Sphk1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sphk1
Entrez Gene ID	170897
Full Name	sphingosine kinase 1
General protein information	Preferred Names sphingosine kinase 1 Names sphingosine kinase 1 SK 1 SPK 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q32.1-q32.2
Summary	catalyzes the conversion of sphingosine to sphingosine 1 phosphate, which plays a role in cell proliferation and differentiation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001270808.1	NP_001257737.1	sphingosine kinase 1 isoform b
NM_133386.3	NP_596877.2	sphingosine kinase 1 isoform b
XM_006247787.2	XP_006247849.1	sphingosine kinase 1 isoform X1
NM_001270810.1	NP_001257739.1	sphingosine kinase 1 isoform b
NM_001270809.1	NP_001257738.1	sphingosine kinase 1 isoform b
NM_001270807.1	NP_001257736.1	sphingosine kinase 1 isoform b
NM_001270811.1	NP_001257740.1	sphingosine kinase 1 isoform a

Pathways from BioSystems

Homologies

	LOC710441	XP_001099066.2
Bos taurus (cattle)	SPHK1	XP_002696250.3
Xenopus tropicalis (tropical clawed frog)	sphk1	NP_001016918.1
Gallus gallus (chicken)	SPHK1	XP_004946176.1
Pan troglodytes (chimpanzee)	SPHK1	XP_003953242.1
Canis lupus familiaris (dog)	SPHK1	XP_540448.3
Homo sapiens (human)	SPHK1	NP_892010.2
Mus musculus (house mouse)	Sphk1	NP_079643.2
Rattus norvegicus (Norway rat)	Sphk1	NP_001257740.1
Danio rerio (zebrafish)	LOC100331840	XP_002667585.2
Drosophila melanogaster (fruit fly)	Sk2	NP_647762.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006995	XP_308775.3

Gene Ontology
Bibliography

Related articles in PubMed

Extracellular vesicles from human-induced pluripotent stem cell-derived mesenchymal stromal cells (hiPSC-MSCs) protect against renal ischemia/reperfusion injury via delivering specificity protein (SP1) and transcriptional activating of sphingosine kinase 1 and inhibiting necroptosis.
Yuan X, Li D, Chen X, Han C, Xu L, Huang T, Dong Z, Zhang M
Cell death & disease8(12)3200(2017 Dec)

Sphk1 mediates neuroinflammation and neuronal injury via TRAF2/NF-?B pathways in activated microglia in cerebral ischemia reperfusion.
Su D, Cheng Y, Li S, Dai D, Zhang W, Lv M
Journal of neuroimmunology30535-41(2017 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In the present study, This study demonstrated that inhibition of Sphk1 decreased IL17 production in microglia after cerebral IR in vivo and in cells cultured under oxygen-glucose deprivation reperfusion conditions.
Title: Sphk1 mediates neuroinflammation and neuronal injury via TRAF2/NF-?B pathways in activated microglia in cerebral ischemia reperfusion.

SPHK1 mediated autophagy is different in neurons and neuroblastoma cells
Title: SPHK1/sphingosine kinase 1-mediated autophagy differs between neurons and SH-SY5Y neuroblastoma cells.

These results indicated that Sphk1/S1P regulates the expression of IL-17A in activated microglia, inducing neuronal apoptosis in cerebral ischemia/reperfusion.
Title: Sphingosine kinase 1/sphingosine-1-phosphate regulates the expression of interleukin-17A in activated microglia in cerebral ischemia/reperfusion.

Inhibition of S1PR2 down-regulate the expressions of SphK1 and MCP-1 in glomerular mesangial cells under high glucose.
Title: [Inhibition of S1PR2 activity down-regulates expressions of sphingosine kinase 1 and MCP-1 in rat glomerular mesangial cells under high glucose].

data indicated that p53 protein and inhibition of SphKs may be early key events responsible for cell death evoked by Abeta1-42
Title: The Molecular Mechanism of Amyloid ?42 Peptide Toxicity: The Role of Sphingosine Kinase-1 and Mitochondrial Sirtuins.

The following Sphk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sphk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa00646	NM_001270809.1 Latest version!	Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa00646	NM_001270810.1 Latest version!	Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa00646	NM_133386.3 Latest version!	Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa00646	NM_001270807.1 Latest version!	Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa00646	NM_001270808.1 Latest version!	Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa09850	NM_001270811.1 Latest version!	Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa33963	XM_006247787.2 Latest version!	Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sphk1 ( NM_001270808.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001257737.1 Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	ORa00646
Clone ID Related Accession (Same CDS sequence)	NM_001270808.1 , NM_133386.3 , NM_001270810.1 , NM_001270809.1 , NM_001270807.1
Accession Version	NM_001270808.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Rattus norvegicus(Norway rat)
Product	sphingosine kinase 1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07030835.1 and BC081738.1. Transcript Variant: This variant (3) uses an alternate first exon compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, 4, 5, and 6 all encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB049571.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAACCAG CAGACTGTCC CCGGGGACTG CTCCCACGGC CATGCAGAGT GTTGGTGCTG 
CTGAACCCCC GGGGTGGCAA GGGCAAGGCT CTGAAGCTCT TTCAGAGCCG TGTGCGGCCC 
CTTCTGGAGG AGGCTGAGGT ATCCTTTAAA CTGATGCTCA CCGAACGGCA GAACCATGCC 
AGGGAGCTGG TGTGTGCAGA AGAACTGGGT CACTGGGATG CCTTGGCGGT CATGTCCGGT 
GACGGTCTGA TGCACGAGGT GGTGAATGGG CTAATGGAAC GACCTGACTG GGAGAGTGCC 
ATCCAGAAAC CCCTATGTAG CCTCCCTGGA GGCTCTGGCA ATGCGCTGGC AGCTTCTTTG 
AACTACTATG CTGGGCACGA GCAGGTCACT AATGAAGATC TCCTGATCAA CTGCACACTG 
CTGTTATGCT GCCGGCAGCT GTCACCCATG AATCTGCTGT CCCTGCACAC CGCTTCCGGA 
CGGCAACTCT ATTCTGTTCT CAGTCTGTCC TGGGGTTTCG TTGCTGACGT GGACTTGGAG 
AGTGAGAAAT ACAGGAGCTT GGGGGAGATT CGTTTCACGG TGGGAACCTT TTTTCGCCTA 
GCAAGCCTGC GCATTTACCA AGGCCAACTG GCCTACCTTC CTGTAGGGAA GGCTGCCTCT 
AAGATACCTG CTTCCTCACT GGCACAGAAG GGCCCTGCCA ACACATACCT CGTTCCTCTG 
GAGGAGCCAG TGCCACCTCA TTGGACTGTG GTGCCAGAGC AGGACTTCGT TCTGGTGCTG 
GTGCTGCTTC ACACCCACCT GAGCACAGAG ATGTTTGCAG CACCCATGGG CCGCTGCGAG 
GCTGGTGTTA TGCATCTGTT CTATATACGT GCGGGGGTGT CAAGGGCCAT GCTGCTGCGC 
CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGACCTCG ACTGCCCGTA CTTGGTTCAT 
GTGCCAGTGG TTGCCTTCCG CCTGGAACCC AGGAACCAGA GGGGAGTGTT TTCTGTGGAC 
GGGGAGCTGA TGGTGTGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG 
GTCTCTGGTA GCAGTGACTC CCCGTCCGGC CGGGACTCCC AGCGGAGGCC ACCTCCAGAA 
GAACCAATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257737.1
CDS	537..1688
Translation	MQPADCPRGLLPRPCRVLVLLNPRGGKGKALKLFQSRVRPLLEEAEVSFKLMLTERQNHARELVCAEELGHWDALAVMSGDGLMHEVVNGLMERPDWESAIQKPLCSLPGGSGNALAASLNYYAGHEQVTNEDLLINCTLLLCCRQLSPMNLLSLHTASGRQLYSVLSLSWGFVADVDLESEKYRSLGEIRFTVGTFFRLASLRIYQGQLAYLPVGKAASKIPASSLAQKGPANTYLVPLEEPVPPHWTVVPEQDFVLVLVLLHTHLSTEMFAAPMGRCEAGVMHLFYIRAGVSRAMLLRLFLAMQKGKHMDLDCPYLVHVPVVAFRLEPRNQRGVFSVDGELMVCEAVQGQVHPNYLWMVSGSSDSPSGRDSQRRPPPEEPI

