Trim63 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trim63
Entrez Gene ID	140939
Full Name	tripartite motif containing 63
Synonyms	Murf,Murf1,Rnf28
General protein information	Preferred Names tripartite motif containing 63 Names E3 ubiquitin-protein ligase TRIM63 MURF-1 RING-type E3 ubiquitin transferase TRIM63 muscle ring finger protein 1 muscle-specific RING finger protein 1 ring finger protein 28 tripartite motif containing 63, E3 ubiquitin protein ligase tripartite motif protein 63 tripartite motif-containing protein 63
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q36
Summary	ubiquitin ligase that is upregulated in models of muscle atrophy; may facilitate protein degradation and skeletal muscle atrophy [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_080903.1	NP_543179.1	E3 ubiquitin-protein ligase TRIM63

Pathways from BioSystems

Homologies

	TRIM63	XP_001108306.1
Bos taurus (cattle)	TRIM63	NP_001039760.1
Mus musculus (house mouse)	Trim63	NP_001034137.2
Rattus norvegicus (Norway rat)	Trim63	NP_543179.1
Canis lupus familiaris (dog)	TRIM63	XP_005617821.1
Xenopus tropicalis (tropical clawed frog)	trim63	NP_001072557.1
Homo sapiens (human)	TRIM63	NP_115977.2
Pan troglodytes (chimpanzee)	TRIM63	XP_524625.1
Gallus gallus (chicken)	TRIM63	XP_424369.4

Gene Ontology
Bibliography

Related articles in PubMed

Forkhead box O3 plays a role in skeletal muscle atrophy through expression of E3 ubiquitin ligases MuRF-1 and atrogin-1 in Cushing's syndrome.
Kang SH, Lee HA, Kim M, Lee E, Sohn UD, Kim I
American journal of physiology. Endocrinology and metabolism312(6)E495-E507(2017 Jun)

Dysregulation between TRIM63/FBXO32 expression and soleus muscle wasting in diabetic rats: potential role of miR-1-3p, -29a/b-3p, and -133a/b-3p.
Gerlinger-Romero F, Yonamine CY, Junior DC, Esteves JV, Machado UF
Molecular and cellular biochemistry427(1-2)187-199(2017 Mar)

UBE2D2 is not involved in MuRF1-dependent muscle wasting during hindlimb suspension.
Polge C, Koulmann N, Claustre A, Jarzaguet M, Serrurier B, Combaret L, B?chet D, Bigard X, Attaix D, Taillandier D
The international journal of biochemistry & cell biology79488-493(2016 10)

Investigation of the Expression of Myogenic Transcription Factors, microRNAs and Muscle-Specific E3 Ubiquitin Ligases in the Medial Gastrocnemius and Soleus Muscles following Peripheral Nerve Injury.
Wiberg R, Jonsson S, Novikova LN, Kingham PJ
PloS one10(12)e0142699(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that FOXO3a plays a role in muscle atrophy through regulation of expression of MuRF1 and atrogin-1 in skeletal muscle in Cushing's syndrome. (FOXO3a = forkhead box protein O3a; MuRF1 = muscle-specific RING finger protein 1)
Title: Forkhead box O3 plays a role in skeletal muscle atrophy through expression of E3 ubiquitin ligases MuRF-1 and atrogin-1 in Cushing's syndrome.

Independent of the metabolic condition, after muscle contraction, both TRIM63 and FBXO32 proteins correlated significantly with miR-1-3p, miR-29a/b-3p, and miR-133a/b-3p.
Title: Dysregulation between TRIM63/FBXO32 expression and soleus muscle wasting in diabetic rats: potential role of miR-1-3p, -29a/b-3p, and -133a/b-3p.

UBE2D2 is not the cognate ubiquitinating enzyme for MuRF1
Title: UBE2D2 is not involved in MuRF1-dependent muscle wasting during hindlimb suspension.

MuRF1 and MuRF2 translocate to the nuclei in the soleus muscle during gravitational unloading.
Title: [Translocation of MuRF isoforms to nuclei in postural muscle during gravitational unloading].

