGCAAGTGTC TGAAAGAGAA GAACTCGTCC AGGGTGTGCT GGCCCAAGTG 
GCGGAACAAT TCTCCAGAGC GTTTAAGATC AACGAGCTGA AAGCTGAAGT TGCGAATCAC 
CTGGCCATGC TGGAGAAACG GGTGGAATTG GAAGGACTCA AAGTGGTGGA GATCGAAAAA 
TGCAAGAGTG ATATTAAAAA GATGCGGGAG GAGCTGGCAG CTAGAAACAA CAGGACCAAC 
TGTCCCTGTA AATACAGTTT TTTGGATAAC AAGAAGTTGA CACCTCGACG TGATGTCCCC 
ACTTACCCCA AGTACCTGCT CTCCCCAGAA ACCATTGAAG CCCTACGGAA GCCCACCTTT 
GATGTCTGGC TTTGGGAACC CAATGAGATG CTGAGCTGTT TAGAGCATAT GTACCATGAC 
CTTGGTCTGG TCAGGGACTT CAGCATCAAC CCAATCACAC TCCGCAGGTG GCTGCTCTGT 
GTGCATGACA ACTACAGGAG CAACCCCTTC CACAACTTCC GGCACTGCTT CTGTGTGACA 
CAGATGATGT ACAGCATGGT CTGGCTCTGT GGCCTCCAGG AGAAGTTTTC CCAGATGGAC 
ATCTTGGTCC TGATGACCGC AGCCATCTGC CATGACCTGG ACCACCCAGG GTACAACAAC 
ACATACCAGA TCAACGCCCG CACGGAACTA GCGGTGCGCT ACAACGACAT CTCGCCGCTG 
GAGAACCACC ATTGCGCCAT CGCCTTCCAG ATCCTGGCGA GACCCGAGTG CAACATCTTC 
GCCAGCGTGC CGCCTGAGGG CTTCAGGCAG ATCAGGCAGG GGATGATCAC GTTGATCTTG 
GCCACCGACA TGGCAAGGCA CGCTGAAATT ATGGATTCTT TCAAAGAAAA AATGGAGAAT 
TTTGACTACA GCAATGAGGA GCATCTGACC CTGCTGAAGA TGATCCTCAT AAAATGCTGT 
GACATCTCCA ATGAGGTCCG TCCCATGGAG GTGGCAGAAC CGTGGGTGGA CTGTTTACTG 
GAAGAATATT TTATGCAGAG TGACCGCGAG AAGTCCGAAG GCCTTCCTGT GGCCCCATTC 
ATGGACCGAG ACAAAGTGAC CAAAGCGACA GCCCAAATTG GGTTCATCAA GTTTGTCCTA 
ATCCCAATGT TTGAAACAGT GACCAAGCTC TTCCCCATTG TCGAGGAGAC CATGCTGCGG 
CCCCTCTGGG AGTCCCGAGA ACACTACGAG GAGCTGAAGC AGCTGGACGA CGCCATGAAG 
GAGCTGCAGA AGAAAACGGA GAACTTAACA TCTGGGGCCA CGGAAAACGC CCCAGAGAAG 
ACCAGAGATG CGAAAGACAA TGAAGACCGT TCACCTCCAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008771017.1
CDS	59..1663
Translation	MGAGSSSYRPKAIYLDIDGRIQKVVFSKYCNSSDIMDLFCIATGLPRNTTISLLTTDDAMVSIDPTMPANSERTPYKVRPVAVKQVSEREELVQGVLAQVAEQFSRAFKINELKAEVANHLAMLEKRVELEGLKVVEIEKCKSDIKKMREELAARNNRTNCPCKYSFLDNKKLTPRRDVPTYPKYLLSPETIEALRKPTFDVWLWEPNEMLSCLEHMYHDLGLVRDFSINPITLRRWLLCVHDNYRSNPFHNFRHCFCVTQMMYSMVWLCGLQEKFSQMDILVLMTAAICHDLDHPGYNNTYQINARTELAVRYNDISPLENHHCAIAFQILARPECNIFASVPPEGFRQIRQGMITLILATDMARHAEIMDSFKEKMENFDYSNEEHLTLLKMILIKCCDISNEVRPMEVAEPWVDCLLEEYFMQSDREKSEGLPVAPFMDRDKVTKATAQIGFIKFVLIPMFETVTKLFPIVEETMLRPLWESREHYEELKQLDDAMKELQKKTENLTSGATENAPEKTRDAKDNEDRSPPN