Target ORF information:

RefSeq Version	NM_001270808.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270808.1

ORF Insert Sequence:

ATGCAACCAG CAGACTGTCC CCGGGGACTG CTCCCACGGC CATGCAGAGT GTTGGTGCTG 
CTGAACCCCC GGGGTGGCAA GGGCAAGGCT CTGAAGCTCT TTCAGAGCCG TGTGCGGCCC 
CTTCTGGAGG AGGCTGAGGT ATCCTTTAAA CTGATGCTCA CCGAACGGCA GAACCATGCC 
AGGGAGCTGG TGTGTGCAGA AGAACTGGGT CACTGGGATG CCTTGGCGGT CATGTCCGGT 
GACGGTCTGA TGCACGAGGT GGTGAATGGG CTAATGGAAC GACCTGACTG GGAGAGTGCC 
ATCCAGAAAC CCCTATGTAG CCTCCCTGGA GGCTCTGGCA ATGCGCTGGC AGCTTCTTTG 
AACTACTATG CTGGGCACGA GCAGGTCACT AATGAAGATC TCCTGATCAA CTGCACACTG 
CTGTTATGCT GCCGGCAGCT GTCACCCATG AATCTGCTGT CCCTGCACAC CGCTTCCGGA 
CGGCAACTCT ATTCTGTTCT CAGTCTGTCC TGGGGTTTCG TTGCTGACGT GGACTTGGAG 
AGTGAGAAAT ACAGGAGCTT GGGGGAGATT CGTTTCACGG TGGGAACCTT TTTTCGCCTA 
GCAAGCCTGC GCATTTACCA AGGCCAACTG GCCTACCTTC CTGTAGGGAA GGCTGCCTCT 
AAGATACCTG CTTCCTCACT GGCACAGAAG GGCCCTGCCA ACACATACCT CGTTCCTCTG 
GAGGAGCCAG TGCCACCTCA TTGGACTGTG GTGCCAGAGC AGGACTTCGT TCTGGTGCTG 
GTGCTGCTTC ACACCCACCT GAGCACAGAG ATGTTTGCAG CACCCATGGG CCGCTGCGAG 
GCTGGTGTTA TGCATCTGTT CTATATACGT GCGGGGGTGT CAAGGGCCAT GCTGCTGCGC 
CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGACCTCG ACTGCCCGTA CTTGGTTCAT 
GTGCCAGTGG TTGCCTTCCG CCTGGAACCC AGGAACCAGA GGGGAGTGTT TTCTGTGGAC 
GGGGAGCTGA TGGTGTGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG 
GTCTCTGGTA GCAGTGACTC CCCGTCCGGC CGGGACTCCC AGCGGAGGCC ACCTCCAGAA 
GAACCAATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sphk1 ( NM_133386.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_596877.2 Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant 6, mRNA.

Price & Availability↑

CloneID	ORa00646
Clone ID Related Accession (Same CDS sequence)	NM_001270808.1 , NM_133386.3 , NM_001270810.1 , NM_001270809.1 , NM_001270807.1
Accession Version	NM_133386.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Rattus norvegicus(Norway rat)
Product	sphingosine kinase 1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07030835.1 and BC081738.1. On Aug 2, 2012 this sequence version replaced NM_133386.2. Transcript Variant: This variant (6) retains an intron compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, 4, 5, and 6 all encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC081738.1, AB049575.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAACCAG CAGACTGTCC CCGGGGACTG CTCCCACGGC CATGCAGAGT GTTGGTGCTG 
CTGAACCCCC GGGGTGGCAA GGGCAAGGCT CTGAAGCTCT TTCAGAGCCG TGTGCGGCCC 
CTTCTGGAGG AGGCTGAGGT ATCCTTTAAA CTGATGCTCA CCGAACGGCA GAACCATGCC 
AGGGAGCTGG TGTGTGCAGA AGAACTGGGT CACTGGGATG CCTTGGCGGT CATGTCCGGT 
GACGGTCTGA TGCACGAGGT GGTGAATGGG CTAATGGAAC GACCTGACTG GGAGAGTGCC 
ATCCAGAAAC CCCTATGTAG CCTCCCTGGA GGCTCTGGCA ATGCGCTGGC AGCTTCTTTG 
AACTACTATG CTGGGCACGA GCAGGTCACT AATGAAGATC TCCTGATCAA CTGCACACTG 
CTGTTATGCT GCCGGCAGCT GTCACCCATG AATCTGCTGT CCCTGCACAC CGCTTCCGGA 
CGGCAACTCT ATTCTGTTCT CAGTCTGTCC TGGGGTTTCG TTGCTGACGT GGACTTGGAG 
AGTGAGAAAT ACAGGAGCTT GGGGGAGATT CGTTTCACGG TGGGAACCTT TTTTCGCCTA 
GCAAGCCTGC GCATTTACCA AGGCCAACTG GCCTACCTTC CTGTAGGGAA GGCTGCCTCT 
AAGATACCTG CTTCCTCACT GGCACAGAAG GGCCCTGCCA ACACATACCT CGTTCCTCTG 
GAGGAGCCAG TGCCACCTCA TTGGACTGTG GTGCCAGAGC AGGACTTCGT TCTGGTGCTG 
GTGCTGCTTC ACACCCACCT GAGCACAGAG ATGTTTGCAG CACCCATGGG CCGCTGCGAG 
GCTGGTGTTA TGCATCTGTT CTATATACGT GCGGGGGTGT CAAGGGCCAT GCTGCTGCGC 
CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGACCTCG ACTGCCCGTA CTTGGTTCAT 
GTGCCAGTGG TTGCCTTCCG CCTGGAACCC AGGAACCAGA GGGGAGTGTT TTCTGTGGAC 
GGGGAGCTGA TGGTGTGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG 
GTCTCTGGTA GCAGTGACTC CCCGTCCGGC CGGGACTCCC AGCGGAGGCC ACCTCCAGAA 
GAACCAATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_596877.2
CDS	1728..2879
Translation	MQPADCPRGLLPRPCRVLVLLNPRGGKGKALKLFQSRVRPLLEEAEVSFKLMLTERQNHARELVCAEELGHWDALAVMSGDGLMHEVVNGLMERPDWESAIQKPLCSLPGGSGNALAASLNYYAGHEQVTNEDLLINCTLLLCCRQLSPMNLLSLHTASGRQLYSVLSLSWGFVADVDLESEKYRSLGEIRFTVGTFFRLASLRIYQGQLAYLPVGKAASKIPASSLAQKGPANTYLVPLEEPVPPHWTVVPEQDFVLVLVLLHTHLSTEMFAAPMGRCEAGVMHLFYIRAGVSRAMLLRLFLAMQKGKHMDLDCPYLVHVPVVAFRLEPRNQRGVFSVDGELMVCEAVQGQVHPNYLWMVSGSSDSPSGRDSQRRPPPEEPI

