Abcg8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Abcg8
Entrez Gene ID	155192
Full Name	ATP binding cassette subfamily G member 8
General protein information	Preferred Names ATP binding cassette subfamily G member 8 Names ATP-binding cassette sub-family G member 8 ATP-binding cassette, sub-family G (WHITE), member 8 ATP-binding cassette, subfamily G (WHITE), member 8 sterolin 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q12
Summary	ATP-binding cassette (ABC) transporter that regulates sterol absorption and excretion; mutation causes sitosterolemia [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008764453.2	XP_008762675.1	ATP-binding cassette sub-family G member 8 isoform X1
NM_130414.2	NP_569098.2	ATP-binding cassette sub-family G member 8

Pathways from BioSystems

Homologies

Homo sapiens (human)	ABCG8	NP_071882.1
Rattus norvegicus (Norway rat)	Abcg8	NP_569098.2
Saccharomyces cerevisiae (baker's yeast)	YOL075C	NP_014567.2
Kluyveromyces lactis	KLLA0C04477g	XP_452398.1
Magnaporthe oryzae (rice blast fungus)	MGG_10410	XP_003719428.1
Canis lupus familiaris (dog)	ABCG8	XP_531799.3
Mus musculus (house mouse)	Abcg8	NP_080456.1
Xenopus tropicalis (tropical clawed frog)	abcg8	XP_002932437.2
Arabidopsis thaliana (thale cress)	AT2G01320	NP_178241.1
Pan troglodytes (chimpanzee)	ABCG8	XP_525745.2
Gallus gallus (chicken)	ABCG8	XP_419458.4
Oryza sativa (rice)	Os01g0121700	NP_001041878.1
	ABCG8	XP_001111321.2
Bos taurus (cattle)	ABCG8	NP_001019834.1
Danio rerio (zebrafish)	abcg8	NP_001108041.2

Gene Ontology
Bibliography

Related articles in PubMed

Crystal structure of the human sterol transporter ABCG5/ABCG8.
Lee JY, Kinch LN, Borek DM, Wang J, Wang J, Urbatsch IL, Xie XS, Grishin NV, Cohen JC, Otwinowski Z, Hobbs HH, Rosenbaum DM
Nature533(7604)561-4(2016 05)

Influence of dietary sugar on cholesterol and bile acid metabolism in?the rat: Marked reduction of hepatic Abcg5/8 expression following sucrose ingestion.
Apro J, Beckman L, Angelin B, Rudling M
Biochemical and biophysical research communications461(4)592-7(2015 Jun)

ABCG5/ABCG8-independent biliary cholesterol excretion in lactating rats.
Coy DJ, Wooton-Kee CR, Yan B, Sabeva N, Su K, Graf G, Vore M
American journal of physiology. Gastrointestinal and liver physiology299(1)G228-35(2010 Jul)

Dramatically increased intestinal absorption of cholesterol following hypophysectomy is normalized by thyroid hormone.
G?lman C, Bonde Y, Matasconi M, Angelin B, Rudling M
Gastroenterology134(4)1127-36(2008 Apr)

The different effect of pioglitazone as compared to insulin on expression of hepatic and intestinal genes regulating post-prandial lipoproteins in diabetes.
Lally S, Owens D, Tomkin GH
Atherosclerosis193(2)343-51(2007 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In contrast, the marked reduction of hepatic Abcg5/8 observed is an effect of the high sugar content of the diets.
Title: Influence of dietary sugar on cholesterol and bile acid metabolism in?the rat: Marked reduction of hepatic Abcg5/8 expression following sucrose ingestion.

Data reveal marked utilization of an Abcg5/Abcg8-independent mechanism for basal biliary cholesterol secretion in rats during lactation, but a dependence on Abcg5/g8 for maximal biliary cholesterol secretion.
Title: ABCG5/ABCG8-independent biliary cholesterol excretion in lactating rats.

