Tnfsf11 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tnfsf11
Entrez Gene ID	117516
Full Name	TNF superfamily member 11
Synonyms	RANKL
General protein information	Preferred Names TNF superfamily member 11 Names tumor necrosis factor ligand superfamily member 11 ODF OPGL TNF-related activation-induced cytokine TRANCE osteoclast differentiation factor osteoprotegerin ligand receptor activator of nuclear factor kappa-B ligand tumor necrosis factor (ligand) superfamily, member 11 tumor necrosis factor superfamily member 11
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15q11
Summary	induces osteoclast formation and bone resoprtion; plays a role in regulation of calcium homeostasis and osteoclast function [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_057149.1	NP_476490.1	tumor necrosis factor ligand superfamily member 11

Pathways from BioSystems

Homologies

Bos taurus (cattle)	TNFSF11	NP_001192699.1
Pan troglodytes (chimpanzee)	TNFSF11	XP_522750.3
Gallus gallus (chicken)	TNFSF11	NP_001076830.1
Homo sapiens (human)	TNFSF11	NP_003692.1
Mus musculus (house mouse)	Tnfsf11	NP_035743.2
Rattus norvegicus (Norway rat)	Tnfsf11	XP_006252436.1
	TNFSF11	XP_001092669.1
Canis lupus familiaris (dog)	TNFSF11	XP_851765.1
Xenopus tropicalis (tropical clawed frog)	tnfsf11	XP_002938089.1

Gene Ontology
Bibliography

Related articles in PubMed

Interleukin 17 regulates SHP-2 and IL-17RA/STAT-3 dependent Cyr61, IL-23 and GM-CSF expression and RANKL mediated osteoclastogenesis by fibroblast-like synoviocytes in rheumatoid arthritis.
Ganesan R, Rasool M
Molecular immunology91134-144(2017 11)

Evolution of Periodontal Disease: Immune Response and RANK/RANKL/OPG System.
Gibertoni F, Sommer MEL, Esquisatto MAM, Amaral MECD, Oliveira CA, Andrade TAM, Mendon?a FAS, Santamaria-Jr M, Felonato M
Brazilian dental journal28(6)679-687(2017 Nov-Dec)

RANK/RANKL/OPG Expression in Rapid Maxillary Expansion.
Arnez MFM, Ribeiro LSN, Barretto GD, Monteiro PM, Ervolino E, Stuani MBS
Brazilian dental journal28(3)296-300(2017 May-Jun)

Tumor necrosis factor-? antagonist diminishes osteocytic RANKL and sclerostin expression in diabetes rats with periodontitis.
Kim JH, Kim AR, Choi YH, Jang S, Woo GH, Cha JH, Bak EJ, Yoo YJ
PloS one12(12)e0189702(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings demonstrate that IL-17 regulates SHP-2 expression and IL-17RA/STAT-3 dependent production of Cyr61, IL-23, GM-CSF and RANKL in AA-FLS and may reveal a new insight into the pathogenesis of RA.
Title: Interleukin 17 regulates SHP-2 and IL-17RA/STAT-3 dependent Cyr61, IL-23 and GM-CSF expression and RANKL mediated osteoclastogenesis by fibroblast-like synoviocytes in rheumatoid arthritis.

FGF21 inhibited VSMCs calcification via OPG/RANKL system, and through P38 andPI3K/AKT pathways.
Title: Fibroblast growth factor 21 plays an inhibitory role in vascular calcification in?vitro through OPG/RANKL system.

this study shows that flavonoids of Astragalus exert protective effects against adjuvant arthritis by regulating OPG/RANKL/NF-kappaB pathway
Title: Protective effects of total flavonoids of Astragalus against adjuvant-induced arthritis in rats by regulating OPG/RANKL/NF-?B pathway.