Target ORF information:

RefSeq Version	NM_133386.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133386.3

ORF Insert Sequence:

ATGCAACCAG CAGACTGTCC CCGGGGACTG CTCCCACGGC CATGCAGAGT GTTGGTGCTG 
CTGAACCCCC GGGGTGGCAA GGGCAAGGCT CTGAAGCTCT TTCAGAGCCG TGTGCGGCCC 
CTTCTGGAGG AGGCTGAGGT ATCCTTTAAA CTGATGCTCA CCGAACGGCA GAACCATGCC 
AGGGAGCTGG TGTGTGCAGA AGAACTGGGT CACTGGGATG CCTTGGCGGT CATGTCCGGT 
GACGGTCTGA TGCACGAGGT GGTGAATGGG CTAATGGAAC GACCTGACTG GGAGAGTGCC 
ATCCAGAAAC CCCTATGTAG CCTCCCTGGA GGCTCTGGCA ATGCGCTGGC AGCTTCTTTG 
AACTACTATG CTGGGCACGA GCAGGTCACT AATGAAGATC TCCTGATCAA CTGCACACTG 
CTGTTATGCT GCCGGCAGCT GTCACCCATG AATCTGCTGT CCCTGCACAC CGCTTCCGGA 
CGGCAACTCT ATTCTGTTCT CAGTCTGTCC TGGGGTTTCG TTGCTGACGT GGACTTGGAG 
AGTGAGAAAT ACAGGAGCTT GGGGGAGATT CGTTTCACGG TGGGAACCTT TTTTCGCCTA 
GCAAGCCTGC GCATTTACCA AGGCCAACTG GCCTACCTTC CTGTAGGGAA GGCTGCCTCT 
AAGATACCTG CTTCCTCACT GGCACAGAAG GGCCCTGCCA ACACATACCT CGTTCCTCTG 
GAGGAGCCAG TGCCACCTCA TTGGACTGTG GTGCCAGAGC AGGACTTCGT TCTGGTGCTG 
GTGCTGCTTC ACACCCACCT GAGCACAGAG ATGTTTGCAG CACCCATGGG CCGCTGCGAG 
GCTGGTGTTA TGCATCTGTT CTATATACGT GCGGGGGTGT CAAGGGCCAT GCTGCTGCGC 
CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGACCTCG ACTGCCCGTA CTTGGTTCAT 
GTGCCAGTGG TTGCCTTCCG CCTGGAACCC AGGAACCAGA GGGGAGTGTT TTCTGTGGAC 
GGGGAGCTGA TGGTGTGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG 
GTCTCTGGTA GCAGTGACTC CCCGTCCGGC CGGGACTCCC AGCGGAGGCC ACCTCCAGAA 
GAACCAATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sphk1 ( NM_001270810.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001257739.1 Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	ORa00646
Clone ID Related Accession (Same CDS sequence)	NM_001270808.1 , NM_133386.3 , NM_001270810.1 , NM_001270809.1 , NM_001270807.1
Accession Version	NM_001270810.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Rattus norvegicus(Norway rat)
Product	sphingosine kinase 1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07030835.1 and BC081738.1. Transcript Variant: This variant (5) uses an alternate splice junction at the 3' end of the first exon compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, 4, 5, and 6 all encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB049574.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906347, SAMN01906349 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAACCAG CAGACTGTCC CCGGGGACTG CTCCCACGGC CATGCAGAGT GTTGGTGCTG 
CTGAACCCCC GGGGTGGCAA GGGCAAGGCT CTGAAGCTCT TTCAGAGCCG TGTGCGGCCC 
CTTCTGGAGG AGGCTGAGGT ATCCTTTAAA CTGATGCTCA CCGAACGGCA GAACCATGCC 
AGGGAGCTGG TGTGTGCAGA AGAACTGGGT CACTGGGATG CCTTGGCGGT CATGTCCGGT 
GACGGTCTGA TGCACGAGGT GGTGAATGGG CTAATGGAAC GACCTGACTG GGAGAGTGCC 
ATCCAGAAAC CCCTATGTAG CCTCCCTGGA GGCTCTGGCA ATGCGCTGGC AGCTTCTTTG 
AACTACTATG CTGGGCACGA GCAGGTCACT AATGAAGATC TCCTGATCAA CTGCACACTG 
CTGTTATGCT GCCGGCAGCT GTCACCCATG AATCTGCTGT CCCTGCACAC CGCTTCCGGA 
CGGCAACTCT ATTCTGTTCT CAGTCTGTCC TGGGGTTTCG TTGCTGACGT GGACTTGGAG 
AGTGAGAAAT ACAGGAGCTT GGGGGAGATT CGTTTCACGG TGGGAACCTT TTTTCGCCTA 
GCAAGCCTGC GCATTTACCA AGGCCAACTG GCCTACCTTC CTGTAGGGAA GGCTGCCTCT 
AAGATACCTG CTTCCTCACT GGCACAGAAG GGCCCTGCCA ACACATACCT CGTTCCTCTG 
GAGGAGCCAG TGCCACCTCA TTGGACTGTG GTGCCAGAGC AGGACTTCGT TCTGGTGCTG 
GTGCTGCTTC ACACCCACCT GAGCACAGAG ATGTTTGCAG CACCCATGGG CCGCTGCGAG 
GCTGGTGTTA TGCATCTGTT CTATATACGT GCGGGGGTGT CAAGGGCCAT GCTGCTGCGC 
CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGACCTCG ACTGCCCGTA CTTGGTTCAT 
GTGCCAGTGG TTGCCTTCCG CCTGGAACCC AGGAACCAGA GGGGAGTGTT TTCTGTGGAC 
GGGGAGCTGA TGGTGTGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG 
GTCTCTGGTA GCAGTGACTC CCCGTCCGGC CGGGACTCCC AGCGGAGGCC ACCTCCAGAA 
GAACCAATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257739.1
CDS	1124..2275
Translation	MQPADCPRGLLPRPCRVLVLLNPRGGKGKALKLFQSRVRPLLEEAEVSFKLMLTERQNHARELVCAEELGHWDALAVMSGDGLMHEVVNGLMERPDWESAIQKPLCSLPGGSGNALAASLNYYAGHEQVTNEDLLINCTLLLCCRQLSPMNLLSLHTASGRQLYSVLSLSWGFVADVDLESEKYRSLGEIRFTVGTFFRLASLRIYQGQLAYLPVGKAASKIPASSLAQKGPANTYLVPLEEPVPPHWTVVPEQDFVLVLVLLHTHLSTEMFAAPMGRCEAGVMHLFYIRAGVSRAMLLRLFLAMQKGKHMDLDCPYLVHVPVVAFRLEPRNQRGVFSVDGELMVCEAVQGQVHPNYLWMVSGSSDSPSGRDSQRRPPPEEPI

