F2rl3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	F2rl3
Entrez Gene ID	116498
Full Name	F2R like thrombin or trypsin receptor 3
Synonyms	PAR4
General protein information	Preferred Names F2R like thrombin or trypsin receptor 3 Names proteinase-activated receptor 4 F2R like thrombin/trypsin receptor 3 PAR-4 coagulation factor II (thrombin) receptor-like 3 coagulation factor II receptor-like 3 protease-activated receptor 4 thrombin receptor-like 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16p14
Summary	G-protein-coupled receptor activated by thrombin and trypsin; mediates gastrointestinal motility [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053808.1	NP_446260.1	proteinase-activated receptor 4

Pathways from BioSystems

Homologies

Homo sapiens (human)	F2RL3	NP_003941.2
	F2RL3	XP_001112955.1
Bos taurus (cattle)	F2RL3	NP_001069606.1
Rattus norvegicus (Norway rat)	F2rl3	NP_446260.1
Pan troglodytes (chimpanzee)	F2RL3	XP_512484.2
Canis lupus familiaris (dog)	F2RL3	XP_541962.2
Mus musculus (house mouse)	F2rl3	NP_032001.2

Gene Ontology
Bibliography

Related articles in PubMed

Protease-activated receptor 1 and 4 signal inhibition reduces preterm neonatal hemorrhagic brain injury.
Lekic T, Klebe D, McBride DW, Manaenko A, Rolland WB, Flores JJ, Altay O, Tang J, Zhang JH
Stroke46(6)1710-3(2015 Jun)

Protease-activated receptor 4 activation increases the expression of calcitonin gene-related peptide mRNA and protein in dorsal root ganglion neurons.
Wang Z, Chen D, Zhang Z, Zhang R, An S, Yu L
Journal of neuroscience research91(12)1551-62(2013 Dec)

Protease activated receptors 1 and 4 sensitize TRPV1 in nociceptive neurones.
Vellani V, Kinsey AM, Prandini M, Hechtfischer SC, Reeh P, Magherini PC, Giacomoni C, McNaughton PA
Molecular pain661(2010 Sep)

Proteinase-activated receptor-4 (PAR4) activation leads to sensitization of rat joint primary afferents via a bradykinin B2 receptor-dependent mechanism.
Russell FA, Veldhoen VE, Tchitchkan D, McDougall JJ
Journal of neurophysiology103(1)155-63(2010 Jan)

The ATP-gated P2X1 receptor plays a pivotal role in activation of aspirin-treated platelets by thrombin and epinephrine.
Greneg?rd M, Vretenbrant-Oberg K, Nylander M, D?silets S, Lindstr?m EG, Larsson A, Ramstr?m I, Ramstr?m S, Lindahl TL
The Journal of biological chemistry283(27)18493-504(2008 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

increased thrombin activity 6 to 24 hours after germinal matrix hemorrhage, and PAR-1, PAR-4, inhibition normalized cyclooxygenase-2, and mammalian target of rapamycin by 72 hours.
Title: Protease-activated receptor 1 and 4 signal inhibition reduces preterm neonatal hemorrhagic brain injury.

activation of PAR4 upregulates the expression of CGRP mRNA and protein levels in dorsal root ganglion neurons
Title: Protease-activated receptor 4 activation increases the expression of calcitonin gene-related peptide mRNA and protein in dorsal root ganglion neurons.

PAR1 and PAR4 are functionally expressed in large myelinated fibre neurons, and are also expressed in small nociceptors of the peptidergic subclass, where they are able to potentiate TRPV1 activity.
Title: Protease activated receptors 1 and 4 sensitize TRPV1 in nociceptive neurones.

in normal rat knee joints, PAR4 activation increases joint primary afferent activity in response to mechanical stimuli. This PAR4-induced sensitization is TRPV1-independent but involves B2 receptor activation, suggesting a role for kinins in this process
Title: Proteinase-activated receptor-4 (PAR4) activation leads to sensitization of rat joint primary afferents via a bradykinin B2 receptor-dependent mechanism.

PAR 4 is regulated at the transcriptional level in a rat model of lipopolysaccharide induced acute lung injury and probably has a distinct role.
Title: Differential expression, time course and distribution of four PARs in rats with endotoxin-induced acute lung injury.