TNF-alpha might mediate alveolar bone loss via inducing expression of osteocytic RANKL and sclerostin in type 1 diabetes rats with periodontitis
Title: Tumor necrosis factor-? antagonist diminishes osteocytic RANKL and sclerostin expression in diabetes rats with periodontitis.

the canonical Wnt pathway inhibitor DKK1 blocked the osteogenesis effect and rescued the ratio of RANKL/OPG in periodontal ligament stem cells under force treatment for 1h
Title: Mechanical stress regulates osteogenic differentiation and RANKL/OPG ratio in periodontal ligament stem cells by the Wnt/?-catenin pathway.

The following Tnfsf11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tnfsf11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13390	NM_057149.1 Latest version!	Rattus norvegicus TNF superfamily member 11 (Tnfsf11), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tnfsf11 ( NM_057149.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_476490.1 Rattus norvegicus TNF superfamily member 11 (Tnfsf11), mRNA.

Price & Availability↑

CloneID	ORa13390
Clone ID Related Accession (Same CDS sequence)	NM_057149.1
Accession Version	NM_057149.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Rattus norvegicus(Norway rat)
Product	tumor necrosis factor ligand superfamily member 11
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF187319.1. On or before Dec 15, 2013 this sequence version replaced XM_006252374.1, XM_003751521.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF187319.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689601, SAMN02642659 [ECO:0000348] ##Evidence-Data-END##

ATGCGCCGGG CCAACCGAGA CTACGGCAAG TACCTGCGCG GCTCGGAGGA GATGGGCAGT 
TGCCCTGGCG TCCCACACGA GGGTCCGCTG CATCCCGCGC CTTCAGCACC GGCTCCAGCG 
CCGCCCCCCG CCGCCTCCCG CTTCATGTTC CTGGCGCTCC TGGGGCTGGG ACTGGGTCAG 
GTGGTCTGCA GCATCGCTCT GTTCCTGTAC TTTCGAGCGC AGATGGATCC TAACAGAATA 
TCAGAAGACA GCACGCGCTG CTTCTACAGA ATTCTGAGAC TCCGTGAAAA TACAGGTTTG 
CAGGACTCGA CTCTGGAGAG CGAAGACACA GAAGCACTAC CTGACTCATG CAGGAGAATG 
AAACAAGCCT TTCAAGGGGC CGTGCAAAGG GAATTACAAC ACATTGTGGG GCCACAGCGC 
TTCTCAGGAG TTCCAGCTAT GATGGAAGGT TCGTGGCTCG ATGTGGCCCG GCGGGGCAAG 
CCTGAGGCTC AGCCGTTTGC TCACCTCACC ATCAATGCTG CCGACATCCC ATCGGGTTCC 
CATAAAGTCA GTCTGTCCTC TTGGTACCAT GATCGAGGCT GGGCCAAGAT CTCTAACATG 
ACGTTAAGCA ACGGAAAACT AAGGGTTAAC CAAGATGGCT TCTATTACCT GTACGCCAAC 
ATTTGCTTCA GGCATCATGA AACCTCAGGG AGCGTACCTG CGGACTATCT TCAGCTGATG 
GTATATGTCG TTAAAACCAG CATCAAAATC CCAAGTTCGC ATAACCTGAT GAAAGGGGGG 
AGCACTAAGA ACTGGTCAGG GAATTCTGAA TTCCACTTTT ATTCCATAAA CGTTGGAGGA 
TTTTTCAAGC TCCGGGCTGG TGAGGAAATT AGCGTCCAGG TGTCCAACCC TTCCCTGTTG 
GATCCGGATC AAGATGCGAC GTACTTTGGG GCTTTCAAAG TTCAAGACAT AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476490.1
CDS	1..957
Translation	MRRANRDYGKYLRGSEEMGSCPGVPHEGPLHPAPSAPAPAPPPAASRFMFLALLGLGLGQVVCSIALFLYFRAQMDPNRISEDSTRCFYRILRLRENTGLQDSTLESEDTEALPDSCRRMKQAFQGAVQRELQHIVGPQRFSGVPAMMEGSWLDVARRGKPEAQPFAHLTINAADIPSGSHKVSLSSWYHDRGWAKISNMTLSNGKLRVNQDGFYYLYANICFRHHETSGSVPADYLQLMVYVVKTSIKIPSSHNLMKGGSTKNWSGNSEFHFYSINVGGFFKLRAGEEISVQVSNPSLLDPDQDATYFGAFKVQDID