Target ORF information:

RefSeq Version	NM_001270810.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270810.1

ORF Insert Sequence:

ATGCAACCAG CAGACTGTCC CCGGGGACTG CTCCCACGGC CATGCAGAGT GTTGGTGCTG 
CTGAACCCCC GGGGTGGCAA GGGCAAGGCT CTGAAGCTCT TTCAGAGCCG TGTGCGGCCC 
CTTCTGGAGG AGGCTGAGGT ATCCTTTAAA CTGATGCTCA CCGAACGGCA GAACCATGCC 
AGGGAGCTGG TGTGTGCAGA AGAACTGGGT CACTGGGATG CCTTGGCGGT CATGTCCGGT 
GACGGTCTGA TGCACGAGGT GGTGAATGGG CTAATGGAAC GACCTGACTG GGAGAGTGCC 
ATCCAGAAAC CCCTATGTAG CCTCCCTGGA GGCTCTGGCA ATGCGCTGGC AGCTTCTTTG 
AACTACTATG CTGGGCACGA GCAGGTCACT AATGAAGATC TCCTGATCAA CTGCACACTG 
CTGTTATGCT GCCGGCAGCT GTCACCCATG AATCTGCTGT CCCTGCACAC CGCTTCCGGA 
CGGCAACTCT ATTCTGTTCT CAGTCTGTCC TGGGGTTTCG TTGCTGACGT GGACTTGGAG 
AGTGAGAAAT ACAGGAGCTT GGGGGAGATT CGTTTCACGG TGGGAACCTT TTTTCGCCTA 
GCAAGCCTGC GCATTTACCA AGGCCAACTG GCCTACCTTC CTGTAGGGAA GGCTGCCTCT 
AAGATACCTG CTTCCTCACT GGCACAGAAG GGCCCTGCCA ACACATACCT CGTTCCTCTG 
GAGGAGCCAG TGCCACCTCA TTGGACTGTG GTGCCAGAGC AGGACTTCGT TCTGGTGCTG 
GTGCTGCTTC ACACCCACCT GAGCACAGAG ATGTTTGCAG CACCCATGGG CCGCTGCGAG 
GCTGGTGTTA TGCATCTGTT CTATATACGT GCGGGGGTGT CAAGGGCCAT GCTGCTGCGC 
CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGACCTCG ACTGCCCGTA CTTGGTTCAT 
GTGCCAGTGG TTGCCTTCCG CCTGGAACCC AGGAACCAGA GGGGAGTGTT TTCTGTGGAC 
GGGGAGCTGA TGGTGTGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG 
GTCTCTGGTA GCAGTGACTC CCCGTCCGGC CGGGACTCCC AGCGGAGGCC ACCTCCAGAA 
GAACCAATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sphk1 ( NM_001270809.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001257738.1 Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant 4, mRNA.

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CloneID	ORa00646
Clone ID Related Accession (Same CDS sequence)	NM_001270808.1 , NM_133386.3 , NM_001270810.1 , NM_001270809.1 , NM_001270807.1
Accession Version	NM_001270809.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Rattus norvegicus(Norway rat)
Product	sphingosine kinase 1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07030835.1 and BC081738.1. Transcript Variant: This variant (4) uses an alternate splice junction at the 3' end of the first exon compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, 4, 5, and 6 all encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB049573.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAACCAG CAGACTGTCC CCGGGGACTG CTCCCACGGC CATGCAGAGT GTTGGTGCTG 
CTGAACCCCC GGGGTGGCAA GGGCAAGGCT CTGAAGCTCT TTCAGAGCCG TGTGCGGCCC 
CTTCTGGAGG AGGCTGAGGT ATCCTTTAAA CTGATGCTCA CCGAACGGCA GAACCATGCC 
AGGGAGCTGG TGTGTGCAGA AGAACTGGGT CACTGGGATG CCTTGGCGGT CATGTCCGGT 
GACGGTCTGA TGCACGAGGT GGTGAATGGG CTAATGGAAC GACCTGACTG GGAGAGTGCC 
ATCCAGAAAC CCCTATGTAG CCTCCCTGGA GGCTCTGGCA ATGCGCTGGC AGCTTCTTTG 
AACTACTATG CTGGGCACGA GCAGGTCACT AATGAAGATC TCCTGATCAA CTGCACACTG 
CTGTTATGCT GCCGGCAGCT GTCACCCATG AATCTGCTGT CCCTGCACAC CGCTTCCGGA 
CGGCAACTCT ATTCTGTTCT CAGTCTGTCC TGGGGTTTCG TTGCTGACGT GGACTTGGAG 
AGTGAGAAAT ACAGGAGCTT GGGGGAGATT CGTTTCACGG TGGGAACCTT TTTTCGCCTA 
GCAAGCCTGC GCATTTACCA AGGCCAACTG GCCTACCTTC CTGTAGGGAA GGCTGCCTCT 
AAGATACCTG CTTCCTCACT GGCACAGAAG GGCCCTGCCA ACACATACCT CGTTCCTCTG 
GAGGAGCCAG TGCCACCTCA TTGGACTGTG GTGCCAGAGC AGGACTTCGT TCTGGTGCTG 
GTGCTGCTTC ACACCCACCT GAGCACAGAG ATGTTTGCAG CACCCATGGG CCGCTGCGAG 
GCTGGTGTTA TGCATCTGTT CTATATACGT GCGGGGGTGT CAAGGGCCAT GCTGCTGCGC 
CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGACCTCG ACTGCCCGTA CTTGGTTCAT 
GTGCCAGTGG TTGCCTTCCG CCTGGAACCC AGGAACCAGA GGGGAGTGTT TTCTGTGGAC 
GGGGAGCTGA TGGTGTGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG 
GTCTCTGGTA GCAGTGACTC CCCGTCCGGC CGGGACTCCC AGCGGAGGCC ACCTCCAGAA 
GAACCAATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257738.1
CDS	438..1589
Translation	MQPADCPRGLLPRPCRVLVLLNPRGGKGKALKLFQSRVRPLLEEAEVSFKLMLTERQNHARELVCAEELGHWDALAVMSGDGLMHEVVNGLMERPDWESAIQKPLCSLPGGSGNALAASLNYYAGHEQVTNEDLLINCTLLLCCRQLSPMNLLSLHTASGRQLYSVLSLSWGFVADVDLESEKYRSLGEIRFTVGTFFRLASLRIYQGQLAYLPVGKAASKIPASSLAQKGPANTYLVPLEEPVPPHWTVVPEQDFVLVLVLLHTHLSTEMFAAPMGRCEAGVMHLFYIRAGVSRAMLLRLFLAMQKGKHMDLDCPYLVHVPVVAFRLEPRNQRGVFSVDGELMVCEAVQGQVHPNYLWMVSGSSDSPSGRDSQRRPPPEEPI

