Nfasc gene cDNA ORF clone, Rattus norvegicus(Norway rat)

mRNA	Protein	Name
XM_017598647.1	XP_017454136.1	neurofascin isoform X12
XM_017598651.1	XP_017454140.1	neurofascin isoform X17
XM_017598641.1	XP_017454130.1	neurofascin isoform X5
XM_006249734.3	XP_006249796.1	neurofascin isoform X9
XM_017598639.1	XP_017454128.1	neurofascin isoform X3
XM_017598640.1	XP_017454129.1	neurofascin isoform X4
XM_006249738.3	XP_006249800.1	neurofascin isoform X14
XM_017598649.1	XP_017454138.1	neurofascin isoform X15
XM_017598652.1	XP_017454141.1	neurofascin isoform X17
XM_017598638.1	XP_017454127.1	neurofascin isoform X1
XM_017598645.1	XP_017454134.1	neurofascin isoform X10
XM_017598646.1	XP_017454135.1	neurofascin isoform X11
XM_017598648.1	XP_017454137.1	neurofascin isoform X13
XM_017598650.1	XP_017454139.1	neurofascin isoform X16
NM_001160314.1	NP_001153786.1	neurofascin isoform 1 precursor
XM_006249727.3	XP_006249789.1	neurofascin isoform X2
XM_017598644.1	XP_017454133.1	neurofascin isoform X8
NM_053909.2	NP_446361.1	neurofascin isoform 3 precursor
NM_001160315.1	NP_001153787.1	neurofascin isoform 4 precursor
XM_017598642.1	XP_017454131.1	neurofascin isoform X6
XM_017598643.1	XP_017454132.1	neurofascin isoform X7
NM_001160313.1	NP_001153785.1	neurofascin isoform 2 precursor

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa57977	XM_017598639.1 Latest version!	Rattus norvegicus neurofascin (Nfasc), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa57978	XM_017598640.1 Latest version!	Rattus norvegicus neurofascin (Nfasc), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa57979	XM_017598641.1 Latest version!	Rattus norvegicus neurofascin (Nfasc), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa57980	XM_017598642.1 Latest version!	Rattus norvegicus neurofascin (Nfasc), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa57981	XM_017598643.1 Latest version!	Rattus norvegicus neurofascin (Nfasc), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa57982	XM_017598644.1 Latest version!	Rattus norvegicus neurofascin (Nfasc), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa57983	XM_017598645.1 Latest version!	Rattus norvegicus neurofascin (Nfasc), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa57983	XM_017598646.1 Latest version!	Rattus norvegicus neurofascin (Nfasc), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa57984	XM_017598647.1 Latest version!	Rattus norvegicus neurofascin (Nfasc), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa57985	XM_017598648.1 Latest version!	Rattus norvegicus neurofascin (Nfasc), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa57986	XM_017598649.1 Latest version!	Rattus norvegicus neurofascin (Nfasc), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa57987	XM_017598650.1 Latest version!	Rattus norvegicus neurofascin (Nfasc), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa12769	NM_001160314.1 Latest version!	Rattus norvegicus neurofascin (Nfasc), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa12772	NM_001160315.1 Latest version!	Rattus norvegicus neurofascin (Nfasc), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa12778	NM_053909.2 Latest version!	Rattus norvegicus neurofascin (Nfasc), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa12781	NM_001160313.1 Latest version!	Rattus norvegicus neurofascin (Nfasc), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa35282	XM_006249738.3 Latest version!	Rattus norvegicus neurofascin (Nfasc), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa35271	XM_006249727.3 Latest version!	Rattus norvegicus neurofascin (Nfasc), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
ORa35271	XM_017598638.1 Latest version!	Rattus norvegicus neurofascin (Nfasc), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
ORa35278	XM_006249734.3 Latest version!	Rattus norvegicus neurofascin (Nfasc), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
ORa35285	XM_017598652.1 Latest version!	Rattus norvegicus neurofascin (Nfasc), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
ORa35285	XM_017598651.1 Latest version!	Rattus norvegicus neurofascin (Nfasc), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00

Nfasc ( XM_017598639.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454128.1 Rattus norvegicus neurofascin (Nfasc), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa57977
Clone ID Related Accession (Same CDS sequence)	XM_017598639.1
Accession Version	XM_017598639.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neurofascin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA 
TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA 
GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG 
AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG 
GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC 
CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCTGAC CCAACCCCCA 
ACGATCACCA AGCAGTCGGT GAAGGACCAC ATCGTGGACC CCCGAGATAA CATCCTGATT 
GAATGTGAAG CTAAAGGGAA CCCCGCCCCC AGTTTCCACT GGACTCGCAA CAGCAGGTTC 
TTCAACATTG CCAAGGACCC ACGGGTGTCC ATGAGGAGGA GGTCTGGGAC CTTGGTGATC 
GACTTCCGCA GTGGTGGGCG GCCTGAGGAG TACGAAGGGG AGTACCAGTG CTTTGCCCGG 
AACAAATTCG GCACAGCTCT TAGCAACCGC ATCCGCCTGC AGGTGTCCAA ATCTCCCCTG 
TGGCCCAAGG AAAACCTAGA CCCCGTCGTG GTTCAAGAGG GTGCCCCCTT AACCCTGCAG 
TGCAACCCCC CACCTGGCCT CCCATCCCCC GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AGGACAAGCG TGTCTCCCAG GGTCACAACG GGGACCTGTA CTTCTCCAAC 
GTCATGCTGC AGGACATGCA GACCGACTAC AGCTGCAATG CACGCTTCCA CTTCACCCAC 
ACCATTCAGC AGAAGAATCC CTTCACCCTC AAAGTCCTCA CCAACAACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATATAT AGTGCCCGAG GAGTTGCGGA AAGAACACCT 
AGCTTCATGT ATCCCCAGGG CACGTCAAGC AGTCAGATGG TACTGCGCGG CATGGACCTG 
CTGCTGGAGT GCATTGCCTC TGGGGTCCCA ACACCAGATA TTGCATGGTA CAAGAAAGGT 
GGGGACCTCC CATCTGACAA GGCCAAGTTC GAGAATTTTA ACAAGGCTCT GCGCATCACC 
AATGTCTCTG AAGAGGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGC 
ATCCGGCACA CGATCTCGGT GAGAGTAAAG GCTGCCCCAT ACTGGCTGGA TGAGCCCAAG 
AACTTGATCC TGGCTCCTGG TGAAGATGGG AGGCTGGTGT GTCGAGCCAA TGGGAACCCG 
AAGCCGACCG TCCAGTGGCT GGTGAATGGA GACCCTTTGC AATCGGCACC ACCCAACCCC 
AACCGTGAGG TGGCCGGAGA CACTATCATC TTCCGGGACA CTCAGATCAG CAGCAGGGCA 
GTGTACCAGT GTAACACATC CAACGAACAT GGCTACCTGC TGGCCAATGC CTTCGTCAGC 
GTATTAGATG TACCCCCTCG GATGCTGTCT CCCCGGAACC AGCTCATCAG GGTGATCCTT 
TACAACCGGA CGCGACTGGA CTGTCCGTTC TTTGGGTCTC CCATTCCAAC ACTCCGATGG 
TTTAAGAATG GGCAAGGAAG CAACCTGGAT GGTGGTAACT ACCACGTCTA CGAAAATGGC 
AGCCTGGAAA TCAAGATGAT TCGCAAAGAG GACCAAGGCA TCTACACCTG TGTGGCCACC 
AACATCCTGG GAAAAGCTGA AAATCAAGTC CGCCTGGAGG TCAAAGACCC CACCAGGATC 
TACAGGATGC CTGAAGACCA GGTGGCCAAG AGGGGCACCA CAGTGCAGCT GGAGTGCCGT 
GTGAAGCATG ACCCCTCCTT GAAACTCACA GTCTCCTGGC TGAAGGATGA CGAGCCACTC 
TACATTGGAA ACAGGATGAA AAAGGAAGAT GACTCCCTGA CCATCTTCGG AGTGGCAGAG 
CGGGACCAAG GCAGTTACAC GTGCATGGCC AGCACCGAGC TGGACCAGGA CCTGGCAAAG 
GCCTACCTCA CTGTTCTAGC TGATCAGGCC ACTCCGACTA ACCGTTTGGC TGCCCTGCCC 
AAAGGGCGGC CAGACCGACC CAGGGACCTG GAGCTCACTG ACCTGGCGGA AAGGAGTGTG 
AGGCTGACCT GGATCCCGGG GGATGATAAC AACAGTCCCA TCACAGACTA CGTTGTCCAG 
TTTGAAGAGG ACCAGTTCCA GCCAGGAGTC TGGCATGACC ACTCCAAGTT CCCAGGCAGT 
GTCAACTCAG CCGTCCTCCA TCTGTCCCCG TATGTCAACT ATCAGTTCCG AGTCATCGCT 
GTCAACGAGG TTGGGAGCAG CCACCCCAGC CTTCCATCCG AGCGGTACCG AACAAGCGGG 
GCACCCCCTG AATCCAACCC CAGTGATGTG AAGGGCGAAG GGACACGAAA AAACAATATG 
GAGATCACAT GGACGCCTAT GAATGCTACC TCCGCCTTTG GCCCCAACCT GCGCTACATT 
GTCAAGTGGC GACGGAGAGA AACCCGAGAG ACTTGGAACA ATGTCACCGT GTGGGGCTCT 
CGCTATGTGG TGGGGCAGAC CCCTGTCTAC GTACCCTATG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAAGGCCC GGAGCCTGAA ACCGTCATTG GGTACTCGGG GGAAGATTAT 
CCCAGGGCTG CACCCACTGA AGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC TGACACGGTC CAGGGCCAGC TCAGAGAGTA TCGAGCTTAC 
TACTGGAGGG AAAGCAGTTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAGGCC 
AGCTTCCCTG GCGACCGCCC CCGGGGCGTG GTGGGTCGCC TGTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGAGA GGTGATGGGC CTCGAAGTGA AACCAAGGAA 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCAGAGTCCG ACAGCCCAAC 
CTGGAGACCA TCAACCTGGA ATGGGATCAC CCAGAGCACC CCAACGGGAT CCTGATTGGA 
TACACGCTCA GATACGTGCC CTTTAATGGA ACCAAACTGG GAAAGCAGAT GGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCTCGGTG CAGAGAGCAG ACCCCGTGTC CCGTTACCGC 
TTCTCCCTCA GTGCCAGGAC GCAGGTGGGC TCTGGAGAAG CAGCCACGGA GGAGTCCCCA 
ACACCTCCAA ATGAAGCTAC TCCAACTGCA GCTCCTCCCA CATTGCCCCC GACTACCGTG 
GGTACCACAG GCCTTGTGAG CAGTACTGAT GCTACTGCCC TTGCTGCCAC CAGTGAAGCC 
ACAACAGTTC CCATCATCCC AACCGTCGTA CCTACCACCG TCGCCACCAC CATTGCCACA 
ACTACTACAA CCACTGCCGC CGCCACCACC ACTACCACCA CGGAGAGTCC TCCCACTACC 
ACCACTGGGA CTAAGATTCA CGAAACCGCC CCCGACGAGC AGTCCATTTG GAACGTCACG 
GTGCTCCCCA ACAGTAAATG GGCCAACATC ACCTGGAAGC ACAATTTCAG GCCTGGAACT 
GACTTTGTGG TTGAGTACAT CGACAGCAAC CATACGAAAA AAACTGTCCC TGTTAAGGCC 
CAAGCCCAGC CCATACAGCT GACAGACCTC TTTCCCGGGA TGACGTACAC GTTGCGGGTG 
TATTCCCGGG ACAACGAGGG CATCAGCAGT ACCGTCATCA CCTTTATGAC CAGTACAGCT 
TACACCAACA ACCAGACTGA CATCGCCACC CAGGGCTGGT TCATCGGGCT CATGTGTGCC 
ATTGCCCTTC TGGTGCTGAT CCTGCTTATC GTCTGCTTCA TCAAGAGGAG TCGTGGCGGC 
AAGTACCCAG TGCGAGAAAA GAAGGATGTC CCCTTGGGCC CTGAAGACCC CAAAGAAGAA 
GATGGTTCAT TTGACTACAG TGACGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACATCT 
CTGGACGGCA CCATCAAGCA GCAGGAGAGT GACGACAGCC TAGTGGACTA CGGCGAGGGT 
GGCGAGGGCC AGTTCAACGA AGATGGCTCC TTTATTGGCC AGTACACTGT CAGAAAGGAC 
AAGGAGGAGA CCGAGGGCAA TGAGAGCTCA GAGGCCACAT CTCCAGTCAA TGCCATCTAT 
TCCCTGGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454128.1
CDS	53..4444
Translation	MRRLAARAAAGDAGEVAAPGADIWDCAQGWSWSSWRLTDLCAIWEPGVFLPSAAAVNRSRASAGVAKSRPNEAEKLSTVWKAHEERRKASRARGLGSAEQGLALRMARQQAPPWVHVALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWLVNGDPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPETVIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVGRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPTPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAAATTTTTTESPPTTTTGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQTDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVRKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017598639.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurofascin (Nfasc), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598639.1

ORF Insert Sequence:

ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA 
TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA 
GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG 
AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG 
GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC 
CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCTGAC CCAACCCCCA 
ACGATCACCA AGCAGTCGGT GAAGGACCAC ATCGTGGACC CCCGAGATAA CATCCTGATT 
GAATGTGAAG CTAAAGGGAA CCCCGCCCCC AGTTTCCACT GGACTCGCAA CAGCAGGTTC 
TTCAACATTG CCAAGGACCC ACGGGTGTCC ATGAGGAGGA GGTCTGGGAC CTTGGTGATC 
GACTTCCGCA GTGGTGGGCG GCCTGAGGAG TACGAAGGGG AGTACCAGTG CTTTGCCCGG 
AACAAATTCG GCACAGCTCT TAGCAACCGC ATCCGCCTGC AGGTGTCCAA ATCTCCCCTG 
TGGCCCAAGG AAAACCTAGA CCCCGTCGTG GTTCAAGAGG GTGCCCCCTT AACCCTGCAG 
TGCAACCCCC CACCTGGCCT CCCATCCCCC GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AGGACAAGCG TGTCTCCCAG GGTCACAACG GGGACCTGTA CTTCTCCAAC 
GTCATGCTGC AGGACATGCA GACCGACTAC AGCTGCAATG CACGCTTCCA CTTCACCCAC 
ACCATTCAGC AGAAGAATCC CTTCACCCTC AAAGTCCTCA CCAACAACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATATAT AGTGCCCGAG GAGTTGCGGA AAGAACACCT 
AGCTTCATGT ATCCCCAGGG CACGTCAAGC AGTCAGATGG TACTGCGCGG CATGGACCTG 
CTGCTGGAGT GCATTGCCTC TGGGGTCCCA ACACCAGATA TTGCATGGTA CAAGAAAGGT 
GGGGACCTCC CATCTGACAA GGCCAAGTTC GAGAATTTTA ACAAGGCTCT GCGCATCACC 
AATGTCTCTG AAGAGGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGC 
ATCCGGCACA CGATCTCGGT GAGAGTAAAG GCTGCCCCAT ACTGGCTGGA TGAGCCCAAG 
AACTTGATCC TGGCTCCTGG TGAAGATGGG AGGCTGGTGT GTCGAGCCAA TGGGAACCCG 
AAGCCGACCG TCCAGTGGCT GGTGAATGGA GACCCTTTGC AATCGGCACC ACCCAACCCC 
AACCGTGAGG TGGCCGGAGA CACTATCATC TTCCGGGACA CTCAGATCAG CAGCAGGGCA 
GTGTACCAGT GTAACACATC CAACGAACAT GGCTACCTGC TGGCCAATGC CTTCGTCAGC 
GTATTAGATG TACCCCCTCG GATGCTGTCT CCCCGGAACC AGCTCATCAG GGTGATCCTT 
TACAACCGGA CGCGACTGGA CTGTCCGTTC TTTGGGTCTC CCATTCCAAC ACTCCGATGG 
TTTAAGAATG GGCAAGGAAG CAACCTGGAT GGTGGTAACT ACCACGTCTA CGAAAATGGC 
AGCCTGGAAA TCAAGATGAT TCGCAAAGAG GACCAAGGCA TCTACACCTG TGTGGCCACC 
AACATCCTGG GAAAAGCTGA AAATCAAGTC CGCCTGGAGG TCAAAGACCC CACCAGGATC 
TACAGGATGC CTGAAGACCA GGTGGCCAAG AGGGGCACCA CAGTGCAGCT GGAGTGCCGT 
GTGAAGCATG ACCCCTCCTT GAAACTCACA GTCTCCTGGC TGAAGGATGA CGAGCCACTC 
TACATTGGAA ACAGGATGAA AAAGGAAGAT GACTCCCTGA CCATCTTCGG AGTGGCAGAG 
CGGGACCAAG GCAGTTACAC GTGCATGGCC AGCACCGAGC TGGACCAGGA CCTGGCAAAG 
GCCTACCTCA CTGTTCTAGC TGATCAGGCC ACTCCGACTA ACCGTTTGGC TGCCCTGCCC 
AAAGGGCGGC CAGACCGACC CAGGGACCTG GAGCTCACTG ACCTGGCGGA AAGGAGTGTG 
AGGCTGACCT GGATCCCGGG GGATGATAAC AACAGTCCCA TCACAGACTA CGTTGTCCAG 
TTTGAAGAGG ACCAGTTCCA GCCAGGAGTC TGGCATGACC ACTCCAAGTT CCCAGGCAGT 
GTCAACTCAG CCGTCCTCCA TCTGTCCCCG TATGTCAACT ATCAGTTCCG AGTCATCGCT 
GTCAACGAGG TTGGGAGCAG CCACCCCAGC CTTCCATCCG AGCGGTACCG AACAAGCGGG 
GCACCCCCTG AATCCAACCC CAGTGATGTG AAGGGCGAAG GGACACGAAA AAACAATATG 
GAGATCACAT GGACGCCTAT GAATGCTACC TCCGCCTTTG GCCCCAACCT GCGCTACATT 
GTCAAGTGGC GACGGAGAGA AACCCGAGAG ACTTGGAACA ATGTCACCGT GTGGGGCTCT 
CGCTATGTGG TGGGGCAGAC CCCTGTCTAC GTACCCTATG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAAGGCCC GGAGCCTGAA ACCGTCATTG GGTACTCGGG GGAAGATTAT 
CCCAGGGCTG CACCCACTGA AGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC TGACACGGTC CAGGGCCAGC TCAGAGAGTA TCGAGCTTAC 
TACTGGAGGG AAAGCAGTTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAGGCC 
AGCTTCCCTG GCGACCGCCC CCGGGGCGTG GTGGGTCGCC TGTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGAGA GGTGATGGGC CTCGAAGTGA AACCAAGGAA 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCAGAGTCCG ACAGCCCAAC 
CTGGAGACCA TCAACCTGGA ATGGGATCAC CCAGAGCACC CCAACGGGAT CCTGATTGGA 
TACACGCTCA GATACGTGCC CTTTAATGGA ACCAAACTGG GAAAGCAGAT GGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCTCGGTG CAGAGAGCAG ACCCCGTGTC CCGTTACCGC 
TTCTCCCTCA GTGCCAGGAC GCAGGTGGGC TCTGGAGAAG CAGCCACGGA GGAGTCCCCA 
ACACCTCCAA ATGAAGCTAC TCCAACTGCA GCTCCTCCCA CATTGCCCCC GACTACCGTG 
GGTACCACAG GCCTTGTGAG CAGTACTGAT GCTACTGCCC TTGCTGCCAC CAGTGAAGCC 
ACAACAGTTC CCATCATCCC AACCGTCGTA CCTACCACCG TCGCCACCAC CATTGCCACA 
ACTACTACAA CCACTGCCGC CGCCACCACC ACTACCACCA CGGAGAGTCC TCCCACTACC 
ACCACTGGGA CTAAGATTCA CGAAACCGCC CCCGACGAGC AGTCCATTTG GAACGTCACG 
GTGCTCCCCA ACAGTAAATG GGCCAACATC ACCTGGAAGC ACAATTTCAG GCCTGGAACT 
GACTTTGTGG TTGAGTACAT CGACAGCAAC CATACGAAAA AAACTGTCCC TGTTAAGGCC 
CAAGCCCAGC CCATACAGCT GACAGACCTC TTTCCCGGGA TGACGTACAC GTTGCGGGTG 
TATTCCCGGG ACAACGAGGG CATCAGCAGT ACCGTCATCA CCTTTATGAC CAGTACAGCT 
TACACCAACA ACCAGACTGA CATCGCCACC CAGGGCTGGT TCATCGGGCT CATGTGTGCC 
ATTGCCCTTC TGGTGCTGAT CCTGCTTATC GTCTGCTTCA TCAAGAGGAG TCGTGGCGGC 
AAGTACCCAG TGCGAGAAAA GAAGGATGTC CCCTTGGGCC CTGAAGACCC CAAAGAAGAA 
GATGGTTCAT TTGACTACAG TGACGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACATCT 
CTGGACGGCA CCATCAAGCA GCAGGAGAGT GACGACAGCC TAGTGGACTA CGGCGAGGGT 
GGCGAGGGCC AGTTCAACGA AGATGGCTCC TTTATTGGCC AGTACACTGT CAGAAAGGAC 
AAGGAGGAGA CCGAGGGCAA TGAGAGCTCA GAGGCCACAT CTCCAGTCAA TGCCATCTAT 
TCCCTGGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_017598640.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454129.1 Rattus norvegicus neurofascin (Nfasc), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa57978
Clone ID Related Accession (Same CDS sequence)	XM_017598640.1
Accession Version	XM_017598640.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neurofascin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA 
TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA 
GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG 
AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG 
GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC 
CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT 
GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC 
CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG 
ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG 
TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG 
TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG 
GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT 
GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG 
GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA 
CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC 
AACAACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATATATAG TGCCCGAGGA 
GTTGCGGAAA GAACACCTAG CTTCATGTAT CCCCAGGGCA CGTCAAGCAG TCAGATGGTA 
CTGCGCGGCA TGGACCTGCT GCTGGAGTGC ATTGCCTCTG GGGTCCCAAC ACCAGATATT 
GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTCGA GAATTTTAAC 
AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCCCCATAC 
TGGCTGGATG AGCCCAAGAA CTTGATCCTG GCTCCTGGTG AAGATGGGAG GCTGGTGTGT 
CGAGCCAATG GGAACCCGAA GCCGACCGTC CAGTGGCTGG TGAATGGAGA CCCTTTGCAA 
TCGGCACCAC CCAACCCCAA CCGTGAGGTG GCCGGAGACA CTATCATCTT CCGGGACACT 
CAGATCAGCA GCAGGGCAGT GTACCAGTGT AACACATCCA ACGAACATGG CTACCTGCTG 
GCCAATGCCT TCGTCAGCGT ATTAGATGTA CCCCCTCGGA TGCTGTCTCC CCGGAACCAG 
CTCATCAGGG TGATCCTTTA CAACCGGACG CGACTGGACT GTCCGTTCTT TGGGTCTCCC 
ATTCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG TGGTAACTAC 
CACGTCTACG AAAATGGCAG CCTGGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC 
TACACCTGTG TGGCCACCAA CATCCTGGGA AAAGCTGAAA ATCAAGTCCG CCTGGAGGTC 
AAAGACCCCA CCAGGATCTA CAGGATGCCT GAAGACCAGG TGGCCAAGAG GGGCACCACA 
GTGCAGCTGG AGTGCCGTGT GAAGCATGAC CCCTCCTTGA AACTCACAGT CTCCTGGCTG 
AAGGATGACG AGCCACTCTA CATTGGAAAC AGGATGAAAA AGGAAGATGA CTCCCTGACC 
ATCTTCGGAG TGGCAGAGCG GGACCAAGGC AGTTACACGT GCATGGCCAG CACCGAGCTG 
GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGGGC GGCCAGACCG ACCCAGGGAC 
CTGGAGCTCA CTGACCTGGC GGAAAGGAGT GTGAGGCTGA CCTGGATCCC GGGGGATGAT 
AACAACAGTC CCATCACAGA CTACGTTGTC CAGTTTGAAG AGGACCAGTT CCAGCCAGGA 
GTCTGGCATG ACCACTCCAA GTTCCCAGGC AGTGTCAACT CAGCCGTCCT CCATCTGTCC 
CCGTATGTCA ACTATCAGTT CCGAGTCATC GCTGTCAACG AGGTTGGGAG CAGCCACCCC 
AGCCTTCCAT CCGAGCGGTA CCGAACAAGC GGGGCACCCC CTGAATCCAA CCCCAGTGAT 
GTGAAGGGCG AAGGGACACG AAAAAACAAT ATGGAGATCA CATGGACGCC TATGAATGCT 
ACCTCCGCCT TTGGCCCCAA CCTGCGCTAC ATTGTCAAGT GGCGACGGAG AGAAACCCGA 
GAGACTTGGA ACAATGTCAC CGTGTGGGGC TCTCGCTATG TGGTGGGGCA GACCCCTGTC 
TACGTACCCT ATGAGATCCG AGTCCAGGCT GAAAATGACT TTGGGAAAGG CCCGGAGCCT 
GAAACCGTCA TTGGGTACTC GGGGGAAGAT TATCCCAGGG CTGCACCCAC TGAAGTTAAA 
ATCCGAGTCC TGAACAGCAC AGCCATCAGC CTTCAGTGGA ACCGCGTCTA CTCTGACACG 
GTCCAGGGCC AGCTCAGAGA GTATCGAGCT TACTACTGGA GGGAAAGCAG TTTGCTGAAG 
AACCTGTGGG TGTCTCAGAA GAGACAGCAG GCCAGCTTCC CTGGCGACCG CCCCCGGGGC 
GTGGTGGGTC GCCTGTTCCC CTACAGTAAC TACAAGCTGG AGATGGTTGT GGTCAATGGG 
AGAGGTGATG GGCCTCGAAG TGAAACCAAG GAATTCACCA CCCCGGAAGG AGTACCCAGT 
GCCCCCAGGC GTTTCAGAGT CCGACAGCCC AACCTGGAGA CCATCAACCT GGAATGGGAT 
CACCCAGAGC ACCCCAACGG GATCCTGATT GGATACACGC TCAGATACGT GCCCTTTAAT 
GGAACCAAAC TGGGAAAGCA GATGGTGGAA AACTTCTCTC CCAATCAGAC CAAGTTCTCG 
GTGCAGAGAG CAGACCCCGT GTCCCGTTAC CGCTTCTCCC TCAGTGCCAG GACGCAGGTG 
GGCTCTGGAG AAGCAGCCAC GGAGGAGTCC CCAACACCTC CAAATGAAGC TACTCCAACT 
GCAGCTCCTC CCACATTGCC CCCGACTACC GTGGGTACCA CAGGCCTTGT GAGCAGTACT 
GATGCTACTG CCCTTGCTGC CACCAGTGAA GCCACAACAG TTCCCATCAT CCCAACCGTC 
GTACCTACCA CCGTCGCCAC CACCATTGCC ACAACTACTA CAACCACTGC CGCCGCCACC 
ACCACTACCA CCACGGAGAG TCCTCCCACT ACCACCACTG GGACTAAGAT TCACGAAACC 
GCCCCCGACG AGCAGTCCAT TTGGAACGTC ACGGTGCTCC CCAACAGTAA ATGGGCCAAC 
ATCACCTGGA AGCACAATTT CAGGCCTGGA ACTGACTTTG TGGTTGAGTA CATCGACAGC 
AACCATACGA AAAAAACTGT CCCTGTTAAG GCCCAAGCCC AGCCCATACA GCTGACAGAC 
CTCTTTCCCG GGATGACGTA CACGTTGCGG GTGTATTCCC GGGACAACGA GGGCATCAGC 
AGTACCGTCA TCACCTTTAT GACCAGTACA GCTTACACCA ACAACCAGAC TGACATCGCC 
ACCCAGGGCT GGTTCATCGG GCTCATGTGT GCCATTGCCC TTCTGGTGCT GATCCTGCTT 
ATCGTCTGCT TCATCAAGAG GAGTCGTGGC GGCAAGTACC CAGTGCGAGA AAAGAAGGAT 
GTCCCCTTGG GCCCTGAAGA CCCCAAAGAA GAAGATGGTT CATTTGACTA CAGTGACGAG 
GACAACAAGC CCCTGCAGGG CAGCCAGACA TCTCTGGACG GCACCATCAA GCAGCAGGAG 
AGTGACGACA GCCTAGTGGA CTACGGCGAG GGTGGCGAGG GCCAGTTCAA CGAAGATGGC 
TCCTTTATTG GCCAGTACAC TGTCAGAAAG GACAAGGAGG AGACCGAGGG CAATGAGAGC 
TCAGAGGCCA CATCTCCAGT CAATGCCATC TATTCCCTGG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454129.1
CDS	53..4417
Translation	MRRLAARAAAGDAGEVAAPGADIWDCAQGWSWSSWRLTDLCAIWEPGVFLPSAAAVNRSRASAGVAKSRPNEAEKLSTVWKAHEERRKASRARGLGSAEQGLALRMARQQAPPWVHVALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWLVNGDPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPETVIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVGRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPTPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAAATTTTTTESPPTTTTGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQTDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVRKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017598640.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurofascin (Nfasc), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598640.1

ORF Insert Sequence:

ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA 
TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA 
GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG 
AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG 
GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC 
CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT 
GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC 
CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG 
ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG 
TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG 
TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG 
GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT 
GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG 
GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA 
CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC 
AACAACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATATATAG TGCCCGAGGA 
GTTGCGGAAA GAACACCTAG CTTCATGTAT CCCCAGGGCA CGTCAAGCAG TCAGATGGTA 
CTGCGCGGCA TGGACCTGCT GCTGGAGTGC ATTGCCTCTG GGGTCCCAAC ACCAGATATT 
GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTCGA GAATTTTAAC 
AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCCCCATAC 
TGGCTGGATG AGCCCAAGAA CTTGATCCTG GCTCCTGGTG AAGATGGGAG GCTGGTGTGT 
CGAGCCAATG GGAACCCGAA GCCGACCGTC CAGTGGCTGG TGAATGGAGA CCCTTTGCAA 
TCGGCACCAC CCAACCCCAA CCGTGAGGTG GCCGGAGACA CTATCATCTT CCGGGACACT 
CAGATCAGCA GCAGGGCAGT GTACCAGTGT AACACATCCA ACGAACATGG CTACCTGCTG 
GCCAATGCCT TCGTCAGCGT ATTAGATGTA CCCCCTCGGA TGCTGTCTCC CCGGAACCAG 
CTCATCAGGG TGATCCTTTA CAACCGGACG CGACTGGACT GTCCGTTCTT TGGGTCTCCC 
ATTCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG TGGTAACTAC 
CACGTCTACG AAAATGGCAG CCTGGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC 
TACACCTGTG TGGCCACCAA CATCCTGGGA AAAGCTGAAA ATCAAGTCCG CCTGGAGGTC 
AAAGACCCCA CCAGGATCTA CAGGATGCCT GAAGACCAGG TGGCCAAGAG GGGCACCACA 
GTGCAGCTGG AGTGCCGTGT GAAGCATGAC CCCTCCTTGA AACTCACAGT CTCCTGGCTG 
AAGGATGACG AGCCACTCTA CATTGGAAAC AGGATGAAAA AGGAAGATGA CTCCCTGACC 
ATCTTCGGAG TGGCAGAGCG GGACCAAGGC AGTTACACGT GCATGGCCAG CACCGAGCTG 
GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGGGC GGCCAGACCG ACCCAGGGAC 
CTGGAGCTCA CTGACCTGGC GGAAAGGAGT GTGAGGCTGA CCTGGATCCC GGGGGATGAT 
AACAACAGTC CCATCACAGA CTACGTTGTC CAGTTTGAAG AGGACCAGTT CCAGCCAGGA 
GTCTGGCATG ACCACTCCAA GTTCCCAGGC AGTGTCAACT CAGCCGTCCT CCATCTGTCC 
CCGTATGTCA ACTATCAGTT CCGAGTCATC GCTGTCAACG AGGTTGGGAG CAGCCACCCC 
AGCCTTCCAT CCGAGCGGTA CCGAACAAGC GGGGCACCCC CTGAATCCAA CCCCAGTGAT 
GTGAAGGGCG AAGGGACACG AAAAAACAAT ATGGAGATCA CATGGACGCC TATGAATGCT 
ACCTCCGCCT TTGGCCCCAA CCTGCGCTAC ATTGTCAAGT GGCGACGGAG AGAAACCCGA 
GAGACTTGGA ACAATGTCAC CGTGTGGGGC TCTCGCTATG TGGTGGGGCA GACCCCTGTC 
TACGTACCCT ATGAGATCCG AGTCCAGGCT GAAAATGACT TTGGGAAAGG CCCGGAGCCT 
GAAACCGTCA TTGGGTACTC GGGGGAAGAT TATCCCAGGG CTGCACCCAC TGAAGTTAAA 
ATCCGAGTCC TGAACAGCAC AGCCATCAGC CTTCAGTGGA ACCGCGTCTA CTCTGACACG 
GTCCAGGGCC AGCTCAGAGA GTATCGAGCT TACTACTGGA GGGAAAGCAG TTTGCTGAAG 
AACCTGTGGG TGTCTCAGAA GAGACAGCAG GCCAGCTTCC CTGGCGACCG CCCCCGGGGC 
GTGGTGGGTC GCCTGTTCCC CTACAGTAAC TACAAGCTGG AGATGGTTGT GGTCAATGGG 
AGAGGTGATG GGCCTCGAAG TGAAACCAAG GAATTCACCA CCCCGGAAGG AGTACCCAGT 
GCCCCCAGGC GTTTCAGAGT CCGACAGCCC AACCTGGAGA CCATCAACCT GGAATGGGAT 
CACCCAGAGC ACCCCAACGG GATCCTGATT GGATACACGC TCAGATACGT GCCCTTTAAT 
GGAACCAAAC TGGGAAAGCA GATGGTGGAA AACTTCTCTC CCAATCAGAC CAAGTTCTCG 
GTGCAGAGAG CAGACCCCGT GTCCCGTTAC CGCTTCTCCC TCAGTGCCAG GACGCAGGTG 
GGCTCTGGAG AAGCAGCCAC GGAGGAGTCC CCAACACCTC CAAATGAAGC TACTCCAACT 
GCAGCTCCTC CCACATTGCC CCCGACTACC GTGGGTACCA CAGGCCTTGT GAGCAGTACT 
GATGCTACTG CCCTTGCTGC CACCAGTGAA GCCACAACAG TTCCCATCAT CCCAACCGTC 
GTACCTACCA CCGTCGCCAC CACCATTGCC ACAACTACTA CAACCACTGC CGCCGCCACC 
ACCACTACCA CCACGGAGAG TCCTCCCACT ACCACCACTG GGACTAAGAT TCACGAAACC 
GCCCCCGACG AGCAGTCCAT TTGGAACGTC ACGGTGCTCC CCAACAGTAA ATGGGCCAAC 
ATCACCTGGA AGCACAATTT CAGGCCTGGA ACTGACTTTG TGGTTGAGTA CATCGACAGC 
AACCATACGA AAAAAACTGT CCCTGTTAAG GCCCAAGCCC AGCCCATACA GCTGACAGAC 
CTCTTTCCCG GGATGACGTA CACGTTGCGG GTGTATTCCC GGGACAACGA GGGCATCAGC 
AGTACCGTCA TCACCTTTAT GACCAGTACA GCTTACACCA ACAACCAGAC TGACATCGCC 
ACCCAGGGCT GGTTCATCGG GCTCATGTGT GCCATTGCCC TTCTGGTGCT GATCCTGCTT 
ATCGTCTGCT TCATCAAGAG GAGTCGTGGC GGCAAGTACC CAGTGCGAGA AAAGAAGGAT 
GTCCCCTTGG GCCCTGAAGA CCCCAAAGAA GAAGATGGTT CATTTGACTA CAGTGACGAG 
GACAACAAGC CCCTGCAGGG CAGCCAGACA TCTCTGGACG GCACCATCAA GCAGCAGGAG 
AGTGACGACA GCCTAGTGGA CTACGGCGAG GGTGGCGAGG GCCAGTTCAA CGAAGATGGC 
TCCTTTATTG GCCAGTACAC TGTCAGAAAG GACAAGGAGG AGACCGAGGG CAATGAGAGC 
TCAGAGGCCA CATCTCCAGT CAATGCCATC TATTCCCTGG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_017598641.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454130.1 Rattus norvegicus neurofascin (Nfasc), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa57979
Clone ID Related Accession (Same CDS sequence)	XM_017598641.1
Accession Version	XM_017598641.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neurofascin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA 
TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA 
GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG 
AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG 
GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC 
CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT 
GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC 
CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG 
ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG 
TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG 
TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG 
GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT 
GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG 
GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA 
CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC 
ACCCGAGGAG TTGCGGAAAG AACACCTAGC TTCATGTATC CCCAGGGCAC GTCAAGCAGT 
CAGATGGTAC TGCGCGGCAT GGACCTGCTG CTGGAGTGCA TTGCCTCTGG GGTCCCAACA 
CCAGATATTG CATGGTACAA GAAAGGTGGG GACCTCCCAT CTGACAAGGC CAAGTTCGAG 
AATTTTAACA AGGCTCTGCG CATCACCAAT GTCTCTGAAG AGGACTCTGG GGAGTATTTC 
TGCCTGGCCT CCAACAAGAT GGGCAGCATC CGGCACACGA TCTCGGTGAG AGTAAAGGCT 
GCCCCATACT GGCTGGATGA GCCCAAGAAC TTGATCCTGG CTCCTGGTGA AGATGGGAGG 
CTGGTGTGTC GAGCCAATGG GAACCCGAAG CCGACCGTCC AGTGGCTGGT GAATGGAGAC 
CCTTTGCAAT CGGCACCACC CAACCCCAAC CGTGAGGTGG CCGGAGACAC TATCATCTTC 
CGGGACACTC AGATCAGCAG CAGGGCAGTG TACCAGTGTA ACACATCCAA CGAACATGGC 
TACCTGCTGG CCAATGCCTT CGTCAGCGTA TTAGATGTAC CCCCTCGGAT GCTGTCTCCC 
CGGAACCAGC TCATCAGGGT GATCCTTTAC AACCGGACGC GACTGGACTG TCCGTTCTTT 
GGGTCTCCCA TTCCAACACT CCGATGGTTT AAGAATGGGC AAGGAAGCAA CCTGGATGGT 
GGTAACTACC ACGTCTACGA AAATGGCAGC CTGGAAATCA AGATGATTCG CAAAGAGGAC 
CAAGGCATCT ACACCTGTGT GGCCACCAAC ATCCTGGGAA AAGCTGAAAA TCAAGTCCGC 
CTGGAGGTCA AAGACCCCAC CAGGATCTAC AGGATGCCTG AAGACCAGGT GGCCAAGAGG 
GGCACCACAG TGCAGCTGGA GTGCCGTGTG AAGCATGACC CCTCCTTGAA ACTCACAGTC 
TCCTGGCTGA AGGATGACGA GCCACTCTAC ATTGGAAACA GGATGAAAAA GGAAGATGAC 
TCCCTGACCA TCTTCGGAGT GGCAGAGCGG GACCAAGGCA GTTACACGTG CATGGCCAGC 
ACCGAGCTGG ACCAGGACCT GGCAAAGGCC TACCTCACTG TTCTAGCTGA TCAGGCCACT 
CCGACTAACC GTTTGGCTGC CCTGCCCAAA GGGCGGCCAG ACCGACCCAG GGACCTGGAG 
CTCACTGACC TGGCGGAAAG GAGTGTGAGG CTGACCTGGA TCCCGGGGGA TGATAACAAC 
AGTCCCATCA CAGACTACGT TGTCCAGTTT GAAGAGGACC AGTTCCAGCC AGGAGTCTGG 
CATGACCACT CCAAGTTCCC AGGCAGTGTC AACTCAGCCG TCCTCCATCT GTCCCCGTAT 
GTCAACTATC AGTTCCGAGT CATCGCTGTC AACGAGGTTG GGAGCAGCCA CCCCAGCCTT 
CCATCCGAGC GGTACCGAAC AAGCGGGGCA CCCCCTGAAT CCAACCCCAG TGATGTGAAG 
GGCGAAGGGA CACGAAAAAA CAATATGGAG ATCACATGGA CGCCTATGAA TGCTACCTCC 
GCCTTTGGCC CCAACCTGCG CTACATTGTC AAGTGGCGAC GGAGAGAAAC CCGAGAGACT 
TGGAACAATG TCACCGTGTG GGGCTCTCGC TATGTGGTGG GGCAGACCCC TGTCTACGTA 
CCCTATGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AAGGCCCGGA GCCTGAAACC 
GTCATTGGGT ACTCGGGGGA AGATTATCCC AGGGCTGCAC CCACTGAAGT TAAAATCCGA 
GTCCTGAACA GCACAGCCAT CAGCCTTCAG TGGAACCGCG TCTACTCTGA CACGGTCCAG 
GGCCAGCTCA GAGAGTATCG AGCTTACTAC TGGAGGGAAA GCAGTTTGCT GAAGAACCTG 
TGGGTGTCTC AGAAGAGACA GCAGGCCAGC TTCCCTGGCG ACCGCCCCCG GGGCGTGGTG 
GGTCGCCTGT TCCCCTACAG TAACTACAAG CTGGAGATGG TTGTGGTCAA TGGGAGAGGT 
GATGGGCCTC GAAGTGAAAC CAAGGAATTC ACCACCCCGG AAGGAGTACC CAGTGCCCCC 
AGGCGTTTCA GAGTCCGACA GCCCAACCTG GAGACCATCA ACCTGGAATG GGATCACCCA 
GAGCACCCCA ACGGGATCCT GATTGGATAC ACGCTCAGAT ACGTGCCCTT TAATGGAACC 
AAACTGGGAA AGCAGATGGT GGAAAACTTC TCTCCCAATC AGACCAAGTT CTCGGTGCAG 
AGAGCAGACC CCGTGTCCCG TTACCGCTTC TCCCTCAGTG CCAGGACGCA GGTGGGCTCT 
GGAGAAGCAG CCACGGAGGA GTCCCCAACA CCTCCAAATG AAGCTACTCC AACTGCAGCT 
CCTCCCACAT TGCCCCCGAC TACCGTGGGT ACCACAGGCC TTGTGAGCAG TACTGATGCT 
ACTGCCCTTG CTGCCACCAG TGAAGCCACA ACAGTTCCCA TCATCCCAAC CGTCGTACCT 
ACCACCGTCG CCACCACCAT TGCCACAACT ACTACAACCA CTGCCGCCGC CACCACCACT 
ACCACCACGG AGAGTCCTCC CACTACCACC ACTGGGACTA AGATTCACGA AACCGCCCCC 
GACGAGCAGT CCATTTGGAA CGTCACGGTG CTCCCCAACA GTAAATGGGC CAACATCACC 
TGGAAGCACA ATTTCAGGCC TGGAACTGAC TTTGTGGTTG AGTACATCGA CAGCAACCAT 
ACGAAAAAAA CTGTCCCTGT TAAGGCCCAA GCCCAGCCCA TACAGCTGAC AGACCTCTTT 
CCCGGGATGA CGTACACGTT GCGGGTGTAT TCCCGGGACA ACGAGGGCAT CAGCAGTACC 
GTCATCACCT TTATGACCAG TACAGCTTAC ACCAACAACC AGACTGACAT CGCCACCCAG 
GGCTGGTTCA TCGGGCTCAT GTGTGCCATT GCCCTTCTGG TGCTGATCCT GCTTATCGTC 
TGCTTCATCA AGAGGAGTCG TGGCGGCAAG TACCCAGTGC GAGAAAAGAA GGATGTCCCC 
TTGGGCCCTG AAGACCCCAA AGAAGAAGAT GGTTCATTTG ACTACAGTGA CGAGGACAAC 
AAGCCCCTGC AGGGCAGCCA GACATCTCTG GACGGCACCA TCAAGCAGCA GGAGAGTGAC 
GACAGCCTAG TGGACTACGG CGAGGGTGGC GAGGGCCAGT TCAACGAAGA TGGCTCCTTT 
ATTGGCCAGT ACACTGTCAG AAAGGACAAG GAGGAGACCG AGGGCAATGA GAGCTCAGAG 
GCCACATCTC CAGTCAATGC CATCTATTCC CTGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454130.1
CDS	52..4410
Translation	MRRLAARAAAGDAGEVAAPGADIWDCAQGWSWSSWRLTDLCAIWEPGVFLPSAAAVNRSRASAGVAKSRPNEAEKLSTVWKAHEERRKASRARGLGSAEQGLALRMARQQAPPWVHVALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWLVNGDPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPETVIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVGRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPTPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAAATTTTTTESPPTTTTGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQTDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVRKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017598641.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurofascin (Nfasc), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598641.1

