Gpr176 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gpr176
Entrez Gene ID	117257
Full Name	G protein-coupled receptor 176
Synonyms	Gm1012,Gpr,RBU-15
General protein information	Preferred Names G protein-coupled receptor 176 Names probable G-protein coupled receptor 176 G-protein coupled receptor AGR9 putative G protein coupled receptor
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q35
Summary	orphan G protein coupled receptor found in brain and spleen [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017591435.1	XP_017446924.1	probable G-protein coupled receptor 176 isoform X2
XM_017591436.1	XP_017446925.1	probable G-protein coupled receptor 176 isoform X2
NM_001270986.1	NP_001257915.1	probable G-protein coupled receptor 176
XM_017591434.1	XP_017446923.1	probable G-protein coupled receptor 176 isoform X1
XM_017591437.1	XP_017446926.1	probable G-protein coupled receptor 176 isoform X2

Pathways from BioSystems

Homologies

	GPR176	XP_001092703.1
Homo sapiens (human)	GPR176	NP_009154.1
Xenopus tropicalis (tropical clawed frog)	gpr176	NP_001096334.1
Pan troglodytes (chimpanzee)	GPR176	XP_003314653.1
Canis lupus familiaris (dog)	GPR176	XP_544612.2
Danio rerio (zebrafish)	gpr176	NP_001122183.1
Bos taurus (cattle)	GPR176	NP_001179545.1
Mus musculus (house mouse)	Gpr176	NP_958755.1
Rattus norvegicus (Norway rat)	Gpr176	NP_001257915.1
Gallus gallus (chicken)	GPR176	XP_004941780.1

Gene Ontology
Bibliography

Related articles in PubMed

Gpr176 is a Gz-linked orphan G-protein-coupled receptor that sets the pace of circadian behaviour.
Doi M, Murai I, Kunisue S, Setsu G, Uchio N, Tanaka R, Kobayashi S, Shimatani H, Hayashi H, Chao HW, Nakagawa Y, Takahashi Y, Hotta Y, Yasunaga J, Matsuoka M, Hastings MH, Kiyonari H, Okamura H
Nature communications710583(2016 Feb)

cDNA cloning of a putative G protein-coupled receptor from brain.
Hata S, Emi Y, Iyanagi T, Osumi T
Biochimica et biophysica acta1261(1)121-5(1995 Mar)

Molecular cloning of a novel putative G protein-coupled receptor from rat aortic smooth muscle. Downregulation of the mRNA level by the cyclic AMP messenger pathway.
Ishizaka N, Okazaki H, Kurokawa K, Kumada M, Takuwa Y
Biochimica et biophysica acta1218(2)173-80(1994 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gpr176 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gpr176 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa10035	NM_001270986.1 Latest version!	Rattus norvegicus G protein-coupled receptor 176 (Gpr176), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa60241	XM_017591434.1 Latest version!	Rattus norvegicus G protein-coupled receptor 176 (Gpr176), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa60242	XM_017591437.1 Latest version!	Rattus norvegicus G protein-coupled receptor 176 (Gpr176), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa60242	XM_017591436.1 Latest version!	Rattus norvegicus G protein-coupled receptor 176 (Gpr176), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa60242	XM_017591435.1 Latest version!	Rattus norvegicus G protein-coupled receptor 176 (Gpr176), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gpr176 ( NM_001270986.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001257915.1 Rattus norvegicus G protein-coupled receptor 176 (Gpr176), mRNA.

