Tjp2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tjp2
Entrez Gene ID	115769
Full Name	tight junction protein 2
Synonyms	ZO-2
General protein information	Preferred Names tight junction protein 2 Names tight junction protein ZO-2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q51
Summary	a tight junction protein that may be involved in carotid neointimal formation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006231175.3	XP_006231237.1	tight junction protein ZO-2 isoform X1
XM_006231176.3	XP_006231238.1	tight junction protein ZO-2 isoform X2
NM_053773.1	NP_446225.1	tight junction protein ZO-2
XM_006231181.3	XP_006231243.1	tight junction protein ZO-2 isoform X5
XM_006231182.3	XP_006231244.1	tight junction protein ZO-2 isoform X6
XM_008760302.2	XP_008758524.1	tight junction protein ZO-2 isoform X5
XM_006231177.3	XP_006231239.1	tight junction protein ZO-2 isoform X3
XM_006231178.3	XP_006231240.1	tight junction protein ZO-2 isoform X4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TJP2	XP_003312175.1
Danio rerio (zebrafish)	tjp2a	NP_001188499.1
Xenopus tropicalis (tropical clawed frog)	tjp2	XP_004910833.1
	LOC699586	XP_002808149.1
Canis lupus familiaris (dog)	TJP2	NP_001003204.1
Mus musculus (house mouse)	Tjp2	NP_001185914.1
Gallus gallus (chicken)	TJP2	NP_990249.1
Homo sapiens (human)	TJP2	NP_001163887.1
Rattus norvegicus (Norway rat)	Tjp2	NP_446225.1

Gene Ontology
Bibliography

Related articles in PubMed

ZO-1 and -2 Are Required for TRPV1-Modulated Paracellular Permeability.
Li J, Cong X, Zhang Y, Xiang RL, Mei M, Yang NY, Su YC, Choi S, Park K, Zhang LW, Wu LL, Yu GY
Journal of dental research94(12)1748-56(2015 Dec)

Progressive Hearing Loss in Mice Carrying a Mutation in Usp53.
Kazmierczak M, Harris SL, Kazmierczak P, Shah P, Starovoytov V, Ohlemiller KK, Schwander M
The Journal of neuroscience : the official journal of the Society for Neuroscience35(47)15582-98(2015 Nov)

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Guo Z, Neilson LJ, Zhong H, Murray PS, Zanivan S, Zaidel-Bar R
Science signaling7(354)rs7(2014 Dec)

JNK1/2-dependent phosphorylation of angulin-1/LSR is required for the exclusive localization of angulin-1/LSR and tricellulin at tricellular contacts in EpH4 epithelial sheet.
Nakatsu D, Kano F, Taguchi Y, Sugawara T, Nishizono T, Nishikawa K, Oda Y, Furuse M, Murata M
Genes to cells : devoted to molecular & cellular mechanisms19(7)565-81(2014 Jul)

Different effects of ZO-1, ZO-2 and ZO-3 silencing on kidney collecting duct principal cell proliferation and adhesion.
Qiao X, Roth I, F?raille E, Hasler U
Cell cycle (Georgetown, Tex.)13(19)3059-75(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

crucial roles in basal salivary epithelial barrier function and TRPV1-modulated paracellular transport
Title: ZO-1 and -2 Are Required for TRPV1-Modulated Paracellular Permeability.

ZO-2 suppression decreased cyclin D1 abundance and the depletion of which restored cell cycle progression.
Title: Different effects of ZO-1, ZO-2 and ZO-3 silencing on kidney collecting duct principal cell proliferation and adhesion.

Levels of tight junction protein ZO2 are decreased in blood brain barrier cells post-translationally following neurotoxicant administration.
Title: Assessments of tight junction proteins occludin, claudin 5 and scaffold proteins ZO1 and ZO2 in endothelial cells of the rat blood-brain barrier: cellular responses to neurotoxicants malathion and lead acetate.

Fg binding to endothelial cells (ECs) alters expression of actin-associated endothelial tight junction proteins.
Title: Fibrinogen induces alterations of endothelial cell tight junction proteins.

The highly increased expression and changed distribution of ZO-2 suggests that ZO-1 is partly substituted by ZO-2, an alteration possibly causing impaired barrier function.
Title: Bile duct ligation in the rat causes upregulation of ZO-2 and decreased colocalization of claudins with ZO-1 and occludin.

The following Tjp2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tjp2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa10600	NM_053773.1 Latest version!	Rattus norvegicus tight junction protein 2 (Tjp2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
ORa23971	XM_006231175.3 Latest version!	Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
ORa23972	XM_006231176.3 Latest version!	Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
ORa23973	XM_006231177.3 Latest version!	Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
ORa23974	XM_006231178.3 Latest version!	Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
ORa23977	XM_008760302.2 Latest version!	Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
ORa23977	XM_006231181.3 Latest version!	Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
ORa23978	XM_006231182.3 Latest version!	Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tjp2 ( NM_053773.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446225.1 Rattus norvegicus tight junction protein 2 (Tjp2), mRNA.

