Elovl5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Elovl5
Entrez Gene ID	171400
Full Name	ELOVL fatty acid elongase 5
Synonyms	rELO1
General protein information	Preferred Names ELOVL fatty acid elongase 5 Names elongation of very long chain fatty acids protein 5 3-keto acyl-CoA synthase Elovl5 ELOVL FA elongase 5 ELOVL family member 5, elongation of long chain fatty acids elongation of long chain fatty acids family member 5 elongation of very long chain fatty acids-like 5 fatty acid elongase 1 very long chain 3-ketoacyl-CoA synthase 5 very long chain 3-oxoacyl-CoA synthase 5
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q31
Summary	human homolog is involved in the elongation of long-chain polyunsaturated fatty acids [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006243417.2	XP_006243479.1	elongation of very long chain fatty acids protein 5 isoform X1
NM_134382.1	NP_599209.1	elongation of very long chain fatty acids protein 5

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Elovl5	NP_599209.1
Xenopus tropicalis (tropical clawed frog)	elovl5	NP_001011248.1
Saccharomyces cerevisiae (baker's yeast)	FEN1	NP_009963.1
Neurospora crassa	NCU06694	XP_961009.1
Homo sapiens (human)	ELOVL5	NP_068586.1
Mus musculus (house mouse)	Elovl5	NP_599016.2
Eremothecium gossypii	AGOS_AFR624W	NP_986171.1
Magnaporthe oryzae (rice blast fungus)	MGG_07280	XP_003715505.1
Arabidopsis thaliana (thale cress)	AT3G06470	NP_187298.1
Canis lupus familiaris (dog)	ELOVL5	XP_005627538.1
Arabidopsis thaliana (thale cress)	AT3G06460	NP_187297.1
Pan troglodytes (chimpanzee)	ELOVL5	NP_001233554.1
Bos taurus (cattle)	ELOVL5	NP_001040062.1
Gallus gallus (chicken)	ELOVL5	NP_001186126.1
Danio rerio (zebrafish)	elovl5	NP_956747.1
Kluyveromyces lactis	KLLA0C03542g	XP_452354.1
Schizosaccharomyces pombe (fission yeast)	SPAC1B2.03c	NP_593930.1
Oryza sativa (rice)	Os03g0701500	NP_001051002.1

Gene Ontology
Bibliography

Related articles in PubMed

Role of maternal tissue in the synthesis of polyunsaturated fatty acids in response to a lipid-deficient diet during pregnancy and lactation in rats.
Gonz?lez RS, Rodriguez-Cruz M, Maldonado J, Saavedra FJ
Gene549(1)7-23(2014 Oct)

ELOVL5 mutations cause spinocerebellar ataxia 38.
Di Gregorio E, Borroni B, Giorgio E, Lacerenza D, Ferrero M, Lo Buono N, Ragusa N, Mancini C, Gaussen M, Calcia A, Mitro N, Hoxha E, Mura I, Coviello DA, Moon YA, Tesson C, Vaula G, Couarch P, Orsi L, Duregon E, Papotti MG, Deleuze JF, Imbert J, Costanzi C, Padovani A, Giunti P, Maillet-Vioud M, Durr A, Brice A, Tempia F, Funaro A, Boccone L, Caruso D, Stevanin G, Brusco A
American journal of human genetics95(2)209-17(2014 Aug)

Molecular basis for differential elongation of omega-3 docosapentaenoic acid by the rat Elovl5 and Elovl2.
Gregory MK, Cleland LG, James MJ
Journal of lipid research54(10)2851-7(2013 Oct)

MicroRNA-30c reduces hyperlipidemia and atherosclerosis in mice by decreasing lipid synthesis and lipoprotein secretion.
Soh J, Iqbal J, Queiroz J, Fernandez-Hernando C, Hussain MM
Nature medicine19(7)892-900(2013 Jul)

Elongase reactions as control points in long-chain polyunsaturated fatty acid synthesis.
Gregory MK, Gibson RA, Cook-Johnson RJ, Cleland LG, James MJ
PloS one6(12)e29662(2011)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results show that genes with the highest expression in the mammary gland, liver and adipose tissue were Elovl5, Fads2, and Fasn, respectively, in a low-lipid diet than in adequate-lipid diet.
Title: Role of maternal tissue in the synthesis of polyunsaturated fatty acids in response to a lipid-deficient diet during pregnancy and lactation in rats.

Critical residues in Elovl2 and Elovl5 define their substrate specificities and their ability to elongate docosapentaenoic acid.
Title: Molecular basis for differential elongation of omega-3 docosapentaenoic acid by the rat Elovl5 and Elovl2.