Target ORF information:

RefSeq Version	NM_001270809.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270809.1

ORF Insert Sequence:

ATGCAACCAG CAGACTGTCC CCGGGGACTG CTCCCACGGC CATGCAGAGT GTTGGTGCTG 
CTGAACCCCC GGGGTGGCAA GGGCAAGGCT CTGAAGCTCT TTCAGAGCCG TGTGCGGCCC 
CTTCTGGAGG AGGCTGAGGT ATCCTTTAAA CTGATGCTCA CCGAACGGCA GAACCATGCC 
AGGGAGCTGG TGTGTGCAGA AGAACTGGGT CACTGGGATG CCTTGGCGGT CATGTCCGGT 
GACGGTCTGA TGCACGAGGT GGTGAATGGG CTAATGGAAC GACCTGACTG GGAGAGTGCC 
ATCCAGAAAC CCCTATGTAG CCTCCCTGGA GGCTCTGGCA ATGCGCTGGC AGCTTCTTTG 
AACTACTATG CTGGGCACGA GCAGGTCACT AATGAAGATC TCCTGATCAA CTGCACACTG 
CTGTTATGCT GCCGGCAGCT GTCACCCATG AATCTGCTGT CCCTGCACAC CGCTTCCGGA 
CGGCAACTCT ATTCTGTTCT CAGTCTGTCC TGGGGTTTCG TTGCTGACGT GGACTTGGAG 
AGTGAGAAAT ACAGGAGCTT GGGGGAGATT CGTTTCACGG TGGGAACCTT TTTTCGCCTA 
GCAAGCCTGC GCATTTACCA AGGCCAACTG GCCTACCTTC CTGTAGGGAA GGCTGCCTCT 
AAGATACCTG CTTCCTCACT GGCACAGAAG GGCCCTGCCA ACACATACCT CGTTCCTCTG 
GAGGAGCCAG TGCCACCTCA TTGGACTGTG GTGCCAGAGC AGGACTTCGT TCTGGTGCTG 
GTGCTGCTTC ACACCCACCT GAGCACAGAG ATGTTTGCAG CACCCATGGG CCGCTGCGAG 
GCTGGTGTTA TGCATCTGTT CTATATACGT GCGGGGGTGT CAAGGGCCAT GCTGCTGCGC 
CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGACCTCG ACTGCCCGTA CTTGGTTCAT 
GTGCCAGTGG TTGCCTTCCG CCTGGAACCC AGGAACCAGA GGGGAGTGTT TTCTGTGGAC 
GGGGAGCTGA TGGTGTGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG 
GTCTCTGGTA GCAGTGACTC CCCGTCCGGC CGGGACTCCC AGCGGAGGCC ACCTCCAGAA 
GAACCAATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sphk1 ( NM_001270807.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001257736.1 Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant 2, mRNA.

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CloneID	ORa00646
Clone ID Related Accession (Same CDS sequence)	NM_001270808.1 , NM_133386.3 , NM_001270810.1 , NM_001270809.1 , NM_001270807.1
Accession Version	NM_001270807.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Rattus norvegicus(Norway rat)
Product	sphingosine kinase 1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07030835.1 and BC081738.1. Transcript Variant: This variant (2) uses an alternate first exon compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, 4, 5, and 6 all encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB049572.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAACCAG CAGACTGTCC CCGGGGACTG CTCCCACGGC CATGCAGAGT GTTGGTGCTG 
CTGAACCCCC GGGGTGGCAA GGGCAAGGCT CTGAAGCTCT TTCAGAGCCG TGTGCGGCCC 
CTTCTGGAGG AGGCTGAGGT ATCCTTTAAA CTGATGCTCA CCGAACGGCA GAACCATGCC 
AGGGAGCTGG TGTGTGCAGA AGAACTGGGT CACTGGGATG CCTTGGCGGT CATGTCCGGT 
GACGGTCTGA TGCACGAGGT GGTGAATGGG CTAATGGAAC GACCTGACTG GGAGAGTGCC 
ATCCAGAAAC CCCTATGTAG CCTCCCTGGA GGCTCTGGCA ATGCGCTGGC AGCTTCTTTG 
AACTACTATG CTGGGCACGA GCAGGTCACT AATGAAGATC TCCTGATCAA CTGCACACTG 
CTGTTATGCT GCCGGCAGCT GTCACCCATG AATCTGCTGT CCCTGCACAC CGCTTCCGGA 
CGGCAACTCT ATTCTGTTCT CAGTCTGTCC TGGGGTTTCG TTGCTGACGT GGACTTGGAG 
AGTGAGAAAT ACAGGAGCTT GGGGGAGATT CGTTTCACGG TGGGAACCTT TTTTCGCCTA 
GCAAGCCTGC GCATTTACCA AGGCCAACTG GCCTACCTTC CTGTAGGGAA GGCTGCCTCT 
AAGATACCTG CTTCCTCACT GGCACAGAAG GGCCCTGCCA ACACATACCT CGTTCCTCTG 
GAGGAGCCAG TGCCACCTCA TTGGACTGTG GTGCCAGAGC AGGACTTCGT TCTGGTGCTG 
GTGCTGCTTC ACACCCACCT GAGCACAGAG ATGTTTGCAG CACCCATGGG CCGCTGCGAG 
GCTGGTGTTA TGCATCTGTT CTATATACGT GCGGGGGTGT CAAGGGCCAT GCTGCTGCGC 
CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGACCTCG ACTGCCCGTA CTTGGTTCAT 
GTGCCAGTGG TTGCCTTCCG CCTGGAACCC AGGAACCAGA GGGGAGTGTT TTCTGTGGAC 
GGGGAGCTGA TGGTGTGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG 
GTCTCTGGTA GCAGTGACTC CCCGTCCGGC CGGGACTCCC AGCGGAGGCC ACCTCCAGAA 
GAACCAATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257736.1
CDS	488..1639
Translation	MQPADCPRGLLPRPCRVLVLLNPRGGKGKALKLFQSRVRPLLEEAEVSFKLMLTERQNHARELVCAEELGHWDALAVMSGDGLMHEVVNGLMERPDWESAIQKPLCSLPGGSGNALAASLNYYAGHEQVTNEDLLINCTLLLCCRQLSPMNLLSLHTASGRQLYSVLSLSWGFVADVDLESEKYRSLGEIRFTVGTFFRLASLRIYQGQLAYLPVGKAASKIPASSLAQKGPANTYLVPLEEPVPPHWTVVPEQDFVLVLVLLHTHLSTEMFAAPMGRCEAGVMHLFYIRAGVSRAMLLRLFLAMQKGKHMDLDCPYLVHVPVVAFRLEPRNQRGVFSVDGELMVCEAVQGQVHPNYLWMVSGSSDSPSGRDSQRRPPPEEPI

