Pfkfb3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pfkfb3
Entrez Gene ID	117276
Full Name	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
General protein information	Preferred Names 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 Names 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 6PF-2-K/Fru-2,6-P2ASE brain-type isozyme 6PF-2-K/Fru-2,6-P2ase 3 PFK/FBPase 3 RB2K
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 17 Map Location: 17q12.2
Summary	catalyzes the biosynthesis and degradation of fructose 2,6-bisphosphate, which plays a role in glycolysis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006254197.3	XP_006254259.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform X5
XM_006254198.3	XP_006254260.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform X6
XM_006254192.3	XP_006254254.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform X1
XM_006254193.3	XP_006254255.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform X2
XM_006254196.3	XP_006254258.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform X4
XM_017600444.1	XP_017455933.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform X3
NM_057135.1	NP_476476.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	Y110A7A.6	NP_491529.2
Canis lupus familiaris (dog)	PFKFB3	XP_005617225.1
Mus musculus (house mouse)	Pfkfb3	NP_001171228.1
Pan troglodytes (chimpanzee)	PFKFB3	XP_001147864.2
Rattus norvegicus (Norway rat)	Pfkfb3	NP_476476.1
Danio rerio (zebrafish)	pfkfb3	NP_998562.1
Homo sapiens (human)	PFKFB3	NP_004557.1
Bos taurus (cattle)	PFKFB3	NP_001071305.1
Xenopus tropicalis (tropical clawed frog)	pfkfb3	NP_001135656.1
	PFKFB3	XP_001107535.1
Gallus gallus (chicken)	PFKFB3	XP_416472.2
Caenorhabditis elegans (roundworm)	K02B2.1	NP_500893.2

Gene Ontology
Bibliography

Related articles in PubMed

Role of Akt/PKB and PFKFB isoenzymes in the control of glycolysis, cell proliferation and protein synthesis in mitogen-stimulated thymocytes.
Houddane A, Bultot L, Novellasdemunt L, Johanns M, Gueuning MA, Vertommen D, Coulie PG, Bartrons R, Hue L, Rider MH
Cellular signalling3423-37(2017 Jun)

PFKFB3-mediated glycolysis is involved in reactive astrocyte proliferation after oxygen-glucose deprivation/reperfusion and is regulated by Cdh1.
Lv Y, Zhang B, Zhai C, Qiu J, Zhang Y, Yao W, Zhang C
Neurochemistry international9126-33(2015 Dec)

Moderate grade hyperammonemia activates lactate dehydrogenase-4 and 6-phosphofructo-2-kinase to support increased lactate turnover in the brain slices.
Mehrotra A, Trigun SK
Molecular and cellular biochemistry381(1-2)157-61(2013 Sep)

Excitotoxic stimulus stabilizes PFKFB3 causing pentose-phosphate pathway to glycolysis switch and neurodegeneration.
Rodriguez-Rodriguez P, Fernandez E, Almeida A, Bola?os JP
Cell death and differentiation19(10)1582-9(2012 Oct)

Switches in 6-phosphofructo-2-kinase isoenzyme expression during rat sperm maturation.
G?mez M, Navarro-Sabat? A, Manzano A, Duran J, Obach M, Bartrons R
Biochemical and biophysical research communications387(2)330-5(2009 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Increased PFKFB3 expression, but not increased PFKFB3 Ser461 phosphorylation, plays a role in increasing glycolysis in mitogen-stimulated thymocytes.
Title: Role of Akt/PKB and PFKFB isoenzymes in the control of glycolysis, cell proliferation and protein synthesis in mitogen-stimulated thymocytes.

astrocyte proliferation can be effectively suppressed by down-regulation of PFKFB3 through control of glycolytic flux, which is downstream of Cdh1
Title: PFKFB3-mediated glycolysis is involved in reactive astrocyte proliferation after oxygen-glucose deprivation/reperfusion and is regulated by Cdh1.

The PFK2 expression along with LDH-4 were observed to be increased ~2-fold (P < 0.001) in 0.5 mM ammonia treated brain slices.
Title: Moderate grade hyperammonemia activates lactate dehydrogenase-4 and 6-phosphofructo-2-kinase to support increased lactate turnover in the brain slices.

The expression of PFKFB3 lacking the APC(Cdh1)-targeting KEN motif, promoted accumulation of PFKFB3 and its release from the nucleus to the cytosol through an excess Cdh1-inhibitable process.
Title: Excitotoxic stimulus stabilizes PFKFB3 causing pentose-phosphate pathway to glycolysis switch and neurodegeneration.

The maximum expression of Pfkfb3 was detected before puberty, while that of Pfkfb4 was observed in adulthood during rat sperm maturation.
Title: Switches in 6-phosphofructo-2-kinase isoenzyme expression during rat sperm maturation.

The following Pfkfb3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pfkfb3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa11431	NM_057135.1 Latest version!	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa38016	XM_006254192.3 Latest version!	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa38017	XM_006254193.3 Latest version!	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa38019	XM_006254196.3 Latest version!	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa38020	XM_006254197.3 Latest version!	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa38021	XM_006254198.3 Latest version!	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa58989	XM_017600444.1 Latest version!	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pfkfb3 ( NM_057135.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_476476.1 Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3), mRNA.

Price & Availability↑

CloneID	ORa11431
Clone ID Related Accession (Same CDS sequence)	NM_057135.1
Accession Version	NM_057135.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D87240.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D87240.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCCTTTGG AACTGACCCA GAGCCGGGTG CAGAAGATCT GGGTGCCTGT CGATCACCGA 
CCCTCGTTGC CCAGATCCTG TGGGCCGAAG CTGACCAACT CCCCAACGGT GATTGTGATG 
GTGGGGCTCC CAGCCCGGGG TAAGACTTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC 
TGGATAGGTG TTCCAACGAA AGTGTTCAAT GTGGGAGAAT ACCGACGGGA GGCTGTGAAG 
CAATACAGCT CTTACAACTT CTTCCGCCCT GACAATGAGG AGGCCATGAG AGTCCGAAAA 
CAGTGTGCCT TAGCTGCCCT GAGAGACGTC AAAAGCTACC TGACAAAGGA AGGAGGACAG 
ATTGCAGTTT TTGATGCCAC CAATACGACG AGAGAAAGGA GGCACATGAT CCTTCATTTT 
GCCAAAGAAA ATGACTTCAA GGCATTTTTC ATCGAGTCCG TCTGTGACGA CCCTACAGTT 
GTGGCCTCCA ATATCATGGA AGTGAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCC 
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAACTGCT ACGAAGCCAG CTACCAGCCT 
CTTGACCCTG ATAAATGTGA CAGGGACCTG TCATTCATCA AAGTGATTGA CGTTGGCCGG 
AGGTTCTTGG TGAACAGAGT GCAGGACCAC ATCCAGAGCC GAATCGTGTA TTACCTGATG 
AACATCCACG TGCAGCCCCG GACCATCTAC CTGTGTCGGC ACGGCGAGAA TGAGTACAAC 
GTGCAGGGCA AGATTGGGGG CGACTCAGGC CTGTCCAGCA GAGGCAAGAA GTTCGCCAAT 
GCCCTGAGCA AGTTCGTTGA AGAGCAGAAT CTGAAGGACC TCCGCGTGTG GACCAGTCAG 
CTGAAAAGTA CCATCCAGAC CGCGGAGGCC CTAAGGCTCC CCTACGAGCA GTGGAAGGCG 
CTCAATGAGA TTGATGCTGG TGTGTGTGAG GAACTAACCT ATGAGGAGAT CAGAGACACC 
TACCCTGAGG AGTATGCACT GCGTGAGCAA GACAAATACT ACTACCGCTA TCCCACAGGC 
GAGTCCTACC AGGATCTTGT CCAGCGCCTG GAGCCTGTGA TCATGGAATT AGAGCGCCAA 
GAGAACGTAT TGGTCATCTG TCACCAGGCT GTCCTGCGCT GCCTACTAGC CTACTTCCTT 
GACAAGAGTG CAGAGGAGAT GCCATACCTG AAATGCCCAC TTCACACAGT CCTGAAACTG 
ACGCCCGTGG CGTATGGGTG CCGTGTGGAG TCCATTTACT TGAATGTAGA ATCAGTGAGC 
ACGCATCGGG AGAGGTCAGA GGCTGTCAAA ATACAACACT TTGCCAGTGT AGTCAGACCT 
TCCTCTTACA CCGAACTTGA CTTTCTGTCT GTGGAGTCCG CAAAACAGGA TGCAAAGAAG 
GGACCTAACC CTCTCATGAG ACGCAATAGT GTCACCCCAC TAGCCAGCCC CGAGCCCACC 
AAAAAGCCTC GCATCAACAG CTTTGAGGAG CATGTGGCTT CCACTTCTGC TGCCCTGCCA 
AGCTGCCTGC CCCCGGAAGT GCCCACGCAG CTGCCCGGAC AACCTTTGCT AGGGAAAGCC 
TGTCTACGAA CTGTCTGTCA CATTTTTTCA AAGTTTTCTC CTTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476476.1
CDS	406..2073
Translation	MPLELTQSRVQKIWVPVDHRPSLPRSCGPKLTNSPTVIVMVGLPARGKTYISKKLTRYLNWIGVPTKVFNVGEYRREAVKQYSSYNFFRPDNEEAMRVRKQCALAALRDVKSYLTKEGGQIAVFDATNTTRERRHMILHFAKENDFKAFFIESVCDDPTVVASNIMEVKISSPDYKDCNSAEAMDDFMKRINCYEASYQPLDPDKCDRDLSFIKVIDVGRRFLVNRVQDHIQSRIVYYLMNIHVQPRTIYLCRHGENEYNVQGKIGGDSGLSSRGKKFANALSKFVEEQNLKDLRVWTSQLKSTIQTAEALRLPYEQWKALNEIDAGVCEELTYEEIRDTYPEEYALREQDKYYYRYPTGESYQDLVQRLEPVIMELERQENVLVICHQAVLRCLLAYFLDKSAEEMPYLKCPLHTVLKLTPVAYGCRVESIYLNVESVSTHRERSEAVKIQHFASVVRPSSYTELDFLSVESAKQDAKKGPNPLMRRNSVTPLASPEPTKKPRINSFEEHVASTSAALPSCLPPEVPTQLPGQPLLGKACLRTVCHIFSKFSPY

