Sphk1 cDNA ORF clone, Rattus norvegicus(Norway rat)

The following Sphk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sphk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa00646 NM_001270809.1
Latest version!
Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $223.30
$319.00
ORa00646 NM_001270810.1
Latest version!
Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $223.30
$319.00
ORa00646 NM_133386.3
Latest version!
Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $223.30
$319.00
ORa00646 NM_001270807.1
Latest version!
Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $223.30
$319.00
ORa00646 NM_001270808.1
Latest version!
Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $223.30
$319.00
ORa09850 NM_001270811.1
Latest version!
Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
ORa33963 XM_006247787.2
Latest version!
Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ORa00646
    Clone ID Related Accession (Same CDS sequence) NM_001270808.1 , NM_133386.3 , NM_001270810.1 , NM_001270809.1 , NM_001270807.1
    Accession Version NM_001270808.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1152bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-10
    Organism Rattus norvegicus(Norway rat)
    Product sphingosine kinase 1 isoform b
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07030835.1 and BC081738.1. Transcript Variant: This variant (3) uses an alternate first exon compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, 4, 5, and 6 all encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB049571.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCAACCAG CAGACTGTCC CCGGGGACTG CTCCCACGGC CATGCAGAGT GTTGGTGCTG 
    CTGAACCCCC GGGGTGGCAA GGGCAAGGCT CTGAAGCTCT TTCAGAGCCG TGTGCGGCCC
    CTTCTGGAGG AGGCTGAGGT ATCCTTTAAA CTGATGCTCA CCGAACGGCA GAACCATGCC
    AGGGAGCTGG TGTGTGCAGA AGAACTGGGT CACTGGGATG CCTTGGCGGT CATGTCCGGT
    GACGGTCTGA TGCACGAGGT GGTGAATGGG CTAATGGAAC GACCTGACTG GGAGAGTGCC
    ATCCAGAAAC CCCTATGTAG CCTCCCTGGA GGCTCTGGCA ATGCGCTGGC AGCTTCTTTG
    AACTACTATG CTGGGCACGA GCAGGTCACT AATGAAGATC TCCTGATCAA CTGCACACTG
    CTGTTATGCT GCCGGCAGCT GTCACCCATG AATCTGCTGT CCCTGCACAC CGCTTCCGGA
    CGGCAACTCT ATTCTGTTCT CAGTCTGTCC TGGGGTTTCG TTGCTGACGT GGACTTGGAG
    AGTGAGAAAT ACAGGAGCTT GGGGGAGATT CGTTTCACGG TGGGAACCTT TTTTCGCCTA
    GCAAGCCTGC GCATTTACCA AGGCCAACTG GCCTACCTTC CTGTAGGGAA GGCTGCCTCT
    AAGATACCTG CTTCCTCACT GGCACAGAAG GGCCCTGCCA ACACATACCT CGTTCCTCTG
    GAGGAGCCAG TGCCACCTCA TTGGACTGTG GTGCCAGAGC AGGACTTCGT TCTGGTGCTG
    GTGCTGCTTC ACACCCACCT GAGCACAGAG ATGTTTGCAG CACCCATGGG CCGCTGCGAG
    GCTGGTGTTA TGCATCTGTT CTATATACGT GCGGGGGTGT CAAGGGCCAT GCTGCTGCGC
    CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGACCTCG ACTGCCCGTA CTTGGTTCAT
    GTGCCAGTGG TTGCCTTCCG CCTGGAACCC AGGAACCAGA GGGGAGTGTT TTCTGTGGAC
    GGGGAGCTGA TGGTGTGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG
    GTCTCTGGTA GCAGTGACTC CCCGTCCGGC CGGGACTCCC AGCGGAGGCC ACCTCCAGAA
    GAACCAATAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001257737.1
    CDS537..1688
    Translation

    Target ORF information:

    RefSeq Version NM_001270808.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001270808.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCAACCAG CAGACTGTCC CCGGGGACTG CTCCCACGGC CATGCAGAGT GTTGGTGCTG 
    CTGAACCCCC GGGGTGGCAA GGGCAAGGCT CTGAAGCTCT TTCAGAGCCG TGTGCGGCCC
    CTTCTGGAGG AGGCTGAGGT ATCCTTTAAA CTGATGCTCA CCGAACGGCA GAACCATGCC
    AGGGAGCTGG TGTGTGCAGA AGAACTGGGT CACTGGGATG CCTTGGCGGT CATGTCCGGT
    GACGGTCTGA TGCACGAGGT GGTGAATGGG CTAATGGAAC GACCTGACTG GGAGAGTGCC
    ATCCAGAAAC CCCTATGTAG CCTCCCTGGA GGCTCTGGCA ATGCGCTGGC AGCTTCTTTG
    AACTACTATG CTGGGCACGA GCAGGTCACT AATGAAGATC TCCTGATCAA CTGCACACTG
    CTGTTATGCT GCCGGCAGCT GTCACCCATG AATCTGCTGT CCCTGCACAC CGCTTCCGGA
    CGGCAACTCT ATTCTGTTCT CAGTCTGTCC TGGGGTTTCG TTGCTGACGT GGACTTGGAG
    AGTGAGAAAT ACAGGAGCTT GGGGGAGATT CGTTTCACGG TGGGAACCTT TTTTCGCCTA
    GCAAGCCTGC GCATTTACCA AGGCCAACTG GCCTACCTTC CTGTAGGGAA GGCTGCCTCT
    AAGATACCTG CTTCCTCACT GGCACAGAAG GGCCCTGCCA ACACATACCT CGTTCCTCTG
    GAGGAGCCAG TGCCACCTCA TTGGACTGTG GTGCCAGAGC AGGACTTCGT TCTGGTGCTG
    GTGCTGCTTC ACACCCACCT GAGCACAGAG ATGTTTGCAG CACCCATGGG CCGCTGCGAG
    GCTGGTGTTA TGCATCTGTT CTATATACGT GCGGGGGTGT CAAGGGCCAT GCTGCTGCGC
    CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGACCTCG ACTGCCCGTA CTTGGTTCAT
    GTGCCAGTGG TTGCCTTCCG CCTGGAACCC AGGAACCAGA GGGGAGTGTT TTCTGTGGAC
    GGGGAGCTGA TGGTGTGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG
    GTCTCTGGTA GCAGTGACTC CCCGTCCGGC CGGGACTCCC AGCGGAGGCC ACCTCCAGAA
    GAACCAATAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa00646
    Clone ID Related Accession (Same CDS sequence) NM_001270808.1 , NM_133386.3 , NM_001270810.1 , NM_001270809.1 , NM_001270807.1
    Accession Version NM_133386.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1152bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-10
    Organism Rattus norvegicus(Norway rat)
    Product sphingosine kinase 1 isoform b
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07030835.1 and BC081738.1. On Aug 2, 2012 this sequence version replaced NM_133386.2. Transcript Variant: This variant (6) retains an intron compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, 4, 5, and 6 all encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC081738.1, AB049575.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCAACCAG CAGACTGTCC CCGGGGACTG CTCCCACGGC CATGCAGAGT GTTGGTGCTG 
    CTGAACCCCC GGGGTGGCAA GGGCAAGGCT CTGAAGCTCT TTCAGAGCCG TGTGCGGCCC
    CTTCTGGAGG AGGCTGAGGT ATCCTTTAAA CTGATGCTCA CCGAACGGCA GAACCATGCC
    AGGGAGCTGG TGTGTGCAGA AGAACTGGGT CACTGGGATG CCTTGGCGGT CATGTCCGGT
    GACGGTCTGA TGCACGAGGT GGTGAATGGG CTAATGGAAC GACCTGACTG GGAGAGTGCC
    ATCCAGAAAC CCCTATGTAG CCTCCCTGGA GGCTCTGGCA ATGCGCTGGC AGCTTCTTTG
    AACTACTATG CTGGGCACGA GCAGGTCACT AATGAAGATC TCCTGATCAA CTGCACACTG
    CTGTTATGCT GCCGGCAGCT GTCACCCATG AATCTGCTGT CCCTGCACAC CGCTTCCGGA
    CGGCAACTCT ATTCTGTTCT CAGTCTGTCC TGGGGTTTCG TTGCTGACGT GGACTTGGAG
    AGTGAGAAAT ACAGGAGCTT GGGGGAGATT CGTTTCACGG TGGGAACCTT TTTTCGCCTA
    GCAAGCCTGC GCATTTACCA AGGCCAACTG GCCTACCTTC CTGTAGGGAA GGCTGCCTCT
    AAGATACCTG CTTCCTCACT GGCACAGAAG GGCCCTGCCA ACACATACCT CGTTCCTCTG
    GAGGAGCCAG TGCCACCTCA TTGGACTGTG GTGCCAGAGC AGGACTTCGT TCTGGTGCTG
    GTGCTGCTTC ACACCCACCT GAGCACAGAG ATGTTTGCAG CACCCATGGG CCGCTGCGAG
    GCTGGTGTTA TGCATCTGTT CTATATACGT GCGGGGGTGT CAAGGGCCAT GCTGCTGCGC
    CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGACCTCG ACTGCCCGTA CTTGGTTCAT
    GTGCCAGTGG TTGCCTTCCG CCTGGAACCC AGGAACCAGA GGGGAGTGTT TTCTGTGGAC
    GGGGAGCTGA TGGTGTGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG
    GTCTCTGGTA GCAGTGACTC CCCGTCCGGC CGGGACTCCC AGCGGAGGCC ACCTCCAGAA
    GAACCAATAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_596877.2
    CDS1728..2879
    Translation

    Target ORF information:

    RefSeq Version NM_133386.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_133386.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCAACCAG CAGACTGTCC CCGGGGACTG CTCCCACGGC CATGCAGAGT GTTGGTGCTG 
    CTGAACCCCC GGGGTGGCAA GGGCAAGGCT CTGAAGCTCT TTCAGAGCCG TGTGCGGCCC
    CTTCTGGAGG AGGCTGAGGT ATCCTTTAAA CTGATGCTCA CCGAACGGCA GAACCATGCC
    AGGGAGCTGG TGTGTGCAGA AGAACTGGGT CACTGGGATG CCTTGGCGGT CATGTCCGGT
    GACGGTCTGA TGCACGAGGT GGTGAATGGG CTAATGGAAC GACCTGACTG GGAGAGTGCC
    ATCCAGAAAC CCCTATGTAG CCTCCCTGGA GGCTCTGGCA ATGCGCTGGC AGCTTCTTTG
    AACTACTATG CTGGGCACGA GCAGGTCACT AATGAAGATC TCCTGATCAA CTGCACACTG
    CTGTTATGCT GCCGGCAGCT GTCACCCATG AATCTGCTGT CCCTGCACAC CGCTTCCGGA
    CGGCAACTCT ATTCTGTTCT CAGTCTGTCC TGGGGTTTCG TTGCTGACGT GGACTTGGAG
    AGTGAGAAAT ACAGGAGCTT GGGGGAGATT CGTTTCACGG TGGGAACCTT TTTTCGCCTA
    GCAAGCCTGC GCATTTACCA AGGCCAACTG GCCTACCTTC CTGTAGGGAA GGCTGCCTCT
    AAGATACCTG CTTCCTCACT GGCACAGAAG GGCCCTGCCA ACACATACCT CGTTCCTCTG
    GAGGAGCCAG TGCCACCTCA TTGGACTGTG GTGCCAGAGC AGGACTTCGT TCTGGTGCTG
    GTGCTGCTTC ACACCCACCT GAGCACAGAG ATGTTTGCAG CACCCATGGG CCGCTGCGAG
    GCTGGTGTTA TGCATCTGTT CTATATACGT GCGGGGGTGT CAAGGGCCAT GCTGCTGCGC
    CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGACCTCG ACTGCCCGTA CTTGGTTCAT
    GTGCCAGTGG TTGCCTTCCG CCTGGAACCC AGGAACCAGA GGGGAGTGTT TTCTGTGGAC
    GGGGAGCTGA TGGTGTGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG
    GTCTCTGGTA GCAGTGACTC CCCGTCCGGC CGGGACTCCC AGCGGAGGCC ACCTCCAGAA
    GAACCAATAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa00646
    Clone ID Related Accession (Same CDS sequence) NM_001270808.1 , NM_133386.3 , NM_001270810.1 , NM_001270809.1 , NM_001270807.1
    Accession Version NM_001270810.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1152bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-10
    Organism Rattus norvegicus(Norway rat)
    Product sphingosine kinase 1 isoform b
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07030835.1 and BC081738.1. Transcript Variant: This variant (5) uses an alternate splice junction at the 3' end of the first exon compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, 4, 5, and 6 all encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB049574.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906347, SAMN01906349 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCAACCAG CAGACTGTCC CCGGGGACTG CTCCCACGGC CATGCAGAGT GTTGGTGCTG 
    CTGAACCCCC GGGGTGGCAA GGGCAAGGCT CTGAAGCTCT TTCAGAGCCG TGTGCGGCCC
    CTTCTGGAGG AGGCTGAGGT ATCCTTTAAA CTGATGCTCA CCGAACGGCA GAACCATGCC
    AGGGAGCTGG TGTGTGCAGA AGAACTGGGT CACTGGGATG CCTTGGCGGT CATGTCCGGT
    GACGGTCTGA TGCACGAGGT GGTGAATGGG CTAATGGAAC GACCTGACTG GGAGAGTGCC
    ATCCAGAAAC CCCTATGTAG CCTCCCTGGA GGCTCTGGCA ATGCGCTGGC AGCTTCTTTG
    AACTACTATG CTGGGCACGA GCAGGTCACT AATGAAGATC TCCTGATCAA CTGCACACTG
    CTGTTATGCT GCCGGCAGCT GTCACCCATG AATCTGCTGT CCCTGCACAC CGCTTCCGGA
    CGGCAACTCT ATTCTGTTCT CAGTCTGTCC TGGGGTTTCG TTGCTGACGT GGACTTGGAG
    AGTGAGAAAT ACAGGAGCTT GGGGGAGATT CGTTTCACGG TGGGAACCTT TTTTCGCCTA
    GCAAGCCTGC GCATTTACCA AGGCCAACTG GCCTACCTTC CTGTAGGGAA GGCTGCCTCT
    AAGATACCTG CTTCCTCACT GGCACAGAAG GGCCCTGCCA ACACATACCT CGTTCCTCTG
    GAGGAGCCAG TGCCACCTCA TTGGACTGTG GTGCCAGAGC AGGACTTCGT TCTGGTGCTG
    GTGCTGCTTC ACACCCACCT GAGCACAGAG ATGTTTGCAG CACCCATGGG CCGCTGCGAG
    GCTGGTGTTA TGCATCTGTT CTATATACGT GCGGGGGTGT CAAGGGCCAT GCTGCTGCGC
    CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGACCTCG ACTGCCCGTA CTTGGTTCAT
    GTGCCAGTGG TTGCCTTCCG CCTGGAACCC AGGAACCAGA GGGGAGTGTT TTCTGTGGAC
    GGGGAGCTGA TGGTGTGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG
    GTCTCTGGTA GCAGTGACTC CCCGTCCGGC CGGGACTCCC AGCGGAGGCC ACCTCCAGAA
    GAACCAATAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001257739.1
    CDS1124..2275
    Translation

    Target ORF information:

    RefSeq Version NM_001270810.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001270810.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCAACCAG CAGACTGTCC CCGGGGACTG CTCCCACGGC CATGCAGAGT GTTGGTGCTG 
    CTGAACCCCC GGGGTGGCAA GGGCAAGGCT CTGAAGCTCT TTCAGAGCCG TGTGCGGCCC
    CTTCTGGAGG AGGCTGAGGT ATCCTTTAAA CTGATGCTCA CCGAACGGCA GAACCATGCC
    AGGGAGCTGG TGTGTGCAGA AGAACTGGGT CACTGGGATG CCTTGGCGGT CATGTCCGGT
    GACGGTCTGA TGCACGAGGT GGTGAATGGG CTAATGGAAC GACCTGACTG GGAGAGTGCC
    ATCCAGAAAC CCCTATGTAG CCTCCCTGGA GGCTCTGGCA ATGCGCTGGC AGCTTCTTTG
    AACTACTATG CTGGGCACGA GCAGGTCACT AATGAAGATC TCCTGATCAA CTGCACACTG
    CTGTTATGCT GCCGGCAGCT GTCACCCATG AATCTGCTGT CCCTGCACAC CGCTTCCGGA
    CGGCAACTCT ATTCTGTTCT CAGTCTGTCC TGGGGTTTCG TTGCTGACGT GGACTTGGAG
    AGTGAGAAAT ACAGGAGCTT GGGGGAGATT CGTTTCACGG TGGGAACCTT TTTTCGCCTA
    GCAAGCCTGC GCATTTACCA AGGCCAACTG GCCTACCTTC CTGTAGGGAA GGCTGCCTCT
    AAGATACCTG CTTCCTCACT GGCACAGAAG GGCCCTGCCA ACACATACCT CGTTCCTCTG
    GAGGAGCCAG TGCCACCTCA TTGGACTGTG GTGCCAGAGC AGGACTTCGT TCTGGTGCTG
    GTGCTGCTTC ACACCCACCT GAGCACAGAG ATGTTTGCAG CACCCATGGG CCGCTGCGAG
    GCTGGTGTTA TGCATCTGTT CTATATACGT GCGGGGGTGT CAAGGGCCAT GCTGCTGCGC
    CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGACCTCG ACTGCCCGTA CTTGGTTCAT
    GTGCCAGTGG TTGCCTTCCG CCTGGAACCC AGGAACCAGA GGGGAGTGTT TTCTGTGGAC
    GGGGAGCTGA TGGTGTGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG
    GTCTCTGGTA GCAGTGACTC CCCGTCCGGC CGGGACTCCC AGCGGAGGCC ACCTCCAGAA
    GAACCAATAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa00646
    Clone ID Related Accession (Same CDS sequence) NM_001270808.1 , NM_133386.3 , NM_001270810.1 , NM_001270809.1 , NM_001270807.1
    Accession Version NM_001270809.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1152bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-10
    Organism Rattus norvegicus(Norway rat)
    Product sphingosine kinase 1 isoform b
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07030835.1 and BC081738.1. Transcript Variant: This variant (4) uses an alternate splice junction at the 3' end of the first exon compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, 4, 5, and 6 all encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB049573.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCAACCAG CAGACTGTCC CCGGGGACTG CTCCCACGGC CATGCAGAGT GTTGGTGCTG 
    CTGAACCCCC GGGGTGGCAA GGGCAAGGCT CTGAAGCTCT TTCAGAGCCG TGTGCGGCCC
    CTTCTGGAGG AGGCTGAGGT ATCCTTTAAA CTGATGCTCA CCGAACGGCA GAACCATGCC
    AGGGAGCTGG TGTGTGCAGA AGAACTGGGT CACTGGGATG CCTTGGCGGT CATGTCCGGT
    GACGGTCTGA TGCACGAGGT GGTGAATGGG CTAATGGAAC GACCTGACTG GGAGAGTGCC
    ATCCAGAAAC CCCTATGTAG CCTCCCTGGA GGCTCTGGCA ATGCGCTGGC AGCTTCTTTG
    AACTACTATG CTGGGCACGA GCAGGTCACT AATGAAGATC TCCTGATCAA CTGCACACTG
    CTGTTATGCT GCCGGCAGCT GTCACCCATG AATCTGCTGT CCCTGCACAC CGCTTCCGGA
    CGGCAACTCT ATTCTGTTCT CAGTCTGTCC TGGGGTTTCG TTGCTGACGT GGACTTGGAG
    AGTGAGAAAT ACAGGAGCTT GGGGGAGATT CGTTTCACGG TGGGAACCTT TTTTCGCCTA
    GCAAGCCTGC GCATTTACCA AGGCCAACTG GCCTACCTTC CTGTAGGGAA GGCTGCCTCT
    AAGATACCTG CTTCCTCACT GGCACAGAAG GGCCCTGCCA ACACATACCT CGTTCCTCTG
    GAGGAGCCAG TGCCACCTCA TTGGACTGTG GTGCCAGAGC AGGACTTCGT TCTGGTGCTG
    GTGCTGCTTC ACACCCACCT GAGCACAGAG ATGTTTGCAG CACCCATGGG CCGCTGCGAG
    GCTGGTGTTA TGCATCTGTT CTATATACGT GCGGGGGTGT CAAGGGCCAT GCTGCTGCGC
    CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGACCTCG ACTGCCCGTA CTTGGTTCAT
    GTGCCAGTGG TTGCCTTCCG CCTGGAACCC AGGAACCAGA GGGGAGTGTT TTCTGTGGAC
    GGGGAGCTGA TGGTGTGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG
    GTCTCTGGTA GCAGTGACTC CCCGTCCGGC CGGGACTCCC AGCGGAGGCC ACCTCCAGAA
    GAACCAATAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001257738.1
    CDS438..1589
    Translation

    Target ORF information:

    RefSeq Version NM_001270809.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001270809.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCAACCAG CAGACTGTCC CCGGGGACTG CTCCCACGGC CATGCAGAGT GTTGGTGCTG 
    CTGAACCCCC GGGGTGGCAA GGGCAAGGCT CTGAAGCTCT TTCAGAGCCG TGTGCGGCCC
    CTTCTGGAGG AGGCTGAGGT ATCCTTTAAA CTGATGCTCA CCGAACGGCA GAACCATGCC
    AGGGAGCTGG TGTGTGCAGA AGAACTGGGT CACTGGGATG CCTTGGCGGT CATGTCCGGT
    GACGGTCTGA TGCACGAGGT GGTGAATGGG CTAATGGAAC GACCTGACTG GGAGAGTGCC
    ATCCAGAAAC CCCTATGTAG CCTCCCTGGA GGCTCTGGCA ATGCGCTGGC AGCTTCTTTG
    AACTACTATG CTGGGCACGA GCAGGTCACT AATGAAGATC TCCTGATCAA CTGCACACTG
    CTGTTATGCT GCCGGCAGCT GTCACCCATG AATCTGCTGT CCCTGCACAC CGCTTCCGGA
    CGGCAACTCT ATTCTGTTCT CAGTCTGTCC TGGGGTTTCG TTGCTGACGT GGACTTGGAG
    AGTGAGAAAT ACAGGAGCTT GGGGGAGATT CGTTTCACGG TGGGAACCTT TTTTCGCCTA
    GCAAGCCTGC GCATTTACCA AGGCCAACTG GCCTACCTTC CTGTAGGGAA GGCTGCCTCT
    AAGATACCTG CTTCCTCACT GGCACAGAAG GGCCCTGCCA ACACATACCT CGTTCCTCTG
    GAGGAGCCAG TGCCACCTCA TTGGACTGTG GTGCCAGAGC AGGACTTCGT TCTGGTGCTG
    GTGCTGCTTC ACACCCACCT GAGCACAGAG ATGTTTGCAG CACCCATGGG CCGCTGCGAG
    GCTGGTGTTA TGCATCTGTT CTATATACGT GCGGGGGTGT CAAGGGCCAT GCTGCTGCGC
    CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGACCTCG ACTGCCCGTA CTTGGTTCAT
    GTGCCAGTGG TTGCCTTCCG CCTGGAACCC AGGAACCAGA GGGGAGTGTT TTCTGTGGAC
    GGGGAGCTGA TGGTGTGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG
    GTCTCTGGTA GCAGTGACTC CCCGTCCGGC CGGGACTCCC AGCGGAGGCC ACCTCCAGAA
    GAACCAATAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa00646
    Clone ID Related Accession (Same CDS sequence) NM_001270808.1 , NM_133386.3 , NM_001270810.1 , NM_001270809.1 , NM_001270807.1
    Accession Version NM_001270807.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1152bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-10
    Organism Rattus norvegicus(Norway rat)
    Product sphingosine kinase 1 isoform b
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07030835.1 and BC081738.1. Transcript Variant: This variant (2) uses an alternate first exon compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, 4, 5, and 6 all encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB049572.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCAACCAG CAGACTGTCC CCGGGGACTG CTCCCACGGC CATGCAGAGT GTTGGTGCTG 
    CTGAACCCCC GGGGTGGCAA GGGCAAGGCT CTGAAGCTCT TTCAGAGCCG TGTGCGGCCC
    CTTCTGGAGG AGGCTGAGGT ATCCTTTAAA CTGATGCTCA CCGAACGGCA GAACCATGCC
    AGGGAGCTGG TGTGTGCAGA AGAACTGGGT CACTGGGATG CCTTGGCGGT CATGTCCGGT
    GACGGTCTGA TGCACGAGGT GGTGAATGGG CTAATGGAAC GACCTGACTG GGAGAGTGCC
    ATCCAGAAAC CCCTATGTAG CCTCCCTGGA GGCTCTGGCA ATGCGCTGGC AGCTTCTTTG
    AACTACTATG CTGGGCACGA GCAGGTCACT AATGAAGATC TCCTGATCAA CTGCACACTG
    CTGTTATGCT GCCGGCAGCT GTCACCCATG AATCTGCTGT CCCTGCACAC CGCTTCCGGA
    CGGCAACTCT ATTCTGTTCT CAGTCTGTCC TGGGGTTTCG TTGCTGACGT GGACTTGGAG
    AGTGAGAAAT ACAGGAGCTT GGGGGAGATT CGTTTCACGG TGGGAACCTT TTTTCGCCTA
    GCAAGCCTGC GCATTTACCA AGGCCAACTG GCCTACCTTC CTGTAGGGAA GGCTGCCTCT
    AAGATACCTG CTTCCTCACT GGCACAGAAG GGCCCTGCCA ACACATACCT CGTTCCTCTG
    GAGGAGCCAG TGCCACCTCA TTGGACTGTG GTGCCAGAGC AGGACTTCGT TCTGGTGCTG
    GTGCTGCTTC ACACCCACCT GAGCACAGAG ATGTTTGCAG CACCCATGGG CCGCTGCGAG
    GCTGGTGTTA TGCATCTGTT CTATATACGT GCGGGGGTGT CAAGGGCCAT GCTGCTGCGC
    CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGACCTCG ACTGCCCGTA CTTGGTTCAT
    GTGCCAGTGG TTGCCTTCCG CCTGGAACCC AGGAACCAGA GGGGAGTGTT TTCTGTGGAC
    GGGGAGCTGA TGGTGTGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG
    GTCTCTGGTA GCAGTGACTC CCCGTCCGGC CGGGACTCCC AGCGGAGGCC ACCTCCAGAA
    GAACCAATAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001257736.1
    CDS488..1639
    Translation