Target ORF information:

RefSeq Version	NM_001270807.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270807.1

ORF Insert Sequence:

ATGCAACCAG CAGACTGTCC CCGGGGACTG CTCCCACGGC CATGCAGAGT GTTGGTGCTG 
CTGAACCCCC GGGGTGGCAA GGGCAAGGCT CTGAAGCTCT TTCAGAGCCG TGTGCGGCCC 
CTTCTGGAGG AGGCTGAGGT ATCCTTTAAA CTGATGCTCA CCGAACGGCA GAACCATGCC 
AGGGAGCTGG TGTGTGCAGA AGAACTGGGT CACTGGGATG CCTTGGCGGT CATGTCCGGT 
GACGGTCTGA TGCACGAGGT GGTGAATGGG CTAATGGAAC GACCTGACTG GGAGAGTGCC 
ATCCAGAAAC CCCTATGTAG CCTCCCTGGA GGCTCTGGCA ATGCGCTGGC AGCTTCTTTG 
AACTACTATG CTGGGCACGA GCAGGTCACT AATGAAGATC TCCTGATCAA CTGCACACTG 
CTGTTATGCT GCCGGCAGCT GTCACCCATG AATCTGCTGT CCCTGCACAC CGCTTCCGGA 
CGGCAACTCT ATTCTGTTCT CAGTCTGTCC TGGGGTTTCG TTGCTGACGT GGACTTGGAG 
AGTGAGAAAT ACAGGAGCTT GGGGGAGATT CGTTTCACGG TGGGAACCTT TTTTCGCCTA 
GCAAGCCTGC GCATTTACCA AGGCCAACTG GCCTACCTTC CTGTAGGGAA GGCTGCCTCT 
AAGATACCTG CTTCCTCACT GGCACAGAAG GGCCCTGCCA ACACATACCT CGTTCCTCTG 
GAGGAGCCAG TGCCACCTCA TTGGACTGTG GTGCCAGAGC AGGACTTCGT TCTGGTGCTG 
GTGCTGCTTC ACACCCACCT GAGCACAGAG ATGTTTGCAG CACCCATGGG CCGCTGCGAG 
GCTGGTGTTA TGCATCTGTT CTATATACGT GCGGGGGTGT CAAGGGCCAT GCTGCTGCGC 
CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGACCTCG ACTGCCCGTA CTTGGTTCAT 
GTGCCAGTGG TTGCCTTCCG CCTGGAACCC AGGAACCAGA GGGGAGTGTT TTCTGTGGAC 
GGGGAGCTGA TGGTGTGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG 
GTCTCTGGTA GCAGTGACTC CCCGTCCGGC CGGGACTCCC AGCGGAGGCC ACCTCCAGAA 
GAACCAATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sphk1 ( NM_001270811.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001257740.1 Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant 1, mRNA.