Target ORF information:

RefSeq Version	NM_057135.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057135.1

ORF Insert Sequence:

ATGCCTTTGG AACTGACCCA GAGCCGGGTG CAGAAGATCT GGGTGCCTGT CGATCACCGA 
CCCTCGTTGC CCAGATCCTG TGGGCCGAAG CTGACCAACT CCCCAACGGT GATTGTGATG 
GTGGGGCTCC CAGCCCGGGG TAAGACTTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC 
TGGATAGGTG TTCCAACGAA AGTGTTCAAT GTGGGAGAAT ACCGACGGGA GGCTGTGAAG 
CAATACAGCT CTTACAACTT CTTCCGCCCT GACAATGAGG AGGCCATGAG AGTCCGAAAA 
CAGTGTGCCT TAGCTGCCCT GAGAGACGTC AAAAGCTACC TGACAAAGGA AGGAGGACAG 
ATTGCAGTTT TTGATGCCAC CAATACGACG AGAGAAAGGA GGCACATGAT CCTTCATTTT 
GCCAAAGAAA ATGACTTCAA GGCATTTTTC ATCGAGTCCG TCTGTGACGA CCCTACAGTT 
GTGGCCTCCA ATATCATGGA AGTGAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCC 
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAACTGCT ACGAAGCCAG CTACCAGCCT 
CTTGACCCTG ATAAATGTGA CAGGGACCTG TCATTCATCA AAGTGATTGA CGTTGGCCGG 
AGGTTCTTGG TGAACAGAGT GCAGGACCAC ATCCAGAGCC GAATCGTGTA TTACCTGATG 
AACATCCACG TGCAGCCCCG GACCATCTAC CTGTGTCGGC ACGGCGAGAA TGAGTACAAC 
GTGCAGGGCA AGATTGGGGG CGACTCAGGC CTGTCCAGCA GAGGCAAGAA GTTCGCCAAT 
GCCCTGAGCA AGTTCGTTGA AGAGCAGAAT CTGAAGGACC TCCGCGTGTG GACCAGTCAG 
CTGAAAAGTA CCATCCAGAC CGCGGAGGCC CTAAGGCTCC CCTACGAGCA GTGGAAGGCG 
CTCAATGAGA TTGATGCTGG TGTGTGTGAG GAACTAACCT ATGAGGAGAT CAGAGACACC 
TACCCTGAGG AGTATGCACT GCGTGAGCAA GACAAATACT ACTACCGCTA TCCCACAGGC 
GAGTCCTACC AGGATCTTGT CCAGCGCCTG GAGCCTGTGA TCATGGAATT AGAGCGCCAA 
GAGAACGTAT TGGTCATCTG TCACCAGGCT GTCCTGCGCT GCCTACTAGC CTACTTCCTT 
GACAAGAGTG CAGAGGAGAT GCCATACCTG AAATGCCCAC TTCACACAGT CCTGAAACTG 
ACGCCCGTGG CGTATGGGTG CCGTGTGGAG TCCATTTACT TGAATGTAGA ATCAGTGAGC 
ACGCATCGGG AGAGGTCAGA GGCTGTCAAA ATACAACACT TTGCCAGTGT AGTCAGACCT 
TCCTCTTACA CCGAACTTGA CTTTCTGTCT GTGGAGTCCG CAAAACAGGA TGCAAAGAAG 
GGACCTAACC CTCTCATGAG ACGCAATAGT GTCACCCCAC TAGCCAGCCC CGAGCCCACC 
AAAAAGCCTC GCATCAACAG CTTTGAGGAG CATGTGGCTT CCACTTCTGC TGCCCTGCCA 
AGCTGCCTGC CCCCGGAAGT GCCCACGCAG CTGCCCGGAC AACCTTTGCT AGGGAAAGCC 
TGTCTACGAA CTGTCTGTCA CATTTTTTCA AAGTTTTCTC CTTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pfkfb3 ( XM_006254192.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006254254.1 Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa38016
Clone ID Related Accession (Same CDS sequence)	XM_006254192.3
Accession Version	XM_006254192.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006254192.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTGG AACTGACCCA GAGCCGGGTG CAGAAGATCT GGGTGCCTGT CGATCACCGA 
CCCTCGTTGC CCAGATCCTG TGGGCCGAAG CTGACCAACT CCCCAACGGT GATTGTGATG 
GTGGGGCTCC CAGCCCGGGG TAAGACTTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC 
TGGATAGGTG TTCCAACGAA AGTGTTCAAT GTGGGAGAAT ACCGACGGGA GGCTGTGAAG 
CAATACAGCT CTTACAACTT CTTCCGCCCT GACAATGAGG AGGCCATGAG AGTCCGAAAA 
CAGTGTGCCT TAGCTGCCCT GAGAGACGTC AAAAGCTACC TGACAAAGGA AGGAGGACAG 
ATTGCAGTTT TTGATGCCAC CAATACAACG AGAGAAAGGA GGCACATGAT CCTTCATTTT 
GCCAAAGAAA ATGACTTCAA GGCATTTTTC ATCGAGTCCG TCTGTGACGA CCCTACAGTT 
GTGGCCTCCA ATATCATGGA AGTGAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCC 
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAACTGCT ACGAAGCCAG CTACCAGCCT 
CTTGACCCTG ATAAATGTGA CAGGGACCTG TCATTCATCA AAGTGATTGA CGTTGGCCGG 
AGGTTCTTGG TGAACAGAGT GCAGGACCAC ATCCAGAGCC GAATCGTGTA TTACCTGATG 
AACATCCACG TGCAGCCCCG GACCATCTAC CTGTGTCGGC ACGGCGAGAA TGAGTACAAC 
GTGCAGGGCA AGATTGGGGG CGACTCAGGC CTGTCCAGCA GAGGCAAGAA GTTCGCCAAT 
GCCCTGAGCA AGTTCGTTGA AGAGCAGAAT CTGAAGGACC TCCGCGTGTG GACCAGTCAG 
CTGAAAAGTA CCATCCAGAC CGCGGAGGCC CTAAGGCTCC CCTACGAGCA GTGGAAGGCG 
CTCAATGAGA TTGATGCTGG TGTGTGTGAG GAACTAACCT ATGAGGAGAT CAGAGACACC 
TACCCTGAGG AGTATGCACT GCGTGAGCAA GACAAATACT ACTACCGCTA TCCCACAGGC 
GAGTCCTACC AGGATCTTGT CCAGCGCCTG GAGCCTGTGA TCATGGAATT AGAGCGCCAA 
GAGAACGTAT TGGTCATCTG TCACCAGGCT GTCCTGCGCT GCCTACTAGC CTACTTCCTT 
GACAAGAGTG CAGAGGAGAT GCCATACCTG AAATGCCCAC TTCACACAGT CCTGAAACTG 
ACGCCCGTGG CGTATGGGTG CCGTGTGGAG TCCATTTACT TGAATGTAGA ATCAGTGAGC 
ACGCATCGGG AGAGGTCAGA GGCTGTCAAA ATACAACACT TTGCCAGTGT AGTCAGACCT 
TCCTCTTACA CCGAACTTGA CTTTCTGTCT GTGGAGTCCG CAAAACAGGA TGCAAAGAAG 
GGACCTAACC CTCTCATGAG ACGCAATAGT GTCACCCCAC TAGCCAGCCC CGAGCCCACC 
AAAAAGCCTC GCATCAACAG CTTTGAGGAG CATGTGGCTT CCACTTCTGC TGCCCTGCCA 
AGCTGCCTGC CCCCGGAAGT GCCCACGCAG CTGCCCGGAC AAAACATGAG GAGCCCCCGG 
AGCGGTGCCG AATCTTCCCA GAAGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006254254.1
CDS	401..2050
Translation	MPLELTQSRVQKIWVPVDHRPSLPRSCGPKLTNSPTVIVMVGLPARGKTYISKKLTRYLNWIGVPTKVFNVGEYRREAVKQYSSYNFFRPDNEEAMRVRKQCALAALRDVKSYLTKEGGQIAVFDATNTTRERRHMILHFAKENDFKAFFIESVCDDPTVVASNIMEVKISSPDYKDCNSAEAMDDFMKRINCYEASYQPLDPDKCDRDLSFIKVIDVGRRFLVNRVQDHIQSRIVYYLMNIHVQPRTIYLCRHGENEYNVQGKIGGDSGLSSRGKKFANALSKFVEEQNLKDLRVWTSQLKSTIQTAEALRLPYEQWKALNEIDAGVCEELTYEEIRDTYPEEYALREQDKYYYRYPTGESYQDLVQRLEPVIMELERQENVLVICHQAVLRCLLAYFLDKSAEEMPYLKCPLHTVLKLTPVAYGCRVESIYLNVESVSTHRERSEAVKIQHFASVVRPSSYTELDFLSVESAKQDAKKGPNPLMRRNSVTPLASPEPTKKPRINSFEEHVASTSAALPSCLPPEVPTQLPGQNMRSPRSGAESSQKH