Target ORF information:

RefSeq Version	XM_008772795.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphodiesterase 9A (Pde9a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772795.2

ORF Insert Sequence:

ATGGGGGCCG GCTCCTCAAG CTACCGGCCC AAGGCCATCT ACCTGGACAT CGATGGACGC 
ATCCAGAAGG TGGTTTTCAG CAAGTATTGT AACTCCAGTG ACATCATGGA CCTGTTCTGT 
ATCGCCACCG GCCTGCCACG GAATACCACC ATCTCCCTTC TGACCACAGA CGACGCCATG 
GTCTCCATCG ATCCCACCAT GCCTGCGAAT TCAGAGCGAA CTCCCTACAA GGTGAGACCT 
GTGGCTGTCA AGCAAGTGTC TGAAAGAGAA GAACTCGTCC AGGGTGTGCT GGCCCAAGTG 
GCGGAACAAT TCTCCAGAGC GTTTAAGATC AACGAGCTGA AAGCTGAAGT TGCGAATCAC 
CTGGCCATGC TGGAGAAACG GGTGGAATTG GAAGGACTCA AAGTGGTGGA GATCGAAAAA 
TGCAAGAGTG ATATTAAAAA GATGCGGGAG GAGCTGGCAG CTAGAAACAA CAGGACCAAC 
TGTCCCTGTA AATACAGTTT TTTGGATAAC AAGAAGTTGA CACCTCGACG TGATGTCCCC 
ACTTACCCCA AGTACCTGCT CTCCCCAGAA ACCATTGAAG CCCTACGGAA GCCCACCTTT 
GATGTCTGGC TTTGGGAACC CAATGAGATG CTGAGCTGTT TAGAGCATAT GTACCATGAC 
CTTGGTCTGG TCAGGGACTT CAGCATCAAC CCAATCACAC TCCGCAGGTG GCTGCTCTGT 
GTGCATGACA ACTACAGGAG CAACCCCTTC CACAACTTCC GGCACTGCTT CTGTGTGACA 
CAGATGATGT ACAGCATGGT CTGGCTCTGT GGCCTCCAGG AGAAGTTTTC CCAGATGGAC 
ATCTTGGTCC TGATGACCGC AGCCATCTGC CATGACCTGG ACCACCCAGG GTACAACAAC 
ACATACCAGA TCAACGCCCG CACGGAACTA GCGGTGCGCT ACAACGACAT CTCGCCGCTG 
GAGAACCACC ATTGCGCCAT CGCCTTCCAG ATCCTGGCGA GACCCGAGTG CAACATCTTC 
GCCAGCGTGC CGCCTGAGGG CTTCAGGCAG ATCAGGCAGG GGATGATCAC GTTGATCTTG 
GCCACCGACA TGGCAAGGCA CGCTGAAATT ATGGATTCTT TCAAAGAAAA AATGGAGAAT 
TTTGACTACA GCAATGAGGA GCATCTGACC CTGCTGAAGA TGATCCTCAT AAAATGCTGT 
GACATCTCCA ATGAGGTCCG TCCCATGGAG GTGGCAGAAC CGTGGGTGGA CTGTTTACTG 
GAAGAATATT TTATGCAGAG TGACCGCGAG AAGTCCGAAG GCCTTCCTGT GGCCCCATTC 
ATGGACCGAG ACAAAGTGAC CAAAGCGACA GCCCAAATTG GGTTCATCAA GTTTGTCCTA 
ATCCCAATGT TTGAAACAGT GACCAAGCTC TTCCCCATTG TCGAGGAGAC CATGCTGCGG 
CCCCTCTGGG AGTCCCGAGA ACACTACGAG GAGCTGAAGC AGCTGGACGA CGCCATGAAG 
GAGCTGCAGA AGAAAACGGA GAACTTAACA TCTGGGGCCA CGGAAAACGC CCCAGAGAAG 
ACCAGAGATG CGAAAGACAA TGAAGACCGT TCACCTCCAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde9a ( XM_008772793.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008771015.1 Rattus norvegicus phosphodiesterase 9A (Pde9a), transcript variant X1, mRNA.

