Hrk cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hrk
Entrez Gene ID	117271
Full Name	harakiri, BCL2 interacting protein
Synonyms	Bid3,Dp5
General protein information	Preferred Names harakiri, BCL2 interacting protein Names activator of apoptosis harakiri BH3 interacting (with BCL2 family) domain, apoptosis agonist BH3 interacting domain 3 BH3-interacting domain-containing protein 3 harakiri, BCL2 interacting protein (contains only BH3 domain) neuronal death protein DP5
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q16
Summary	member of the Bcl-2 family of proteins; has a role in programmed neuronal death [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_057130.4	NP_476471.1	activator of apoptosis harakiri

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Hrk	NP_476471.1
Mus musculus (house mouse)	Hrk	NP_031571.1

Gene Ontology
Bibliography

Related articles in PubMed

BH3 domains other than Bim and Bid can directly activate Bax/Bak.
Du H, Wolf J, Schafer B, Moldoveanu T, Chipuk JE, Kuwana T
The Journal of biological chemistry286(1)491-501(2011 Jan)

Signaling by IL-1beta+IFN-gamma and ER stress converge on DP5/Hrk activation: a novel mechanism for pancreatic beta-cell apoptosis.
Gurzov EN, Ortis F, Cunha DA, Gosset G, Li M, Cardozo AK, Eizirik DL
Cell death and differentiation16(11)1539-50(2009 Nov)

The proapoptotic dp5 gene is a direct target of the MLK-JNK-c-Jun pathway in sympathetic neurons.
Towers E, Gilley J, Randall R, Hughes R, Kristiansen M, Ham J
Nucleic acids research37(9)3044-60(2009 May)

BH3-only proteins in apoptosis and beyond: an overview.
Lomonosova E, Chinnadurai G
Oncogene27 Suppl 1S2-19(2008 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The inactivation of hrk protein protects -cells from IL-1beta+IFN-gamma and chemical Endoplasmic Reticulum stress-mediated cell death.
Title: Signaling by IL-1beta+IFN-gamma and ER stress converge on DP5/Hrk activation: a novel mechanism for pancreatic beta-cell apoptosis.

identification of a conserved ATF site in the dp5 promoter that binds c-Jun and ATF2, which is critical for dp5 promoter induction after NGF withdrawal.
Title: The proapoptotic dp5 gene is a direct target of the MLK-JNK-c-Jun pathway in sympathetic neurons.

Bcl-2 regulates an essential pool of mitochondrial GSH and this regulation may depend upon Bcl-2 directly interacting with GSH via the BH3 groove
Title: Glutathione binding to the Bcl-2 homology-3 domain groove: a molecular basis for Bcl-2 antioxidant function at mitochondria.

dp5 is a target gene of c-Jun and plays a critical role in potassium deprivation-induced apoptosis in cerebellar granule neurons.
Title: dp5/HRK is a c-Jun target gene and required for apoptosis induced by potassium deprivation in cerebellar granule neurons.

These results suggest that JNK activation contributes to trauma-induced DP5 expression and subsequent apoptosis in SCI.
Title: JNK activation contributes to DP5 induction and apoptosis following traumatic spinal cord injury.

The following Hrk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hrk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13731	NM_057130.4 Latest version!	Rattus norvegicus harakiri, BCL2 interacting protein (Hrk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hrk ( NM_057130.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_476471.1 Rattus norvegicus harakiri, BCL2 interacting protein (Hrk), mRNA.

Price & Availability↑

CloneID	ORa13731
Clone ID Related Accession (Same CDS sequence)	NM_057130.4
Accession Version	NM_057130.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	activator of apoptosis harakiri
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from D83697.1. On Sep 17, 2014 this sequence version replaced NM_057130.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D83697.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGCCCGT GTCCCCGGCA TCGCGGCCGC GGGCCCCCGG CCGTGTGCGG TTGCGGCGAC 
GCTCGCCCCG GGCTGCGCTG GGCGGCGGCG CAGGTGACCG CGCTGCGGCT GCAGGCGCTG 
GGCGACGAGC TGCACCGACG CGCCATGAGG CGTCGCGCGC GGCCCCGGGA CCCGCTGCCC 
GCGCTGCTGC CCGCGCTCCG CGCCCGCTGG CCCTGGCTGT GCGCGGCCGC GCAGGTGGCG 
GCGCTGGCGG CCTGGCTGCT CGGCAGGCGG AGCGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476471.1
CDS	26..304
Translation	MCPCPRHRGRGPPAVCGCGDARPGLRWAAAQVTALRLQALGDELHRRAMRRRARPRDPLPALLPALRARWPWLCAAAQVAALAAWLLGRRSA

Target ORF information:

RefSeq Version	NM_057130.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus harakiri, BCL2 interacting protein (Hrk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057130.4

ORF Insert Sequence:

ATGTGCCCGT GTCCCCGGCA TCGCGGCCGC GGGCCCCCGG CCGTGTGCGG TTGCGGCGAC 
GCTCGCCCCG GGCTGCGCTG GGCGGCGGCG CAGGTGACCG CGCTGCGGCT GCAGGCGCTG 
GGCGACGAGC TGCACCGACG CGCCATGAGG CGTCGCGCGC GGCCCCGGGA CCCGCTGCCC 
GCGCTGCTGC CCGCGCTCCG CGCCCGCTGG CCCTGGCTGT GCGCGGCCGC GCAGGTGGCG 
GCGCTGGCGG CCTGGCTGCT CGGCAGGCGG AGCGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

BH3 domains other than Bim and Bid can directly activate Bax/Bak.
The Journal of biological chemistry286(1)491-501(2011 Jan)
Du H,Wolf J,Schafer B,Moldoveanu T,Chipuk JE,Kuwana T

Signaling by IL-1beta+IFN-gamma and ER stress converge on DP5/Hrk activation: a novel mechanism for pancreatic beta-cell apoptosis.
Cell death and differentiation16(11)1539-50(2009 Nov)
Gurzov EN,Ortis F,Cunha DA,Gosset G,Li M,Cardozo AK,Eizirik DL

The proapoptotic dp5 gene is a direct target of the MLK-JNK-c-Jun pathway in sympathetic neurons.
Nucleic acids research37(9)3044-60(2009 May)
Towers E,Gilley J,Randall R,Hughes R,Kristiansen M,Ham J

BH3-only proteins in apoptosis and beyond: an overview.
Oncogene27 Suppl 1S2-19(2008 Dec)
Lomonosova E,Chinnadurai G

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Hrk cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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