Target ORF information:

RefSeq Version	XM_006254192.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006254192.3

ORF Insert Sequence:

ATGCCTTTGG AACTGACCCA GAGCCGGGTG CAGAAGATCT GGGTGCCTGT CGATCACCGA 
CCCTCGTTGC CCAGATCCTG TGGGCCGAAG CTGACCAACT CCCCAACGGT GATTGTGATG 
GTGGGGCTCC CAGCCCGGGG TAAGACTTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC 
TGGATAGGTG TTCCAACGAA AGTGTTCAAT GTGGGAGAAT ACCGACGGGA GGCTGTGAAG 
CAATACAGCT CTTACAACTT CTTCCGCCCT GACAATGAGG AGGCCATGAG AGTCCGAAAA 
CAGTGTGCCT TAGCTGCCCT GAGAGACGTC AAAAGCTACC TGACAAAGGA AGGAGGACAG 
ATTGCAGTTT TTGATGCCAC CAATACAACG AGAGAAAGGA GGCACATGAT CCTTCATTTT 
GCCAAAGAAA ATGACTTCAA GGCATTTTTC ATCGAGTCCG TCTGTGACGA CCCTACAGTT 
GTGGCCTCCA ATATCATGGA AGTGAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCC 
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAACTGCT ACGAAGCCAG CTACCAGCCT 
CTTGACCCTG ATAAATGTGA CAGGGACCTG TCATTCATCA AAGTGATTGA CGTTGGCCGG 
AGGTTCTTGG TGAACAGAGT GCAGGACCAC ATCCAGAGCC GAATCGTGTA TTACCTGATG 
AACATCCACG TGCAGCCCCG GACCATCTAC CTGTGTCGGC ACGGCGAGAA TGAGTACAAC 
GTGCAGGGCA AGATTGGGGG CGACTCAGGC CTGTCCAGCA GAGGCAAGAA GTTCGCCAAT 
GCCCTGAGCA AGTTCGTTGA AGAGCAGAAT CTGAAGGACC TCCGCGTGTG GACCAGTCAG 
CTGAAAAGTA CCATCCAGAC CGCGGAGGCC CTAAGGCTCC CCTACGAGCA GTGGAAGGCG 
CTCAATGAGA TTGATGCTGG TGTGTGTGAG GAACTAACCT ATGAGGAGAT CAGAGACACC 
TACCCTGAGG AGTATGCACT GCGTGAGCAA GACAAATACT ACTACCGCTA TCCCACAGGC 
GAGTCCTACC AGGATCTTGT CCAGCGCCTG GAGCCTGTGA TCATGGAATT AGAGCGCCAA 
GAGAACGTAT TGGTCATCTG TCACCAGGCT GTCCTGCGCT GCCTACTAGC CTACTTCCTT 
GACAAGAGTG CAGAGGAGAT GCCATACCTG AAATGCCCAC TTCACACAGT CCTGAAACTG 
ACGCCCGTGG CGTATGGGTG CCGTGTGGAG TCCATTTACT TGAATGTAGA ATCAGTGAGC 
ACGCATCGGG AGAGGTCAGA GGCTGTCAAA ATACAACACT TTGCCAGTGT AGTCAGACCT 
TCCTCTTACA CCGAACTTGA CTTTCTGTCT GTGGAGTCCG CAAAACAGGA TGCAAAGAAG 
GGACCTAACC CTCTCATGAG ACGCAATAGT GTCACCCCAC TAGCCAGCCC CGAGCCCACC 
AAAAAGCCTC GCATCAACAG CTTTGAGGAG CATGTGGCTT CCACTTCTGC TGCCCTGCCA 
AGCTGCCTGC CCCCGGAAGT GCCCACGCAG CTGCCCGGAC AAAACATGAG GAGCCCCCGG 
AGCGGTGCCG AATCTTCCCA GAAGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pfkfb3 ( XM_006254193.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006254255.1 Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa38017
Clone ID Related Accession (Same CDS sequence)	XM_006254193.3
Accession Version	XM_006254193.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006254193.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTGG AACTGACCCA GAGCCGGGTG CAGAAGATCT GGGTGCCTGT CGATCACCGA 
CCCTCGTTGC CCAGATCCTG TGGGCCGAAG CTGACCAACT CCCCAACGGT GATTGTGATG 
GTGGGGCTCC CAGCCCGGGG TAAGACTTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC 
TGGATAGGTG TTCCAACGAA AGTGTTCAAT GTGGGAGAAT ACCGACGGGA GGCTGTGAAG 
CAATACAGCT CTTACAACTT CTTCCGCCCT GACAATGAGG AGGCCATGAG AGTCCGAAAA 
CAGTGTGCCT TAGCTGCCCT GAGAGACGTC AAAAGCTACC TGACAAAGGA AGGAGGACAG 
ATTGCAGTTT TTGATGCCAC CAATACAACG AGAGAAAGGA GGCACATGAT CCTTCATTTT 
GCCAAAGAAA ATGACTTCAA GGCATTTTTC ATCGAGTCCG TCTGTGACGA CCCTACAGTT 
GTGGCCTCCA ATATCATGGA AGTGAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCC 
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAACTGCT ACGAAGCCAG CTACCAGCCT 
CTTGACCCTG ATAAATGTGA CAGGGACCTG TCATTCATCA AAGTGATTGA CGTTGGCCGG 
AGGTTCTTGG TGAACAGAGT GCAGGACCAC ATCCAGAGCC GAATCGTGTA TTACCTGATG 
AACATCCACG TGCAGCCCCG GACCATCTAC CTGTGTCGGC ACGGCGAGAA TGAGTACAAC 
GTGCAGGGCA AGATTGGGGG CGACTCAGGC CTGTCCAGCA GAGGCAAGAA GTTCGCCAAT 
GCCCTGAGCA AGTTCGTTGA AGAGCAGAAT CTGAAGGACC TCCGCGTGTG GACCAGTCAG 
CTGAAAAGTA CCATCCAGAC CGCGGAGGCC CTAAGGCTCC CCTACGAGCA GTGGAAGGCG 
CTCAATGAGA TTGATGCTGG TGTGTGTGAG GAACTAACCT ATGAGGAGAT CAGAGACACC 
TACCCTGAGG AGTATGCACT GCGTGAGCAA GACAAATACT ACTACCGCTA TCCCACAGGC 
GAGTCCTACC AGGATCTTGT CCAGCGCCTG GAGCCTGTGA TCATGGAATT AGAGCGCCAA 
GAGAACGTAT TGGTCATCTG TCACCAGGCT GTCCTGCGCT GCCTACTAGC CTACTTCCTT 
GACAAGAGTG CAGAGGAGAT GCCATACCTG AAATGCCCAC TTCACACAGT CCTGAAACTG 
ACGCCCGTGG CGTATGGGTG CCGTGTGGAG TCCATTTACT TGAATGTAGA ATCAGTGAGC 
ACGCATCGGG AGAGGTCAGA GGCTGTCAAA ATACAACACT TTGCCAGTGT AGTCAGACCT 
TCCTCTTACA CCGAACTTGA CTTTCTGTCT GTGGAGTCCG CAAAACAGGA TGCAAAGAAG 
GGACCTAACC CTCTCATGAG ACGCAATAGT GTCACCCCAC TAGCCAGCCC CGAGCCCACC 
AAAAAGCCTC GCATCAACAG CTTTGAGGAG CATGTGGCTT CCACTTCTGC TGCCCTGCCA 
AGCTGCCTGC CCCCGGAAGT GCCCACGCAG CTGCCCGGAC AACCTTTGCT AGGGAAAGCC 
TGTCTAACAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006254255.1
CDS	401..2032
Translation	MPLELTQSRVQKIWVPVDHRPSLPRSCGPKLTNSPTVIVMVGLPARGKTYISKKLTRYLNWIGVPTKVFNVGEYRREAVKQYSSYNFFRPDNEEAMRVRKQCALAALRDVKSYLTKEGGQIAVFDATNTTRERRHMILHFAKENDFKAFFIESVCDDPTVVASNIMEVKISSPDYKDCNSAEAMDDFMKRINCYEASYQPLDPDKCDRDLSFIKVIDVGRRFLVNRVQDHIQSRIVYYLMNIHVQPRTIYLCRHGENEYNVQGKIGGDSGLSSRGKKFANALSKFVEEQNLKDLRVWTSQLKSTIQTAEALRLPYEQWKALNEIDAGVCEELTYEEIRDTYPEEYALREQDKYYYRYPTGESYQDLVQRLEPVIMELERQENVLVICHQAVLRCLLAYFLDKSAEEMPYLKCPLHTVLKLTPVAYGCRVESIYLNVESVSTHRERSEAVKIQHFASVVRPSSYTELDFLSVESAKQDAKKGPNPLMRRNSVTPLASPEPTKKPRINSFEEHVASTSAALPSCLPPEVPTQLPGQPLLGKACLT