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CloneID	ORa44355
Clone ID Related Accession (Same CDS sequence)	XM_008772794.2 , XM_008772795.2 , XM_008772793.2
Accession Version	XM_008772793.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	high affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772793.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCCG GCTCCTCAAG CTACCGGCCC AAGGCCATCT ACCTGGACAT CGATGGACGC 
ATCCAGAAGG TGGTTTTCAG CAAGTATTGT AACTCCAGTG ACATCATGGA CCTGTTCTGT 
ATCGCCACCG GCCTGCCACG GAATACCACC ATCTCCCTTC TGACCACAGA CGACGCCATG 
GTCTCCATCG ATCCCACCAT GCCTGCGAAT TCAGAGCGAA CTCCCTACAA GGTGAGACCT 
GTGGCTGTCA AGCAAGTGTC TGAAAGAGAA GAACTCGTCC AGGGTGTGCT GGCCCAAGTG 
GCGGAACAAT TCTCCAGAGC GTTTAAGATC AACGAGCTGA AAGCTGAAGT TGCGAATCAC 
CTGGCCATGC TGGAGAAACG GGTGGAATTG GAAGGACTCA AAGTGGTGGA GATCGAAAAA 
TGCAAGAGTG ATATTAAAAA GATGCGGGAG GAGCTGGCAG CTAGAAACAA CAGGACCAAC 
TGTCCCTGTA AATACAGTTT TTTGGATAAC AAGAAGTTGA CACCTCGACG TGATGTCCCC 
ACTTACCCCA AGTACCTGCT CTCCCCAGAA ACCATTGAAG CCCTACGGAA GCCCACCTTT 
GATGTCTGGC TTTGGGAACC CAATGAGATG CTGAGCTGTT TAGAGCATAT GTACCATGAC 
CTTGGTCTGG TCAGGGACTT CAGCATCAAC CCAATCACAC TCCGCAGGTG GCTGCTCTGT 
GTGCATGACA ACTACAGGAG CAACCCCTTC CACAACTTCC GGCACTGCTT CTGTGTGACA 
CAGATGATGT ACAGCATGGT CTGGCTCTGT GGCCTCCAGG AGAAGTTTTC CCAGATGGAC 
ATCTTGGTCC TGATGACCGC AGCCATCTGC CATGACCTGG ACCACCCAGG GTACAACAAC 
