Atp6v0a2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Atp6v0a2
Entrez Gene ID	116455
Full Name	ATPase H+ transporting V0 subunit a2
Synonyms	Cc1-3,J6b7,Tj6
General protein information	Preferred Names ATPase H+ transporting V0 subunit a2 Names V-type proton ATPase 116 kDa subunit a ATPase, H+ transporting, lysosomal V0 subunit A2 T-cell expressing clone j6
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q15
Summary	tumor associated antigen; may play a role in immune response [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053775.3	NP_446227.2	V-type proton ATPase 116 kDa subunit a

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ATP6V0A2	XP_543370.2
Bos taurus (cattle)	ATP6V0A2	NP_788810.1
Rattus norvegicus (Norway rat)	Atp6v0a2	NP_446227.2
Pan troglodytes (chimpanzee)	ATP6V0A2	XP_003314058.1
Homo sapiens (human)	ATP6V0A2	NP_036595.2
Mus musculus (house mouse)	Atp6v0a2	NP_035726.2
Gallus gallus (chicken)	ATP6V0A2	NP_990054.1
Danio rerio (zebrafish)	atp6v0a2a	XP_708125.5
Xenopus tropicalis (tropical clawed frog)	atp6v0a2	NP_001016223.1
Danio rerio (zebrafish)	atp6v0a2b	NP_001116219.1

Gene Ontology
Bibliography

Related articles in PubMed

The vacuolar-ATPase complex and assembly factors, TMEM199 and CCDC115, control HIF1? prolyl hydroxylation by regulating cellular iron levels.
Miles AL, Burr SP, Grice GL, Nathan JA
eLife6(2017 03)

Vacuolar H(+)-ATPase subunits Voa1 and Voa2 cooperatively regulate secretory vesicle acidification, transmitter uptake, and storage.
Saw NM, Kang SY, Parsaud L, Han GA, Jiang T, Grzegorczyk K, Surkont M, Sun-Wada GH, Wada Y, Li L, Sugita S
Molecular biology of the cell22(18)3394-409(2011 Sep)

Direct recruitment of H+-ATPase from lysosomes for phagosomal acidification.
Sun-Wada GH, Tabata H, Kawamura N, Aoyama M, Wada Y
Journal of cell science122(Pt 14)2504-13(2009 Jul)

Integral and associated lysosomal membrane proteins.
Schr?der B, Wrocklage C, Pan C, J?ger R, K?sters B, Sch?fer H, Els?sser HP, Mann M, Hasilik A
Traffic (Copenhagen, Denmark)8(12)1676-86(2007 Dec)

Alternative splicing controls neuronal expression of v-ATPase subunit a1 and sorting to nerve terminals.
Po?a-Guyon S, Amar M, Fossier P, Morel N
The Journal of biological chemistry281(25)17164-72(2006 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Voa1 and Voa2 cooperatively regulate the acidification and transmitter uptake/storage of dense-core vesicles, whereas they might not be as critical for exocytosis as recently proposed.
Title: Vacuolar H(+)-ATPase subunits Voa1 and Voa2 cooperatively regulate secretory vesicle acidification, transmitter uptake, and storage.

The following Atp6v0a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Atp6v0a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10610	NM_053775.3 Latest version!	Rattus norvegicus ATPase H+ transporting V0 subunit a2 (Atp6v0a2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$503.30 $719.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Atp6v0a2 ( NM_053775.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446227.2 Rattus norvegicus ATPase H+ transporting V0 subunit a2 (Atp6v0a2), mRNA.