    Target ORF information:

    RefSeq Version NM_001270807.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001270807.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCAACCAG CAGACTGTCC CCGGGGACTG CTCCCACGGC CATGCAGAGT GTTGGTGCTG 
    CTGAACCCCC GGGGTGGCAA GGGCAAGGCT CTGAAGCTCT TTCAGAGCCG TGTGCGGCCC
    CTTCTGGAGG AGGCTGAGGT ATCCTTTAAA CTGATGCTCA CCGAACGGCA GAACCATGCC
    AGGGAGCTGG TGTGTGCAGA AGAACTGGGT CACTGGGATG CCTTGGCGGT CATGTCCGGT
    GACGGTCTGA TGCACGAGGT GGTGAATGGG CTAATGGAAC GACCTGACTG GGAGAGTGCC
    ATCCAGAAAC CCCTATGTAG CCTCCCTGGA GGCTCTGGCA ATGCGCTGGC AGCTTCTTTG
    AACTACTATG CTGGGCACGA GCAGGTCACT AATGAAGATC TCCTGATCAA CTGCACACTG
    CTGTTATGCT GCCGGCAGCT GTCACCCATG AATCTGCTGT CCCTGCACAC CGCTTCCGGA
    CGGCAACTCT ATTCTGTTCT CAGTCTGTCC TGGGGTTTCG TTGCTGACGT GGACTTGGAG
    AGTGAGAAAT ACAGGAGCTT GGGGGAGATT CGTTTCACGG TGGGAACCTT TTTTCGCCTA
    GCAAGCCTGC GCATTTACCA AGGCCAACTG GCCTACCTTC CTGTAGGGAA GGCTGCCTCT
    AAGATACCTG CTTCCTCACT GGCACAGAAG GGCCCTGCCA ACACATACCT CGTTCCTCTG
    GAGGAGCCAG TGCCACCTCA TTGGACTGTG GTGCCAGAGC AGGACTTCGT TCTGGTGCTG
    GTGCTGCTTC ACACCCACCT GAGCACAGAG ATGTTTGCAG CACCCATGGG CCGCTGCGAG
    GCTGGTGTTA TGCATCTGTT CTATATACGT GCGGGGGTGT CAAGGGCCAT GCTGCTGCGC
    CTCTTCCTGG CCATGCAGAA GGGCAAGCAT ATGGACCTCG ACTGCCCGTA CTTGGTTCAT
    GTGCCAGTGG TTGCCTTCCG CCTGGAACCC AGGAACCAGA GGGGAGTGTT TTCTGTGGAC
    GGGGAGCTGA TGGTGTGTGA AGCTGTGCAG GGCCAAGTGC ACCCAAACTA CCTTTGGATG
    GTCTCTGGTA GCAGTGACTC CCCGTCCGGC CGGGACTCCC AGCGGAGGCC ACCTCCAGAA
    GAACCAATAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa09850
    Clone ID Related Accession (Same CDS sequence) NM_001270811.1
    Accession Version NM_001270811.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1377bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-10
    Organism Rattus norvegicus(Norway rat)
    Product sphingosine kinase 1 isoform a
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07030835.1 and BC081738.1. Transcript Variant: This variant (1) encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ486894.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGCAACTGG ACTCTCCACC AACCTCAAAG GATCTGGCCG CCTCCGGGAA TGACGAGGGC 
    GCTCTCACAG CCCAGGGAGG TGGAGAGGGC GAGCCCCACG GCCAGTCACC AGACACCCGC
    CTGGGCAACA CCGATAAGCA GCTGCACACT GGAGCCGCCG TTACCTCTAG CAGCGCCGGG
    GCAGCATCGG TGGCTCCTTG TCAGCGGGAG CCCCGGGACC TGGCTATGCA ACCAGCAGAC
    TGTCCCCGGG GACTGCTCCC ACGGCCATGC AGAGTGTTGG TGCTGCTGAA CCCCCGGGGT
    GGCAAGGGCA AGGCTCTGAA GCTCTTTCAG AGCCGTGTGC GGCCCCTTCT GGAGGAGGCT
    GAGGTATCCT TTAAACTGAT GCTCACCGAA CGGCAGAACC ATGCCAGGGA GCTGGTGTGT
    GCAGAAGAAC TGGGTCACTG GGATGCCTTG GCGGTCATGT CCGGTGACGG TCTGATGCAC
    GAGGTGGTGA ATGGGCTAAT GGAACGACCT GACTGGGAGA GTGCCATCCA GAAACCCCTA
    TGTAGCCTCC CTGGAGGCTC TGGCAATGCG CTGGCAGCTT CTTTGAACTA CTATGCTGGG
    CACGAGCAGG TCACTAATGA AGATCTCCTG ATCAACTGCA CACTGCTGTT ATGCTGCCGG
    CAGCTGTCAC CCATGAATCT GCTGTCCCTG CACACCGCTT CCGGACGGCA ACTCTATTCT
    GTTCTCAGTC TGTCCTGGGG TTTCGTTGCT GACGTGGACT TGGAGAGTGA GAAATACAGG
    AGCTTGGGGG AGATTCGTTT CACGGTGGGA ACCTTTTTTC GCCTAGCAAG CCTGCGCATT
    TACCAAGGCC AACTGGCCTA CCTTCCTGTA GGGAAGGCTG CCTCTAAGAT ACCTGCTTCC
    TCACTGGCAC AGAAGGGCCC TGCCAACACA TACCTCGTTC CTCTGGAGGA GCCAGTGCCA
    CCTCATTGGA CTGTGGTGCC AGAGCAGGAC TTCGTTCTGG TGCTGGTGCT GCTTCACACC
    CACCTGAGCA CAGAGATGTT TGCAGCACCC ATGGGCCGCT GCGAGGCTGG TGTTATGCAT
    CTGTTCTATA TACGTGCGGG GGTGTCAAGG GCCATGCTGC TGCGCCTCTT CCTGGCCATG
    CAGAAGGGCA AGCATATGGA CCTCGACTGC CCGTACTTGG TTCATGTGCC AGTGGTTGCC
    TTCCGCCTGG AACCCAGGAA CCAGAGGGGA GTGTTTTCTG TGGACGGGGA GCTGATGGTG
    TGTGAAGCTG TGCAGGGCCA AGTGCACCCA AACTACCTTT GGATGGTCTC TGGTAGCAGT
    GACTCCCCGT CCGGCCGGGA CTCCCAGCGG AGGCCACCTC CAGAAGAACC AATATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001257740.1
    CDS1249..2625
    Translation