Target ORF information:

RefSeq Version	XM_006254193.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006254193.3

ORF Insert Sequence:

ATGCCTTTGG AACTGACCCA GAGCCGGGTG CAGAAGATCT GGGTGCCTGT CGATCACCGA 
CCCTCGTTGC CCAGATCCTG TGGGCCGAAG CTGACCAACT CCCCAACGGT GATTGTGATG 
GTGGGGCTCC CAGCCCGGGG TAAGACTTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC 
TGGATAGGTG TTCCAACGAA AGTGTTCAAT GTGGGAGAAT ACCGACGGGA GGCTGTGAAG 
CAATACAGCT CTTACAACTT CTTCCGCCCT GACAATGAGG AGGCCATGAG AGTCCGAAAA 
CAGTGTGCCT TAGCTGCCCT GAGAGACGTC AAAAGCTACC TGACAAAGGA AGGAGGACAG 
ATTGCAGTTT TTGATGCCAC CAATACAACG AGAGAAAGGA GGCACATGAT CCTTCATTTT 
GCCAAAGAAA ATGACTTCAA GGCATTTTTC ATCGAGTCCG TCTGTGACGA CCCTACAGTT 
GTGGCCTCCA ATATCATGGA AGTGAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCC 
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAACTGCT ACGAAGCCAG CTACCAGCCT 
CTTGACCCTG ATAAATGTGA CAGGGACCTG TCATTCATCA AAGTGATTGA CGTTGGCCGG 
AGGTTCTTGG TGAACAGAGT GCAGGACCAC ATCCAGAGCC GAATCGTGTA TTACCTGATG 
AACATCCACG TGCAGCCCCG GACCATCTAC CTGTGTCGGC ACGGCGAGAA TGAGTACAAC 
GTGCAGGGCA AGATTGGGGG CGACTCAGGC CTGTCCAGCA GAGGCAAGAA GTTCGCCAAT 
GCCCTGAGCA AGTTCGTTGA AGAGCAGAAT CTGAAGGACC TCCGCGTGTG GACCAGTCAG 
CTGAAAAGTA CCATCCAGAC CGCGGAGGCC CTAAGGCTCC CCTACGAGCA GTGGAAGGCG 
CTCAATGAGA TTGATGCTGG TGTGTGTGAG GAACTAACCT ATGAGGAGAT CAGAGACACC 
TACCCTGAGG AGTATGCACT GCGTGAGCAA GACAAATACT ACTACCGCTA TCCCACAGGC 
GAGTCCTACC AGGATCTTGT CCAGCGCCTG GAGCCTGTGA TCATGGAATT AGAGCGCCAA 
GAGAACGTAT TGGTCATCTG TCACCAGGCT GTCCTGCGCT GCCTACTAGC CTACTTCCTT 
GACAAGAGTG CAGAGGAGAT GCCATACCTG AAATGCCCAC TTCACACAGT CCTGAAACTG 
ACGCCCGTGG CGTATGGGTG CCGTGTGGAG TCCATTTACT TGAATGTAGA ATCAGTGAGC 
ACGCATCGGG AGAGGTCAGA GGCTGTCAAA ATACAACACT TTGCCAGTGT AGTCAGACCT 
TCCTCTTACA CCGAACTTGA CTTTCTGTCT GTGGAGTCCG CAAAACAGGA TGCAAAGAAG 
GGACCTAACC CTCTCATGAG ACGCAATAGT GTCACCCCAC TAGCCAGCCC CGAGCCCACC 
AAAAAGCCTC GCATCAACAG CTTTGAGGAG CATGTGGCTT CCACTTCTGC TGCCCTGCCA 
AGCTGCCTGC CCCCGGAAGT GCCCACGCAG CTGCCCGGAC AACCTTTGCT AGGGAAAGCC 
TGTCTAACAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pfkfb3 ( XM_006254196.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006254258.1 Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3), transcript variant X4, mRNA.