Hepatic secretion of cholesterol and ABCG5/G8 expression are strongly stimulated in hypophysectomized rats during treatment with thyroid hormone.
Title: Dramatically increased intestinal absorption of cholesterol following hypophysectomy is normalized by thyroid hormone.

Intestinal mRNA expression of Abcg5 (-47%) and Abcg8 (-43%) as well as Abcg5 protein contents are reduced in insulin-deficient animals.
Title: Down-regulation of hepatic and intestinal Abcg5 and Abcg8 expression associated with altered sterol fluxes in rats with streptozotocin-induced diabetes.

The following Abcg8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Abcg8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa44200	XM_008764453.2 Latest version!	Rattus norvegicus ATP binding cassette subfamily G member 8 (Abcg8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa00377	NM_130414.2 Latest version!	Rattus norvegicus ATP binding cassette subfamily G member 8 (Abcg8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Abcg8 ( XM_008764453.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762675.1 Rattus norvegicus ATP binding cassette subfamily G member 8 (Abcg8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa44200
Clone ID Related Accession (Same CDS sequence)	XM_008764453.2
Accession Version	XM_008764453.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ATP-binding cassette sub-family G member 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008764453.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCAG GACAAATCTG GATAAACGGG CAACCCAGCA CGCCTCAGCT GATACAGAAG 
TGTGTGGCAC ATGTGCGCCA GCAAGACCAG CTGCTCCCCA ATCTGACTGT CAGAGAGACC 
CTGACTTTCA TCGCCCAGAT GCGCCTGCCC AAGACCTTCT CTCAGGCCCA GCGAGACAAA 
CGGGTGGAAG ACGTGATTGC GGAGCTGCGG CTGCGGCAGT GCGCCAACAC CCGCGTGGGC 
AACACATACG TACGCGGGGT GTCCGGGGGC GAGCGCCGAA GAGTGAGCAT CGGGGTGCAG 
CTCCTGTGGA ACCCAGGAAT CCTCATCCTG GATGAACCCA CTTCCGGCCT CGACAGCTTC 
ACCGCTCACA ACCTGGTGAG AACTTTGTCC CGCCTGGCCA AAGGCAACAG GCTGGTGCTC 