The muscles also showed significantly contrasting transcriptional regulation of the microRNAs miR-1 and miR-206. MuRF1 and Atrogin-1 showed the highest levels of expression in the denervated gastrocnemius muscle
Title: Investigation of the Expression of Myogenic Transcription Factors, microRNAs and Muscle-Specific E3 Ubiquitin Ligases in the Medial Gastrocnemius and Soleus Muscles following Peripheral Nerve Injury.

The following Trim63 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trim63 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12509	NM_080903.1 Latest version!	Rattus norvegicus tripartite motif containing 63 (Trim63), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trim63 ( NM_080903.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_543179.1 Rattus norvegicus tripartite motif containing 63 (Trim63), mRNA.

Price & Availability↑

CloneID	ORa12509
Clone ID Related Accession (Same CDS sequence)	NM_080903.1
Accession Version	NM_080903.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase TRIM63
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY059627.1. On Jun 26, 2012 this sequence version replaced XM_003750058.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY059627.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGATTATA AATCTGGCTT GATTCCGGAC GGAAATGCTA TGGAGAACCT GGAGAAGCAG 
CTCATCTGCC CCATCTGCCT TGAGATGTTT ACCAAGCCTG TGGTCATCCT GCCCTGCCAG 
CACAACCTCT GCCGGAAGTG TGCCAACGAC ATCTTCCAGG CTGCCAATCC CTACTGGACC 
AACCGCGGTG GCTCGGTGTC CATGTCTGGA GGTCGTTTCC GCTGCCCCTC GTGCCGCCAT 
GAAGTGATCA TGGACCGGCA TGGGGTGTAC GGTCTGCAGA GGAACCTGCT GGTGGAGAAC 
ATCATCGACA TCTACAAGCA GGAATGCTCC AGTCGGCCCC TGCAGAAAGG CAGCCACCCG 
ATGTGCAAGG AACACGAAGA CGAGAAAATC AACATCTACT GTCTCACGTG CGAGGTGCCT 
ACTTGCTCCT TGTGCAAGGT GTTCGGGGCT CACCAGGCCT GTGAAGTTGC CCCCTTACAA 
AGCATCTTCC AAGGACAGAA GACTGAACTG AGCAATTGCA TCTCCATGCT GGTGGCAGGG 
AACGACCGAG TTCAGACTAT CATCTCGCAG CTGGAGGACT CCTGCCGAGT GACCAAGGAA 
AACAGCCACC AGGTGAAGGA GGAACTGAGC CACAAGTTTG ACGCCCTCTA CGCCATCCTG 
GACGAGAAGA AGAGTGAGCT GCTGCAGCGG ATCACTCAGG AGCAGGAGGA GAAGCTGGAC 
TTCATCGAGG CCCTGATCCT CCAGTACCGA GAGCAGTTGG AAAAGTCGAC CAAGCTTGTG 
GAAACAGCCA TCCAGTCCCT GGATGAGCCC GGAGGGGCCA CCTTCCTCTT GAGTGCCAAG 
CCGCTCATCA AGAGCATTGT AGAAGCTTCC AAGGGCTGCC AGCTGGGGAA GACAGAACAA 
GGCTTTGAGA ACATGGACTA CTTTACTCTG AATTTAGAAC ACATAGCAGA GGCCTTGAGG 
GCCATCGACT TTGGGACAGA TGAGGAGGAG GAGTTTACTG AAGAGGAGGA GGAGGAGGAT 
CAAGAAGAGG GCGTGTCCAC AGAGGGACAC CAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_543179.1
CDS	95..1150
Translation	MDYKSGLIPDGNAMENLEKQLICPICLEMFTKPVVILPCQHNLCRKCANDIFQAANPYWTNRGGSVSMSGGRFRCPSCRHEVIMDRHGVYGLQRNLLVENIIDIYKQECSSRPLQKGSHPMCKEHEDEKINIYCLTCEVPTCSLCKVFGAHQACEVAPLQSIFQGQKTELSNCISMLVAGNDRVQTIISQLEDSCRVTKENSHQVKEELSHKFDALYAILDEKKSELLQRITQEQEEKLDFIEALILQYREQLEKSTKLVETAIQSLDEPGGATFLLSAKPLIKSIVEASKGCQLGKTEQGFENMDYFTLNLEHIAEALRAIDFGTDEEEEFTEEEEEEDQEEGVSTEGHQ

