Cygb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cygb
Entrez Gene ID	170520
Full Name	cytoglobin
Synonyms	Glnf,Staap,Stap
General protein information	Preferred Names cytoglobin Names cytoglobin HGb globin f histoglobin stellate cell activation-associated protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q32.2
Summary	peroxidase; involved in catabolizing hydrogen peroxide and lipid hydroperoxides in the liver [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_130744.2	NP_570100.1	cytoglobin

Pathways from BioSystems

Homologies

	CYGB	XP_001104888.1
Canis lupus familiaris (dog)	CYGB	NP_001071055.1
Mus musculus (house mouse)	Cygb	NP_084482.1
Danio rerio (zebrafish)	LOC100150453	XP_001923665.2
Xenopus tropicalis (tropical clawed frog)	cygb	NP_001006870.1
Homo sapiens (human)	CYGB	NP_599030.1
Bos taurus (cattle)	CYGB	NP_001193649.1
Gallus gallus (chicken)	CYGB	NP_001008789.1
Rattus norvegicus (Norway rat)	Cygb	NP_570100.1
Pan troglodytes (chimpanzee)	CYGB	XP_003953243.1
Danio rerio (zebrafish)	cygb2	NP_001019395.1

Gene Ontology
Bibliography

Related articles in PubMed

Cytoglobin regulates blood pressure and vascular tone through nitric oxide metabolism in the vascular wall.
Liu X, El-Mahdy MA, Boslett J, Varadharaj S, Hemann C, Abdelghany TM, Ismail RS, Little SC, Zhou D, Thuy LT, Kawada N, Zweier JL
Nature communications814807(2017 Apr)

Intermediate Tyrosyl Radical and Amyloid Structure in Peroxide-Activated Cytoglobin.
Ferreira JC, Marcondes MF, Icimoto MY, Cardoso TH, Tofanello A, Pessoto FS, Miranda EG, Prieto T, Nascimento OR, Oliveira V, Nantes IL
PloS one10(8)e0136554(2015)

Cytoglobin may be involved in the healing process of gastric mucosal injuries in the late phase without angiogenesis.
Tanaka F, Tominaga K, Sasaki E, Sogawa M, Yamagami H, Tanigawa T, Shiba M, Watanabe K, Watanabe T, Fujiwara Y, Kawada N, Yoshizato K, Arakawa T
Digestive diseases and sciences58(5)1198-206(2013 May)

Mechanisms of neuroprotection from hypoxia-ischemia (HI) brain injury by up-regulation of cytoglobin (CYGB) in a neonatal rat model.
Tian SF, Yang HH, Xiao DP, Huang YJ, He GY, Ma HR, Xia F, Shi XC
The Journal of biological chemistry288(22)15988-6003(2013 May)

Cytoglobin, a novel member of the globin family, protects kidney fibroblasts against oxidative stress under ischemic conditions.
Nishi H, Inagi R, Kawada N, Yoshizato K, Mimura I, Fujita T, Nangaku M
The American journal of pathology178(1)128-39(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This result suggests that Cygb possibly participates in the development of degenerative diseases; our findings also support the possible biological role of Cygb related to peroxidase activity.
Title: Intermediate Tyrosyl Radical and Amyloid Structure in Peroxide-Activated Cytoglobin.

may be involved in the healing process of gastric mucosal injuries in the late phase without angiogenesis
Title: Cytoglobin may be involved in the healing process of gastric mucosal injuries in the late phase without angiogenesis.

The reduction of neonatal HI injury by CYGB may be due in part to antioxidant and antiapoptotic mechanisms and by promoting angiogenesis.
Title: Mechanisms of neuroprotection from hypoxia-ischemia (HI) brain injury by up-regulation of cytoglobin (CYGB) in a neonatal rat model.

These findings show for the first that Cygb serves as a defensive mechanism against oxidative stress both in vitro and in vivo.
Title: Cytoglobin, a novel member of the globin family, protects kidney fibroblasts against oxidative stress under ischemic conditions.

The induction of cytoglobin in islets may reduce cell loss from chronic hypoxia and may be a useful adjunct to islet transplantation.
Title: Reduction of ischemic cell death in cultured Islets of Langerhans by the induction of cytoglobin.

The following Cygb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cygb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00441	NM_130744.2 Latest version!	Rattus norvegicus cytoglobin (Cygb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cygb ( NM_130744.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_570100.1 Rattus norvegicus cytoglobin (Cygb), mRNA.