ACATACCAGA TCAACGCCCG CACGGAACTA GCGGTGCGCT ACAACGACAT CTCGCCGCTG 
GAGAACCACC ATTGCGCCAT CGCCTTCCAG ATCCTGGCGA GACCCGAGTG CAACATCTTC 
GCCAGCGTGC CGCCTGAGGG CTTCAGGCAG ATCAGGCAGG GGATGATCAC GTTGATCTTG 
GCCACCGACA TGGCAAGGCA CGCTGAAATT ATGGATTCTT TCAAAGAAAA AATGGAGAAT 
TTTGACTACA GCAATGAGGA GCATCTGACC CTGCTGAAGA TGATCCTCAT AAAATGCTGT 
GACATCTCCA ATGAGGTCCG TCCCATGGAG GTGGCAGAAC CGTGGGTGGA CTGTTTACTG 
GAAGAATATT TTATGCAGAG TGACCGCGAG AAGTCCGAAG GCCTTCCTGT GGCCCCATTC 
ATGGACCGAG ACAAAGTGAC CAAAGCGACA GCCCAAATTG GGTTCATCAA GTTTGTCCTA 
ATCCCAATGT TTGAAACAGT GACCAAGCTC TTCCCCATTG TCGAGGAGAC CATGCTGCGG 
CCCCTCTGGG AGTCCCGAGA ACACTACGAG GAGCTGAAGC AGCTGGACGA CGCCATGAAG 
GAGCTGCAGA AGAAAACGGA GAACTTAACA TCTGGGGCCA CGGAAAACGC CCCAGAGAAG 
ACCAGAGATG CGAAAGACAA TGAAGACCGT TCACCTCCAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008771015.1
CDS	59..1663
Translation	MGAGSSSYRPKAIYLDIDGRIQKVVFSKYCNSSDIMDLFCIATGLPRNTTISLLTTDDAMVSIDPTMPANSERTPYKVRPVAVKQVSEREELVQGVLAQVAEQFSRAFKINELKAEVANHLAMLEKRVELEGLKVVEIEKCKSDIKKMREELAARNNRTNCPCKYSFLDNKKLTPRRDVPTYPKYLLSPETIEALRKPTFDVWLWEPNEMLSCLEHMYHDLGLVRDFSINPITLRRWLLCVHDNYRSNPFHNFRHCFCVTQMMYSMVWLCGLQEKFSQMDILVLMTAAICHDLDHPGYNNTYQINARTELAVRYNDISPLENHHCAIAFQILARPECNIFASVPPEGFRQIRQGMITLILATDMARHAEIMDSFKEKMENFDYSNEEHLTLLKMILIKCCDISNEVRPMEVAEPWVDCLLEEYFMQSDREKSEGLPVAPFMDRDKVTKATAQIGFIKFVLIPMFETVTKLFPIVEETMLRPLWESREHYEELKQLDDAMKELQKKTENLTSGATENAPEKTRDAKDNEDRSPPN

