Sec14l2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sec14l2
Entrez Gene ID	116486
Full Name	SEC14-like lipid binding 2
Synonyms	Spf
General protein information	Preferred Names SEC14-like lipid binding 2 Names SEC14-like protein 2 TAP alpha-tocopherol-associated protein squalene transfer protein supernatant protein factor
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14q21
Summary	increases the activity of squalene epoxidase, which catalyzes the conversion of squalene to squalene 2,3-epoxide in cholesterol biosynthesis; may act as a cytosolic squalene transfer protein [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006251154.3	XP_006251216.1	SEC14-like protein 2 isoform X1
NM_053801.2	NP_446253.2	SEC14-like protein 2

Pathways from BioSystems

Homologies

Neurospora crassa	NCU02263	XP_959907.1
Homo sapiens (human)	SEC14L2	NP_036561.1
Rattus norvegicus (Norway rat)	Sec14l2	NP_446253.2
Danio rerio (zebrafish)	si:ch211-89f7.1	NP_001093463.1
Caenorhabditis elegans (roundworm)	ctg-1	NP_491993.1
Xenopus tropicalis (tropical clawed frog)	sec14l3	NP_001120265.1
Pan troglodytes (chimpanzee)	SEC14L2	XP_515071.1
Saccharomyces cerevisiae (baker's yeast)	SEC14	NP_013796.1
Magnaporthe oryzae (rice blast fungus)	MGG_00905	XP_003718055.1
Canis lupus familiaris (dog)	SEC14L2	XP_534734.3
Bos taurus (cattle)	SEC14L2	NP_808812.2
Eremothecium gossypii	AGOS_ABR020W	NP_982966.2
	LOC718031	XP_002808012.1
Mus musculus (house mouse)	Sec14l2	NP_653103.1
Kluyveromyces lactis	KLLA0B06479g	XP_451823.1
Schizosaccharomyces pombe (fission yeast)	spo20	NP_593003.1

Gene Ontology
Bibliography

Related articles in PubMed

SEC14-like protein 1 interacts with cholinergic transporters.
Ribeiro FM, Ferreira LT, Marion S, Fontes S, Gomez M, Ferguson SS, Prado MA, Prado VF
Neurochemistry international50(2)356-64(2007 Jan)

Supernatant protein factor requires phosphorylation and interaction with Golgi to stimulate cholesterol synthesis in hepatoma cells.
Mokashi V, Porter TD
Archives of biochemistry and biophysics435(1)175-81(2005 Mar)

Supernatant protein factor stimulates HMG-CoA reductase in cell culture and in vitro.
Mokashi V, Singh DK, Porter TD
Archives of biochemistry and biophysics433(2)474-80(2005 Jan)

Tocopherol-associated protein is a ligand-dependent transcriptional activator.
Yamauchi J, Iwamoto T, Kida S, Masushige S, Yamada K, Esashi T
Biochemical and biophysical research communications285(2)295-9(2001 Jul)

Supernatant protein factor, which stimulates the conversion of squalene to lanosterol, is a cytosolic squalene transfer protein and enhances cholesterol biosynthesis.
Shibata N, Arita M, Misaki Y, Dohmae N, Takio K, Ono T, Inoue K, Arai H
Proceedings of the National Academy of Sciences of the United States of America98(5)2244-9(2001 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

overexpression of SEC14L1 modestly decreases high affinity choline transport activity
Title: SEC14-like protein 1 interacts with cholinergic transporters.

The activation of SPF by PKA suggests that cholesterol synthesis can be rapidly modulated in response to external stimuli by changes in cAMP levels, and that this regulation is dependent on an as yet undefined interaction with Golgi.
Title: Supernatant protein factor requires phosphorylation and interaction with Golgi to stimulate cholesterol synthesis in hepatoma cells.

Sec14l2 stimulates HMG-CoA reductase in hepatoma cell cultures.
Title: Supernatant protein factor stimulates HMG-CoA reductase in cell culture and in vitro.