ATCTCCCTCC ACCAGCCTCG CTCTGACATC TTCAGGCTAT TTGACCTGGT CCTTCTGATG 
ACGTCTGGCA CCCCTATCTA CCTGGGGGTG GCACAGCACA TGGTGCAGTA CTTTACATCA 
ATTGGCTACC CTTGTCCTCG CTACAGCAAC CCTGCTGACT TCTACGTGGA CTTGACGAGC 
ATTGACAGGC GCAGCAAAGA ACAGGAGGTG GCCACCATGG AGAAGGCTCG ATTACTTGCA 
GCCTTGTTCC TAGAAAAAGT GCAAGGCTTT GACGACTTTC TGTGGAAAGC TGAGGCAAAG 
AGTCTCGACA CAGGCACCTA TGCAGTCAGC CAGACCCTCA CACAGGACAC CAACTGTGGA 
ACTGCTGCTG AGCTGCCCGG GATGATACAG CAGTTTACCA CCCTGATCCG TCGTCAGATT 
TCCAATGACT TCCGGGACTT GCCCACCCTG TTCATCCATG GAGCAGAAGC CTGCCTGATG 
TCTCTCATCA TTGGCTTCCT TTACTACGGC CACGCAGACA AGCCGCTCTC CTTCATGGAC 
ATGGCAGCCC TCCTGTTCAT GATAGGAGCA CTCATTCCTT TTAATGTCAT TCTGGATGTC 
GTCTCCAAAT GTCACTCGGA GCGGTCGCTG CTGTACTATG AACTGGAGGA CGGACTGTAC 
ACTGCTGGTC CTTATTTCTT TGCCAAGGTC CTCGGTGAGC TGCCAGAGCA CTGTGCCTAT 
GTCATCATCT ATGGGATGCC CATCTACTGG CTGACCAACC TGCGGCCAGG GCCTGAGCTC 
TTCCTCCTGC ACTTCATGCT TCTGTGGCTG GTGGTGTTCT GCTGCAGGAC CATGGCCCTG 
GCCGCCTCTG CCATGCTGCC CACCTTCCAC ATGTCCTCCT TCTGCTGCAA CGCTCTCTAC 
AACTCCTTCT ACCTTACGGC TGGCTTCATG ATAAACTTGA ACAACCTGTG GATAGTACCT 
GCATGGATTT CCAAGATGTC GTTCCTCCGG TGGTGCTTCT CAGGGCTGAT GCAGATTCAG 
TTTAATGGAC ACATTTACAC CACGCAGATC GGCAACCTCA CCTTCTCCGT CCCCGGAGAC 
GCGATGGTCA CTGCCATGGA CCTGAACTCA CATCCTCTTT ATGCGATCTA CCTCATCGTC 
ATTGGCATCA GCTGTGGCTT CCTGTCCCTG TATTATCTGT CCTTGAAGTT CATCAAACAG 
AAGTCAATTC AAGATTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762675.1
CDS	616..2256
Translation	MKSGQIWINGQPSTPQLIQKCVAHVRQQDQLLPNLTVRETLTFIAQMRLPKTFSQAQRDKRVEDVIAELRLRQCANTRVGNTYVRGVSGGERRRVSIGVQLLWNPGILILDEPTSGLDSFTAHNLVRTLSRLAKGNRLVLISLHQPRSDIFRLFDLVLLMTSGTPIYLGVAQHMVQYFTSIGYPCPRYSNPADFYVDLTSIDRRSKEQEVATMEKARLLAALFLEKVQGFDDFLWKAEAKSLDTGTYAVSQTLTQDTNCGTAAELPGMIQQFTTLIRRQISNDFRDLPTLFIHGAEACLMSLIIGFLYYGHADKPLSFMDMAALLFMIGALIPFNVILDVVSKCHSERSLLYYELEDGLYTAGPYFFAKVLGELPEHCAYVIIYGMPIYWLTNLRPGPELFLLHFMLLWLVVFCCRTMALAASAMLPTFHMSSFCCNALYNSFYLTAGFMINLNNLWIVPAWISKMSFLRWCFSGLMQIQFNGHIYTTQIGNLTFSVPGDAMVTAMDLNSHPLYAIYLIVIGISCGFLSLYYLSLKFIKQKSIQDW