Target ORF information:

RefSeq Version	XM_008772793.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphodiesterase 9A (Pde9a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772793.2

ORF Insert Sequence:

ATGGGGGCCG GCTCCTCAAG CTACCGGCCC AAGGCCATCT ACCTGGACAT CGATGGACGC 
ATCCAGAAGG TGGTTTTCAG CAAGTATTGT AACTCCAGTG ACATCATGGA CCTGTTCTGT 
ATCGCCACCG GCCTGCCACG GAATACCACC ATCTCCCTTC TGACCACAGA CGACGCCATG 
GTCTCCATCG ATCCCACCAT GCCTGCGAAT TCAGAGCGAA CTCCCTACAA GGTGAGACCT 
GTGGCTGTCA AGCAAGTGTC TGAAAGAGAA GAACTCGTCC AGGGTGTGCT GGCCCAAGTG 
GCGGAACAAT TCTCCAGAGC GTTTAAGATC AACGAGCTGA AAGCTGAAGT TGCGAATCAC 
CTGGCCATGC TGGAGAAACG GGTGGAATTG GAAGGACTCA AAGTGGTGGA GATCGAAAAA 
TGCAAGAGTG ATATTAAAAA GATGCGGGAG GAGCTGGCAG CTAGAAACAA CAGGACCAAC 
TGTCCCTGTA AATACAGTTT TTTGGATAAC AAGAAGTTGA CACCTCGACG TGATGTCCCC 
ACTTACCCCA AGTACCTGCT CTCCCCAGAA ACCATTGAAG CCCTACGGAA GCCCACCTTT 
GATGTCTGGC TTTGGGAACC CAATGAGATG CTGAGCTGTT TAGAGCATAT GTACCATGAC 
CTTGGTCTGG TCAGGGACTT CAGCATCAAC CCAATCACAC TCCGCAGGTG GCTGCTCTGT 
GTGCATGACA ACTACAGGAG CAACCCCTTC CACAACTTCC GGCACTGCTT CTGTGTGACA 
CAGATGATGT ACAGCATGGT CTGGCTCTGT GGCCTCCAGG AGAAGTTTTC CCAGATGGAC 
ATCTTGGTCC TGATGACCGC AGCCATCTGC CATGACCTGG ACCACCCAGG GTACAACAAC 
ACATACCAGA TCAACGCCCG CACGGAACTA GCGGTGCGCT ACAACGACAT CTCGCCGCTG 
GAGAACCACC ATTGCGCCAT CGCCTTCCAG ATCCTGGCGA GACCCGAGTG CAACATCTTC 
GCCAGCGTGC CGCCTGAGGG CTTCAGGCAG ATCAGGCAGG GGATGATCAC GTTGATCTTG 
GCCACCGACA TGGCAAGGCA CGCTGAAATT ATGGATTCTT TCAAAGAAAA AATGGAGAAT 
TTTGACTACA GCAATGAGGA GCATCTGACC CTGCTGAAGA TGATCCTCAT AAAATGCTGT 
GACATCTCCA ATGAGGTCCG TCCCATGGAG GTGGCAGAAC CGTGGGTGGA CTGTTTACTG 
GAAGAATATT TTATGCAGAG TGACCGCGAG AAGTCCGAAG GCCTTCCTGT GGCCCCATTC 
ATGGACCGAG ACAAAGTGAC CAAAGCGACA GCCCAAATTG GGTTCATCAA GTTTGTCCTA 
ATCCCAATGT TTGAAACAGT GACCAAGCTC TTCCCCATTG TCGAGGAGAC CATGCTGCGG 
CCCCTCTGGG AGTCCCGAGA ACACTACGAG GAGCTGAAGC AGCTGGACGA CGCCATGAAG 
GAGCTGCAGA AGAAAACGGA GAACTTAACA TCTGGGGCCA CGGAAAACGC CCCAGAGAAG 
ACCAGAGATG CGAAAGACAA TGAAGACCGT TCACCTCCAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde9a ( XM_017601537.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017457026.1 Rattus norvegicus phosphodiesterase 9A (Pde9a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa60034