The following Sec14l2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sec14l2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10681	NM_053801.2 Latest version!	Rattus norvegicus SEC14-like lipid binding 2 (Sec14l2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
ORa36196	XM_006251154.3 Latest version!	Rattus norvegicus SEC14-like lipid binding 2 (Sec14l2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sec14l2 ( NM_053801.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446253.2 Rattus norvegicus SEC14-like lipid binding 2 (Sec14l2), mRNA.

Price & Availability↑

CloneID	ORa10681
Clone ID Related Accession (Same CDS sequence)	NM_053801.2
Accession Version	NM_053801.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Rattus norvegicus(Norway rat)
Product	SEC14-like protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC089785.1. On Feb 20, 2005 this sequence version replaced NM_053801.1. ##Evidence-Data-START## Transcript exon combination :: BC089785.1, AF309558.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAGCGGGA GAGTCGGTGA CCTGAGCCCC AAACAGGAGG AGGCACTGGC CAAGTTTCGA 
GAAAATGTCC AGGACGTGCT GCCTGCCCTG CCCAATCCAG ATGACTACTT TCTCCTTCGA 
TGGCTCCGAG CCCGAAGCTT TGACCTGCAG AAGTCGGAGG CCATGCTCCG GAAGCATGTG 
GAATTCCGGA AGCAAAAGGA CATTGACAAA ATCATCAGCT GGCAGCCACC AGAGGTGATC 
CAACAGTATC TGTCAGGCGG CCGGTGTGGG TATGACCTGG ACGGCTGCCC TGTCTGGTAT 
GACATCATCG GCCCACTGGA TGCCAAGGGT CTGCTGTTCT CCGCCTCCAA GCAAGACCTG 
CTCAGGACCA AGATGAGAGA TTGTGAGCTG CTTCTGCAGG AGTGCACCCA TCAGACCGCA 
AAGCTAGGGA AGAAGATAGA GACCATCACC ATGATTTACG ACTGTGAGGG ACTCGGCCTC 
AAGCACCTCT GGAAACCTGC AGTGGAGGCC TATGGAGAGT TTCTCACCAT GTTTGAGGAG 
AACTATCCTG AAACGCTGAA GCGGCTGTTC GTCGTTAAAG CCCCCAAGCT GTTTCCAGTG 
GCCTACAACC TCATCAAGCC CTTCCTGAGT GAGGACACTA GGAAGAAGAT CATGGTTCTG 
GGAGCAAACT GGAAGGAGGT TTTACTCAAA CACATCAGCC CCGACCAGTT GCCTGTGGAG 
TATGGAGGCA CCATGACGGA CCCTGATGGA AACCCCAAGT GTAAATCCAA GATCAACTAT 
GGGGGTGACA TCCCCAAGCA GTATTACGTG CGAGACCAGG TGAAGCAGCA GTATGAACAC 
AGTGTGCAGA TCTCCCGAGG CTCCTCCCAC CAAGTAGAGT ATGAGATTCT TTTCCCCGGC 
TGCGTCCTCA GGTGGCAGTT TATGTCGGAG GGATCAGACG TGGGTTTTGG GATTTTCCTG 
AAGACCAAGA TGGGGGAACG GCAGCGGGCA GGGGAGATGA CGGAGGTGCT GCCTAACCAG 
AGATACAATT CCCACATGGT CCCTGAGGAT GGGACCCTCA CCTGCAGTGA GCCAGGCATC 
TATGTCCTAC GGTTTGACAA CACCTACAGC TTCATCCATG CCAAGAAAGT CAGTTTCACT 
GTGGAGGTCC TGCTTCCAGA CAAAGCAGCA GAAGAGAAGT TGAATCAGCA GGGGACAGTC 
ACCCCCAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446253.2
CDS	49..1260
Translation	MSGRVGDLSPKQEEALAKFRENVQDVLPALPNPDDYFLLRWLRARSFDLQKSEAMLRKHVEFRKQKDIDKIISWQPPEVIQQYLSGGRCGYDLDGCPVWYDIIGPLDAKGLLFSASKQDLLRTKMRDCELLLQECTHQTAKLGKKIETITMIYDCEGLGLKHLWKPAVEAYGEFLTMFEENYPETLKRLFVVKAPKLFPVAYNLIKPFLSEDTRKKIMVLGANWKEVLLKHISPDQLPVEYGGTMTDPDGNPKCKSKINYGGDIPKQYYVRDQVKQQYEHSVQISRGSSHQVEYEILFPGCVLRWQFMSEGSDVGFGIFLKTKMGERQRAGEMTEVLPNQRYNSHMVPEDGTLTCSEPGIYVLRFDNTYSFIHAKKVSFTVEVLLPDKAAEEKLNQQGTVTPK