These findings demonstrate that coordinated induction of fatty acid elongases Elovl5 and 6 and desaturase activity is required for balanced synthesis of specific n-7 versus n-9 MUFA species.
Title: Role of fatty acid elongases in determination of de novo synthesized monounsaturated fatty acid species.

The following Elovl5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Elovl5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12603	NM_134382.1 Latest version!	Rattus norvegicus ELOVL fatty acid elongase 5 (Elovl5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa12603	XM_006243417.2 Latest version!	Rattus norvegicus ELOVL fatty acid elongase 5 (Elovl5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Elovl5 ( NM_134382.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_599209.1 Rattus norvegicus ELOVL fatty acid elongase 5 (Elovl5), mRNA.

Price & Availability↑

CloneID	ORa12603
Clone ID Related Accession (Same CDS sequence)	XM_006243417.2 , NM_134382.1
Accession Version	NM_134382.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Rattus norvegicus(Norway rat)
Product	elongation of very long chain fatty acids protein 5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB071985.1. On Jun 25, 2006 this sequence version replaced XM_001075440.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB071985.1, HQ404314.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGAACATT TCGATGCGTC ACTCAGTACC TATTTCAGGG CATTACTGGG CCCCCGAGAC 
ACACGAGTCA AAGGATGGTT TCTTCTGGAC AATTACATCC CTACGTTTGT CTGCTCTGCC 
ATTTATTTAC TCATCGTGTG GCTGGGACCA AAATACATGA AAAACAGGCA GCCGTTCTCT 
TGCCGAGGCA TCCTGGTGGT GTATAATCTT GGGCTCACCC TGCTGTCTCT CTACATGTTC 
TATGAGTTGG TGACAGGTGT GTGGGAAGGC AAATACAACT TCTTCTGTCA GGGAACACGC 
AGCGCAGGAG AATCAGATAT GAAGGTTATT CGTGTCCTCT GGTGGTACTA CTTTTCCAAA 
CTCATAGAAT TCATGGACAC CTTTTTCTTC ATTCTTCGTA AGAACAACCA CCAGATCACA 
GTCCTGCACG TCTACCACCA TGCCACTATG CTCAACATCT GGTGGTTTGT CATGAACTGG 
GTTCCCTGCG GCCATTCGTA CTTCGGTGCG ACGCTCAACA GCTTCATCCA CGTCCTCATG 
TACTCGTACT ATGGCCTGTC CTCTGTCCCT TCCATGCGTC CCTACCTCTG GTGGAAAAAG 
TACATCACTC AGGGGCAGCT GGTCCAGTTT GTGCTGACAA TCATCCAGAC CAGCTGCGGG 
GTCATCTGGC CGTGCTCCTT CCCTCTCGGG TGGCTGTACT TCCAGATCGG ATACATGATT 
TCCCTGATTG CTCTCTTCAC AAACTTCTAC ATTCAGACTT ACAACAAGAA AGGGGCCTCT 
CGGAGGAAAG AGCACCTGAA GGGCCACCAG AACGGGTCTA TGACTGCCGT CAATGGACAC 
ACCAACAACT TTGCTTCCCT GGAGAACAGT GTGACGTCAA GGAAGCAGCG GAAGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_599209.1
CDS	1..900
Translation	MEHFDASLSTYFRALLGPRDTRVKGWFLLDNYIPTFVCSAIYLLIVWLGPKYMKNRQPFSCRGILVVYNLGLTLLSLYMFYELVTGVWEGKYNFFCQGTRSAGESDMKVIRVLWWYYFSKLIEFMDTFFFILRKNNHQITVLHVYHHATMLNIWWFVMNWVPCGHSYFGATLNSFIHVLMYSYYGLSSVPSMRPYLWWKKYITQGQLVQFVLTIIQTSCGVIWPCSFPLGWLYFQIGYMISLIALFTNFYIQTYNKKGASRRKEHLKGHQNGSMTAVNGHTNNFASLENSVTSRKQRKD

Target ORF information:

RefSeq Version	NM_134382.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ELOVL fatty acid elongase 5 (Elovl5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134382.1

ORF Insert Sequence:

ATGGAACATT TCGATGCGTC ACTCAGTACC TATTTCAGGG CATTACTGGG CCCCCGAGAC 
ACACGAGTCA AAGGATGGTT TCTTCTGGAC AATTACATCC CTACGTTTGT CTGCTCTGCC 
ATTTATTTAC TCATCGTGTG GCTGGGACCA AAATACATGA AAAACAGGCA GCCGTTCTCT 
TGCCGAGGCA TCCTGGTGGT GTATAATCTT GGGCTCACCC TGCTGTCTCT CTACATGTTC 
TATGAGTTGG TGACAGGTGT GTGGGAAGGC AAATACAACT TCTTCTGTCA GGGAACACGC 
AGCGCAGGAG AATCAGATAT GAAGGTTATT CGTGTCCTCT GGTGGTACTA CTTTTCCAAA 
CTCATAGAAT TCATGGACAC CTTTTTCTTC ATTCTTCGTA AGAACAACCA CCAGATCACA 
GTCCTGCACG TCTACCACCA TGCCACTATG CTCAACATCT GGTGGTTTGT CATGAACTGG 
GTTCCCTGCG GCCATTCGTA CTTCGGTGCG ACGCTCAACA GCTTCATCCA CGTCCTCATG 
TACTCGTACT ATGGCCTGTC CTCTGTCCCT TCCATGCGTC CCTACCTCTG GTGGAAAAAG 
TACATCACTC AGGGGCAGCT GGTCCAGTTT GTGCTGACAA TCATCCAGAC CAGCTGCGGG 
GTCATCTGGC CGTGCTCCTT CCCTCTCGGG TGGCTGTACT TCCAGATCGG ATACATGATT 
TCCCTGATTG CTCTCTTCAC AAACTTCTAC ATTCAGACTT ACAACAAGAA AGGGGCCTCT 
CGGAGGAAAG AGCACCTGAA GGGCCACCAG AACGGGTCTA TGACTGCCGT CAATGGACAC 
ACCAACAACT TTGCTTCCCT GGAGAACAGT GTGACGTCAA GGAAGCAGCG GAAGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Elovl5 ( XM_006243417.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006243479.1 Rattus norvegicus ELOVL fatty acid elongase 5 (Elovl5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa12603
Clone ID Related Accession (Same CDS sequence)	XM_006243417.2 , NM_134382.1
Accession Version	XM_006243417.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	elongation of very long chain fatty acids protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006243417.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACATT TCGATGCGTC ACTCAGTACC TATTTCAGGG CATTACTGGG CCCCCGAGAC 
ACACGAGTCA AAGGATGGTT TCTTCTGGAC AATTACATCC CTACGTTTGT CTGCTCTGCC 
ATTTATTTAC TCATCGTGTG GCTGGGACCA AAATACATGA AAAACAGGCA GCCGTTCTCT 
TGCCGAGGCA TCCTGGTGGT GTATAATCTT GGGCTCACCC TGCTGTCTCT CTACATGTTC 
TATGAGTTGG TGACAGGTGT GTGGGAAGGC AAATACAACT TCTTCTGTCA GGGAACACGC 
AGCGCAGGAG AATCAGATAT GAAGGTTATT CGTGTCCTCT GGTGGTACTA CTTTTCCAAA 
CTCATAGAAT TCATGGACAC CTTTTTCTTC ATTCTTCGTA AGAACAACCA CCAGATCACA 
GTCCTGCACG TCTACCACCA TGCCACTATG CTCAACATCT GGTGGTTTGT CATGAACTGG 
GTTCCCTGCG GCCATTCGTA CTTCGGTGCG ACGCTCAACA GCTTCATCCA CGTCCTCATG 
TACTCGTACT ATGGCCTGTC CTCTGTCCCT TCCATGCGTC CCTACCTCTG GTGGAAAAAG 
TACATCACTC AGGGGCAGCT GGTCCAGTTT GTGCTGACAA TCATCCAGAC CAGCTGCGGG 
GTCATCTGGC CGTGCTCCTT CCCTCTCGGG TGGCTGTACT TCCAGATCGG ATACATGATT 
TCCCTGATTG CTCTCTTCAC AAACTTCTAC ATTCAGACTT ACAACAAGAA AGGGGCCTCT 
CGGAGGAAAG AGCACCTGAA GGGCCACCAG AACGGGTCTA TGACTGCCGT CAATGGACAC 
ACCAACAACT TTGCTTCCCT GGAGAACAGT GTGACGTCAA GGAAGCAGCG GAAGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006243479.1
CDS	172..1071
Translation	MEHFDASLSTYFRALLGPRDTRVKGWFLLDNYIPTFVCSAIYLLIVWLGPKYMKNRQPFSCRGILVVYNLGLTLLSLYMFYELVTGVWEGKYNFFCQGTRSAGESDMKVIRVLWWYYFSKLIEFMDTFFFILRKNNHQITVLHVYHHATMLNIWWFVMNWVPCGHSYFGATLNSFIHVLMYSYYGLSSVPSMRPYLWWKKYITQGQLVQFVLTIIQTSCGVIWPCSFPLGWLYFQIGYMISLIALFTNFYIQTYNKKGASRRKEHLKGHQNGSMTAVNGHTNNFASLENSVTSRKQRKD

