Plaa cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Plaa
Entrez Gene ID	116645
Full Name	phospholipase A2, activating protein
Synonyms	PLA2P,Plap
General protein information	Preferred Names phospholipase A2, activating protein Names phospholipase A-2-activating protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q33
Summary	may play a role in phospholipase activation, production of prostaglandins, and regulation of inflammatory response [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053866.3	NP_446318.3	phospholipase A-2-activating protein

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	PLAA	XP_424941.2
Danio rerio (zebrafish)	plaa	NP_997855.1
Schizosaccharomyces pombe (fission yeast)	lub1	NP_596478.2
Arabidopsis thaliana (thale cress)	AT3G18860	NP_850612.1
Homo sapiens (human)	PLAA	NP_001026859.1
Drosophila melanogaster (fruit fly)	Plap	NP_001245829.1
Neurospora crassa	NCU00880	XP_964796.2
Canis lupus familiaris (dog)	PLAA	XP_531963.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008620	XP_314716.4
	PLAA	XP_001105698.1
Bos taurus (cattle)	PLAA	NP_001098805.1
Saccharomyces cerevisiae (baker's yeast)	DOA1	NP_012709.1
Eremothecium gossypii	AGOS_AER448W	NP_985303.2
Pan troglodytes (chimpanzee)	PLAA	XP_520517.2
Mus musculus (house mouse)	Plaa	NP_766283.2
Rattus norvegicus (Norway rat)	Plaa	NP_446318.3
Kluyveromyces lactis	KLLA0B14410g	XP_452172.1
Magnaporthe oryzae (rice blast fungus)	MGG_14014	XP_003721359.1
Xenopus tropicalis (tropical clawed frog)	plaa	NP_001116275.1

Gene Ontology
Bibliography

Related articles in PubMed

PLAA Mutations Cause a Lethal Infantile Epileptic Encephalopathy by Disrupting Ubiquitin-Mediated Endolysosomal Degradation of Synaptic Proteins.
Hall EA, Nahorski MS, Murray LM, Shaheen R, Perkins E, Dissanayake KN, Kristaryanto Y, Jones RA, Vogt J, Rivagorda M, Handley MT, Mali GR, Quidwai T, Soares DC, Keighren MA, McKie L, Mort RL, Gammoh N, Garcia-Munoz A, Davey T, Vermeren M, Walsh D, Budd P, Aligianis IA, Faqeih E, Quigley AJ, Jackson IJ, Kulathu Y, Jackson M, Ribchester RR, von Kriegsheim A, Alkuraya FS, Woods CG, Maher ER, Mill P
American journal of human genetics100(5)706-724(2017 May)

Phospholipase A2-activating protein is associated with a novel form of leukoencephalopathy.
Falik Zaccai TC, Savitzki D, Zivony-Elboum Y, Vilboux T, Fitts EC, Shoval Y, Kalfon L, Samra N, Keren Z, Gross B, Chasnyk N, Straussberg R, Mullikin JC, Teer JK, Geiger D, Kornitzer D, Bitterman-Deutsch O, Samson AO, Wakamiya M, Peterson JW, Kirtley ML, Pinchuk IV, Baze WB, Gahl WA, Kleta R, Anikster Y, Chopra AK
Brain : a journal of neurology140(2)370-386(2017 02)

VCP/p97 cooperates with YOD1, UBXD1 and PLAA to drive clearance of ruptured lysosomes by autophagy.
Papadopoulos C, Kirchner P, Bug M, Grum D, Koerver L, Schulze N, Poehler R, Dressler A, Fengler S, Arhzaouy K, Lux V, Ehrmann M, Weihl CC, Meyer H
The EMBO journal36(2)135-150(2017 01)

Cytosolic phospholipase A2 protein as a novel therapeutic target for spinal cord injury.
Liu NK, Deng LX, Zhang YP, Lu QB, Wang XF, Hu JG, Oakes E, Bonventre JV, Shields CB, Xu XM
Annals of neurology75(5)644-58(2014 May)

Specific isolation of placenta-derived exosomes from the circulation of pregnant women and their immunoregulatory consequences.
Sabapatha A, Gercel-Taylor C, Taylor DD
American journal of reproductive immunology (New York, N.Y. : 1989)56(5-6)345-55(2006 Nov-Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cytosolic phospholipase A2 mRNA is expressed in rat injured spinal cord at 7 days after spinal cord injury.
Title: Cytosolic phospholipase A2 protein as a novel therapeutic target for spinal cord injury.