Target ORF information:

RefSeq Version	NM_053801.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SEC14-like lipid binding 2 (Sec14l2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053801.2

ORF Insert Sequence:

ATGAGCGGGA GAGTCGGTGA CCTGAGCCCC AAACAGGAGG AGGCACTGGC CAAGTTTCGA 
GAAAATGTCC AGGACGTGCT GCCTGCCCTG CCCAATCCAG ATGACTACTT TCTCCTTCGA 
TGGCTCCGAG CCCGAAGCTT TGACCTGCAG AAGTCGGAGG CCATGCTCCG GAAGCATGTG 
GAATTCCGGA AGCAAAAGGA CATTGACAAA ATCATCAGCT GGCAGCCACC AGAGGTGATC 
CAACAGTATC TGTCAGGCGG CCGGTGTGGG TATGACCTGG ACGGCTGCCC TGTCTGGTAT 
GACATCATCG GCCCACTGGA TGCCAAGGGT CTGCTGTTCT CCGCCTCCAA GCAAGACCTG 
CTCAGGACCA AGATGAGAGA TTGTGAGCTG CTTCTGCAGG AGTGCACCCA TCAGACCGCA 
AAGCTAGGGA AGAAGATAGA GACCATCACC ATGATTTACG ACTGTGAGGG ACTCGGCCTC 
AAGCACCTCT GGAAACCTGC AGTGGAGGCC TATGGAGAGT TTCTCACCAT GTTTGAGGAG 
AACTATCCTG AAACGCTGAA GCGGCTGTTC GTCGTTAAAG CCCCCAAGCT GTTTCCAGTG 
GCCTACAACC TCATCAAGCC CTTCCTGAGT GAGGACACTA GGAAGAAGAT CATGGTTCTG 
GGAGCAAACT GGAAGGAGGT TTTACTCAAA CACATCAGCC CCGACCAGTT GCCTGTGGAG 
TATGGAGGCA CCATGACGGA CCCTGATGGA AACCCCAAGT GTAAATCCAA GATCAACTAT 
GGGGGTGACA TCCCCAAGCA GTATTACGTG CGAGACCAGG TGAAGCAGCA GTATGAACAC 
AGTGTGCAGA TCTCCCGAGG CTCCTCCCAC CAAGTAGAGT ATGAGATTCT TTTCCCCGGC 
TGCGTCCTCA GGTGGCAGTT TATGTCGGAG GGATCAGACG TGGGTTTTGG GATTTTCCTG 
AAGACCAAGA TGGGGGAACG GCAGCGGGCA GGGGAGATGA CGGAGGTGCT GCCTAACCAG 
AGATACAATT CCCACATGGT CCCTGAGGAT GGGACCCTCA CCTGCAGTGA GCCAGGCATC 
TATGTCCTAC GGTTTGACAA CACCTACAGC TTCATCCATG CCAAGAAAGT CAGTTTCACT 
GTGGAGGTCC TGCTTCCAGA CAAAGCAGCA GAAGAGAAGT TGAATCAGCA GGGGACAGTC 
ACCCCCAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec14l2 ( XM_006251154.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006251216.1 Rattus norvegicus SEC14-like lipid binding 2 (Sec14l2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa36196
Clone ID Related Accession (Same CDS sequence)	XM_006251154.3