Price & Availability↑

CloneID	ORa00441
Clone ID Related Accession (Same CDS sequence)	NM_130744.2
Accession Version	NM_130744.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	cytoglobin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC088455.1. On Jan 2, 2005 this sequence version replaced NM_130744.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC088455.1, AJ245663.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296 [ECO:0000348] ##Evidence-Data-END##

ATGGAGAAAG TGCCGGGCGA CATGGAGATA GAGCGCAGGG AGAGGAACGA GGAGCTGTCC 
GAGGCGGAGA GGAAGGCGGT TCAGGCTACG TGGGCCCGGC TGTATGCCAA CTGCGAGGAC 
GTGGGGGTGG CCATCCTGGT GAGGTTCTTT GTGAACTTCC CGTCGGCCAA GCAGTACTTC 
AGCCAGTTTA AACACATGGA GGATCCGTTG GAGATGGAGA GGAGTCCTCA GCTGCGGAAA 
CATGCCTGCC GGGTCATGGG GGCCCTCAAC ACGGTCGTGG AGAACCTGCA TGACCCAGAC 
AAGGTATCCT CTGTGCTCGC GCTGGTTGGC AAGGCCCACG CCCTCAAGCA CAAGGTGGAA 
CCTATGTACT TTAAGATTCT CTCTGGGGTC ATTCTGGACG TGATCGCCGA GGAGTTTGCC 
AATGACTTCC CCGTCGAGAC GCAGAAAGCC TGGACCAAGC TGCGCGGTCT CATCTACAGC 
CATGTGACCG CGGCCTACAA GGAAGTGGGC TGGGTACAGC AGGTCCCCAA CACCACCACC 
CTACCAGCCA CACTGCCCTC TTCAGGGCCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_570100.1
CDS	171..743
Translation	MEKVPGDMEIERRERNEELSEAERKAVQATWARLYANCEDVGVAILVRFFVNFPSAKQYFSQFKHMEDPLEMERSPQLRKHACRVMGALNTVVENLHDPDKVSSVLALVGKAHALKHKVEPMYFKILSGVILDVIAEEFANDFPVETQKAWTKLRGLIYSHVTAAYKEVGWVQQVPNTTTLPATLPSSGP

Target ORF information:

RefSeq Version	NM_130744.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytoglobin (Cygb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130744.2

ORF Insert Sequence:

ATGGAGAAAG TGCCGGGCGA CATGGAGATA GAGCGCAGGG AGAGGAACGA GGAGCTGTCC 
GAGGCGGAGA GGAAGGCGGT TCAGGCTACG TGGGCCCGGC TGTATGCCAA CTGCGAGGAC 
GTGGGGGTGG CCATCCTGGT GAGGTTCTTT GTGAACTTCC CGTCGGCCAA GCAGTACTTC 
AGCCAGTTTA AACACATGGA GGATCCGTTG GAGATGGAGA GGAGTCCTCA GCTGCGGAAA 
CATGCCTGCC GGGTCATGGG GGCCCTCAAC ACGGTCGTGG AGAACCTGCA TGACCCAGAC 
AAGGTATCCT CTGTGCTCGC GCTGGTTGGC AAGGCCCACG CCCTCAAGCA CAAGGTGGAA 
CCTATGTACT TTAAGATTCT CTCTGGGGTC ATTCTGGACG TGATCGCCGA GGAGTTTGCC 
AATGACTTCC CCGTCGAGAC GCAGAAAGCC TGGACCAAGC TGCGCGGTCT CATCTACAGC 
CATGTGACCG CGGCCTACAA GGAAGTGGGC TGGGTACAGC AGGTCCCCAA CACCACCACC 
CTACCAGCCA CACTGCCCTC TTCAGGGCCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cytoglobin regulates blood pressure and vascular tone through nitric oxide metabolism in the vascular wall.
Nature communications814807(2017 Apr)
Liu X,El-Mahdy MA,Boslett J,Varadharaj S,Hemann C,Abdelghany TM,Ismail RS,Little SC,Zhou D,Thuy LT,Kawada N,Zweier JL

Intermediate Tyrosyl Radical and Amyloid Structure in Peroxide-Activated Cytoglobin.
PloS one10(8)e0136554(2015)
Ferreira JC,Marcondes MF,Icimoto MY,Cardoso TH,Tofanello A,Pessoto FS,Miranda EG,Prieto T,Nascimento OR,Oliveira V,Nantes IL

Cytoglobin may be involved in the healing process of gastric mucosal injuries in the late phase without angiogenesis.
Digestive diseases and sciences58(5)1198-206(2013 May)
Tanaka F,Tominaga K,Sasaki E,Sogawa M,Yamagami H,Tanigawa T,Shiba M,Watanabe K,Watanabe T,Fujiwara Y,Kawada N,Yoshizato K,Arakawa T

Mechanisms of neuroprotection from hypoxia-ischemia (HI) brain injury by up-regulation of cytoglobin (CYGB) in a neonatal rat model.
The Journal of biological chemistry288(22)15988-6003(2013 May)
Tian SF,Yang HH,Xiao DP,Huang YJ,He GY,Ma HR,Xia F,Shi XC

Cytoglobin, a novel member of the globin family, protects kidney fibroblasts against oxidative stress under ischemic conditions.
The American journal of pathology178(1)128-39(2011 Jan)
Nishi H,Inagi R,Kawada N,Yoshizato K,Mimura I,Fujita T,Nangaku M

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Cygb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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