Clone ID Related Accession (Same CDS sequence)	XM_017601537.1
Accession Version	XM_017601537.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	high affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGAGA AACGGGTGGA ATTGGAAGGA CTCAAAGTGG TGGAGATCGA AAAATGCAAG 
AGTGATATTA AAAAGATGCG GGAGGAGCTG GCAGCTAGAA ACAACAGGAC CAACTGTCCC 
TGTAAATACA GTTTTTTGGA TAACAAGAAG TTGACACCTC GACGTGATGT CCCCACTTAC 
CCCAAGTACC TGCTCTCCCC AGAAACCATT GAAGCCCTAC GGAAGCCCAC CTTTGATGTC 
TGGCTTTGGG AACCCAATGA GATGCTGAGC TGTTTAGAGC ATATGTACCA TGACCTTGGT 
CTGGTCAGGG ACTTCAGCAT CAACCCAATC ACACTCCGCA GGTGGCTGCT CTGTGTGCAT 
GACAACTACA GGAGCAACCC CTTCCACAAC TTCCGGCACT GCTTCTGTGT GACACAGATG 
ATGTACAGCA TGGTCTGGCT CTGTGGCCTC CAGGAGAAGT TTTCCCAGAT GGACATCTTG 
GTCCTGATGA CCGCAGCCAT CTGCCATGAC CTGGACCACC CAGGGTACAA CAACACATAC 
CAGATCAACG CCCGCACGGA ACTAGCGGTG CGCTACAACG ACATCTCGCC GCTGGAGAAC 
CACCATTGCG CCATCGCCTT CCAGATCCTG GCGAGACCCG AGTGCAACAT CTTCGCCAGC 
GTGCCGCCTG AGGGCTTCAG GCAGATCAGG CAGGGGATGA TCACGTTGAT CTTGGCCACC 
GACATGGCAA GGCACGCTGA AATTATGGAT TCTTTCAAAG AAAAAATGGA GAATTTTGAC 
TACAGCAATG AGGAGCATCT GACCCTGCTG AAGATGATCC TCATAAAATG CTGTGACATC 
TCCAATGAGG TCCGTCCCAT GGAGGTGGCA GAACCGTGGG TGGACTGTTT ACTGGAAGAA 
TATTTTATGC AGAGTGACCG CGAGAAGTCC GAAGGCCTTC CTGTGGCCCC ATTCATGGAC 
CGAGACAAAG TGACCAAAGC GACAGCCCAA ATTGGGTTCA TCAAGTTTGT CCTAATCCCA 
ATGTTTGAAA CAGTGACCAA GCTCTTCCCC ATTGTCGAGG AGACCATGCT GCGGCCCCTC 
TGGGAGTCCC GAGAACACTA CGAGGAGCTG AAGCAGCTGG ACGACGCCAT GAAGGAGCTG 
CAGAAGAAAA CGGAGAACTT AACATCTGGG GCCACGGAAA ACGCCCCAGA GAAGACCAGA 
GATGCGAAAG ACAATGAAGA CCGTTCACCT CCAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017457026.1
CDS	389..1627
Translation	MLEKRVELEGLKVVEIEKCKSDIKKMREELAARNNRTNCPCKYSFLDNKKLTPRRDVPTYPKYLLSPETIEALRKPTFDVWLWEPNEMLSCLEHMYHDLGLVRDFSINPITLRRWLLCVHDNYRSNPFHNFRHCFCVTQMMYSMVWLCGLQEKFSQMDILVLMTAAICHDLDHPGYNNTYQINARTELAVRYNDISPLENHHCAIAFQILARPECNIFASVPPEGFRQIRQGMITLILATDMARHAEIMDSFKEKMENFDYSNEEHLTLLKMILIKCCDISNEVRPMEVAEPWVDCLLEEYFMQSDREKSEGLPVAPFMDRDKVTKATAQIGFIKFVLIPMFETVTKLFPIVEETMLRPLWESREHYEELKQLDDAMKELQKKTENLTSGATENAPEKTRDAKDNEDRSPPN