Accession Version	XM_006251154.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	SEC14-like protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006251154.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCGGA AGCATGTGGA ATTCCGGAAG CAAAAGGACA TTGACAAAAT CATCAGCTGG 
CAGCCACCAG AGGTGATCCA ACAGTATCTG TCAGGCGGCC GGTGTGGGTA TGACCTGGAC 
GGCTGCCCTG TCTGGTATGA CATCATCGGC CCACTGGATG CCAAGGGTCT GCTGTTCTCC 
GCCTCCAAGC AAGACCTGCT CAGGACCAAG ATGAGAGATT GTGAGCTGCT TCTGCAGGAG 
TGCACCCATC AGACCGCAAA GCTAGGGAAG AAGATAGAGA CCATCACCAT GATTTACGAC 
TGTGAGGGAC TCGGCCTCAA GCACCTCTGG AAACCTGCAG TGGAGGCCTA TGGAGAGTTT 
CTCACCATGT TTGAGGAGAA CTATCCTGAA ACGCTGAAGC GGCTGTTCGT CGTTAAAGCC 
CCCAAGCTGT TTCCAGTGGC CTACAACCTC ATCAAGCCCT TCCTGAGTGA GGACACTAGG 
AAGAAGATCA TGGTTCTGGG AGCAAACTGG AAGGAGGTTT TACTCAAACA CATCAGCCCC 
GACCAGTTGC CTGTGGAGTA TGGAGGCACC ATGACGGACC CTGATGGAAA CCCCAAGTGT 
AAATCCAAGA TCAACTATGG GGGTGACATC CCCAAGCAGT ATTACGTGCG AGACCAGGTG 
AAGCAGCAGT ATGAACACAG TGTGCAGATC TCCCGAGGCT CCTCCCACCA AGTAGAGTAT 
GAGATTCTTT TCCCCGGCTG CGTCCTCAGG TGGCAGTTTA TGTCGGAGGG ATCAGACGTG 
GGTTTTGGGA TTTTCCTGAA GACCAAGATG GGGGAACGGC AGCGGGCAGG GGAGATGACG 
GAGGTGCTGC CTAACCAGAG ATACAATTCC CACATGGTCC CTGAGGATGG GACCCTCACC 
TGCAGTGAGC CAGGCATCTA TGTCCTACGG TTTGACAACA CCTACAGCTT CATCCATGCC 
AAGAAAGTCA GTTTCACTGT GGAGGTCCTG CTTCCAGACA AAGCAGCAGA AGAGAAGTTG 
AATCAGCAGG GGACAGTCAC CCCCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006251216.1
CDS	419..1468
Translation	MLRKHVEFRKQKDIDKIISWQPPEVIQQYLSGGRCGYDLDGCPVWYDIIGPLDAKGLLFSASKQDLLRTKMRDCELLLQECTHQTAKLGKKIETITMIYDCEGLGLKHLWKPAVEAYGEFLTMFEENYPETLKRLFVVKAPKLFPVAYNLIKPFLSEDTRKKIMVLGANWKEVLLKHISPDQLPVEYGGTMTDPDGNPKCKSKINYGGDIPKQYYVRDQVKQQYEHSVQISRGSSHQVEYEILFPGCVLRWQFMSEGSDVGFGIFLKTKMGERQRAGEMTEVLPNQRYNSHMVPEDGTLTCSEPGIYVLRFDNTYSFIHAKKVSFTVEVLLPDKAAEEKLNQQGTVTPK

Target ORF information:

RefSeq Version	XM_006251154.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SEC14-like lipid binding 2 (Sec14l2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006251154.3