Price & Availability↑

CloneID	ORa10610
Clone ID Related Accession (Same CDS sequence)	NM_053775.3
Accession Version	NM_053775.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	V-type proton ATPase 116 kDa subunit a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ286425.1. On Aug 12, 2007 this sequence version replaced NM_053775.2. ##Evidence-Data-START## Transcript exon combination :: DQ286425.1, BC168659.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCTCCC TCTTCCGTAG CGAGAGCATG TGCCTGGCGC AGCTCTTCCT GCAGTCGGGC 
ACGGCCTACG AGTGTCTGAG CGCGCTTGGC GAGAAGGGCC TGGTGCAGTT CCGAGACCTC 
AACCAAAATG TAAGTTCTTT CCAAAGAAAA TTCGTTGGTG AGGTAAAGAG GTGTGAAGAA 
CTAGAACGAA TATTGGTGTA CCTGGTGCAG GAAATCACCA GAGCTGATAT TCCCCTGCCT 
GAGGGAGAGG CCAGTCCTCC CGCACCACCT GTTAAACATG TTCTAGAAAT GCAGGAGCAG 
CTGCAGAAGC TCGAGGTGGA GCTCAGAGAA GTCACCAAGA ACAAGGAGAA GCTGAGGAAG 
AACCTGCTGG AGCTGGTGGA GTATACCCAC ATGCTGAGGG TCACCAAGAC CTTCCTTAAA 
CGGAACGTGG AGTTTGAGCC AACTTACGAA GAGTTCCCCG CCTTAGAGAG CGACTCTCTG 
TTGGACTACA GCTGCATGCA GCGGCTGGGC GCCAAGCTGG GATTCGTTTC TGGCCTGATT 
CAGCAAGGCA AAGTGGAAGC ATTTGAAAGA ATGCTGTGGC GGGCCTGCAA AGGGTACACC 
ATTGTGACCT ATGCAGAGCT GGACGAGGCC CTGGAAGACC CTGAGACGGG TGAAGTCATA 
AAGTGGTATG TGTTCCTGAT CTCGTTTTGG GGAGAGCAGA TTGGCCACAA GGTTAAGAAG 
ATCTGTGACT GTTACCACTG CCACATCTAC CCGTATCCAA ACACGGCCGA GGAGCGCAGG 
GAGATCCAGG AGGGGCTCAA TACTCGGATC CAAGACCTTT ACACTGTGCT GCACAAAACG 
GAGGACTACC TGCGGCAAGT GCTGTGCAAG GCTGCCGAGT CCGTGTGCAG CCGTGTGATA 
CAGGTGCGGA AGATGAAAGC CATCTACCAC ATGCTCAACA TGTGCAGCTT TGACGTCACC 
AACAAGTGCC TCATAGCTGA GGTCTGGTGC CCCGAGGTGG ACCTGCCCGG CTTGCGCAGA 
GCGCTGGAGG AAGGCTCGAG AGAAAGTGGA GCTACAATCC CCTCGTTCAT GAACACAATC 
CCTACGAAAG AAACGCCGCC CACTCTGATC CGCACCAACA AATTCACTGA AGGTTTCCAG 
AACATCGTGG ATGCCTACGG TGTTGGGAGC TACAGAGAAG TGAACCCAGC TTTGTTCACC 
ATCATCACCT TCCCGTTCCT GTTTGGCGTG ATGTTTGGTG ACTTGGGGCA CGGCTTTGTC 
ATGTTCCTGT TTGCCCTCTT ACTGGTGTTG AATGAGAATC ACCCCAGACT AAGCCAGTCA 
CAGGAGATCC TTAGGATGTT CTTTGATGGC CGATATATCC TGCTGCTGAT GGGGCTGTTC 
TCCGTGTACA CCGGCCTCAT CTACAACGAC TGCTTCTCCA AGTCCCTGAA CCTCTTTGGC 
TCTGGGTGGA ACGTGTCTGC CATGTACAGT TCCAGCCACT CCCCAGAGGA GCAAAGGAAA 