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CloneID	ORa38019
Clone ID Related Accession (Same CDS sequence)	XM_006254196.3
Accession Version	XM_006254196.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006254196.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTGG AACTGACCCA GAGCCGGGTG CAGAAGATCT GGGTGCCTGT CGATCACCGA 
CCCTCGTTGC CCAGATCCTG TGGGCCGAAG CTGACCAACT CCCCAACGGT GATTGTGATG 
GTGGGGCTCC CAGCCCGGGG TAAGACTTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC 
TGGATAGGTG TTCCAACGAA AGTGTTCAAT GTGGGAGAAT ACCGACGGGA GGCTGTGAAG 
CAATACAGCT CTTACAACTT CTTCCGCCCT GACAATGAGG AGGCCATGAG AGTCCGAAAA 
CAGTGTGCCT TAGCTGCCCT GAGAGACGTC AAAAGCTACC TGACAAAGGA AGGAGGACAG 
ATTGCAGTTT TTGATGCCAC CAATACAACG AGAGAAAGGA GGCACATGAT CCTTCATTTT 
GCCAAAGAAA ATGACTTCAA GGCATTTTTC ATCGAGTCCG TCTGTGACGA CCCTACAGTT 
GTGGCCTCCA ATATCATGGA AGTGAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCC 
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAACTGCT ACGAAGCCAG CTACCAGCCT 
CTTGACCCTG ATAAATGTGA CAGGGACCTG TCATTCATCA AAGTGATTGA CGTTGGCCGG 
AGGTTCTTGG TGAACAGAGT GCAGGACCAC ATCCAGAGCC GAATCGTGTA TTACCTGATG 
AACATCCACG TGCAGCCCCG GACCATCTAC CTGTGTCGGC ACGGCGAGAA TGAGTACAAC 
GTGCAGGGCA AGATTGGGGG CGACTCAGGC CTGTCCAGCA GAGGCAAGAA GTTCGCCAAT 
GCCCTGAGCA AGTTCGTTGA AGAGCAGAAT CTGAAGGACC TCCGCGTGTG GACCAGTCAG 
CTGAAAAGTA CCATCCAGAC CGCGGAGGCC CTAAGGCTCC CCTACGAGCA GTGGAAGGCG 
CTCAATGAGA TTGATGCTGG TGTGTGTGAG GAACTAACCT ATGAGGAGAT CAGAGACACC 
TACCCTGAGG AGTATGCACT GCGTGAGCAA GACAAATACT ACTACCGCTA TCCCACAGGC 
GAGTCCTACC AGGATCTTGT CCAGCGCCTG GAGCCTGTGA TCATGGAATT AGAGCGCCAA 
GAGAACGTAT TGGTCATCTG TCACCAGGCT GTCCTGCGCT GCCTACTAGC CTACTTCCTT 
GACAAGAGTG CAGAGGAGAT GCCATACCTG AAATGCCCAC TTCACACAGT CCTGAAACTG 
ACGCCCGTGG CGTATGGGTG CCGTGTGGAG TCCATTTACT TGAATGTAGA ATCAGTGAGC 
ACGCATCGGG AGAGGTCAGA GGATGCAAAG AAGGGACCTA ACCCTCTCAT GAGACGCAAT 
AGTGTCACCC CACTAGCCAG CCCCGAGCCC ACCAAAAAGC CTCGCATCAA CAGCTTTGAG 
GAGCATGTGG CTTCCACTTC TGCTGCCCTG CCAAGCTGCC TGCCCCCGGA AGTGCCCACG 
CAGCTGCCCG GACAACCTTT GCTAGGGAAA GCCTGTCTAC GAACTGTCTG TCACATTTTT 
TCAAAGTTTT CTCCTTACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006254258.1
CDS	401..1981
Translation	MPLELTQSRVQKIWVPVDHRPSLPRSCGPKLTNSPTVIVMVGLPARGKTYISKKLTRYLNWIGVPTKVFNVGEYRREAVKQYSSYNFFRPDNEEAMRVRKQCALAALRDVKSYLTKEGGQIAVFDATNTTRERRHMILHFAKENDFKAFFIESVCDDPTVVASNIMEVKISSPDYKDCNSAEAMDDFMKRINCYEASYQPLDPDKCDRDLSFIKVIDVGRRFLVNRVQDHIQSRIVYYLMNIHVQPRTIYLCRHGENEYNVQGKIGGDSGLSSRGKKFANALSKFVEEQNLKDLRVWTSQLKSTIQTAEALRLPYEQWKALNEIDAGVCEELTYEEIRDTYPEEYALREQDKYYYRYPTGESYQDLVQRLEPVIMELERQENVLVICHQAVLRCLLAYFLDKSAEEMPYLKCPLHTVLKLTPVAYGCRVESIYLNVESVSTHRERSEDAKKGPNPLMRRNSVTPLASPEPTKKPRINSFEEHVASTSAALPSCLPPEVPTQLPGQPLLGKACLRTVCHIFSKFSPY

Target ORF information:

RefSeq Version	XM_006254196.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006254196.3

ORF Insert Sequence:

ATGCCTTTGG AACTGACCCA GAGCCGGGTG CAGAAGATCT GGGTGCCTGT CGATCACCGA 
CCCTCGTTGC CCAGATCCTG TGGGCCGAAG CTGACCAACT CCCCAACGGT GATTGTGATG 
GTGGGGCTCC CAGCCCGGGG TAAGACTTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC 
TGGATAGGTG TTCCAACGAA AGTGTTCAAT GTGGGAGAAT ACCGACGGGA GGCTGTGAAG 
CAATACAGCT CTTACAACTT CTTCCGCCCT GACAATGAGG AGGCCATGAG AGTCCGAAAA 
CAGTGTGCCT TAGCTGCCCT GAGAGACGTC AAAAGCTACC TGACAAAGGA AGGAGGACAG 
ATTGCAGTTT TTGATGCCAC CAATACAACG AGAGAAAGGA GGCACATGAT CCTTCATTTT 
GCCAAAGAAA ATGACTTCAA GGCATTTTTC ATCGAGTCCG TCTGTGACGA CCCTACAGTT 
GTGGCCTCCA ATATCATGGA AGTGAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCC 
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAACTGCT ACGAAGCCAG CTACCAGCCT 
CTTGACCCTG ATAAATGTGA CAGGGACCTG TCATTCATCA AAGTGATTGA CGTTGGCCGG 
AGGTTCTTGG TGAACAGAGT GCAGGACCAC ATCCAGAGCC GAATCGTGTA TTACCTGATG 
AACATCCACG TGCAGCCCCG GACCATCTAC CTGTGTCGGC ACGGCGAGAA TGAGTACAAC 
GTGCAGGGCA AGATTGGGGG CGACTCAGGC CTGTCCAGCA GAGGCAAGAA GTTCGCCAAT 
GCCCTGAGCA AGTTCGTTGA AGAGCAGAAT CTGAAGGACC TCCGCGTGTG GACCAGTCAG 
CTGAAAAGTA CCATCCAGAC CGCGGAGGCC CTAAGGCTCC CCTACGAGCA GTGGAAGGCG 
CTCAATGAGA TTGATGCTGG TGTGTGTGAG GAACTAACCT ATGAGGAGAT CAGAGACACC 
TACCCTGAGG AGTATGCACT GCGTGAGCAA GACAAATACT ACTACCGCTA TCCCACAGGC 
GAGTCCTACC AGGATCTTGT CCAGCGCCTG GAGCCTGTGA TCATGGAATT AGAGCGCCAA 
GAGAACGTAT TGGTCATCTG TCACCAGGCT GTCCTGCGCT GCCTACTAGC CTACTTCCTT 
GACAAGAGTG CAGAGGAGAT GCCATACCTG AAATGCCCAC TTCACACAGT CCTGAAACTG 
ACGCCCGTGG CGTATGGGTG CCGTGTGGAG TCCATTTACT TGAATGTAGA ATCAGTGAGC 
ACGCATCGGG AGAGGTCAGA GGATGCAAAG AAGGGACCTA ACCCTCTCAT GAGACGCAAT 
AGTGTCACCC CACTAGCCAG CCCCGAGCCC ACCAAAAAGC CTCGCATCAA CAGCTTTGAG 
GAGCATGTGG CTTCCACTTC TGCTGCCCTG CCAAGCTGCC TGCCCCCGGA AGTGCCCACG 
CAGCTGCCCG GACAACCTTT GCTAGGGAAA GCCTGTCTAC GAACTGTCTG TCACATTTTT 
TCAAAGTTTT CTCCTTACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pfkfb3 ( XM_006254197.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006254259.1 Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa38020
Clone ID Related Accession (Same CDS sequence)	XM_006254197.3
Accession Version	XM_006254197.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006254197.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTGG AACTGACCCA GAGCCGGGTG CAGAAGATCT GGGTGCCTGT CGATCACCGA 
CCCTCGTTGC CCAGATCCTG TGGGCCGAAG CTGACCAACT CCCCAACGGT GATTGTGATG 
GTGGGGCTCC CAGCCCGGGG TAAGACTTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC 
TGGATAGGTG TTCCAACGAA AGTGTTCAAT GTGGGAGAAT ACCGACGGGA GGCTGTGAAG 
CAATACAGCT CTTACAACTT CTTCCGCCCT GACAATGAGG AGGCCATGAG AGTCCGAAAA 
CAGTGTGCCT TAGCTGCCCT GAGAGACGTC AAAAGCTACC TGACAAAGGA AGGAGGACAG 
ATTGCAGTTT TTGATGCCAC CAATACAACG AGAGAAAGGA GGCACATGAT CCTTCATTTT 
GCCAAAGAAA ATGACTTCAA GGCATTTTTC ATCGAGTCCG TCTGTGACGA CCCTACAGTT 
GTGGCCTCCA ATATCATGGA AGTGAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCC 
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAACTGCT ACGAAGCCAG CTACCAGCCT 
CTTGACCCTG ATAAATGTGA CAGGGACCTG TCATTCATCA AAGTGATTGA CGTTGGCCGG 
AGGTTCTTGG TGAACAGAGT GCAGGACCAC ATCCAGAGCC GAATCGTGTA TTACCTGATG 
AACATCCACG TGCAGCCCCG GACCATCTAC CTGTGTCGGC ACGGCGAGAA TGAGTACAAC 
GTGCAGGGCA AGATTGGGGG CGACTCAGGC CTGTCCAGCA GAGGCAAGAA GTTCGCCAAT 
GCCCTGAGCA AGTTCGTTGA AGAGCAGAAT CTGAAGGACC TCCGCGTGTG GACCAGTCAG 
CTGAAAAGTA CCATCCAGAC CGCGGAGGCC CTAAGGCTCC CCTACGAGCA GTGGAAGGCG 
CTCAATGAGA TTGATGCTGG TGTGTGTGAG GAACTAACCT ATGAGGAGAT CAGAGACACC 
TACCCTGAGG AGTATGCACT GCGTGAGCAA GACAAATACT ACTACCGCTA TCCCACAGGC 
GAGTCCTACC AGGATCTTGT CCAGCGCCTG GAGCCTGTGA TCATGGAATT AGAGCGCCAA 
GAGAACGTAT TGGTCATCTG TCACCAGGCT GTCCTGCGCT GCCTACTAGC CTACTTCCTT 
GACAAGAGTG CAGAGGAGAT GCCATACCTG AAATGCCCAC TTCACACAGT CCTGAAACTG 
ACGCCCGTGG CGTATGGGTG CCGTGTGGAG TCCATTTACT TGAATGTAGA ATCAGTGAGC 
ACGCATCGGG AGAGGTCAGA GGATGCAAAG AAGGGACCTA ACCCTCTCAT GAGACGCAAT 
AGTGTCACCC CACTAGCCAG CCCCGAGCCC ACCAAAAAGC CTCGCATCAA CAGCTTTGAG 
GAGCATGTGG CTTCCACTTC TGCTGCCCTG CCAAGCTGCC TGCCCCCGGA AGTGCCCACG 
CAGCTGCCCG GACAAAACAT GAGGAGCCCC CGGAGCGGTG CCGAATCTTC CCAGAAGCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006254259.1
CDS	401..1963
Translation	MPLELTQSRVQKIWVPVDHRPSLPRSCGPKLTNSPTVIVMVGLPARGKTYISKKLTRYLNWIGVPTKVFNVGEYRREAVKQYSSYNFFRPDNEEAMRVRKQCALAALRDVKSYLTKEGGQIAVFDATNTTRERRHMILHFAKENDFKAFFIESVCDDPTVVASNIMEVKISSPDYKDCNSAEAMDDFMKRINCYEASYQPLDPDKCDRDLSFIKVIDVGRRFLVNRVQDHIQSRIVYYLMNIHVQPRTIYLCRHGENEYNVQGKIGGDSGLSSRGKKFANALSKFVEEQNLKDLRVWTSQLKSTIQTAEALRLPYEQWKALNEIDAGVCEELTYEEIRDTYPEEYALREQDKYYYRYPTGESYQDLVQRLEPVIMELERQENVLVICHQAVLRCLLAYFLDKSAEEMPYLKCPLHTVLKLTPVAYGCRVESIYLNVESVSTHRERSEDAKKGPNPLMRRNSVTPLASPEPTKKPRINSFEEHVASTSAALPSCLPPEVPTQLPGQNMRSPRSGAESSQKH