ORF Insert Sequence:

ATGCTCCGGA AGCATGTGGA ATTCCGGAAG CAAAAGGACA TTGACAAAAT CATCAGCTGG 
CAGCCACCAG AGGTGATCCA ACAGTATCTG TCAGGCGGCC GGTGTGGGTA TGACCTGGAC 
GGCTGCCCTG TCTGGTATGA CATCATCGGC CCACTGGATG CCAAGGGTCT GCTGTTCTCC 
GCCTCCAAGC AAGACCTGCT CAGGACCAAG ATGAGAGATT GTGAGCTGCT TCTGCAGGAG 
TGCACCCATC AGACCGCAAA GCTAGGGAAG AAGATAGAGA CCATCACCAT GATTTACGAC 
TGTGAGGGAC TCGGCCTCAA GCACCTCTGG AAACCTGCAG TGGAGGCCTA TGGAGAGTTT 
CTCACCATGT TTGAGGAGAA CTATCCTGAA ACGCTGAAGC GGCTGTTCGT CGTTAAAGCC 
CCCAAGCTGT TTCCAGTGGC CTACAACCTC ATCAAGCCCT TCCTGAGTGA GGACACTAGG 
AAGAAGATCA TGGTTCTGGG AGCAAACTGG AAGGAGGTTT TACTCAAACA CATCAGCCCC 
GACCAGTTGC CTGTGGAGTA TGGAGGCACC ATGACGGACC CTGATGGAAA CCCCAAGTGT 
AAATCCAAGA TCAACTATGG GGGTGACATC CCCAAGCAGT ATTACGTGCG AGACCAGGTG 
AAGCAGCAGT ATGAACACAG TGTGCAGATC TCCCGAGGCT CCTCCCACCA AGTAGAGTAT 
GAGATTCTTT TCCCCGGCTG CGTCCTCAGG TGGCAGTTTA TGTCGGAGGG ATCAGACGTG 
GGTTTTGGGA TTTTCCTGAA GACCAAGATG GGGGAACGGC AGCGGGCAGG GGAGATGACG 
GAGGTGCTGC CTAACCAGAG ATACAATTCC CACATGGTCC CTGAGGATGG GACCCTCACC 
TGCAGTGAGC CAGGCATCTA TGTCCTACGG TTTGACAACA CCTACAGCTT CATCCATGCC 
AAGAAAGTCA GTTTCACTGT GGAGGTCCTG CTTCCAGACA AAGCAGCAGA AGAGAAGTTG 
AATCAGCAGG GGACAGTCAC CCCCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SEC14-like protein 1 interacts with cholinergic transporters.
Neurochemistry international50(2)356-64(2007 Jan)
Ribeiro FM,Ferreira LT,Marion S,Fontes S,Gomez M,Ferguson SS,Prado MA,Prado VF

Supernatant protein factor requires phosphorylation and interaction with Golgi to stimulate cholesterol synthesis in hepatoma cells.
Archives of biochemistry and biophysics435(1)175-81(2005 Mar)
Mokashi V,Porter TD

Supernatant protein factor stimulates HMG-CoA reductase in cell culture and in vitro.
Archives of biochemistry and biophysics433(2)474-80(2005 Jan)
Mokashi V,Singh DK,Porter TD

Tocopherol-associated protein is a ligand-dependent transcriptional activator.
Biochemical and biophysical research communications285(2)295-9(2001 Jul)
Yamauchi J,Iwamoto T,Kida S,Masushige S,Yamada K,Esashi T

Supernatant protein factor, which stimulates the conversion of squalene to lanosterol, is a cytosolic squalene transfer protein and enhances cholesterol biosynthesis.
Proceedings of the National Academy of Sciences of the United States of America98(5)2244-9(2001 Feb)
Shibata N,Arita M,Misaki Y,Dohmae N,Takio K,Ono T,Inoue K,Arai H