Target ORF information:

RefSeq Version	XM_006243417.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ELOVL fatty acid elongase 5 (Elovl5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006243417.2

ORF Insert Sequence:

ATGGAACATT TCGATGCGTC ACTCAGTACC TATTTCAGGG CATTACTGGG CCCCCGAGAC 
ACACGAGTCA AAGGATGGTT TCTTCTGGAC AATTACATCC CTACGTTTGT CTGCTCTGCC 
ATTTATTTAC TCATCGTGTG GCTGGGACCA AAATACATGA AAAACAGGCA GCCGTTCTCT 
TGCCGAGGCA TCCTGGTGGT GTATAATCTT GGGCTCACCC TGCTGTCTCT CTACATGTTC 
TATGAGTTGG TGACAGGTGT GTGGGAAGGC AAATACAACT TCTTCTGTCA GGGAACACGC 
AGCGCAGGAG AATCAGATAT GAAGGTTATT CGTGTCCTCT GGTGGTACTA CTTTTCCAAA 
CTCATAGAAT TCATGGACAC CTTTTTCTTC ATTCTTCGTA AGAACAACCA CCAGATCACA 
GTCCTGCACG TCTACCACCA TGCCACTATG CTCAACATCT GGTGGTTTGT CATGAACTGG 
GTTCCCTGCG GCCATTCGTA CTTCGGTGCG ACGCTCAACA GCTTCATCCA CGTCCTCATG 
TACTCGTACT ATGGCCTGTC CTCTGTCCCT TCCATGCGTC CCTACCTCTG GTGGAAAAAG 
TACATCACTC AGGGGCAGCT GGTCCAGTTT GTGCTGACAA TCATCCAGAC CAGCTGCGGG 
GTCATCTGGC CGTGCTCCTT CCCTCTCGGG TGGCTGTACT TCCAGATCGG ATACATGATT 
TCCCTGATTG CTCTCTTCAC AAACTTCTAC ATTCAGACTT ACAACAAGAA AGGGGCCTCT 
CGGAGGAAAG AGCACCTGAA GGGCCACCAG AACGGGTCTA TGACTGCCGT CAATGGACAC 
ACCAACAACT TTGCTTCCCT GGAGAACAGT GTGACGTCAA GGAAGCAGCG GAAGGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of maternal tissue in the synthesis of polyunsaturated fatty acids in response to a lipid-deficient diet during pregnancy and lactation in rats.
Gene549(1)7-23(2014 Oct)
Gonz?lez RS,Rodriguez-Cruz M,Maldonado J,Saavedra FJ

ELOVL5 mutations cause spinocerebellar ataxia 38.
American journal of human genetics95(2)209-17(2014 Aug)
Di Gregorio E,Borroni B,Giorgio E,Lacerenza D,Ferrero M,Lo Buono N,Ragusa N,Mancini C,Gaussen M,Calcia A,Mitro N,Hoxha E,Mura I,Coviello DA,Moon YA,Tesson C,Vaula G,Couarch P,Orsi L,Duregon E,Papotti MG,Deleuze JF,Imbert J,Costanzi C,Padovani A,Giunti P,Maillet-Vioud M,Durr A,Brice A,Tempia F,Funaro A,Boccone L,Caruso D,Stevanin G,Brusco A

Molecular basis for differential elongation of omega-3 docosapentaenoic acid by the rat Elovl5 and Elovl2.
Journal of lipid research54(10)2851-7(2013 Oct)
Gregory MK,Cleland LG,James MJ

MicroRNA-30c reduces hyperlipidemia and atherosclerosis in mice by decreasing lipid synthesis and lipoprotein secretion.
Nature medicine19(7)892-900(2013 Jul)
Soh J,Iqbal J,Queiroz J,Fernandez-Hernando C,Hussain MM

Elongase reactions as control points in long-chain polyunsaturated fatty acid synthesis.
PloS one6(12)e29662(2011)
Gregory MK,Gibson RA,Cook-Johnson RJ,Cleland LG,James MJ