ORF Insert Sequence:

ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA 
TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA 
GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG 
AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG 
GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC 
CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT 
GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC 
CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG 
ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG 
TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG 
TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG 
GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT 
GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG 
GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA 
CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC 
ACCCGAGGAG TTGCGGAAAG AACACCTAGC TTCATGTATC CCCAGGGCAC GTCAAGCAGT 
CAGATGGTAC TGCGCGGCAT GGACCTGCTG CTGGAGTGCA TTGCCTCTGG GGTCCCAACA 
CCAGATATTG CATGGTACAA GAAAGGTGGG GACCTCCCAT CTGACAAGGC CAAGTTCGAG 
AATTTTAACA AGGCTCTGCG CATCACCAAT GTCTCTGAAG AGGACTCTGG GGAGTATTTC 
TGCCTGGCCT CCAACAAGAT GGGCAGCATC CGGCACACGA TCTCGGTGAG AGTAAAGGCT 
GCCCCATACT GGCTGGATGA GCCCAAGAAC TTGATCCTGG CTCCTGGTGA AGATGGGAGG 
CTGGTGTGTC GAGCCAATGG GAACCCGAAG CCGACCGTCC AGTGGCTGGT GAATGGAGAC 
CCTTTGCAAT CGGCACCACC CAACCCCAAC CGTGAGGTGG CCGGAGACAC TATCATCTTC 
CGGGACACTC AGATCAGCAG CAGGGCAGTG TACCAGTGTA ACACATCCAA CGAACATGGC 
TACCTGCTGG CCAATGCCTT CGTCAGCGTA TTAGATGTAC CCCCTCGGAT GCTGTCTCCC 
CGGAACCAGC TCATCAGGGT GATCCTTTAC AACCGGACGC GACTGGACTG TCCGTTCTTT 
GGGTCTCCCA TTCCAACACT CCGATGGTTT AAGAATGGGC AAGGAAGCAA CCTGGATGGT 
GGTAACTACC ACGTCTACGA AAATGGCAGC CTGGAAATCA AGATGATTCG CAAAGAGGAC 
CAAGGCATCT ACACCTGTGT GGCCACCAAC ATCCTGGGAA AAGCTGAAAA TCAAGTCCGC 
CTGGAGGTCA AAGACCCCAC CAGGATCTAC AGGATGCCTG AAGACCAGGT GGCCAAGAGG 
GGCACCACAG TGCAGCTGGA GTGCCGTGTG AAGCATGACC CCTCCTTGAA ACTCACAGTC 
TCCTGGCTGA AGGATGACGA GCCACTCTAC ATTGGAAACA GGATGAAAAA GGAAGATGAC 
TCCCTGACCA TCTTCGGAGT GGCAGAGCGG GACCAAGGCA GTTACACGTG CATGGCCAGC 
ACCGAGCTGG ACCAGGACCT GGCAAAGGCC TACCTCACTG TTCTAGCTGA TCAGGCCACT 
CCGACTAACC GTTTGGCTGC CCTGCCCAAA GGGCGGCCAG ACCGACCCAG GGACCTGGAG 
CTCACTGACC TGGCGGAAAG GAGTGTGAGG CTGACCTGGA TCCCGGGGGA TGATAACAAC 
AGTCCCATCA CAGACTACGT TGTCCAGTTT GAAGAGGACC AGTTCCAGCC AGGAGTCTGG 
CATGACCACT CCAAGTTCCC AGGCAGTGTC AACTCAGCCG TCCTCCATCT GTCCCCGTAT 
GTCAACTATC AGTTCCGAGT CATCGCTGTC AACGAGGTTG GGAGCAGCCA CCCCAGCCTT 
CCATCCGAGC GGTACCGAAC AAGCGGGGCA CCCCCTGAAT CCAACCCCAG TGATGTGAAG 
GGCGAAGGGA CACGAAAAAA CAATATGGAG ATCACATGGA CGCCTATGAA TGCTACCTCC 
GCCTTTGGCC CCAACCTGCG CTACATTGTC AAGTGGCGAC GGAGAGAAAC CCGAGAGACT 
TGGAACAATG TCACCGTGTG GGGCTCTCGC TATGTGGTGG GGCAGACCCC TGTCTACGTA 
CCCTATGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AAGGCCCGGA GCCTGAAACC 
GTCATTGGGT ACTCGGGGGA AGATTATCCC AGGGCTGCAC CCACTGAAGT TAAAATCCGA 
GTCCTGAACA GCACAGCCAT CAGCCTTCAG TGGAACCGCG TCTACTCTGA CACGGTCCAG 
GGCCAGCTCA GAGAGTATCG AGCTTACTAC TGGAGGGAAA GCAGTTTGCT GAAGAACCTG 
TGGGTGTCTC AGAAGAGACA GCAGGCCAGC TTCCCTGGCG ACCGCCCCCG GGGCGTGGTG 
GGTCGCCTGT TCCCCTACAG TAACTACAAG CTGGAGATGG TTGTGGTCAA TGGGAGAGGT 
GATGGGCCTC GAAGTGAAAC CAAGGAATTC ACCACCCCGG AAGGAGTACC CAGTGCCCCC 
AGGCGTTTCA GAGTCCGACA GCCCAACCTG GAGACCATCA ACCTGGAATG GGATCACCCA 
GAGCACCCCA ACGGGATCCT GATTGGATAC ACGCTCAGAT ACGTGCCCTT TAATGGAACC 
AAACTGGGAA AGCAGATGGT GGAAAACTTC TCTCCCAATC AGACCAAGTT CTCGGTGCAG 
AGAGCAGACC CCGTGTCCCG TTACCGCTTC TCCCTCAGTG CCAGGACGCA GGTGGGCTCT 
GGAGAAGCAG CCACGGAGGA GTCCCCAACA CCTCCAAATG AAGCTACTCC AACTGCAGCT 
CCTCCCACAT TGCCCCCGAC TACCGTGGGT ACCACAGGCC TTGTGAGCAG TACTGATGCT 
ACTGCCCTTG CTGCCACCAG TGAAGCCACA ACAGTTCCCA TCATCCCAAC CGTCGTACCT 
ACCACCGTCG CCACCACCAT TGCCACAACT ACTACAACCA CTGCCGCCGC CACCACCACT 
ACCACCACGG AGAGTCCTCC CACTACCACC ACTGGGACTA AGATTCACGA AACCGCCCCC 
GACGAGCAGT CCATTTGGAA CGTCACGGTG CTCCCCAACA GTAAATGGGC CAACATCACC 
TGGAAGCACA ATTTCAGGCC TGGAACTGAC TTTGTGGTTG AGTACATCGA CAGCAACCAT 
ACGAAAAAAA CTGTCCCTGT TAAGGCCCAA GCCCAGCCCA TACAGCTGAC AGACCTCTTT 
CCCGGGATGA CGTACACGTT GCGGGTGTAT TCCCGGGACA ACGAGGGCAT CAGCAGTACC 
GTCATCACCT TTATGACCAG TACAGCTTAC ACCAACAACC AGACTGACAT CGCCACCCAG 
GGCTGGTTCA TCGGGCTCAT GTGTGCCATT GCCCTTCTGG TGCTGATCCT GCTTATCGTC 
TGCTTCATCA AGAGGAGTCG TGGCGGCAAG TACCCAGTGC GAGAAAAGAA GGATGTCCCC 
TTGGGCCCTG AAGACCCCAA AGAAGAAGAT GGTTCATTTG ACTACAGTGA CGAGGACAAC 
AAGCCCCTGC AGGGCAGCCA GACATCTCTG GACGGCACCA TCAAGCAGCA GGAGAGTGAC 
GACAGCCTAG TGGACTACGG CGAGGGTGGC GAGGGCCAGT TCAACGAAGA TGGCTCCTTT 
ATTGGCCAGT ACACTGTCAG AAAGGACAAG GAGGAGACCG AGGGCAATGA GAGCTCAGAG 
GCCACATCTC CAGTCAATGC CATCTATTCC CTGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_017598642.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454131.1 Rattus norvegicus neurofascin (Nfasc), transcript variant X6, mRNA.

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CloneID	ORa57980
Clone ID Related Accession (Same CDS sequence)	XM_017598642.1
Accession Version	XM_017598642.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neurofascin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA 
TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA 
GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG 
AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG 
GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC 
CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCTGAC CCAACCCCCA 
ACGATCACCA AGCAGTCGGT GAAGGACCAC ATCGTGGACC CCCGAGATAA CATCCTGATT 
GAATGTGAAG CTAAAGGGAA CCCCGCCCCC AGTTTCCACT GGACTCGCAA CAGCAGGTTC 
TTCAACATTG CCAAGGACCC ACGGGTGTCC ATGAGGAGGA GGTCTGGGAC CTTGGTGATC 
GACTTCCGCA GTGGTGGGCG GCCTGAGGAG TACGAAGGGG AGTACCAGTG CTTTGCCCGG 
AACAAATTCG GCACAGCTCT TAGCAACCGC ATCCGCCTGC AGGTGTCCAA ATCTCCCCTG 
TGGCCCAAGG AAAACCTAGA CCCCGTCGTG GTTCAAGAGG GTGCCCCCTT AACCCTGCAG 
TGCAACCCCC CACCTGGCCT CCCATCCCCC GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AGGACAAGCG TGTCTCCCAG GGTCACAACG GGGACCTGTA CTTCTCCAAC 
GTCATGCTGC AGGACATGCA GACCGACTAC AGCTGCAATG CACGCTTCCA CTTCACCCAC 
ACCATTCAGC AGAAGAATCC CTTCACCCTC AAAGTCCTCA CCACCCGAGG AGTTGCGGAA 
AGAACACCTA GCTTCATGTA TCCCCAGGGC ACGTCAAGCA GTCAGATGGT ACTGCGCGGC 
ATGGACCTGC TGCTGGAGTG CATTGCCTCT GGGGTCCCAA CACCAGATAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTCG AGAATTTTAA CAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCCCCATA CTGGCTGGAT 
GAGCCCAAGA ACTTGATCCT GGCTCCTGGT GAAGATGGGA GGCTGGTGTG TCGAGCCAAT 
GGGAACCCGA AGCCGACCGT CCAGTGGCTG GTGAATGGAG ACCCTTTGCA ATCGGCACCA 
CCCAACCCCA ACCGTGAGGT GGCCGGAGAC ACTATCATCT TCCGGGACAC TCAGATCAGC 
AGCAGGGCAG TGTACCAGTG TAACACATCC AACGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TATTAGATGT ACCCCCTCGG ATGCTGTCTC CCCGGAACCA GCTCATCAGG 
GTGATCCTTT ACAACCGGAC GCGACTGGAC TGTCCGTTCT TTGGGTCTCC CATTCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GTGGTAACTA CCACGTCTAC 
GAAAATGGCA GCCTGGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG AAAAGCTGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC TGAAGACCAG GTGGCCAAGA GGGGCACCAC AGTGCAGCTG 
GAGTGCCGTG TGAAGCATGA CCCCTCCTTG AAACTCACAG TCTCCTGGCT GAAGGATGAC 
GAGCCACTCT ACATTGGAAA CAGGATGAAA AAGGAAGATG ACTCCCTGAC CATCTTCGGA 
GTGGCAGAGC GGGACCAAGG CAGTTACACG TGCATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCGACTAA CCGTTTGGCT 
GCCCTGCCCA AAGGGCGGCC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCGGAA 
AGGAGTGTGA GGCTGACCTG GATCCCGGGG GATGATAACA ACAGTCCCAT CACAGACTAC 
GTTGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGAGTCT GGCATGACCA CTCCAAGTTC 
CCAGGCAGTG TCAACTCAGC CGTCCTCCAT CTGTCCCCGT ATGTCAACTA TCAGTTCCGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACAAGCGGGG CACCCCCTGA ATCCAACCCC AGTGATGTGA AGGGCGAAGG GACACGAAAA 
AACAATATGG AGATCACATG GACGCCTATG AATGCTACCT CCGCCTTTGG CCCCAACCTG 
CGCTACATTG TCAAGTGGCG ACGGAGAGAA ACCCGAGAGA CTTGGAACAA TGTCACCGTG 
TGGGGCTCTC GCTATGTGGT GGGGCAGACC CCTGTCTACG TACCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCG GAGCCTGAAA CCGTCATTGG GTACTCGGGG 
GAAGATTATC CCAGGGCTGC ACCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC 
ATCAGCCTTC AGTGGAACCG CGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT 
CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA 
CAGCAGGCCA GCTTCCCTGG CGACCGCCCC CGGGGCGTGG TGGGTCGCCT GTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGAGAG GTGATGGGCC TCGAAGTGAA 
ACCAAGGAAT TCACCACCCC GGAAGGAGTA CCCAGTGCCC CCAGGCGTTT CAGAGTCCGA 
CAGCCCAACC TGGAGACCAT CAACCTGGAA TGGGATCACC CAGAGCACCC CAACGGGATC 
CTGATTGGAT ACACGCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCGGTGC AGAGAGCAGA CCCCGTGTCC 
CGTTACCGCT TCTCCCTCAG TGCCAGGACG CAGGTGGGCT CTGGAGAAGC AGCCACGGAG 
GAGTCCCCAA CACCTCCAAA TGAAGCTACT CCAACTGCAG CTCCTCCCAC ATTGCCCCCG 
ACTACCGTGG GTACCACAGG CCTTGTGAGC AGTACTGATG CTACTGCCCT TGCTGCCACC 
AGTGAAGCCA CAACAGTTCC CATCATCCCA ACCGTCGTAC CTACCACCGT CGCCACCACC 
ATTGCCACAA CTACTACAAC CACTGCCGCC GCCACCACCA CTACCACCAC GGAGAGTCCT 
CCCACTACCA CCACTGGGAC TAAGATTCAC GAAACCGCCC CCGACGAGCA GTCCATTTGG 
AACGTCACGG TGCTCCCCAA CAGTAAATGG GCCAACATCA CCTGGAAGCA CAATTTCAGG 
CCTGGAACTG ACTTTGTGGT TGAGTACATC GACAGCAACC ATACGAAAAA AACTGTCCCT 
GTTAAGGCCC AAGCCCAGCC CATACAGCTG ACAGACCTCT TTCCCGGGAT GACGTACACG 
TTGCGGGTGT ATTCCCGGGA CAACGAGGGC ATCAGCAGTA CCGTCATCAC CTTTATGACC 
AGTACAGCTT ACACCAACAA CCAGACTGAC ATCGCCACCC AGGGCTGGTT CATCGGGCTC 
ATGTGTGCCA TTGCCCTTCT GGTGCTGATC CTGCTTATCG TCTGCTTCAT CAAGAGGAGT 
CGTGGCGGCA AGTACCCAGT GCGAGAAAAG AAGGATGTCC CCTTGGGCCC TGAAGACCCC 
AAAGAAGAAG ATGGTTCATT TGACTACAGT GACGAGGACA ACAAGCCCCT GCAGGGCAGC 
CAGACATCTC TGGACGGCAC CATCAAGCAG CAGGAGAGTG ACGACAGCCT AGTGGACTAC 
GGCGAGGGTG GCGAGGGCCA GTTCAACGAA GATGGCTCCT TTATTGGCCA GTACACTGTC 
AGAAAGGACA AGGAGGAGAC CGAGGGCAAT GAGAGCTCAG AGGCCACATC TCCAGTCAAT 
GCCATCTATT CCCTGGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454131.1
CDS	52..4392
Translation	MRRLAARAAAGDAGEVAAPGADIWDCAQGWSWSSWRLTDLCAIWEPGVFLPSAAAVNRSRASAGVAKSRPNEAEKLSTVWKAHEERRKASRARGLGSAEQGLALRMARQQAPPWVHVALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWLVNGDPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPETVIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVGRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPTPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAAATTTTTTESPPTTTTGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQTDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVRKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017598642.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurofascin (Nfasc), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598642.1

ORF Insert Sequence:

ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA 
TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA 
GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG 
AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG 
GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC 
CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCTGAC CCAACCCCCA 
ACGATCACCA AGCAGTCGGT GAAGGACCAC ATCGTGGACC CCCGAGATAA CATCCTGATT 
GAATGTGAAG CTAAAGGGAA CCCCGCCCCC AGTTTCCACT GGACTCGCAA CAGCAGGTTC 
TTCAACATTG CCAAGGACCC ACGGGTGTCC ATGAGGAGGA GGTCTGGGAC CTTGGTGATC 
GACTTCCGCA GTGGTGGGCG GCCTGAGGAG TACGAAGGGG AGTACCAGTG CTTTGCCCGG 
AACAAATTCG GCACAGCTCT TAGCAACCGC ATCCGCCTGC AGGTGTCCAA ATCTCCCCTG 
TGGCCCAAGG AAAACCTAGA CCCCGTCGTG GTTCAAGAGG GTGCCCCCTT AACCCTGCAG 
TGCAACCCCC CACCTGGCCT CCCATCCCCC GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AGGACAAGCG TGTCTCCCAG GGTCACAACG GGGACCTGTA CTTCTCCAAC 
GTCATGCTGC AGGACATGCA GACCGACTAC AGCTGCAATG CACGCTTCCA CTTCACCCAC 
ACCATTCAGC AGAAGAATCC CTTCACCCTC AAAGTCCTCA CCACCCGAGG AGTTGCGGAA 
AGAACACCTA GCTTCATGTA TCCCCAGGGC ACGTCAAGCA GTCAGATGGT ACTGCGCGGC 
ATGGACCTGC TGCTGGAGTG CATTGCCTCT GGGGTCCCAA CACCAGATAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTCG AGAATTTTAA CAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCCCCATA CTGGCTGGAT 
GAGCCCAAGA ACTTGATCCT GGCTCCTGGT GAAGATGGGA GGCTGGTGTG TCGAGCCAAT 
GGGAACCCGA AGCCGACCGT CCAGTGGCTG GTGAATGGAG ACCCTTTGCA ATCGGCACCA 
CCCAACCCCA ACCGTGAGGT GGCCGGAGAC ACTATCATCT TCCGGGACAC TCAGATCAGC 
AGCAGGGCAG TGTACCAGTG TAACACATCC AACGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TATTAGATGT ACCCCCTCGG ATGCTGTCTC CCCGGAACCA GCTCATCAGG 
GTGATCCTTT ACAACCGGAC GCGACTGGAC TGTCCGTTCT TTGGGTCTCC CATTCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GTGGTAACTA CCACGTCTAC 
GAAAATGGCA GCCTGGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG AAAAGCTGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC TGAAGACCAG GTGGCCAAGA GGGGCACCAC AGTGCAGCTG 
GAGTGCCGTG TGAAGCATGA CCCCTCCTTG AAACTCACAG TCTCCTGGCT GAAGGATGAC 
GAGCCACTCT ACATTGGAAA CAGGATGAAA AAGGAAGATG ACTCCCTGAC CATCTTCGGA 
GTGGCAGAGC GGGACCAAGG CAGTTACACG TGCATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGCT GATCAGGCCA CTCCGACTAA CCGTTTGGCT 
GCCCTGCCCA AAGGGCGGCC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCGGAA 
AGGAGTGTGA GGCTGACCTG GATCCCGGGG GATGATAACA ACAGTCCCAT CACAGACTAC 
GTTGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGAGTCT GGCATGACCA CTCCAAGTTC 
CCAGGCAGTG TCAACTCAGC CGTCCTCCAT CTGTCCCCGT ATGTCAACTA TCAGTTCCGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACAAGCGGGG CACCCCCTGA ATCCAACCCC AGTGATGTGA AGGGCGAAGG GACACGAAAA 
AACAATATGG AGATCACATG GACGCCTATG AATGCTACCT CCGCCTTTGG CCCCAACCTG 
CGCTACATTG TCAAGTGGCG ACGGAGAGAA ACCCGAGAGA CTTGGAACAA TGTCACCGTG 
TGGGGCTCTC GCTATGTGGT GGGGCAGACC CCTGTCTACG TACCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCG GAGCCTGAAA CCGTCATTGG GTACTCGGGG 
GAAGATTATC CCAGGGCTGC ACCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC 
ATCAGCCTTC AGTGGAACCG CGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT 
CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA 
CAGCAGGCCA GCTTCCCTGG CGACCGCCCC CGGGGCGTGG TGGGTCGCCT GTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGAGAG GTGATGGGCC TCGAAGTGAA 
ACCAAGGAAT TCACCACCCC GGAAGGAGTA CCCAGTGCCC CCAGGCGTTT CAGAGTCCGA 
CAGCCCAACC TGGAGACCAT CAACCTGGAA TGGGATCACC CAGAGCACCC CAACGGGATC 
CTGATTGGAT ACACGCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCGGTGC AGAGAGCAGA CCCCGTGTCC 
CGTTACCGCT TCTCCCTCAG TGCCAGGACG CAGGTGGGCT CTGGAGAAGC AGCCACGGAG 
GAGTCCCCAA CACCTCCAAA TGAAGCTACT CCAACTGCAG CTCCTCCCAC ATTGCCCCCG 
ACTACCGTGG GTACCACAGG CCTTGTGAGC AGTACTGATG CTACTGCCCT TGCTGCCACC 
AGTGAAGCCA CAACAGTTCC CATCATCCCA ACCGTCGTAC CTACCACCGT CGCCACCACC 
ATTGCCACAA CTACTACAAC CACTGCCGCC GCCACCACCA CTACCACCAC GGAGAGTCCT 
CCCACTACCA CCACTGGGAC TAAGATTCAC GAAACCGCCC CCGACGAGCA GTCCATTTGG 
AACGTCACGG TGCTCCCCAA CAGTAAATGG GCCAACATCA CCTGGAAGCA CAATTTCAGG 
CCTGGAACTG ACTTTGTGGT TGAGTACATC GACAGCAACC ATACGAAAAA AACTGTCCCT 
GTTAAGGCCC AAGCCCAGCC CATACAGCTG ACAGACCTCT TTCCCGGGAT GACGTACACG 
TTGCGGGTGT ATTCCCGGGA CAACGAGGGC ATCAGCAGTA CCGTCATCAC CTTTATGACC 
AGTACAGCTT ACACCAACAA CCAGACTGAC ATCGCCACCC AGGGCTGGTT CATCGGGCTC 
ATGTGTGCCA TTGCCCTTCT GGTGCTGATC CTGCTTATCG TCTGCTTCAT CAAGAGGAGT 
CGTGGCGGCA AGTACCCAGT GCGAGAAAAG AAGGATGTCC CCTTGGGCCC TGAAGACCCC 
AAAGAAGAAG ATGGTTCATT TGACTACAGT GACGAGGACA ACAAGCCCCT GCAGGGCAGC 
CAGACATCTC TGGACGGCAC CATCAAGCAG CAGGAGAGTG ACGACAGCCT AGTGGACTAC 
GGCGAGGGTG GCGAGGGCCA GTTCAACGAA GATGGCTCCT TTATTGGCCA GTACACTGTC 
AGAAAGGACA AGGAGGAGAC CGAGGGCAAT GAGAGCTCAG AGGCCACATC TCCAGTCAAT 
GCCATCTATT CCCTGGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_017598643.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454132.1 Rattus norvegicus neurofascin (Nfasc), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ORa57981
Clone ID Related Accession (Same CDS sequence)	XM_017598643.1
Accession Version	XM_017598643.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neurofascin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA 
TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA 
GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG 
AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG 
GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC 
CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT 
GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC 
CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG 
ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG 
TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG 
TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG 
GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT 
GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG 
GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA 
CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC 
AACAACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATATATAG TGCCCGAGGA 
GTTGCGGAAA GAACACCTAG CTTCATGTAT CCCCAGGGCA CGTCAAGCAG TCAGATGGTA 
CTGCGCGGCA TGGACCTGCT GCTGGAGTGC ATTGCCTCTG GGGTCCCAAC ACCAGATATT 
GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTCGA GAATTTTAAC 
AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCCCCATAC 
TGGCTGGATG AGCCCAAGAA CTTGATCCTG GCTCCTGGTG AAGATGGGAG GCTGGTGTGT 
CGAGCCAATG GGAACCCGAA GCCGACCGTC CAGTGGCTGG TGAATGGAGA CCCTTTGCAA 
TCGGCACCAC CCAACCCCAA CCGTGAGGTG GCCGGAGACA CTATCATCTT CCGGGACACT 
CAGATCAGCA GCAGGGCAGT GTACCAGTGT AACACATCCA ACGAACATGG CTACCTGCTG 
GCCAATGCCT TCGTCAGCGT ATTAGATGTA CCCCCTCGGA TGCTGTCTCC CCGGAACCAG 
CTCATCAGGG TGATCCTTTA CAACCGGACG CGACTGGACT GTCCGTTCTT TGGGTCTCCC 
ATTCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG TGGTAACTAC 
CACGTCTACG AAAATGGCAG CCTGGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC 
TACACCTGTG TGGCCACCAA CATCCTGGGA AAAGCTGAAA ATCAAGTCCG CCTGGAGGTC 
AAAGACCCCA CCAGGATCTA CAGGATGCCT GAAGACCAGG TGGCCAAGAG GGGCACCACA 
GTGCAGCTGG AGTGCCGTGT GAAGCATGAC CCCTCCTTGA AACTCACAGT CTCCTGGCTG 
AAGGATGACG AGCCACTCTA CATTGGAAAC AGGATGAAAA AGGAAGATGA CTCCCTGACC 
ATCTTCGGAG TGGCAGAGCG GGACCAAGGC AGTTACACGT GCATGGCCAG CACCGAGCTG 
GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCGACTAAC 
CGTTTGGCTG CCCTGCCCAA AGGGCGGCCA GACCGACCCA GGGACCTGGA GCTCACTGAC 
CTGGCGGAAA GGAGTGTGAG GCTGACCTGG ATCCCGGGGG ATGATAACAA CAGTCCCATC 
ACAGACTACG TTGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGAGTCTG GCATGACCAC 
TCCAAGTTCC CAGGCAGTGT CAACTCAGCC GTCCTCCATC TGTCCCCGTA TGTCAACTAT 
CAGTTCCGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG 
CGGTACCGAA CAAGCGGGGC ACCCCCTGAA TCCAACCCCA GTGATGTGAA GGGCGAAGGG 
ACACGAAAAA ACAATATGGA GATCACATGG ACGCCTATGA ATGCTACCTC CGCCTTTGGC 
CCCAACCTGC GCTACATTGT CAAGTGGCGA CGGAGAGAAA CCCGAGAGAC TTGGAACAAT 
GTCACCGTGT GGGGCTCTCG CTATGTGGTG GGGCAGACCC CTGTCTACGT ACCCTATGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCGG AGCCTGAAAC CGTCATTGGG 
TACTCGGGGG AAGATTATCC CAGGGCTGCA CCCACTGAAG TTAAAATCCG AGTCCTGAAC 
AGCACAGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCTG ACACGGTCCA GGGCCAGCTC 
AGAGAGTATC GAGCTTACTA CTGGAGGGAA AGCAGTTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGAC AGCAGGCCAG CTTCCCTGGC GACCGCCCCC GGGGCGTGGT GGGTCGCCTG 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG TGATGGGCCT 
CGAAGTGAAA CCAAGGAATT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
AGAGTCCGAC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGATCACCC AGAGCACCCC 
AACGGGATCC TGATTGGATA CACGCTCAGA TACGTGCCCT TTAATGGAAC CAAACTGGGA 
AAGCAGATGG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCTCGGTGCA GAGAGCAGAC 
CCCGTGTCCC GTTACCGCTT CTCCCTCAGT GCCAGGACGC AGGTGGGCTC TGGAGAAGCA 
GCCACGGAGG AGTCCCCAAC ACCTCCAAAT GAAGCTACTC CAACTGCAGC TCCTCCCACA 
TTGCCCCCGA CTACCGTGGG TACCACAGGC CTTGTGAGCA GTACTGATGC TACTGCCCTT 
GCTGCCACCA GTGAAGCCAC AACAGTTCCC ATCATCCCAA CCGTCGTACC TACCACCGTC 
GCCACCACCA TTGCCACAAC TACTACAACC ACTGCCGCCG CCACCACCAC TACCACCACG 
GAGAGTCCTC CCACTACCAC CACTGGGACT AAGATTCACG AAACCGCTTA CACCAACAAC 
CAGACTGACA TCGCCACCCA GGGCTGGTTC ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG 
GTGCTGATCC TGCTTATCGT CTGCTTCATC AAGAGGAGTC GTGGCGGCAA GTACCCAGTG 
CGAGAAAAGA AGGATGTCCC CTTGGGCCCT GAAGACCCCA AAGAAGAAGA TGGTTCATTT 
GACTACAGTG ACGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACATCTCT GGACGGCACC 
ATCAAGCAGC AGGAGAGTGA CGACAGCCTA GTGGACTACG GCGAGGGTGG CGAGGGCCAG 
TTCAACGAAG ATGGCTCCTT TATTGGCCAG TACACTGTCA GAAAGGACAA GGAGGAGACC 
GAGGGCAATG AGAGCTCAGA GGCCACATCT CCAGTCAATG CCATCTATTC CCTGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454132.1
CDS	52..4191
Translation	MRRLAARAAAGDAGEVAAPGADIWDCAQGWSWSSWRLTDLCAIWEPGVFLPSAAAVNRSRASAGVAKSRPNEAEKLSTVWKAHEERRKASRARGLGSAEQGLALRMARQQAPPWVHVALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWLVNGDPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPETVIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVGRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPTPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAAATTTTTTESPPTTTTGTKIHETAYTNNQTDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVRKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017598643.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurofascin (Nfasc), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598643.1

ORF Insert Sequence:

ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA 
TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA 
GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG 
AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG 
GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC 
CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT 
GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC 
CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG 
ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG 
TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG 
TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG 
GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT 
GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG 
GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA 
CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC 
AACAACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATATATAG TGCCCGAGGA 
GTTGCGGAAA GAACACCTAG CTTCATGTAT CCCCAGGGCA CGTCAAGCAG TCAGATGGTA 
CTGCGCGGCA TGGACCTGCT GCTGGAGTGC ATTGCCTCTG GGGTCCCAAC ACCAGATATT 
GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTCGA GAATTTTAAC 
AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCCCCATAC 
TGGCTGGATG AGCCCAAGAA CTTGATCCTG GCTCCTGGTG AAGATGGGAG GCTGGTGTGT 
CGAGCCAATG GGAACCCGAA GCCGACCGTC CAGTGGCTGG TGAATGGAGA CCCTTTGCAA 
TCGGCACCAC CCAACCCCAA CCGTGAGGTG GCCGGAGACA CTATCATCTT CCGGGACACT 
CAGATCAGCA GCAGGGCAGT GTACCAGTGT AACACATCCA ACGAACATGG CTACCTGCTG 
GCCAATGCCT TCGTCAGCGT ATTAGATGTA CCCCCTCGGA TGCTGTCTCC CCGGAACCAG 
CTCATCAGGG TGATCCTTTA CAACCGGACG CGACTGGACT GTCCGTTCTT TGGGTCTCCC 
ATTCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG TGGTAACTAC 
CACGTCTACG AAAATGGCAG CCTGGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC 
TACACCTGTG TGGCCACCAA CATCCTGGGA AAAGCTGAAA ATCAAGTCCG CCTGGAGGTC 
AAAGACCCCA CCAGGATCTA CAGGATGCCT GAAGACCAGG TGGCCAAGAG GGGCACCACA 
GTGCAGCTGG AGTGCCGTGT GAAGCATGAC CCCTCCTTGA AACTCACAGT CTCCTGGCTG 
AAGGATGACG AGCCACTCTA CATTGGAAAC AGGATGAAAA AGGAAGATGA CTCCCTGACC 
ATCTTCGGAG TGGCAGAGCG GGACCAAGGC AGTTACACGT GCATGGCCAG CACCGAGCTG 
GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCGACTAAC 
CGTTTGGCTG CCCTGCCCAA AGGGCGGCCA GACCGACCCA GGGACCTGGA GCTCACTGAC 
CTGGCGGAAA GGAGTGTGAG GCTGACCTGG ATCCCGGGGG ATGATAACAA CAGTCCCATC 
ACAGACTACG TTGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGAGTCTG GCATGACCAC 
TCCAAGTTCC CAGGCAGTGT CAACTCAGCC GTCCTCCATC TGTCCCCGTA TGTCAACTAT 
CAGTTCCGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG 
CGGTACCGAA CAAGCGGGGC ACCCCCTGAA TCCAACCCCA GTGATGTGAA GGGCGAAGGG 
ACACGAAAAA ACAATATGGA GATCACATGG ACGCCTATGA ATGCTACCTC CGCCTTTGGC 
CCCAACCTGC GCTACATTGT CAAGTGGCGA CGGAGAGAAA CCCGAGAGAC TTGGAACAAT 
GTCACCGTGT GGGGCTCTCG CTATGTGGTG GGGCAGACCC CTGTCTACGT ACCCTATGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCGG AGCCTGAAAC CGTCATTGGG 
TACTCGGGGG AAGATTATCC CAGGGCTGCA CCCACTGAAG TTAAAATCCG AGTCCTGAAC 
AGCACAGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCTG ACACGGTCCA GGGCCAGCTC 
AGAGAGTATC GAGCTTACTA CTGGAGGGAA AGCAGTTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGAC AGCAGGCCAG CTTCCCTGGC GACCGCCCCC GGGGCGTGGT GGGTCGCCTG 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG TGATGGGCCT 
CGAAGTGAAA CCAAGGAATT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
AGAGTCCGAC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGATCACCC AGAGCACCCC 
AACGGGATCC TGATTGGATA CACGCTCAGA TACGTGCCCT TTAATGGAAC CAAACTGGGA 
AAGCAGATGG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCTCGGTGCA GAGAGCAGAC 
CCCGTGTCCC GTTACCGCTT CTCCCTCAGT GCCAGGACGC AGGTGGGCTC TGGAGAAGCA 
GCCACGGAGG AGTCCCCAAC ACCTCCAAAT GAAGCTACTC CAACTGCAGC TCCTCCCACA 
TTGCCCCCGA CTACCGTGGG TACCACAGGC CTTGTGAGCA GTACTGATGC TACTGCCCTT 
GCTGCCACCA GTGAAGCCAC AACAGTTCCC ATCATCCCAA CCGTCGTACC TACCACCGTC 
GCCACCACCA TTGCCACAAC TACTACAACC ACTGCCGCCG CCACCACCAC TACCACCACG 
GAGAGTCCTC CCACTACCAC CACTGGGACT AAGATTCACG AAACCGCTTA CACCAACAAC 
CAGACTGACA TCGCCACCCA GGGCTGGTTC ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG 
GTGCTGATCC TGCTTATCGT CTGCTTCATC AAGAGGAGTC GTGGCGGCAA GTACCCAGTG 
CGAGAAAAGA AGGATGTCCC CTTGGGCCCT GAAGACCCCA AAGAAGAAGA TGGTTCATTT 
GACTACAGTG ACGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACATCTCT GGACGGCACC 
ATCAAGCAGC AGGAGAGTGA CGACAGCCTA GTGGACTACG GCGAGGGTGG CGAGGGCCAG 
TTCAACGAAG ATGGCTCCTT TATTGGCCAG TACACTGTCA GAAAGGACAA GGAGGAGACC 
GAGGGCAATG AGAGCTCAGA GGCCACATCT CCAGTCAATG CCATCTATTC CCTGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_017598644.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454133.1 Rattus norvegicus neurofascin (Nfasc), transcript variant X8, mRNA.