The following F2rl3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F2rl3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10706	NM_053808.1 Latest version!	Rattus norvegicus F2R like thrombin or trypsin receptor 3 (F2rl3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

F2rl3 ( NM_053808.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446260.1 Rattus norvegicus F2R like thrombin or trypsin receptor 3 (F2rl3), mRNA.

Price & Availability↑

CloneID	ORa10706
Clone ID Related Accession (Same CDS sequence)	NM_053808.1
Accession Version	NM_053808.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	proteinase-activated receptor 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF269246.2. ##Evidence-Data-START## Transcript exon combination :: AF269246.2, AF310216.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906347 [ECO:0000348] ##Evidence-Data-END##

ATGCTCGGGT TCAGCATCAG CCCGGCGGAA TGCCAGACGC CCAGCATCTA CGACGATGTA 
GAGAGTACCA GGGAAGGCCA AGAAGCCTCT CTGCGTCCCA CAGTAGAACT TAATGAGTCG 
AAGTCCCCAG ACAAGCCTAA TCCACGAGGC TTCCCGGGCA AGCCCTGTGC CAACAACAGT 
GACACGCTGG AGCTCCCAGC CAGCTCTGAA GCACTGCTTC TGGGGTGGGT CCCCACGAGG 
CTGGTACCTG CCATCTATGG GCTAGTGGTG GTCGTGGGGC TGCCTGCCAA TGGGCTAGCG 
CTGTGGGTGC TGGCCACAAG GGTCCCACGC TTGCCATCCA CCATTCTGCT CATGAACCTG 
GCAGTGGCTG ACTTGCTACT GGCCCTGGTG CTCCCACCAC GACTGGTTTA TCACCTGCGT 
GGCCAGCGCT GGCCGTTTGG GGAGGCTGCC TGCCGGGTGG CCACAGCTGC CCTCTATGGC 
CACATGTATG GTTCAGTGTT GCTTCTGGCC GCAGTCAGCC TGGACAGATA CCTGGCCCTG 
GTGCATCCTT TGCGGGCCCG GGCATTGCGT GGTCAACGCC TCACCACCAT ACTCTGCTTG 
GTGGCCTGGC TCTCTGCAGC CACTCTAGTC TTGCCACTCA CGTTTCATCG GCAGACCTTC 
CTATTGGCTG GCTCCGATCG CATGCTGTGT CATGATGCGC TGCCATTGGC GGAGCAGACC 
TCCCATTGGA GACCGGCCTT CATCTGCCTG GCTGTCCTGG GCTGCTTTGT GCCACTGCTG 
GCCATGGTCC TGTGCTATGG GGCCACCCTT CGTGCACTGG CAGCCAATGG TCAGCGGTAC 
AGCCATGCAG TGAGACTGAC AGCCCTGGTC CTGTTCTCAG CAGTGGCCGC TTTCACACCT 
AGCAATGTGC TTCTGGTGCT GCACTATTCA AACCCAAGCC CTGAGGCCTG GGGCAATCTC 
TATGGAGCCT ATGTTCCCAG CCTGGCACTC AGCACCCTTA ACAGCTGCGT AGACCCTTTC 
ATCTACTACT ATGTGTCCCA CGAGTTCAGG GAGAAGGTAC GGGCTATGTT GTGTCGCCAG 
CTGAAGGCCA GCAGCTCCTC TCAGGCCTCC AGGGAGGCTG GAAGCCGAGG GACTGCCATT 
TGCTCCTCTA CACTTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446260.1
CDS	1..1161
Translation	MLGFSISPAECQTPSIYDDVESTREGQEASLRPTVELNESKSPDKPNPRGFPGKPCANNSDTLELPASSEALLLGWVPTRLVPAIYGLVVVVGLPANGLALWVLATRVPRLPSTILLMNLAVADLLLALVLPPRLVYHLRGQRWPFGEAACRVATAALYGHMYGSVLLLAAVSLDRYLALVHPLRARALRGQRLTTILCLVAWLSAATLVLPLTFHRQTFLLAGSDRMLCHDALPLAEQTSHWRPAFICLAVLGCFVPLLAMVLCYGATLRALAANGQRYSHAVRLTALVLFSAVAAFTPSNVLLVLHYSNPSPEAWGNLYGAYVPSLALSTLNSCVDPFIYYYVSHEFREKVRAMLCRQLKASSSSQASREAGSRGTAICSSTLL

Target ORF information:

RefSeq Version	NM_053808.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus F2R like thrombin or trypsin receptor 3 (F2rl3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053808.1