These data suggest that the ANG II-induced neointimal growth is mediated by the activation of Akt through a mechanism dependent on cPLA2 and PLD2 activation in balloon-injured rat carotid arteries.
Title: ANG II-induced neointimal growth is mediated via cPLA2- and PLD2-activated Akt in balloon-injured rat carotid artery.

PLAA is a specific activator of PLA2 in growth plate chondrocytes, suggesting that it mediates the membrane effect of 1alpha,25(OH)2D3, thereby modulating physiological response.
Title: Phospholipase A2 activating protein (PLAA) is required for 1alpha,25(OH)2D3 signaling in growth plate chondrocytes.

The following Plaa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Plaa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10901	NM_053866.3 Latest version!	Rattus norvegicus phospholipase A2, activating protein (Plaa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Plaa ( NM_053866.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446318.3 Rattus norvegicus phospholipase A2, activating protein (Plaa), mRNA.

Price & Availability↑

CloneID	ORa10901
Clone ID Related Accession (Same CDS sequence)	NM_053866.3
Accession Version	NM_053866.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Rattus norvegicus(Norway rat)
Product	phospholipase A-2-activating protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CK366206.1, CV109083.1, CB701630.1, CV110320.1, CF110238.1 and BP498567.1. On Oct 17, 2007 this sequence version replaced NM_053866.2. ##Evidence-Data-START## Transcript exon combination :: FQ211509.1, FQ223782.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGAGCG GTGCCGCCAG ATACCGGCTG AGCTGCTCGC TCCCGGGCCA CGAACTGGAC 
GTGAGGGGCC TGGTGTGCTG CCTCTACCCA CCGGGAGCCT TTGTGTCTGT GTCCCGGGAT 
CGTACCACCC GCCTCTGGGC TCCAGACAGT CCTAACAGGG GCTTTACAGA AATGCACTGT 
ATGAGCGGCC ACTCTAATTT TGTGTCTTGT GTGTGCATCA TACCCTCAAG TGACATATAT 
CCTCATGGAC TAATTGCCAC TGGAGGAAAT GACCACAATA TTTGCATTTT CTCGCTGGAC 
AGTCCGATGC CACTTTATAT TTTAAAGGGT CACAAAGATA CTGTTTGTAG TCTTTCTTCT 
GGAAAATTTG GGACATTACT TAGTGGCTCA TGGGACACCA CTGCTAAAGT CTGGCTGAAT 
GACAAATGCA TGATGACATT ACAGGGTCAC ACAGCTGCAG TATGGGCAGT AAAGATTTTA 
CCTGAACAGG GCTTAATGTT AACTGGATCA GCAGACAAAA CAATTAAACT ATGGAAGGCC 
GGAAGATGTG AGAGGACTTT TTCAGGGCAT GAAGACTGTG TAAGAGGTTT GGCAATTTTG 
AGTGAAACAG AATTTCTTTC CTGTGCAAAT GATGCTAGTA TTAGAAGGTG GCAGATTACT 
GGCGAGTGTC TTGGAGTATA CTACGGACAT ACAAATTATA TTTATAGCAT ATCTGTCTTT 
CCAAATTGTA GAGATTTTGT GACAACTGCA GAAGACAGAT CTCTAAGAAT ATGGAAACAT 
GGTGAATGTG CCCAAACAAT CCGACTTCCA GCTCAGTCTA TATGGTGCTG CTGCGTACTG 