Price & Availability↑

CloneID	ORa57982
Clone ID Related Accession (Same CDS sequence)	XM_017598644.1
Accession Version	XM_017598644.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neurofascin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA 
TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA 
GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG 
AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG 
GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC 
CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT 
GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC 
CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG 
ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG 
TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG 
TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG 
GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT 
GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG 
GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA 
CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC 
AACAACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATATATAG TGCCCGAGGA 
GTTGCGGAAA GAACACCTAG CTTCATGTAT CCCCAGGGCA CGTCAAGCAG TCAGATGGTA 
CTGCGCGGCA TGGACCTGCT GCTGGAGTGC ATTGCCTCTG GGGTCCCAAC ACCAGATATT 
GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTCGA GAATTTTAAC 
AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCCCCATAC 
TGGCTGGATG AGCCCAAGAA CTTGATCCTG GCTCCTGGTG AAGATGGGAG GCTGGTGTGT 
CGAGCCAATG GGAACCCGAA GCCGACCGTC CAGTGGCTGG TGAATGGAGA CCCTTTGCAA 
TCGGCACCAC CCAACCCCAA CCGTGAGGTG GCCGGAGACA CTATCATCTT CCGGGACACT 
CAGATCAGCA GCAGGGCAGT GTACCAGTGT AACACATCCA ACGAACATGG CTACCTGCTG 
GCCAATGCCT TCGTCAGCGT ATTAGATGTA CCCCCTCGGA TGCTGTCTCC CCGGAACCAG 
CTCATCAGGG TGATCCTTTA CAACCGGACG CGACTGGACT GTCCGTTCTT TGGGTCTCCC 
ATTCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG TGGTAACTAC 
CACGTCTACG AAAATGGCAG CCTGGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC 
TACACCTGTG TGGCCACCAA CATCCTGGGA AAAGCTGAAA ATCAAGTCCG CCTGGAGGTC 
AAAGACCCCA CCAGGATCTA CAGGATGCCT GAAGACCAGG TGGCCAAGAG GGGCACCACA 
GTGCAGCTGG AGTGCCGTGT GAAGCATGAC CCCTCCTTGA AACTCACAGT CTCCTGGCTG 
AAGGATGACG AGCCACTCTA CATTGGAAAC AGGATGAAAA AGGAAGATGA CTCCCTGACC 
ATCTTCGGAG TGGCAGAGCG GGACCAAGGC AGTTACACGT GCATGGCCAG CACCGAGCTG 
GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCGACTAAC 
CGTTTGGCTG CCCTGCCCAA AGGGCGGCCA GACCGACCCA GGGACCTGGA GCTCACTGAC 
CTGGCGGAAA GGAGTGTGAG GCTGACCTGG ATCCCGGGGG ATGATAACAA CAGTCCCATC 
ACAGACTACG TTGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGAGTCTG GCATGACCAC 
TCCAAGTTCC CAGGCAGTGT CAACTCAGCC GTCCTCCATC TGTCCCCGTA TGTCAACTAT 
CAGTTCCGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG 
CGGTACCGAA CAAGCGGGGC ACCCCCTGAA TCCAACCCCA GTGATGTGAA GGGCGAAGGG 
ACACGAAAAA ACAATATGGA GATCACATGG ACGCCTATGA ATGCTACCTC CGCCTTTGGC 
CCCAACCTGC GCTACATTGT CAAGTGGCGA CGGAGAGAAA CCCGAGAGAC TTGGAACAAT 
GTCACCGTGT GGGGCTCTCG CTATGTGGTG GGGCAGACCC CTGTCTACGT ACCCTATGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCGG AGCCTGAAAC CGTCATTGGG 
TACTCGGGGG AAGATTTACC CAGTGCCCCC AGGCGTTTCA GAGTCCGACA GCCCAACCTG 
GAGACCATCA ACCTGGAATG GGATCACCCA GAGCACCCCA ACGGGATCCT GATTGGATAC 
ACGCTCAGAT ACGTGCCCTT TAATGGAACC AAACTGGGAA AGCAGATGGT GGAAAACTTC 
TCTCCCAATC AGACCAAGTT CTCGGTGCAG AGAGCAGACC CCGTGTCCCG TTACCGCTTC 
TCCCTCAGTG CCAGGACGCA GGTGGGCTCT GGAGAAGCAG CCACGGAGGA GTCCCCAACA 
CCTCCAAATG AAGCTACTCC AACTGCAGCT CCTCCCACAT TGCCCCCGAC TACCGTGGGT 
ACCACAGGCC TTGTGAGCAG TACTGATGCT ACTGCCCTTG CTGCCACCAG TGAAGCCACA 
ACAGTTCCCA TCATCCCAAC CGTCGTACCT ACCACCGTCG CCACCACCAT TGCCACAACT 
ACTACAACCA CTGCCGCCGC CACCACCACT ACCACCACGG AGAGTCCTCC CACTACCACC 
ACTGGGACTA AGATTCACGA AACCGCCCCC GACGAGCAGT CCATTTGGAA CGTCACGGTG 
CTCCCCAACA GTAAATGGGC CAACATCACC TGGAAGCACA ATTTCAGGCC TGGAACTGAC 
TTTGTGGTTG AGTACATCGA CAGCAACCAT ACGAAAAAAA CTGTCCCTGT TAAGGCCCAA 
GCCCAGCCCA TACAGCTGAC AGACCTCTTT CCCGGGATGA CGTACACGTT GCGGGTGTAT 
TCCCGGGACA ACGAGGGCAT CAGCAGTACC GTCATCACCT TTATGACCAG TACAGCTTAC 
ACCAACAACC AGACTGACAT CGCCACCCAG GGCTGGTTCA TCGGGCTCAT GTGTGCCATT 
GCCCTTCTGG TGCTGATCCT GCTTATCGTC TGCTTCATCA AGAGGAGTCG TGGCGGCAAG 
TACCCAGTGC GAGAAAAGAA GGATGTCCCC TTGGGCCCTG AAGACCCCAA AGAAGAAGAT 
GGTTCATTTG ACTACAGTGA CGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACATCTCTG 
GACGGCACCA TCAAGCAGCA GGAGAGTGAC GACAGCCTAG TGGACTACGG CGAGGGTGGC 
GAGGGCCAGT TCAACGAAGA TGGCTCCTTT ATTGGCCAGT ACACTGTCAG AAAGGACAAG 
GAGGAGACCG AGGGCAATGA GAGCTCAGAG GCCACATCTC CAGTCAATGC CATCTATTCC 
CTGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454133.1
CDS	51..4139
Translation	MRRLAARAAAGDAGEVAAPGADIWDCAQGWSWSSWRLTDLCAIWEPGVFLPSAAAVNRSRASAGVAKSRPNEAEKLSTVWKAHEERRKASRARGLGSAEQGLALRMARQQAPPWVHVALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWLVNGDPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPETVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPTPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAAATTTTTTESPPTTTTGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQTDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVRKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017598644.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurofascin (Nfasc), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598644.1

ORF Insert Sequence:

ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA 
TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA 
GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG 
AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG 
GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC 
CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT 
GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC 
CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG 
ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG 
TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG 
TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG 
GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT 
GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG 
GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA 
CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC 
AACAACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATATATAG TGCCCGAGGA 
GTTGCGGAAA GAACACCTAG CTTCATGTAT CCCCAGGGCA CGTCAAGCAG TCAGATGGTA 
CTGCGCGGCA TGGACCTGCT GCTGGAGTGC ATTGCCTCTG GGGTCCCAAC ACCAGATATT 
GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTCGA GAATTTTAAC 
AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCCCCATAC 
TGGCTGGATG AGCCCAAGAA CTTGATCCTG GCTCCTGGTG AAGATGGGAG GCTGGTGTGT 
CGAGCCAATG GGAACCCGAA GCCGACCGTC CAGTGGCTGG TGAATGGAGA CCCTTTGCAA 
TCGGCACCAC CCAACCCCAA CCGTGAGGTG GCCGGAGACA CTATCATCTT CCGGGACACT 
CAGATCAGCA GCAGGGCAGT GTACCAGTGT AACACATCCA ACGAACATGG CTACCTGCTG 
GCCAATGCCT TCGTCAGCGT ATTAGATGTA CCCCCTCGGA TGCTGTCTCC CCGGAACCAG 
CTCATCAGGG TGATCCTTTA CAACCGGACG CGACTGGACT GTCCGTTCTT TGGGTCTCCC 
ATTCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG TGGTAACTAC 
CACGTCTACG AAAATGGCAG CCTGGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC 
TACACCTGTG TGGCCACCAA CATCCTGGGA AAAGCTGAAA ATCAAGTCCG CCTGGAGGTC 
AAAGACCCCA CCAGGATCTA CAGGATGCCT GAAGACCAGG TGGCCAAGAG GGGCACCACA 
GTGCAGCTGG AGTGCCGTGT GAAGCATGAC CCCTCCTTGA AACTCACAGT CTCCTGGCTG 
AAGGATGACG AGCCACTCTA CATTGGAAAC AGGATGAAAA AGGAAGATGA CTCCCTGACC 
ATCTTCGGAG TGGCAGAGCG GGACCAAGGC AGTTACACGT GCATGGCCAG CACCGAGCTG 
GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCGACTAAC 
CGTTTGGCTG CCCTGCCCAA AGGGCGGCCA GACCGACCCA GGGACCTGGA GCTCACTGAC 
CTGGCGGAAA GGAGTGTGAG GCTGACCTGG ATCCCGGGGG ATGATAACAA CAGTCCCATC 
ACAGACTACG TTGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGAGTCTG GCATGACCAC 
TCCAAGTTCC CAGGCAGTGT CAACTCAGCC GTCCTCCATC TGTCCCCGTA TGTCAACTAT 
CAGTTCCGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG 
CGGTACCGAA CAAGCGGGGC ACCCCCTGAA TCCAACCCCA GTGATGTGAA GGGCGAAGGG 
ACACGAAAAA ACAATATGGA GATCACATGG ACGCCTATGA ATGCTACCTC CGCCTTTGGC 
CCCAACCTGC GCTACATTGT CAAGTGGCGA CGGAGAGAAA CCCGAGAGAC TTGGAACAAT 
GTCACCGTGT GGGGCTCTCG CTATGTGGTG GGGCAGACCC CTGTCTACGT ACCCTATGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCGG AGCCTGAAAC CGTCATTGGG 
TACTCGGGGG AAGATTTACC CAGTGCCCCC AGGCGTTTCA GAGTCCGACA GCCCAACCTG 
GAGACCATCA ACCTGGAATG GGATCACCCA GAGCACCCCA ACGGGATCCT GATTGGATAC 
ACGCTCAGAT ACGTGCCCTT TAATGGAACC AAACTGGGAA AGCAGATGGT GGAAAACTTC 
TCTCCCAATC AGACCAAGTT CTCGGTGCAG AGAGCAGACC CCGTGTCCCG TTACCGCTTC 
TCCCTCAGTG CCAGGACGCA GGTGGGCTCT GGAGAAGCAG CCACGGAGGA GTCCCCAACA 
CCTCCAAATG AAGCTACTCC AACTGCAGCT CCTCCCACAT TGCCCCCGAC TACCGTGGGT 
ACCACAGGCC TTGTGAGCAG TACTGATGCT ACTGCCCTTG CTGCCACCAG TGAAGCCACA 
ACAGTTCCCA TCATCCCAAC CGTCGTACCT ACCACCGTCG CCACCACCAT TGCCACAACT 
ACTACAACCA CTGCCGCCGC CACCACCACT ACCACCACGG AGAGTCCTCC CACTACCACC 
ACTGGGACTA AGATTCACGA AACCGCCCCC GACGAGCAGT CCATTTGGAA CGTCACGGTG 
CTCCCCAACA GTAAATGGGC CAACATCACC TGGAAGCACA ATTTCAGGCC TGGAACTGAC 
TTTGTGGTTG AGTACATCGA CAGCAACCAT ACGAAAAAAA CTGTCCCTGT TAAGGCCCAA 
GCCCAGCCCA TACAGCTGAC AGACCTCTTT CCCGGGATGA CGTACACGTT GCGGGTGTAT 
TCCCGGGACA ACGAGGGCAT CAGCAGTACC GTCATCACCT TTATGACCAG TACAGCTTAC 
ACCAACAACC AGACTGACAT CGCCACCCAG GGCTGGTTCA TCGGGCTCAT GTGTGCCATT 
GCCCTTCTGG TGCTGATCCT GCTTATCGTC TGCTTCATCA AGAGGAGTCG TGGCGGCAAG 
TACCCAGTGC GAGAAAAGAA GGATGTCCCC TTGGGCCCTG AAGACCCCAA AGAAGAAGAT 
GGTTCATTTG ACTACAGTGA CGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACATCTCTG 
GACGGCACCA TCAAGCAGCA GGAGAGTGAC GACAGCCTAG TGGACTACGG CGAGGGTGGC 
GAGGGCCAGT TCAACGAAGA TGGCTCCTTT ATTGGCCAGT ACACTGTCAG AAAGGACAAG 
GAGGAGACCG AGGGCAATGA GAGCTCAGAG GCCACATCTC CAGTCAATGC CATCTATTCC 
CTGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_017598645.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454134.1 Rattus norvegicus neurofascin (Nfasc), transcript variant X10, mRNA.

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CloneID	ORa57983
Clone ID Related Accession (Same CDS sequence)	XM_017598645.1 , XM_017598646.1
Accession Version	XM_017598645.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neurofascin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA 
TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA 
GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG 
AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG 
GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC 
CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT 
GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC 
CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG 
ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG 
TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG 
TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG 
GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT 
GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG 
GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA 
CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC 
AACAACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATATATAG TGCCCGAGGA 
GTTGCGGAAA GAACACCTAG CTTCATGTAT CCCCAGGGCA CGTCAAGCAG TCAGATGGTA 
CTGCGCGGCA TGGACCTGCT GCTGGAGTGC ATTGCCTCTG GGGTCCCAAC ACCAGATATT 
GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTCGA GAATTTTAAC 
AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCCCCATAC 
TGGCTGGATG AGCCCAAGAA CTTGATCCTG GCTCCTGGTG AAGATGGGAG GCTGGTGTGT 
CGAGCCAATG GGAACCCGAA GCCGACCGTC CAGTGGCTGG TGAATGGAGA CCCTTTGCAA 
TCGGCACCAC CCAACCCCAA CCGTGAGGTG GCCGGAGACA CTATCATCTT CCGGGACACT 
CAGATCAGCA GCAGGGCAGT GTACCAGTGT AACACATCCA ACGAACATGG CTACCTGCTG 
GCCAATGCCT TCGTCAGCGT ATTAGATGTA CCCCCTCGGA TGCTGTCTCC CCGGAACCAG 
CTCATCAGGG TGATCCTTTA CAACCGGACG CGACTGGACT GTCCGTTCTT TGGGTCTCCC 
ATTCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG TGGTAACTAC 
CACGTCTACG AAAATGGCAG CCTGGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC 
TACACCTGTG TGGCCACCAA CATCCTGGGA AAAGCTGAAA ATCAAGTCCG CCTGGAGGTC 
AAAGACCCCA CCAGGATCTA CAGGATGCCT GAAGACCAGG TGGCCAAGAG GGGCACCACA 
GTGCAGCTGG AGTGCCGTGT GAAGCATGAC CCCTCCTTGA AACTCACAGT CTCCTGGCTG 
AAGGATGACG AGCCACTCTA CATTGGAAAC AGGATGAAAA AGGAAGATGA CTCCCTGACC 
ATCTTCGGAG TGGCAGAGCG GGACCAAGGC AGTTACACGT GCATGGCCAG CACCGAGCTG 
GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCGACTAAC 
CGTTTGGCTG CCCTGCCCAA AGGGCGGCCA GACCGACCCA GGGACCTGGA GCTCACTGAC 
CTGGCGGAAA GGAGTGTGAG GCTGACCTGG ATCCCGGGGG ATGATAACAA CAGTCCCATC 
ACAGACTACG TTGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGAGTCTG GCATGACCAC 
TCCAAGTTCC CAGGCAGTGT CAACTCAGCC GTCCTCCATC TGTCCCCGTA TGTCAACTAT 
CAGTTCCGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG 
CGGTACCGAA CAAGCGGGGC ACCCCCTGAA TCCAACCCCA GTGATGTGAA GGGCGAAGGG 
ACACGAAAAA ACAATATGGA GATCACATGG ACGCCTATGA ATGCTACCTC CGCCTTTGGC 
CCCAACCTGC GCTACATTGT CAAGTGGCGA CGGAGAGAAA CCCGAGAGAC TTGGAACAAT 
GTCACCGTGT GGGGCTCTCG CTATGTGGTG GGGCAGACCC CTGTCTACGT ACCCTATGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCGG AGCCTGAAAC CGTCATTGGG 
TACTCGGGGG AAGATTATCC CAGGGCTGCA CCCACTGAAG TTAAAATCCG AGTCCTGAAC 
AGCACAGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCTG ACACGGTCCA GGGCCAGCTC 
AGAGAGTATC GAGCTTACTA CTGGAGGGAA AGCAGTTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGAC AGCAGGCCAG CTTCCCTGGC GACCGCCCCC GGGGCGTGGT GGGTCGCCTG 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG TGATGGGCCT 
CGAAGTGAAA CCAAGGAATT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
AGAGTCCGAC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGATCACCC AGAGCACCCC 
AACGGGATCC TGATTGGATA CACGCTCAGA TACGTGCCCT TTAATGGAAC CAAACTGGGA 
AAGCAGATGG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCTCGGTGCA GAGAGCAGAC 
CCCGTGTCCC GTTACCGCTT CTCCCTCAGT GCCAGGACGC AGGTGGGCTC TGGAGAAGCA 
GCCACGGAGG AGTCCCCAAC ACCTCCAAAT GAAGCTACTC CAACTGCAGC TTACACCAAC 
AACCAGACTG ACATCGCCAC CCAGGGCTGG TTCATCGGGC TCATGTGTGC CATTGCCCTT 
CTGGTGCTGA TCCTGCTTAT CGTCTGCTTC ATCAAGAGGA GTCGTGGCGG CAAGTACCCA 
GTGCGAGAAA AGAAGGATGT CCCCTTGGGC CCTGAAGACC CCAAAGAAGA AGATGGTTCA 
TTTGACTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACATC TCTGGACGGC 
ACCATCAAGC AGCAGGAGAG TGACGACAGC CTAGTGGACT ACGGCGAGGG TGGCGAGGGC 
CAGTTCAACG AAGATGGCTC CTTTATTGGC CAGTACACTG TCAGAAAGGA CAAGGAGGAG 
ACCGAGGGCA ATGAGAGCTC AGAGGCCACA TCTCCAGTCA ATGCCATCTA TTCCCTGGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454134.1
CDS	51..3953
Translation	MRRLAARAAAGDAGEVAAPGADIWDCAQGWSWSSWRLTDLCAIWEPGVFLPSAAAVNRSRASAGVAKSRPNEAEKLSTVWKAHEERRKASRARGLGSAEQGLALRMARQQAPPWVHVALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWLVNGDPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPETVIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVGRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPTPPNEATPTAAYTNNQTDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVRKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017598645.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurofascin (Nfasc), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598645.1

ORF Insert Sequence:

ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA 
TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA 
GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG 
AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG 
GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC 
CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT 
GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC 
CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG 
ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG 
TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG 
TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG 
GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT 
GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG 
GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA 
CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC 
AACAACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATATATAG TGCCCGAGGA 
GTTGCGGAAA GAACACCTAG CTTCATGTAT CCCCAGGGCA CGTCAAGCAG TCAGATGGTA 
CTGCGCGGCA TGGACCTGCT GCTGGAGTGC ATTGCCTCTG GGGTCCCAAC ACCAGATATT 
GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTCGA GAATTTTAAC 
AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCCCCATAC 
TGGCTGGATG AGCCCAAGAA CTTGATCCTG GCTCCTGGTG AAGATGGGAG GCTGGTGTGT 
CGAGCCAATG GGAACCCGAA GCCGACCGTC CAGTGGCTGG TGAATGGAGA CCCTTTGCAA 
TCGGCACCAC CCAACCCCAA CCGTGAGGTG GCCGGAGACA CTATCATCTT CCGGGACACT 
CAGATCAGCA GCAGGGCAGT GTACCAGTGT AACACATCCA ACGAACATGG CTACCTGCTG 
GCCAATGCCT TCGTCAGCGT ATTAGATGTA CCCCCTCGGA TGCTGTCTCC CCGGAACCAG 
CTCATCAGGG TGATCCTTTA CAACCGGACG CGACTGGACT GTCCGTTCTT TGGGTCTCCC 
ATTCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG TGGTAACTAC 
CACGTCTACG AAAATGGCAG CCTGGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC 
TACACCTGTG TGGCCACCAA CATCCTGGGA AAAGCTGAAA ATCAAGTCCG CCTGGAGGTC 
AAAGACCCCA CCAGGATCTA CAGGATGCCT GAAGACCAGG TGGCCAAGAG GGGCACCACA 
GTGCAGCTGG AGTGCCGTGT GAAGCATGAC CCCTCCTTGA AACTCACAGT CTCCTGGCTG 
AAGGATGACG AGCCACTCTA CATTGGAAAC AGGATGAAAA AGGAAGATGA CTCCCTGACC 
ATCTTCGGAG TGGCAGAGCG GGACCAAGGC AGTTACACGT GCATGGCCAG CACCGAGCTG 
GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCGACTAAC 
CGTTTGGCTG CCCTGCCCAA AGGGCGGCCA GACCGACCCA GGGACCTGGA GCTCACTGAC 
CTGGCGGAAA GGAGTGTGAG GCTGACCTGG ATCCCGGGGG ATGATAACAA CAGTCCCATC 
ACAGACTACG TTGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGAGTCTG GCATGACCAC 
TCCAAGTTCC CAGGCAGTGT CAACTCAGCC GTCCTCCATC TGTCCCCGTA TGTCAACTAT 
CAGTTCCGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG 
CGGTACCGAA CAAGCGGGGC ACCCCCTGAA TCCAACCCCA GTGATGTGAA GGGCGAAGGG 
ACACGAAAAA ACAATATGGA GATCACATGG ACGCCTATGA ATGCTACCTC CGCCTTTGGC 
CCCAACCTGC GCTACATTGT CAAGTGGCGA CGGAGAGAAA CCCGAGAGAC TTGGAACAAT 
GTCACCGTGT GGGGCTCTCG CTATGTGGTG GGGCAGACCC CTGTCTACGT ACCCTATGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCGG AGCCTGAAAC CGTCATTGGG 
TACTCGGGGG AAGATTATCC CAGGGCTGCA CCCACTGAAG TTAAAATCCG AGTCCTGAAC 
AGCACAGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCTG ACACGGTCCA GGGCCAGCTC 
AGAGAGTATC GAGCTTACTA CTGGAGGGAA AGCAGTTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGAC AGCAGGCCAG CTTCCCTGGC GACCGCCCCC GGGGCGTGGT GGGTCGCCTG 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG TGATGGGCCT 
CGAAGTGAAA CCAAGGAATT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
AGAGTCCGAC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGATCACCC AGAGCACCCC 
AACGGGATCC TGATTGGATA CACGCTCAGA TACGTGCCCT TTAATGGAAC CAAACTGGGA 
AAGCAGATGG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCTCGGTGCA GAGAGCAGAC 
CCCGTGTCCC GTTACCGCTT CTCCCTCAGT GCCAGGACGC AGGTGGGCTC TGGAGAAGCA 
GCCACGGAGG AGTCCCCAAC ACCTCCAAAT GAAGCTACTC CAACTGCAGC TTACACCAAC 
AACCAGACTG ACATCGCCAC CCAGGGCTGG TTCATCGGGC TCATGTGTGC CATTGCCCTT 
CTGGTGCTGA TCCTGCTTAT CGTCTGCTTC ATCAAGAGGA GTCGTGGCGG CAAGTACCCA 
GTGCGAGAAA AGAAGGATGT CCCCTTGGGC CCTGAAGACC CCAAAGAAGA AGATGGTTCA 
TTTGACTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACATC TCTGGACGGC 
ACCATCAAGC AGCAGGAGAG TGACGACAGC CTAGTGGACT ACGGCGAGGG TGGCGAGGGC 
CAGTTCAACG AAGATGGCTC CTTTATTGGC CAGTACACTG TCAGAAAGGA CAAGGAGGAG 
ACCGAGGGCA ATGAGAGCTC AGAGGCCACA TCTCCAGTCA ATGCCATCTA TTCCCTGGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_017598646.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454135.1 Rattus norvegicus neurofascin (Nfasc), transcript variant X11, mRNA.

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CloneID	ORa57983
Clone ID Related Accession (Same CDS sequence)	XM_017598645.1 , XM_017598646.1
Accession Version	XM_017598646.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neurofascin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA 
TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA 
GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG 
AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG 
GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC 
CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT 
GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC 
CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG 
ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG 
TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG 
TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG 
GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT 
GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG 
GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA 
CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC 
AACAACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATATATAG TGCCCGAGGA 
GTTGCGGAAA GAACACCTAG CTTCATGTAT CCCCAGGGCA CGTCAAGCAG TCAGATGGTA 
CTGCGCGGCA TGGACCTGCT GCTGGAGTGC ATTGCCTCTG GGGTCCCAAC ACCAGATATT 
GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTCGA GAATTTTAAC 
AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCCCCATAC 
TGGCTGGATG AGCCCAAGAA CTTGATCCTG GCTCCTGGTG AAGATGGGAG GCTGGTGTGT 
CGAGCCAATG GGAACCCGAA GCCGACCGTC CAGTGGCTGG TGAATGGAGA CCCTTTGCAA 
TCGGCACCAC CCAACCCCAA CCGTGAGGTG GCCGGAGACA CTATCATCTT CCGGGACACT 
CAGATCAGCA GCAGGGCAGT GTACCAGTGT AACACATCCA ACGAACATGG CTACCTGCTG 
GCCAATGCCT TCGTCAGCGT ATTAGATGTA CCCCCTCGGA TGCTGTCTCC CCGGAACCAG 
CTCATCAGGG TGATCCTTTA CAACCGGACG CGACTGGACT GTCCGTTCTT TGGGTCTCCC 
ATTCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG TGGTAACTAC 
CACGTCTACG AAAATGGCAG CCTGGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC 
TACACCTGTG TGGCCACCAA CATCCTGGGA AAAGCTGAAA ATCAAGTCCG CCTGGAGGTC 
AAAGACCCCA CCAGGATCTA CAGGATGCCT GAAGACCAGG TGGCCAAGAG GGGCACCACA 
GTGCAGCTGG AGTGCCGTGT GAAGCATGAC CCCTCCTTGA AACTCACAGT CTCCTGGCTG 
AAGGATGACG AGCCACTCTA CATTGGAAAC AGGATGAAAA AGGAAGATGA CTCCCTGACC 
ATCTTCGGAG TGGCAGAGCG GGACCAAGGC AGTTACACGT GCATGGCCAG CACCGAGCTG 
GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCGACTAAC 
CGTTTGGCTG CCCTGCCCAA AGGGCGGCCA GACCGACCCA GGGACCTGGA GCTCACTGAC 
CTGGCGGAAA GGAGTGTGAG GCTGACCTGG ATCCCGGGGG ATGATAACAA CAGTCCCATC 
ACAGACTACG TTGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGAGTCTG GCATGACCAC 
TCCAAGTTCC CAGGCAGTGT CAACTCAGCC GTCCTCCATC TGTCCCCGTA TGTCAACTAT 
CAGTTCCGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG 
CGGTACCGAA CAAGCGGGGC ACCCCCTGAA TCCAACCCCA GTGATGTGAA GGGCGAAGGG 
ACACGAAAAA ACAATATGGA GATCACATGG ACGCCTATGA ATGCTACCTC CGCCTTTGGC 
CCCAACCTGC GCTACATTGT CAAGTGGCGA CGGAGAGAAA CCCGAGAGAC TTGGAACAAT 
GTCACCGTGT GGGGCTCTCG CTATGTGGTG GGGCAGACCC CTGTCTACGT ACCCTATGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCGG AGCCTGAAAC CGTCATTGGG 
TACTCGGGGG AAGATTATCC CAGGGCTGCA CCCACTGAAG TTAAAATCCG AGTCCTGAAC 
AGCACAGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCTG ACACGGTCCA GGGCCAGCTC 
AGAGAGTATC GAGCTTACTA CTGGAGGGAA AGCAGTTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGAC AGCAGGCCAG CTTCCCTGGC GACCGCCCCC GGGGCGTGGT GGGTCGCCTG 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG TGATGGGCCT 
CGAAGTGAAA CCAAGGAATT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
AGAGTCCGAC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGATCACCC AGAGCACCCC 
AACGGGATCC TGATTGGATA CACGCTCAGA TACGTGCCCT TTAATGGAAC CAAACTGGGA 
AAGCAGATGG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCTCGGTGCA GAGAGCAGAC 
CCCGTGTCCC GTTACCGCTT CTCCCTCAGT GCCAGGACGC AGGTGGGCTC TGGAGAAGCA 
GCCACGGAGG AGTCCCCAAC ACCTCCAAAT GAAGCTACTC CAACTGCAGC TTACACCAAC 
AACCAGACTG ACATCGCCAC CCAGGGCTGG TTCATCGGGC TCATGTGTGC CATTGCCCTT 
CTGGTGCTGA TCCTGCTTAT CGTCTGCTTC ATCAAGAGGA GTCGTGGCGG CAAGTACCCA 
GTGCGAGAAA AGAAGGATGT CCCCTTGGGC CCTGAAGACC CCAAAGAAGA AGATGGTTCA 
TTTGACTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACATC TCTGGACGGC 
ACCATCAAGC AGCAGGAGAG TGACGACAGC CTAGTGGACT ACGGCGAGGG TGGCGAGGGC 
CAGTTCAACG AAGATGGCTC CTTTATTGGC CAGTACACTG TCAGAAAGGA CAAGGAGGAG 
ACCGAGGGCA ATGAGAGCTC AGAGGCCACA TCTCCAGTCA ATGCCATCTA TTCCCTGGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454135.1
CDS	51..3953
Translation	MRRLAARAAAGDAGEVAAPGADIWDCAQGWSWSSWRLTDLCAIWEPGVFLPSAAAVNRSRASAGVAKSRPNEAEKLSTVWKAHEERRKASRARGLGSAEQGLALRMARQQAPPWVHVALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWLVNGDPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPETVIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVGRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPTPPNEATPTAAYTNNQTDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVRKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017598646.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurofascin (Nfasc), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598646.1

ORF Insert Sequence:

ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA 
TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA 
GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG 
AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG 
GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC 
CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT 
GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC 
CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG 
ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG 
TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG 
TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG 
GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT 
GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG 
GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA 
CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC 
AACAACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATATATAG TGCCCGAGGA 
GTTGCGGAAA GAACACCTAG CTTCATGTAT CCCCAGGGCA CGTCAAGCAG TCAGATGGTA 
CTGCGCGGCA TGGACCTGCT GCTGGAGTGC ATTGCCTCTG GGGTCCCAAC ACCAGATATT 
GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTCGA GAATTTTAAC 
AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCCCCATAC 
TGGCTGGATG AGCCCAAGAA CTTGATCCTG GCTCCTGGTG AAGATGGGAG GCTGGTGTGT 
CGAGCCAATG GGAACCCGAA GCCGACCGTC CAGTGGCTGG TGAATGGAGA CCCTTTGCAA 
TCGGCACCAC CCAACCCCAA CCGTGAGGTG GCCGGAGACA CTATCATCTT CCGGGACACT 
CAGATCAGCA GCAGGGCAGT GTACCAGTGT AACACATCCA ACGAACATGG CTACCTGCTG 
GCCAATGCCT TCGTCAGCGT ATTAGATGTA CCCCCTCGGA TGCTGTCTCC CCGGAACCAG 
CTCATCAGGG TGATCCTTTA CAACCGGACG CGACTGGACT GTCCGTTCTT TGGGTCTCCC 
ATTCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG TGGTAACTAC 
CACGTCTACG AAAATGGCAG CCTGGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC 
TACACCTGTG TGGCCACCAA CATCCTGGGA AAAGCTGAAA ATCAAGTCCG CCTGGAGGTC 
AAAGACCCCA CCAGGATCTA CAGGATGCCT GAAGACCAGG TGGCCAAGAG GGGCACCACA 
GTGCAGCTGG AGTGCCGTGT GAAGCATGAC CCCTCCTTGA AACTCACAGT CTCCTGGCTG 
AAGGATGACG AGCCACTCTA CATTGGAAAC AGGATGAAAA AGGAAGATGA CTCCCTGACC 
ATCTTCGGAG TGGCAGAGCG GGACCAAGGC AGTTACACGT GCATGGCCAG CACCGAGCTG 
GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCGACTAAC 
CGTTTGGCTG CCCTGCCCAA AGGGCGGCCA GACCGACCCA GGGACCTGGA GCTCACTGAC 
CTGGCGGAAA GGAGTGTGAG GCTGACCTGG ATCCCGGGGG ATGATAACAA CAGTCCCATC 
ACAGACTACG TTGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGAGTCTG GCATGACCAC 
TCCAAGTTCC CAGGCAGTGT CAACTCAGCC GTCCTCCATC TGTCCCCGTA TGTCAACTAT 
CAGTTCCGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG 
CGGTACCGAA CAAGCGGGGC ACCCCCTGAA TCCAACCCCA GTGATGTGAA GGGCGAAGGG 
ACACGAAAAA ACAATATGGA GATCACATGG ACGCCTATGA ATGCTACCTC CGCCTTTGGC 
CCCAACCTGC GCTACATTGT CAAGTGGCGA CGGAGAGAAA CCCGAGAGAC TTGGAACAAT 
GTCACCGTGT GGGGCTCTCG CTATGTGGTG GGGCAGACCC CTGTCTACGT ACCCTATGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCGG AGCCTGAAAC CGTCATTGGG 
TACTCGGGGG AAGATTATCC CAGGGCTGCA CCCACTGAAG TTAAAATCCG AGTCCTGAAC 
AGCACAGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCTG ACACGGTCCA GGGCCAGCTC 
AGAGAGTATC GAGCTTACTA CTGGAGGGAA AGCAGTTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGAC AGCAGGCCAG CTTCCCTGGC GACCGCCCCC GGGGCGTGGT GGGTCGCCTG 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG TGATGGGCCT 
CGAAGTGAAA CCAAGGAATT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
AGAGTCCGAC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGATCACCC AGAGCACCCC 
AACGGGATCC TGATTGGATA CACGCTCAGA TACGTGCCCT TTAATGGAAC CAAACTGGGA 
AAGCAGATGG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCTCGGTGCA GAGAGCAGAC 
CCCGTGTCCC GTTACCGCTT CTCCCTCAGT GCCAGGACGC AGGTGGGCTC TGGAGAAGCA 
GCCACGGAGG AGTCCCCAAC ACCTCCAAAT GAAGCTACTC CAACTGCAGC TTACACCAAC 
AACCAGACTG ACATCGCCAC CCAGGGCTGG TTCATCGGGC TCATGTGTGC CATTGCCCTT 
CTGGTGCTGA TCCTGCTTAT CGTCTGCTTC ATCAAGAGGA GTCGTGGCGG CAAGTACCCA 
GTGCGAGAAA AGAAGGATGT CCCCTTGGGC CCTGAAGACC CCAAAGAAGA AGATGGTTCA 
TTTGACTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACATC TCTGGACGGC 
ACCATCAAGC AGCAGGAGAG TGACGACAGC CTAGTGGACT ACGGCGAGGG TGGCGAGGGC 
CAGTTCAACG AAGATGGCTC CTTTATTGGC CAGTACACTG TCAGAAAGGA CAAGGAGGAG 
ACCGAGGGCA ATGAGAGCTC AGAGGCCACA TCTCCAGTCA ATGCCATCTA TTCCCTGGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_017598647.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454136.1 Rattus norvegicus neurofascin (Nfasc), transcript variant X12, mRNA.

Price & Availability↑

CloneID	ORa57984
Clone ID Related Accession (Same CDS sequence)	XM_017598647.1
Accession Version	XM_017598647.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neurofascin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA 
TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA 
GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG 
AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG 
GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC 
CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT 
GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC 
CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG 
ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG 
TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG 
TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG 
GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT 
GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG 
GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA 
CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC 
AACAACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATATATAG TGCCCGAGGA 
GTTGCGGAAA GAACACCTAG CTTCATGTAT CCCCAGGGCA CGTCAAGCAG TCAGATGGTA 
CTGCGCGGCA TGGACCTGCT GCTGGAGTGC ATTGCCTCTG GGGTCCCAAC ACCAGATATT 
GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTCGA GAATTTTAAC 
AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCCCCATAC 
TGGCTGGATG AGCCCAAGAA CTTGATCCTG GCTCCTGGTG AAGATGGGAG GCTGGTGTGT 
CGAGCCAATG GGAACCCGAA GCCGACCGTC CAGTGGCTGG TGAATGGAGA CCCTTTGCAA 
TCGGCACCAC CCAACCCCAA CCGTGAGGTG GCCGGAGACA CTATCATCTT CCGGGACACT 
CAGATCAGCA GCAGGGCAGT GTACCAGTGT AACACATCCA ACGAACATGG CTACCTGCTG 
GCCAATGCCT TCGTCAGCGT ATTAGATGTA CCCCCTCGGA TGCTGTCTCC CCGGAACCAG 
CTCATCAGGG TGATCCTTTA CAACCGGACG CGACTGGACT GTCCGTTCTT TGGGTCTCCC 
ATTCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG TGGTAACTAC 
CACGTCTACG AAAATGGCAG CCTGGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC 
TACACCTGTG TGGCCACCAA CATCCTGGGA AAAGCTGAAA ATCAAGTCCG CCTGGAGGTC 
AAAGACCCCA CCAGGATCTA CAGGATGCCT GAAGACCAGG TGGCCAAGAG GGGCACCACA 
GTGCAGCTGG AGTGCCGTGT GAAGCATGAC CCCTCCTTGA AACTCACAGT CTCCTGGCTG 
AAGGATGACG AGCCACTCTA CATTGGAAAC AGGATGAAAA AGGAAGATGA CTCCCTGACC 
ATCTTCGGAG TGGCAGAGCG GGACCAAGGC AGTTACACGT GCATGGCCAG CACCGAGCTG 
GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCGACTAAC 
CGTTTGGCTG CCCTGCCCAA AGGGCGGCCA GACCGACCCA GGGACCTGGA GCTCACTGAC 
CTGGCGGAAA GGAGTGTGAG GCTGACCTGG ATCCCGGGGG ATGATAACAA CAGTCCCATC 
ACAGACTACG TTGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGAGTCTG GCATGACCAC 
TCCAAGTTCC CAGGCAGTGT CAACTCAGCC GTCCTCCATC TGTCCCCGTA TGTCAACTAT 
CAGTTCCGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG 
CGGTACCGAA CAAGCGGGGC ACCCCCTGAA TCCAACCCCA GTGATGTGAA GGGCGAAGGG 
ACACGAAAAA ACAATATGGA GATCACATGG ACGCCTATGA ATGCTACCTC CGCCTTTGGC 
CCCAACCTGC GCTACATTGT CAAGTGGCGA CGGAGAGAAA CCCGAGAGAC TTGGAACAAT 
GTCACCGTGT GGGGCTCTCG CTATGTGGTG GGGCAGACCC CTGTCTACGT ACCCTATGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCGG AGCCTGAAAC CGTCATTGGG 
TACTCGGGGG AAGATTATCC CAGGGCTGCA CCCACTGAAG TTAAAATCCG AGTCCTGAAC 
AGCACAGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCTG ACACGGTCCA GGGCCAGCTC 
AGAGAGTATC GAGCTTACTA CTGGAGGGAA AGCAGTTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGAC AGCAGGCCAG CTTCCCTGGC GACCGCCCCC GGGGCGTGGT GGGTCGCCTG 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG TGATGGGCCT 
CGAAGTGAAA CCAAGGAATT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
AGAGTCCGAC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGATCACCC AGAGCACCCC 
AACGGGATCC TGATTGGATA CACGCTCAGA TACGTGCCCT TTAATGGAAC CAAACTGGGA 
AAGCAGATGG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCTCGGTGCA GAGAGCAGAC 
CCCGTGTCCC GTTACCGCTT CTCCCTCAGT GCCAGGACGC AGGTGGGCTC TGGAGAAGCA 
GCCACGGAGG AGTCCCCAAC ACCTCCAAAT GAAGCTTACA CCAACAACCA GACTGACATC 
GCCACCCAGG GCTGGTTCAT CGGGCTCATG TGTGCCATTG CCCTTCTGGT GCTGATCCTG 
CTTATCGTCT GCTTCATCAA GAGGAGTCGT GGCGGCAAGT ACCCAGTGCG AGAAAAGAAG 
GATGTCCCCT TGGGCCCTGA AGACCCCAAA GAAGAAGATG GTTCATTTGA CTACAGTGAC 
GAGGACAACA AGCCCCTGCA GGGCAGCCAG ACATCTCTGG ACGGCACCAT CAAGCAGCAG 
GAGAGTGACG ACAGCCTAGT GGACTACGGC GAGGGTGGCG AGGGCCAGTT CAACGAAGAT 
GGCTCCTTTA TTGGCCAGTA CACTGTCAGA AAGGACAAGG AGGAGACCGA GGGCAATGAG 
AGCTCAGAGG CCACATCTCC AGTCAATGCC ATCTATTCCC TGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454136.1
CDS	50..3937
Translation	MRRLAARAAAGDAGEVAAPGADIWDCAQGWSWSSWRLTDLCAIWEPGVFLPSAAAVNRSRASAGVAKSRPNEAEKLSTVWKAHEERRKASRARGLGSAEQGLALRMARQQAPPWVHVALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWLVNGDPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPETVIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVGRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPTPPNEAYTNNQTDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVRKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017598647.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurofascin (Nfasc), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598647.1

ORF Insert Sequence:

ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA 
TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA 
GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG 
AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG 
GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC 
CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT 
GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC 
CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG 
ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG 
TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG 
TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG 
GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT 
GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG 
GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA 
CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC 
AACAACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATATATAG TGCCCGAGGA 
GTTGCGGAAA GAACACCTAG CTTCATGTAT CCCCAGGGCA CGTCAAGCAG TCAGATGGTA 
CTGCGCGGCA TGGACCTGCT GCTGGAGTGC ATTGCCTCTG GGGTCCCAAC ACCAGATATT 
GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTCGA GAATTTTAAC 
AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCCCCATAC 
TGGCTGGATG AGCCCAAGAA CTTGATCCTG GCTCCTGGTG AAGATGGGAG GCTGGTGTGT 
CGAGCCAATG GGAACCCGAA GCCGACCGTC CAGTGGCTGG TGAATGGAGA CCCTTTGCAA 
TCGGCACCAC CCAACCCCAA CCGTGAGGTG GCCGGAGACA CTATCATCTT CCGGGACACT 
CAGATCAGCA GCAGGGCAGT GTACCAGTGT AACACATCCA ACGAACATGG CTACCTGCTG 
GCCAATGCCT TCGTCAGCGT ATTAGATGTA CCCCCTCGGA TGCTGTCTCC CCGGAACCAG 
CTCATCAGGG TGATCCTTTA CAACCGGACG CGACTGGACT GTCCGTTCTT TGGGTCTCCC 
ATTCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG TGGTAACTAC 
CACGTCTACG AAAATGGCAG CCTGGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC 
TACACCTGTG TGGCCACCAA CATCCTGGGA AAAGCTGAAA ATCAAGTCCG CCTGGAGGTC 
AAAGACCCCA CCAGGATCTA CAGGATGCCT GAAGACCAGG TGGCCAAGAG GGGCACCACA 
GTGCAGCTGG AGTGCCGTGT GAAGCATGAC CCCTCCTTGA AACTCACAGT CTCCTGGCTG 
AAGGATGACG AGCCACTCTA CATTGGAAAC AGGATGAAAA AGGAAGATGA CTCCCTGACC 
ATCTTCGGAG TGGCAGAGCG GGACCAAGGC AGTTACACGT GCATGGCCAG CACCGAGCTG 
GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCGACTAAC 
CGTTTGGCTG CCCTGCCCAA AGGGCGGCCA GACCGACCCA GGGACCTGGA GCTCACTGAC 
CTGGCGGAAA GGAGTGTGAG GCTGACCTGG ATCCCGGGGG ATGATAACAA CAGTCCCATC 
ACAGACTACG TTGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGAGTCTG GCATGACCAC 
TCCAAGTTCC CAGGCAGTGT CAACTCAGCC GTCCTCCATC TGTCCCCGTA TGTCAACTAT 
CAGTTCCGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG 
CGGTACCGAA CAAGCGGGGC ACCCCCTGAA TCCAACCCCA GTGATGTGAA GGGCGAAGGG 
ACACGAAAAA ACAATATGGA GATCACATGG ACGCCTATGA ATGCTACCTC CGCCTTTGGC 
CCCAACCTGC GCTACATTGT CAAGTGGCGA CGGAGAGAAA CCCGAGAGAC TTGGAACAAT 
GTCACCGTGT GGGGCTCTCG CTATGTGGTG GGGCAGACCC CTGTCTACGT ACCCTATGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCGG AGCCTGAAAC CGTCATTGGG 
TACTCGGGGG AAGATTATCC CAGGGCTGCA CCCACTGAAG TTAAAATCCG AGTCCTGAAC 
AGCACAGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCTG ACACGGTCCA GGGCCAGCTC 
AGAGAGTATC GAGCTTACTA CTGGAGGGAA AGCAGTTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGAC AGCAGGCCAG CTTCCCTGGC GACCGCCCCC GGGGCGTGGT GGGTCGCCTG 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG TGATGGGCCT 
CGAAGTGAAA CCAAGGAATT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
AGAGTCCGAC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGATCACCC AGAGCACCCC 
AACGGGATCC TGATTGGATA CACGCTCAGA TACGTGCCCT TTAATGGAAC CAAACTGGGA 
AAGCAGATGG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCTCGGTGCA GAGAGCAGAC 
CCCGTGTCCC GTTACCGCTT CTCCCTCAGT GCCAGGACGC AGGTGGGCTC TGGAGAAGCA 
GCCACGGAGG AGTCCCCAAC ACCTCCAAAT GAAGCTTACA CCAACAACCA GACTGACATC 
GCCACCCAGG GCTGGTTCAT CGGGCTCATG TGTGCCATTG CCCTTCTGGT GCTGATCCTG 
CTTATCGTCT GCTTCATCAA GAGGAGTCGT GGCGGCAAGT ACCCAGTGCG AGAAAAGAAG 
GATGTCCCCT TGGGCCCTGA AGACCCCAAA GAAGAAGATG GTTCATTTGA CTACAGTGAC 
GAGGACAACA AGCCCCTGCA GGGCAGCCAG ACATCTCTGG ACGGCACCAT CAAGCAGCAG 
GAGAGTGACG ACAGCCTAGT GGACTACGGC GAGGGTGGCG AGGGCCAGTT CAACGAAGAT 
GGCTCCTTTA TTGGCCAGTA CACTGTCAGA AAGGACAAGG AGGAGACCGA GGGCAATGAG 
AGCTCAGAGG CCACATCTCC AGTCAATGCC ATCTATTCCC TGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_017598648.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454137.1 Rattus norvegicus neurofascin (Nfasc), transcript variant X13, mRNA.