ATGGTGCTTT GGAATGACAG CACCATCAGG CACAGCAGGA CGCTGCAGCT GGACCCAAAC 
ATCCCTGGAG TTTTCCGAGG CCCCTACCCT TTCGGCATCG ATCCTATTTG GAACCTGGCC 
ACAAATCGCC TCACGTTCCT CAACTCCTTC AAGATGAAGA TGTCTGTGAT TTTAGGAATT 
TTTCACATGA CCTTTGGTGT CATTCTGGGA ATATTTAACC ACTTGCACTT CAGGAAGAAG 
TTCAACATCT ACCTGGTCTC GGTCCCCGAG ATCCTGTTTA TGCTCTGCAT CTTTGGGTAC 
CTGATTTTCA TGATCATCTA CAAGTGGCTG GCGTACTCGG CAGAGACCTC CAGAGAGGCC 
CCCAGCATCC TCATCGAGTT CATTAACATG TTCCTGTTCC CGTCCAGTGA GACACACGGC 
CTCTACCCGG GGCAGGCACA CGTCCAGAAG GTGTTGTTGG CTCTCACCGT GCTGGCTGTC 
CCTGTGCTCT TCTTGGGAAA GCCGCTCTTT CTGCTGTGGC TGCACAATGG GCGCAGTTGC 
TTCGGCGTGA GCCGGAGCGG CTACACGCTC GTGAGGAAGG ACAGCGAGGA AGAGGTGTCT 
CTCCTAGGCA GCCAGGACAT AGAGGAGGGG AACAACCGCA TGGAAGAGGG CTGCCGAGAA 
ATGACCTGTG AGGAGTTTAA CTTCGGGGAG ATCCTGATGA CTCAGGCCAT CCACTCCATC 
GAGTACTGCC TTGGCTGCAT CTCCAACACT GCGTCCTACC TGAGGCTTTG GGCTCTCAGC 
CTGGCACACG CACAGCTCTC GGATGTGCTG TGGGCCATGC TGATGCGTGT AGGCCTGCGT 
GTGGACAACA CTTACGGGGT CCTGCTGCTG CTGCCCGTCA TGACTTTCTT TGCAGTTCTG 
ACCGTTTTTA TCCTTCTGGT CATGGAAGGG CTTTCTGCAT TTCTCCACGC CATCCGGCTT 
CACTGGGTAG AATTTCAGAA CAAATTCTAC GTTGGTGCAG GTACCAAATT CGTTCCTTTC 
TCCTTCAGTC TGCTGTCCTC CAAGTTCAGT AAAGATGACA GCATAGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446227.2
CDS	1..2571
Translation	MGSLFRSESMCLAQLFLQSGTAYECLSALGEKGLVQFRDLNQNVSSFQRKFVGEVKRCEELERILVYLVQEITRADIPLPEGEASPPAPPVKHVLEMQEQLQKLEVELREVTKNKEKLRKNLLELVEYTHMLRVTKTFLKRNVEFEPTYEEFPALESDSLLDYSCMQRLGAKLGFVSGLIQQGKVEAFERMLWRACKGYTIVTYAELDEALEDPETGEVIKWYVFLISFWGEQIGHKVKKICDCYHCHIYPYPNTAEERREIQEGLNTRIQDLYTVLHKTEDYLRQVLCKAAESVCSRVIQVRKMKAIYHMLNMCSFDVTNKCLIAEVWCPEVDLPGLRRALEEGSRESGATIPSFMNTIPTKETPPTLIRTNKFTEGFQNIVDAYGVGSYREVNPALFTIITFPFLFGVMFGDLGHGFVMFLFALLLVLNENHPRLSQSQEILRMFFDGRYILLLMGLFSVYTGLIYNDCFSKSLNLFGSGWNVSAMYSSSHSPEEQRKMVLWNDSTIRHSRTLQLDPNIPGVFRGPYPFGIDPIWNLATNRLTFLNSFKMKMSVILGIFHMTFGVILGIFNHLHFRKKFNIYLVSVPEILFMLCIFGYLIFMIIYKWLAYSAETSREAPSILIEFINMFLFPSSETHGLYPGQAHVQKVLLALTVLAVPVLFLGKPLFLLWLHNGRSCFGVSRSGYTLVRKDSEEEVSLLGSQDIEEGNNRMEEGCREMTCEEFNFGEILMTQAIHSIEYCLGCISNTASYLRLWALSLAHAQLSDVLWAMLMRVGLRVDNTYGVLLLLPVMTFFAVLTVFILLVMEGLSAFLHAIRLHWVEFQNKFYVGAGTKFVPFSFSLLSSKFSKDDSIA