Target ORF information:

RefSeq Version	XM_008764453.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATP binding cassette subfamily G member 8 (Abcg8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764453.2

ORF Insert Sequence:

ATGAAATCAG GACAAATCTG GATAAACGGG CAACCCAGCA CGCCTCAGCT GATACAGAAG 
TGTGTGGCAC ATGTGCGCCA GCAAGACCAG CTGCTCCCCA ATCTGACTGT CAGAGAGACC 
CTGACTTTCA TCGCCCAGAT GCGCCTGCCC AAGACCTTCT CTCAGGCCCA GCGAGACAAA 
CGGGTGGAAG ACGTGATTGC GGAGCTGCGG CTGCGGCAGT GCGCCAACAC CCGCGTGGGC 
AACACATACG TACGCGGGGT GTCCGGGGGC GAGCGCCGAA GAGTGAGCAT CGGGGTGCAG 
CTCCTGTGGA ACCCAGGAAT CCTCATCCTG GATGAACCCA CTTCCGGCCT CGACAGCTTC 
ACCGCTCACA ACCTGGTGAG AACTTTGTCC CGCCTGGCCA AAGGCAACAG GCTGGTGCTC 
ATCTCCCTCC ACCAGCCTCG CTCTGACATC TTCAGGCTAT TTGACCTGGT CCTTCTGATG 
ACGTCTGGCA CCCCTATCTA CCTGGGGGTG GCACAGCACA TGGTGCAGTA CTTTACATCA 
ATTGGCTACC CTTGTCCTCG CTACAGCAAC CCTGCTGACT TCTACGTGGA CTTGACGAGC 
ATTGACAGGC GCAGCAAAGA ACAGGAGGTG GCCACCATGG AGAAGGCTCG ATTACTTGCA 
GCCTTGTTCC TAGAAAAAGT GCAAGGCTTT GACGACTTTC TGTGGAAAGC TGAGGCAAAG 
AGTCTCGACA CAGGCACCTA TGCAGTCAGC CAGACCCTCA CACAGGACAC CAACTGTGGA 
ACTGCTGCTG AGCTGCCCGG GATGATACAG CAGTTTACCA CCCTGATCCG TCGTCAGATT 
TCCAATGACT TCCGGGACTT GCCCACCCTG TTCATCCATG GAGCAGAAGC CTGCCTGATG 
TCTCTCATCA TTGGCTTCCT TTACTACGGC CACGCAGACA AGCCGCTCTC CTTCATGGAC 
ATGGCAGCCC TCCTGTTCAT GATAGGAGCA CTCATTCCTT TTAATGTCAT TCTGGATGTC 
GTCTCCAAAT GTCACTCGGA GCGGTCGCTG CTGTACTATG AACTGGAGGA CGGACTGTAC 
ACTGCTGGTC CTTATTTCTT TGCCAAGGTC CTCGGTGAGC TGCCAGAGCA CTGTGCCTAT 
GTCATCATCT ATGGGATGCC CATCTACTGG CTGACCAACC TGCGGCCAGG GCCTGAGCTC 
TTCCTCCTGC ACTTCATGCT TCTGTGGCTG GTGGTGTTCT GCTGCAGGAC CATGGCCCTG 
GCCGCCTCTG CCATGCTGCC CACCTTCCAC ATGTCCTCCT TCTGCTGCAA CGCTCTCTAC 
AACTCCTTCT ACCTTACGGC TGGCTTCATG ATAAACTTGA ACAACCTGTG GATAGTACCT 
GCATGGATTT CCAAGATGTC GTTCCTCCGG TGGTGCTTCT CAGGGCTGAT GCAGATTCAG 
TTTAATGGAC ACATTTACAC CACGCAGATC GGCAACCTCA CCTTCTCCGT CCCCGGAGAC 
GCGATGGTCA CTGCCATGGA CCTGAACTCA CATCCTCTTT ATGCGATCTA CCTCATCGTC 
ATTGGCATCA GCTGTGGCTT CCTGTCCCTG TATTATCTGT CCTTGAAGTT CATCAAACAG 
AAGTCAATTC AAGATTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Abcg8 ( NM_130414.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_569098.2 Rattus norvegicus ATP binding cassette subfamily G member 8 (Abcg8), mRNA.