Target ORF information:

RefSeq Version	NM_057149.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus TNF superfamily member 11 (Tnfsf11), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057149.1

ORF Insert Sequence:

ATGCGCCGGG CCAACCGAGA CTACGGCAAG TACCTGCGCG GCTCGGAGGA GATGGGCAGT 
TGCCCTGGCG TCCCACACGA GGGTCCGCTG CATCCCGCGC CTTCAGCACC GGCTCCAGCG 
CCGCCCCCCG CCGCCTCCCG CTTCATGTTC CTGGCGCTCC TGGGGCTGGG ACTGGGTCAG 
GTGGTCTGCA GCATCGCTCT GTTCCTGTAC TTTCGAGCGC AGATGGATCC TAACAGAATA 
TCAGAAGACA GCACGCGCTG CTTCTACAGA ATTCTGAGAC TCCGTGAAAA TACAGGTTTG 
CAGGACTCGA CTCTGGAGAG CGAAGACACA GAAGCACTAC CTGACTCATG CAGGAGAATG 
AAACAAGCCT TTCAAGGGGC CGTGCAAAGG GAATTACAAC ACATTGTGGG GCCACAGCGC 
TTCTCAGGAG TTCCAGCTAT GATGGAAGGT TCGTGGCTCG ATGTGGCCCG GCGGGGCAAG 
CCTGAGGCTC AGCCGTTTGC TCACCTCACC ATCAATGCTG CCGACATCCC ATCGGGTTCC 
CATAAAGTCA GTCTGTCCTC TTGGTACCAT GATCGAGGCT GGGCCAAGAT CTCTAACATG 
ACGTTAAGCA ACGGAAAACT AAGGGTTAAC CAAGATGGCT TCTATTACCT GTACGCCAAC 
ATTTGCTTCA GGCATCATGA AACCTCAGGG AGCGTACCTG CGGACTATCT TCAGCTGATG 
GTATATGTCG TTAAAACCAG CATCAAAATC CCAAGTTCGC ATAACCTGAT GAAAGGGGGG 
AGCACTAAGA ACTGGTCAGG GAATTCTGAA TTCCACTTTT ATTCCATAAA CGTTGGAGGA 
TTTTTCAAGC TCCGGGCTGG TGAGGAAATT AGCGTCCAGG TGTCCAACCC TTCCCTGTTG 
GATCCGGATC AAGATGCGAC GTACTTTGGG GCTTTCAAAG TTCAAGACAT AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Interleukin 17 regulates SHP-2 and IL-17RA/STAT-3 dependent Cyr61, IL-23 and GM-CSF expression and RANKL mediated osteoclastogenesis by fibroblast-like synoviocytes in rheumatoid arthritis.
Molecular immunology91134-144(2017 11)
Ganesan R,Rasool M

Evolution of Periodontal Disease: Immune Response and RANK/RANKL/OPG System.
Brazilian dental journal28(6)679-687(2017 Nov-Dec)
Gibertoni F,Sommer MEL,Esquisatto MAM,Amaral MECD,Oliveira CA,Andrade TAM,Mendon?a FAS,Santamaria-Jr M,Felonato M

RANK/RANKL/OPG Expression in Rapid Maxillary Expansion.
Brazilian dental journal28(3)296-300(2017 May-Jun)
Arnez MFM,Ribeiro LSN,Barretto GD,Monteiro PM,Ervolino E,Stuani MBS

Tumor necrosis factor-? antagonist diminishes osteocytic RANKL and sclerostin expression in diabetes rats with periodontitis.
PloS one12(12)e0189702(2017)
Kim JH,Kim AR,Choi YH,Jang S,Woo GH,Cha JH,Bak EJ,Yoo YJ