Target ORF information:

RefSeq Version	NM_053775.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATPase H+ transporting V0 subunit a2 (Atp6v0a2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053775.3

ORF Insert Sequence:

ATGGGCTCCC TCTTCCGTAG CGAGAGCATG TGCCTGGCGC AGCTCTTCCT GCAGTCGGGC 
ACGGCCTACG AGTGTCTGAG CGCGCTTGGC GAGAAGGGCC TGGTGCAGTT CCGAGACCTC 
AACCAAAATG TAAGTTCTTT CCAAAGAAAA TTCGTTGGTG AGGTAAAGAG GTGTGAAGAA 
CTAGAACGAA TATTGGTGTA CCTGGTGCAG GAAATCACCA GAGCTGATAT TCCCCTGCCT 
GAGGGAGAGG CCAGTCCTCC CGCACCACCT GTTAAACATG TTCTAGAAAT GCAGGAGCAG 
CTGCAGAAGC TCGAGGTGGA GCTCAGAGAA GTCACCAAGA ACAAGGAGAA GCTGAGGAAG 
AACCTGCTGG AGCTGGTGGA GTATACCCAC ATGCTGAGGG TCACCAAGAC CTTCCTTAAA 
CGGAACGTGG AGTTTGAGCC AACTTACGAA GAGTTCCCCG CCTTAGAGAG CGACTCTCTG 
TTGGACTACA GCTGCATGCA GCGGCTGGGC GCCAAGCTGG GATTCGTTTC TGGCCTGATT 
CAGCAAGGCA AAGTGGAAGC ATTTGAAAGA ATGCTGTGGC GGGCCTGCAA AGGGTACACC 
ATTGTGACCT ATGCAGAGCT GGACGAGGCC CTGGAAGACC CTGAGACGGG TGAAGTCATA 
AAGTGGTATG TGTTCCTGAT CTCGTTTTGG GGAGAGCAGA TTGGCCACAA GGTTAAGAAG 
ATCTGTGACT GTTACCACTG CCACATCTAC CCGTATCCAA ACACGGCCGA GGAGCGCAGG 
GAGATCCAGG AGGGGCTCAA TACTCGGATC CAAGACCTTT ACACTGTGCT GCACAAAACG 
GAGGACTACC TGCGGCAAGT GCTGTGCAAG GCTGCCGAGT CCGTGTGCAG CCGTGTGATA 
CAGGTGCGGA AGATGAAAGC CATCTACCAC ATGCTCAACA TGTGCAGCTT TGACGTCACC 
AACAAGTGCC TCATAGCTGA GGTCTGGTGC CCCGAGGTGG ACCTGCCCGG CTTGCGCAGA 
GCGCTGGAGG AAGGCTCGAG AGAAAGTGGA GCTACAATCC CCTCGTTCAT GAACACAATC 
CCTACGAAAG AAACGCCGCC CACTCTGATC CGCACCAACA AATTCACTGA AGGTTTCCAG 
AACATCGTGG ATGCCTACGG TGTTGGGAGC TACAGAGAAG TGAACCCAGC TTTGTTCACC 
ATCATCACCT TCCCGTTCCT GTTTGGCGTG ATGTTTGGTG ACTTGGGGCA CGGCTTTGTC 
ATGTTCCTGT TTGCCCTCTT ACTGGTGTTG AATGAGAATC ACCCCAGACT AAGCCAGTCA 
CAGGAGATCC TTAGGATGTT CTTTGATGGC CGATATATCC TGCTGCTGAT GGGGCTGTTC 
TCCGTGTACA CCGGCCTCAT CTACAACGAC TGCTTCTCCA AGTCCCTGAA CCTCTTTGGC 
TCTGGGTGGA ACGTGTCTGC CATGTACAGT TCCAGCCACT CCCCAGAGGA GCAAAGGAAA 
ATGGTGCTTT GGAATGACAG CACCATCAGG CACAGCAGGA CGCTGCAGCT GGACCCAAAC 
ATCCCTGGAG TTTTCCGAGG CCCCTACCCT TTCGGCATCG ATCCTATTTG GAACCTGGCC 
ACAAATCGCC TCACGTTCCT CAACTCCTTC AAGATGAAGA TGTCTGTGAT TTTAGGAATT 
TTTCACATGA CCTTTGGTGT CATTCTGGGA ATATTTAACC ACTTGCACTT CAGGAAGAAG 