Price & Availability↑

CloneID	ORa10035
Clone ID Related Accession (Same CDS sequence)	NM_001270986.1
Accession Version	NM_001270986.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rattus norvegicus(Norway rat)
Product	probable G-protein coupled receptor 176
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07053494.1 and AABR07053493.1. On Aug 18, 2012 this sequence version replaced XM_342493.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: FQ144991.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGGCACA ACGGCAGCTG GGTGTCACCG AACACCAGCC ACCCGCGTAA CACGTCGGGC 
GCCCAGGCTG GCGCCAACAG CAGCGCGTTC GGGGAGCTGA GCGAGGCGCA GCTGTACCGC 
CAGTTCACCA CCACCGTGCA GGTCGTCATC TTCATTGGTT CGCTGCTCGG GAACTTCACG 
GTGTTATGGT CAACCTGCCG CACGACCGTG TTCAAATCTG TCACCAACAG GTTCATTAAA 
AACCTGGCCT GCTCCGGGAT TTGTGCCAGC GTGGTCTGCG TGCCCTTCGA CATCATCCTG 
AGCACCAGCC CTCACTGCTG CTGGTGGATC TACACGATGC TCTTCTGCAA GGTGCTTAAA 
TTCCTACACA AGGTGTTCTG CTCGGTGACT GTCCTCAGCT TTCCTGCCAT TGCCCTGGAC 
AGGTACTATT CAGTCCTCTA CCCTCTGGAG AGAAAAATCT CTGACGCCAA GTCTCGGGAA 
CTAGTGATGT ACATCTGGGC CCATGCAGTG GTGGCCAGCG TCCCTGTGTT TGCTGTGACC 
AATGTAGCTG ACATCTATGC TACATCCACC TGCACGGAAG TCTGGAGCAA CTCCTTGGGC 
CACCTGGTGT ATGTCCTGAT CTACAACGTC ACCACGGTCA TTGTGCCTGT GGCTGTGGTA 
TTCCTCTTTC TTATCCTGAT CCGGAGGGCC CTGAGTGCCA GTCAGAAGAA GAAAGTCATC 
ATCGCTGCAC TGAGGACCCC ACAGAACACC ATCTCTATCC CCTATGCATC CCAGCGGGAG 
GCAGAGCTAC ATGCCACCCT GCTCTCCATG GTGACAGTTT TCATCCTCTG CAGCGTACCC 
TATGCCACCC TGGTTGTCTA CCAGACTGTG CTCAATGTAC CTAACACTTC TGTGTTCTTG 
CTTCTCACTG CTATTTGGCT GCCCAAAGTC TCACTGTTGG CCAACCCTGT GCTCTTCCTA 
ACTGTGAACA GATCAGTACG CAAATGCTTG GTGGGGACCC TGGTGCAGTT GCACCACCGG 
TACAGTCGCC GCAATGTGGT TAGTACAGGG AGTGGGGTGG TTGAGCCTAG CCTTGAGCCC 
AGCATGCGCT CTGGTAGCCA GCTCTTGGAG ATGTTCCACA TTGGACAGCA ACAGATCTTT 
AAGCCCTCGG AGGATGAGGA AGAGAGTGAG GCCAAGTATC TTGGCTCAAC TGATTTCCAG 
GCCAAGGAGG TACTCACCTC CTCCCCAGAG GGCGAGGAGT CACAGCTTGC ACCCTCTGTG 
CCGCCTCCAG GCACAGTAGA CTCTGTATCT CGGGTGTCCC CAGTAGCCCC CGTGGAACCT 
GGCATATTCC CTGATAAGTA TTCCCTGCAG TTTGGCTTTG GGCCTTTCGA GTTGCCCCCT 
CAGTGGCTCT CTGAGACTCG AAATAGCAAG AAGAGGCTGC TTCCCCCACT GGGCAATACC 
CCAGAAGAGC TGATCCAGAC AAAGGTGCCC AGAGTGAGCA GGGTGGAGCG GAAGATGAGC 
AGAAACAATA AAGTGAGCAT TTTTCCAAAG GTAGACTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257915.1
CDS	186..1727
Translation	MGHNGSWVSPNTSHPRNTSGAQAGANSSAFGELSEAQLYRQFTTTVQVVIFIGSLLGNFTVLWSTCRTTVFKSVTNRFIKNLACSGICASVVCVPFDIILSTSPHCCWWIYTMLFCKVLKFLHKVFCSVTVLSFPAIALDRYYSVLYPLERKISDAKSRELVMYIWAHAVVASVPVFAVTNVADIYATSTCTEVWSNSLGHLVYVLIYNVTTVIVPVAVVFLFLILIRRALSASQKKKVIIAALRTPQNTISIPYASQREAELHATLLSMVTVFILCSVPYATLVVYQTVLNVPNTSVFLLLTAIWLPKVSLLANPVLFLTVNRSVRKCLVGTLVQLHHRYSRRNVVSTGSGVVEPSLEPSMRSGSQLLEMFHIGQQQIFKPSEDEEESEAKYLGSTDFQAKEVLTSSPEGEESQLAPSVPPPGTVDSVSRVSPVAPVEPGIFPDKYSLQFGFGPFELPPQWLSETRNSKKRLLPPLGNTPEELIQTKVPRVSRVERKMSRNNKVSIFPKVDS

Target ORF information:

RefSeq Version	NM_001270986.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G protein-coupled receptor 176 (Gpr176), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270986.1

ORF Insert Sequence:

ATGGGGCACA ACGGCAGCTG GGTGTCACCG AACACCAGCC ACCCGCGTAA CACGTCGGGC 
GCCCAGGCTG GCGCCAACAG CAGCGCGTTC GGGGAGCTGA GCGAGGCGCA GCTGTACCGC 
CAGTTCACCA CCACCGTGCA GGTCGTCATC TTCATTGGTT CGCTGCTCGG GAACTTCACG 
GTGTTATGGT CAACCTGCCG CACGACCGTG TTCAAATCTG TCACCAACAG GTTCATTAAA 
AACCTGGCCT GCTCCGGGAT TTGTGCCAGC GTGGTCTGCG TGCCCTTCGA CATCATCCTG 
AGCACCAGCC CTCACTGCTG CTGGTGGATC TACACGATGC TCTTCTGCAA GGTGCTTAAA 
TTCCTACACA AGGTGTTCTG CTCGGTGACT GTCCTCAGCT TTCCTGCCAT TGCCCTGGAC 
AGGTACTATT CAGTCCTCTA CCCTCTGGAG AGAAAAATCT CTGACGCCAA GTCTCGGGAA 
CTAGTGATGT ACATCTGGGC CCATGCAGTG GTGGCCAGCG TCCCTGTGTT TGCTGTGACC 
AATGTAGCTG ACATCTATGC TACATCCACC TGCACGGAAG TCTGGAGCAA CTCCTTGGGC 
CACCTGGTGT ATGTCCTGAT CTACAACGTC ACCACGGTCA TTGTGCCTGT GGCTGTGGTA 
TTCCTCTTTC TTATCCTGAT CCGGAGGGCC CTGAGTGCCA GTCAGAAGAA GAAAGTCATC 
ATCGCTGCAC TGAGGACCCC ACAGAACACC ATCTCTATCC CCTATGCATC CCAGCGGGAG 
GCAGAGCTAC ATGCCACCCT GCTCTCCATG GTGACAGTTT TCATCCTCTG CAGCGTACCC 
TATGCCACCC TGGTTGTCTA CCAGACTGTG CTCAATGTAC CTAACACTTC TGTGTTCTTG 
CTTCTCACTG CTATTTGGCT GCCCAAAGTC TCACTGTTGG CCAACCCTGT GCTCTTCCTA 
ACTGTGAACA GATCAGTACG CAAATGCTTG GTGGGGACCC TGGTGCAGTT GCACCACCGG 
TACAGTCGCC GCAATGTGGT TAGTACAGGG AGTGGGGTGG TTGAGCCTAG CCTTGAGCCC 
AGCATGCGCT CTGGTAGCCA GCTCTTGGAG ATGTTCCACA TTGGACAGCA ACAGATCTTT 
AAGCCCTCGG AGGATGAGGA AGAGAGTGAG GCCAAGTATC TTGGCTCAAC TGATTTCCAG 
GCCAAGGAGG TACTCACCTC CTCCCCAGAG GGCGAGGAGT CACAGCTTGC ACCCTCTGTG 
CCGCCTCCAG GCACAGTAGA CTCTGTATCT CGGGTGTCCC CAGTAGCCCC CGTGGAACCT 
GGCATATTCC CTGATAAGTA TTCCCTGCAG TTTGGCTTTG GGCCTTTCGA GTTGCCCCCT 
CAGTGGCTCT CTGAGACTCG AAATAGCAAG AAGAGGCTGC TTCCCCCACT GGGCAATACC 
CCAGAAGAGC TGATCCAGAC AAAGGTGCCC AGAGTGAGCA GGGTGGAGCG GAAGATGAGC 
AGAAACAATA AAGTGAGCAT TTTTCCAAAG GTAGACTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpr176 ( XM_017591434.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446923.1 Rattus norvegicus G protein-coupled receptor 176 (Gpr176), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa60241
Clone ID Related Accession (Same CDS sequence)	XM_017591434.1
Accession Version	XM_017591434.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	probable G-protein coupled receptor 176 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATT CTGTACCGTC TGGAGTCACC TTGCCCTCTG GCGGTGCTAA GCTCCTTGCT 
GTTAATGAAC TTGTGCTGTA TGTGACCTTC CTGCACGGTG GTGGAGCTCG CCAATCCTGC 
CCCTCAGGGA ACTTCACGGT GTTATGGTCA ACCTGCCGCA CGACCGTGTT CAAATCTGTC 
ACCAACAGGT TCATTAAAAA CCTGGCCTGC TCCGGGATTT GTGCCAGCGT GGTCTGCGTG 
CCCTTCGACA TCATCCTGAG CACCAGCCCT CACTGCTGCT GGTGGATCTA CACGATGCTC 
TTCTGCAAGG TGCTTAAATT CCTACACAAG GTGTTCTGCT CGGTGACTGT CCTCAGCTTT 
CCTGCCATTG CCCTGGACAG GTACTATTCA GTCCTCTACC CTCTGGAGAG AAAAATCTCT 
GACGCCAAGT CTCGGGAACT AGTGATGTAC ATCTGGGCCC ATGCAGTGGT GGCCAGCGTC 
CCTGTGTTTG CTGTGACCAA TGTAGCTGAC ATCTATGCTA CATCCACCTG CACGGAAGTC 
TGGAGCAACT CCTTGGGCCA CCTGGTGTAT GTCCTGATCT ACAACGTCAC CACGGTCATT 
GTGCCTGTGG CTGTGGTATT CCTCTTTCTT ATCCTGATCC GGAGGGCCCT GAGTGCCAGT 
CAGAAGAAGA AAGTCATCAT CGCTGCACTG AGGACCCCAC AGAACACCAT CTCTATCCCC 
TATGCATCCC AGCGGGAGGC AGAGCTACAT GCCACCCTGC TCTCCATGGT GACAGTTTTC 
ATCCTCTGCA GCGTACCCTA TGCCACCCTG GTTGTCTACC AGACTGTGCT CAATGTACCT 
AACACTTCTG TGTTCTTGCT TCTCACTGCT ATTTGGCTGC CCAAAGTCTC ACTGTTGGCC 
AACCCTGTGC TCTTCCTAAC TGTGAACAGA TCAGTACGCA AATGCTTGGT GGGGACCCTG 
GTGCAGTTGC ACCACCGGTA CAGTCGCCGC AATGTGGTTA GTACAGGGAG TGGGGTGGTT 
GAGCCTAGCC TTGAGCCCAG CATGCGCTCT GGTAGCCAGC TCTTGGAGAT GTTCCACATT 
GGACAGCAAC AGATCTTTAA GCCCTCGGAG GATGAGGAAG AGAGTGAGGC CAAGTATCTT 
GGCTCAACTG ATTTCCAGGC CAAGGAGGTA CTCACCTCCT CCCCAGAGGG CGAGGAGTCA 
CAGCTTGCAC CCTCTGTGCC GCCTCCAGGC ACAGTAGACT CTGTATCTCG GGTGTCCCCA 
GTAGCCCCCG TGGAACCTGG CATATTCCCT GATAAGTATT CCCTGCAGTT TGGCTTTGGG 
CCTTTCGAGT TGCCCCCTCA GTGGCTCTCT GAGACTCGAA ATAGCAAGAA GAGGCTGCTT 
CCCCCACTGG GCAATACCCC AGAAGAGCTG ATCCAGACAA AGGTGCCCAG AGTGAGCAGG 
GTGGAGCGGA AGATGAGCAG AAACAATAAA GTGAGCATTT TTCCAAAGGT AGACTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446923.1
CDS	224..1723
Translation	MDDSVPSGVTLPSGGAKLLAVNELVLYVTFLHGGGARQSCPSGNFTVLWSTCRTTVFKSVTNRFIKNLACSGICASVVCVPFDIILSTSPHCCWWIYTMLFCKVLKFLHKVFCSVTVLSFPAIALDRYYSVLYPLERKISDAKSRELVMYIWAHAVVASVPVFAVTNVADIYATSTCTEVWSNSLGHLVYVLIYNVTTVIVPVAVVFLFLILIRRALSASQKKKVIIAALRTPQNTISIPYASQREAELHATLLSMVTVFILCSVPYATLVVYQTVLNVPNTSVFLLLTAIWLPKVSLLANPVLFLTVNRSVRKCLVGTLVQLHHRYSRRNVVSTGSGVVEPSLEPSMRSGSQLLEMFHIGQQQIFKPSEDEEESEAKYLGSTDFQAKEVLTSSPEGEESQLAPSVPPPGTVDSVSRVSPVAPVEPGIFPDKYSLQFGFGPFELPPQWLSETRNSKKRLLPPLGNTPEELIQTKVPRVSRVERKMSRNNKVSIFPKVDS

Target ORF information:

RefSeq Version	XM_017591434.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G protein-coupled receptor 176 (Gpr176), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591434.1

ORF Insert Sequence:

ATGGATGATT CTGTACCGTC TGGAGTCACC TTGCCCTCTG GCGGTGCTAA GCTCCTTGCT 
GTTAATGAAC TTGTGCTGTA TGTGACCTTC CTGCACGGTG GTGGAGCTCG CCAATCCTGC 
CCCTCAGGGA ACTTCACGGT GTTATGGTCA ACCTGCCGCA CGACCGTGTT CAAATCTGTC 
ACCAACAGGT TCATTAAAAA CCTGGCCTGC TCCGGGATTT GTGCCAGCGT GGTCTGCGTG 
CCCTTCGACA TCATCCTGAG CACCAGCCCT CACTGCTGCT GGTGGATCTA CACGATGCTC 
TTCTGCAAGG TGCTTAAATT CCTACACAAG GTGTTCTGCT CGGTGACTGT CCTCAGCTTT 
CCTGCCATTG CCCTGGACAG GTACTATTCA GTCCTCTACC CTCTGGAGAG AAAAATCTCT 
GACGCCAAGT CTCGGGAACT AGTGATGTAC ATCTGGGCCC ATGCAGTGGT GGCCAGCGTC 
CCTGTGTTTG CTGTGACCAA TGTAGCTGAC ATCTATGCTA CATCCACCTG CACGGAAGTC 
TGGAGCAACT CCTTGGGCCA CCTGGTGTAT GTCCTGATCT ACAACGTCAC CACGGTCATT 
GTGCCTGTGG CTGTGGTATT CCTCTTTCTT ATCCTGATCC GGAGGGCCCT GAGTGCCAGT 
CAGAAGAAGA AAGTCATCAT CGCTGCACTG AGGACCCCAC AGAACACCAT CTCTATCCCC 
TATGCATCCC AGCGGGAGGC AGAGCTACAT GCCACCCTGC TCTCCATGGT GACAGTTTTC 
ATCCTCTGCA GCGTACCCTA TGCCACCCTG GTTGTCTACC AGACTGTGCT CAATGTACCT 
AACACTTCTG TGTTCTTGCT TCTCACTGCT ATTTGGCTGC CCAAAGTCTC ACTGTTGGCC 
AACCCTGTGC TCTTCCTAAC TGTGAACAGA TCAGTACGCA AATGCTTGGT GGGGACCCTG 
GTGCAGTTGC ACCACCGGTA CAGTCGCCGC AATGTGGTTA GTACAGGGAG TGGGGTGGTT 
GAGCCTAGCC TTGAGCCCAG CATGCGCTCT GGTAGCCAGC TCTTGGAGAT GTTCCACATT 
GGACAGCAAC AGATCTTTAA GCCCTCGGAG GATGAGGAAG AGAGTGAGGC CAAGTATCTT 
GGCTCAACTG ATTTCCAGGC CAAGGAGGTA CTCACCTCCT CCCCAGAGGG CGAGGAGTCA 
CAGCTTGCAC CCTCTGTGCC GCCTCCAGGC ACAGTAGACT CTGTATCTCG GGTGTCCCCA 
GTAGCCCCCG TGGAACCTGG CATATTCCCT GATAAGTATT CCCTGCAGTT TGGCTTTGGG 
CCTTTCGAGT TGCCCCCTCA GTGGCTCTCT GAGACTCGAA ATAGCAAGAA GAGGCTGCTT 
CCCCCACTGG GCAATACCCC AGAAGAGCTG ATCCAGACAA AGGTGCCCAG AGTGAGCAGG 
GTGGAGCGGA AGATGAGCAG AAACAATAAA GTGAGCATTT TTCCAAAGGT AGACTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpr176 ( XM_017591435.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446924.1 Rattus norvegicus G protein-coupled receptor 176 (Gpr176), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa60242
Clone ID Related Accession (Same CDS sequence)	XM_017591435.1 , XM_017591436.1 , XM_017591437.1
Accession Version	XM_017591435.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	probable G-protein coupled receptor 176 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGGA ACTTCACGGT GTTATGGTCA ACCTGCCGCA CGACCGTGTT CAAATCTGTC 
ACCAACAGGT TCATTAAAAA CCTGGCCTGC TCCGGGATTT GTGCCAGCGT GGTCTGCGTG 
CCCTTCGACA TCATCCTGAG CACCAGCCCT CACTGCTGCT GGTGGATCTA CACGATGCTC 
TTCTGCAAGG TGCTTAAATT CCTACACAAG GTGTTCTGCT CGGTGACTGT CCTCAGCTTT 
CCTGCCATTG CCCTGGACAG GTACTATTCA GTCCTCTACC CTCTGGAGAG AAAAATCTCT 
GACGCCAAGT CTCGGGAACT AGTGATGTAC ATCTGGGCCC ATGCAGTGGT GGCCAGCGTC 
CCTGTGTTTG CTGTGACCAA TGTAGCTGAC ATCTATGCTA CATCCACCTG CACGGAAGTC 
TGGAGCAACT CCTTGGGCCA CCTGGTGTAT GTCCTGATCT ACAACGTCAC CACGGTCATT 
GTGCCTGTGG CTGTGGTATT CCTCTTTCTT ATCCTGATCC GGAGGGCCCT GAGTGCCAGT 
CAGAAGAAGA AAGTCATCAT CGCTGCACTG AGGACCCCAC AGAACACCAT CTCTATCCCC 
TATGCATCCC AGCGGGAGGC AGAGCTACAT GCCACCCTGC TCTCCATGGT GACAGTTTTC 
ATCCTCTGCA GCGTACCCTA TGCCACCCTG GTTGTCTACC AGACTGTGCT CAATGTACCT 
AACACTTCTG TGTTCTTGCT TCTCACTGCT ATTTGGCTGC CCAAAGTCTC ACTGTTGGCC 
AACCCTGTGC TCTTCCTAAC TGTGAACAGA TCAGTACGCA AATGCTTGGT GGGGACCCTG 
GTGCAGTTGC ACCACCGGTA CAGTCGCCGC AATGTGGTTA GTACAGGGAG TGGGGTGGTT 
GAGCCTAGCC TTGAGCCCAG CATGCGCTCT GGTAGCCAGC TCTTGGAGAT GTTCCACATT 
GGACAGCAAC AGATCTTTAA GCCCTCGGAG GATGAGGAAG AGAGTGAGGC CAAGTATCTT 
GGCTCAACTG ATTTCCAGGC CAAGGAGGTA CTCACCTCCT CCCCAGAGGG CGAGGAGTCA 
CAGCTTGCAC CCTCTGTGCC GCCTCCAGGC ACAGTAGACT CTGTATCTCG GGTGTCCCCA 
GTAGCCCCCG TGGAACCTGG CATATTCCCT GATAAGTATT CCCTGCAGTT TGGCTTTGGG 
CCTTTCGAGT TGCCCCCTCA GTGGCTCTCT GAGACTCGAA ATAGCAAGAA GAGGCTGCTT 
CCCCCACTGG GCAATACCCC AGAAGAGCTG ATCCAGACAA AGGTGCCCAG AGTGAGCAGG 
GTGGAGCGGA AGATGAGCAG AAACAATAAA GTGAGCATTT TTCCAAAGGT AGACTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446924.1
CDS	1731..3110
Translation	MFGNFTVLWSTCRTTVFKSVTNRFIKNLACSGICASVVCVPFDIILSTSPHCCWWIYTMLFCKVLKFLHKVFCSVTVLSFPAIALDRYYSVLYPLERKISDAKSRELVMYIWAHAVVASVPVFAVTNVADIYATSTCTEVWSNSLGHLVYVLIYNVTTVIVPVAVVFLFLILIRRALSASQKKKVIIAALRTPQNTISIPYASQREAELHATLLSMVTVFILCSVPYATLVVYQTVLNVPNTSVFLLLTAIWLPKVSLLANPVLFLTVNRSVRKCLVGTLVQLHHRYSRRNVVSTGSGVVEPSLEPSMRSGSQLLEMFHIGQQQIFKPSEDEEESEAKYLGSTDFQAKEVLTSSPEGEESQLAPSVPPPGTVDSVSRVSPVAPVEPGIFPDKYSLQFGFGPFELPPQWLSETRNSKKRLLPPLGNTPEELIQTKVPRVSRVERKMSRNNKVSIFPKVDS