Price & Availability↑

CloneID	ORa57985
Clone ID Related Accession (Same CDS sequence)	XM_017598648.1
Accession Version	XM_017598648.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neurofascin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA 
TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA 
GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG 
AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG 
GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC 
CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCTGAC CCAACCCCCA 
ACGATCACCA AGCAGTCGGT GAAGGACCAC ATCGTGGACC CCCGAGATAA CATCCTGATT 
GAATGTGAAG CTAAAGGGAA CCCCGCCCCC AGTTTCCACT GGACTCGCAA CAGCAGGTTC 
TTCAACATTG CCAAGGACCC ACGGGTGTCC ATGAGGAGGA GGTCTGGGAC CTTGGTGATC 
GACTTCCGCA GTGGTGGGCG GCCTGAGGAG TACGAAGGGG AGTACCAGTG CTTTGCCCGG 
AACAAATTCG GCACAGCTCT TAGCAACCGC ATCCGCCTGC AGGTGTCCAA ATCTCCCCTG 
TGGCCCAAGG AAAACCTAGA CCCCGTCGTG GTTCAAGAGG GTGCCCCCTT AACCCTGCAG 
TGCAACCCCC CACCTGGCCT CCCATCCCCC GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AGGACAAGCG TGTCTCCCAG GGTCACAACG GGGACCTGTA CTTCTCCAAC 
GTCATGCTGC AGGACATGCA GACCGACTAC AGCTGCAATG CACGCTTCCA CTTCACCCAC 
ACCATTCAGC AGAAGAATCC CTTCACCCTC AAAGTCCTCA CCAACAACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATATAT AGTGCCCGAG GAGTTGCGGA AAGAACACCT 
AGCTTCATGT ATCCCCAGGG CACGTCAAGC AGTCAGATGG TACTGCGCGG CATGGACCTG 
CTGCTGGAGT GCATTGCCTC TGGGGTCCCA ACACCAGATA TTGCATGGTA CAAGAAAGGT 
GGGGACCTCC CATCTGACAA GGCCAAGTTC GAGAATTTTA ACAAGGCTCT GCGCATCACC 
AATGTCTCTG AAGAGGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGC 
ATCCGGCACA CGATCTCGGT GAGAGTAAAG GCTGCCCCAT ACTGGCTGGA TGAGCCCAAG 
AACTTGATCC TGGCTCCTGG TGAAGATGGG AGGCTGGTGT GTCGAGCCAA TGGGAACCCG 
AAGCCGACCG TCCAGTGGCT GGTGAATGGA GACCCTTTGC AATCGGCACC ACCCAACCCC 
AACCGTGAGG TGGCCGGAGA CACTATCATC TTCCGGGACA CTCAGATCAG CAGCAGGGCA 
GTGTACCAGT GTAACACATC CAACGAACAT GGCTACCTGC TGGCCAATGC CTTCGTCAGC 
GTATTAGATG TACCCCCTCG GATGCTGTCT CCCCGGAACC AGCTCATCAG GGTGATCCTT 
TACAACCGGA CGCGACTGGA CTGTCCGTTC TTTGGGTCTC CCATTCCAAC ACTCCGATGG 
TTTAAGAATG GGCAAGGAAG CAACCTGGAT GGTGGTAACT ACCACGTCTA CGAAAATGGC 
AGCCTGGAAA TCAAGATGAT TCGCAAAGAG GACCAAGGCA TCTACACCTG TGTGGCCACC 
AACATCCTGG GAAAAGCTGA AAATCAAGTC CGCCTGGAGG TCAAAGACCC CACCAGGATC 
TACAGGATGC CTGAAGACCA GGTGGCCAAG AGGGGCACCA CAGTGCAGCT GGAGTGCCGT 
GTGAAGCATG ACCCCTCCTT GAAACTCACA GTCTCCTGGC TGAAGGATGA CGAGCCACTC 
TACATTGGAA ACAGGATGAA AAAGGAAGAT GACTCCCTGA CCATCTTCGG AGTGGCAGAG 
CGGGACCAAG GCAGTTACAC GTGCATGGCC AGCACCGAGC TGGACCAGGA CCTGGCAAAG 
GCCTACCTCA CTGTTCTAGC TGATCAGGCC ACTCCGACTA ACCGTTTGGC TGCCCTGCCC 
AAAGGGCGGC CAGACCGACC CAGGGACCTG GAGCTCACTG ACCTGGCGGA AAGGAGTGTG 
AGGCTGACCT GGATCCCGGG GGATGATAAC AACAGTCCCA TCACAGACTA CGTTGTCCAG 
TTTGAAGAGG ACCAGTTCCA GCCAGGAGTC TGGCATGACC ACTCCAAGTT CCCAGGCAGT 
GTCAACTCAG CCGTCCTCCA TCTGTCCCCG TATGTCAACT ATCAGTTCCG AGTCATCGCT 
GTCAACGAGG TTGGGAGCAG CCACCCCAGC CTTCCATCCG AGCGGTACCG AACAAGCGGG 
GCACCCCCTG AATCCAACCC CAGTGATGTG AAGGGCGAAG GGACACGAAA AAACAATATG 
GAGATCACAT GGACGCCTAT GAATGCTACC TCCGCCTTTG GCCCCAACCT GCGCTACATT 
GTCAAGTGGC GACGGAGAGA AACCCGAGAG ACTTGGAACA ATGTCACCGT GTGGGGCTCT 
CGCTATGTGG TGGGGCAGAC CCCTGTCTAC GTACCCTATG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAAGGCCC GGAGCCTGAA ACCGTCATTG GGTACTCGGG GGAAGATTAT 
CCCAGGGCTG CACCCACTGA AGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC TGACACGGTC CAGGGCCAGC TCAGAGAGTA TCGAGCTTAC 
TACTGGAGGG AAAGCAGTTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAGGCC 
AGCTTCCCTG GCGACCGCCC CCGGGGCGTG GTGGGTCGCC TGTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGAGA GGTGATGGGC CTCGAAGTGA AACCAAGGAA 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCAGAGTCCG ACAGCCCAAC 
CTGGAGACCA TCAACCTGGA ATGGGATCAC CCAGAGCACC CCAACGGGAT CCTGATTGGA 
TACACGCTCA GATACGTGCC CTTTAATGGA ACCAAACTGG GAAAGCAGAT GGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCTCGGTG CAGAGAGCAG ACCCCGTGTC CCGTTACCGC 
TTCTCCCTCA GTGCCAGGAC GCAGGTGGGC TCTGGAGAAG CAGCCACGGA GGAGTCCCCA 
ACACCTCCAA ATGAAGCTAC TCCAACTGCA GCTTACACCA ACAACCAGAC TGACATCGCC 
ACCCAGGGCT GGTTCATCGG GCTCATGTGT GCCATTGCCC TTCTGGTGCT GATCCTGCTT 
ATCGTCTGCT TCATCAAGAG GAGTCGTGGC GGCAAGTACC CAGTGCGAGA AAAGAAGGAT 
GTCCCCTTGG GCCCTGAAGA CCCCAAAGAA GAAGATGGTT CATTTGACTA CAGTGACGAG 
GACAACAAGC CCCTGCAGGG CAGCCAGACA TCTCTGGACG GCACCATCAA GCAGCAGGAG 
AGTGACGACA GCCTAGTGGA CTACGGCGAG GGTGGCGAGG GCCAGTTCAA CGAAGATGGC 
TCCTTTATTG GCCAGTACAC TGTCAGAAAG GACAAGGAGG AGACCGAGGG CAATGAGAGC 
TCAGAGGCCA CATCTCCAGT CAATGCCATC TATTCCCTGG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454137.1
CDS	51..3935
Translation	MRRLAARAAAGDAGEVAAPGADIWDCAQGWSWSSWRLTDLCAIWEPGVFLPSAAAVNRSRASAGVAKSRPNEAEKLSTVWKAHEERRKASRARGLGSAEQGLALRMARQQAPPWVHVALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWLVNGDPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPETVIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVGRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPTPPNEATPTAAYTNNQTDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVRKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017598648.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurofascin (Nfasc), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598648.1

ORF Insert Sequence:

ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA 
TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA 
GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG 
AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG 
GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC 
CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCTGAC CCAACCCCCA 
ACGATCACCA AGCAGTCGGT GAAGGACCAC ATCGTGGACC CCCGAGATAA CATCCTGATT 
GAATGTGAAG CTAAAGGGAA CCCCGCCCCC AGTTTCCACT GGACTCGCAA CAGCAGGTTC 
TTCAACATTG CCAAGGACCC ACGGGTGTCC ATGAGGAGGA GGTCTGGGAC CTTGGTGATC 
GACTTCCGCA GTGGTGGGCG GCCTGAGGAG TACGAAGGGG AGTACCAGTG CTTTGCCCGG 
AACAAATTCG GCACAGCTCT TAGCAACCGC ATCCGCCTGC AGGTGTCCAA ATCTCCCCTG 
TGGCCCAAGG AAAACCTAGA CCCCGTCGTG GTTCAAGAGG GTGCCCCCTT AACCCTGCAG 
TGCAACCCCC CACCTGGCCT CCCATCCCCC GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AGGACAAGCG TGTCTCCCAG GGTCACAACG GGGACCTGTA CTTCTCCAAC 
GTCATGCTGC AGGACATGCA GACCGACTAC AGCTGCAATG CACGCTTCCA CTTCACCCAC 
ACCATTCAGC AGAAGAATCC CTTCACCCTC AAAGTCCTCA CCAACAACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATATAT AGTGCCCGAG GAGTTGCGGA AAGAACACCT 
AGCTTCATGT ATCCCCAGGG CACGTCAAGC AGTCAGATGG TACTGCGCGG CATGGACCTG 
CTGCTGGAGT GCATTGCCTC TGGGGTCCCA ACACCAGATA TTGCATGGTA CAAGAAAGGT 
GGGGACCTCC CATCTGACAA GGCCAAGTTC GAGAATTTTA ACAAGGCTCT GCGCATCACC 
AATGTCTCTG AAGAGGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGC 
ATCCGGCACA CGATCTCGGT GAGAGTAAAG GCTGCCCCAT ACTGGCTGGA TGAGCCCAAG 
AACTTGATCC TGGCTCCTGG TGAAGATGGG AGGCTGGTGT GTCGAGCCAA TGGGAACCCG 
AAGCCGACCG TCCAGTGGCT GGTGAATGGA GACCCTTTGC AATCGGCACC ACCCAACCCC 
AACCGTGAGG TGGCCGGAGA CACTATCATC TTCCGGGACA CTCAGATCAG CAGCAGGGCA 
GTGTACCAGT GTAACACATC CAACGAACAT GGCTACCTGC TGGCCAATGC CTTCGTCAGC 
GTATTAGATG TACCCCCTCG GATGCTGTCT CCCCGGAACC AGCTCATCAG GGTGATCCTT 
TACAACCGGA CGCGACTGGA CTGTCCGTTC TTTGGGTCTC CCATTCCAAC ACTCCGATGG 
TTTAAGAATG GGCAAGGAAG CAACCTGGAT GGTGGTAACT ACCACGTCTA CGAAAATGGC 
AGCCTGGAAA TCAAGATGAT TCGCAAAGAG GACCAAGGCA TCTACACCTG TGTGGCCACC 
AACATCCTGG GAAAAGCTGA AAATCAAGTC CGCCTGGAGG TCAAAGACCC CACCAGGATC 
TACAGGATGC CTGAAGACCA GGTGGCCAAG AGGGGCACCA CAGTGCAGCT GGAGTGCCGT 
GTGAAGCATG ACCCCTCCTT GAAACTCACA GTCTCCTGGC TGAAGGATGA CGAGCCACTC 
TACATTGGAA ACAGGATGAA AAAGGAAGAT GACTCCCTGA CCATCTTCGG AGTGGCAGAG 
CGGGACCAAG GCAGTTACAC GTGCATGGCC AGCACCGAGC TGGACCAGGA CCTGGCAAAG 
GCCTACCTCA CTGTTCTAGC TGATCAGGCC ACTCCGACTA ACCGTTTGGC TGCCCTGCCC 
AAAGGGCGGC CAGACCGACC CAGGGACCTG GAGCTCACTG ACCTGGCGGA AAGGAGTGTG 
AGGCTGACCT GGATCCCGGG GGATGATAAC AACAGTCCCA TCACAGACTA CGTTGTCCAG 
TTTGAAGAGG ACCAGTTCCA GCCAGGAGTC TGGCATGACC ACTCCAAGTT CCCAGGCAGT 
GTCAACTCAG CCGTCCTCCA TCTGTCCCCG TATGTCAACT ATCAGTTCCG AGTCATCGCT 
GTCAACGAGG TTGGGAGCAG CCACCCCAGC CTTCCATCCG AGCGGTACCG AACAAGCGGG 
GCACCCCCTG AATCCAACCC CAGTGATGTG AAGGGCGAAG GGACACGAAA AAACAATATG 
GAGATCACAT GGACGCCTAT GAATGCTACC TCCGCCTTTG GCCCCAACCT GCGCTACATT 
GTCAAGTGGC GACGGAGAGA AACCCGAGAG ACTTGGAACA ATGTCACCGT GTGGGGCTCT 
CGCTATGTGG TGGGGCAGAC CCCTGTCTAC GTACCCTATG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAAGGCCC GGAGCCTGAA ACCGTCATTG GGTACTCGGG GGAAGATTAT 
CCCAGGGCTG CACCCACTGA AGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGCCTT 
CAGTGGAACC GCGTCTACTC TGACACGGTC CAGGGCCAGC TCAGAGAGTA TCGAGCTTAC 
TACTGGAGGG AAAGCAGTTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAGGCC 
AGCTTCCCTG GCGACCGCCC CCGGGGCGTG GTGGGTCGCC TGTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGAGA GGTGATGGGC CTCGAAGTGA AACCAAGGAA 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCAGAGTCCG ACAGCCCAAC 
CTGGAGACCA TCAACCTGGA ATGGGATCAC CCAGAGCACC CCAACGGGAT CCTGATTGGA 
TACACGCTCA GATACGTGCC CTTTAATGGA ACCAAACTGG GAAAGCAGAT GGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCTCGGTG CAGAGAGCAG ACCCCGTGTC CCGTTACCGC 
TTCTCCCTCA GTGCCAGGAC GCAGGTGGGC TCTGGAGAAG CAGCCACGGA GGAGTCCCCA 
ACACCTCCAA ATGAAGCTAC TCCAACTGCA GCTTACACCA ACAACCAGAC TGACATCGCC 
ACCCAGGGCT GGTTCATCGG GCTCATGTGT GCCATTGCCC TTCTGGTGCT GATCCTGCTT 
ATCGTCTGCT TCATCAAGAG GAGTCGTGGC GGCAAGTACC CAGTGCGAGA AAAGAAGGAT 
GTCCCCTTGG GCCCTGAAGA CCCCAAAGAA GAAGATGGTT CATTTGACTA CAGTGACGAG 
GACAACAAGC CCCTGCAGGG CAGCCAGACA TCTCTGGACG GCACCATCAA GCAGCAGGAG 
AGTGACGACA GCCTAGTGGA CTACGGCGAG GGTGGCGAGG GCCAGTTCAA CGAAGATGGC 
TCCTTTATTG GCCAGTACAC TGTCAGAAAG GACAAGGAGG AGACCGAGGG CAATGAGAGC 
TCAGAGGCCA CATCTCCAGT CAATGCCATC TATTCCCTGG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_017598649.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454138.1 Rattus norvegicus neurofascin (Nfasc), transcript variant X15, mRNA.

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CloneID	ORa57986
Clone ID Related Accession (Same CDS sequence)	XM_017598649.1
Accession Version	XM_017598649.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neurofascin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA 
TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA 
GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG 
AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG 
GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC 
CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCTGAC CCAACCCCCA 
ACGATCACCA AGCAGTCGGT GAAGGACCAC ATCGTGGACC CCCGAGATAA CATCCTGATT 
GAATGTGAAG CTAAAGGGAA CCCCGCCCCC AGTTTCCACT GGACTCGCAA CAGCAGGTTC 
TTCAACATTG CCAAGGACCC ACGGGTGTCC ATGAGGAGGA GGTCTGGGAC CTTGGTGATC 
GACTTCCGCA GTGGTGGGCG GCCTGAGGAG TACGAAGGGG AGTACCAGTG CTTTGCCCGG 
AACAAATTCG GCACAGCTCT TAGCAACCGC ATCCGCCTGC AGGTGTCCAA ATCTCCCCTG 
TGGCCCAAGG AAAACCTAGA CCCCGTCGTG GTTCAAGAGG GTGCCCCCTT AACCCTGCAG 
TGCAACCCCC CACCTGGCCT CCCATCCCCC GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AGGACAAGCG TGTCTCCCAG GGTCACAACG GGGACCTGTA CTTCTCCAAC 
GTCATGCTGC AGGACATGCA GACCGACTAC AGCTGCAATG CACGCTTCCA CTTCACCCAC 
ACCATTCAGC AGAAGAATCC CTTCACCCTC AAAGTCCTCA CCAACAACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATATAT AGTGCCCGAG GAGTTGCGGA AAGAACACCT 
AGCTTCATGT ATCCCCAGGG CACGTCAAGC AGTCAGATGG TACTGCGCGG CATGGACCTG 
CTGCTGGAGT GCATTGCCTC TGGGGTCCCA ACACCAGATA TTGCATGGTA CAAGAAAGGT 
GGGGACCTCC CATCTGACAA GGCCAAGTTC GAGAATTTTA ACAAGGCTCT GCGCATCACC 
AATGTCTCTG AAGAGGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGC 
ATCCGGCACA CGATCTCGGT GAGAGTAAAG GCTGCCCCAT ACTGGCTGGA TGAGCCCAAG 
AACTTGATCC TGGCTCCTGG TGAAGATGGG AGGCTGGTGT GTCGAGCCAA TGGGAACCCG 
AAGCCGACCG TCCAGTGGCT GGTGAATGGA GACCCTTTGC AATCGGCACC ACCCAACCCC 
AACCGTGAGG TGGCCGGAGA CACTATCATC TTCCGGGACA CTCAGATCAG CAGCAGGGCA 
GTGTACCAGT GTAACACATC CAACGAACAT GGCTACCTGC TGGCCAATGC CTTCGTCAGC 
GTATTAGATG TACCCCCTCG GATGCTGTCT CCCCGGAACC AGCTCATCAG GGTGATCCTT 
TACAACCGGA CGCGACTGGA CTGTCCGTTC TTTGGGTCTC CCATTCCAAC ACTCCGATGG 
TTTAAGAATG GGCAAGGAAG CAACCTGGAT GGTGGTAACT ACCACGTCTA CGAAAATGGC 
AGCCTGGAAA TCAAGATGAT TCGCAAAGAG GACCAAGGCA TCTACACCTG TGTGGCCACC 
AACATCCTGG GAAAAGCTGA AAATCAAGTC CGCCTGGAGG TCAAAGACCC CACCAGGATC 
TACAGGATGC CTGAAGACCA GGTGGCCAAG AGGGGCACCA CAGTGCAGCT GGAGTGCCGT 
GTGAAGCATG ACCCCTCCTT GAAACTCACA GTCTCCTGGC TGAAGGATGA CGAGCCACTC 
TACATTGGAA ACAGGATGAA AAAGGAAGAT GACTCCCTGA CCATCTTCGG AGTGGCAGAG 
CGGGACCAAG GCAGTTACAC GTGCATGGCC AGCACCGAGC TGGACCAGGA CCTGGCAAAG 
GCCTACCTCA CTGTTCTAGG GCGGCCAGAC CGACCCAGGG ACCTGGAGCT CACTGACCTG 
GCGGAAAGGA GTGTGAGGCT GACCTGGATC CCGGGGGATG ATAACAACAG TCCCATCACA 
GACTACGTTG TCCAGTTTGA AGAGGACCAG TTCCAGCCAG GAGTCTGGCA TGACCACTCC 
AAGTTCCCAG GCAGTGTCAA CTCAGCCGTC CTCCATCTGT CCCCGTATGT CAACTATCAG 
TTCCGAGTCA TCGCTGTCAA CGAGGTTGGG AGCAGCCACC CCAGCCTTCC ATCCGAGCGG 
TACCGAACAA GCGGGGCACC CCCTGAATCC AACCCCAGTG ATGTGAAGGG CGAAGGGACA 
CGAAAAAACA ATATGGAGAT CACATGGACG CCTATGAATG CTACCTCCGC CTTTGGCCCC 
AACCTGCGCT ACATTGTCAA GTGGCGACGG AGAGAAACCC GAGAGACTTG GAACAATGTC 
ACCGTGTGGG GCTCTCGCTA TGTGGTGGGG CAGACCCCTG TCTACGTACC CTATGAGATC 
CGAGTCCAGG CTGAAAATGA CTTTGGGAAA GGCCCGGAGC CTGAAACCGT CATTGGGTAC 
TCGGGGGAAG ATTATCCCAG GGCTGCACCC ACTGAAGTTA AAATCCGAGT CCTGAACAGC 
ACAGCCATCA GCCTTCAGTG GAACCGCGTC TACTCTGACA CGGTCCAGGG CCAGCTCAGA 
GAGTATCGAG CTTACTACTG GAGGGAAAGC AGTTTGCTGA AGAACCTGTG GGTGTCTCAG 
AAGAGACAGC AGGCCAGCTT CCCTGGCGAC CGCCCCCGGG GCGTGGTGGG TCGCCTGTTC 
CCCTACAGTA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGAGAGGTGA TGGGCCTCGA 
AGTGAAACCA AGGAATTCAC CACCCCGGAA GGAGTACCCA GTGCCCCCAG GCGTTTCAGA 
GTCCGACAGC CCAACCTGGA GACCATCAAC CTGGAATGGG ATCACCCAGA GCACCCCAAC 
GGGATCCTGA TTGGATACAC GCTCAGATAC GTGCCCTTTA ATGGAACCAA ACTGGGAAAG 
CAGATGGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCT CGGTGCAGAG AGCAGACCCC 
GTGTCCCGTT ACCGCTTCTC CCTCAGTGCC AGGACGCAGG TGGGCTCTGG AGAAGCAGCC 
ACGGAGGAGT CCCCAACACC TCCAAATGAA GCTTACACCA ACAACCAGAC TGACATCGCC 
ACCCAGGGCT GGTTCATCGG GCTCATGTGT GCCATTGCCC TTCTGGTGCT GATCCTGCTT 
ATCGTCTGCT TCATCAAGAG GAGTCGTGGC GGCAAGTACC CAGTGCGAGA AAAGAAGGAT 
GTCCCCTTGG GCCCTGAAGA CCCCAAAGAA GAAGATGGTT CATTTGACTA CAGTGACGAG 
GACAACAAGC CCCTGCAGGG CAGCCAGACA TCTCTGGACG GCACCATCAA GCAGCAGGAG 
AGTGACGACA GCCTAGTGGA CTACGGCGAG GGTGGCGAGG GCCAGTTCAA CGAAGATGGC 
TCCTTTATTG GCCAGTACAC TGTCAGAAAG GACAAGGAGG AGACCGAGGG CAATGAGAGC 
TCAGAGGCCA CATCTCCAGT CAATGCCATC TATTCCCTGG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454138.1
CDS	50..3874
Translation	MRRLAARAAAGDAGEVAAPGADIWDCAQGWSWSSWRLTDLCAIWEPGVFLPSAAAVNRSRASAGVAKSRPNEAEKLSTVWKAHEERRKASRARGLGSAEQGLALRMARQQAPPWVHVALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWLVNGDPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPETVIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVGRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPTPPNEAYTNNQTDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVRKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017598649.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurofascin (Nfasc), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598649.1

ORF Insert Sequence:

ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA 
TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA 
GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG 
AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG 
GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC 
CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCTGAC CCAACCCCCA 
ACGATCACCA AGCAGTCGGT GAAGGACCAC ATCGTGGACC CCCGAGATAA CATCCTGATT 
GAATGTGAAG CTAAAGGGAA CCCCGCCCCC AGTTTCCACT GGACTCGCAA CAGCAGGTTC 
TTCAACATTG CCAAGGACCC ACGGGTGTCC ATGAGGAGGA GGTCTGGGAC CTTGGTGATC 
GACTTCCGCA GTGGTGGGCG GCCTGAGGAG TACGAAGGGG AGTACCAGTG CTTTGCCCGG 
AACAAATTCG GCACAGCTCT TAGCAACCGC ATCCGCCTGC AGGTGTCCAA ATCTCCCCTG 
TGGCCCAAGG AAAACCTAGA CCCCGTCGTG GTTCAAGAGG GTGCCCCCTT AACCCTGCAG 
TGCAACCCCC CACCTGGCCT CCCATCCCCC GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AGGACAAGCG TGTCTCCCAG GGTCACAACG GGGACCTGTA CTTCTCCAAC 
GTCATGCTGC AGGACATGCA GACCGACTAC AGCTGCAATG CACGCTTCCA CTTCACCCAC 
ACCATTCAGC AGAAGAATCC CTTCACCCTC AAAGTCCTCA CCAACAACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATATAT AGTGCCCGAG GAGTTGCGGA AAGAACACCT 
AGCTTCATGT ATCCCCAGGG CACGTCAAGC AGTCAGATGG TACTGCGCGG CATGGACCTG 
CTGCTGGAGT GCATTGCCTC TGGGGTCCCA ACACCAGATA TTGCATGGTA CAAGAAAGGT 
GGGGACCTCC CATCTGACAA GGCCAAGTTC GAGAATTTTA ACAAGGCTCT GCGCATCACC 
AATGTCTCTG AAGAGGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGC 
ATCCGGCACA CGATCTCGGT GAGAGTAAAG GCTGCCCCAT ACTGGCTGGA TGAGCCCAAG 
AACTTGATCC TGGCTCCTGG TGAAGATGGG AGGCTGGTGT GTCGAGCCAA TGGGAACCCG 
AAGCCGACCG TCCAGTGGCT GGTGAATGGA GACCCTTTGC AATCGGCACC ACCCAACCCC 
AACCGTGAGG TGGCCGGAGA CACTATCATC TTCCGGGACA CTCAGATCAG CAGCAGGGCA 
GTGTACCAGT GTAACACATC CAACGAACAT GGCTACCTGC TGGCCAATGC CTTCGTCAGC 
GTATTAGATG TACCCCCTCG GATGCTGTCT CCCCGGAACC AGCTCATCAG GGTGATCCTT 
TACAACCGGA CGCGACTGGA CTGTCCGTTC TTTGGGTCTC CCATTCCAAC ACTCCGATGG 
TTTAAGAATG GGCAAGGAAG CAACCTGGAT GGTGGTAACT ACCACGTCTA CGAAAATGGC 
AGCCTGGAAA TCAAGATGAT TCGCAAAGAG GACCAAGGCA TCTACACCTG TGTGGCCACC 
AACATCCTGG GAAAAGCTGA AAATCAAGTC CGCCTGGAGG TCAAAGACCC CACCAGGATC 
TACAGGATGC CTGAAGACCA GGTGGCCAAG AGGGGCACCA CAGTGCAGCT GGAGTGCCGT 
GTGAAGCATG ACCCCTCCTT GAAACTCACA GTCTCCTGGC TGAAGGATGA CGAGCCACTC 
TACATTGGAA ACAGGATGAA AAAGGAAGAT GACTCCCTGA CCATCTTCGG AGTGGCAGAG 
CGGGACCAAG GCAGTTACAC GTGCATGGCC AGCACCGAGC TGGACCAGGA CCTGGCAAAG 
GCCTACCTCA CTGTTCTAGG GCGGCCAGAC CGACCCAGGG ACCTGGAGCT CACTGACCTG 
GCGGAAAGGA GTGTGAGGCT GACCTGGATC CCGGGGGATG ATAACAACAG TCCCATCACA 
GACTACGTTG TCCAGTTTGA AGAGGACCAG TTCCAGCCAG GAGTCTGGCA TGACCACTCC 
AAGTTCCCAG GCAGTGTCAA CTCAGCCGTC CTCCATCTGT CCCCGTATGT CAACTATCAG 
TTCCGAGTCA TCGCTGTCAA CGAGGTTGGG AGCAGCCACC CCAGCCTTCC ATCCGAGCGG 
TACCGAACAA GCGGGGCACC CCCTGAATCC AACCCCAGTG ATGTGAAGGG CGAAGGGACA 
CGAAAAAACA ATATGGAGAT CACATGGACG CCTATGAATG CTACCTCCGC CTTTGGCCCC 
AACCTGCGCT ACATTGTCAA GTGGCGACGG AGAGAAACCC GAGAGACTTG GAACAATGTC 
ACCGTGTGGG GCTCTCGCTA TGTGGTGGGG CAGACCCCTG TCTACGTACC CTATGAGATC 
CGAGTCCAGG CTGAAAATGA CTTTGGGAAA GGCCCGGAGC CTGAAACCGT CATTGGGTAC 
TCGGGGGAAG ATTATCCCAG GGCTGCACCC ACTGAAGTTA AAATCCGAGT CCTGAACAGC 
ACAGCCATCA GCCTTCAGTG GAACCGCGTC TACTCTGACA CGGTCCAGGG CCAGCTCAGA 
GAGTATCGAG CTTACTACTG GAGGGAAAGC AGTTTGCTGA AGAACCTGTG GGTGTCTCAG 
AAGAGACAGC AGGCCAGCTT CCCTGGCGAC CGCCCCCGGG GCGTGGTGGG TCGCCTGTTC 
CCCTACAGTA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGAGAGGTGA TGGGCCTCGA 
AGTGAAACCA AGGAATTCAC CACCCCGGAA GGAGTACCCA GTGCCCCCAG GCGTTTCAGA 
GTCCGACAGC CCAACCTGGA GACCATCAAC CTGGAATGGG ATCACCCAGA GCACCCCAAC 
GGGATCCTGA TTGGATACAC GCTCAGATAC GTGCCCTTTA ATGGAACCAA ACTGGGAAAG 
CAGATGGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCT CGGTGCAGAG AGCAGACCCC 
GTGTCCCGTT ACCGCTTCTC CCTCAGTGCC AGGACGCAGG TGGGCTCTGG AGAAGCAGCC 
ACGGAGGAGT CCCCAACACC TCCAAATGAA GCTTACACCA ACAACCAGAC TGACATCGCC 
ACCCAGGGCT GGTTCATCGG GCTCATGTGT GCCATTGCCC TTCTGGTGCT GATCCTGCTT 
ATCGTCTGCT TCATCAAGAG GAGTCGTGGC GGCAAGTACC CAGTGCGAGA AAAGAAGGAT 
GTCCCCTTGG GCCCTGAAGA CCCCAAAGAA GAAGATGGTT CATTTGACTA CAGTGACGAG 
GACAACAAGC CCCTGCAGGG CAGCCAGACA TCTCTGGACG GCACCATCAA GCAGCAGGAG 
AGTGACGACA GCCTAGTGGA CTACGGCGAG GGTGGCGAGG GCCAGTTCAA CGAAGATGGC 
TCCTTTATTG GCCAGTACAC TGTCAGAAAG GACAAGGAGG AGACCGAGGG CAATGAGAGC 
TCAGAGGCCA CATCTCCAGT CAATGCCATC TATTCCCTGG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_017598650.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454139.1 Rattus norvegicus neurofascin (Nfasc), transcript variant X16, mRNA.

Price & Availability↑

CloneID	ORa57987
Clone ID Related Accession (Same CDS sequence)	XM_017598650.1
Accession Version	XM_017598650.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neurofascin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA 
TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA 
GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG 
AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG 
GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC 
CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCTGAC CCAACCCCCA 
ACGATCACCA AGCAGTCGGT GAAGGACCAC ATCGTGGACC CCCGAGATAA CATCCTGATT 
GAATGTGAAG CTAAAGGGAA CCCCGCCCCC AGTTTCCACT GGACTCGCAA CAGCAGGTTC 
TTCAACATTG CCAAGGACCC ACGGGTGTCC ATGAGGAGGA GGTCTGGGAC CTTGGTGATC 
GACTTCCGCA GTGGTGGGCG GCCTGAGGAG TACGAAGGGG AGTACCAGTG CTTTGCCCGG 
AACAAATTCG GCACAGCTCT TAGCAACCGC ATCCGCCTGC AGGTGTCCAA ATCTCCCCTG 
TGGCCCAAGG AAAACCTAGA CCCCGTCGTG GTTCAAGAGG GTGCCCCCTT AACCCTGCAG 
TGCAACCCCC CACCTGGCCT CCCATCCCCC GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AGGACAAGCG TGTCTCCCAG GGTCACAACG GGGACCTGTA CTTCTCCAAC 
GTCATGCTGC AGGACATGCA GACCGACTAC AGCTGCAATG CACGCTTCCA CTTCACCCAC 
ACCATTCAGC AGAAGAATCC CTTCACCCTC AAAGTCCTCA CCACCCGAGG AGTTGCGGAA 
AGAACACCTA GCTTCATGTA TCCCCAGGGC ACGTCAAGCA GTCAGATGGT ACTGCGCGGC 
ATGGACCTGC TGCTGGAGTG CATTGCCTCT GGGGTCCCAA CACCAGATAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTCG AGAATTTTAA CAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCCCCATA CTGGCTGGAT 
GAGCCCAAGA ACTTGATCCT GGCTCCTGGT GAAGATGGGA GGCTGGTGTG TCGAGCCAAT 
GGGAACCCGA AGCCGACCGT CCAGTGGCTG GTGAATGGAG ACCCTTTGCA ATCGGCACCA 
CCCAACCCCA ACCGTGAGGT GGCCGGAGAC ACTATCATCT TCCGGGACAC TCAGATCAGC 
AGCAGGGCAG TGTACCAGTG TAACACATCC AACGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TATTAGATGT ACCCCCTCGG ATGCTGTCTC CCCGGAACCA GCTCATCAGG 
GTGATCCTTT ACAACCGGAC GCGACTGGAC TGTCCGTTCT TTGGGTCTCC CATTCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GTGGTAACTA CCACGTCTAC 
GAAAATGGCA GCCTGGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG AAAAGCTGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC TGAAGACCAG GTGGCCAAGA GGGGCACCAC AGTGCAGCTG 
GAGTGCCGTG TGAAGCATGA CCCCTCCTTG AAACTCACAG TCTCCTGGCT GAAGGATGAC 
GAGCCACTCT ACATTGGAAA CAGGATGAAA AAGGAAGATG ACTCCCTGAC CATCTTCGGA 
GTGGCAGAGC GGGACCAAGG CAGTTACACG TGCATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGGG CGGCCAGACC GACCCAGGGA CCTGGAGCTC 
ACTGACCTGG CGGAAAGGAG TGTGAGGCTG ACCTGGATCC CGGGGGATGA TAACAACAGT 
CCCATCACAG ACTACGTTGT CCAGTTTGAA GAGGACCAGT TCCAGCCAGG AGTCTGGCAT 
GACCACTCCA AGTTCCCAGG CAGTGTCAAC TCAGCCGTCC TCCATCTGTC CCCGTATGTC 
AACTATCAGT TCCGAGTCAT CGCTGTCAAC GAGGTTGGGA GCAGCCACCC CAGCCTTCCA 
TCCGAGCGGT ACCGAACAAG CGGGGCACCC CCTGAATCCA ACCCCAGTGA TGTGAAGGGC 
GAAGGGACAC GAAAAAACAA TATGGAGATC ACATGGACGC CTATGAATGC TACCTCCGCC 
TTTGGCCCCA ACCTGCGCTA CATTGTCAAG TGGCGACGGA GAGAAACCCG AGAGACTTGG 
AACAATGTCA CCGTGTGGGG CTCTCGCTAT GTGGTGGGGC AGACCCCTGT CTACGTACCC 
TATGAGATCC GAGTCCAGGC TGAAAATGAC TTTGGGAAAG GCCCGGAGCC TGAAACCGTC 
ATTGGGTACT CGGGGGAAGA TTATCCCAGG GCTGCACCCA CTGAAGTTAA AATCCGAGTC 
CTGAACAGCA CAGCCATCAG CCTTCAGTGG AACCGCGTCT ACTCTGACAC GGTCCAGGGC 
CAGCTCAGAG AGTATCGAGC TTACTACTGG AGGGAAAGCA GTTTGCTGAA GAACCTGTGG 
GTGTCTCAGA AGAGACAGCA GGCCAGCTTC CCTGGCGACC GCCCCCGGGG CGTGGTGGGT 
CGCCTGTTCC CCTACAGTAA CTACAAGCTG GAGATGGTTG TGGTCAATGG GAGAGGTGAT 
GGGCCTCGAA GTGAAACCAA GGAATTCACC ACCCCGGAAG GAGTACCCAG TGCCCCCAGG 
CGTTTCAGAG TCCGACAGCC CAACCTGGAG ACCATCAACC TGGAATGGGA TCACCCAGAG 
CACCCCAACG GGATCCTGAT TGGATACACG CTCAGATACG TGCCCTTTAA TGGAACCAAA 
CTGGGAAAGC AGATGGTGGA AAACTTCTCT CCCAATCAGA CCAAGTTCTC GGTGCAGAGA 
GCAGACCCCG TGTCCCGTTA CCGCTTCTCC CTCAGTGCCA GGACGCAGGT GGGCTCTGGA 
GAAGCAGCCA CGGAGGAGTC CCCAACACCT CCAAATGAAG CTACTCCAAC TGCAGCTTAC 
ACCAACAACC AGACTGACAT CGCCACCCAG GGCTGGTTCA TCGGGCTCAT GTGTGCCATT 
GCCCTTCTGG TGCTGATCCT GCTTATCGTC TGCTTCATCA AGAGGAGTCG TGGCGGCAAG 
TACCCAGTGC GAGAAAAGAA GGATGTCCCC TTGGGCCCTG AAGACCCCAA AGAAGAAGAT 
GGTTCATTTG ACTACAGTGA CGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACATCTCTG 
GACGGCACCA TCAAGCAGCA GGAGAGTGAC GACAGCCTAG TGGACTACGG CGAGGGTGGC 
GAGGGCCAGT TCAACGAAGA TGGCTCCTTT ATTGGCCAGT ACACTGTCAG AAAGGACAAG 
GAGGAGACCG AGGGCAATGA GAGCTCAGAG GCCACATCTC CAGTCAATGC CATCTATTCC 
CTGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454139.1
CDS	49..3837
Translation	MRRLAARAAAGDAGEVAAPGADIWDCAQGWSWSSWRLTDLCAIWEPGVFLPSAAAVNRSRASAGVAKSRPNEAEKLSTVWKAHEERRKASRARGLGSAEQGLALRMARQQAPPWVHVALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWLVNGDPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPETVIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVGRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPTPPNEATPTAAYTNNQTDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVRKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017598650.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurofascin (Nfasc), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598650.1

ORF Insert Sequence:

ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA 
TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA 
GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG 
AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG 
GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC 
CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCTGAC CCAACCCCCA 
ACGATCACCA AGCAGTCGGT GAAGGACCAC ATCGTGGACC CCCGAGATAA CATCCTGATT 
GAATGTGAAG CTAAAGGGAA CCCCGCCCCC AGTTTCCACT GGACTCGCAA CAGCAGGTTC 
TTCAACATTG CCAAGGACCC ACGGGTGTCC ATGAGGAGGA GGTCTGGGAC CTTGGTGATC 
GACTTCCGCA GTGGTGGGCG GCCTGAGGAG TACGAAGGGG AGTACCAGTG CTTTGCCCGG 
AACAAATTCG GCACAGCTCT TAGCAACCGC ATCCGCCTGC AGGTGTCCAA ATCTCCCCTG 
TGGCCCAAGG AAAACCTAGA CCCCGTCGTG GTTCAAGAGG GTGCCCCCTT AACCCTGCAG 
TGCAACCCCC CACCTGGCCT CCCATCCCCC GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AGGACAAGCG TGTCTCCCAG GGTCACAACG GGGACCTGTA CTTCTCCAAC 
GTCATGCTGC AGGACATGCA GACCGACTAC AGCTGCAATG CACGCTTCCA CTTCACCCAC 
ACCATTCAGC AGAAGAATCC CTTCACCCTC AAAGTCCTCA CCACCCGAGG AGTTGCGGAA 
AGAACACCTA GCTTCATGTA TCCCCAGGGC ACGTCAAGCA GTCAGATGGT ACTGCGCGGC 
ATGGACCTGC TGCTGGAGTG CATTGCCTCT GGGGTCCCAA CACCAGATAT TGCATGGTAC 
AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTCG AGAATTTTAA CAAGGCTCTG 
CGCATCACCA ATGTCTCTGA AGAGGACTCT GGGGAGTATT TCTGCCTGGC CTCCAACAAG 
ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCCCCATA CTGGCTGGAT 
GAGCCCAAGA ACTTGATCCT GGCTCCTGGT GAAGATGGGA GGCTGGTGTG TCGAGCCAAT 
GGGAACCCGA AGCCGACCGT CCAGTGGCTG GTGAATGGAG ACCCTTTGCA ATCGGCACCA 
CCCAACCCCA ACCGTGAGGT GGCCGGAGAC ACTATCATCT TCCGGGACAC TCAGATCAGC 
AGCAGGGCAG TGTACCAGTG TAACACATCC AACGAACATG GCTACCTGCT GGCCAATGCC 
TTCGTCAGCG TATTAGATGT ACCCCCTCGG ATGCTGTCTC CCCGGAACCA GCTCATCAGG 
GTGATCCTTT ACAACCGGAC GCGACTGGAC TGTCCGTTCT TTGGGTCTCC CATTCCAACA 
CTCCGATGGT TTAAGAATGG GCAAGGAAGC AACCTGGATG GTGGTAACTA CCACGTCTAC 
GAAAATGGCA GCCTGGAAAT CAAGATGATT CGCAAAGAGG ACCAAGGCAT CTACACCTGT 
GTGGCCACCA ACATCCTGGG AAAAGCTGAA AATCAAGTCC GCCTGGAGGT CAAAGACCCC 
ACCAGGATCT ACAGGATGCC TGAAGACCAG GTGGCCAAGA GGGGCACCAC AGTGCAGCTG 
GAGTGCCGTG TGAAGCATGA CCCCTCCTTG AAACTCACAG TCTCCTGGCT GAAGGATGAC 
GAGCCACTCT ACATTGGAAA CAGGATGAAA AAGGAAGATG ACTCCCTGAC CATCTTCGGA 
GTGGCAGAGC GGGACCAAGG CAGTTACACG TGCATGGCCA GCACCGAGCT GGACCAGGAC 
CTGGCAAAGG CCTACCTCAC TGTTCTAGGG CGGCCAGACC GACCCAGGGA CCTGGAGCTC 
ACTGACCTGG CGGAAAGGAG TGTGAGGCTG ACCTGGATCC CGGGGGATGA TAACAACAGT 
CCCATCACAG ACTACGTTGT CCAGTTTGAA GAGGACCAGT TCCAGCCAGG AGTCTGGCAT 
GACCACTCCA AGTTCCCAGG CAGTGTCAAC TCAGCCGTCC TCCATCTGTC CCCGTATGTC 
AACTATCAGT TCCGAGTCAT CGCTGTCAAC GAGGTTGGGA GCAGCCACCC CAGCCTTCCA 
TCCGAGCGGT ACCGAACAAG CGGGGCACCC CCTGAATCCA ACCCCAGTGA TGTGAAGGGC 
GAAGGGACAC GAAAAAACAA TATGGAGATC ACATGGACGC CTATGAATGC TACCTCCGCC 
TTTGGCCCCA ACCTGCGCTA CATTGTCAAG TGGCGACGGA GAGAAACCCG AGAGACTTGG 
AACAATGTCA CCGTGTGGGG CTCTCGCTAT GTGGTGGGGC AGACCCCTGT CTACGTACCC 
TATGAGATCC GAGTCCAGGC TGAAAATGAC TTTGGGAAAG GCCCGGAGCC TGAAACCGTC 
ATTGGGTACT CGGGGGAAGA TTATCCCAGG GCTGCACCCA CTGAAGTTAA AATCCGAGTC 
CTGAACAGCA CAGCCATCAG CCTTCAGTGG AACCGCGTCT ACTCTGACAC GGTCCAGGGC 
CAGCTCAGAG AGTATCGAGC TTACTACTGG AGGGAAAGCA GTTTGCTGAA GAACCTGTGG 
GTGTCTCAGA AGAGACAGCA GGCCAGCTTC CCTGGCGACC GCCCCCGGGG CGTGGTGGGT 
CGCCTGTTCC CCTACAGTAA CTACAAGCTG GAGATGGTTG TGGTCAATGG GAGAGGTGAT 
GGGCCTCGAA GTGAAACCAA GGAATTCACC ACCCCGGAAG GAGTACCCAG TGCCCCCAGG 
CGTTTCAGAG TCCGACAGCC CAACCTGGAG ACCATCAACC TGGAATGGGA TCACCCAGAG 
CACCCCAACG GGATCCTGAT TGGATACACG CTCAGATACG TGCCCTTTAA TGGAACCAAA 
CTGGGAAAGC AGATGGTGGA AAACTTCTCT CCCAATCAGA CCAAGTTCTC GGTGCAGAGA 
GCAGACCCCG TGTCCCGTTA CCGCTTCTCC CTCAGTGCCA GGACGCAGGT GGGCTCTGGA 
GAAGCAGCCA CGGAGGAGTC CCCAACACCT CCAAATGAAG CTACTCCAAC TGCAGCTTAC 
ACCAACAACC AGACTGACAT CGCCACCCAG GGCTGGTTCA TCGGGCTCAT GTGTGCCATT 
GCCCTTCTGG TGCTGATCCT GCTTATCGTC TGCTTCATCA AGAGGAGTCG TGGCGGCAAG 
TACCCAGTGC GAGAAAAGAA GGATGTCCCC TTGGGCCCTG AAGACCCCAA AGAAGAAGAT 
GGTTCATTTG ACTACAGTGA CGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACATCTCTG 
GACGGCACCA TCAAGCAGCA GGAGAGTGAC GACAGCCTAG TGGACTACGG CGAGGGTGGC 
GAGGGCCAGT TCAACGAAGA TGGCTCCTTT ATTGGCCAGT ACACTGTCAG AAAGGACAAG 
GAGGAGACCG AGGGCAATGA GAGCTCAGAG GCCACATCTC CAGTCAATGC CATCTATTCC 
CTGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( NM_001160314.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001153786.1 Rattus norvegicus neurofascin (Nfasc), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa12769
Clone ID Related Accession (Same CDS sequence)	NM_001160314.1
Accession Version	NM_001160314.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	neurofascin isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AABR07072325.1, AC134289.4, AY061639.1, U81036.1, AC105703.6 and BF410020.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). This isoform lacks the third fibronectin type 3 (FNIII) repeat and includes the mucin-like domain. Isoform 1 is also known as NF186, as described by Davis et al. (PMID: 8947556). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACGTAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATTGAGAT TCCGATGGAT CCAAGCATTC AGAATGAGCT GACCCAACCC 
CCAACGATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ACCCCCGAGA TAACATCCTG 
ATTGAATGTG AAGCTAAAGG GAACCCCGCC CCCAGTTTCC ACTGGACTCG CAACAGCAGG 
TTCTTCAACA TTGCCAAGGA CCCACGGGTG TCCATGAGGA GGAGGTCTGG GACCTTGGTG 
ATCGACTTCC GCAGTGGTGG GCGGCCTGAG GAGTACGAAG GGGAGTACCA GTGCTTTGCC 
CGGAACAAAT TCGGCACAGC TCTTAGCAAC CGCATCCGCC TGCAGGTGTC CAAATCTCCC 
CTGTGGCCCA AGGAAAACCT AGACCCCGTC GTGGTTCAAG AGGGTGCCCC CTTAACCCTG 
CAGTGCAACC CCCCACCTGG CCTCCCATCC CCCGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAGGACAA GCGTGTCTCC CAGGGTCACA ACGGGGACCT GTACTTCTCC 
AACGTCATGC TGCAGGACAT GCAGACCGAC TACAGCTGCA ATGCACGCTT CCACTTCACC 
CACACCATTC AGCAGAAGAA TCCCTTCACC CTCAAAGTCC TCACCACCCG AGGAGTTGCG 
GAAAGAACAC CTAGCTTCAT GTATCCCCAG GGCACGTCAA GCAGTCAGAT GGTACTGCGC 
GGCATGGACC TGCTGCTGGA GTGCATTGCC TCTGGGGTCC CAACACCAGA TATTGCATGG 
TACAAGAAAG GTGGGGACCT CCCATCTGAC AAGGCCAAGT TCGAGAATTT TAACAAGGCT 
CTGCGCATCA CCAATGTCTC TGAAGAGGAC TCTGGGGAGT ATTTCTGCCT GGCCTCCAAC 
AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCCCC ATACTGGCTG 
GATGAGCCCA AGAACTTGAT CCTGGCTCCT GGTGAAGATG GGAGGCTGGT GTGTCGAGCC 
AATGGGAACC CGAAGCCGAC CGTCCAGTGG CTGGTGAATG GAGACCCTTT GCAATCGGCA 
CCACCCAACC CCAACCGTGA GGTGGCCGGA GACACTATCA TCTTCCGGGA CACTCAGATC 
AGCAGCAGGG CAGTGTACCA GTGTAACACA TCCAACGAAC ATGGCTACCT GCTGGCCAAT 
GCCTTCGTCA GCGTATTAGA TGTACCCCCT CGGATGCTGT CTCCCCGGAA CCAGCTCATC 
AGGGTGATCC TTTACAACCG GACGCGACTG GACTGTCCGT TCTTTGGGTC TCCCATTCCA 
ACACTCCGAT GGTTTAAGAA TGGGCAAGGA AGCAACCTGG ATGGTGGTAA CTACCACGTC 
TACGAAAATG GCAGCCTGGA AATCAAGATG ATTCGCAAAG AGGACCAAGG CATCTACACC 
TGTGTGGCCA CCAACATCCT GGGAAAAGCT GAAAATCAAG TCCGCCTGGA GGTCAAAGAC 
CCCACCAGGA TCTACAGGAT GCCTGAAGAC CAGGTGGCCA AGAGGGGCAC CACAGTGCAG 
CTGGAGTGCC GTGTGAAGCA TGACCCCTCC TTGAAACTCA CAGTCTCCTG GCTGAAGGAT 
GACGAGCCAC TCTACATTGG AAACAGGATG AAAAAGGAAG ATGACTCCCT GACCATCTTC 
GGAGTGGCAG AGCGGGACCA AGGCAGTTAC ACGTGCATGG CCAGCACCGA GCTGGACCAG 
GACCTGGCAA AGGCCTACCT CACTGTTCTA GCTGATCAGG CCACTCCGAC TAACCGTTTG 
GCTGCCCTGC CCAAAGGGCG GCCAGACCGA CCCAGGGACC TGGAGCTCAC TGACCTGGCG 
GAAAGGAGTG TGAGGCTGAC CTGGATCCCG GGGGATGATA ACAACAGTCC CATCACAGAC 
TACGTTGTCC AGTTTGAAGA GGACCAGTTC CAGCCAGGAG TCTGGCATGA CCACTCCAAG 
TTCCCAGGCA GTGTCAACTC AGCCGTCCTC CATCTGTCCC CGTATGTCAA CTATCAGTTC 
CGAGTCATCG CTGTCAACGA GGTTGGGAGC AGCCACCCCA GCCTTCCATC CGAGCGGTAC 
CGAACAAGCG GGGCACCCCC TGAATCCAAC CCCAGTGATG TGAAGGGCGA AGGGACACGA 
AAAAACAATA TGGAGATCAC ATGGACGCCT ATGAATGCTA CCTCCGCCTT TGGCCCCAAC 
CTGCGCTACA TTGTCAAGTG GCGACGGAGA GAAACCCGAG AGACTTGGAA CAATGTCACC 
GTGTGGGGCT CTCGCTATGT GGTGGGGCAG ACCCCTGTCT ACGTACCCTA TGAGATCCGA 
GTCCAGGCTG AAAATGACTT TGGGAAAGGC CCGGAGCCTG AAACCGTCAT TGGGTACTCG 
GGGGAAGATT TACCCAGTGC CCCCAGGCGT TTCAGAGTCC GACAGCCCAA CCTGGAGACC 
ATCAACCTGG AATGGGATCA CCCAGAGCAC CCCAACGGGA TCCTGATTGG ATACACGCTC 
AGATACGTGC CCTTTAATGG AACCAAACTG GGAAAGCAGA TGGTGGAAAA CTTCTCTCCC 
AATCAGACCA AGTTCTCGGT GCAGAGAGCA GACCCCGTGT CCCGTTACCG CTTCTCCCTC 
AGTGCCAGGA CGCAGGTGGG CTCTGGAGAA GCAGCCACGG AGGAGTCCCC AACACCTCCA 
AATGAAGCTA CTCCAACTGC AGCTCCTCCC ACATTGCCCC CGACTACCGT GGGTACCACA 
GGCCTTGTGA GCAGTACTGA TGCTACTGCC CTTGCTGCCA CCAGTGAAGC CACAACAGTT 
CCCATCATCC CAACGGTGGT ACCTACCACC GTCGCCACCA CCATTGCCAC AACTACTACA 
ACCACTGCCG CCGCCACCAC CACTACCACC ACGGAGAGTC CTCCCACTAC CACCACTGGG 
ACTAAGATTC ACGAAACCGC CCCCGACGAG CAGTCCATTT GGAACGTCAC GGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CACCTGGAAG CACAATTTCA GGCCTGGAAC TGACTTTGTG 
GTTGAGTACA TCGACAGCAA CCATACGAAA AAAACTGTCC CTGTTAAGGC CCAAGCCCAG 
CCCATACAGC TGACAGACCT CTTTCCCGGG ATGACGTACA CGTTGCGGGT GTATTCCCGG 
GACAACGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAC 
AACCAGACTG ACATCGCCAC CCAGGGCTGG TTCATCGGGC TCATGTGTGC CATTGCCCTT 
CTGGTGCTGA TCCTGCTGAT CGTCTGCTTC ATCAAGAGGA GTCGTGGCGG CAAGTACCCA 
GTGCGAGAAA AGAAGGATGT CCCCTTGGGC CCTGAAGACC CCAAAGAAGA AGATGGTTCA 
TTTGACTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACATC TCTGGACGGC 
ACCATCAAGC AGCAGGAGAG TGACGACAGC CTAGTGGACT ACGGCGAGGG TGGCGAGGGC 
CAGTTCAACG AAGATGGCTC CTTTATTGGC CAGTACACTG TCAGAAAGGA CAAGGAGGAG 
ACCGAGGGCA ATGAGAGCTC AGAGGCCACA TCTCCAGTCA ATGCCATCTA TTCCCTGGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153786.1
CDS	309..4031
Translation	MARQQAPPWVHVALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWLVNGDPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPETVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPTPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAAATTTTTTESPPTTTTGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQTDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVRKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	NM_001160314.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurofascin (Nfasc), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160314.1

ORF Insert Sequence:

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACGTAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATTGAGAT TCCGATGGAT CCAAGCATTC AGAATGAGCT GACCCAACCC 
CCAACGATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ACCCCCGAGA TAACATCCTG 
ATTGAATGTG AAGCTAAAGG GAACCCCGCC CCCAGTTTCC ACTGGACTCG CAACAGCAGG 
TTCTTCAACA TTGCCAAGGA CCCACGGGTG TCCATGAGGA GGAGGTCTGG GACCTTGGTG 
ATCGACTTCC GCAGTGGTGG GCGGCCTGAG GAGTACGAAG GGGAGTACCA GTGCTTTGCC 
CGGAACAAAT TCGGCACAGC TCTTAGCAAC CGCATCCGCC TGCAGGTGTC CAAATCTCCC 
CTGTGGCCCA AGGAAAACCT AGACCCCGTC GTGGTTCAAG AGGGTGCCCC CTTAACCCTG 
CAGTGCAACC CCCCACCTGG CCTCCCATCC CCCGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAGGACAA GCGTGTCTCC CAGGGTCACA ACGGGGACCT GTACTTCTCC 
AACGTCATGC TGCAGGACAT GCAGACCGAC TACAGCTGCA ATGCACGCTT CCACTTCACC 
CACACCATTC AGCAGAAGAA TCCCTTCACC CTCAAAGTCC TCACCACCCG AGGAGTTGCG 
GAAAGAACAC CTAGCTTCAT GTATCCCCAG GGCACGTCAA GCAGTCAGAT GGTACTGCGC 
GGCATGGACC TGCTGCTGGA GTGCATTGCC TCTGGGGTCC CAACACCAGA TATTGCATGG 
TACAAGAAAG GTGGGGACCT CCCATCTGAC AAGGCCAAGT TCGAGAATTT TAACAAGGCT 
CTGCGCATCA CCAATGTCTC TGAAGAGGAC TCTGGGGAGT ATTTCTGCCT GGCCTCCAAC 
AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCCCC ATACTGGCTG 
GATGAGCCCA AGAACTTGAT CCTGGCTCCT GGTGAAGATG GGAGGCTGGT GTGTCGAGCC 
AATGGGAACC CGAAGCCGAC CGTCCAGTGG CTGGTGAATG GAGACCCTTT GCAATCGGCA 
CCACCCAACC CCAACCGTGA GGTGGCCGGA GACACTATCA TCTTCCGGGA CACTCAGATC 
AGCAGCAGGG CAGTGTACCA GTGTAACACA TCCAACGAAC ATGGCTACCT GCTGGCCAAT 
GCCTTCGTCA GCGTATTAGA TGTACCCCCT CGGATGCTGT CTCCCCGGAA CCAGCTCATC 
AGGGTGATCC TTTACAACCG GACGCGACTG GACTGTCCGT TCTTTGGGTC TCCCATTCCA 
ACACTCCGAT GGTTTAAGAA TGGGCAAGGA AGCAACCTGG ATGGTGGTAA CTACCACGTC 
TACGAAAATG GCAGCCTGGA AATCAAGATG ATTCGCAAAG AGGACCAAGG CATCTACACC 
TGTGTGGCCA CCAACATCCT GGGAAAAGCT GAAAATCAAG TCCGCCTGGA GGTCAAAGAC 
CCCACCAGGA TCTACAGGAT GCCTGAAGAC CAGGTGGCCA AGAGGGGCAC CACAGTGCAG 
CTGGAGTGCC GTGTGAAGCA TGACCCCTCC TTGAAACTCA CAGTCTCCTG GCTGAAGGAT 
GACGAGCCAC TCTACATTGG AAACAGGATG AAAAAGGAAG ATGACTCCCT GACCATCTTC 
GGAGTGGCAG AGCGGGACCA AGGCAGTTAC ACGTGCATGG CCAGCACCGA GCTGGACCAG 
GACCTGGCAA AGGCCTACCT CACTGTTCTA GCTGATCAGG CCACTCCGAC TAACCGTTTG 
GCTGCCCTGC CCAAAGGGCG GCCAGACCGA CCCAGGGACC TGGAGCTCAC TGACCTGGCG 
GAAAGGAGTG TGAGGCTGAC CTGGATCCCG GGGGATGATA ACAACAGTCC CATCACAGAC 
TACGTTGTCC AGTTTGAAGA GGACCAGTTC CAGCCAGGAG TCTGGCATGA CCACTCCAAG 
TTCCCAGGCA GTGTCAACTC AGCCGTCCTC CATCTGTCCC CGTATGTCAA CTATCAGTTC 
CGAGTCATCG CTGTCAACGA GGTTGGGAGC AGCCACCCCA GCCTTCCATC CGAGCGGTAC 
CGAACAAGCG GGGCACCCCC TGAATCCAAC CCCAGTGATG TGAAGGGCGA AGGGACACGA 
AAAAACAATA TGGAGATCAC ATGGACGCCT ATGAATGCTA CCTCCGCCTT TGGCCCCAAC 
CTGCGCTACA TTGTCAAGTG GCGACGGAGA GAAACCCGAG AGACTTGGAA CAATGTCACC 
GTGTGGGGCT CTCGCTATGT GGTGGGGCAG ACCCCTGTCT ACGTACCCTA TGAGATCCGA 
GTCCAGGCTG AAAATGACTT TGGGAAAGGC CCGGAGCCTG AAACCGTCAT TGGGTACTCG 
GGGGAAGATT TACCCAGTGC CCCCAGGCGT TTCAGAGTCC GACAGCCCAA CCTGGAGACC 
ATCAACCTGG AATGGGATCA CCCAGAGCAC CCCAACGGGA TCCTGATTGG ATACACGCTC 
AGATACGTGC CCTTTAATGG AACCAAACTG GGAAAGCAGA TGGTGGAAAA CTTCTCTCCC 
AATCAGACCA AGTTCTCGGT GCAGAGAGCA GACCCCGTGT CCCGTTACCG CTTCTCCCTC 
AGTGCCAGGA CGCAGGTGGG CTCTGGAGAA GCAGCCACGG AGGAGTCCCC AACACCTCCA 
AATGAAGCTA CTCCAACTGC AGCTCCTCCC ACATTGCCCC CGACTACCGT GGGTACCACA 
GGCCTTGTGA GCAGTACTGA TGCTACTGCC CTTGCTGCCA CCAGTGAAGC CACAACAGTT 
CCCATCATCC CAACGGTGGT ACCTACCACC GTCGCCACCA CCATTGCCAC AACTACTACA 
ACCACTGCCG CCGCCACCAC CACTACCACC ACGGAGAGTC CTCCCACTAC CACCACTGGG 
ACTAAGATTC ACGAAACCGC CCCCGACGAG CAGTCCATTT GGAACGTCAC GGTGCTCCCC 
AACAGTAAAT GGGCCAACAT CACCTGGAAG CACAATTTCA GGCCTGGAAC TGACTTTGTG 
GTTGAGTACA TCGACAGCAA CCATACGAAA AAAACTGTCC CTGTTAAGGC CCAAGCCCAG 
CCCATACAGC TGACAGACCT CTTTCCCGGG ATGACGTACA CGTTGCGGGT GTATTCCCGG 
GACAACGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAC 
AACCAGACTG ACATCGCCAC CCAGGGCTGG TTCATCGGGC TCATGTGTGC CATTGCCCTT 
CTGGTGCTGA TCCTGCTGAT CGTCTGCTTC ATCAAGAGGA GTCGTGGCGG CAAGTACCCA 
GTGCGAGAAA AGAAGGATGT CCCCTTGGGC CCTGAAGACC CCAAAGAAGA AGATGGTTCA 
TTTGACTACA GTGACGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACATC TCTGGACGGC 
ACCATCAAGC AGCAGGAGAG TGACGACAGC CTAGTGGACT ACGGCGAGGG TGGCGAGGGC 
CAGTTCAACG AAGATGGCTC CTTTATTGGC CAGTACACTG TCAGAAAGGA CAAGGAGGAG 
ACCGAGGGCA ATGAGAGCTC AGAGGCCACA TCTCCAGTCA ATGCCATCTA TTCCCTGGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( NM_001160315.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001153787.1 Rattus norvegicus neurofascin (Nfasc), transcript variant 4, mRNA.

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CloneID	ORa12772
Clone ID Related Accession (Same CDS sequence)	NM_001160315.1
Accession Version	NM_001160315.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Rattus norvegicus(Norway rat)
Product	neurofascin isoform 4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AABR07072325.1, AC134289.4, AC105703.6 and BF410020.1. Transcript Variant: This variant (4) represents use of an alternate promoter and 5' UTR, and has multiple differences in the coding region but maintains the reading frame, compared to variant 1. The resulting isoform (4) includes the third fibronectin type 3 (FNIII) repeat, lacks the mucin-like domain, and lacks two segments in the N-terminal and central regions, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACGTAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATTGAGAT TCCGATGGAT CTGACCCAAC CCCCAACGAT CACCAAGCAG 
TCGGTGAAGG ACCACATCGT GGACCCCCGA GATAACATCC TGATTGAATG TGAAGCTAAA 
GGGAACCCCG CCCCCAGTTT CCACTGGACT CGCAACAGCA GGTTCTTCAA CATTGCCAAG 
GACCCACGGG TGTCCATGAG GAGGAGGTCT GGGACCTTGG TGATCGACTT CCGCAGTGGT 
GGGCGGCCTG AGGAGTACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTCGGCACA 
GCTCTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC 
CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTAACCC TGCAGTGCAA CCCCCCACCT 
GGCCTCCCAT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC 
AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC 
ATGCAGACCG ACTACAGCTG CAATGCACGC TTCCACTTCA CCCACACCAT TCAGCAGAAG 
AATCCCTTCA CCCTCAAAGT CCTCACCACC CGAGGAGTTG CGGAAAGAAC ACCTAGCTTC 
ATGTATCCCC AGGGCACGTC AAGCAGTCAG ATGGTACTGC GCGGCATGGA CCTGCTGCTG 
GAGTGCATTG CCTCTGGGGT CCCAACACCA GATATTGCAT GGTACAAGAA AGGTGGGGAC 
CTCCCATCTG ACAAGGCCAA GTTCGAGAAT TTTAACAAGG CTCTGCGCAT CACCAATGTC 
TCTGAAGAGG ACTCTGGGGA GTATTTCTGC CTGGCCTCCA ACAAGATGGG CAGCATCCGG 
CACACGATCT CGGTGAGAGT AAAGGCTGCC CCATACTGGC TGGATGAGCC CAAGAACTTG 
ATCCTGGCTC CTGGTGAAGA TGGGAGGCTG GTGTGTCGAG CCAATGGGAA CCCGAAGCCG 
ACCGTCCAGT GGCTGGTGAA TGGAGACCCT TTGCAATCGG CACCACCCAA CCCCAACCGT 
GAGGTGGCCG GAGACACTAT CATCTTCCGG GACACTCAGA TCAGCAGCAG GGCAGTGTAC 
CAGTGTAACA CATCCAACGA ACATGGCTAC CTGCTGGCCA ATGCCTTCGT CAGCGTATTA 
GATGTACCCC CTCGGATGCT GTCTCCCCGG AACCAGCTCA TCAGGGTGAT CCTTTACAAC 
CGGACGCGAC TGGACTGTCC GTTCTTTGGG TCTCCCATTC CAACACTCCG ATGGTTTAAG 
AATGGGCAAG GAAGCAACCT GGATGGTGGT AACTACCACG TCTACGAAAA TGGCAGCCTG 
GAAATCAAGA TGATTCGCAA AGAGGACCAA GGCATCTACA CCTGTGTGGC CACCAACATC 
CTGGGAAAAG CTGAAAATCA AGTCCGCCTG GAGGTCAAAG ACCCCACCAG GATCTACAGG 
ATGCCTGAAG ACCAGGTGGC CAAGAGGGGC ACCACAGTGC AGCTGGAGTG CCGTGTGAAG 
CATGACCCCT CCTTGAAACT CACAGTCTCC TGGCTGAAGG ATGACGAGCC ACTCTACATT 
GGAAACAGGA TGAAAAAGGA AGATGACTCC CTGACCATCT TCGGAGTGGC AGAGCGGGAC 
CAAGGCAGTT ACACGTGCAT GGCCAGCACC GAGCTGGACC AGGACCTGGC AAAGGCCTAC 
CTCACTGTTC TAGGGCGGCC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCGGAA 
AGGAGTGTGA GGCTGACCTG GATCCCGGGG GATGATAACA ACAGTCCCAT CACAGACTAC 
GTTGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGAGTCT GGCATGACCA CTCCAAGTTC 
CCAGGCAGTG TCAACTCAGC CGTCCTCCAT CTGTCCCCGT ATGTCAACTA TCAGTTCCGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACAAGCGGGG CACCCCCTGA ATCCAACCCC AGTGATGTGA AGGGCGAAGG GACACGAAAA 
AACAATATGG AGATCACATG GACGCCTATG AATGCTACCT CCGCCTTTGG CCCCAACCTG 
CGCTACATTG TCAAGTGGCG ACGGAGAGAA ACCCGAGAGA CTTGGAACAA TGTCACCGTG 
TGGGGCTCTC GCTATGTGGT GGGGCAGACC CCTGTCTACG TACCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCG GAGCCTGAAA CCGTCATTGG GTACTCGGGG 
GAAGATTATC CCAGGGCTGC ACCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC 
ATCAGCCTTC AGTGGAACCG CGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT 
CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA 
CAGCAGGCCA GCTTCCCTGG CGACCGCCCC CGGGGCGTGG TGGGTCGCCT GTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGAGAG GTGATGGGCC TCGAAGTGAA 
ACCAAGGAAT TCACCACCCC GGAAGGAGTA CCCAGTGCCC CCAGGCGTTT CAGAGTCCGA 
CAGCCCAACC TGGAGACCAT CAACCTGGAA TGGGATCACC CAGAGCACCC CAACGGGATC 
CTGATTGGAT ACACGCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCGGTGC AGAGAGCAGA CCCCGTGTCC 
CGTTACCGCT TCTCCCTCAG TGCCAGGACG CAGGTGGGCT CTGGAGAAGC AGCCACGGAG 
GAGTCCCCAA CACCTCCAAA TGAAGCTACT CCAACTGCAG CTTACACCAA CAACCAGACT 
GACATCGCCA CCCAGGGCTG GTTCATCGGG CTCATGTGTG CCATTGCCCT TCTGGTGCTG 
ATCCTGCTTA TCGTCTGCTT CATCAAGAGG AGTCGTGGCG GCAAGTACCC AGTGCGAGAA 
AAGAAGGATG TCCCCTTGGG CCCTGAAGAC CCCAAAGAAG AAGATGGTTC ATTTGACTAC 
AGTGACGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACAT CTCTGGACGG CACCATCAAG 
CAGCAGGAGA GTGACGACAG CCTAGTGGAC TACGGCGAGG GTGGCGAGGG CCAGTTCAAC 
GAAGATGGCT CCTTTATTGG CCAGTACACT GTCAGAAAGG ACAAGGAGGA GACCGAGGGC 
AATGAGAGCT CAGAGGCCAC ATCTCCAGTC AATGCCATCT ATTCCCTGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153787.1
CDS	518..3991
Translation	MARQQAPPWVHVALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWLVNGDPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPETVIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVGRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPTPPNEATPTAAYTNNQTDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVRKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	NM_001160315.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurofascin (Nfasc), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160315.1

ORF Insert Sequence:

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACGTAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATTGAGAT TCCGATGGAT CTGACCCAAC CCCCAACGAT CACCAAGCAG 
TCGGTGAAGG ACCACATCGT GGACCCCCGA GATAACATCC TGATTGAATG TGAAGCTAAA 
GGGAACCCCG CCCCCAGTTT CCACTGGACT CGCAACAGCA GGTTCTTCAA CATTGCCAAG 
GACCCACGGG TGTCCATGAG GAGGAGGTCT GGGACCTTGG TGATCGACTT CCGCAGTGGT 
GGGCGGCCTG AGGAGTACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTCGGCACA 
GCTCTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC 
CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTAACCC TGCAGTGCAA CCCCCCACCT 
GGCCTCCCAT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC 
AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC 
ATGCAGACCG ACTACAGCTG CAATGCACGC TTCCACTTCA CCCACACCAT TCAGCAGAAG 
AATCCCTTCA CCCTCAAAGT CCTCACCACC CGAGGAGTTG CGGAAAGAAC ACCTAGCTTC 
ATGTATCCCC AGGGCACGTC AAGCAGTCAG ATGGTACTGC GCGGCATGGA CCTGCTGCTG 
GAGTGCATTG CCTCTGGGGT CCCAACACCA GATATTGCAT GGTACAAGAA AGGTGGGGAC 
CTCCCATCTG ACAAGGCCAA GTTCGAGAAT TTTAACAAGG CTCTGCGCAT CACCAATGTC 
TCTGAAGAGG ACTCTGGGGA GTATTTCTGC CTGGCCTCCA ACAAGATGGG CAGCATCCGG 
CACACGATCT CGGTGAGAGT AAAGGCTGCC CCATACTGGC TGGATGAGCC CAAGAACTTG 
ATCCTGGCTC CTGGTGAAGA TGGGAGGCTG GTGTGTCGAG CCAATGGGAA CCCGAAGCCG 
ACCGTCCAGT GGCTGGTGAA TGGAGACCCT TTGCAATCGG CACCACCCAA CCCCAACCGT 
GAGGTGGCCG GAGACACTAT CATCTTCCGG GACACTCAGA TCAGCAGCAG GGCAGTGTAC 
CAGTGTAACA CATCCAACGA ACATGGCTAC CTGCTGGCCA ATGCCTTCGT CAGCGTATTA 
GATGTACCCC CTCGGATGCT GTCTCCCCGG AACCAGCTCA TCAGGGTGAT CCTTTACAAC 
CGGACGCGAC TGGACTGTCC GTTCTTTGGG TCTCCCATTC CAACACTCCG ATGGTTTAAG 
AATGGGCAAG GAAGCAACCT GGATGGTGGT AACTACCACG TCTACGAAAA TGGCAGCCTG 
GAAATCAAGA TGATTCGCAA AGAGGACCAA GGCATCTACA CCTGTGTGGC CACCAACATC 
CTGGGAAAAG CTGAAAATCA AGTCCGCCTG GAGGTCAAAG ACCCCACCAG GATCTACAGG 
ATGCCTGAAG ACCAGGTGGC CAAGAGGGGC ACCACAGTGC AGCTGGAGTG CCGTGTGAAG 
CATGACCCCT CCTTGAAACT CACAGTCTCC TGGCTGAAGG ATGACGAGCC ACTCTACATT 
GGAAACAGGA TGAAAAAGGA AGATGACTCC CTGACCATCT TCGGAGTGGC AGAGCGGGAC 
CAAGGCAGTT ACACGTGCAT GGCCAGCACC GAGCTGGACC AGGACCTGGC AAAGGCCTAC 
CTCACTGTTC TAGGGCGGCC AGACCGACCC AGGGACCTGG AGCTCACTGA CCTGGCGGAA 
AGGAGTGTGA GGCTGACCTG GATCCCGGGG GATGATAACA ACAGTCCCAT CACAGACTAC 
GTTGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGAGTCT GGCATGACCA CTCCAAGTTC 
CCAGGCAGTG TCAACTCAGC CGTCCTCCAT CTGTCCCCGT ATGTCAACTA TCAGTTCCGA 
GTCATCGCTG TCAACGAGGT TGGGAGCAGC CACCCCAGCC TTCCATCCGA GCGGTACCGA 
ACAAGCGGGG CACCCCCTGA ATCCAACCCC AGTGATGTGA AGGGCGAAGG GACACGAAAA 
AACAATATGG AGATCACATG GACGCCTATG AATGCTACCT CCGCCTTTGG CCCCAACCTG 
CGCTACATTG TCAAGTGGCG ACGGAGAGAA ACCCGAGAGA CTTGGAACAA TGTCACCGTG 
TGGGGCTCTC GCTATGTGGT GGGGCAGACC CCTGTCTACG TACCCTATGA GATCCGAGTC 
CAGGCTGAAA ATGACTTTGG GAAAGGCCCG GAGCCTGAAA CCGTCATTGG GTACTCGGGG 
GAAGATTATC CCAGGGCTGC ACCCACTGAA GTTAAAATCC GAGTCCTGAA CAGCACAGCC 
ATCAGCCTTC AGTGGAACCG CGTCTACTCT GACACGGTCC AGGGCCAGCT CAGAGAGTAT 
CGAGCTTACT ACTGGAGGGA AAGCAGTTTG CTGAAGAACC TGTGGGTGTC TCAGAAGAGA 
CAGCAGGCCA GCTTCCCTGG CGACCGCCCC CGGGGCGTGG TGGGTCGCCT GTTCCCCTAC 
AGTAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGAGAG GTGATGGGCC TCGAAGTGAA 
ACCAAGGAAT TCACCACCCC GGAAGGAGTA CCCAGTGCCC CCAGGCGTTT CAGAGTCCGA 
CAGCCCAACC TGGAGACCAT CAACCTGGAA TGGGATCACC CAGAGCACCC CAACGGGATC 
CTGATTGGAT ACACGCTCAG ATACGTGCCC TTTAATGGAA CCAAACTGGG AAAGCAGATG 
GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCTCGGTGC AGAGAGCAGA CCCCGTGTCC 
CGTTACCGCT TCTCCCTCAG TGCCAGGACG CAGGTGGGCT CTGGAGAAGC AGCCACGGAG 
GAGTCCCCAA CACCTCCAAA TGAAGCTACT CCAACTGCAG CTTACACCAA CAACCAGACT 
GACATCGCCA CCCAGGGCTG GTTCATCGGG CTCATGTGTG CCATTGCCCT TCTGGTGCTG 
ATCCTGCTTA TCGTCTGCTT CATCAAGAGG AGTCGTGGCG GCAAGTACCC AGTGCGAGAA 
AAGAAGGATG TCCCCTTGGG CCCTGAAGAC CCCAAAGAAG AAGATGGTTC ATTTGACTAC 
AGTGACGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACAT CTCTGGACGG CACCATCAAG 
CAGCAGGAGA GTGACGACAG CCTAGTGGAC TACGGCGAGG GTGGCGAGGG CCAGTTCAAC 
GAAGATGGCT CCTTTATTGG CCAGTACACT GTCAGAAAGG ACAAGGAGGA GACCGAGGGC 
AATGAGAGCT CAGAGGCCAC ATCTCCAGTC AATGCCATCT ATTCCCTGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( NM_053909.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446361.1 Rattus norvegicus neurofascin (Nfasc), transcript variant 3, mRNA.