TTCAACATCT ACCTGGTCTC GGTCCCCGAG ATCCTGTTTA TGCTCTGCAT CTTTGGGTAC 
CTGATTTTCA TGATCATCTA CAAGTGGCTG GCGTACTCGG CAGAGACCTC CAGAGAGGCC 
CCCAGCATCC TCATCGAGTT CATTAACATG TTCCTGTTCC CGTCCAGTGA GACACACGGC 
CTCTACCCGG GGCAGGCACA CGTCCAGAAG GTGTTGTTGG CTCTCACCGT GCTGGCTGTC 
CCTGTGCTCT TCTTGGGAAA GCCGCTCTTT CTGCTGTGGC TGCACAATGG GCGCAGTTGC 
TTCGGCGTGA GCCGGAGCGG CTACACGCTC GTGAGGAAGG ACAGCGAGGA AGAGGTGTCT 
CTCCTAGGCA GCCAGGACAT AGAGGAGGGG AACAACCGCA TGGAAGAGGG CTGCCGAGAA 
ATGACCTGTG AGGAGTTTAA CTTCGGGGAG ATCCTGATGA CTCAGGCCAT CCACTCCATC 
GAGTACTGCC TTGGCTGCAT CTCCAACACT GCGTCCTACC TGAGGCTTTG GGCTCTCAGC 
CTGGCACACG CACAGCTCTC GGATGTGCTG TGGGCCATGC TGATGCGTGT AGGCCTGCGT 
GTGGACAACA CTTACGGGGT CCTGCTGCTG CTGCCCGTCA TGACTTTCTT TGCAGTTCTG 
ACCGTTTTTA TCCTTCTGGT CATGGAAGGG CTTTCTGCAT TTCTCCACGC CATCCGGCTT 
CACTGGGTAG AATTTCAGAA CAAATTCTAC GTTGGTGCAG GTACCAAATT CGTTCCTTTC 
TCCTTCAGTC TGCTGTCCTC CAAGTTCAGT AAAGATGACA GCATAGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The vacuolar-ATPase complex and assembly factors, TMEM199 and CCDC115, control HIF1? prolyl hydroxylation by regulating cellular iron levels.
eLife6(2017 03)
Miles AL,Burr SP,Grice GL,Nathan JA

Vacuolar H(+)-ATPase subunits Voa1 and Voa2 cooperatively regulate secretory vesicle acidification, transmitter uptake, and storage.
Molecular biology of the cell22(18)3394-409(2011 Sep)
Saw NM,Kang SY,Parsaud L,Han GA,Jiang T,Grzegorczyk K,Surkont M,Sun-Wada GH,Wada Y,Li L,Sugita S

Direct recruitment of H+-ATPase from lysosomes for phagosomal acidification.
Journal of cell science122(Pt 14)2504-13(2009 Jul)
Sun-Wada GH,Tabata H,Kawamura N,Aoyama M,Wada Y

Integral and associated lysosomal membrane proteins.
Traffic (Copenhagen, Denmark)8(12)1676-86(2007 Dec)
Schr?der B,Wrocklage C,Pan C,J?ger R,K?sters B,Sch?fer H,Els?sser HP,Mann M,Hasilik A

Alternative splicing controls neuronal expression of v-ATPase subunit a1 and sorting to nerve terminals.
The Journal of biological chemistry281(25)17164-72(2006 Jun)
Po?a-Guyon S,Amar M,Fossier P,Morel N

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Atp6v0a2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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