Target ORF information:

RefSeq Version	XM_017591435.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G protein-coupled receptor 176 (Gpr176), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591435.1

ORF Insert Sequence:

ATGTTTGGGA ACTTCACGGT GTTATGGTCA ACCTGCCGCA CGACCGTGTT CAAATCTGTC 
ACCAACAGGT TCATTAAAAA CCTGGCCTGC TCCGGGATTT GTGCCAGCGT GGTCTGCGTG 
CCCTTCGACA TCATCCTGAG CACCAGCCCT CACTGCTGCT GGTGGATCTA CACGATGCTC 
TTCTGCAAGG TGCTTAAATT CCTACACAAG GTGTTCTGCT CGGTGACTGT CCTCAGCTTT 
CCTGCCATTG CCCTGGACAG GTACTATTCA GTCCTCTACC CTCTGGAGAG AAAAATCTCT 
GACGCCAAGT CTCGGGAACT AGTGATGTAC ATCTGGGCCC ATGCAGTGGT GGCCAGCGTC 
CCTGTGTTTG CTGTGACCAA TGTAGCTGAC ATCTATGCTA CATCCACCTG CACGGAAGTC 
TGGAGCAACT CCTTGGGCCA CCTGGTGTAT GTCCTGATCT ACAACGTCAC CACGGTCATT 
GTGCCTGTGG CTGTGGTATT CCTCTTTCTT ATCCTGATCC GGAGGGCCCT GAGTGCCAGT 
CAGAAGAAGA AAGTCATCAT CGCTGCACTG AGGACCCCAC AGAACACCAT CTCTATCCCC 
TATGCATCCC AGCGGGAGGC AGAGCTACAT GCCACCCTGC TCTCCATGGT GACAGTTTTC 
ATCCTCTGCA GCGTACCCTA TGCCACCCTG GTTGTCTACC AGACTGTGCT CAATGTACCT 
AACACTTCTG TGTTCTTGCT TCTCACTGCT ATTTGGCTGC CCAAAGTCTC ACTGTTGGCC 
AACCCTGTGC TCTTCCTAAC TGTGAACAGA TCAGTACGCA AATGCTTGGT GGGGACCCTG 
GTGCAGTTGC ACCACCGGTA CAGTCGCCGC AATGTGGTTA GTACAGGGAG TGGGGTGGTT 
GAGCCTAGCC TTGAGCCCAG CATGCGCTCT GGTAGCCAGC TCTTGGAGAT GTTCCACATT 
GGACAGCAAC AGATCTTTAA GCCCTCGGAG GATGAGGAAG AGAGTGAGGC CAAGTATCTT 
GGCTCAACTG ATTTCCAGGC CAAGGAGGTA CTCACCTCCT CCCCAGAGGG CGAGGAGTCA 
CAGCTTGCAC CCTCTGTGCC GCCTCCAGGC ACAGTAGACT CTGTATCTCG GGTGTCCCCA 
GTAGCCCCCG TGGAACCTGG CATATTCCCT GATAAGTATT CCCTGCAGTT TGGCTTTGGG 
CCTTTCGAGT TGCCCCCTCA GTGGCTCTCT GAGACTCGAA ATAGCAAGAA GAGGCTGCTT 
CCCCCACTGG GCAATACCCC AGAAGAGCTG ATCCAGACAA AGGTGCCCAG AGTGAGCAGG 
GTGGAGCGGA AGATGAGCAG AAACAATAAA GTGAGCATTT TTCCAAAGGT AGACTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpr176 ( XM_017591436.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446925.1 Rattus norvegicus G protein-coupled receptor 176 (Gpr176), transcript variant X3, mRNA.