    Target ORF information:

    RefSeq Version NM_001270811.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001270811.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGCAACTGG ACTCTCCACC AACCTCAAAG GATCTGGCCG CCTCCGGGAA TGACGAGGGC 
    GCTCTCACAG CCCAGGGAGG TGGAGAGGGC GAGCCCCACG GCCAGTCACC AGACACCCGC
    CTGGGCAACA CCGATAAGCA GCTGCACACT GGAGCCGCCG TTACCTCTAG CAGCGCCGGG
    GCAGCATCGG TGGCTCCTTG TCAGCGGGAG CCCCGGGACC TGGCTATGCA ACCAGCAGAC
    TGTCCCCGGG GACTGCTCCC ACGGCCATGC AGAGTGTTGG TGCTGCTGAA CCCCCGGGGT
    GGCAAGGGCA AGGCTCTGAA GCTCTTTCAG AGCCGTGTGC GGCCCCTTCT GGAGGAGGCT
    GAGGTATCCT TTAAACTGAT GCTCACCGAA CGGCAGAACC ATGCCAGGGA GCTGGTGTGT
    GCAGAAGAAC TGGGTCACTG GGATGCCTTG GCGGTCATGT CCGGTGACGG TCTGATGCAC
    GAGGTGGTGA ATGGGCTAAT GGAACGACCT GACTGGGAGA GTGCCATCCA GAAACCCCTA
    TGTAGCCTCC CTGGAGGCTC TGGCAATGCG CTGGCAGCTT CTTTGAACTA CTATGCTGGG
    CACGAGCAGG TCACTAATGA AGATCTCCTG ATCAACTGCA CACTGCTGTT ATGCTGCCGG
    CAGCTGTCAC CCATGAATCT GCTGTCCCTG CACACCGCTT CCGGACGGCA ACTCTATTCT
    GTTCTCAGTC TGTCCTGGGG TTTCGTTGCT GACGTGGACT TGGAGAGTGA GAAATACAGG
    AGCTTGGGGG AGATTCGTTT CACGGTGGGA ACCTTTTTTC GCCTAGCAAG CCTGCGCATT
    TACCAAGGCC AACTGGCCTA CCTTCCTGTA GGGAAGGCTG CCTCTAAGAT ACCTGCTTCC
    TCACTGGCAC AGAAGGGCCC TGCCAACACA TACCTCGTTC CTCTGGAGGA GCCAGTGCCA
    CCTCATTGGA CTGTGGTGCC AGAGCAGGAC TTCGTTCTGG TGCTGGTGCT GCTTCACACC
    CACCTGAGCA CAGAGATGTT TGCAGCACCC ATGGGCCGCT GCGAGGCTGG TGTTATGCAT
    CTGTTCTATA TACGTGCGGG GGTGTCAAGG GCCATGCTGC TGCGCCTCTT CCTGGCCATG
    CAGAAGGGCA AGCATATGGA CCTCGACTGC CCGTACTTGG TTCATGTGCC AGTGGTTGCC
    TTCCGCCTGG AACCCAGGAA CCAGAGGGGA GTGTTTTCTG TGGACGGGGA GCTGATGGTG
    TGTGAAGCTG TGCAGGGCCA AGTGCACCCA AACTACCTTT GGATGGTCTC TGGTAGCAGT
    GACTCCCCGT CCGGCCGGGA CTCCCAGCGG AGGCCACCTC CAGAAGAACC AATATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa33963
    Clone ID Related Accession (Same CDS sequence) XM_006247787.2
    Accession Version XM_006247787.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product sphingosine kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006247787.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCAACCAG ACTGTCCCCG GGGACTGCTC CCACGGCCAT GCAGAGTGTT GGTGCTGCTG 
    AACCCCCGGG GTGGCAAGGG CAAGGCTCTG AAGCTCTTTC AGAGCCGTGT GCGGCCCCTT
    CTGGAGGAGG CTGAGGTATC CTTTAAACTG ATGCTCACCG AACGGCAGAA CCATGCCAGG
    GAGCTGGTGT GTGCAGAAGA ACTGGGTCAC TGGGATGCCT TGGCGGTCAT GTCCGGTGAC
    GGTCTGATGC ACGAGGTGGT GAATGGGCTA ATGGAACGAC CTGACTGGGA GAGTGCCATC
    CAGAAACCCC TATGTAGCCT CCCTGGAGGC TCTGGCAATG CGCTGGCAGC TTCTTTGAAC
    TACTATGCTG GGCACGAGCA GGTCACTAAT GAAGATCTCC TGATCAACTG CACACTGCTG
    TTATGCTGCC GGCAGCTGTC ACCCATGAAT CTGCTGTCCC TGCACACCGC TTCCGGACGG
    CAACTCTATT CTGTTCTCAG TCTGTCCTGG GGTTTCGTTG CTGACGTGGA CTTGGAGAGT
    GAGAAATACA GGAGCTTGGG GGAGATTCGT TTCACGGTGG GAACCTTTTT TCGCCTAGCA
    AGCCTGCGCA TTTACCAAGG CCAACTGGCC TACCTTCCTG TAGGGAAGGC TGCCTCTAAG
    ATACCTGCTT CCTCACTGGC ACAGAAGGGC CCTGCCAACA CATACCTCGT TCCTCTGGAG
    GAGCCAGTGC CACCTCATTG GACTGTGGTG CCAGAGCAGG ACTTCGTTCT GGTGCTGGTG
    CTGCTTCACA CCCACCTGAG CACAGAGATG TTTGCAGCAC CCATGGGCCG CTGCGAGGCT
    GGTGTTATGC ATCTGTTCTA TATACGTGCG GGGGTGTCAA GGGCCATGCT GCTGCGCCTC
    TTCCTGGCCA TGCAGAAGGG CAAGCATATG GACCTCGACT GCCCGTACTT GGTTCATGTG
    CCAGTGGTTG CCTTCCGCCT GGAACCCAGG AACCAGAGGG GAGTGTTTTC TGTGGACGGG
    GAGCTGATGG TGTGTGAAGC TGTGCAGGGC CAAGTGCACC CAAACTACCT TTGGATGGTC
    TCTGGTAGCA GTGACTCCCC GTCCGGCCGG GACTCCCAGC GGAGGCCACC TCCAGAAGAA
    CCAATATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006247849.1
    CDS811..1959
    Translation

