Rab8a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rab8a
Entrez Gene ID	117103
Full Name	RAB8A, member RAS oncogene family
Synonyms	Mel,Rab8
General protein information	Preferred Names RAB8A, member RAS oncogene family Names ras-related protein Rab-8A mel transforming oncogene (derived from cell line NK14)- RAB8 homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16p14
Summary	member of low molecular weight (LMW) GTP-binding protein, rab family; may function in regulating neurotransmitter release [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053998.2	NP_446450.2	ras-related protein Rab-8A

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	rab8a	NP_001083031.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001211	XP_321946.4
Eremothecium gossypii	AGOS_AGL021W	NP_986645.1
Schizosaccharomyces pombe (fission yeast)	ypt2	NP_594580.1
Neurospora crassa	NCU06404	XP_962242.1
Arabidopsis thaliana (thale cress)	RAB8	NP_190929.1
Magnaporthe oryzae (rice blast fungus)	MGG_06135	XP_003712007.1
Oryza sativa (rice)	Os07g0239400	NP_001059259.1
Canis lupus familiaris (dog)	RAB8A	NP_001003152.1
Bos taurus (cattle)	RAB8A	NP_001098951.1
Gallus gallus (chicken)	RAB8A	NP_001026675.1
Drosophila melanogaster (fruit fly)	Rab8	NP_001246837.1
Saccharomyces cerevisiae (baker's yeast)	SEC4	NP_116650.1
Kluyveromyces lactis	KLLA0E12079g	XP_454494.1
Oryza sativa (rice)	Os05g0461300	NP_001055761.1
Xenopus tropicalis (tropical clawed frog)	rab8a	XP_002934841.1
Homo sapiens (human)	RAB8A	NP_005361.2
Pan troglodytes (chimpanzee)	RAB8A	NP_001229300.1
Rattus norvegicus (Norway rat)	Rab8a	NP_446450.2
Caenorhabditis elegans (roundworm)	rab-8	NP_491199.2
Arabidopsis thaliana (thale cress)	AT5G59840	NP_200792.1
Mus musculus (house mouse)	Rab8a	NP_075615.2
Arabidopsis thaliana (thale cress)	RAB8A	NP_001118780.1
Oryza sativa (rice)	Os03g0819900	NP_001051716.1

Gene Ontology
Bibliography

Related articles in PubMed

Rab8a/Rab11a regulate intercellular communications between neural cells via tunneling nanotubes.
Zhu H, Xue C, Xu X, Guo Y, Li X, Lu J, Ju S, Wang Y, Cao Z, Gu X
Cell death & disease7(12)e2523(2016 Dec)

Loss of C9ORF72 impairs autophagy and synergizes with polyQ Ataxin-2 to induce motor neuron dysfunction and cell death.
Sellier C, Campanari ML, Julie Corbier C, Gaucherot A, Kolb-Cheynel I, Oulad-Abdelghani M, Ruffenach F, Page A, Ciura S, Kabashi E, Charlet-Berguerand N
The EMBO journal35(12)1276-97(2016 06)

Ciliopathy-associated gene Cc2d2a promotes assembly of subdistal appendages on the mother centriole during cilia biogenesis.
Veleri S, Manjunath SH, Fariss RN, May-Simera H, Brooks M, Foskett TA, Gao C, Longo TA, Liu P, Nagashima K, Rachel RA, Li T, Dong L, Swaroop A
Nature communications54207(2014 Jun)

Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kr?hnert K, Sch?fer C, Rammner B, Koo SJ, Cla?en GA, Krauss M, Haucke V, Urlaub H, Rizzoli SO
Science (New York, N.Y.)344(6187)1023-8(2014 May)

Casein kinase 1? functions at the centrosome and Golgi to promote ciliogenesis.
Greer YE, Westlake CJ, Gao B, Bharti K, Shiba Y, Xavier CP, Pazour GJ, Yang Y, Rubin JS
Molecular biology of the cell25(10)1629-40(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rab8a or Rab11a might be involved in the formation of tunneling nanotubes structures in the peripheral nerve system.
Title: Rab8a/Rab11a regulate intercellular communications between neural cells via tunneling nanotubes.

Insulin signaling to the molecular switch Rab8A connects with the motor protein MyoVa to mobilize GLUT4 vesicles toward the muscle cell plasma membrane.
Title: Myosin Va mediates Rab8A-regulated GLUT4 vesicle exocytosis in insulin-stimulated muscle cells.

Results indicate that Rab11a, Rab8a, and Myo5B function as part of a network to promote stretch-induced exocytosis.
Title: A Rab11a-Rab8a-Myo5B network promotes stretch-regulated exocytosis in bladder umbrella cells.

Rab13 and Rab8A are Rab-GTPases activated by insulin and downstream of AS160 they regulate traffic of GLUT4 vesicles.
Title: Rab8A and Rab13 are activated by insulin and regulate GLUT4 translocation in muscle cells.

These results support a model whereby AS160, Rab8A, and myosin Vb are required for insulin-induced GLUT4 translocation in muscle cells, potentially as part of a linear signaling cascade.
Title: Muscle cells engage Rab8A and myosin Vb in insulin-dependent GLUT4 translocation.

The following Rab8a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rab8a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11286	NM_053998.2 Latest version!	Rattus norvegicus RAB8A, member RAS oncogene family (Rab8a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rab8a ( NM_053998.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446450.2 Rattus norvegicus RAB8A, member RAS oncogene family (Rab8a), mRNA.