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CloneID	ORa12778
Clone ID Related Accession (Same CDS sequence)	NM_053909.2
Accession Version	NM_053909.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Rattus norvegicus(Norway rat)
Product	neurofascin isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AABR07072325.1, AC134289.4, AY061639.1, AC105703.6 and BF410020.1. On May 21, 2009 this sequence version replaced NM_053909.1. Transcript Variant: This variant (3) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. The resulting isoform (3) includes the third fibronectin type 3 (FNIII) repeat, lacks the mucin-like domain, and has several differences in the N-terminal and central regions, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AY061639.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACGTAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATTGAGAT TCCGATGGAT CTGACCCAAC CCCCAACGAT CACCAAGCAG 
TCGGTGAAGG ACCACATCGT GGACCCCCGA GATAACATCC TGATTGAATG TGAAGCTAAA 
GGGAACCCCG CCCCCAGTTT CCACTGGACT CGCAACAGCA GGTTCTTCAA CATTGCCAAG 
GACCCACGGG TGTCCATGAG GAGGAGGTCT GGGACCTTGG TGATCGACTT CCGCAGTGGT 
GGGCGGCCTG AGGAGTACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTCGGCACA 
GCTCTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC 
CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTAACCC TGCAGTGCAA CCCCCCACCT 
GGCCTCCCAT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC 
AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC 
ATGCAGACCG ACTACAGCTG CAATGCACGC TTCCACTTCA CCCACACCAT TCAGCAGAAG 
AATCCCTTCA CCCTCAAAGT CCTCACCAAC AACCCCTATA ATGACTCGTC TTTAAGAAAC 
CACCCTGACA TATATAGTGC CCGAGGAGTT GCGGAAAGAA CACCTAGCTT CATGTATCCC 
CAGGGCACGT CAAGCAGTCA GATGGTACTG CGCGGCATGG ACCTGCTGCT GGAGTGCATT 
GCCTCTGGGG TCCCAACACC AGATATTGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
GACAAGGCCA AGTTCGAGAA TTTTAACAAG GCTCTGCGCA TCACCAATGT CTCTGAAGAG 
GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC CCCATACTGG CTGGATGAGC CCAAGAACTT GATCCTGGCT 
CCTGGTGAAG ATGGGAGGCT GGTGTGTCGA GCCAATGGGA ACCCGAAGCC GACCGTCCAG 
TGGCTGGTGA ATGGAGACCC TTTGCAATCG GCACCACCCA ACCCCAACCG TGAGGTGGCC 
GGAGACACTA TCATCTTCCG GGACACTCAG ATCAGCAGCA GGGCAGTGTA CCAGTGTAAC 
ACATCCAACG AACATGGCTA CCTGCTGGCC AATGCCTTCG TCAGCGTATT AGATGTACCC 
CCTCGGATGC TGTCTCCCCG GAACCAGCTC ATCAGGGTGA TCCTTTACAA CCGGACGCGA 
CTGGACTGTC CGTTCTTTGG GTCTCCCATT CCAACACTCC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGTGG TAACTACCAC GTCTACGAAA ATGGCAGCCT GGAAATCAAG 
ATGATTCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTGG CCACCAACAT CCTGGGAAAA 
GCTGAAAATC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACAG GATGCCTGAA 
GACCAGGTGG CCAAGAGGGG CACCACAGTG CAGCTGGAGT GCCGTGTGAA GCATGACCCC 
TCCTTGAAAC TCACAGTCTC CTGGCTGAAG GATGACGAGC CACTCTACAT TGGAAACAGG 
ATGAAAAAGG AAGATGACTC CCTGACCATC TTCGGAGTGG CAGAGCGGGA CCAAGGCAGT 
TACACGTGCA TGGCCAGCAC CGAGCTGGAC CAGGACCTGG CAAAGGCCTA CCTCACTGTT 
CTAGGGCGGC CAGACCGACC CAGGGACCTG GAGCTCACTG ACCTGGCGGA AAGGAGTGTG 
AGGCTGACCT GGATCCCGGG GGATGATAAC AACAGTCCCA TCACAGACTA CGTTGTCCAG 
TTTGAAGAGG ACCAGTTCCA GCCAGGAGTC TGGCATGACC ACTCCAAGTT CCCAGGCAGT 
GTCAACTCAG CCGTCCTCCA TCTGTCCCCG TATGTCAACT ATCAGTTCCG AGTCATCGCT 
GTCAACGAGG TTGGGAGCAG CCACCCCAGC CTTCCATCCG AGCGGTACCG AACAAGCGGG 
GCACCCCCTG AATCCAACCC CAGTGATGTG AAGGGCGAAG GGACACGAAA AAACAATATG 
GAGATCACAT GGACGCCTAT GAATGCTACC TCCGCCTTTG GCCCCAACCT GCGCTACATT 
GTCAAGTGGC GACGGAGAGA AACCCGAGAG ACTTGGAACA ATGTCACCGT GTGGGGCTCT 
CGCTATGTGG TGGGGCAGAC CCCTGTCTAC GTACCCTATG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAAGGCCC GGAGCCTGAA ACCGTCATTG GGTACTCGGG GGAAGATTAT 
CCCAGGGCTG CACCCACTGA AGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGCCTT 
CAGTGGAACC GCGTCTACCC TGACACGGTC CAGGGCCAGC TCAGAGAGTA TCGAGCTTAC 
TACTGGAGGG AAAGCAGTTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAGGCC 
AGCTTCCCTG GCGACCGCCC CCGGGGCGTG GTGGGTCGCC TGTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGAGA GGTGATGGGC CTCGAAGTGA AACCAAGGAA 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCAGAGTCCG ACAGCCCAAC 
CTGGAGACCA TCAACCTGGA ATGGGATCAC CCAGAGCACC CCAACGGGAT CCTGATTGGA 
TACACGCTCA GATACGTGCC CTTTAATGGA ACCAAACTGG GAAAGCAGAT GGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCTCGGTG CAGAGAGCAG ACCCCGTGTC CCGTTACCGC 
TTCTCCCTCA GTGCCAGGAC GCAGGTGGGC TCTGGAGAAG CAGCCACGGA GGAGTCCCCA 
ACACCTCCAA ATGAAGCTAC TCCAACTGCA GCTTACACCA ACAACCAGAC TGACATCGCC 
ACCCAGGGCT GGTTCATCGG GCTCATGTGT GCCATTGCCC TTCTGGTGCT GATCCTGCTG 
ATCGTCTGCT TCATCAAGAG GAGTCGTGGC GGCAAGTACC CAGTGCGAGA AAAGAAGGAT 
GTCCCCTTGG GCCCTGAAGA CCCCAAAGAA GAAGATGGTT CATTTGACTA CAGTGACGAG 
GACAACAAGC CCCTGCAGGG CAGCCAGACA TCTCTGGACG GCACCATCAA GCAGCAGGAG 
AGTGACGACA GCCTAGTGGA CTACGGCGAG GGTGGCGAGG GCCAGTTCAA CGAAGATGGC 
TCCTTTATTG GCCAGTACAC TGTCAGAAAG GACAAGGAGG AGACCGAGGG CAATGAGAGC 
TCAGAGGCCA CATCTCCAGT CAATGCCATC TATTCCCTGG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446361.1
CDS	309..3833
Translation	MARQQAPPWVHVALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWLVNGDPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPETVIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYPDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVGRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPTPPNEATPTAAYTNNQTDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVRKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	NM_053909.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurofascin (Nfasc), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053909.2

ORF Insert Sequence:

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACGTAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATTGAGAT TCCGATGGAT CTGACCCAAC CCCCAACGAT CACCAAGCAG 
TCGGTGAAGG ACCACATCGT GGACCCCCGA GATAACATCC TGATTGAATG TGAAGCTAAA 
GGGAACCCCG CCCCCAGTTT CCACTGGACT CGCAACAGCA GGTTCTTCAA CATTGCCAAG 
GACCCACGGG TGTCCATGAG GAGGAGGTCT GGGACCTTGG TGATCGACTT CCGCAGTGGT 
GGGCGGCCTG AGGAGTACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTCGGCACA 
GCTCTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC 
CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTAACCC TGCAGTGCAA CCCCCCACCT 
GGCCTCCCAT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC 
AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC 
ATGCAGACCG ACTACAGCTG CAATGCACGC TTCCACTTCA CCCACACCAT TCAGCAGAAG 
AATCCCTTCA CCCTCAAAGT CCTCACCAAC AACCCCTATA ATGACTCGTC TTTAAGAAAC 
CACCCTGACA TATATAGTGC CCGAGGAGTT GCGGAAAGAA CACCTAGCTT CATGTATCCC 
CAGGGCACGT CAAGCAGTCA GATGGTACTG CGCGGCATGG ACCTGCTGCT GGAGTGCATT 
GCCTCTGGGG TCCCAACACC AGATATTGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
GACAAGGCCA AGTTCGAGAA TTTTAACAAG GCTCTGCGCA TCACCAATGT CTCTGAAGAG 
GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC CCCATACTGG CTGGATGAGC CCAAGAACTT GATCCTGGCT 
CCTGGTGAAG ATGGGAGGCT GGTGTGTCGA GCCAATGGGA ACCCGAAGCC GACCGTCCAG 
TGGCTGGTGA ATGGAGACCC TTTGCAATCG GCACCACCCA ACCCCAACCG TGAGGTGGCC 
GGAGACACTA TCATCTTCCG GGACACTCAG ATCAGCAGCA GGGCAGTGTA CCAGTGTAAC 
ACATCCAACG AACATGGCTA CCTGCTGGCC AATGCCTTCG TCAGCGTATT AGATGTACCC 
CCTCGGATGC TGTCTCCCCG GAACCAGCTC ATCAGGGTGA TCCTTTACAA CCGGACGCGA 
CTGGACTGTC CGTTCTTTGG GTCTCCCATT CCAACACTCC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGTGG TAACTACCAC GTCTACGAAA ATGGCAGCCT GGAAATCAAG 
ATGATTCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTGG CCACCAACAT CCTGGGAAAA 
GCTGAAAATC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACAG GATGCCTGAA 
GACCAGGTGG CCAAGAGGGG CACCACAGTG CAGCTGGAGT GCCGTGTGAA GCATGACCCC 
TCCTTGAAAC TCACAGTCTC CTGGCTGAAG GATGACGAGC CACTCTACAT TGGAAACAGG 
ATGAAAAAGG AAGATGACTC CCTGACCATC TTCGGAGTGG CAGAGCGGGA CCAAGGCAGT 
TACACGTGCA TGGCCAGCAC CGAGCTGGAC CAGGACCTGG CAAAGGCCTA CCTCACTGTT 
CTAGGGCGGC CAGACCGACC CAGGGACCTG GAGCTCACTG ACCTGGCGGA AAGGAGTGTG 
AGGCTGACCT GGATCCCGGG GGATGATAAC AACAGTCCCA TCACAGACTA CGTTGTCCAG 
TTTGAAGAGG ACCAGTTCCA GCCAGGAGTC TGGCATGACC ACTCCAAGTT CCCAGGCAGT 
GTCAACTCAG CCGTCCTCCA TCTGTCCCCG TATGTCAACT ATCAGTTCCG AGTCATCGCT 
GTCAACGAGG TTGGGAGCAG CCACCCCAGC CTTCCATCCG AGCGGTACCG AACAAGCGGG 
GCACCCCCTG AATCCAACCC CAGTGATGTG AAGGGCGAAG GGACACGAAA AAACAATATG 
GAGATCACAT GGACGCCTAT GAATGCTACC TCCGCCTTTG GCCCCAACCT GCGCTACATT 
GTCAAGTGGC GACGGAGAGA AACCCGAGAG ACTTGGAACA ATGTCACCGT GTGGGGCTCT 
CGCTATGTGG TGGGGCAGAC CCCTGTCTAC GTACCCTATG AGATCCGAGT CCAGGCTGAA 
AATGACTTTG GGAAAGGCCC GGAGCCTGAA ACCGTCATTG GGTACTCGGG GGAAGATTAT 
CCCAGGGCTG CACCCACTGA AGTTAAAATC CGAGTCCTGA ACAGCACAGC CATCAGCCTT 
CAGTGGAACC GCGTCTACCC TGACACGGTC CAGGGCCAGC TCAGAGAGTA TCGAGCTTAC 
TACTGGAGGG AAAGCAGTTT GCTGAAGAAC CTGTGGGTGT CTCAGAAGAG ACAGCAGGCC 
AGCTTCCCTG GCGACCGCCC CCGGGGCGTG GTGGGTCGCC TGTTCCCCTA CAGTAACTAC 
AAGCTGGAGA TGGTTGTGGT CAATGGGAGA GGTGATGGGC CTCGAAGTGA AACCAAGGAA 
TTCACCACCC CGGAAGGAGT ACCCAGTGCC CCCAGGCGTT TCAGAGTCCG ACAGCCCAAC 
CTGGAGACCA TCAACCTGGA ATGGGATCAC CCAGAGCACC CCAACGGGAT CCTGATTGGA 
TACACGCTCA GATACGTGCC CTTTAATGGA ACCAAACTGG GAAAGCAGAT GGTGGAAAAC 
TTCTCTCCCA ATCAGACCAA GTTCTCGGTG CAGAGAGCAG ACCCCGTGTC CCGTTACCGC 
TTCTCCCTCA GTGCCAGGAC GCAGGTGGGC TCTGGAGAAG CAGCCACGGA GGAGTCCCCA 
ACACCTCCAA ATGAAGCTAC TCCAACTGCA GCTTACACCA ACAACCAGAC TGACATCGCC 
ACCCAGGGCT GGTTCATCGG GCTCATGTGT GCCATTGCCC TTCTGGTGCT GATCCTGCTG 
ATCGTCTGCT TCATCAAGAG GAGTCGTGGC GGCAAGTACC CAGTGCGAGA AAAGAAGGAT 
GTCCCCTTGG GCCCTGAAGA CCCCAAAGAA GAAGATGGTT CATTTGACTA CAGTGACGAG 
GACAACAAGC CCCTGCAGGG CAGCCAGACA TCTCTGGACG GCACCATCAA GCAGCAGGAG 
AGTGACGACA GCCTAGTGGA CTACGGCGAG GGTGGCGAGG GCCAGTTCAA CGAAGATGGC 
TCCTTTATTG GCCAGTACAC TGTCAGAAAG GACAAGGAGG AGACCGAGGG CAATGAGAGC 
TCAGAGGCCA CATCTCCAGT CAATGCCATC TATTCCCTGG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( NM_001160313.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001153785.1 Rattus norvegicus neurofascin (Nfasc), transcript variant 2, mRNA.

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CloneID	ORa12781
Clone ID Related Accession (Same CDS sequence)	NM_001160313.1
Accession Version	NM_001160313.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Rattus norvegicus(Norway rat)
Product	neurofascin isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY061639.1, U81035.1, AC105703.6 and BF410020.1. Transcript Variant: This variant (2) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. The promoter and 5' UTR for this variant have not been determined. The resulting isoform (2) includes the third fibronectin type 3 (FNIII) repeat, lacks the mucin-like domain, and has several differences near the N-terminus, compared to isoform 1. Isoform 2 is also known as NF155, as described by Davis et al. (PMID: 8947556). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U81035.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACGTAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATTGAGAT TCCGATGGAT CTGACCCAAC CCCCAACGAT CACCAAGCAG 
TCGGTGAAGG ACCACATCGT GGACCCCCGA GATAACATCC TGATTGAATG TGAAGCTAAA 
GGGAACCCCG CCCCCAGTTT CCACTGGACT CGCAACAGCA GGTTCTTCAA CATTGCCAAG 
GACCCACGGG TGTCCATGAG GAGGAGGTCT GGGACCTTGG TGATCGACTT CCGCAGTGGT 
GGGCGGCCTG AGGAGTACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTCGGCACA 
GCTCTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC 
CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTAACCC TGCAGTGCAA CCCCCCACCT 
GGCCTCCCAT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC 
AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC 
ATGCAGACCG ACTACAGCTG CAATGCACGC TTCCACTTCA CCCACACCAT TCAGCAGAAG 
AATCCCTTCA CCCTCAAAGT CCTCACCAAC AACCCCTATA ATGACTCGTC TTTAAGAAAC 
CACCCTGACA TATATAGTGC CCGAGGAGTT GCGGAAAGAA CACCTAGCTT CATGTATCCC 
CAGGGCACGT CAAGCAGTCA GATGGTACTG CGCGGCATGG ACCTGCTGCT GGAGTGCATT 
GCCTCTGGGG TCCCAACACC AGATATTGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
GACAAGGCCA AGTTCGAGAA TTTTAACAAG GCTCTGCGCA TCACCAATGT CTCTGAAGAG 
GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC CCCATACTGG CTGGATGAGC CCAAGAACTT GATCCTGGCT 
CCTGGTGAAG ATGGGAGGCT GGTGTGTCGA GCCAATGGGA ACCCGAAGCC GACCGTCCAG 
TGGCTGGTGA ATGGAGACCC TTTGCAATCG GCACCACCCA ACCCCAACCG TGAGGTGGCC 
GGAGACACTA TCATCTTCCG GGACACTCAG ATCAGCAGCA GGGCAGTGTA CCAGTGTAAC 
ACATCCAACG AACATGGCTA CCTGCTGGCC AATGCCTTCG TCAGCGTATT AGATGTACCC 
CCTCGGATGC TGTCTCCCCG GAACCAGCTC ATCAGGGTGA TCCTTTACAA CCGGACGCGA 
CTGGACTGTC CGTTCTTTGG GTCTCCCATT CCAACACTCC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGTGG TAACTACCAC GTCTACGAAA ATGGCAGCCT GGAAATCAAG 
ATGATTCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTGG CCACCAACAT CCTGGGAAAA 
GCTGAAAATC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACAG GATGCCTGAA 
GACCAGGTGG CCAAGAGGGG CACCACAGTG CAGCTGGAGT GCCGTGTGAA GCATGACCCC 
TCCTTGAAAC TCACAGTCTC CTGGCTGAAG GATGACGAGC CACTCTACAT TGGAAACAGG 
ATGAAAAAGG AAGATGACTC CCTGACCATC TTCGGAGTGG CAGAGCGGGA CCAAGGCAGT 
TACACGTGCA TGGCCAGCAC CGAGCTGGAC CAGGACCTGG CAAAGGCCTA CCTCACTGTT 
CTAGCTGATC AGGCCACTCC GACTAACCGT TTGGCTGCCC TGCCCAAAGG GCGGCCAGAC 
CGACCCAGGG ACCTGGAGCT CACTGACCTG GCGGAAAGGA GTGTGAGGCT GACCTGGATC 
CCGGGGGATG ATAACAACAG TCCCATCACA GACTACGTTG TCCAGTTTGA AGAGGACCAG 
TTCCAGCCAG GAGTCTGGCA TGACCACTCC AAGTTCCCAG GCAGTGTCAA CTCAGCCGTC 
CTCCATCTGT CCCCGTATGT CAACTATCAG TTCCGAGTCA TCGCTGTCAA CGAGGTTGGG 
AGCAGCCACC CCAGCCTTCC ATCCGAGCGG TACCGAACAA GCGGGGCACC CCCTGAATCC 
AACCCCAGTG ATGTGAAGGG CGAAGGGACA CGAAAAAACA ATATGGAGAT CACATGGACG 
CCTATGAATG CTACCTCCGC CTTTGGCCCC AACCTGCGCT ACATTGTCAA GTGGCGACGG 
AGAGAAACCC GAGAGACTTG GAACAATGTC ACCGTGTGGG GCTCTCGCTA TGTGGTGGGG 
CAGACCCCTG TCTACGTACC CTATGAGATC CGAGTCCAGG CTGAAAATGA CTTTGGGAAA 
GGCCCGGAGC CTGAAACCGT CATTGGGTAC TCGGGGGAAG ATTATCCCAG GGCTGCACCC 
ACTGAAGTTA AAATCCGAGT CCTGAACAGC ACAGCCATCA GCCTTCAGTG GAACCGCGTC 
TACCCTGACA CGGTCCAGGG CCAGCTCAGA GAGTATCGAG CTTACTACTG GAGGGAAAGC 
AGTTTGCTGA AGAACCTGTG GGTGTCTCAG AAGAGACAGC AGGCCAGCTT CCCTGGCGAC 
CGCCCCCGGG GCGTGGTGGG TCGCCTGTTC CCCTACAGTA ACTACAAGCT GGAGATGGTT 
GTGGTCAATG GGAGAGGTGA TGGGCCTCGA AGTGAAACCA AGGAATTCAC CACCCCGGAA 
GGAGTACCCA GTGCCCCCAG GCGTTTCAGA GTCCGACAGC CCAACCTGGA GACCATCAAC 
CTGGAATGGG ATCACCCAGA GCACCCCAAC GGGATCCTGA TTGGATACAC GCTCAGATAC 
GTGCCCTTTA ATGGAACCAA ACTGGGAAAG CAGATGGTGG AAAACTTCTC TCCCAATCAG 
ACCAAGTTCT CGGTGCAGAG AGCAGACCCC GTGTCCCGTT ACCGCTTCTC CCTCAGTGCC 
AGGACGCAGG TGGGCTCTGG AGAAGCAGCC ACGGAGGAGT CCCCAACACC TCCAAATGAA 
GCTACTCCAA CTGCAGCTTA CACCAACAAC CAGACTGACA TCGCCACCCA GGGCTGGTTC 
ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG GTGCTGATCC TGCTGATCGT CTGCTTCATC 
AAGAGGAGTC GTGGCGGCAA GTACCCAGTG CGAGAAAAGA AGGATGTCCC CTTGGGCCCT 
GAAGACCCCA AAGAAGAAGA TGGTTCATTT GACTACAGTG ACGAGGACAA CAAGCCCCTG 
CAGGGCAGCC AGACATCTCT GGACGGCACC ATCAAGCAGC AGGAGAGTGA CGACAGCCTA 
GTGGACTACG GCGAGGGTGG CGAGGGCCAG TTCAACGAAG ATGGCTCCTT TATTGGCCAG 
TACACTGTCA GAAAGGACAA GGAGGAGACC GAGGGCAATG AGAGCTCAGA GGCCACATCT 
CCAGTCAATG CCATCTATTC CCTGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153785.1
CDS	91..3660
Translation	MARQQAPPWVHVALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWLVNGDPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPETVIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYPDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVGRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPTPPNEATPTAAYTNNQTDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVRKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	NM_001160313.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurofascin (Nfasc), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160313.1

ORF Insert Sequence:

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACGTAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATTGAGAT TCCGATGGAT CTGACCCAAC CCCCAACGAT CACCAAGCAG 
TCGGTGAAGG ACCACATCGT GGACCCCCGA GATAACATCC TGATTGAATG TGAAGCTAAA 
GGGAACCCCG CCCCCAGTTT CCACTGGACT CGCAACAGCA GGTTCTTCAA CATTGCCAAG 
GACCCACGGG TGTCCATGAG GAGGAGGTCT GGGACCTTGG TGATCGACTT CCGCAGTGGT 
GGGCGGCCTG AGGAGTACGA AGGGGAGTAC CAGTGCTTTG CCCGGAACAA ATTCGGCACA 
GCTCTTAGCA ACCGCATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC 
CTAGACCCCG TCGTGGTTCA AGAGGGTGCC CCCTTAACCC TGCAGTGCAA CCCCCCACCT 
GGCCTCCCAT CCCCCGTCAT CTTCTGGATG AGCAGCTCCA TGGAGCCCAT CACCCAGGAC 
AAGCGTGTCT CCCAGGGTCA CAACGGGGAC CTGTACTTCT CCAACGTCAT GCTGCAGGAC 
ATGCAGACCG ACTACAGCTG CAATGCACGC TTCCACTTCA CCCACACCAT TCAGCAGAAG 
AATCCCTTCA CCCTCAAAGT CCTCACCAAC AACCCCTATA ATGACTCGTC TTTAAGAAAC 
CACCCTGACA TATATAGTGC CCGAGGAGTT GCGGAAAGAA CACCTAGCTT CATGTATCCC 
CAGGGCACGT CAAGCAGTCA GATGGTACTG CGCGGCATGG ACCTGCTGCT GGAGTGCATT 
GCCTCTGGGG TCCCAACACC AGATATTGCA TGGTACAAGA AAGGTGGGGA CCTCCCATCT 
GACAAGGCCA AGTTCGAGAA TTTTAACAAG GCTCTGCGCA TCACCAATGT CTCTGAAGAG 
GACTCTGGGG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC 
TCGGTGAGAG TAAAGGCTGC CCCATACTGG CTGGATGAGC CCAAGAACTT GATCCTGGCT 
CCTGGTGAAG ATGGGAGGCT GGTGTGTCGA GCCAATGGGA ACCCGAAGCC GACCGTCCAG 
TGGCTGGTGA ATGGAGACCC TTTGCAATCG GCACCACCCA ACCCCAACCG TGAGGTGGCC 
GGAGACACTA TCATCTTCCG GGACACTCAG ATCAGCAGCA GGGCAGTGTA CCAGTGTAAC 
ACATCCAACG AACATGGCTA CCTGCTGGCC AATGCCTTCG TCAGCGTATT AGATGTACCC 
CCTCGGATGC TGTCTCCCCG GAACCAGCTC ATCAGGGTGA TCCTTTACAA CCGGACGCGA 
CTGGACTGTC CGTTCTTTGG GTCTCCCATT CCAACACTCC GATGGTTTAA GAATGGGCAA 
GGAAGCAACC TGGATGGTGG TAACTACCAC GTCTACGAAA ATGGCAGCCT GGAAATCAAG 
ATGATTCGCA AAGAGGACCA AGGCATCTAC ACCTGTGTGG CCACCAACAT CCTGGGAAAA 
GCTGAAAATC AAGTCCGCCT GGAGGTCAAA GACCCCACCA GGATCTACAG GATGCCTGAA 
GACCAGGTGG CCAAGAGGGG CACCACAGTG CAGCTGGAGT GCCGTGTGAA GCATGACCCC 
TCCTTGAAAC TCACAGTCTC CTGGCTGAAG GATGACGAGC CACTCTACAT TGGAAACAGG 
ATGAAAAAGG AAGATGACTC CCTGACCATC TTCGGAGTGG CAGAGCGGGA CCAAGGCAGT 
TACACGTGCA TGGCCAGCAC CGAGCTGGAC CAGGACCTGG CAAAGGCCTA CCTCACTGTT 
CTAGCTGATC AGGCCACTCC GACTAACCGT TTGGCTGCCC TGCCCAAAGG GCGGCCAGAC 
CGACCCAGGG ACCTGGAGCT CACTGACCTG GCGGAAAGGA GTGTGAGGCT GACCTGGATC 
CCGGGGGATG ATAACAACAG TCCCATCACA GACTACGTTG TCCAGTTTGA AGAGGACCAG 
TTCCAGCCAG GAGTCTGGCA TGACCACTCC AAGTTCCCAG GCAGTGTCAA CTCAGCCGTC 
CTCCATCTGT CCCCGTATGT CAACTATCAG TTCCGAGTCA TCGCTGTCAA CGAGGTTGGG 
AGCAGCCACC CCAGCCTTCC ATCCGAGCGG TACCGAACAA GCGGGGCACC CCCTGAATCC 
AACCCCAGTG ATGTGAAGGG CGAAGGGACA CGAAAAAACA ATATGGAGAT CACATGGACG 
CCTATGAATG CTACCTCCGC CTTTGGCCCC AACCTGCGCT ACATTGTCAA GTGGCGACGG 
AGAGAAACCC GAGAGACTTG GAACAATGTC ACCGTGTGGG GCTCTCGCTA TGTGGTGGGG 
CAGACCCCTG TCTACGTACC CTATGAGATC CGAGTCCAGG CTGAAAATGA CTTTGGGAAA 
GGCCCGGAGC CTGAAACCGT CATTGGGTAC TCGGGGGAAG ATTATCCCAG GGCTGCACCC 
ACTGAAGTTA AAATCCGAGT CCTGAACAGC ACAGCCATCA GCCTTCAGTG GAACCGCGTC 
TACCCTGACA CGGTCCAGGG CCAGCTCAGA GAGTATCGAG CTTACTACTG GAGGGAAAGC 
AGTTTGCTGA AGAACCTGTG GGTGTCTCAG AAGAGACAGC AGGCCAGCTT CCCTGGCGAC 
CGCCCCCGGG GCGTGGTGGG TCGCCTGTTC CCCTACAGTA ACTACAAGCT GGAGATGGTT 
GTGGTCAATG GGAGAGGTGA TGGGCCTCGA AGTGAAACCA AGGAATTCAC CACCCCGGAA 
GGAGTACCCA GTGCCCCCAG GCGTTTCAGA GTCCGACAGC CCAACCTGGA GACCATCAAC 
CTGGAATGGG ATCACCCAGA GCACCCCAAC GGGATCCTGA TTGGATACAC GCTCAGATAC 
GTGCCCTTTA ATGGAACCAA ACTGGGAAAG CAGATGGTGG AAAACTTCTC TCCCAATCAG 
ACCAAGTTCT CGGTGCAGAG AGCAGACCCC GTGTCCCGTT ACCGCTTCTC CCTCAGTGCC 
AGGACGCAGG TGGGCTCTGG AGAAGCAGCC ACGGAGGAGT CCCCAACACC TCCAAATGAA 
GCTACTCCAA CTGCAGCTTA CACCAACAAC CAGACTGACA TCGCCACCCA GGGCTGGTTC 
ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG GTGCTGATCC TGCTGATCGT CTGCTTCATC 
AAGAGGAGTC GTGGCGGCAA GTACCCAGTG CGAGAAAAGA AGGATGTCCC CTTGGGCCCT 
GAAGACCCCA AAGAAGAAGA TGGTTCATTT GACTACAGTG ACGAGGACAA CAAGCCCCTG 
CAGGGCAGCC AGACATCTCT GGACGGCACC ATCAAGCAGC AGGAGAGTGA CGACAGCCTA 
GTGGACTACG GCGAGGGTGG CGAGGGCCAG TTCAACGAAG ATGGCTCCTT TATTGGCCAG 
TACACTGTCA GAAAGGACAA GGAGGAGACC GAGGGCAATG AGAGCTCAGA GGCCACATCT 
CCAGTCAATG CCATCTATTC CCTGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_006249738.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249800.1 Rattus norvegicus neurofascin (Nfasc), transcript variant X14, mRNA.

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CloneID	ORa35282
Clone ID Related Accession (Same CDS sequence)	XM_006249738.3
Accession Version	XM_006249738.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neurofascin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249738.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA 
TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA 
GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG 
AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG 
GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC 
CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCTGAC CCAACCCCCA 
ACGATCACCA AGCAGTCGGT GAAGGACCAC ATCGTGGACC CCCGAGATAA CATCCTGATT 
GAATGTGAAG CTAAAGGGAA CCCCGCCCCC AGTTTCCACT GGACTCGCAA CAGCAGGTTC 
TTCAACATTG CCAAGGACCC ACGGGTGTCC ATGAGGAGGA GGTCTGGGAC CTTGGTGATC 
GACTTCCGCA GTGGTGGGCG GCCTGAGGAG TACGAAGGGG AGTACCAGTG CTTTGCCCGG 
AACAAATTCG GCACAGCTCT TAGCAACCGC ATCCGCCTGC AGGTGTCCAA ATCTCCCCTG 
TGGCCCAAGG AAAACCTAGA CCCCGTCGTG GTTCAAGAGG GTGCCCCCTT AACCCTGCAG 
TGCAACCCCC CACCTGGCCT CCCATCCCCC GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AGGACAAGCG TGTCTCCCAG GGTCACAACG GGGACCTGTA CTTCTCCAAC 
GTCATGCTGC AGGACATGCA GACCGACTAC AGCTGCAATG CACGCTTCCA CTTCACCCAC 
ACCATTCAGC AGAAGAATCC CTTCACCCTC AAAGTCCTCA CCAACAACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATATAT AGTGCCCGAG GAGTTGCGGA AAGAACACCT 
AGCTTCATGT ATCCCCAGGG CACGTCAAGC AGTCAGATGG TACTGCGCGG CATGGACCTG 
CTGCTGGAGT GCATTGCCTC TGGGGTCCCA ACACCAGATA TTGCATGGTA CAAGAAAGGT 
GGGGACCTCC CATCTGACAA GGCCAAGTTC GAGAATTTTA ACAAGGCTCT GCGCATCACC 
AATGTCTCTG AAGAGGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGC 
ATCCGGCACA CGATCTCGGT GAGAGTAAAG GCTGCCCCAT ACTGGCTGGA TGAGCCCAAG 
AACTTGATCC TGGCTCCTGG TGAAGATGGG AGGCTGGTGT GTCGAGCCAA TGGGAACCCG 
AAGCCGACCG TCCAGTGGCT GGTGAATGGA GACCCTTTGC AATCGGCACC ACCCAACCCC 
AACCGTGAGG TGGCCGGAGA CACTATCATC TTCCGGGACA CTCAGATCAG CAGCAGGGCA 
GTGTACCAGT GTAACACATC CAACGAACAT GGCTACCTGC TGGCCAATGC CTTCGTCAGC 
GTATTAGATG TACCCCCTCG GATGCTGTCT CCCCGGAACC AGCTCATCAG GGTGATCCTT 
TACAACCGGA CGCGACTGGA CTGTCCGTTC TTTGGGTCTC CCATTCCAAC ACTCCGATGG 
TTTAAGAATG GGCAAGGAAG CAACCTGGAT GGTGGTAACT ACCACGTCTA CGAAAATGGC 
AGCCTGGAAA TCAAGATGAT TCGCAAAGAG GACCAAGGCA TCTACACCTG TGTGGCCACC 
AACATCCTGG GAAAAGCTGA AAATCAAGTC CGCCTGGAGG TCAAAGACCC CACCAGGATC 
TACAGGATGC CTGAAGACCA GGTGGCCAAG AGGGGCACCA CAGTGCAGCT GGAGTGCCGT 
GTGAAGCATG ACCCCTCCTT GAAACTCACA GTCTCCTGGC TGAAGGATGA CGAGCCACTC 
TACATTGGAA ACAGGATGAA AAAGGAAGAT GACTCCCTGA CCATCTTCGG AGTGGCAGAG 
CGGGACCAAG GCAGTTACAC GTGCATGGCC AGCACCGAGC TGGACCAGGA CCTGGCAAAG 
GCCTACCTCA CTGTTCTAGG GCGGCCAGAC CGACCCAGGG ACCTGGAGCT CACTGACCTG 
GCGGAAAGGA GTGTGAGGCT GACCTGGATC CCGGGGGATG ATAACAACAG TCCCATCACA 
GACTACGTTG TCCAGTTTGA AGAGGACCAG TTCCAGCCAG GAGTCTGGCA TGACCACTCC 
AAGTTCCCAG GCAGTGTCAA CTCAGCCGTC CTCCATCTGT CCCCGTATGT CAACTATCAG 
TTCCGAGTCA TCGCTGTCAA CGAGGTTGGG AGCAGCCACC CCAGCCTTCC ATCCGAGCGG 
TACCGAACAA GCGGGGCACC CCCTGAATCC AACCCCAGTG ATGTGAAGGG CGAAGGGACA 
CGAAAAAACA ATATGGAGAT CACATGGACG CCTATGAATG CTACCTCCGC CTTTGGCCCC 
AACCTGCGCT ACATTGTCAA GTGGCGACGG AGAGAAACCC GAGAGACTTG GAACAATGTC 
ACCGTGTGGG GCTCTCGCTA TGTGGTGGGG CAGACCCCTG TCTACGTACC CTATGAGATC 
CGAGTCCAGG CTGAAAATGA CTTTGGGAAA GGCCCGGAGC CTGAAACCGT CATTGGGTAC 
TCGGGGGAAG ATTATCCCAG GGCTGCACCC ACTGAAGTTA AAATCCGAGT CCTGAACAGC 
ACAGCCATCA GCCTTCAGTG GAACCGCGTC TACTCTGACA CGGTCCAGGG CCAGCTCAGA 
GAGTATCGAG CTTACTACTG GAGGGAAAGC AGTTTGCTGA AGAACCTGTG GGTGTCTCAG 
AAGAGACAGC AGGCCAGCTT CCCTGGCGAC CGCCCCCGGG GCGTGGTGGG TCGCCTGTTC 
CCCTACAGTA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGAGAGGTGA TGGGCCTCGA 
AGTGAAACCA AGGAATTCAC CACCCCGGAA GGAGTACCCA GTGCCCCCAG GCGTTTCAGA 
GTCCGACAGC CCAACCTGGA GACCATCAAC CTGGAATGGG ATCACCCAGA GCACCCCAAC 
GGGATCCTGA TTGGATACAC GCTCAGATAC GTGCCCTTTA ATGGAACCAA ACTGGGAAAG 
CAGATGGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCT CGGTGCAGAG AGCAGACCCC 
GTGTCCCGTT ACCGCTTCTC CCTCAGTGCC AGGACGCAGG TGGGCTCTGG AGAAGCAGCC 
ACGGAGGAGT CCCCAACACC TCCAAATGAA GCTACTCCAA CTGCAGCTTA CACCAACAAC 
CAGACTGACA TCGCCACCCA GGGCTGGTTC ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG 
GTGCTGATCC TGCTTATCGT CTGCTTCATC AAGAGGAGTC GTGGCGGCAA GTACCCAGTG 
CGAGAAAAGA AGGATGTCCC CTTGGGCCCT GAAGACCCCA AAGAAGAAGA TGGTTCATTT 
GACTACAGTG ACGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACATCTCT GGACGGCACC 
ATCAAGCAGC AGGAGAGTGA CGACAGCCTA GTGGACTACG GCGAGGGTGG CGAGGGCCAG 
TTCAACGAAG ATGGCTCCTT TATTGGCCAG TACACTGTCA GAAAGGACAA GGAGGAGACC 
GAGGGCAATG AGAGCTCAGA GGCCACATCT CCAGTCAATG CCATCTATTC CCTGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249800.1
CDS	51..3890
Translation	MRRLAARAAAGDAGEVAAPGADIWDCAQGWSWSSWRLTDLCAIWEPGVFLPSAAAVNRSRASAGVAKSRPNEAEKLSTVWKAHEERRKASRARGLGSAEQGLALRMARQQAPPWVHVALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWLVNGDPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPETVIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVGRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPTPPNEATPTAAYTNNQTDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVRKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_006249738.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurofascin (Nfasc), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249738.3

ORF Insert Sequence:

ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA 
TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA 
GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG 
AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG 
GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC 
CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCTGAC CCAACCCCCA 
ACGATCACCA AGCAGTCGGT GAAGGACCAC ATCGTGGACC CCCGAGATAA CATCCTGATT 
GAATGTGAAG CTAAAGGGAA CCCCGCCCCC AGTTTCCACT GGACTCGCAA CAGCAGGTTC 
TTCAACATTG CCAAGGACCC ACGGGTGTCC ATGAGGAGGA GGTCTGGGAC CTTGGTGATC 
GACTTCCGCA GTGGTGGGCG GCCTGAGGAG TACGAAGGGG AGTACCAGTG CTTTGCCCGG 
AACAAATTCG GCACAGCTCT TAGCAACCGC ATCCGCCTGC AGGTGTCCAA ATCTCCCCTG 
TGGCCCAAGG AAAACCTAGA CCCCGTCGTG GTTCAAGAGG GTGCCCCCTT AACCCTGCAG 
TGCAACCCCC CACCTGGCCT CCCATCCCCC GTCATCTTCT GGATGAGCAG CTCCATGGAG 
CCCATCACCC AGGACAAGCG TGTCTCCCAG GGTCACAACG GGGACCTGTA CTTCTCCAAC 
GTCATGCTGC AGGACATGCA GACCGACTAC AGCTGCAATG CACGCTTCCA CTTCACCCAC 
ACCATTCAGC AGAAGAATCC CTTCACCCTC AAAGTCCTCA CCAACAACCC CTATAATGAC 
TCGTCCTTAA GAAACCACCC TGACATATAT AGTGCCCGAG GAGTTGCGGA AAGAACACCT 
AGCTTCATGT ATCCCCAGGG CACGTCAAGC AGTCAGATGG TACTGCGCGG CATGGACCTG 
CTGCTGGAGT GCATTGCCTC TGGGGTCCCA ACACCAGATA TTGCATGGTA CAAGAAAGGT 
GGGGACCTCC CATCTGACAA GGCCAAGTTC GAGAATTTTA ACAAGGCTCT GCGCATCACC 
AATGTCTCTG AAGAGGACTC TGGGGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGC 
ATCCGGCACA CGATCTCGGT GAGAGTAAAG GCTGCCCCAT ACTGGCTGGA TGAGCCCAAG 
AACTTGATCC TGGCTCCTGG TGAAGATGGG AGGCTGGTGT GTCGAGCCAA TGGGAACCCG 
AAGCCGACCG TCCAGTGGCT GGTGAATGGA GACCCTTTGC AATCGGCACC ACCCAACCCC 
AACCGTGAGG TGGCCGGAGA CACTATCATC TTCCGGGACA CTCAGATCAG CAGCAGGGCA 
GTGTACCAGT GTAACACATC CAACGAACAT GGCTACCTGC TGGCCAATGC CTTCGTCAGC 
GTATTAGATG TACCCCCTCG GATGCTGTCT CCCCGGAACC AGCTCATCAG GGTGATCCTT 
TACAACCGGA CGCGACTGGA CTGTCCGTTC TTTGGGTCTC CCATTCCAAC ACTCCGATGG 
TTTAAGAATG GGCAAGGAAG CAACCTGGAT GGTGGTAACT ACCACGTCTA CGAAAATGGC 
AGCCTGGAAA TCAAGATGAT TCGCAAAGAG GACCAAGGCA TCTACACCTG TGTGGCCACC 
AACATCCTGG GAAAAGCTGA AAATCAAGTC CGCCTGGAGG TCAAAGACCC CACCAGGATC 
TACAGGATGC CTGAAGACCA GGTGGCCAAG AGGGGCACCA CAGTGCAGCT GGAGTGCCGT 
GTGAAGCATG ACCCCTCCTT GAAACTCACA GTCTCCTGGC TGAAGGATGA CGAGCCACTC 
TACATTGGAA ACAGGATGAA AAAGGAAGAT GACTCCCTGA CCATCTTCGG AGTGGCAGAG 
CGGGACCAAG GCAGTTACAC GTGCATGGCC AGCACCGAGC TGGACCAGGA CCTGGCAAAG 
GCCTACCTCA CTGTTCTAGG GCGGCCAGAC CGACCCAGGG ACCTGGAGCT CACTGACCTG 
GCGGAAAGGA GTGTGAGGCT GACCTGGATC CCGGGGGATG ATAACAACAG TCCCATCACA 
GACTACGTTG TCCAGTTTGA AGAGGACCAG TTCCAGCCAG GAGTCTGGCA TGACCACTCC 
AAGTTCCCAG GCAGTGTCAA CTCAGCCGTC CTCCATCTGT CCCCGTATGT CAACTATCAG 
TTCCGAGTCA TCGCTGTCAA CGAGGTTGGG AGCAGCCACC CCAGCCTTCC ATCCGAGCGG 
TACCGAACAA GCGGGGCACC CCCTGAATCC AACCCCAGTG ATGTGAAGGG CGAAGGGACA 
CGAAAAAACA ATATGGAGAT CACATGGACG CCTATGAATG CTACCTCCGC CTTTGGCCCC 
AACCTGCGCT ACATTGTCAA GTGGCGACGG AGAGAAACCC GAGAGACTTG GAACAATGTC 
ACCGTGTGGG GCTCTCGCTA TGTGGTGGGG CAGACCCCTG TCTACGTACC CTATGAGATC 
CGAGTCCAGG CTGAAAATGA CTTTGGGAAA GGCCCGGAGC CTGAAACCGT CATTGGGTAC 
TCGGGGGAAG ATTATCCCAG GGCTGCACCC ACTGAAGTTA AAATCCGAGT CCTGAACAGC 
ACAGCCATCA GCCTTCAGTG GAACCGCGTC TACTCTGACA CGGTCCAGGG CCAGCTCAGA 
GAGTATCGAG CTTACTACTG GAGGGAAAGC AGTTTGCTGA AGAACCTGTG GGTGTCTCAG 
AAGAGACAGC AGGCCAGCTT CCCTGGCGAC CGCCCCCGGG GCGTGGTGGG TCGCCTGTTC 
CCCTACAGTA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGAGAGGTGA TGGGCCTCGA 
AGTGAAACCA AGGAATTCAC CACCCCGGAA GGAGTACCCA GTGCCCCCAG GCGTTTCAGA 
GTCCGACAGC CCAACCTGGA GACCATCAAC CTGGAATGGG ATCACCCAGA GCACCCCAAC 
GGGATCCTGA TTGGATACAC GCTCAGATAC GTGCCCTTTA ATGGAACCAA ACTGGGAAAG 
CAGATGGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCT CGGTGCAGAG AGCAGACCCC 
GTGTCCCGTT ACCGCTTCTC CCTCAGTGCC AGGACGCAGG TGGGCTCTGG AGAAGCAGCC 
ACGGAGGAGT CCCCAACACC TCCAAATGAA GCTACTCCAA CTGCAGCTTA CACCAACAAC 
CAGACTGACA TCGCCACCCA GGGCTGGTTC ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG 
GTGCTGATCC TGCTTATCGT CTGCTTCATC AAGAGGAGTC GTGGCGGCAA GTACCCAGTG 
CGAGAAAAGA AGGATGTCCC CTTGGGCCCT GAAGACCCCA AAGAAGAAGA TGGTTCATTT 
GACTACAGTG ACGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACATCTCT GGACGGCACC 
ATCAAGCAGC AGGAGAGTGA CGACAGCCTA GTGGACTACG GCGAGGGTGG CGAGGGCCAG 
TTCAACGAAG ATGGCTCCTT TATTGGCCAG TACACTGTCA GAAAGGACAA GGAGGAGACC 
GAGGGCAATG AGAGCTCAGA GGCCACATCT CCAGTCAATG CCATCTATTC CCTGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_017598638.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454127.1 Rattus norvegicus neurofascin (Nfasc), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa35271
Clone ID Related Accession (Same CDS sequence)	XM_017598638.1 , XM_006249727.3
Accession Version	XM_017598638.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neurofascin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA 
TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA 
GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG 
AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG 
GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC 
CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT 
GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC 
CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG 
ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG 
TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG 
TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG 
GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT 
GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG 
GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA 
CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC 
AACAACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATATATAG TGCCCGAGGA 
GTTGCGGAAA GAACACCTAG CTTCATGTAT CCCCAGGGCA CGTCAAGCAG TCAGATGGTA 
CTGCGCGGCA TGGACCTGCT GCTGGAGTGC ATTGCCTCTG GGGTCCCAAC ACCAGATATT 
GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTCGA GAATTTTAAC 
AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCCCCATAC 
TGGCTGGATG AGCCCAAGAA CTTGATCCTG GCTCCTGGTG AAGATGGGAG GCTGGTGTGT 
CGAGCCAATG GGAACCCGAA GCCGACCGTC CAGTGGCTGG TGAATGGAGA CCCTTTGCAA 
TCGGCACCAC CCAACCCCAA CCGTGAGGTG GCCGGAGACA CTATCATCTT CCGGGACACT 
CAGATCAGCA GCAGGGCAGT GTACCAGTGT AACACATCCA ACGAACATGG CTACCTGCTG 
GCCAATGCCT TCGTCAGCGT ATTAGATGTA CCCCCTCGGA TGCTGTCTCC CCGGAACCAG 
CTCATCAGGG TGATCCTTTA CAACCGGACG CGACTGGACT GTCCGTTCTT TGGGTCTCCC 
ATTCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG TGGTAACTAC 
CACGTCTACG AAAATGGCAG CCTGGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC 
TACACCTGTG TGGCCACCAA CATCCTGGGA AAAGCTGAAA ATCAAGTCCG CCTGGAGGTC 
AAAGACCCCA CCAGGATCTA CAGGATGCCT GAAGACCAGG TGGCCAAGAG GGGCACCACA 
GTGCAGCTGG AGTGCCGTGT GAAGCATGAC CCCTCCTTGA AACTCACAGT CTCCTGGCTG 
AAGGATGACG AGCCACTCTA CATTGGAAAC AGGATGAAAA AGGAAGATGA CTCCCTGACC 
ATCTTCGGAG TGGCAGAGCG GGACCAAGGC AGTTACACGT GCATGGCCAG CACCGAGCTG 
GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCGACTAAC 
CGTTTGGCTG CCCTGCCCAA AGGGCGGCCA GACCGACCCA GGGACCTGGA GCTCACTGAC 
CTGGCGGAAA GGAGTGTGAG GCTGACCTGG ATCCCGGGGG ATGATAACAA CAGTCCCATC 
ACAGACTACG TTGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGAGTCTG GCATGACCAC 
TCCAAGTTCC CAGGCAGTGT CAACTCAGCC GTCCTCCATC TGTCCCCGTA TGTCAACTAT 
CAGTTCCGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG 
CGGTACCGAA CAAGCGGGGC ACCCCCTGAA TCCAACCCCA GTGATGTGAA GGGCGAAGGG 
ACACGAAAAA ACAATATGGA GATCACATGG ACGCCTATGA ATGCTACCTC CGCCTTTGGC 
CCCAACCTGC GCTACATTGT CAAGTGGCGA CGGAGAGAAA CCCGAGAGAC TTGGAACAAT 
GTCACCGTGT GGGGCTCTCG CTATGTGGTG GGGCAGACCC CTGTCTACGT ACCCTATGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCGG AGCCTGAAAC CGTCATTGGG 
TACTCGGGGG AAGATTATCC CAGGGCTGCA CCCACTGAAG TTAAAATCCG AGTCCTGAAC 
AGCACAGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCTG ACACGGTCCA GGGCCAGCTC 
AGAGAGTATC GAGCTTACTA CTGGAGGGAA AGCAGTTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGAC AGCAGGCCAG CTTCCCTGGC GACCGCCCCC GGGGCGTGGT GGGTCGCCTG 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG TGATGGGCCT 
CGAAGTGAAA CCAAGGAATT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
AGAGTCCGAC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGATCACCC AGAGCACCCC 
AACGGGATCC TGATTGGATA CACGCTCAGA TACGTGCCCT TTAATGGAAC CAAACTGGGA 
AAGCAGATGG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCTCGGTGCA GAGAGCAGAC 
CCCGTGTCCC GTTACCGCTT CTCCCTCAGT GCCAGGACGC AGGTGGGCTC TGGAGAAGCA 
GCCACGGAGG AGTCCCCAAC ACCTCCAAAT GAAGCTACTC CAACTGCAGC TCCTCCCACA 
TTGCCCCCGA CTACCGTGGG TACCACAGGC CTTGTGAGCA GTACTGATGC TACTGCCCTT 
GCTGCCACCA GTGAAGCCAC AACAGTTCCC ATCATCCCAA CCGTCGTACC TACCACCGTC 
GCCACCACCA TTGCCACAAC TACTACAACC ACTGCCGCCG CCACCACCAC TACCACCACG 
GAGAGTCCTC CCACTACCAC CACTGGGACT AAGATTCACG AAACCGCCCC CGACGAGCAG 
TCCATTTGGA ACGTCACGGT GCTCCCCAAC AGTAAATGGG CCAACATCAC CTGGAAGCAC 
AATTTCAGGC CTGGAACTGA CTTTGTGGTT GAGTACATCG ACAGCAACCA TACGAAAAAA 
ACTGTCCCTG TTAAGGCCCA AGCCCAGCCC ATACAGCTGA CAGACCTCTT TCCCGGGATG 
ACGTACACGT TGCGGGTGTA TTCCCGGGAC AACGAGGGCA TCAGCAGTAC CGTCATCACC 
TTTATGACCA GTACAGCTTA CACCAACAAC CAGACTGACA TCGCCACCCA GGGCTGGTTC 
ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG GTGCTGATCC TGCTTATCGT CTGCTTCATC 
AAGAGGAGTC GTGGCGGCAA GTACCCAGTG CGAGAAAAGA AGGATGTCCC CTTGGGCCCT 
GAAGACCCCA AAGAAGAAGA TGGTTCATTT GACTACAGTG ACGAGGACAA CAAGCCCCTG 
CAGGGCAGCC AGACATCTCT GGACGGCACC ATCAAGCAGC AGGAGAGTGA CGACAGCCTA 
GTGGACTACG GCGAGGGTGG CGAGGGCCAG TTCAACGAAG ATGGCTCCTT TATTGGCCAG 
TACACTGTCA GAAAGGACAA GGAGGAGACC GAGGGCAATG AGAGCTCAGA GGCCACATCT 
CCAGTCAATG CCATCTATTC CCTGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454127.1
CDS	53..4462
Translation	MRRLAARAAAGDAGEVAAPGADIWDCAQGWSWSSWRLTDLCAIWEPGVFLPSAAAVNRSRASAGVAKSRPNEAEKLSTVWKAHEERRKASRARGLGSAEQGLALRMARQQAPPWVHVALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWLVNGDPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPETVIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVGRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPTPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAAATTTTTTESPPTTTTGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQTDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVRKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017598638.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurofascin (Nfasc), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598638.1

ORF Insert Sequence:

ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA 
TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA 
GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG 
AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG 
GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC 
CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT 
GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC 
CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG 
ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG 
TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG 
TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG 
GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT 
GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG 
GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA 
CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC 
AACAACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATATATAG TGCCCGAGGA 
GTTGCGGAAA GAACACCTAG CTTCATGTAT CCCCAGGGCA CGTCAAGCAG TCAGATGGTA 
CTGCGCGGCA TGGACCTGCT GCTGGAGTGC ATTGCCTCTG GGGTCCCAAC ACCAGATATT 
GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTCGA GAATTTTAAC 
AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCCCCATAC 
TGGCTGGATG AGCCCAAGAA CTTGATCCTG GCTCCTGGTG AAGATGGGAG GCTGGTGTGT 
CGAGCCAATG GGAACCCGAA GCCGACCGTC CAGTGGCTGG TGAATGGAGA CCCTTTGCAA 
TCGGCACCAC CCAACCCCAA CCGTGAGGTG GCCGGAGACA CTATCATCTT CCGGGACACT 
CAGATCAGCA GCAGGGCAGT GTACCAGTGT AACACATCCA ACGAACATGG CTACCTGCTG 
GCCAATGCCT TCGTCAGCGT ATTAGATGTA CCCCCTCGGA TGCTGTCTCC CCGGAACCAG 
CTCATCAGGG TGATCCTTTA CAACCGGACG CGACTGGACT GTCCGTTCTT TGGGTCTCCC 
ATTCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG TGGTAACTAC 
CACGTCTACG AAAATGGCAG CCTGGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC 
TACACCTGTG TGGCCACCAA CATCCTGGGA AAAGCTGAAA ATCAAGTCCG CCTGGAGGTC 
AAAGACCCCA CCAGGATCTA CAGGATGCCT GAAGACCAGG TGGCCAAGAG GGGCACCACA 
GTGCAGCTGG AGTGCCGTGT GAAGCATGAC CCCTCCTTGA AACTCACAGT CTCCTGGCTG 
AAGGATGACG AGCCACTCTA CATTGGAAAC AGGATGAAAA AGGAAGATGA CTCCCTGACC 
ATCTTCGGAG TGGCAGAGCG GGACCAAGGC AGTTACACGT GCATGGCCAG CACCGAGCTG 
GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCGACTAAC 
CGTTTGGCTG CCCTGCCCAA AGGGCGGCCA GACCGACCCA GGGACCTGGA GCTCACTGAC 
CTGGCGGAAA GGAGTGTGAG GCTGACCTGG ATCCCGGGGG ATGATAACAA CAGTCCCATC 
ACAGACTACG TTGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGAGTCTG GCATGACCAC 
TCCAAGTTCC CAGGCAGTGT CAACTCAGCC GTCCTCCATC TGTCCCCGTA TGTCAACTAT 
CAGTTCCGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG 
CGGTACCGAA CAAGCGGGGC ACCCCCTGAA TCCAACCCCA GTGATGTGAA GGGCGAAGGG 
ACACGAAAAA ACAATATGGA GATCACATGG ACGCCTATGA ATGCTACCTC CGCCTTTGGC 
CCCAACCTGC GCTACATTGT CAAGTGGCGA CGGAGAGAAA CCCGAGAGAC TTGGAACAAT 
GTCACCGTGT GGGGCTCTCG CTATGTGGTG GGGCAGACCC CTGTCTACGT ACCCTATGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCGG AGCCTGAAAC CGTCATTGGG 
TACTCGGGGG AAGATTATCC CAGGGCTGCA CCCACTGAAG TTAAAATCCG AGTCCTGAAC 
AGCACAGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCTG ACACGGTCCA GGGCCAGCTC 
AGAGAGTATC GAGCTTACTA CTGGAGGGAA AGCAGTTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGAC AGCAGGCCAG CTTCCCTGGC GACCGCCCCC GGGGCGTGGT GGGTCGCCTG 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG TGATGGGCCT 
CGAAGTGAAA CCAAGGAATT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
AGAGTCCGAC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGATCACCC AGAGCACCCC 
AACGGGATCC TGATTGGATA CACGCTCAGA TACGTGCCCT TTAATGGAAC CAAACTGGGA 
AAGCAGATGG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCTCGGTGCA GAGAGCAGAC 
CCCGTGTCCC GTTACCGCTT CTCCCTCAGT GCCAGGACGC AGGTGGGCTC TGGAGAAGCA 
GCCACGGAGG AGTCCCCAAC ACCTCCAAAT GAAGCTACTC CAACTGCAGC TCCTCCCACA 
TTGCCCCCGA CTACCGTGGG TACCACAGGC CTTGTGAGCA GTACTGATGC TACTGCCCTT 
GCTGCCACCA GTGAAGCCAC AACAGTTCCC ATCATCCCAA CCGTCGTACC TACCACCGTC 
GCCACCACCA TTGCCACAAC TACTACAACC ACTGCCGCCG CCACCACCAC TACCACCACG 
GAGAGTCCTC CCACTACCAC CACTGGGACT AAGATTCACG AAACCGCCCC CGACGAGCAG 
TCCATTTGGA ACGTCACGGT GCTCCCCAAC AGTAAATGGG CCAACATCAC CTGGAAGCAC 
AATTTCAGGC CTGGAACTGA CTTTGTGGTT GAGTACATCG ACAGCAACCA TACGAAAAAA 
ACTGTCCCTG TTAAGGCCCA AGCCCAGCCC ATACAGCTGA CAGACCTCTT TCCCGGGATG 
ACGTACACGT TGCGGGTGTA TTCCCGGGAC AACGAGGGCA TCAGCAGTAC CGTCATCACC 
TTTATGACCA GTACAGCTTA CACCAACAAC CAGACTGACA TCGCCACCCA GGGCTGGTTC 
ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG GTGCTGATCC TGCTTATCGT CTGCTTCATC 
AAGAGGAGTC GTGGCGGCAA GTACCCAGTG CGAGAAAAGA AGGATGTCCC CTTGGGCCCT 
GAAGACCCCA AAGAAGAAGA TGGTTCATTT GACTACAGTG ACGAGGACAA CAAGCCCCTG 
CAGGGCAGCC AGACATCTCT GGACGGCACC ATCAAGCAGC AGGAGAGTGA CGACAGCCTA 
GTGGACTACG GCGAGGGTGG CGAGGGCCAG TTCAACGAAG ATGGCTCCTT TATTGGCCAG 
TACACTGTCA GAAAGGACAA GGAGGAGACC GAGGGCAATG AGAGCTCAGA GGCCACATCT 
CCAGTCAATG CCATCTATTC CCTGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_006249727.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249789.1 Rattus norvegicus neurofascin (Nfasc), transcript variant X2, mRNA.

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CloneID	ORa35271
Clone ID Related Accession (Same CDS sequence)	XM_017598638.1 , XM_006249727.3
Accession Version	XM_006249727.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neurofascin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249727.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA 
TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA 
GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG 
AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG 
GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC 
CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT 
GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC 
CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG 
ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG 
TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG 
TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG 
GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT 
GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG 
GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA 
CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC 
AACAACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATATATAG TGCCCGAGGA 
GTTGCGGAAA GAACACCTAG CTTCATGTAT CCCCAGGGCA CGTCAAGCAG TCAGATGGTA 
CTGCGCGGCA TGGACCTGCT GCTGGAGTGC ATTGCCTCTG GGGTCCCAAC ACCAGATATT 
GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTCGA GAATTTTAAC 
AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCCCCATAC 
TGGCTGGATG AGCCCAAGAA CTTGATCCTG GCTCCTGGTG AAGATGGGAG GCTGGTGTGT 
CGAGCCAATG GGAACCCGAA GCCGACCGTC CAGTGGCTGG TGAATGGAGA CCCTTTGCAA 
TCGGCACCAC CCAACCCCAA CCGTGAGGTG GCCGGAGACA CTATCATCTT CCGGGACACT 
CAGATCAGCA GCAGGGCAGT GTACCAGTGT AACACATCCA ACGAACATGG CTACCTGCTG 
GCCAATGCCT TCGTCAGCGT ATTAGATGTA CCCCCTCGGA TGCTGTCTCC CCGGAACCAG 
CTCATCAGGG TGATCCTTTA CAACCGGACG CGACTGGACT GTCCGTTCTT TGGGTCTCCC 
ATTCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG TGGTAACTAC 
CACGTCTACG AAAATGGCAG CCTGGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC 
TACACCTGTG TGGCCACCAA CATCCTGGGA AAAGCTGAAA ATCAAGTCCG CCTGGAGGTC 
AAAGACCCCA CCAGGATCTA CAGGATGCCT GAAGACCAGG TGGCCAAGAG GGGCACCACA 
GTGCAGCTGG AGTGCCGTGT GAAGCATGAC CCCTCCTTGA AACTCACAGT CTCCTGGCTG 
AAGGATGACG AGCCACTCTA CATTGGAAAC AGGATGAAAA AGGAAGATGA CTCCCTGACC 
ATCTTCGGAG TGGCAGAGCG GGACCAAGGC AGTTACACGT GCATGGCCAG CACCGAGCTG 
GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCGACTAAC 
CGTTTGGCTG CCCTGCCCAA AGGGCGGCCA GACCGACCCA GGGACCTGGA GCTCACTGAC 
CTGGCGGAAA GGAGTGTGAG GCTGACCTGG ATCCCGGGGG ATGATAACAA CAGTCCCATC 
ACAGACTACG TTGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGAGTCTG GCATGACCAC 
TCCAAGTTCC CAGGCAGTGT CAACTCAGCC GTCCTCCATC TGTCCCCGTA TGTCAACTAT 
CAGTTCCGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG 
CGGTACCGAA CAAGCGGGGC ACCCCCTGAA TCCAACCCCA GTGATGTGAA GGGCGAAGGG 
ACACGAAAAA ACAATATGGA GATCACATGG ACGCCTATGA ATGCTACCTC CGCCTTTGGC 
CCCAACCTGC GCTACATTGT CAAGTGGCGA CGGAGAGAAA CCCGAGAGAC TTGGAACAAT 
GTCACCGTGT GGGGCTCTCG CTATGTGGTG GGGCAGACCC CTGTCTACGT ACCCTATGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCGG AGCCTGAAAC CGTCATTGGG 
TACTCGGGGG AAGATTATCC CAGGGCTGCA CCCACTGAAG TTAAAATCCG AGTCCTGAAC 
AGCACAGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCTG ACACGGTCCA GGGCCAGCTC 
AGAGAGTATC GAGCTTACTA CTGGAGGGAA AGCAGTTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGAC AGCAGGCCAG CTTCCCTGGC GACCGCCCCC GGGGCGTGGT GGGTCGCCTG 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG TGATGGGCCT 
CGAAGTGAAA CCAAGGAATT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
AGAGTCCGAC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGATCACCC AGAGCACCCC 
AACGGGATCC TGATTGGATA CACGCTCAGA TACGTGCCCT TTAATGGAAC CAAACTGGGA 
AAGCAGATGG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCTCGGTGCA GAGAGCAGAC 
CCCGTGTCCC GTTACCGCTT CTCCCTCAGT GCCAGGACGC AGGTGGGCTC TGGAGAAGCA 
GCCACGGAGG AGTCCCCAAC ACCTCCAAAT GAAGCTACTC CAACTGCAGC TCCTCCCACA 
TTGCCCCCGA CTACCGTGGG TACCACAGGC CTTGTGAGCA GTACTGATGC TACTGCCCTT 
GCTGCCACCA GTGAAGCCAC AACAGTTCCC ATCATCCCAA CCGTCGTACC TACCACCGTC 
GCCACCACCA TTGCCACAAC TACTACAACC ACTGCCGCCG CCACCACCAC TACCACCACG 
GAGAGTCCTC CCACTACCAC CACTGGGACT AAGATTCACG AAACCGCCCC CGACGAGCAG 
TCCATTTGGA ACGTCACGGT GCTCCCCAAC AGTAAATGGG CCAACATCAC CTGGAAGCAC 
AATTTCAGGC CTGGAACTGA CTTTGTGGTT GAGTACATCG ACAGCAACCA TACGAAAAAA 
ACTGTCCCTG TTAAGGCCCA AGCCCAGCCC ATACAGCTGA CAGACCTCTT TCCCGGGATG 
ACGTACACGT TGCGGGTGTA TTCCCGGGAC AACGAGGGCA TCAGCAGTAC CGTCATCACC 
TTTATGACCA GTACAGCTTA CACCAACAAC CAGACTGACA TCGCCACCCA GGGCTGGTTC 
ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG GTGCTGATCC TGCTTATCGT CTGCTTCATC 
AAGAGGAGTC GTGGCGGCAA GTACCCAGTG CGAGAAAAGA AGGATGTCCC CTTGGGCCCT 
GAAGACCCCA AAGAAGAAGA TGGTTCATTT GACTACAGTG ACGAGGACAA CAAGCCCCTG 
CAGGGCAGCC AGACATCTCT GGACGGCACC ATCAAGCAGC AGGAGAGTGA CGACAGCCTA 
GTGGACTACG GCGAGGGTGG CGAGGGCCAG TTCAACGAAG ATGGCTCCTT TATTGGCCAG 
TACACTGTCA GAAAGGACAA GGAGGAGACC GAGGGCAATG AGAGCTCAGA GGCCACATCT 
CCAGTCAATG CCATCTATTC CCTGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249789.1
CDS	53..4462
Translation	MRRLAARAAAGDAGEVAAPGADIWDCAQGWSWSSWRLTDLCAIWEPGVFLPSAAAVNRSRASAGVAKSRPNEAEKLSTVWKAHEERRKASRARGLGSAEQGLALRMARQQAPPWVHVALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWLVNGDPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPETVIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVGRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPTPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAAATTTTTTESPPTTTTGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQTDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVRKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_006249727.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurofascin (Nfasc), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249727.3

ORF Insert Sequence:

ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA 
TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA 
GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG 
AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG 
GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC 
CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT 
GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC 
CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG 
ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG 
TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG 
TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG 
GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT 
GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG 
GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA 
CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC 
AACAACCCCT ATAATGACTC GTCCTTAAGA AACCACCCTG ACATATATAG TGCCCGAGGA 
GTTGCGGAAA GAACACCTAG CTTCATGTAT CCCCAGGGCA CGTCAAGCAG TCAGATGGTA 
CTGCGCGGCA TGGACCTGCT GCTGGAGTGC ATTGCCTCTG GGGTCCCAAC ACCAGATATT 
GCATGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTCGA GAATTTTAAC 
AAGGCTCTGC GCATCACCAA TGTCTCTGAA GAGGACTCTG GGGAGTATTT CTGCCTGGCC 
TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCCCCATAC 
TGGCTGGATG AGCCCAAGAA CTTGATCCTG GCTCCTGGTG AAGATGGGAG GCTGGTGTGT 
CGAGCCAATG GGAACCCGAA GCCGACCGTC CAGTGGCTGG TGAATGGAGA CCCTTTGCAA 
TCGGCACCAC CCAACCCCAA CCGTGAGGTG GCCGGAGACA CTATCATCTT CCGGGACACT 
CAGATCAGCA GCAGGGCAGT GTACCAGTGT AACACATCCA ACGAACATGG CTACCTGCTG 
GCCAATGCCT TCGTCAGCGT ATTAGATGTA CCCCCTCGGA TGCTGTCTCC CCGGAACCAG 
CTCATCAGGG TGATCCTTTA CAACCGGACG CGACTGGACT GTCCGTTCTT TGGGTCTCCC 
ATTCCAACAC TCCGATGGTT TAAGAATGGG CAAGGAAGCA ACCTGGATGG TGGTAACTAC 
CACGTCTACG AAAATGGCAG CCTGGAAATC AAGATGATTC GCAAAGAGGA CCAAGGCATC 
TACACCTGTG TGGCCACCAA CATCCTGGGA AAAGCTGAAA ATCAAGTCCG CCTGGAGGTC 
AAAGACCCCA CCAGGATCTA CAGGATGCCT GAAGACCAGG TGGCCAAGAG GGGCACCACA 
GTGCAGCTGG AGTGCCGTGT GAAGCATGAC CCCTCCTTGA AACTCACAGT CTCCTGGCTG 
AAGGATGACG AGCCACTCTA CATTGGAAAC AGGATGAAAA AGGAAGATGA CTCCCTGACC 
ATCTTCGGAG TGGCAGAGCG GGACCAAGGC AGTTACACGT GCATGGCCAG CACCGAGCTG 
GACCAGGACC TGGCAAAGGC CTACCTCACT GTTCTAGCTG ATCAGGCCAC TCCGACTAAC 
CGTTTGGCTG CCCTGCCCAA AGGGCGGCCA GACCGACCCA GGGACCTGGA GCTCACTGAC 
CTGGCGGAAA GGAGTGTGAG GCTGACCTGG ATCCCGGGGG ATGATAACAA CAGTCCCATC 
ACAGACTACG TTGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGAGTCTG GCATGACCAC 
TCCAAGTTCC CAGGCAGTGT CAACTCAGCC GTCCTCCATC TGTCCCCGTA TGTCAACTAT 
CAGTTCCGAG TCATCGCTGT CAACGAGGTT GGGAGCAGCC ACCCCAGCCT TCCATCCGAG 
CGGTACCGAA CAAGCGGGGC ACCCCCTGAA TCCAACCCCA GTGATGTGAA GGGCGAAGGG 
ACACGAAAAA ACAATATGGA GATCACATGG ACGCCTATGA ATGCTACCTC CGCCTTTGGC 
CCCAACCTGC GCTACATTGT CAAGTGGCGA CGGAGAGAAA CCCGAGAGAC TTGGAACAAT 
GTCACCGTGT GGGGCTCTCG CTATGTGGTG GGGCAGACCC CTGTCTACGT ACCCTATGAG 
ATCCGAGTCC AGGCTGAAAA TGACTTTGGG AAAGGCCCGG AGCCTGAAAC CGTCATTGGG 
TACTCGGGGG AAGATTATCC CAGGGCTGCA CCCACTGAAG TTAAAATCCG AGTCCTGAAC 
AGCACAGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCTG ACACGGTCCA GGGCCAGCTC 
AGAGAGTATC GAGCTTACTA CTGGAGGGAA AGCAGTTTGC TGAAGAACCT GTGGGTGTCT 
CAGAAGAGAC AGCAGGCCAG CTTCCCTGGC GACCGCCCCC GGGGCGTGGT GGGTCGCCTG 
TTCCCCTACA GTAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG TGATGGGCCT 
CGAAGTGAAA CCAAGGAATT CACCACCCCG GAAGGAGTAC CCAGTGCCCC CAGGCGTTTC 
AGAGTCCGAC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGATCACCC AGAGCACCCC 
AACGGGATCC TGATTGGATA CACGCTCAGA TACGTGCCCT TTAATGGAAC CAAACTGGGA 
AAGCAGATGG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCTCGGTGCA GAGAGCAGAC 
CCCGTGTCCC GTTACCGCTT CTCCCTCAGT GCCAGGACGC AGGTGGGCTC TGGAGAAGCA 
GCCACGGAGG AGTCCCCAAC ACCTCCAAAT GAAGCTACTC CAACTGCAGC TCCTCCCACA 
TTGCCCCCGA CTACCGTGGG TACCACAGGC CTTGTGAGCA GTACTGATGC TACTGCCCTT 
GCTGCCACCA GTGAAGCCAC AACAGTTCCC ATCATCCCAA CCGTCGTACC TACCACCGTC 
GCCACCACCA TTGCCACAAC TACTACAACC ACTGCCGCCG CCACCACCAC TACCACCACG 
GAGAGTCCTC CCACTACCAC CACTGGGACT AAGATTCACG AAACCGCCCC CGACGAGCAG 
TCCATTTGGA ACGTCACGGT GCTCCCCAAC AGTAAATGGG CCAACATCAC CTGGAAGCAC 
AATTTCAGGC CTGGAACTGA CTTTGTGGTT GAGTACATCG ACAGCAACCA TACGAAAAAA 
ACTGTCCCTG TTAAGGCCCA AGCCCAGCCC ATACAGCTGA CAGACCTCTT TCCCGGGATG 
ACGTACACGT TGCGGGTGTA TTCCCGGGAC AACGAGGGCA TCAGCAGTAC CGTCATCACC 
TTTATGACCA GTACAGCTTA CACCAACAAC CAGACTGACA TCGCCACCCA GGGCTGGTTC 
ATCGGGCTCA TGTGTGCCAT TGCCCTTCTG GTGCTGATCC TGCTTATCGT CTGCTTCATC 
AAGAGGAGTC GTGGCGGCAA GTACCCAGTG CGAGAAAAGA AGGATGTCCC CTTGGGCCCT 
GAAGACCCCA AAGAAGAAGA TGGTTCATTT GACTACAGTG ACGAGGACAA CAAGCCCCTG 
CAGGGCAGCC AGACATCTCT GGACGGCACC ATCAAGCAGC AGGAGAGTGA CGACAGCCTA 
GTGGACTACG GCGAGGGTGG CGAGGGCCAG TTCAACGAAG ATGGCTCCTT TATTGGCCAG 
TACACTGTCA GAAAGGACAA GGAGGAGACC GAGGGCAATG AGAGCTCAGA GGCCACATCT 
CCAGTCAATG CCATCTATTC CCTGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_006249734.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249796.1 Rattus norvegicus neurofascin (Nfasc), transcript variant X9, mRNA.

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CloneID	ORa35278
Clone ID Related Accession (Same CDS sequence)	XM_006249734.3
Accession Version	XM_006249734.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neurofascin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249734.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA 
TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA 
GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG 
AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG 
GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC 
CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT 
GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC 
CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG 
ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG 
TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG 
TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG 
GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT 
GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG 
GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA 
CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC 
ACCCGAGGAG TTGCGGAAAG AACACCTAGC TTCATGTATC CCCAGGGCAC GTCAAGCAGT 
CAGATGGTAC TGCGCGGCAT GGACCTGCTG CTGGAGTGCA TTGCCTCTGG GGTCCCAACA 
CCAGATATTG CATGGTACAA GAAAGGTGGG GACCTCCCAT CTGACAAGGC CAAGTTCGAG 
AATTTTAACA AGGCTCTGCG CATCACCAAT GTCTCTGAAG AGGACTCTGG GGAGTATTTC 
TGCCTGGCCT CCAACAAGAT GGGCAGCATC CGGCACACGA TCTCGGTGAG AGTAAAGGCT 
GCCCCATACT GGCTGGATGA GCCCAAGAAC TTGATCCTGG CTCCTGGTGA AGATGGGAGG 
CTGGTGTGTC GAGCCAATGG GAACCCGAAG CCGACCGTCC AGTGGCTGGT GAATGGAGAC 
CCTTTGCAAT CGGCACCACC CAACCCCAAC CGTGAGGTGG CCGGAGACAC TATCATCTTC 
CGGGACACTC AGATCAGCAG CAGGGCAGTG TACCAGTGTA ACACATCCAA CGAACATGGC 
TACCTGCTGG CCAATGCCTT CGTCAGCGTA TTAGATGTAC CCCCTCGGAT GCTGTCTCCC 
CGGAACCAGC TCATCAGGGT GATCCTTTAC AACCGGACGC GACTGGACTG TCCGTTCTTT 
GGGTCTCCCA TTCCAACACT CCGATGGTTT AAGAATGGGC AAGGAAGCAA CCTGGATGGT 
GGTAACTACC ACGTCTACGA AAATGGCAGC CTGGAAATCA AGATGATTCG CAAAGAGGAC 
CAAGGCATCT ACACCTGTGT GGCCACCAAC ATCCTGGGAA AAGCTGAAAA TCAAGTCCGC 
CTGGAGGTCA AAGACCCCAC CAGGATCTAC AGGATGCCTG AAGACCAGGT GGCCAAGAGG 
GGCACCACAG TGCAGCTGGA GTGCCGTGTG AAGCATGACC CCTCCTTGAA ACTCACAGTC 
TCCTGGCTGA AGGATGACGA GCCACTCTAC ATTGGAAACA GGATGAAAAA GGAAGATGAC 
TCCCTGACCA TCTTCGGAGT GGCAGAGCGG GACCAAGGCA GTTACACGTG CATGGCCAGC 
ACCGAGCTGG ACCAGGACCT GGCAAAGGCC TACCTCACTG TTCTAGCTGA TCAGGCCACT 
CCGACTAACC GTTTGGCTGC CCTGCCCAAA GGGCGGCCAG ACCGACCCAG GGACCTGGAG 
CTCACTGACC TGGCGGAAAG GAGTGTGAGG CTGACCTGGA TCCCGGGGGA TGATAACAAC 
AGTCCCATCA CAGACTACGT TGTCCAGTTT GAAGAGGACC AGTTCCAGCC AGGAGTCTGG 
CATGACCACT CCAAGTTCCC AGGCAGTGTC AACTCAGCCG TCCTCCATCT GTCCCCGTAT 
GTCAACTATC AGTTCCGAGT CATCGCTGTC AACGAGGTTG GGAGCAGCCA CCCCAGCCTT 
CCATCCGAGC GGTACCGAAC AAGCGGGGCA CCCCCTGAAT CCAACCCCAG TGATGTGAAG 
GGCGAAGGGA CACGAAAAAA CAATATGGAG ATCACATGGA CGCCTATGAA TGCTACCTCC 
GCCTTTGGCC CCAACCTGCG CTACATTGTC AAGTGGCGAC GGAGAGAAAC CCGAGAGACT 
TGGAACAATG TCACCGTGTG GGGCTCTCGC TATGTGGTGG GGCAGACCCC TGTCTACGTA 
CCCTATGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AAGGCCCGGA GCCTGAAACC 
GTCATTGGGT ACTCGGGGGA AGATTTACCC AGTGCCCCCA GGCGTTTCAG AGTCCGACAG 
CCCAACCTGG AGACCATCAA CCTGGAATGG GATCACCCAG AGCACCCCAA CGGGATCCTG 
ATTGGATACA CGCTCAGATA CGTGCCCTTT AATGGAACCA AACTGGGAAA GCAGATGGTG 
GAAAACTTCT CTCCCAATCA GACCAAGTTC TCGGTGCAGA GAGCAGACCC CGTGTCCCGT 
TACCGCTTCT CCCTCAGTGC CAGGACGCAG GTGGGCTCTG GAGAAGCAGC CACGGAGGAG 
TCCCCAACAC CTCCAAATGA AGCTACTCCA ACTGCAGCTC CTCCCACATT GCCCCCGACT 
ACCGTGGGTA CCACAGGCCT TGTGAGCAGT ACTGATGCTA CTGCCCTTGC TGCCACCAGT 
GAAGCCACAA CAGTTCCCAT CATCCCAACC GTCGTACCTA CCACCGTCGC CACCACCATT 
GCCACAACTA CTACAACCAC TGCCGCCGCC ACCACCACTA CCACCACGGA GAGTCCTCCC 
ACTACCACCA CTGGGACTAA GATTCACGAA ACCGCCCCCG ACGAGCAGTC CATTTGGAAC 
GTCACGGTGC TCCCCAACAG TAAATGGGCC AACATCACCT GGAAGCACAA TTTCAGGCCT 
GGAACTGACT TTGTGGTTGA GTACATCGAC AGCAACCATA CGAAAAAAAC TGTCCCTGTT 
AAGGCCCAAG CCCAGCCCAT ACAGCTGACA GACCTCTTTC CCGGGATGAC GTACACGTTG 
CGGGTGTATT CCCGGGACAA CGAGGGCATC AGCAGTACCG TCATCACCTT TATGACCAGT 
ACAGCTTACA CCAACAACCA GACTGACATC GCCACCCAGG GCTGGTTCAT CGGGCTCATG 
TGTGCCATTG CCCTTCTGGT GCTGATCCTG CTTATCGTCT GCTTCATCAA GAGGAGTCGT 
GGCGGCAAGT ACCCAGTGCG AGAAAAGAAG GATGTCCCCT TGGGCCCTGA AGACCCCAAA 
GAAGAAGATG GTTCATTTGA CTACAGTGAC GAGGACAACA AGCCCCTGCA GGGCAGCCAG 
ACATCTCTGG ACGGCACCAT CAAGCAGCAG GAGAGTGACG ACAGCCTAGT GGACTACGGC 
GAGGGTGGCG AGGGCCAGTT CAACGAAGAT GGCTCCTTTA TTGGCCAGTA CACTGTCAGA 
AAGGACAAGG AGGAGACCGA GGGCAATGAG AGCTCAGAGG CCACATCTCC AGTCAATGCC 
ATCTATTCCC TGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249796.1
CDS	49..4086
Translation	MRRLAARAAAGDAGEVAAPGADIWDCAQGWSWSSWRLTDLCAIWEPGVFLPSAAAVNRSRASAGVAKSRPNEAEKLSTVWKAHEERRKASRARGLGSAEQGLALRMARQQAPPWVHVALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWLVNGDPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPETVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPTPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAAATTTTTTESPPTTTTGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQTDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVRKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_006249734.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurofascin (Nfasc), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249734.3

ORF Insert Sequence:

ATGCGGCGGC TGGCGGCGAG AGCTGCTGCA GGGGACGCGG GCGAAGTGGC GGCGCCGGGA 
GCAGACATCT GGGACTGCGC CCAGGGCTGG AGCTGGAGCT CCTGGAGGTT GACTGACTTA 
TGTGCGATTT GGGAGCCTGG AGTTTTCTTG CCCTCTGCCG CTGCCGTCAA CCGGAGCAGA 
GCCTCCGCTG GTGTTGCGAA AAGCAGGCCG AATGAGGCAG AGAAGCTGAG CACTGTCTGG 
AAAGCCCACG AGGAACGGCG CAAGGCATCG AGAGCCCGTG GGCTGGGCAG TGCGGAGCAG 
GGCCTGGCGC TGAGGATGGC CAGGCAGCAG GCGCCACCCT GGGTCCACGT AGCCCTCATC 
CTCTTCCTCC TCAGCCTCGG AGGGGCCATT GAGATTCCGA TGGATCCAAG CATTCAGAAT 
GAGCTGACCC AACCCCCAAC GATCACCAAG CAGTCGGTGA AGGACCACAT CGTGGACCCC 
CGAGATAACA TCCTGATTGA ATGTGAAGCT AAAGGGAACC CCGCCCCCAG TTTCCACTGG 
ACTCGCAACA GCAGGTTCTT CAACATTGCC AAGGACCCAC GGGTGTCCAT GAGGAGGAGG 
TCTGGGACCT TGGTGATCGA CTTCCGCAGT GGTGGGCGGC CTGAGGAGTA CGAAGGGGAG 
TACCAGTGCT TTGCCCGGAA CAAATTCGGC ACAGCTCTTA GCAACCGCAT CCGCCTGCAG 
GTGTCCAAAT CTCCCCTGTG GCCCAAGGAA AACCTAGACC CCGTCGTGGT TCAAGAGGGT 
GCCCCCTTAA CCCTGCAGTG CAACCCCCCA CCTGGCCTCC CATCCCCCGT CATCTTCTGG 
ATGAGCAGCT CCATGGAGCC CATCACCCAG GACAAGCGTG TCTCCCAGGG TCACAACGGG 
GACCTGTACT TCTCCAACGT CATGCTGCAG GACATGCAGA CCGACTACAG CTGCAATGCA 
CGCTTCCACT TCACCCACAC CATTCAGCAG AAGAATCCCT TCACCCTCAA AGTCCTCACC 
ACCCGAGGAG TTGCGGAAAG AACACCTAGC TTCATGTATC CCCAGGGCAC GTCAAGCAGT 
CAGATGGTAC TGCGCGGCAT GGACCTGCTG CTGGAGTGCA TTGCCTCTGG GGTCCCAACA 
CCAGATATTG CATGGTACAA GAAAGGTGGG GACCTCCCAT CTGACAAGGC CAAGTTCGAG 
AATTTTAACA AGGCTCTGCG CATCACCAAT GTCTCTGAAG AGGACTCTGG GGAGTATTTC 
TGCCTGGCCT CCAACAAGAT GGGCAGCATC CGGCACACGA TCTCGGTGAG AGTAAAGGCT 
GCCCCATACT GGCTGGATGA GCCCAAGAAC TTGATCCTGG CTCCTGGTGA AGATGGGAGG 
CTGGTGTGTC GAGCCAATGG GAACCCGAAG CCGACCGTCC AGTGGCTGGT GAATGGAGAC 
CCTTTGCAAT CGGCACCACC CAACCCCAAC CGTGAGGTGG CCGGAGACAC TATCATCTTC 
CGGGACACTC AGATCAGCAG CAGGGCAGTG TACCAGTGTA ACACATCCAA CGAACATGGC 
TACCTGCTGG CCAATGCCTT CGTCAGCGTA TTAGATGTAC CCCCTCGGAT GCTGTCTCCC 
CGGAACCAGC TCATCAGGGT GATCCTTTAC AACCGGACGC GACTGGACTG TCCGTTCTTT 
GGGTCTCCCA TTCCAACACT CCGATGGTTT AAGAATGGGC AAGGAAGCAA CCTGGATGGT 
GGTAACTACC ACGTCTACGA AAATGGCAGC CTGGAAATCA AGATGATTCG CAAAGAGGAC 
CAAGGCATCT ACACCTGTGT GGCCACCAAC ATCCTGGGAA AAGCTGAAAA TCAAGTCCGC 
CTGGAGGTCA AAGACCCCAC CAGGATCTAC AGGATGCCTG AAGACCAGGT GGCCAAGAGG 
GGCACCACAG TGCAGCTGGA GTGCCGTGTG AAGCATGACC CCTCCTTGAA ACTCACAGTC 
TCCTGGCTGA AGGATGACGA GCCACTCTAC ATTGGAAACA GGATGAAAAA GGAAGATGAC 
TCCCTGACCA TCTTCGGAGT GGCAGAGCGG GACCAAGGCA GTTACACGTG CATGGCCAGC 
ACCGAGCTGG ACCAGGACCT GGCAAAGGCC TACCTCACTG TTCTAGCTGA TCAGGCCACT 
CCGACTAACC GTTTGGCTGC CCTGCCCAAA GGGCGGCCAG ACCGACCCAG GGACCTGGAG 
CTCACTGACC TGGCGGAAAG GAGTGTGAGG CTGACCTGGA TCCCGGGGGA TGATAACAAC 
AGTCCCATCA CAGACTACGT TGTCCAGTTT GAAGAGGACC AGTTCCAGCC AGGAGTCTGG 
CATGACCACT CCAAGTTCCC AGGCAGTGTC AACTCAGCCG TCCTCCATCT GTCCCCGTAT 
GTCAACTATC AGTTCCGAGT CATCGCTGTC AACGAGGTTG GGAGCAGCCA CCCCAGCCTT 
CCATCCGAGC GGTACCGAAC AAGCGGGGCA CCCCCTGAAT CCAACCCCAG TGATGTGAAG 
GGCGAAGGGA CACGAAAAAA CAATATGGAG ATCACATGGA CGCCTATGAA TGCTACCTCC 
GCCTTTGGCC CCAACCTGCG CTACATTGTC AAGTGGCGAC GGAGAGAAAC CCGAGAGACT 
TGGAACAATG TCACCGTGTG GGGCTCTCGC TATGTGGTGG GGCAGACCCC TGTCTACGTA 
CCCTATGAGA TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AAGGCCCGGA GCCTGAAACC 
GTCATTGGGT ACTCGGGGGA AGATTTACCC AGTGCCCCCA GGCGTTTCAG AGTCCGACAG 
CCCAACCTGG AGACCATCAA CCTGGAATGG GATCACCCAG AGCACCCCAA CGGGATCCTG 
ATTGGATACA CGCTCAGATA CGTGCCCTTT AATGGAACCA AACTGGGAAA GCAGATGGTG 
GAAAACTTCT CTCCCAATCA GACCAAGTTC TCGGTGCAGA GAGCAGACCC CGTGTCCCGT 
TACCGCTTCT CCCTCAGTGC CAGGACGCAG GTGGGCTCTG GAGAAGCAGC CACGGAGGAG 
TCCCCAACAC CTCCAAATGA AGCTACTCCA ACTGCAGCTC CTCCCACATT GCCCCCGACT 
ACCGTGGGTA CCACAGGCCT TGTGAGCAGT ACTGATGCTA CTGCCCTTGC TGCCACCAGT 
GAAGCCACAA CAGTTCCCAT CATCCCAACC GTCGTACCTA CCACCGTCGC CACCACCATT 
GCCACAACTA CTACAACCAC TGCCGCCGCC ACCACCACTA CCACCACGGA GAGTCCTCCC 
ACTACCACCA CTGGGACTAA GATTCACGAA ACCGCCCCCG ACGAGCAGTC CATTTGGAAC 
GTCACGGTGC TCCCCAACAG TAAATGGGCC AACATCACCT GGAAGCACAA TTTCAGGCCT 
GGAACTGACT TTGTGGTTGA GTACATCGAC AGCAACCATA CGAAAAAAAC TGTCCCTGTT 
AAGGCCCAAG CCCAGCCCAT ACAGCTGACA GACCTCTTTC CCGGGATGAC GTACACGTTG 
CGGGTGTATT CCCGGGACAA CGAGGGCATC AGCAGTACCG TCATCACCTT TATGACCAGT 
ACAGCTTACA CCAACAACCA GACTGACATC GCCACCCAGG GCTGGTTCAT CGGGCTCATG 
TGTGCCATTG CCCTTCTGGT GCTGATCCTG CTTATCGTCT GCTTCATCAA GAGGAGTCGT 
GGCGGCAAGT ACCCAGTGCG AGAAAAGAAG GATGTCCCCT TGGGCCCTGA AGACCCCAAA 
GAAGAAGATG GTTCATTTGA CTACAGTGAC GAGGACAACA AGCCCCTGCA GGGCAGCCAG 
ACATCTCTGG ACGGCACCAT CAAGCAGCAG GAGAGTGACG ACAGCCTAGT GGACTACGGC 
GAGGGTGGCG AGGGCCAGTT CAACGAAGAT GGCTCCTTTA TTGGCCAGTA CACTGTCAGA 
AAGGACAAGG AGGAGACCGA GGGCAATGAG AGCTCAGAGG CCACATCTCC AGTCAATGCC 
ATCTATTCCC TGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_017598651.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454140.1 Rattus norvegicus neurofascin (Nfasc), transcript variant X17, mRNA.