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CloneID	ORa09850
Clone ID Related Accession (Same CDS sequence)	NM_001270811.1
Accession Version	NM_001270811.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Rattus norvegicus(Norway rat)
Product	sphingosine kinase 1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07030835.1 and BC081738.1. Transcript Variant: This variant (1) encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ486894.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAACTGG ACTCTCCACC AACCTCAAAG GATCTGGCCG CCTCCGGGAA TGACGAGGGC 
GCTCTCACAG CCCAGGGAGG TGGAGAGGGC GAGCCCCACG GCCAGTCACC AGACACCCGC 
CTGGGCAACA CCGATAAGCA GCTGCACACT GGAGCCGCCG TTACCTCTAG CAGCGCCGGG 
GCAGCATCGG TGGCTCCTTG TCAGCGGGAG CCCCGGGACC TGGCTATGCA ACCAGCAGAC 
TGTCCCCGGG GACTGCTCCC ACGGCCATGC AGAGTGTTGG TGCTGCTGAA CCCCCGGGGT 
GGCAAGGGCA AGGCTCTGAA GCTCTTTCAG AGCCGTGTGC GGCCCCTTCT GGAGGAGGCT 
GAGGTATCCT TTAAACTGAT GCTCACCGAA CGGCAGAACC ATGCCAGGGA GCTGGTGTGT 
GCAGAAGAAC TGGGTCACTG GGATGCCTTG GCGGTCATGT CCGGTGACGG TCTGATGCAC 
GAGGTGGTGA ATGGGCTAAT GGAACGACCT GACTGGGAGA GTGCCATCCA GAAACCCCTA 
TGTAGCCTCC CTGGAGGCTC TGGCAATGCG CTGGCAGCTT CTTTGAACTA CTATGCTGGG 
CACGAGCAGG TCACTAATGA AGATCTCCTG ATCAACTGCA CACTGCTGTT ATGCTGCCGG 
CAGCTGTCAC CCATGAATCT GCTGTCCCTG CACACCGCTT CCGGACGGCA ACTCTATTCT 
GTTCTCAGTC TGTCCTGGGG TTTCGTTGCT GACGTGGACT TGGAGAGTGA GAAATACAGG 
AGCTTGGGGG AGATTCGTTT CACGGTGGGA ACCTTTTTTC GCCTAGCAAG CCTGCGCATT 
TACCAAGGCC AACTGGCCTA CCTTCCTGTA GGGAAGGCTG CCTCTAAGAT ACCTGCTTCC 
TCACTGGCAC AGAAGGGCCC TGCCAACACA TACCTCGTTC CTCTGGAGGA GCCAGTGCCA 
CCTCATTGGA CTGTGGTGCC AGAGCAGGAC TTCGTTCTGG TGCTGGTGCT GCTTCACACC 
CACCTGAGCA CAGAGATGTT TGCAGCACCC ATGGGCCGCT GCGAGGCTGG TGTTATGCAT 
CTGTTCTATA TACGTGCGGG GGTGTCAAGG GCCATGCTGC TGCGCCTCTT CCTGGCCATG 
CAGAAGGGCA AGCATATGGA CCTCGACTGC CCGTACTTGG TTCATGTGCC AGTGGTTGCC 
TTCCGCCTGG AACCCAGGAA CCAGAGGGGA GTGTTTTCTG TGGACGGGGA GCTGATGGTG 
TGTGAAGCTG TGCAGGGCCA AGTGCACCCA AACTACCTTT GGATGGTCTC TGGTAGCAGT 
GACTCCCCGT CCGGCCGGGA CTCCCAGCGG AGGCCACCTC CAGAAGAACC AATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257740.1
CDS	1249..2625
Translation	MQLDSPPTSKDLAASGNDEGALTAQGGGEGEPHGQSPDTRLGNTDKQLHTGAAVTSSSAGAASVAPCQREPRDLAMQPADCPRGLLPRPCRVLVLLNPRGGKGKALKLFQSRVRPLLEEAEVSFKLMLTERQNHARELVCAEELGHWDALAVMSGDGLMHEVVNGLMERPDWESAIQKPLCSLPGGSGNALAASLNYYAGHEQVTNEDLLINCTLLLCCRQLSPMNLLSLHTASGRQLYSVLSLSWGFVADVDLESEKYRSLGEIRFTVGTFFRLASLRIYQGQLAYLPVGKAASKIPASSLAQKGPANTYLVPLEEPVPPHWTVVPEQDFVLVLVLLHTHLSTEMFAAPMGRCEAGVMHLFYIRAGVSRAMLLRLFLAMQKGKHMDLDCPYLVHVPVVAFRLEPRNQRGVFSVDGELMVCEAVQGQVHPNYLWMVSGSSDSPSGRDSQRRPPPEEPI

Target ORF information:

RefSeq Version	NM_001270811.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270811.1

ORF Insert Sequence:

ATGCAACTGG ACTCTCCACC AACCTCAAAG GATCTGGCCG CCTCCGGGAA TGACGAGGGC 
GCTCTCACAG CCCAGGGAGG TGGAGAGGGC GAGCCCCACG GCCAGTCACC AGACACCCGC 
CTGGGCAACA CCGATAAGCA GCTGCACACT GGAGCCGCCG TTACCTCTAG CAGCGCCGGG 
GCAGCATCGG TGGCTCCTTG TCAGCGGGAG CCCCGGGACC TGGCTATGCA ACCAGCAGAC 
TGTCCCCGGG GACTGCTCCC ACGGCCATGC AGAGTGTTGG TGCTGCTGAA CCCCCGGGGT 
GGCAAGGGCA AGGCTCTGAA GCTCTTTCAG AGCCGTGTGC GGCCCCTTCT GGAGGAGGCT 
GAGGTATCCT TTAAACTGAT GCTCACCGAA CGGCAGAACC ATGCCAGGGA GCTGGTGTGT 
GCAGAAGAAC TGGGTCACTG GGATGCCTTG GCGGTCATGT CCGGTGACGG TCTGATGCAC 
GAGGTGGTGA ATGGGCTAAT GGAACGACCT GACTGGGAGA GTGCCATCCA GAAACCCCTA 
TGTAGCCTCC CTGGAGGCTC TGGCAATGCG CTGGCAGCTT CTTTGAACTA CTATGCTGGG 
CACGAGCAGG TCACTAATGA AGATCTCCTG ATCAACTGCA CACTGCTGTT ATGCTGCCGG 
CAGCTGTCAC CCATGAATCT GCTGTCCCTG CACACCGCTT CCGGACGGCA ACTCTATTCT 
GTTCTCAGTC TGTCCTGGGG TTTCGTTGCT GACGTGGACT TGGAGAGTGA GAAATACAGG 
AGCTTGGGGG AGATTCGTTT CACGGTGGGA ACCTTTTTTC GCCTAGCAAG CCTGCGCATT 
TACCAAGGCC AACTGGCCTA CCTTCCTGTA GGGAAGGCTG CCTCTAAGAT ACCTGCTTCC 
TCACTGGCAC AGAAGGGCCC TGCCAACACA TACCTCGTTC CTCTGGAGGA GCCAGTGCCA 
CCTCATTGGA CTGTGGTGCC AGAGCAGGAC TTCGTTCTGG TGCTGGTGCT GCTTCACACC 
CACCTGAGCA CAGAGATGTT TGCAGCACCC ATGGGCCGCT GCGAGGCTGG TGTTATGCAT 
CTGTTCTATA TACGTGCGGG GGTGTCAAGG GCCATGCTGC TGCGCCTCTT CCTGGCCATG 
CAGAAGGGCA AGCATATGGA CCTCGACTGC CCGTACTTGG TTCATGTGCC AGTGGTTGCC 
TTCCGCCTGG AACCCAGGAA CCAGAGGGGA GTGTTTTCTG TGGACGGGGA GCTGATGGTG 
TGTGAAGCTG TGCAGGGCCA AGTGCACCCA AACTACCTTT GGATGGTCTC TGGTAGCAGT 
GACTCCCCGT CCGGCCGGGA CTCCCAGCGG AGGCCACCTC CAGAAGAACC AATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sphk1 ( XM_006247787.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247849.1 Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant X1, mRNA.