Target ORF information:

RefSeq Version	XM_006254197.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006254197.3

ORF Insert Sequence:

ATGCCTTTGG AACTGACCCA GAGCCGGGTG CAGAAGATCT GGGTGCCTGT CGATCACCGA 
CCCTCGTTGC CCAGATCCTG TGGGCCGAAG CTGACCAACT CCCCAACGGT GATTGTGATG 
GTGGGGCTCC CAGCCCGGGG TAAGACTTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC 
TGGATAGGTG TTCCAACGAA AGTGTTCAAT GTGGGAGAAT ACCGACGGGA GGCTGTGAAG 
CAATACAGCT CTTACAACTT CTTCCGCCCT GACAATGAGG AGGCCATGAG AGTCCGAAAA 
CAGTGTGCCT TAGCTGCCCT GAGAGACGTC AAAAGCTACC TGACAAAGGA AGGAGGACAG 
ATTGCAGTTT TTGATGCCAC CAATACAACG AGAGAAAGGA GGCACATGAT CCTTCATTTT 
GCCAAAGAAA ATGACTTCAA GGCATTTTTC ATCGAGTCCG TCTGTGACGA CCCTACAGTT 
GTGGCCTCCA ATATCATGGA AGTGAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCC 
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAACTGCT ACGAAGCCAG CTACCAGCCT 
CTTGACCCTG ATAAATGTGA CAGGGACCTG TCATTCATCA AAGTGATTGA CGTTGGCCGG 
AGGTTCTTGG TGAACAGAGT GCAGGACCAC ATCCAGAGCC GAATCGTGTA TTACCTGATG 
AACATCCACG TGCAGCCCCG GACCATCTAC CTGTGTCGGC ACGGCGAGAA TGAGTACAAC 
GTGCAGGGCA AGATTGGGGG CGACTCAGGC CTGTCCAGCA GAGGCAAGAA GTTCGCCAAT 
GCCCTGAGCA AGTTCGTTGA AGAGCAGAAT CTGAAGGACC TCCGCGTGTG GACCAGTCAG 
CTGAAAAGTA CCATCCAGAC CGCGGAGGCC CTAAGGCTCC CCTACGAGCA GTGGAAGGCG 
CTCAATGAGA TTGATGCTGG TGTGTGTGAG GAACTAACCT ATGAGGAGAT CAGAGACACC 
TACCCTGAGG AGTATGCACT GCGTGAGCAA GACAAATACT ACTACCGCTA TCCCACAGGC 
GAGTCCTACC AGGATCTTGT CCAGCGCCTG GAGCCTGTGA TCATGGAATT AGAGCGCCAA 
GAGAACGTAT TGGTCATCTG TCACCAGGCT GTCCTGCGCT GCCTACTAGC CTACTTCCTT 
GACAAGAGTG CAGAGGAGAT GCCATACCTG AAATGCCCAC TTCACACAGT CCTGAAACTG 
ACGCCCGTGG CGTATGGGTG CCGTGTGGAG TCCATTTACT TGAATGTAGA ATCAGTGAGC 
ACGCATCGGG AGAGGTCAGA GGATGCAAAG AAGGGACCTA ACCCTCTCAT GAGACGCAAT 
AGTGTCACCC CACTAGCCAG CCCCGAGCCC ACCAAAAAGC CTCGCATCAA CAGCTTTGAG 
GAGCATGTGG CTTCCACTTC TGCTGCCCTG CCAAGCTGCC TGCCCCCGGA AGTGCCCACG 
CAGCTGCCCG GACAAAACAT GAGGAGCCCC CGGAGCGGTG CCGAATCTTC CCAGAAGCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pfkfb3 ( XM_006254198.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006254260.1 Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa38021
Clone ID Related Accession (Same CDS sequence)	XM_006254198.3
Accession Version	XM_006254198.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006254198.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTGG AACTGACCCA GAGCCGGGTG CAGAAGATCT GGGTGCCTGT CGATCACCGA 
CCCTCGTTGC CCAGATCCTG TGGGCCGAAG CTGACCAACT CCCCAACGGT GATTGTGATG 
GTGGGGCTCC CAGCCCGGGG TAAGACTTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC 
TGGATAGGTG TTCCAACGAA AGTGTTCAAT GTGGGAGAAT ACCGACGGGA GGCTGTGAAG 
CAATACAGCT CTTACAACTT CTTCCGCCCT GACAATGAGG AGGCCATGAG AGTCCGAAAA 
CAGTGTGCCT TAGCTGCCCT GAGAGACGTC AAAAGCTACC TGACAAAGGA AGGAGGACAG 
ATTGCAGTTT TTGATGCCAC CAATACAACG AGAGAAAGGA GGCACATGAT CCTTCATTTT 
GCCAAAGAAA ATGACTTCAA GGCATTTTTC ATCGAGTCCG TCTGTGACGA CCCTACAGTT 
GTGGCCTCCA ATATCATGGA AGTGAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCC 
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAACTGCT ACGAAGCCAG CTACCAGCCT 
CTTGACCCTG ATAAATGTGA CAGGGACCTG TCATTCATCA AAGTGATTGA CGTTGGCCGG 
AGGTTCTTGG TGAACAGAGT GCAGGACCAC ATCCAGAGCC GAATCGTGTA TTACCTGATG 
AACATCCACG TGCAGCCCCG GACCATCTAC CTGTGTCGGC ACGGCGAGAA TGAGTACAAC 
GTGCAGGGCA AGATTGGGGG CGACTCAGGC CTGTCCAGCA GAGGCAAGAA GTTCGCCAAT 
GCCCTGAGCA AGTTCGTTGA AGAGCAGAAT CTGAAGGACC TCCGCGTGTG GACCAGTCAG 
CTGAAAAGTA CCATCCAGAC CGCGGAGGCC CTAAGGCTCC CCTACGAGCA GTGGAAGGCG 
CTCAATGAGA TTGATGCTGG TGTGTGTGAG GAACTAACCT ATGAGGAGAT CAGAGACACC 
TACCCTGAGG AGTATGCACT GCGTGAGCAA GACAAATACT ACTACCGCTA TCCCACAGGC 
GAGTCCTACC AGGATCTTGT CCAGCGCCTG GAGCCTGTGA TCATGGAATT AGAGCGCCAA 
GAGAACGTAT TGGTCATCTG TCACCAGGCT GTCCTGCGCT GCCTACTAGC CTACTTCCTT 
GACAAGAGTG CAGAGGAGAT GCCATACCTG AAATGCCCAC TTCACACAGT CCTGAAACTG 
ACGCCCGTGG CGTATGGGTG CCGTGTGGAG TCCATTTACT TGAATGTAGA ATCAGTGAGC 
ACGCATCGGG AGAGGTCAGA GGATGCAAAG AAGGGACCTA ACCCTCTCAT GAGACGCAAT 
AGTGTCACCC CACTAGCCAG CCCCGAGCCC ACCAAAAAGC CTCGCATCAA CAGCTTTGAG 
GAGCATGTGG CTTCCACTTC TGCTGCCCTG CCAAGCTGCC TGCCCCCGGA AGTGCCCACG 
CAGCTGCCCG GACAACCTTT GCTAGGGAAA GCCTGTCTAA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006254260.1
CDS	401..1945
Translation	MPLELTQSRVQKIWVPVDHRPSLPRSCGPKLTNSPTVIVMVGLPARGKTYISKKLTRYLNWIGVPTKVFNVGEYRREAVKQYSSYNFFRPDNEEAMRVRKQCALAALRDVKSYLTKEGGQIAVFDATNTTRERRHMILHFAKENDFKAFFIESVCDDPTVVASNIMEVKISSPDYKDCNSAEAMDDFMKRINCYEASYQPLDPDKCDRDLSFIKVIDVGRRFLVNRVQDHIQSRIVYYLMNIHVQPRTIYLCRHGENEYNVQGKIGGDSGLSSRGKKFANALSKFVEEQNLKDLRVWTSQLKSTIQTAEALRLPYEQWKALNEIDAGVCEELTYEEIRDTYPEEYALREQDKYYYRYPTGESYQDLVQRLEPVIMELERQENVLVICHQAVLRCLLAYFLDKSAEEMPYLKCPLHTVLKLTPVAYGCRVESIYLNVESVSTHRERSEDAKKGPNPLMRRNSVTPLASPEPTKKPRINSFEEHVASTSAALPSCLPPEVPTQLPGQPLLGKACLT