Price & Availability↑

CloneID	ORa11286
Clone ID Related Accession (Same CDS sequence)	NM_053998.2
Accession Version	NM_053998.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	ras-related protein Rab-8A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC105863.1. On Dec 11, 2007 this sequence version replaced NM_053998.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC105863.1, BC071176.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906349, SAMN01906354 [ECO:0000348] ##Evidence-Data-END##

ATGGCGAAGA CCTACGATTA CCTGTTCAAG CTGCTGCTGA TCGGGGACTC GGGGGTAGGG 
AAGACCTGTG TCCTGTTCCG CTTCTCCGAG GACGCCTTCA ATTCCACGTT CATCTCTACC 
ATAGGAATTG ACTTTAAAAT TAGGACCATA GAGCTCGATG GCAAGAGGAT TAAACTGCAG 
ATATGGGACA CAGCCGGCCA GGAGCGGTTT CGGACAATCA CGACAGCCTA CTACAGGGGT 
GCCATGGGTA TCATGCTGGT CTACGACATT ACCAATGAGA AGTCCTTCGA CAACATCCGG 
AATTGGATTC GGAACATTGA GGAGCATGCC TCTGCAGATG TGGAGAAGAT GATACTGGGG 
AATAAGTGTG ATGTGAACGA CAAGAGGCAG GTGTCCAAGG AACGGGGAGA AAAGCTGGCA 
CTCGACTATG GGATCAAGTT CATGGAGACC AGCGCGAAGG CCAACATCAA TGTGGAGAAC 
GCATTTTTCA CTCTCGCCAG GGATATCAAA GCAAAAATGG ACAAAAAATT GGAAGGGAAC 
AGCCCACAGG GGAGCAGCCA TGGAGTCAAG ATCACAGTGG AGCAGCAGAA GAGGACCAGC 
TTCTTCCGGT GCAGTCTCCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446450.2
CDS	6..629
Translation	MAKTYDYLFKLLLIGDSGVGKTCVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIRNWIRNIEEHASADVEKMILGNKCDVNDKRQVSKERGEKLALDYGIKFMETSAKANINVENAFFTLARDIKAKMDKKLEGNSPQGSSHGVKITVEQQKRTSFFRCSLL

Target ORF information:

RefSeq Version	NM_053998.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RAB8A, member RAS oncogene family (Rab8a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053998.2

ORF Insert Sequence:

ATGGCGAAGA CCTACGATTA CCTGTTCAAG CTGCTGCTGA TCGGGGACTC GGGGGTAGGG 
AAGACCTGTG TCCTGTTCCG CTTCTCCGAG GACGCCTTCA ATTCCACGTT CATCTCTACC 
ATAGGAATTG ACTTTAAAAT TAGGACCATA GAGCTCGATG GCAAGAGGAT TAAACTGCAG 
ATATGGGACA CAGCCGGCCA GGAGCGGTTT CGGACAATCA CGACAGCCTA CTACAGGGGT 
GCCATGGGTA TCATGCTGGT CTACGACATT ACCAATGAGA AGTCCTTCGA CAACATCCGG 
AATTGGATTC GGAACATTGA GGAGCATGCC TCTGCAGATG TGGAGAAGAT GATACTGGGG 
AATAAGTGTG ATGTGAACGA CAAGAGGCAG GTGTCCAAGG AACGGGGAGA AAAGCTGGCA 
CTCGACTATG GGATCAAGTT CATGGAGACC AGCGCGAAGG CCAACATCAA TGTGGAGAAC 
GCATTTTTCA CTCTCGCCAG GGATATCAAA GCAAAAATGG ACAAAAAATT GGAAGGGAAC 
AGCCCACAGG GGAGCAGCCA TGGAGTCAAG ATCACAGTGG AGCAGCAGAA GAGGACCAGC 
TTCTTCCGGT GCAGTCTCCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Rab8a/Rab11a regulate intercellular communications between neural cells via tunneling nanotubes.
Cell death & disease7(12)e2523(2016 Dec)
Zhu H,Xue C,Xu X,Guo Y,Li X,Lu J,Ju S,Wang Y,Cao Z,Gu X

Loss of C9ORF72 impairs autophagy and synergizes with polyQ Ataxin-2 to induce motor neuron dysfunction and cell death.
The EMBO journal35(12)1276-97(2016 06)
Sellier C,Campanari ML,Julie Corbier C,Gaucherot A,Kolb-Cheynel I,Oulad-Abdelghani M,Ruffenach F,Page A,Ciura S,Kabashi E,Charlet-Berguerand N

Ciliopathy-associated gene Cc2d2a promotes assembly of subdistal appendages on the mother centriole during cilia biogenesis.
Nature communications54207(2014 Jun)
Veleri S,Manjunath SH,Fariss RN,May-Simera H,Brooks M,Foskett TA,Gao C,Longo TA,Liu P,Nagashima K,Rachel RA,Li T,Dong L,Swaroop A

Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Science (New York, N.Y.)344(6187)1023-8(2014 May)
Wilhelm BG,Mandad S,Truckenbrodt S,Kr?hnert K,Sch?fer C,Rammner B,Koo SJ,Cla?en GA,Krauss M,Haucke V,Urlaub H,Rizzoli SO

Casein kinase 1? functions at the centrosome and Golgi to promote ciliogenesis.
Molecular biology of the cell25(10)1629-40(2014 May)
Greer YE,Westlake CJ,Gao B,Bharti K,Shiba Y,Xavier CP,Pazour GJ,Yang Y,Rubin JS

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Rab8a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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