Target ORF information:

RefSeq Version	NM_080903.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tripartite motif containing 63 (Trim63), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080903.1

ORF Insert Sequence:

ATGGATTATA AATCTGGCTT GATTCCGGAC GGAAATGCTA TGGAGAACCT GGAGAAGCAG 
CTCATCTGCC CCATCTGCCT TGAGATGTTT ACCAAGCCTG TGGTCATCCT GCCCTGCCAG 
CACAACCTCT GCCGGAAGTG TGCCAACGAC ATCTTCCAGG CTGCCAATCC CTACTGGACC 
AACCGCGGTG GCTCGGTGTC CATGTCTGGA GGTCGTTTCC GCTGCCCCTC GTGCCGCCAT 
GAAGTGATCA TGGACCGGCA TGGGGTGTAC GGTCTGCAGA GGAACCTGCT GGTGGAGAAC 
ATCATCGACA TCTACAAGCA GGAATGCTCC AGTCGGCCCC TGCAGAAAGG CAGCCACCCG 
ATGTGCAAGG AACACGAAGA CGAGAAAATC AACATCTACT GTCTCACGTG CGAGGTGCCT 
ACTTGCTCCT TGTGCAAGGT GTTCGGGGCT CACCAGGCCT GTGAAGTTGC CCCCTTACAA 
AGCATCTTCC AAGGACAGAA GACTGAACTG AGCAATTGCA TCTCCATGCT GGTGGCAGGG 
AACGACCGAG TTCAGACTAT CATCTCGCAG CTGGAGGACT CCTGCCGAGT GACCAAGGAA 
AACAGCCACC AGGTGAAGGA GGAACTGAGC CACAAGTTTG ACGCCCTCTA CGCCATCCTG 
GACGAGAAGA AGAGTGAGCT GCTGCAGCGG ATCACTCAGG AGCAGGAGGA GAAGCTGGAC 
TTCATCGAGG CCCTGATCCT CCAGTACCGA GAGCAGTTGG AAAAGTCGAC CAAGCTTGTG 
GAAACAGCCA TCCAGTCCCT GGATGAGCCC GGAGGGGCCA CCTTCCTCTT GAGTGCCAAG 
CCGCTCATCA AGAGCATTGT AGAAGCTTCC AAGGGCTGCC AGCTGGGGAA GACAGAACAA 
GGCTTTGAGA ACATGGACTA CTTTACTCTG AATTTAGAAC ACATAGCAGA GGCCTTGAGG 
GCCATCGACT TTGGGACAGA TGAGGAGGAG GAGTTTACTG AAGAGGAGGA GGAGGAGGAT 
CAAGAAGAGG GCGTGTCCAC AGAGGGACAC CAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Forkhead box O3 plays a role in skeletal muscle atrophy through expression of E3 ubiquitin ligases MuRF-1 and atrogin-1 in Cushing's syndrome.
American journal of physiology. Endocrinology and metabolism312(6)E495-E507(2017 Jun)
Kang SH,Lee HA,Kim M,Lee E,Sohn UD,Kim I

Dysregulation between TRIM63/FBXO32 expression and soleus muscle wasting in diabetic rats: potential role of miR-1-3p, -29a/b-3p, and -133a/b-3p.
Molecular and cellular biochemistry427(1-2)187-199(2017 Mar)
Gerlinger-Romero F,Yonamine CY,Junior DC,Esteves JV,Machado UF

UBE2D2 is not involved in MuRF1-dependent muscle wasting during hindlimb suspension.
The international journal of biochemistry & cell biology79488-493(2016 10)
Polge C,Koulmann N,Claustre A,Jarzaguet M,Serrurier B,Combaret L,B?chet D,Bigard X,Attaix D,Taillandier D

Investigation of the Expression of Myogenic Transcription Factors, microRNAs and Muscle-Specific E3 Ubiquitin Ligases in the Medial Gastrocnemius and Soleus Muscles following Peripheral Nerve Injury.
PloS one10(12)e0142699(2015)
Wiberg R,Jonsson S,Novikova LN,Kingham PJ