Target ORF information:

RefSeq Version	XM_006254198.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006254198.3

ORF Insert Sequence:

ATGCCTTTGG AACTGACCCA GAGCCGGGTG CAGAAGATCT GGGTGCCTGT CGATCACCGA 
CCCTCGTTGC CCAGATCCTG TGGGCCGAAG CTGACCAACT CCCCAACGGT GATTGTGATG 
GTGGGGCTCC CAGCCCGGGG TAAGACTTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC 
TGGATAGGTG TTCCAACGAA AGTGTTCAAT GTGGGAGAAT ACCGACGGGA GGCTGTGAAG 
CAATACAGCT CTTACAACTT CTTCCGCCCT GACAATGAGG AGGCCATGAG AGTCCGAAAA 
CAGTGTGCCT TAGCTGCCCT GAGAGACGTC AAAAGCTACC TGACAAAGGA AGGAGGACAG 
ATTGCAGTTT TTGATGCCAC CAATACAACG AGAGAAAGGA GGCACATGAT CCTTCATTTT 
GCCAAAGAAA ATGACTTCAA GGCATTTTTC ATCGAGTCCG TCTGTGACGA CCCTACAGTT 
GTGGCCTCCA ATATCATGGA AGTGAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCC 
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAACTGCT ACGAAGCCAG CTACCAGCCT 
CTTGACCCTG ATAAATGTGA CAGGGACCTG TCATTCATCA AAGTGATTGA CGTTGGCCGG 
AGGTTCTTGG TGAACAGAGT GCAGGACCAC ATCCAGAGCC GAATCGTGTA TTACCTGATG 
AACATCCACG TGCAGCCCCG GACCATCTAC CTGTGTCGGC ACGGCGAGAA TGAGTACAAC 
GTGCAGGGCA AGATTGGGGG CGACTCAGGC CTGTCCAGCA GAGGCAAGAA GTTCGCCAAT 
GCCCTGAGCA AGTTCGTTGA AGAGCAGAAT CTGAAGGACC TCCGCGTGTG GACCAGTCAG 
CTGAAAAGTA CCATCCAGAC CGCGGAGGCC CTAAGGCTCC CCTACGAGCA GTGGAAGGCG 
CTCAATGAGA TTGATGCTGG TGTGTGTGAG GAACTAACCT ATGAGGAGAT CAGAGACACC 
TACCCTGAGG AGTATGCACT GCGTGAGCAA GACAAATACT ACTACCGCTA TCCCACAGGC 
GAGTCCTACC AGGATCTTGT CCAGCGCCTG GAGCCTGTGA TCATGGAATT AGAGCGCCAA 
GAGAACGTAT TGGTCATCTG TCACCAGGCT GTCCTGCGCT GCCTACTAGC CTACTTCCTT 
GACAAGAGTG CAGAGGAGAT GCCATACCTG AAATGCCCAC TTCACACAGT CCTGAAACTG 
ACGCCCGTGG CGTATGGGTG CCGTGTGGAG TCCATTTACT TGAATGTAGA ATCAGTGAGC 
ACGCATCGGG AGAGGTCAGA GGATGCAAAG AAGGGACCTA ACCCTCTCAT GAGACGCAAT 
AGTGTCACCC CACTAGCCAG CCCCGAGCCC ACCAAAAAGC CTCGCATCAA CAGCTTTGAG 
GAGCATGTGG CTTCCACTTC TGCTGCCCTG CCAAGCTGCC TGCCCCCGGA AGTGCCCACG 
CAGCTGCCCG GACAACCTTT GCTAGGGAAA GCCTGTCTAA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pfkfb3 ( XM_017600444.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455933.1 Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3), transcript variant X3, mRNA.