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CloneID	ORa60242
Clone ID Related Accession (Same CDS sequence)	XM_017591435.1 , XM_017591436.1 , XM_017591437.1
Accession Version	XM_017591436.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	probable G-protein coupled receptor 176 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGGA ACTTCACGGT GTTATGGTCA ACCTGCCGCA CGACCGTGTT CAAATCTGTC 
ACCAACAGGT TCATTAAAAA CCTGGCCTGC TCCGGGATTT GTGCCAGCGT GGTCTGCGTG 
CCCTTCGACA TCATCCTGAG CACCAGCCCT CACTGCTGCT GGTGGATCTA CACGATGCTC 
TTCTGCAAGG TGCTTAAATT CCTACACAAG GTGTTCTGCT CGGTGACTGT CCTCAGCTTT 
CCTGCCATTG CCCTGGACAG GTACTATTCA GTCCTCTACC CTCTGGAGAG AAAAATCTCT 
GACGCCAAGT CTCGGGAACT AGTGATGTAC ATCTGGGCCC ATGCAGTGGT GGCCAGCGTC 
CCTGTGTTTG CTGTGACCAA TGTAGCTGAC ATCTATGCTA CATCCACCTG CACGGAAGTC 
TGGAGCAACT CCTTGGGCCA CCTGGTGTAT GTCCTGATCT ACAACGTCAC CACGGTCATT 
GTGCCTGTGG CTGTGGTATT CCTCTTTCTT ATCCTGATCC GGAGGGCCCT GAGTGCCAGT 
CAGAAGAAGA AAGTCATCAT CGCTGCACTG AGGACCCCAC AGAACACCAT CTCTATCCCC 
TATGCATCCC AGCGGGAGGC AGAGCTACAT GCCACCCTGC TCTCCATGGT GACAGTTTTC 
ATCCTCTGCA GCGTACCCTA TGCCACCCTG GTTGTCTACC AGACTGTGCT CAATGTACCT 
AACACTTCTG TGTTCTTGCT TCTCACTGCT ATTTGGCTGC CCAAAGTCTC ACTGTTGGCC 
AACCCTGTGC TCTTCCTAAC TGTGAACAGA TCAGTACGCA AATGCTTGGT GGGGACCCTG 
GTGCAGTTGC ACCACCGGTA CAGTCGCCGC AATGTGGTTA GTACAGGGAG TGGGGTGGTT 
GAGCCTAGCC TTGAGCCCAG CATGCGCTCT GGTAGCCAGC TCTTGGAGAT GTTCCACATT 
GGACAGCAAC AGATCTTTAA GCCCTCGGAG GATGAGGAAG AGAGTGAGGC CAAGTATCTT 
GGCTCAACTG ATTTCCAGGC CAAGGAGGTA CTCACCTCCT CCCCAGAGGG CGAGGAGTCA 
CAGCTTGCAC CCTCTGTGCC GCCTCCAGGC ACAGTAGACT CTGTATCTCG GGTGTCCCCA 
GTAGCCCCCG TGGAACCTGG CATATTCCCT GATAAGTATT CCCTGCAGTT TGGCTTTGGG 
CCTTTCGAGT TGCCCCCTCA GTGGCTCTCT GAGACTCGAA ATAGCAAGAA GAGGCTGCTT 
CCCCCACTGG GCAATACCCC AGAAGAGCTG ATCCAGACAA AGGTGCCCAG AGTGAGCAGG 
GTGGAGCGGA AGATGAGCAG AAACAATAAA GTGAGCATTT TTCCAAAGGT AGACTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446925.1
CDS	1706..3085
Translation	MFGNFTVLWSTCRTTVFKSVTNRFIKNLACSGICASVVCVPFDIILSTSPHCCWWIYTMLFCKVLKFLHKVFCSVTVLSFPAIALDRYYSVLYPLERKISDAKSRELVMYIWAHAVVASVPVFAVTNVADIYATSTCTEVWSNSLGHLVYVLIYNVTTVIVPVAVVFLFLILIRRALSASQKKKVIIAALRTPQNTISIPYASQREAELHATLLSMVTVFILCSVPYATLVVYQTVLNVPNTSVFLLLTAIWLPKVSLLANPVLFLTVNRSVRKCLVGTLVQLHHRYSRRNVVSTGSGVVEPSLEPSMRSGSQLLEMFHIGQQQIFKPSEDEEESEAKYLGSTDFQAKEVLTSSPEGEESQLAPSVPPPGTVDSVSRVSPVAPVEPGIFPDKYSLQFGFGPFELPPQWLSETRNSKKRLLPPLGNTPEELIQTKVPRVSRVERKMSRNNKVSIFPKVDS

Target ORF information:

RefSeq Version	XM_017591436.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G protein-coupled receptor 176 (Gpr176), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591436.1

ORF Insert Sequence:

ATGTTTGGGA ACTTCACGGT GTTATGGTCA ACCTGCCGCA CGACCGTGTT CAAATCTGTC 
ACCAACAGGT TCATTAAAAA CCTGGCCTGC TCCGGGATTT GTGCCAGCGT GGTCTGCGTG 
CCCTTCGACA TCATCCTGAG CACCAGCCCT CACTGCTGCT GGTGGATCTA CACGATGCTC 
TTCTGCAAGG TGCTTAAATT CCTACACAAG GTGTTCTGCT CGGTGACTGT CCTCAGCTTT 
CCTGCCATTG CCCTGGACAG GTACTATTCA GTCCTCTACC CTCTGGAGAG AAAAATCTCT 
GACGCCAAGT CTCGGGAACT AGTGATGTAC ATCTGGGCCC ATGCAGTGGT GGCCAGCGTC 
CCTGTGTTTG CTGTGACCAA TGTAGCTGAC ATCTATGCTA CATCCACCTG CACGGAAGTC 
TGGAGCAACT CCTTGGGCCA CCTGGTGTAT GTCCTGATCT ACAACGTCAC CACGGTCATT 
GTGCCTGTGG CTGTGGTATT CCTCTTTCTT ATCCTGATCC GGAGGGCCCT GAGTGCCAGT 
CAGAAGAAGA AAGTCATCAT CGCTGCACTG AGGACCCCAC AGAACACCAT CTCTATCCCC 
TATGCATCCC AGCGGGAGGC AGAGCTACAT GCCACCCTGC TCTCCATGGT GACAGTTTTC 
ATCCTCTGCA GCGTACCCTA TGCCACCCTG GTTGTCTACC AGACTGTGCT CAATGTACCT 
AACACTTCTG TGTTCTTGCT TCTCACTGCT ATTTGGCTGC CCAAAGTCTC ACTGTTGGCC 
AACCCTGTGC TCTTCCTAAC TGTGAACAGA TCAGTACGCA AATGCTTGGT GGGGACCCTG 
GTGCAGTTGC ACCACCGGTA CAGTCGCCGC AATGTGGTTA GTACAGGGAG TGGGGTGGTT 
GAGCCTAGCC TTGAGCCCAG CATGCGCTCT GGTAGCCAGC TCTTGGAGAT GTTCCACATT 
GGACAGCAAC AGATCTTTAA GCCCTCGGAG GATGAGGAAG AGAGTGAGGC CAAGTATCTT 
GGCTCAACTG ATTTCCAGGC CAAGGAGGTA CTCACCTCCT CCCCAGAGGG CGAGGAGTCA 
CAGCTTGCAC CCTCTGTGCC GCCTCCAGGC ACAGTAGACT CTGTATCTCG GGTGTCCCCA 
GTAGCCCCCG TGGAACCTGG CATATTCCCT GATAAGTATT CCCTGCAGTT TGGCTTTGGG 
CCTTTCGAGT TGCCCCCTCA GTGGCTCTCT GAGACTCGAA ATAGCAAGAA GAGGCTGCTT 
CCCCCACTGG GCAATACCCC AGAAGAGCTG ATCCAGACAA AGGTGCCCAG AGTGAGCAGG 
GTGGAGCGGA AGATGAGCAG AAACAATAAA GTGAGCATTT TTCCAAAGGT AGACTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpr176 ( XM_017591437.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446926.1 Rattus norvegicus G protein-coupled receptor 176 (Gpr176), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa60242
Clone ID Related Accession (Same CDS sequence)	XM_017591435.1 , XM_017591436.1 , XM_017591437.1
Accession Version	XM_017591437.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	probable G-protein coupled receptor 176 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGGA ACTTCACGGT GTTATGGTCA ACCTGCCGCA CGACCGTGTT CAAATCTGTC 
ACCAACAGGT TCATTAAAAA CCTGGCCTGC TCCGGGATTT GTGCCAGCGT GGTCTGCGTG 
CCCTTCGACA TCATCCTGAG CACCAGCCCT CACTGCTGCT GGTGGATCTA CACGATGCTC 
TTCTGCAAGG TGCTTAAATT CCTACACAAG GTGTTCTGCT CGGTGACTGT CCTCAGCTTT 
CCTGCCATTG CCCTGGACAG GTACTATTCA GTCCTCTACC CTCTGGAGAG AAAAATCTCT 
GACGCCAAGT CTCGGGAACT AGTGATGTAC ATCTGGGCCC ATGCAGTGGT GGCCAGCGTC 
CCTGTGTTTG CTGTGACCAA TGTAGCTGAC ATCTATGCTA CATCCACCTG CACGGAAGTC 
TGGAGCAACT CCTTGGGCCA CCTGGTGTAT GTCCTGATCT ACAACGTCAC CACGGTCATT 
GTGCCTGTGG CTGTGGTATT CCTCTTTCTT ATCCTGATCC GGAGGGCCCT GAGTGCCAGT 
CAGAAGAAGA AAGTCATCAT CGCTGCACTG AGGACCCCAC AGAACACCAT CTCTATCCCC 
TATGCATCCC AGCGGGAGGC AGAGCTACAT GCCACCCTGC TCTCCATGGT GACAGTTTTC 
ATCCTCTGCA GCGTACCCTA TGCCACCCTG GTTGTCTACC AGACTGTGCT CAATGTACCT 
AACACTTCTG TGTTCTTGCT TCTCACTGCT ATTTGGCTGC CCAAAGTCTC ACTGTTGGCC 
AACCCTGTGC TCTTCCTAAC TGTGAACAGA TCAGTACGCA AATGCTTGGT GGGGACCCTG 
GTGCAGTTGC ACCACCGGTA CAGTCGCCGC AATGTGGTTA GTACAGGGAG TGGGGTGGTT 
GAGCCTAGCC TTGAGCCCAG CATGCGCTCT GGTAGCCAGC TCTTGGAGAT GTTCCACATT 
GGACAGCAAC AGATCTTTAA GCCCTCGGAG GATGAGGAAG AGAGTGAGGC CAAGTATCTT 
GGCTCAACTG ATTTCCAGGC CAAGGAGGTA CTCACCTCCT CCCCAGAGGG CGAGGAGTCA 
CAGCTTGCAC CCTCTGTGCC GCCTCCAGGC ACAGTAGACT CTGTATCTCG GGTGTCCCCA 
GTAGCCCCCG TGGAACCTGG CATATTCCCT GATAAGTATT CCCTGCAGTT TGGCTTTGGG 
CCTTTCGAGT TGCCCCCTCA GTGGCTCTCT GAGACTCGAA ATAGCAAGAA GAGGCTGCTT 
CCCCCACTGG GCAATACCCC AGAAGAGCTG ATCCAGACAA AGGTGCCCAG AGTGAGCAGG 
GTGGAGCGGA AGATGAGCAG AAACAATAAA GTGAGCATTT TTCCAAAGGT AGACTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446926.1
CDS	449..1828
Translation	MFGNFTVLWSTCRTTVFKSVTNRFIKNLACSGICASVVCVPFDIILSTSPHCCWWIYTMLFCKVLKFLHKVFCSVTVLSFPAIALDRYYSVLYPLERKISDAKSRELVMYIWAHAVVASVPVFAVTNVADIYATSTCTEVWSNSLGHLVYVLIYNVTTVIVPVAVVFLFLILIRRALSASQKKKVIIAALRTPQNTISIPYASQREAELHATLLSMVTVFILCSVPYATLVVYQTVLNVPNTSVFLLLTAIWLPKVSLLANPVLFLTVNRSVRKCLVGTLVQLHHRYSRRNVVSTGSGVVEPSLEPSMRSGSQLLEMFHIGQQQIFKPSEDEEESEAKYLGSTDFQAKEVLTSSPEGEESQLAPSVPPPGTVDSVSRVSPVAPVEPGIFPDKYSLQFGFGPFELPPQWLSETRNSKKRLLPPLGNTPEELIQTKVPRVSRVERKMSRNNKVSIFPKVDS