GACAATGGTG ACATTGTGGT TGGTGCGAGT GATGGTATTA TTAGAGTGTT TACAGAGGCA 
GACGAGCGGA CAGCCAGTGC TGAGGAAATC AAGGCTTTTG AGAGAGAGCT CTCTCAGGCA 
ACTATAGACT CCAAAACTGG CGACTTGGGG GACATTAATG CTGAGCAGCT TCCTGGGAGG 
GAACATCTGA ATGAGCCAGG TACTAGAGAA GGACAGACTC GTCTAATCAG AGATGGAGAG 
AGAGTTGAAG CCTATCAATG GAGTGTTAGT GATGGCAGGT GGATAAAAAT TGGTGATGTT 
GTTGGCTCAT CTGGTGCTAA TCAGCAAACA TCTGGAAAAG TTTTATATGA AGGGAAAGAA 
TTTGATTATG TTTTCTCCAT CGATGTGAAT GAAGGCGGAC CATCATATAA ATTACCATAT 
AATGTCAGTG ACGACCCCTG GCTGGTTGCA TACAACTTCT TGCAGAAAAA TGATTTGAAT 
CCCATGTTTT TGGATCAGGT GGCTAAATTT ATTATTGATA ACACAAAAGG TCAAACACTG 
GGACTTGGAA ATACCAGTTT TTCAGACCCA TTTACAGGTG GTGGTCGCTA TGTTCCAGGA 
ACTTCAGGGC CTTCCAACAC AGTGCAGACA GCAGATCCTT TCACAGGTGC TGGTCGTTAT 
ATGCCAGGGT CTGCAGGGAT GGATACCACC ATGGCTGGAG TTGATCCATT TACAGGAAAT 
AGTGCCTACC GATCAGCTGC GTCTAAAACA GTGAATATTT ATTTCCCCAA AAAAGAAGCT 
CTTACCTTCG ACCAAGCAAA CCCTACACAA ATATTAGGAA AACTGAAGGA ACTTAATGGA 
AGTGCACCTG AAGAGAAGAA GCTAACTGAA GATGACTTGG TACTTCTGGA AAAGATACTG 
TCCCTGATTT GTGGTAATGC TTCAGAAAAG CCGACGGCCC AGCAGCTTCA GGTTCTGTGG 
AAAGCCATCA ACTGGCCTGA AGACATTGTC TTTCCCGCAC TTGACATTCT TCGGCTATCA 
ATTAAGCATC CCAGTGTGAA TGAGAATTTC TGCAATGAAA AAGAAGGAGA CCAGTTCAGC 
AGCCACCTTA TCAACCTTCT GAACCCTAAA GGAAAGCCAG CAAACCAGCT GCTTGCTCTT 
AGGACTTTTT GCAATTGCTT TGTTAGTCAG GCAGGACAAA AGCTCATGAT GTCCCAGAGG 
GAGTCATTGA TGTCACATGC AATAGAACTG AAATCCGGGA GCAATAAGAA CATTCATATT 
GCTCTGGCTA CACTGACCTT GAACTATTCT GTTTGTTTCC ATAAAGACCA TAACATTGAA 
GGGAAAGCCC AATGCTTGTC AGTGATTAGC ACAATCTTGG AAGTTGTACA AGACCTAGAA 
GCCACATTTA GACTCCTTGT GGCTCTTGGG ACACTTATCA GTGATGATTC AAATGCTATA 
CAATTAGCCA AATCTTTAGG TGTTGATTCT CAAATAAAAA AGTATGCTTC TGTATCAGAG 
CCAGCTAAAG TAAGTGAATG CTGTAGACTT ATCTTAAATT TGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446318.3
CDS	114..2501
Translation	MASGAARYRLSCSLPGHELDVRGLVCCLYPPGAFVSVSRDRTTRLWAPDSPNRGFTEMHCMSGHSNFVSCVCIIPSSDIYPHGLIATGGNDHNICIFSLDSPMPLYILKGHKDTVCSLSSGKFGTLLSGSWDTTAKVWLNDKCMMTLQGHTAAVWAVKILPEQGLMLTGSADKTIKLWKAGRCERTFSGHEDCVRGLAILSETEFLSCANDASIRRWQITGECLGVYYGHTNYIYSISVFPNCRDFVTTAEDRSLRIWKHGECAQTIRLPAQSIWCCCVLDNGDIVVGASDGIIRVFTEADERTASAEEIKAFERELSQATIDSKTGDLGDINAEQLPGREHLNEPGTREGQTRLIRDGERVEAYQWSVSDGRWIKIGDVVGSSGANQQTSGKVLYEGKEFDYVFSIDVNEGGPSYKLPYNVSDDPWLVAYNFLQKNDLNPMFLDQVAKFIIDNTKGQTLGLGNTSFSDPFTGGGRYVPGTSGPSNTVQTADPFTGAGRYMPGSAGMDTTMAGVDPFTGNSAYRSAASKTVNIYFPKKEALTFDQANPTQILGKLKELNGSAPEEKKLTEDDLVLLEKILSLICGNASEKPTAQQLQVLWKAINWPEDIVFPALDILRLSIKHPSVNENFCNEKEGDQFSSHLINLLNPKGKPANQLLALRTFCNCFVSQAGQKLMMSQRESLMSHAIELKSGSNKNIHIALATLTLNYSVCFHKDHNIEGKAQCLSVISTILEVVQDLEATFRLLVALGTLISDDSNAIQLAKSLGVDSQIKKYASVSEPAKVSECCRLILNLL