Price & Availability↑

CloneID	ORa35285
Clone ID Related Accession (Same CDS sequence)	XM_017598651.1 , XM_017598652.1
Accession Version	XM_017598651.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neurofascin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACGTAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATTGAGAT TCCGATGGAT CCAAGCATTC AGAATGAGCT GACCCAACCC 
CCAACGATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ACCCCCGAGA TAACATCCTG 
ATTGAATGTG AAGCTAAAGG GAACCCCGCC CCCAGTTTCC ACTGGACTCG CAACAGCAGG 
TTCTTCAACA TTGCCAAGGA CCCACGGGTG TCCATGAGGA GGAGGTCTGG GACCTTGGTG 
ATCGACTTCC GCAGTGGTGG GCGGCCTGAG GAGTACGAAG GGGAGTACCA GTGCTTTGCC 
CGGAACAAAT TCGGCACAGC TCTTAGCAAC CGCATCCGCC TGCAGGTGTC CAAATCTCCC 
CTGTGGCCCA AGGAAAACCT AGACCCCGTC GTGGTTCAAG AGGGTGCCCC CTTAACCCTG 
CAGTGCAACC CCCCACCTGG CCTCCCATCC CCCGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAGGACAA GCGTGTCTCC CAGGGTCACA ACGGGGACCT GTACTTCTCC 
AACGTCATGC TGCAGGACAT GCAGACCGAC TACAGCTGCA ATGCACGCTT CCACTTCACC 
CACACCATTC AGCAGAAGAA TCCCTTCACC CTCAAAGTCC TCACCAACAA CCCCTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATA TATAGTGCCC GAGGAGTTGC GGAAAGAACA 
CCTAGCTTCA TGTATCCCCA GGGCACGTCA AGCAGTCAGA TGGTACTGCG CGGCATGGAC 
CTGCTGCTGG AGTGCATTGC CTCTGGGGTC CCAACACCAG ATATTGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTCGAGAATT TTAACAAGGC TCTGCGCATC 
ACCAATGTCT CTGAAGAGGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCCC CATACTGGCT GGATGAGCCC 
AAGAACTTGA TCCTGGCTCC TGGTGAAGAT GGGAGGCTGG TGTGTCGAGC CAATGGGAAC 
CCGAAGCCGA CCGTCCAGTG GCTGGTGAAT GGAGACCCTT TGCAATCGGC ACCACCCAAC 
CCCAACCGTG AGGTGGCCGG AGACACTATC ATCTTCCGGG ACACTCAGAT CAGCAGCAGG 
GCAGTGTACC AGTGTAACAC ATCCAACGAA CATGGCTACC TGCTGGCCAA TGCCTTCGTC 
AGCGTATTAG ATGTACCCCC TCGGATGCTG TCTCCCCGGA ACCAGCTCAT CAGGGTGATC 
CTTTACAACC GGACGCGACT GGACTGTCCG TTCTTTGGGT CTCCCATTCC AACACTCCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGTA ACTACCACGT CTACGAAAAT 
GGCAGCCTGG AAATCAAGAT GATTCGCAAA GAGGACCAAG GCATCTACAC CTGTGTGGCC 
ACCAACATCC TGGGAAAAGC TGAAAATCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACAGGA TGCCTGAAGA CCAGGTGGCC AAGAGGGGCA CCACAGTGCA GCTGGAGTGC 
CGTGTGAAGC ATGACCCCTC CTTGAAACTC ACAGTCTCCT GGCTGAAGGA TGACGAGCCA 
CTCTACATTG GAAACAGGAT GAAAAAGGAA GATGACTCCC TGACCATCTT CGGAGTGGCA 
GAGCGGGACC AAGGCAGTTA CACGTGCATG GCCAGCACCG AGCTGGACCA GGACCTGGCA 
AAGGCCTACC TCACTGTTCT AGCTGATCAG GCCACTCCGA CTAACCGTTT GGCTGCCCTG 
CCCAAAGGGC GGCCAGACCG ACCCAGGGAC CTGGAGCTCA CTGACCTGGC GGAAAGGAGT 
GTGAGGCTGA CCTGGATCCC GGGGGATGAT AACAACAGTC CCATCACAGA CTACGTTGTC 
CAGTTTGAAG AGGACCAGTT CCAGCCAGGA GTCTGGCATG ACCACTCCAA GTTCCCAGGC 
AGTGTCAACT CAGCCGTCCT CCATCTGTCC CCGTATGTCA ACTATCAGTT CCGAGTCATC 
GCTGTCAACG AGGTTGGGAG CAGCCACCCC AGCCTTCCAT CCGAGCGGTA CCGAACAAGC 
GGGGCACCCC CTGAATCCAA CCCCAGTGAT GTGAAGGGCG AAGGGACACG AAAAAACAAT 
ATGGAGATCA CATGGACGCC TATGAATGCT ACCTCCGCCT TTGGCCCCAA CCTGCGCTAC 
ATTGTCAAGT GGCGACGGAG AGAAACCCGA GAGACTTGGA ACAATGTCAC CGTGTGGGGC 
TCTCGCTATG TGGTGGGGCA GACCCCTGTC TACGTACCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TTGGGAAAGG CCCGGAGCCT GAAACCGTCA TTGGGTACTC GGGGGAAGAT 
TATCCCAGGG CTGCACCCAC TGAAGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGC 
CTTCAGTGGA ACCGCGTCTA CTCTGACACG GTCCAGGGCC AGCTCAGAGA GTATCGAGCT 
TACTACTGGA GGGAAAGCAG TTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAG 
GCCAGCTTCC CTGGCGACCG CCCCCGGGGC GTGGTGGGTC GCCTGTTCCC CTACAGTAAC 
TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGTGATG GGCCTCGAAG TGAAACCAAG 
GAATTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGGC GTTTCAGAGT CCGACAGCCC 
AACCTGGAGA CCATCAACCT GGAATGGGAT CACCCAGAGC ACCCCAACGG GATCCTGATT 
GGATACACGC TCAGATACGT GCCCTTTAAT GGAACCAAAC TGGGAAAGCA GATGGTGGAA 
AACTTCTCTC CCAATCAGAC CAAGTTCTCG GTGCAGAGAG CAGACCCCGT GTCCCGTTAC 
CGCTTCTCCC TCAGTGCCAG GACGCAGGTG GGCTCTGGAG AAGCAGCCAC GGAGGAGTCC 
CCAACACCTC CAAATGAAGC TACTCCAACT GCAGCTCCTC CCACATTGCC CCCGACTACC 
GTGGGTACCA CAGGCCTTGT GAGCAGTACT GATGCTACTG CCCTTGCTGC CACCAGTGAA 
GCCACAACAG TTCCCATCAT CCCAACCGTC GTACCTACCA CCGTCGCCAC CACCATTGCC 
ACAACTACTA CAACCACTGC CGCCGCCACC ACCACTACCA CCACGGAGAG TCCTCCCACT 
ACCACCACTG GGACTAAGAT TCACGAAACC GCCCCCGACG AGCAGTCCAT TTGGAACGTC 
ACGGTGCTCC CCAACAGTAA ATGGGCCAAC ATCACCTGGA AGCACAATTT CAGGCCTGGA 
ACTGACTTTG TGGTTGAGTA CATCGACAGC AACCATACGA AAAAAACTGT CCCTGTTAAG 
GCCCAAGCCC AGCCCATACA GCTGACAGAC CTCTTTCCCG GGATGACGTA CACGTTGCGG 
GTGTATTCCC GGGACAACGA GGGCATCAGC AGTACCGTCA TCACCTTTAT GACCAGTACA 
GCTTACACCA ACAACCAGAC TGACATCGCC ACCCAGGGCT GGTTCATCGG GCTCATGTGT 
GCCATTGCCC TTCTGGTGCT GATCCTGCTT ATCGTCTGCT TCATCAAGAG GAGTCGTGGC 
GGCAAGTACC CAGTGCGAGA AAAGAAGGAT GTCCCCTTGG GCCCTGAAGA CCCCAAAGAA 
GAAGATGGTT CATTTGACTA CAGTGACGAG GACAACAAGC CCCTGCAGGG CAGCCAGACA 
TCTCTGGACG GCACCATCAA GCAGCAGGAG AGTGACGACA GCCTAGTGGA CTACGGCGAG 
GGTGGCGAGG GCCAGTTCAA CGAAGATGGC TCCTTTATTG GCCAGTACAC TGTCAGAAAG 
GACAAGGAGG AGACCGAGGG CAATGAGAGC TCAGAGGCCA CATCTCCAGT CAATGCCATC 
TATTCCCTGG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454140.1
CDS	291..4385
Translation	MARQQAPPWVHVALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWLVNGDPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPETVIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVGRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPTPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAAATTTTTTESPPTTTTGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQTDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVRKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017598651.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurofascin (Nfasc), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598651.1

ORF Insert Sequence:

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACGTAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATTGAGAT TCCGATGGAT CCAAGCATTC AGAATGAGCT GACCCAACCC 
CCAACGATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ACCCCCGAGA TAACATCCTG 
ATTGAATGTG AAGCTAAAGG GAACCCCGCC CCCAGTTTCC ACTGGACTCG CAACAGCAGG 
TTCTTCAACA TTGCCAAGGA CCCACGGGTG TCCATGAGGA GGAGGTCTGG GACCTTGGTG 
ATCGACTTCC GCAGTGGTGG GCGGCCTGAG GAGTACGAAG GGGAGTACCA GTGCTTTGCC 
CGGAACAAAT TCGGCACAGC TCTTAGCAAC CGCATCCGCC TGCAGGTGTC CAAATCTCCC 
CTGTGGCCCA AGGAAAACCT AGACCCCGTC GTGGTTCAAG AGGGTGCCCC CTTAACCCTG 
CAGTGCAACC CCCCACCTGG CCTCCCATCC CCCGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAGGACAA GCGTGTCTCC CAGGGTCACA ACGGGGACCT GTACTTCTCC 
AACGTCATGC TGCAGGACAT GCAGACCGAC TACAGCTGCA ATGCACGCTT CCACTTCACC 
CACACCATTC AGCAGAAGAA TCCCTTCACC CTCAAAGTCC TCACCAACAA CCCCTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATA TATAGTGCCC GAGGAGTTGC GGAAAGAACA 
CCTAGCTTCA TGTATCCCCA GGGCACGTCA AGCAGTCAGA TGGTACTGCG CGGCATGGAC 
CTGCTGCTGG AGTGCATTGC CTCTGGGGTC CCAACACCAG ATATTGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTCGAGAATT TTAACAAGGC TCTGCGCATC 
ACCAATGTCT CTGAAGAGGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCCC CATACTGGCT GGATGAGCCC 
AAGAACTTGA TCCTGGCTCC TGGTGAAGAT GGGAGGCTGG TGTGTCGAGC CAATGGGAAC 
CCGAAGCCGA CCGTCCAGTG GCTGGTGAAT GGAGACCCTT TGCAATCGGC ACCACCCAAC 
CCCAACCGTG AGGTGGCCGG AGACACTATC ATCTTCCGGG ACACTCAGAT CAGCAGCAGG 
GCAGTGTACC AGTGTAACAC ATCCAACGAA CATGGCTACC TGCTGGCCAA TGCCTTCGTC 
AGCGTATTAG ATGTACCCCC TCGGATGCTG TCTCCCCGGA ACCAGCTCAT CAGGGTGATC 
CTTTACAACC GGACGCGACT GGACTGTCCG TTCTTTGGGT CTCCCATTCC AACACTCCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGTA ACTACCACGT CTACGAAAAT 
GGCAGCCTGG AAATCAAGAT GATTCGCAAA GAGGACCAAG GCATCTACAC CTGTGTGGCC 
ACCAACATCC TGGGAAAAGC TGAAAATCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACAGGA TGCCTGAAGA CCAGGTGGCC AAGAGGGGCA CCACAGTGCA GCTGGAGTGC 
CGTGTGAAGC ATGACCCCTC CTTGAAACTC ACAGTCTCCT GGCTGAAGGA TGACGAGCCA 
CTCTACATTG GAAACAGGAT GAAAAAGGAA GATGACTCCC TGACCATCTT CGGAGTGGCA 
GAGCGGGACC AAGGCAGTTA CACGTGCATG GCCAGCACCG AGCTGGACCA GGACCTGGCA 
AAGGCCTACC TCACTGTTCT AGCTGATCAG GCCACTCCGA CTAACCGTTT GGCTGCCCTG 
CCCAAAGGGC GGCCAGACCG ACCCAGGGAC CTGGAGCTCA CTGACCTGGC GGAAAGGAGT 
GTGAGGCTGA CCTGGATCCC GGGGGATGAT AACAACAGTC CCATCACAGA CTACGTTGTC 
CAGTTTGAAG AGGACCAGTT CCAGCCAGGA GTCTGGCATG ACCACTCCAA GTTCCCAGGC 
AGTGTCAACT CAGCCGTCCT CCATCTGTCC CCGTATGTCA ACTATCAGTT CCGAGTCATC 
GCTGTCAACG AGGTTGGGAG CAGCCACCCC AGCCTTCCAT CCGAGCGGTA CCGAACAAGC 
GGGGCACCCC CTGAATCCAA CCCCAGTGAT GTGAAGGGCG AAGGGACACG AAAAAACAAT 
ATGGAGATCA CATGGACGCC TATGAATGCT ACCTCCGCCT TTGGCCCCAA CCTGCGCTAC 
ATTGTCAAGT GGCGACGGAG AGAAACCCGA GAGACTTGGA ACAATGTCAC CGTGTGGGGC 
TCTCGCTATG TGGTGGGGCA GACCCCTGTC TACGTACCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TTGGGAAAGG CCCGGAGCCT GAAACCGTCA TTGGGTACTC GGGGGAAGAT 
TATCCCAGGG CTGCACCCAC TGAAGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGC 
CTTCAGTGGA ACCGCGTCTA CTCTGACACG GTCCAGGGCC AGCTCAGAGA GTATCGAGCT 
TACTACTGGA GGGAAAGCAG TTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAG 
GCCAGCTTCC CTGGCGACCG CCCCCGGGGC GTGGTGGGTC GCCTGTTCCC CTACAGTAAC 
TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGTGATG GGCCTCGAAG TGAAACCAAG 
GAATTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGGC GTTTCAGAGT CCGACAGCCC 
AACCTGGAGA CCATCAACCT GGAATGGGAT CACCCAGAGC ACCCCAACGG GATCCTGATT 
GGATACACGC TCAGATACGT GCCCTTTAAT GGAACCAAAC TGGGAAAGCA GATGGTGGAA 
AACTTCTCTC CCAATCAGAC CAAGTTCTCG GTGCAGAGAG CAGACCCCGT GTCCCGTTAC 
CGCTTCTCCC TCAGTGCCAG GACGCAGGTG GGCTCTGGAG AAGCAGCCAC GGAGGAGTCC 
CCAACACCTC CAAATGAAGC TACTCCAACT GCAGCTCCTC CCACATTGCC CCCGACTACC 
GTGGGTACCA CAGGCCTTGT GAGCAGTACT GATGCTACTG CCCTTGCTGC CACCAGTGAA 
GCCACAACAG TTCCCATCAT CCCAACCGTC GTACCTACCA CCGTCGCCAC CACCATTGCC 
ACAACTACTA CAACCACTGC CGCCGCCACC ACCACTACCA CCACGGAGAG TCCTCCCACT 
ACCACCACTG GGACTAAGAT TCACGAAACC GCCCCCGACG AGCAGTCCAT TTGGAACGTC 
ACGGTGCTCC CCAACAGTAA ATGGGCCAAC ATCACCTGGA AGCACAATTT CAGGCCTGGA 
ACTGACTTTG TGGTTGAGTA CATCGACAGC AACCATACGA AAAAAACTGT CCCTGTTAAG 
GCCCAAGCCC AGCCCATACA GCTGACAGAC CTCTTTCCCG GGATGACGTA CACGTTGCGG 
GTGTATTCCC GGGACAACGA GGGCATCAGC AGTACCGTCA TCACCTTTAT GACCAGTACA 
GCTTACACCA ACAACCAGAC TGACATCGCC ACCCAGGGCT GGTTCATCGG GCTCATGTGT 
GCCATTGCCC TTCTGGTGCT GATCCTGCTT ATCGTCTGCT TCATCAAGAG GAGTCGTGGC 
GGCAAGTACC CAGTGCGAGA AAAGAAGGAT GTCCCCTTGG GCCCTGAAGA CCCCAAAGAA 
GAAGATGGTT CATTTGACTA CAGTGACGAG GACAACAAGC CCCTGCAGGG CAGCCAGACA 
TCTCTGGACG GCACCATCAA GCAGCAGGAG AGTGACGACA GCCTAGTGGA CTACGGCGAG 
GGTGGCGAGG GCCAGTTCAA CGAAGATGGC TCCTTTATTG GCCAGTACAC TGTCAGAAAG 
GACAAGGAGG AGACCGAGGG CAATGAGAGC TCAGAGGCCA CATCTCCAGT CAATGCCATC 
TATTCCCTGG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfasc ( XM_017598652.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454141.1 Rattus norvegicus neurofascin (Nfasc), transcript variant X18, mRNA.

Price & Availability↑

CloneID	ORa35285
Clone ID Related Accession (Same CDS sequence)	XM_017598651.1 , XM_017598652.1
Accession Version	XM_017598652.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neurofascin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACGTAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATTGAGAT TCCGATGGAT CCAAGCATTC AGAATGAGCT GACCCAACCC 
CCAACGATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ACCCCCGAGA TAACATCCTG 
ATTGAATGTG AAGCTAAAGG GAACCCCGCC CCCAGTTTCC ACTGGACTCG CAACAGCAGG 
TTCTTCAACA TTGCCAAGGA CCCACGGGTG TCCATGAGGA GGAGGTCTGG GACCTTGGTG 
ATCGACTTCC GCAGTGGTGG GCGGCCTGAG GAGTACGAAG GGGAGTACCA GTGCTTTGCC 
CGGAACAAAT TCGGCACAGC TCTTAGCAAC CGCATCCGCC TGCAGGTGTC CAAATCTCCC 
CTGTGGCCCA AGGAAAACCT AGACCCCGTC GTGGTTCAAG AGGGTGCCCC CTTAACCCTG 
CAGTGCAACC CCCCACCTGG CCTCCCATCC CCCGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAGGACAA GCGTGTCTCC CAGGGTCACA ACGGGGACCT GTACTTCTCC 
AACGTCATGC TGCAGGACAT GCAGACCGAC TACAGCTGCA ATGCACGCTT CCACTTCACC 
CACACCATTC AGCAGAAGAA TCCCTTCACC CTCAAAGTCC TCACCAACAA CCCCTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATA TATAGTGCCC GAGGAGTTGC GGAAAGAACA 
CCTAGCTTCA TGTATCCCCA GGGCACGTCA AGCAGTCAGA TGGTACTGCG CGGCATGGAC 
CTGCTGCTGG AGTGCATTGC CTCTGGGGTC CCAACACCAG ATATTGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTCGAGAATT TTAACAAGGC TCTGCGCATC 
ACCAATGTCT CTGAAGAGGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCCC CATACTGGCT GGATGAGCCC 
AAGAACTTGA TCCTGGCTCC TGGTGAAGAT GGGAGGCTGG TGTGTCGAGC CAATGGGAAC 
CCGAAGCCGA CCGTCCAGTG GCTGGTGAAT GGAGACCCTT TGCAATCGGC ACCACCCAAC 
CCCAACCGTG AGGTGGCCGG AGACACTATC ATCTTCCGGG ACACTCAGAT CAGCAGCAGG 
GCAGTGTACC AGTGTAACAC ATCCAACGAA CATGGCTACC TGCTGGCCAA TGCCTTCGTC 
AGCGTATTAG ATGTACCCCC TCGGATGCTG TCTCCCCGGA ACCAGCTCAT CAGGGTGATC 
CTTTACAACC GGACGCGACT GGACTGTCCG TTCTTTGGGT CTCCCATTCC AACACTCCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGTA ACTACCACGT CTACGAAAAT 
GGCAGCCTGG AAATCAAGAT GATTCGCAAA GAGGACCAAG GCATCTACAC CTGTGTGGCC 
ACCAACATCC TGGGAAAAGC TGAAAATCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACAGGA TGCCTGAAGA CCAGGTGGCC AAGAGGGGCA CCACAGTGCA GCTGGAGTGC 
CGTGTGAAGC ATGACCCCTC CTTGAAACTC ACAGTCTCCT GGCTGAAGGA TGACGAGCCA 
CTCTACATTG GAAACAGGAT GAAAAAGGAA GATGACTCCC TGACCATCTT CGGAGTGGCA 
GAGCGGGACC AAGGCAGTTA CACGTGCATG GCCAGCACCG AGCTGGACCA GGACCTGGCA 
AAGGCCTACC TCACTGTTCT AGCTGATCAG GCCACTCCGA CTAACCGTTT GGCTGCCCTG 
CCCAAAGGGC GGCCAGACCG ACCCAGGGAC CTGGAGCTCA CTGACCTGGC GGAAAGGAGT 
GTGAGGCTGA CCTGGATCCC GGGGGATGAT AACAACAGTC CCATCACAGA CTACGTTGTC 
CAGTTTGAAG AGGACCAGTT CCAGCCAGGA GTCTGGCATG ACCACTCCAA GTTCCCAGGC 
AGTGTCAACT CAGCCGTCCT CCATCTGTCC CCGTATGTCA ACTATCAGTT CCGAGTCATC 
GCTGTCAACG AGGTTGGGAG CAGCCACCCC AGCCTTCCAT CCGAGCGGTA CCGAACAAGC 
GGGGCACCCC CTGAATCCAA CCCCAGTGAT GTGAAGGGCG AAGGGACACG AAAAAACAAT 
ATGGAGATCA CATGGACGCC TATGAATGCT ACCTCCGCCT TTGGCCCCAA CCTGCGCTAC 
ATTGTCAAGT GGCGACGGAG AGAAACCCGA GAGACTTGGA ACAATGTCAC CGTGTGGGGC 
TCTCGCTATG TGGTGGGGCA GACCCCTGTC TACGTACCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TTGGGAAAGG CCCGGAGCCT GAAACCGTCA TTGGGTACTC GGGGGAAGAT 
TATCCCAGGG CTGCACCCAC TGAAGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGC 
CTTCAGTGGA ACCGCGTCTA CTCTGACACG GTCCAGGGCC AGCTCAGAGA GTATCGAGCT 
TACTACTGGA GGGAAAGCAG TTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAG 
GCCAGCTTCC CTGGCGACCG CCCCCGGGGC GTGGTGGGTC GCCTGTTCCC CTACAGTAAC 
TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGTGATG GGCCTCGAAG TGAAACCAAG 
GAATTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGGC GTTTCAGAGT CCGACAGCCC 
AACCTGGAGA CCATCAACCT GGAATGGGAT CACCCAGAGC ACCCCAACGG GATCCTGATT 
GGATACACGC TCAGATACGT GCCCTTTAAT GGAACCAAAC TGGGAAAGCA GATGGTGGAA 
AACTTCTCTC CCAATCAGAC CAAGTTCTCG GTGCAGAGAG CAGACCCCGT GTCCCGTTAC 
CGCTTCTCCC TCAGTGCCAG GACGCAGGTG GGCTCTGGAG AAGCAGCCAC GGAGGAGTCC 
CCAACACCTC CAAATGAAGC TACTCCAACT GCAGCTCCTC CCACATTGCC CCCGACTACC 
GTGGGTACCA CAGGCCTTGT GAGCAGTACT GATGCTACTG CCCTTGCTGC CACCAGTGAA 
GCCACAACAG TTCCCATCAT CCCAACCGTC GTACCTACCA CCGTCGCCAC CACCATTGCC 
ACAACTACTA CAACCACTGC CGCCGCCACC ACCACTACCA CCACGGAGAG TCCTCCCACT 
ACCACCACTG GGACTAAGAT TCACGAAACC GCCCCCGACG AGCAGTCCAT TTGGAACGTC 
ACGGTGCTCC CCAACAGTAA ATGGGCCAAC ATCACCTGGA AGCACAATTT CAGGCCTGGA 
ACTGACTTTG TGGTTGAGTA CATCGACAGC AACCATACGA AAAAAACTGT CCCTGTTAAG 
GCCCAAGCCC AGCCCATACA GCTGACAGAC CTCTTTCCCG GGATGACGTA CACGTTGCGG 
GTGTATTCCC GGGACAACGA GGGCATCAGC AGTACCGTCA TCACCTTTAT GACCAGTACA 
GCTTACACCA ACAACCAGAC TGACATCGCC ACCCAGGGCT GGTTCATCGG GCTCATGTGT 
GCCATTGCCC TTCTGGTGCT GATCCTGCTT ATCGTCTGCT TCATCAAGAG GAGTCGTGGC 
GGCAAGTACC CAGTGCGAGA AAAGAAGGAT GTCCCCTTGG GCCCTGAAGA CCCCAAAGAA 
GAAGATGGTT CATTTGACTA CAGTGACGAG GACAACAAGC CCCTGCAGGG CAGCCAGACA 
TCTCTGGACG GCACCATCAA GCAGCAGGAG AGTGACGACA GCCTAGTGGA CTACGGCGAG 
GGTGGCGAGG GCCAGTTCAA CGAAGATGGC TCCTTTATTG GCCAGTACAC TGTCAGAAAG 
GACAAGGAGG AGACCGAGGG CAATGAGAGC TCAGAGGCCA CATCTCCAGT CAATGCCATC 
TATTCCCTGG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454141.1
CDS	287..4381
Translation	MARQQAPPWVHVALILFLLSLGGAIEIPMDPSIQNELTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECIASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWLVNGDPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNRLAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSKFPGSVNSAVLHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPETVIGYSGEDYPRAAPTEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVGRLFPYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYTLRYVPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPTPPNEATPTAAPPTLPPTTVGTTGLVSSTDATALAATSEATTVPIIPTVVPTTVATTIATTTTTTAAATTTTTTESPPTTTTGTKIHETAPDEQSIWNVTVLPNSKWANITWKHNFRPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLFPGMTYTLRVYSRDNEGISSTVITFMTSTAYTNNQTDIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVRKDKEETEGNESSEATSPVNAIYSLA

Target ORF information:

RefSeq Version	XM_017598652.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurofascin (Nfasc), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598652.1

ORF Insert Sequence:

ATGGCCAGGC AGCAGGCGCC ACCCTGGGTC CACGTAGCCC TCATCCTCTT CCTCCTCAGC 
CTCGGAGGGG CCATTGAGAT TCCGATGGAT CCAAGCATTC AGAATGAGCT GACCCAACCC 
CCAACGATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ACCCCCGAGA TAACATCCTG 
ATTGAATGTG AAGCTAAAGG GAACCCCGCC CCCAGTTTCC ACTGGACTCG CAACAGCAGG 
TTCTTCAACA TTGCCAAGGA CCCACGGGTG TCCATGAGGA GGAGGTCTGG GACCTTGGTG 
ATCGACTTCC GCAGTGGTGG GCGGCCTGAG GAGTACGAAG GGGAGTACCA GTGCTTTGCC 
CGGAACAAAT TCGGCACAGC TCTTAGCAAC CGCATCCGCC TGCAGGTGTC CAAATCTCCC 
CTGTGGCCCA AGGAAAACCT AGACCCCGTC GTGGTTCAAG AGGGTGCCCC CTTAACCCTG 
CAGTGCAACC CCCCACCTGG CCTCCCATCC CCCGTCATCT TCTGGATGAG CAGCTCCATG 
GAGCCCATCA CCCAGGACAA GCGTGTCTCC CAGGGTCACA ACGGGGACCT GTACTTCTCC 
AACGTCATGC TGCAGGACAT GCAGACCGAC TACAGCTGCA ATGCACGCTT CCACTTCACC 
CACACCATTC AGCAGAAGAA TCCCTTCACC CTCAAAGTCC TCACCAACAA CCCCTATAAT 
GACTCGTCCT TAAGAAACCA CCCTGACATA TATAGTGCCC GAGGAGTTGC GGAAAGAACA 
CCTAGCTTCA TGTATCCCCA GGGCACGTCA AGCAGTCAGA TGGTACTGCG CGGCATGGAC 
CTGCTGCTGG AGTGCATTGC CTCTGGGGTC CCAACACCAG ATATTGCATG GTACAAGAAA 
GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTCGAGAATT TTAACAAGGC TCTGCGCATC 
ACCAATGTCT CTGAAGAGGA CTCTGGGGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC 
AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCCC CATACTGGCT GGATGAGCCC 
AAGAACTTGA TCCTGGCTCC TGGTGAAGAT GGGAGGCTGG TGTGTCGAGC CAATGGGAAC 
CCGAAGCCGA CCGTCCAGTG GCTGGTGAAT GGAGACCCTT TGCAATCGGC ACCACCCAAC 
CCCAACCGTG AGGTGGCCGG AGACACTATC ATCTTCCGGG ACACTCAGAT CAGCAGCAGG 
GCAGTGTACC AGTGTAACAC ATCCAACGAA CATGGCTACC TGCTGGCCAA TGCCTTCGTC 
AGCGTATTAG ATGTACCCCC TCGGATGCTG TCTCCCCGGA ACCAGCTCAT CAGGGTGATC 
CTTTACAACC GGACGCGACT GGACTGTCCG TTCTTTGGGT CTCCCATTCC AACACTCCGA 
TGGTTTAAGA ATGGGCAAGG AAGCAACCTG GATGGTGGTA ACTACCACGT CTACGAAAAT 
GGCAGCCTGG AAATCAAGAT GATTCGCAAA GAGGACCAAG GCATCTACAC CTGTGTGGCC 
ACCAACATCC TGGGAAAAGC TGAAAATCAA GTCCGCCTGG AGGTCAAAGA CCCCACCAGG 
ATCTACAGGA TGCCTGAAGA CCAGGTGGCC AAGAGGGGCA CCACAGTGCA GCTGGAGTGC 
CGTGTGAAGC ATGACCCCTC CTTGAAACTC ACAGTCTCCT GGCTGAAGGA TGACGAGCCA 
CTCTACATTG GAAACAGGAT GAAAAAGGAA GATGACTCCC TGACCATCTT CGGAGTGGCA 
GAGCGGGACC AAGGCAGTTA CACGTGCATG GCCAGCACCG AGCTGGACCA GGACCTGGCA 
AAGGCCTACC TCACTGTTCT AGCTGATCAG GCCACTCCGA CTAACCGTTT GGCTGCCCTG 
CCCAAAGGGC GGCCAGACCG ACCCAGGGAC CTGGAGCTCA CTGACCTGGC GGAAAGGAGT 
GTGAGGCTGA CCTGGATCCC GGGGGATGAT AACAACAGTC CCATCACAGA CTACGTTGTC 
CAGTTTGAAG AGGACCAGTT CCAGCCAGGA GTCTGGCATG ACCACTCCAA GTTCCCAGGC 
AGTGTCAACT CAGCCGTCCT CCATCTGTCC CCGTATGTCA ACTATCAGTT CCGAGTCATC 
GCTGTCAACG AGGTTGGGAG CAGCCACCCC AGCCTTCCAT CCGAGCGGTA CCGAACAAGC 
GGGGCACCCC CTGAATCCAA CCCCAGTGAT GTGAAGGGCG AAGGGACACG AAAAAACAAT 
ATGGAGATCA CATGGACGCC TATGAATGCT ACCTCCGCCT TTGGCCCCAA CCTGCGCTAC 
ATTGTCAAGT GGCGACGGAG AGAAACCCGA GAGACTTGGA ACAATGTCAC CGTGTGGGGC 
TCTCGCTATG TGGTGGGGCA GACCCCTGTC TACGTACCCT ATGAGATCCG AGTCCAGGCT 
GAAAATGACT TTGGGAAAGG CCCGGAGCCT GAAACCGTCA TTGGGTACTC GGGGGAAGAT 
TATCCCAGGG CTGCACCCAC TGAAGTTAAA ATCCGAGTCC TGAACAGCAC AGCCATCAGC 
CTTCAGTGGA ACCGCGTCTA CTCTGACACG GTCCAGGGCC AGCTCAGAGA GTATCGAGCT 
TACTACTGGA GGGAAAGCAG TTTGCTGAAG AACCTGTGGG TGTCTCAGAA GAGACAGCAG 
GCCAGCTTCC CTGGCGACCG CCCCCGGGGC GTGGTGGGTC GCCTGTTCCC CTACAGTAAC 
TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGTGATG GGCCTCGAAG TGAAACCAAG 
GAATTCACCA CCCCGGAAGG AGTACCCAGT GCCCCCAGGC GTTTCAGAGT CCGACAGCCC 
AACCTGGAGA CCATCAACCT GGAATGGGAT CACCCAGAGC ACCCCAACGG GATCCTGATT 
GGATACACGC TCAGATACGT GCCCTTTAAT GGAACCAAAC TGGGAAAGCA GATGGTGGAA 
AACTTCTCTC CCAATCAGAC CAAGTTCTCG GTGCAGAGAG CAGACCCCGT GTCCCGTTAC 
CGCTTCTCCC TCAGTGCCAG GACGCAGGTG GGCTCTGGAG AAGCAGCCAC GGAGGAGTCC 
CCAACACCTC CAAATGAAGC TACTCCAACT GCAGCTCCTC CCACATTGCC CCCGACTACC 
GTGGGTACCA CAGGCCTTGT GAGCAGTACT GATGCTACTG CCCTTGCTGC CACCAGTGAA 
GCCACAACAG TTCCCATCAT CCCAACCGTC GTACCTACCA CCGTCGCCAC CACCATTGCC 
ACAACTACTA CAACCACTGC CGCCGCCACC ACCACTACCA CCACGGAGAG TCCTCCCACT 
ACCACCACTG GGACTAAGAT TCACGAAACC GCCCCCGACG AGCAGTCCAT TTGGAACGTC 
ACGGTGCTCC CCAACAGTAA ATGGGCCAAC ATCACCTGGA AGCACAATTT CAGGCCTGGA 
ACTGACTTTG TGGTTGAGTA CATCGACAGC AACCATACGA AAAAAACTGT CCCTGTTAAG 
GCCCAAGCCC AGCCCATACA GCTGACAGAC CTCTTTCCCG GGATGACGTA CACGTTGCGG 
GTGTATTCCC GGGACAACGA GGGCATCAGC AGTACCGTCA TCACCTTTAT GACCAGTACA 
GCTTACACCA ACAACCAGAC TGACATCGCC ACCCAGGGCT GGTTCATCGG GCTCATGTGT 
GCCATTGCCC TTCTGGTGCT GATCCTGCTT ATCGTCTGCT TCATCAAGAG GAGTCGTGGC 
GGCAAGTACC CAGTGCGAGA AAAGAAGGAT GTCCCCTTGG GCCCTGAAGA CCCCAAAGAA 
GAAGATGGTT CATTTGACTA CAGTGACGAG GACAACAAGC CCCTGCAGGG CAGCCAGACA 
TCTCTGGACG GCACCATCAA GCAGCAGGAG AGTGACGACA GCCTAGTGGA CTACGGCGAG 
GGTGGCGAGG GCCAGTTCAA CGAAGATGGC TCCTTTATTG GCCAGTACAC TGTCAGAAAG 
GACAAGGAGG AGACCGAGGG CAATGAGAGC TCAGAGGCCA CATCTCCAGT CAATGCCATC 
TATTCCCTGG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Nfasc
Entrez Gene ID	116690
Full Name	neurofascin
Synonyms	NF
General protein information	Preferred Names neurofascin Names neurofascin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q13
Summary	This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined.[provided by RefSeq, May 2009].

Mus musculus (house mouse)	Nfasc	NP_874385.1
Xenopus tropicalis (tropical clawed frog)	nfasc	XP_004910755.1
Rattus norvegicus (Norway rat)	Nfasc	NP_001153786.1
Danio rerio (zebrafish)	nfasca	NP_001139239.1
	NFASC	XP_001096628.1
Canis lupus familiaris (dog)	NFASC	XP_005640834.1
Pan troglodytes (chimpanzee)	NFASC	XP_003308755.1
Gallus gallus (chicken)	NFASC	NP_001153794.1
Homo sapiens (human)	NFASC	NP_001005388.2
Bos taurus (cattle)	NFASC	XP_005216764.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Nfasc cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

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