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CloneID	ORa58989
Clone ID Related Accession (Same CDS sequence)	XM_017600444.1
Accession Version	XM_017600444.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTTCA GGAAAGCCTG TGGGCCGAAG CTGACCAACT CCCCAACGGT GATTGTGATG 
GTGGGGCTCC CAGCCCGGGG TAAGACTTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC 
TGGATAGGTG TTCCAACGAA AGTGTTCAAT GTGGGAGAAT ACCGACGGGA GGCTGTGAAG 
CAATACAGCT CTTACAACTT CTTCCGCCCT GACAATGAGG AGGCCATGAG AGTCCGAAAA 
CAGTGTGCCT TAGCTGCCCT GAGAGACGTC AAAAGCTACC TGACAAAGGA AGGAGGACAG 
ATTGCAGTTT TTGATGCCAC CAATACAACG AGAGAAAGGA GGCACATGAT CCTTCATTTT 
GCCAAAGAAA ATGACTTCAA GGCATTTTTC ATCGAGTCCG TCTGTGACGA CCCTACAGTT 
GTGGCCTCCA ATATCATGGA AGTGAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCC 
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAACTGCT ACGAAGCCAG CTACCAGCCT 
CTTGACCCTG ATAAATGTGA CAGGGACCTG TCATTCATCA AAGTGATTGA CGTTGGCCGG 
AGGTTCTTGG TGAACAGAGT GCAGGACCAC ATCCAGAGCC GAATCGTGTA TTACCTGATG 
AACATCCACG TGCAGCCCCG GACCATCTAC CTGTGTCGGC ACGGCGAGAA TGAGTACAAC 
GTGCAGGGCA AGATTGGGGG CGACTCAGGC CTGTCCAGCA GAGGCAAGAA GTTCGCCAAT 
GCCCTGAGCA AGTTCGTTGA AGAGCAGAAT CTGAAGGACC TCCGCGTGTG GACCAGTCAG 
CTGAAAAGTA CCATCCAGAC CGCGGAGGCC CTAAGGCTCC CCTACGAGCA GTGGAAGGCG 
CTCAATGAGA TTGATGCTGG TGTGTGTGAG GAACTAACCT ATGAGGAGAT CAGAGACACC 
TACCCTGAGG AGTATGCACT GCGTGAGCAA GACAAATACT ACTACCGCTA TCCCACAGGC 
GAGTCCTACC AGGATCTTGT CCAGCGCCTG GAGCCTGTGA TCATGGAATT AGAGCGCCAA 
GAGAACGTAT TGGTCATCTG TCACCAGGCT GTCCTGCGCT GCCTACTAGC CTACTTCCTT 
GACAAGAGTG CAGAGGAGAT GCCATACCTG AAATGCCCAC TTCACACAGT CCTGAAACTG 
ACGCCCGTGG CGTATGGGTG CCGTGTGGAG TCCATTTACT TGAATGTAGA ATCAGTGAGC 
ACGCATCGGG AGAGGTCAGA GGCTGTCAAA ATACAACACT TTGCCAGTGT AGTCAGACCT 
TCCTCTTACA CCGAACTTGA CTTTCTGTCT GTGGAGTCCG CAAAACAGGA TGCAAAGAAG 
GGACCTAACC CTCTCATGAG ACGCAATAGT GTCACCCCAC TAGCCAGCCC CGAGCCCACC 
AAAAAGCCTC GCATCAACAG CTTTGAGGAG CATGTGGCTT CCACTTCTGC TGCCCTGCCA 
AGCTGCCTGC CCCCGGAAGT GCCCACGCAG CTGCCCGGAC AACCTTTGCT AGGGAAAGCC 
TGTCTACGAA CTGTCTGTCA CATTTTTTCA AAGTTTTCTC CTTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455933.1
CDS	105..1712
Translation	MPFRKACGPKLTNSPTVIVMVGLPARGKTYISKKLTRYLNWIGVPTKVFNVGEYRREAVKQYSSYNFFRPDNEEAMRVRKQCALAALRDVKSYLTKEGGQIAVFDATNTTRERRHMILHFAKENDFKAFFIESVCDDPTVVASNIMEVKISSPDYKDCNSAEAMDDFMKRINCYEASYQPLDPDKCDRDLSFIKVIDVGRRFLVNRVQDHIQSRIVYYLMNIHVQPRTIYLCRHGENEYNVQGKIGGDSGLSSRGKKFANALSKFVEEQNLKDLRVWTSQLKSTIQTAEALRLPYEQWKALNEIDAGVCEELTYEEIRDTYPEEYALREQDKYYYRYPTGESYQDLVQRLEPVIMELERQENVLVICHQAVLRCLLAYFLDKSAEEMPYLKCPLHTVLKLTPVAYGCRVESIYLNVESVSTHRERSEAVKIQHFASVVRPSSYTELDFLSVESAKQDAKKGPNPLMRRNSVTPLASPEPTKKPRINSFEEHVASTSAALPSCLPPEVPTQLPGQPLLGKACLRTVCHIFSKFSPY

Target ORF information:

RefSeq Version	XM_017600444.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600444.1

ORF Insert Sequence:

ATGCCCTTCA GGAAAGCCTG TGGGCCGAAG CTGACCAACT CCCCAACGGT GATTGTGATG 
GTGGGGCTCC CAGCCCGGGG TAAGACTTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC 
TGGATAGGTG TTCCAACGAA AGTGTTCAAT GTGGGAGAAT ACCGACGGGA GGCTGTGAAG 
CAATACAGCT CTTACAACTT CTTCCGCCCT GACAATGAGG AGGCCATGAG AGTCCGAAAA 
CAGTGTGCCT TAGCTGCCCT GAGAGACGTC AAAAGCTACC TGACAAAGGA AGGAGGACAG 
ATTGCAGTTT TTGATGCCAC CAATACAACG AGAGAAAGGA GGCACATGAT CCTTCATTTT 
GCCAAAGAAA ATGACTTCAA GGCATTTTTC ATCGAGTCCG TCTGTGACGA CCCTACAGTT 
GTGGCCTCCA ATATCATGGA AGTGAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCC 
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAACTGCT ACGAAGCCAG CTACCAGCCT 
CTTGACCCTG ATAAATGTGA CAGGGACCTG TCATTCATCA AAGTGATTGA CGTTGGCCGG 
AGGTTCTTGG TGAACAGAGT GCAGGACCAC ATCCAGAGCC GAATCGTGTA TTACCTGATG 
AACATCCACG TGCAGCCCCG GACCATCTAC CTGTGTCGGC ACGGCGAGAA TGAGTACAAC 
GTGCAGGGCA AGATTGGGGG CGACTCAGGC CTGTCCAGCA GAGGCAAGAA GTTCGCCAAT 
GCCCTGAGCA AGTTCGTTGA AGAGCAGAAT CTGAAGGACC TCCGCGTGTG GACCAGTCAG 
CTGAAAAGTA CCATCCAGAC CGCGGAGGCC CTAAGGCTCC CCTACGAGCA GTGGAAGGCG 
CTCAATGAGA TTGATGCTGG TGTGTGTGAG GAACTAACCT ATGAGGAGAT CAGAGACACC 
TACCCTGAGG AGTATGCACT GCGTGAGCAA GACAAATACT ACTACCGCTA TCCCACAGGC 
GAGTCCTACC AGGATCTTGT CCAGCGCCTG GAGCCTGTGA TCATGGAATT AGAGCGCCAA 
GAGAACGTAT TGGTCATCTG TCACCAGGCT GTCCTGCGCT GCCTACTAGC CTACTTCCTT 
GACAAGAGTG CAGAGGAGAT GCCATACCTG AAATGCCCAC TTCACACAGT CCTGAAACTG 
ACGCCCGTGG CGTATGGGTG CCGTGTGGAG TCCATTTACT TGAATGTAGA ATCAGTGAGC 
ACGCATCGGG AGAGGTCAGA GGCTGTCAAA ATACAACACT TTGCCAGTGT AGTCAGACCT 
TCCTCTTACA CCGAACTTGA CTTTCTGTCT GTGGAGTCCG CAAAACAGGA TGCAAAGAAG 
GGACCTAACC CTCTCATGAG ACGCAATAGT GTCACCCCAC TAGCCAGCCC CGAGCCCACC 
AAAAAGCCTC GCATCAACAG CTTTGAGGAG CATGTGGCTT CCACTTCTGC TGCCCTGCCA 
AGCTGCCTGC CCCCGGAAGT GCCCACGCAG CTGCCCGGAC AACCTTTGCT AGGGAAAGCC 
TGTCTACGAA CTGTCTGTCA CATTTTTTCA AAGTTTTCTC CTTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of Akt/PKB and PFKFB isoenzymes in the control of glycolysis, cell proliferation and protein synthesis in mitogen-stimulated thymocytes.
Cellular signalling3423-37(2017 Jun)
Houddane A,Bultot L,Novellasdemunt L,Johanns M,Gueuning MA,Vertommen D,Coulie PG,Bartrons R,Hue L,Rider MH

PFKFB3-mediated glycolysis is involved in reactive astrocyte proliferation after oxygen-glucose deprivation/reperfusion and is regulated by Cdh1.
Neurochemistry international9126-33(2015 Dec)
Lv Y,Zhang B,Zhai C,Qiu J,Zhang Y,Yao W,Zhang C

Moderate grade hyperammonemia activates lactate dehydrogenase-4 and 6-phosphofructo-2-kinase to support increased lactate turnover in the brain slices.
Molecular and cellular biochemistry381(1-2)157-61(2013 Sep)
Mehrotra A,Trigun SK

Excitotoxic stimulus stabilizes PFKFB3 causing pentose-phosphate pathway to glycolysis switch and neurodegeneration.
Cell death and differentiation19(10)1582-9(2012 Oct)
Rodriguez-Rodriguez P,Fernandez E,Almeida A,Bola?os JP

Switches in 6-phosphofructo-2-kinase isoenzyme expression during rat sperm maturation.
Biochemical and biophysical research communications387(2)330-5(2009 Sep)
G?mez M,Navarro-Sabat? A,Manzano A,Duran J,Obach M,Bartrons R

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Pfkfb3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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