Price & Availability↑

CloneID	ORa00377
Clone ID Related Accession (Same CDS sequence)	NM_130414.2
Accession Version	NM_130414.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Rattus norvegicus(Norway rat)
Product	ATP-binding cassette sub-family G member 8
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF351785.2. On or before Dec 17, 2003 this sequence version replaced XM_346758.1, NM_130414.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF351785.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689598, SAMN01906355 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGAGA AGACCAAAGA GGAGACCCAG CTGTGGAACG GGACTGTACT CCAGGATGCT 
TCAAGCCTCC AGGACAGCGT GTTCTCCTCT GAAAGTGACA ACAGCCTCTA CTTCACCTAC 
AGTGGTCAGT CCAACACTCT GGAGGTCAGA GATCTCACCT ACCAGGTGGA CATGGCCTCT 
CAGGTGCCTT GGTTTGAGCA GCTGGCTCAG TTCAAGTTAC CGTGGAGGTC TCGCGGCAGC 
CAGGACTCCT GGGATCTGGG CATCCGAAAT CTGAGCTTCA AAGTGAGGAG TGGACAGATG 
CTGGCTATCA TAGGGAGCGC AGGCTGCGGG AGAGCCACAT TACTCGACGT TATCACAGGC 
AGAGACCATG GTGGCAAGAT GAAATCAGGA CAAATCTGGA TAAACGGGCA ACCCAGCACG 
CCTCAGCTGA TACAGAAGTG TGTGGCACAT GTGCGCCAGC AAGACCAGCT GCTCCCCAAT 
CTGACTGTCA GAGAGACCCT GACTTTCATC GCCCAGATGC GCCTGCCCAA GACCTTCTCT 
CAGGCCCAGC GAGACAAACG GGTGGAAGAC GTGATTGCGG AGCTGCGGCT GCGGCAGTGC 
GCCAACACCC GCGTGGGCAA CACATACGTA CGCGGGGTGT CCGGGGGCGA GCGCCGAAGA 
GTGAGCATCG GGGTGCAGCT CCTGTGGAAC CCAGGAATCC TCATCCTGGA TGAACCCACT 
TCCGGCCTCG ACAGCTTCAC CGCTCACAAC CTGGTGAGAA CTTTGTCCCG CCTGGCCAAA 
GGCAACAGGC TGGTGCTCAT CTCCCTCCAC CAGCCTCGCT CTGACATCTT CAGGCTATTT 
GACCTGGTCC TTCTGATGAC GTCTGGCACC CCTATCTACC TGGGGGTGGC ACAGCACATG 
GTGCAGTACT TTACATCAAT TGGCTACCCT TGTCCTCGCT ACAGCAACCC TGCTGACTTC 
TACGTGGACT TGACGAGCAT TGACAGGCGC AGCAAAGAAC AGGAGGTGGC CACCATGGAG 
AAGGCTCGAT TACTTGCAGC CTTGTTCCTA GAAAAAGTGC AAGGCTTTGA CGACTTTCTG 