Target ORF information:

RefSeq Version	XM_017601537.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphodiesterase 9A (Pde9a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017601537.1

ORF Insert Sequence:

ATGCTGGAGA AACGGGTGGA ATTGGAAGGA CTCAAAGTGG TGGAGATCGA AAAATGCAAG 
AGTGATATTA AAAAGATGCG GGAGGAGCTG GCAGCTAGAA ACAACAGGAC CAACTGTCCC 
TGTAAATACA GTTTTTTGGA TAACAAGAAG TTGACACCTC GACGTGATGT CCCCACTTAC 
CCCAAGTACC TGCTCTCCCC AGAAACCATT GAAGCCCTAC GGAAGCCCAC CTTTGATGTC 
TGGCTTTGGG AACCCAATGA GATGCTGAGC TGTTTAGAGC ATATGTACCA TGACCTTGGT 
CTGGTCAGGG ACTTCAGCAT CAACCCAATC ACACTCCGCA GGTGGCTGCT CTGTGTGCAT 
GACAACTACA GGAGCAACCC CTTCCACAAC TTCCGGCACT GCTTCTGTGT GACACAGATG 
ATGTACAGCA TGGTCTGGCT CTGTGGCCTC CAGGAGAAGT TTTCCCAGAT GGACATCTTG 
GTCCTGATGA CCGCAGCCAT CTGCCATGAC CTGGACCACC CAGGGTACAA CAACACATAC 
CAGATCAACG CCCGCACGGA ACTAGCGGTG CGCTACAACG ACATCTCGCC GCTGGAGAAC 
CACCATTGCG CCATCGCCTT CCAGATCCTG GCGAGACCCG AGTGCAACAT CTTCGCCAGC 
GTGCCGCCTG AGGGCTTCAG GCAGATCAGG CAGGGGATGA TCACGTTGAT CTTGGCCACC 
GACATGGCAA GGCACGCTGA AATTATGGAT TCTTTCAAAG AAAAAATGGA GAATTTTGAC 
TACAGCAATG AGGAGCATCT GACCCTGCTG AAGATGATCC TCATAAAATG CTGTGACATC 
TCCAATGAGG TCCGTCCCAT GGAGGTGGCA GAACCGTGGG TGGACTGTTT ACTGGAAGAA 
TATTTTATGC AGAGTGACCG CGAGAAGTCC GAAGGCCTTC CTGTGGCCCC ATTCATGGAC 
CGAGACAAAG TGACCAAAGC GACAGCCCAA ATTGGGTTCA TCAAGTTTGT CCTAATCCCA 
ATGTTTGAAA CAGTGACCAA GCTCTTCCCC ATTGTCGAGG AGACCATGCT GCGGCCCCTC 
TGGGAGTCCC GAGAACACTA CGAGGAGCTG AAGCAGCTGG ACGACGCCAT GAAGGAGCTG 
CAGAAGAAAA CGGAGAACTT AACATCTGGG GCCACGGAAA ACGCCCCAGA GAAGACCAGA 
GATGCGAAAG ACAATGAAGA CCGTTCACCT CCAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Cell164(4)805-17(2016 Feb)
Yang X,Coulombe-Huntington J,Kang S,Sheynkman GM,Hao T,Richardson A,Sun S,Yang F,Shen YA,Murray RR,Spirohn K,Begg BE,Duran-Frigola M,MacWilliams A,Pevzner SJ,Zhong Q,Trigg SA,Tam S,Ghamsari L,Sahni N,Yi S,Rodriguez MD,Balcha D,Tan G,Costanzo M,Andrews B,Boone C,Zhou XJ,Salehi-Ashtiani K,Charloteaux B,Chen AA,Calderwood MA,Aloy P,Roth FP,Hill DE,Iakoucheva LM,Xia Y,Vidal M

Phosphodiesterase 9A controls nitric-oxide-independent cGMP and hypertrophic heart disease.
Nature519(7544)472-6(2015 Mar)
Lee DI,Zhu G,Sasaki T,Cho GS,Hamdani N,Holewinski R,Jo SH,Danner T,Zhang M,Rainer PP,Bedja D,Kirk JA,Ranek MJ,Dostmann WR,Kwon C,Margulies KB,Van Eyk JE,Paulus WJ,Takimoto E,Kass DA

PDE9A inhibition rescues amyloid beta-induced deficits in synaptic plasticity and cognition.
Neurobiology of aging35(9)2072-8(2014 Sep)
Kroker KS,Mathis C,Marti A,Cassel JC,Rosenbrock H,Dorner-Ciossek C

PDE9A, PDE10A, and PDE11A expression in rat trigeminovascular pain signalling system.
Brain research128125-34(2009 Jul)
Kruse LS,M?ller M,Tibaek M,Gammeltoft S,Olesen J,Kruuse C

Cell array-based intracellular localization screening reveals novel functional features of human chromosome 21 proteins.
BMC genomics7155(2006 Jun)
Hu YH,Warnatz HJ,Vanhecke D,Wagner F,Fiebitz A,Thamm S,Kahlem P,Lehrach H,Yaspo ML,Janitz M

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Pde9a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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