    Target ORF information:

    RefSeq Version XM_006247787.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus sphingosine kinase 1 (Sphk1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006247787.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCAACCAG ACTGTCCCCG GGGACTGCTC CCACGGCCAT GCAGAGTGTT GGTGCTGCTG 
    AACCCCCGGG GTGGCAAGGG CAAGGCTCTG AAGCTCTTTC AGAGCCGTGT GCGGCCCCTT
    CTGGAGGAGG CTGAGGTATC CTTTAAACTG ATGCTCACCG AACGGCAGAA CCATGCCAGG
    GAGCTGGTGT GTGCAGAAGA ACTGGGTCAC TGGGATGCCT TGGCGGTCAT GTCCGGTGAC
    GGTCTGATGC ACGAGGTGGT GAATGGGCTA ATGGAACGAC CTGACTGGGA GAGTGCCATC
    CAGAAACCCC TATGTAGCCT CCCTGGAGGC TCTGGCAATG CGCTGGCAGC TTCTTTGAAC
    TACTATGCTG GGCACGAGCA GGTCACTAAT GAAGATCTCC TGATCAACTG CACACTGCTG
    TTATGCTGCC GGCAGCTGTC ACCCATGAAT CTGCTGTCCC TGCACACCGC TTCCGGACGG
    CAACTCTATT CTGTTCTCAG TCTGTCCTGG GGTTTCGTTG CTGACGTGGA CTTGGAGAGT
    GAGAAATACA GGAGCTTGGG GGAGATTCGT TTCACGGTGG GAACCTTTTT TCGCCTAGCA
    AGCCTGCGCA TTTACCAAGG CCAACTGGCC TACCTTCCTG TAGGGAAGGC TGCCTCTAAG
    ATACCTGCTT CCTCACTGGC ACAGAAGGGC CCTGCCAACA CATACCTCGT TCCTCTGGAG
    GAGCCAGTGC CACCTCATTG GACTGTGGTG CCAGAGCAGG ACTTCGTTCT GGTGCTGGTG
    CTGCTTCACA CCCACCTGAG CACAGAGATG TTTGCAGCAC CCATGGGCCG CTGCGAGGCT
    GGTGTTATGC ATCTGTTCTA TATACGTGCG GGGGTGTCAA GGGCCATGCT GCTGCGCCTC
    TTCCTGGCCA TGCAGAAGGG CAAGCATATG GACCTCGACT GCCCGTACTT GGTTCATGTG
    CCAGTGGTTG CCTTCCGCCT GGAACCCAGG AACCAGAGGG GAGTGTTTTC TGTGGACGGG
    GAGCTGATGG TGTGTGAAGC TGTGCAGGGC CAAGTGCACC CAAACTACCT TTGGATGGTC
    TCTGGTAGCA GTGACTCCCC GTCCGGCCGG GACTCCCAGC GGAGGCCACC TCCAGAAGAA
    CCAATATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.