TGGAAAGCTG AGGCAAAGAG TCTCGACACA GGCACCTATG CAGTCAGCCA GACCCTCACA 
CAGGACACCA ACTGTGGAAC TGCTGCTGAG CTGCCCGGGA TGATACAGCA GTTTACCACC 
CTGATCCGTC GTCAGATTTC CAATGACTTC CGGGACTTGC CCACCCTGTT CATCCATGGA 
GCAGAAGCCT GCCTGATGTC TCTCATCATT GGCTTCCTTT ACTACGGCCA CGCAGACAAG 
CCGCTCTCCT TCATGGACAT GGCAGCCCTC CTGTTCATGA TAGGAGCACT CATTCCTTTT 
AATGTCATTC TGGATGTCGT CTCCAAATGT CACTCGGAGC GGTCGCTGCT GTACTATGAA 
CTGGAGGACG GACTGTACAC TGCTGGTCCT TATTTCTTTG CCAAGGTCCT CGGTGAGCTG 
CCAGAGCACT GTGCCTATGT CATCATCTAT GGGATGCCCA TCTACTGGCT GACCAACCTG 
CGGCCAGGGC CTGAGCTCTT CCTCCTGCAC TTCATGCTTC TGTGGCTGGT GGTGTTCTGC 
TGCAGGACCA TGGCCCTGGC CGCCTCTGCC ATGCTGCCCA CCTTCCACAT GTCCTCCTTC 
TGCTGCAACG CTCTCTACAA CTCCTTCTAC CTTACGGCTG GCTTCATGAT AAACTTGAAC 
AACCTGTGGA TAGTACCTGC ATGGATTTCC AAGATGTCGT TCCTCCGGTG GTGCTTCTCA 
GGGCTGATGC AGATTCAGTT TAATGGACAC ATTTACACCA CGCAGATCGG CAACCTCACC 
TTCTCCGTCC CCGGAGACGC GATGGTCACT GCCATGGACC TGAACTCACA TCCTCTTTAT 
GCGATCTACC TCATCGTCAT TGGCATCAGC TGTGGCTTCC TGTCCCTGTA TTATCTGTCC 
TTGAAGTTCA TCAAACAGAA GTCAATTCAA GATTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569098.2
CDS	111..2129
Translation	MAEKTKEETQLWNGTVLQDASSLQDSVFSSESDNSLYFTYSGQSNTLEVRDLTYQVDMASQVPWFEQLAQFKLPWRSRGSQDSWDLGIRNLSFKVRSGQMLAIIGSAGCGRATLLDVITGRDHGGKMKSGQIWINGQPSTPQLIQKCVAHVRQQDQLLPNLTVRETLTFIAQMRLPKTFSQAQRDKRVEDVIAELRLRQCANTRVGNTYVRGVSGGERRRVSIGVQLLWNPGILILDEPTSGLDSFTAHNLVRTLSRLAKGNRLVLISLHQPRSDIFRLFDLVLLMTSGTPIYLGVAQHMVQYFTSIGYPCPRYSNPADFYVDLTSIDRRSKEQEVATMEKARLLAALFLEKVQGFDDFLWKAEAKSLDTGTYAVSQTLTQDTNCGTAAELPGMIQQFTTLIRRQISNDFRDLPTLFIHGAEACLMSLIIGFLYYGHADKPLSFMDMAALLFMIGALIPFNVILDVVSKCHSERSLLYYELEDGLYTAGPYFFAKVLGELPEHCAYVIIYGMPIYWLTNLRPGPELFLLHFMLLWLVVFCCRTMALAASAMLPTFHMSSFCCNALYNSFYLTAGFMINLNNLWIVPAWISKMSFLRWCFSGLMQIQFNGHIYTTQIGNLTFSVPGDAMVTAMDLNSHPLYAIYLIVIGISCGFLSLYYLSLKFIKQKSIQDW