Target ORF information:

RefSeq Version	NM_053866.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phospholipase A2, activating protein (Plaa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053866.3

ORF Insert Sequence:

ATGGCGAGCG GTGCCGCCAG ATACCGGCTG AGCTGCTCGC TCCCGGGCCA CGAACTGGAC 
GTGAGGGGCC TGGTGTGCTG CCTCTACCCA CCGGGAGCCT TTGTGTCTGT GTCCCGGGAT 
CGTACCACCC GCCTCTGGGC TCCAGACAGT CCTAACAGGG GCTTTACAGA AATGCACTGT 
ATGAGCGGCC ACTCTAATTT TGTGTCTTGT GTGTGCATCA TACCCTCAAG TGACATATAT 
CCTCATGGAC TAATTGCCAC TGGAGGAAAT GACCACAATA TTTGCATTTT CTCGCTGGAC 
AGTCCGATGC CACTTTATAT TTTAAAGGGT CACAAAGATA CTGTTTGTAG TCTTTCTTCT 
GGAAAATTTG GGACATTACT TAGTGGCTCA TGGGACACCA CTGCTAAAGT CTGGCTGAAT 
GACAAATGCA TGATGACATT ACAGGGTCAC ACAGCTGCAG TATGGGCAGT AAAGATTTTA 
CCTGAACAGG GCTTAATGTT AACTGGATCA GCAGACAAAA CAATTAAACT ATGGAAGGCC 
GGAAGATGTG AGAGGACTTT TTCAGGGCAT GAAGACTGTG TAAGAGGTTT GGCAATTTTG 
AGTGAAACAG AATTTCTTTC CTGTGCAAAT GATGCTAGTA TTAGAAGGTG GCAGATTACT 
GGCGAGTGTC TTGGAGTATA CTACGGACAT ACAAATTATA TTTATAGCAT ATCTGTCTTT 
CCAAATTGTA GAGATTTTGT GACAACTGCA GAAGACAGAT CTCTAAGAAT ATGGAAACAT 
GGTGAATGTG CCCAAACAAT CCGACTTCCA GCTCAGTCTA TATGGTGCTG CTGCGTACTG 
GACAATGGTG ACATTGTGGT TGGTGCGAGT GATGGTATTA TTAGAGTGTT TACAGAGGCA 
GACGAGCGGA CAGCCAGTGC TGAGGAAATC AAGGCTTTTG AGAGAGAGCT CTCTCAGGCA 
ACTATAGACT CCAAAACTGG CGACTTGGGG GACATTAATG CTGAGCAGCT TCCTGGGAGG 
GAACATCTGA ATGAGCCAGG TACTAGAGAA GGACAGACTC GTCTAATCAG AGATGGAGAG 
AGAGTTGAAG CCTATCAATG GAGTGTTAGT GATGGCAGGT GGATAAAAAT TGGTGATGTT 
GTTGGCTCAT CTGGTGCTAA TCAGCAAACA TCTGGAAAAG TTTTATATGA AGGGAAAGAA 
TTTGATTATG TTTTCTCCAT CGATGTGAAT GAAGGCGGAC CATCATATAA ATTACCATAT 
AATGTCAGTG ACGACCCCTG GCTGGTTGCA TACAACTTCT TGCAGAAAAA TGATTTGAAT 
CCCATGTTTT TGGATCAGGT GGCTAAATTT ATTATTGATA ACACAAAAGG TCAAACACTG 
GGACTTGGAA ATACCAGTTT TTCAGACCCA TTTACAGGTG GTGGTCGCTA TGTTCCAGGA 
ACTTCAGGGC CTTCCAACAC AGTGCAGACA GCAGATCCTT TCACAGGTGC TGGTCGTTAT 
ATGCCAGGGT CTGCAGGGAT GGATACCACC ATGGCTGGAG TTGATCCATT TACAGGAAAT 
AGTGCCTACC GATCAGCTGC GTCTAAAACA GTGAATATTT ATTTCCCCAA AAAAGAAGCT 
CTTACCTTCG ACCAAGCAAA CCCTACACAA ATATTAGGAA AACTGAAGGA ACTTAATGGA 
AGTGCACCTG AAGAGAAGAA GCTAACTGAA GATGACTTGG TACTTCTGGA AAAGATACTG 
TCCCTGATTT GTGGTAATGC TTCAGAAAAG CCGACGGCCC AGCAGCTTCA GGTTCTGTGG 
AAAGCCATCA ACTGGCCTGA AGACATTGTC TTTCCCGCAC TTGACATTCT TCGGCTATCA 
ATTAAGCATC CCAGTGTGAA TGAGAATTTC TGCAATGAAA AAGAAGGAGA CCAGTTCAGC 
AGCCACCTTA TCAACCTTCT GAACCCTAAA GGAAAGCCAG CAAACCAGCT GCTTGCTCTT 
AGGACTTTTT GCAATTGCTT TGTTAGTCAG GCAGGACAAA AGCTCATGAT GTCCCAGAGG 
GAGTCATTGA TGTCACATGC AATAGAACTG AAATCCGGGA GCAATAAGAA CATTCATATT 
GCTCTGGCTA CACTGACCTT GAACTATTCT GTTTGTTTCC ATAAAGACCA TAACATTGAA 
GGGAAAGCCC AATGCTTGTC AGTGATTAGC ACAATCTTGG AAGTTGTACA AGACCTAGAA 
GCCACATTTA GACTCCTTGT GGCTCTTGGG ACACTTATCA GTGATGATTC AAATGCTATA 
CAATTAGCCA AATCTTTAGG TGTTGATTCT CAAATAAAAA AGTATGCTTC TGTATCAGAG 
CCAGCTAAAG TAAGTGAATG CTGTAGACTT ATCTTAAATT TGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PLAA Mutations Cause a Lethal Infantile Epileptic Encephalopathy by Disrupting Ubiquitin-Mediated Endolysosomal Degradation of Synaptic Proteins.
American journal of human genetics100(5)706-724(2017 May)
Hall EA,Nahorski MS,Murray LM,Shaheen R,Perkins E,Dissanayake KN,Kristaryanto Y,Jones RA,Vogt J,Rivagorda M,Handley MT,Mali GR,Quidwai T,Soares DC,Keighren MA,McKie L,Mort RL,Gammoh N,Garcia-Munoz A,Davey T,Vermeren M,Walsh D,Budd P,Aligianis IA,Faqeih E,Quigley AJ,Jackson IJ,Kulathu Y,Jackson M,Ribchester RR,von Kriegsheim A,Alkuraya FS,Woods CG,Maher ER,Mill P

