Defb22 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Defb22
Entrez Gene ID	171412
Full Name	defensin beta 22
Synonyms	2D6glycoprotein
General protein information	Preferred Names defensin beta 22 Names beta-defensin 126 2D6 glycoprotein E-3 epididymal fluid protein beta-defensin 22
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q41
Summary	may play a role in antimicrobial defense of sperm in the epididymis and in the female reproductive tract [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_134391.1	NP_599218.1	beta-defensin 126 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Defb22	NP_001002791.1
Rattus norvegicus (Norway rat)	Defb22	NP_599218.1

Gene Ontology
Bibliography

Related articles in PubMed

Rat recombinant ?-defensin 22 is a heparin-binding protein with antimicrobial activity.
Diao H, Yu HG, Sun F, Zhang YL, Tanphaichitr N
Asian journal of andrology13(2)305-11(2011 Mar)

Soluble fusion expression and characterization of bioactive human beta-defensin 26 and 27.
Huang L, Leong SS, Jiang R
Applied microbiology and biotechnology84(2)301-8(2009 Aug)

Beta-defensin 22 is a major component of the mouse sperm glycocalyx.
Yudin AI, Tollner TL, Treece CA, Kays R, Cherr GN, Overstreet JW, Bevins CL
Reproduction (Cambridge, England)136(6)753-65(2008 Dec)

Cross-species analysis of the mammalian beta-defensin gene family: presence of syntenic gene clusters and preferential expression in the male reproductive tract.
Patil AA, Cai Y, Sang Y, Blecha F, Zhang G
Physiological genomics23(1)5-17(2005 Sep)

Cloning and characterization of a novel sperm-associated isoantigen (E-3) with defensin- and lectin-like motifs expressed in rat epididymis.
Rao J, Herr JC, Reddi PP, Wolkowicz MJ, Bush LA, Sherman NE, Black M, Flickinger CJ
Biology of reproduction68(1)290-301(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Demonstrated the antimicrobial activity of recombinant beta-defensin 22 against E coli and Candida albicans. It has lectin-like ability, binding to heparin beads. This heparin-binding activity implies potential roles in fertilisation capabilities of sperm.
Title: Rat recombinant ?-defensin 22 is a heparin-binding protein with antimicrobial activity.

The following Defb22 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Defb22 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42022	NM_134391.1 Latest version!	Rattus norvegicus defensin beta 22 (Defb22), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Defb22 ( NM_134391.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_599218.1 Rattus norvegicus defensin beta 22 (Defb22), mRNA.

Price & Availability↑

CloneID	ORa42022
Clone ID Related Accession (Same CDS sequence)	NM_134391.1
Accession Version	NM_134391.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Rattus norvegicus(Norway rat)
Product	beta-defensin 126 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ309150.1. ##Evidence-Data-START## Transcript exon combination :: AJ309150.1, AY621353.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02642711 [ECO:0000348] ##Evidence-Data-END##

ATGAAGTCCC TATTGTCTGC TCTTATGATC ATTATGTTCC TGGCCCACTT GGTCACAGGT 
AACTGGTACG TTCGAAAGTG TGCAAACAAA TTGGGCACCT GTAGGAAGAC GTGCAGAAAA 
GGGGAGTACC AGACAGACCC TGCAACGGGG AAATGCTCCA TAGGAAAACT GTGCTGTATT 
CTGGACCTCA AACTTGCTGG TCAATGTGGG GGCGCAGATG GAAACCAAGC TGCTGCGGGG 
ACACAGGCGG CAGGGGGGAC ACGGGCGGCA GGGGGAACAC AGGGAACAGG GGGAACTGGG 
GCCACAGGTG CAGCGGCCAC CACTGCTGCA CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_599218.1
CDS	259..594
Translation	MKSLLSALMIIMFLAHLVTGNWYVRKCANKLGTCRKTCRKGEYQTDPATGKCSIGKLCCILDLKLAGQCGGADGNQAAAGTQAAGGTRAAGGTQGTGGTGATGAAATTAAP

Target ORF information:

RefSeq Version	NM_134391.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus defensin beta 22 (Defb22), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134391.1

ORF Insert Sequence:

ATGAAGTCCC TATTGTCTGC TCTTATGATC ATTATGTTCC TGGCCCACTT GGTCACAGGT 
AACTGGTACG TTCGAAAGTG TGCAAACAAA TTGGGCACCT GTAGGAAGAC GTGCAGAAAA 
GGGGAGTACC AGACAGACCC TGCAACGGGG AAATGCTCCA TAGGAAAACT GTGCTGTATT 
CTGGACCTCA AACTTGCTGG TCAATGTGGG GGCGCAGATG GAAACCAAGC TGCTGCGGGG 
ACACAGGCGG CAGGGGGGAC ACGGGCGGCA GGGGGAACAC AGGGAACAGG GGGAACTGGG 
GCCACAGGTG CAGCGGCCAC CACTGCTGCA CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Rat recombinant ?-defensin 22 is a heparin-binding protein with antimicrobial activity.
Asian journal of andrology13(2)305-11(2011 Mar)
Diao H,Yu HG,Sun F,Zhang YL,Tanphaichitr N

Soluble fusion expression and characterization of bioactive human beta-defensin 26 and 27.
Applied microbiology and biotechnology84(2)301-8(2009 Aug)
Huang L,Leong SS,Jiang R

Beta-defensin 22 is a major component of the mouse sperm glycocalyx.
Reproduction (Cambridge, England)136(6)753-65(2008 Dec)
Yudin AI,Tollner TL,Treece CA,Kays R,Cherr GN,Overstreet JW,Bevins CL

Cross-species analysis of the mammalian beta-defensin gene family: presence of syntenic gene clusters and preferential expression in the male reproductive tract.
Physiological genomics23(1)5-17(2005 Sep)
Patil AA,Cai Y,Sang Y,Blecha F,Zhang G

Cloning and characterization of a novel sperm-associated isoantigen (E-3) with defensin- and lectin-like motifs expressed in rat epididymis.
Biology of reproduction68(1)290-301(2003 Jan)
Rao J,Herr JC,Reddi PP,Wolkowicz MJ,Bush LA,Sherman NE,Black M,Flickinger CJ