Target ORF information:

RefSeq Version	NM_130414.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATP binding cassette subfamily G member 8 (Abcg8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130414.2

ORF Insert Sequence:

ATGGCTGAGA AGACCAAAGA GGAGACCCAG CTGTGGAACG GGACTGTACT CCAGGATGCT 
TCAAGCCTCC AGGACAGCGT GTTCTCCTCT GAAAGTGACA ACAGCCTCTA CTTCACCTAC 
AGTGGTCAGT CCAACACTCT GGAGGTCAGA GATCTCACCT ACCAGGTGGA CATGGCCTCT 
CAGGTGCCTT GGTTTGAGCA GCTGGCTCAG TTCAAGTTAC CGTGGAGGTC TCGCGGCAGC 
CAGGACTCCT GGGATCTGGG CATCCGAAAT CTGAGCTTCA AAGTGAGGAG TGGACAGATG 
CTGGCTATCA TAGGGAGCGC AGGCTGCGGG AGAGCCACAT TACTCGACGT TATCACAGGC 
AGAGACCATG GTGGCAAGAT GAAATCAGGA CAAATCTGGA TAAACGGGCA ACCCAGCACG 
CCTCAGCTGA TACAGAAGTG TGTGGCACAT GTGCGCCAGC AAGACCAGCT GCTCCCCAAT 
CTGACTGTCA GAGAGACCCT GACTTTCATC GCCCAGATGC GCCTGCCCAA GACCTTCTCT 
CAGGCCCAGC GAGACAAACG GGTGGAAGAC GTGATTGCGG AGCTGCGGCT GCGGCAGTGC 
GCCAACACCC GCGTGGGCAA CACATACGTA CGCGGGGTGT CCGGGGGCGA GCGCCGAAGA 
GTGAGCATCG GGGTGCAGCT CCTGTGGAAC CCAGGAATCC TCATCCTGGA TGAACCCACT 
TCCGGCCTCG ACAGCTTCAC CGCTCACAAC CTGGTGAGAA CTTTGTCCCG CCTGGCCAAA 
GGCAACAGGC TGGTGCTCAT CTCCCTCCAC CAGCCTCGCT CTGACATCTT CAGGCTATTT 
GACCTGGTCC TTCTGATGAC GTCTGGCACC CCTATCTACC TGGGGGTGGC ACAGCACATG 
GTGCAGTACT TTACATCAAT TGGCTACCCT TGTCCTCGCT ACAGCAACCC TGCTGACTTC 
TACGTGGACT TGACGAGCAT TGACAGGCGC AGCAAAGAAC AGGAGGTGGC CACCATGGAG 
AAGGCTCGAT TACTTGCAGC CTTGTTCCTA GAAAAAGTGC AAGGCTTTGA CGACTTTCTG 
TGGAAAGCTG AGGCAAAGAG TCTCGACACA GGCACCTATG CAGTCAGCCA GACCCTCACA 
CAGGACACCA ACTGTGGAAC TGCTGCTGAG CTGCCCGGGA TGATACAGCA GTTTACCACC 
CTGATCCGTC GTCAGATTTC CAATGACTTC CGGGACTTGC CCACCCTGTT CATCCATGGA 
GCAGAAGCCT GCCTGATGTC TCTCATCATT GGCTTCCTTT ACTACGGCCA CGCAGACAAG 
CCGCTCTCCT TCATGGACAT GGCAGCCCTC CTGTTCATGA TAGGAGCACT CATTCCTTTT 
AATGTCATTC TGGATGTCGT CTCCAAATGT CACTCGGAGC GGTCGCTGCT GTACTATGAA 
CTGGAGGACG GACTGTACAC TGCTGGTCCT TATTTCTTTG CCAAGGTCCT CGGTGAGCTG 
CCAGAGCACT GTGCCTATGT CATCATCTAT GGGATGCCCA TCTACTGGCT GACCAACCTG 
CGGCCAGGGC CTGAGCTCTT CCTCCTGCAC TTCATGCTTC TGTGGCTGGT GGTGTTCTGC 
TGCAGGACCA TGGCCCTGGC CGCCTCTGCC ATGCTGCCCA CCTTCCACAT GTCCTCCTTC 
TGCTGCAACG CTCTCTACAA CTCCTTCTAC CTTACGGCTG GCTTCATGAT AAACTTGAAC 
AACCTGTGGA TAGTACCTGC ATGGATTTCC AAGATGTCGT TCCTCCGGTG GTGCTTCTCA 
GGGCTGATGC AGATTCAGTT TAATGGACAC ATTTACACCA CGCAGATCGG CAACCTCACC 
TTCTCCGTCC CCGGAGACGC GATGGTCACT GCCATGGACC TGAACTCACA TCCTCTTTAT 
GCGATCTACC TCATCGTCAT TGGCATCAGC TGTGGCTTCC TGTCCCTGTA TTATCTGTCC 
TTGAAGTTCA TCAAACAGAA GTCAATTCAA GATTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Crystal structure of the human sterol transporter ABCG5/ABCG8.
Nature533(7604)561-4(2016 05)
Lee JY,Kinch LN,Borek DM,Wang J,Wang J,Urbatsch IL,Xie XS,Grishin NV,Cohen JC,Otwinowski Z,Hobbs HH,Rosenbaum DM

Influence of dietary sugar on cholesterol and bile acid metabolism in?the rat: Marked reduction of hepatic Abcg5/8 expression following sucrose ingestion.
Biochemical and biophysical research communications461(4)592-7(2015 Jun)
Apro J,Beckman L,Angelin B,Rudling M

ABCG5/ABCG8-independent biliary cholesterol excretion in lactating rats.
American journal of physiology. Gastrointestinal and liver physiology299(1)G228-35(2010 Jul)
Coy DJ,Wooton-Kee CR,Yan B,Sabeva N,Su K,Graf G,Vore M

Dramatically increased intestinal absorption of cholesterol following hypophysectomy is normalized by thyroid hormone.
Gastroenterology134(4)1127-36(2008 Apr)
G?lman C,Bonde Y,Matasconi M,Angelin B,Rudling M

The different effect of pioglitazone as compared to insulin on expression of hepatic and intestinal genes regulating post-prandial lipoproteins in diabetes.
Atherosclerosis193(2)343-51(2007 Aug)
Lally S,Owens D,Tomkin GH