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CloneID	ORa33963
Clone ID Related Accession (Same CDS sequence)	XM_006247787.2
Accession Version	XM_006247787.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sphingosine kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006247787.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACCAG ACTGTCCCCG GGGACTGCTC CCACGGCCAT GCAGAGTGTT GGTGCTGCTG 
AACCCCCGGG GTGGCAAGGG CAAGGCTCTG AAGCTCTTTC AGAGCCGTGT GCGGCCCCTT 
CTGGAGGAGG CTGAGGTATC CTTTAAACTG ATGCTCACCG AACGGCAGAA CCATGCCAGG 
GAGCTGGTGT GTGCAGAAGA ACTGGGTCAC TGGGATGCCT TGGCGGTCAT GTCCGGTGAC 
GGTCTGATGC ACGAGGTGGT GAATGGGCTA ATGGAACGAC CTGACTGGGA GAGTGCCATC 
CAGAAACCCC TATGTAGCCT CCCTGGAGGC TCTGGCAATG CGCTGGCAGC TTCTTTGAAC 
TACTATGCTG GGCACGAGCA GGTCACTAAT GAAGATCTCC TGATCAACTG CACACTGCTG 
TTATGCTGCC GGCAGCTGTC ACCCATGAAT CTGCTGTCCC TGCACACCGC TTCCGGACGG 
CAACTCTATT CTGTTCTCAG TCTGTCCTGG GGTTTCGTTG CTGACGTGGA CTTGGAGAGT 
GAGAAATACA GGAGCTTGGG GGAGATTCGT TTCACGGTGG GAACCTTTTT TCGCCTAGCA 
AGCCTGCGCA TTTACCAAGG CCAACTGGCC TACCTTCCTG TAGGGAAGGC TGCCTCTAAG 
ATACCTGCTT CCTCACTGGC ACAGAAGGGC CCTGCCAACA CATACCTCGT TCCTCTGGAG 
GAGCCAGTGC CACCTCATTG GACTGTGGTG CCAGAGCAGG ACTTCGTTCT GGTGCTGGTG 
CTGCTTCACA CCCACCTGAG CACAGAGATG TTTGCAGCAC CCATGGGCCG CTGCGAGGCT 
GGTGTTATGC ATCTGTTCTA TATACGTGCG GGGGTGTCAA GGGCCATGCT GCTGCGCCTC 
TTCCTGGCCA TGCAGAAGGG CAAGCATATG GACCTCGACT GCCCGTACTT GGTTCATGTG 
CCAGTGGTTG CCTTCCGCCT GGAACCCAGG AACCAGAGGG GAGTGTTTTC TGTGGACGGG 
GAGCTGATGG TGTGTGAAGC TGTGCAGGGC CAAGTGCACC CAAACTACCT TTGGATGGTC 
TCTGGTAGCA GTGACTCCCC GTCCGGCCGG GACTCCCAGC GGAGGCCACC TCCAGAAGAA 
CCAATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247849.1
CDS	811..1959
Translation	MQPDCPRGLLPRPCRVLVLLNPRGGKGKALKLFQSRVRPLLEEAEVSFKLMLTERQNHARELVCAEELGHWDALAVMSGDGLMHEVVNGLMERPDWESAIQKPLCSLPGGSGNALAASLNYYAGHEQVTNEDLLINCTLLLCCRQLSPMNLLSLHTASGRQLYSVLSLSWGFVADVDLESEKYRSLGEIRFTVGTFFRLASLRIYQGQLAYLPVGKAASKIPASSLAQKGPANTYLVPLEEPVPPHWTVVPEQDFVLVLVLLHTHLSTEMFAAPMGRCEAGVMHLFYIRAGVSRAMLLRLFLAMQKGKHMDLDCPYLVHVPVVAFRLEPRNQRGVFSVDGELMVCEAVQGQVHPNYLWMVSGSSDSPSGRDSQRRPPPEEPI

Target ORF information:

RefSeq Version	XM_006247787.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247787.2

ORF Insert Sequence:

ATGCAACCAG ACTGTCCCCG GGGACTGCTC CCACGGCCAT GCAGAGTGTT GGTGCTGCTG 
AACCCCCGGG GTGGCAAGGG CAAGGCTCTG AAGCTCTTTC AGAGCCGTGT GCGGCCCCTT 
CTGGAGGAGG CTGAGGTATC CTTTAAACTG ATGCTCACCG AACGGCAGAA CCATGCCAGG 
GAGCTGGTGT GTGCAGAAGA ACTGGGTCAC TGGGATGCCT TGGCGGTCAT GTCCGGTGAC 
GGTCTGATGC ACGAGGTGGT GAATGGGCTA ATGGAACGAC CTGACTGGGA GAGTGCCATC 
CAGAAACCCC TATGTAGCCT CCCTGGAGGC TCTGGCAATG CGCTGGCAGC TTCTTTGAAC 
TACTATGCTG GGCACGAGCA GGTCACTAAT GAAGATCTCC TGATCAACTG CACACTGCTG 
TTATGCTGCC GGCAGCTGTC ACCCATGAAT CTGCTGTCCC TGCACACCGC TTCCGGACGG 
CAACTCTATT CTGTTCTCAG TCTGTCCTGG GGTTTCGTTG CTGACGTGGA CTTGGAGAGT 
GAGAAATACA GGAGCTTGGG GGAGATTCGT TTCACGGTGG GAACCTTTTT TCGCCTAGCA 
AGCCTGCGCA TTTACCAAGG CCAACTGGCC TACCTTCCTG TAGGGAAGGC TGCCTCTAAG 
ATACCTGCTT CCTCACTGGC ACAGAAGGGC CCTGCCAACA CATACCTCGT TCCTCTGGAG 
GAGCCAGTGC CACCTCATTG GACTGTGGTG CCAGAGCAGG ACTTCGTTCT GGTGCTGGTG 
CTGCTTCACA CCCACCTGAG CACAGAGATG TTTGCAGCAC CCATGGGCCG CTGCGAGGCT 
GGTGTTATGC ATCTGTTCTA TATACGTGCG GGGGTGTCAA GGGCCATGCT GCTGCGCCTC 
TTCCTGGCCA TGCAGAAGGG CAAGCATATG GACCTCGACT GCCCGTACTT GGTTCATGTG 
CCAGTGGTTG CCTTCCGCCT GGAACCCAGG AACCAGAGGG GAGTGTTTTC TGTGGACGGG 
GAGCTGATGG TGTGTGAAGC TGTGCAGGGC CAAGTGCACC CAAACTACCT TTGGATGGTC 
TCTGGTAGCA GTGACTCCCC GTCCGGCCGG GACTCCCAGC GGAGGCCACC TCCAGAAGAA 
CCAATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Extracellular vesicles from human-induced pluripotent stem cell-derived mesenchymal stromal cells (hiPSC-MSCs) protect against renal ischemia/reperfusion injury via delivering specificity protein (SP1) and transcriptional activating of sphingosine kinase 1 and inhibiting necroptosis.
Cell death & disease8(12)3200(2017 Dec)
Yuan X,Li D,Chen X,Han C,Xu L,Huang T,Dong Z,Zhang M

Sphk1 mediates neuroinflammation and neuronal injury via TRAF2/NF-?B pathways in activated microglia in cerebral ischemia reperfusion.
Journal of neuroimmunology30535-41(2017 Apr)
Su D,Cheng Y,Li S,Dai D,Zhang W,Lv M

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Sphk1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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