ORF Insert Sequence:

ATGCTCGGGT TCAGCATCAG CCCGGCGGAA TGCCAGACGC CCAGCATCTA CGACGATGTA 
GAGAGTACCA GGGAAGGCCA AGAAGCCTCT CTGCGTCCCA CAGTAGAACT TAATGAGTCG 
AAGTCCCCAG ACAAGCCTAA TCCACGAGGC TTCCCGGGCA AGCCCTGTGC CAACAACAGT 
GACACGCTGG AGCTCCCAGC CAGCTCTGAA GCACTGCTTC TGGGGTGGGT CCCCACGAGG 
CTGGTACCTG CCATCTATGG GCTAGTGGTG GTCGTGGGGC TGCCTGCCAA TGGGCTAGCG 
CTGTGGGTGC TGGCCACAAG GGTCCCACGC TTGCCATCCA CCATTCTGCT CATGAACCTG 
GCAGTGGCTG ACTTGCTACT GGCCCTGGTG CTCCCACCAC GACTGGTTTA TCACCTGCGT 
GGCCAGCGCT GGCCGTTTGG GGAGGCTGCC TGCCGGGTGG CCACAGCTGC CCTCTATGGC 
CACATGTATG GTTCAGTGTT GCTTCTGGCC GCAGTCAGCC TGGACAGATA CCTGGCCCTG 
GTGCATCCTT TGCGGGCCCG GGCATTGCGT GGTCAACGCC TCACCACCAT ACTCTGCTTG 
GTGGCCTGGC TCTCTGCAGC CACTCTAGTC TTGCCACTCA CGTTTCATCG GCAGACCTTC 
CTATTGGCTG GCTCCGATCG CATGCTGTGT CATGATGCGC TGCCATTGGC GGAGCAGACC 
TCCCATTGGA GACCGGCCTT CATCTGCCTG GCTGTCCTGG GCTGCTTTGT GCCACTGCTG 
GCCATGGTCC TGTGCTATGG GGCCACCCTT CGTGCACTGG CAGCCAATGG TCAGCGGTAC 
AGCCATGCAG TGAGACTGAC AGCCCTGGTC CTGTTCTCAG CAGTGGCCGC TTTCACACCT 
AGCAATGTGC TTCTGGTGCT GCACTATTCA AACCCAAGCC CTGAGGCCTG GGGCAATCTC 
TATGGAGCCT ATGTTCCCAG CCTGGCACTC AGCACCCTTA ACAGCTGCGT AGACCCTTTC 
ATCTACTACT ATGTGTCCCA CGAGTTCAGG GAGAAGGTAC GGGCTATGTT GTGTCGCCAG 
CTGAAGGCCA GCAGCTCCTC TCAGGCCTCC AGGGAGGCTG GAAGCCGAGG GACTGCCATT 
TGCTCCTCTA CACTTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Protease-activated receptor 1 and 4 signal inhibition reduces preterm neonatal hemorrhagic brain injury.
Stroke46(6)1710-3(2015 Jun)
Lekic T,Klebe D,McBride DW,Manaenko A,Rolland WB,Flores JJ,Altay O,Tang J,Zhang JH

Protease-activated receptor 4 activation increases the expression of calcitonin gene-related peptide mRNA and protein in dorsal root ganglion neurons.
Journal of neuroscience research91(12)1551-62(2013 Dec)
Wang Z,Chen D,Zhang Z,Zhang R,An S,Yu L

Protease activated receptors 1 and 4 sensitize TRPV1 in nociceptive neurones.
Molecular pain661(2010 Sep)
Vellani V,Kinsey AM,Prandini M,Hechtfischer SC,Reeh P,Magherini PC,Giacomoni C,McNaughton PA

Proteinase-activated receptor-4 (PAR4) activation leads to sensitization of rat joint primary afferents via a bradykinin B2 receptor-dependent mechanism.
Journal of neurophysiology103(1)155-63(2010 Jan)
Russell FA,Veldhoen VE,Tchitchkan D,McDougall JJ

The ATP-gated P2X1 receptor plays a pivotal role in activation of aspirin-treated platelets by thrombin and epinephrine.
The Journal of biological chemistry283(27)18493-504(2008 Jul)
Greneg?rd M,Vretenbrant-Oberg K,Nylander M,D?silets S,Lindstr?m EG,Larsson A,Ramstr?m I,Ramstr?m S,Lindahl TL

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F2rl3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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