Phospholipase A2-activating protein is associated with a novel form of leukoencephalopathy.
Brain : a journal of neurology140(2)370-386(2017 02)
Falik Zaccai TC,Savitzki D,Zivony-Elboum Y,Vilboux T,Fitts EC,Shoval Y,Kalfon L,Samra N,Keren Z,Gross B,Chasnyk N,Straussberg R,Mullikin JC,Teer JK,Geiger D,Kornitzer D,Bitterman-Deutsch O,Samson AO,Wakamiya M,Peterson JW,Kirtley ML,Pinchuk IV,Baze WB,Gahl WA,Kleta R,Anikster Y,Chopra AK

VCP/p97 cooperates with YOD1, UBXD1 and PLAA to drive clearance of ruptured lysosomes by autophagy.
The EMBO journal36(2)135-150(2017 01)
Papadopoulos C,Kirchner P,Bug M,Grum D,Koerver L,Schulze N,Poehler R,Dressler A,Fengler S,Arhzaouy K,Lux V,Ehrmann M,Weihl CC,Meyer H

Cytosolic phospholipase A2 protein as a novel therapeutic target for spinal cord injury.
Annals of neurology75(5)644-58(2014 May)
Liu NK,Deng LX,Zhang YP,Lu QB,Wang XF,Hu JG,Oakes E,Bonventre JV,Shields CB,Xu XM

Specific isolation of placenta-derived exosomes from the circulation of pregnant women and their immunoregulatory consequences.
American journal of reproductive immunology (New York, N.Y. : 1989)56(5-6)345-55(2006 Nov-Dec)
Sabapatha A,Gercel-Taylor C,Taylor DD

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Plaa cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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