Target ORF information:

RefSeq Version	XM_017591437.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G protein-coupled receptor 176 (Gpr176), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591437.1

ORF Insert Sequence:

ATGTTTGGGA ACTTCACGGT GTTATGGTCA ACCTGCCGCA CGACCGTGTT CAAATCTGTC 
ACCAACAGGT TCATTAAAAA CCTGGCCTGC TCCGGGATTT GTGCCAGCGT GGTCTGCGTG 
CCCTTCGACA TCATCCTGAG CACCAGCCCT CACTGCTGCT GGTGGATCTA CACGATGCTC 
TTCTGCAAGG TGCTTAAATT CCTACACAAG GTGTTCTGCT CGGTGACTGT CCTCAGCTTT 
CCTGCCATTG CCCTGGACAG GTACTATTCA GTCCTCTACC CTCTGGAGAG AAAAATCTCT 
GACGCCAAGT CTCGGGAACT AGTGATGTAC ATCTGGGCCC ATGCAGTGGT GGCCAGCGTC 
CCTGTGTTTG CTGTGACCAA TGTAGCTGAC ATCTATGCTA CATCCACCTG CACGGAAGTC 
TGGAGCAACT CCTTGGGCCA CCTGGTGTAT GTCCTGATCT ACAACGTCAC CACGGTCATT 
GTGCCTGTGG CTGTGGTATT CCTCTTTCTT ATCCTGATCC GGAGGGCCCT GAGTGCCAGT 
CAGAAGAAGA AAGTCATCAT CGCTGCACTG AGGACCCCAC AGAACACCAT CTCTATCCCC 
TATGCATCCC AGCGGGAGGC AGAGCTACAT GCCACCCTGC TCTCCATGGT GACAGTTTTC 
ATCCTCTGCA GCGTACCCTA TGCCACCCTG GTTGTCTACC AGACTGTGCT CAATGTACCT 
AACACTTCTG TGTTCTTGCT TCTCACTGCT ATTTGGCTGC CCAAAGTCTC ACTGTTGGCC 
AACCCTGTGC TCTTCCTAAC TGTGAACAGA TCAGTACGCA AATGCTTGGT GGGGACCCTG 
GTGCAGTTGC ACCACCGGTA CAGTCGCCGC AATGTGGTTA GTACAGGGAG TGGGGTGGTT 
GAGCCTAGCC TTGAGCCCAG CATGCGCTCT GGTAGCCAGC TCTTGGAGAT GTTCCACATT 
GGACAGCAAC AGATCTTTAA GCCCTCGGAG GATGAGGAAG AGAGTGAGGC CAAGTATCTT 
GGCTCAACTG ATTTCCAGGC CAAGGAGGTA CTCACCTCCT CCCCAGAGGG CGAGGAGTCA 
CAGCTTGCAC CCTCTGTGCC GCCTCCAGGC ACAGTAGACT CTGTATCTCG GGTGTCCCCA 
GTAGCCCCCG TGGAACCTGG CATATTCCCT GATAAGTATT CCCTGCAGTT TGGCTTTGGG 
CCTTTCGAGT TGCCCCCTCA GTGGCTCTCT GAGACTCGAA ATAGCAAGAA GAGGCTGCTT 
CCCCCACTGG GCAATACCCC AGAAGAGCTG ATCCAGACAA AGGTGCCCAG AGTGAGCAGG 
GTGGAGCGGA AGATGAGCAG AAACAATAAA GTGAGCATTT TTCCAAAGGT AGACTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gpr176 is a Gz-linked orphan G-protein-coupled receptor that sets the pace of circadian behaviour.
Nature communications710583(2016 Feb)
Doi M,Murai I,Kunisue S,Setsu G,Uchio N,Tanaka R,Kobayashi S,Shimatani H,Hayashi H,Chao HW,Nakagawa Y,Takahashi Y,Hotta Y,Yasunaga J,Matsuoka M,Hastings MH,Kiyonari H,Okamura H

cDNA cloning of a putative G protein-coupled receptor from brain.
Biochimica et biophysica acta1261(1)121-5(1995 Mar)
Hata S,Emi Y,Iyanagi T,Osumi T

Molecular cloning of a novel putative G protein-coupled receptor from rat aortic smooth muscle. Downregulation of the mRNA level by the cyclic AMP messenger pathway.
Biochimica et biophysica acta1218(2)173-80(1994 Jun)
Ishizaka N,Okazaki H,Kurokawa K,Kumada M,Takuwa Y

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Gpr176 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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