Price & Availability↑

CloneID	ORa10600
Clone ID Related Accession (Same CDS sequence)	NM_053773.1
Accession Version	NM_053773.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Rattus norvegicus(Norway rat)
Product	tight junction protein ZO-2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC103479.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC103479.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAGGAGG TGATATGGGA ACAGTACACT GTGACCCTGC AGAAGGATTC CAAAAGAGGA 
TTTGGAATCG CAGTGTCAGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACGTCA 
ATCGTCATTT CAGATGTACT CCCAGGTGGA CCCGCTGATG GCTTGCTTCA AGAAAATGAC 
AGGGTGGTCA TGGTCAATGG CACCCCCATG GAGGATGTGC TCCATTCATT TGCCGTTCAG 
CAGCTTAGGA AAAGTGGGAA GATCGCAGCC ATTGTGGTCA AGAGGCCTCG GAAGGTTCAG 
GTGGCCCCGC TGCAGGGCAG CCCCCCTCTC AGTCATGATG ACCGAGGTTT TGAGATGATG 
GATGAATTTG ATGGCAGAAG TTTCCGCAGC GGCTACAGTG AGAGGAGCCG GCACAGCAGC 
CACGATATGC TCAGCCACAG CTGGGAGGAC AGCAGGGAGA GGGGGCGTCC CCATCAGCGC 
GCACAGAGCC GGGAGCGGGA GCGCAGCCGT GGCCGGAGCC TGGAACGTGG CCTGGACCAT 
GAAGACTACG GGCGCAGCCA TGAGCGCAGC CGCGGCCGGA GCCTGGAGCG CGGTCTAGAC 
CGCGACTTTG TGTCCCGGGA CTACAGCCGT GGACGGAGCA TCGACAGGGA CTACGACCGA 
GACTATGAGC GCTCCTATCA CCAAGCCTAT GAGCCTGACT ACGAAGGCCG CTACAGCCCA 
TCTTATGACC GCAGAGCCCA TCCAGAGACC CGCTATGAAC GGAGCCGCAG CCGAGAACAC 
CTACGGTCCC GAAGCCCCAC CCCCGAGTCT AGGTCCCGGC AGGAACACAA GGGCCAGCAT 
GATCCCGACA GGCCCATCGG GGTCCTTCTG ACCAAAAGCA AAGCAAACGA AGAGTACGGG 
CTCCGGCTGG GAAGTCAGAT CTTCATTAAG GAAATGACCA GAACAGGACT GGCAACAAAA 
GATGGCAACC TTCATGAGGG GGACATAATT CTCAAGATCA ATGGAACTGT TACTGAGAAC 
ATGTCTTTAA CCGATGCTCG GAAGTTAATA GAAAAGTCTC GAGGAAAACT GCAGCTGGTG 
GTGTTGAGAG ACAGCAAGCA GACCCTCATC AACATCCCAT CTCTGAATGA CAGCGACTCG 
GAAGTGGAGG ATATCTCGGA AATCGAGTCC AACCGATCTT TTTCTCCAGA GGAGAGGCGC 
CAGCAGTATT CTGATCAGGA GTATCATTCC TCCACTGAGA AGCTGAAGGA GAGGCCAAGC 
TCAAGAGAGG AGACCTCAGG CAGAATGTCC AGGATGGGTG CCACACCCAC GCCGTTCAAG 
TCCACGGGGG ACATCACAGC TGCAGGTGTC ACAGAGGCCA ACAAGGAGCC CAGGTCCCAG 
GAGGAATCCC CAGTTCCTCA ACCCAGAACA GCATCAAGAG TCTTTCTTCG TCCTAGTCCC 
GAAGATGAAG CAATATATGG CCCTAACACC AAAATGGTGA AGTTCAAGAA GGGAGACAGC 
GTGGGCCTCC GGTTGGCTGG TGGAAATGAT GTTGGCATAT TTGTGGCTGG CATTCAGGAG 
GGCACCTCTG CAGAGCAGGA GGGCCTACAA GAAGGGGACC AGATTCTGAA GGTGAACACA 
CAAGATTTCA GAGGGCTGGT CCGGGAAGAT GCCGTCCTCT ACCTGTTAGA AATCCCTAAA 
GGTGAAACCG TGACCATTTT GGCTCAGAGC CGAGCAGACG TGTATAGAGA CATCCTGGCC 
TGTGGCAGGG GAGACTCGTT CTTCATAAGG AGCCACTTTG AATGTGAGAA GGAGACTCCG 
CAGAGCTTGG CCTTCACCAG GGGAGAGGTC TTCCGAGTGG TAGACACGCT GTACGATGGC 
AAACTGGGCC ACTGGCTGGC TGTGAGGATT GGAAATGAGC TGGAGAAGGG CTTAATCCCT 
AACAAAAGCA GAGCTGAGCA GATGGCCAGT GTCCAGAATG CCCAGCGAGA GAATGCTGGG 
GACAGAGCAG ACTTCTGGCG GATGCGTGGC CAGAGATCCG GGGTCAAGAA GAACATTCGC 
AAGAGCCGGG AAGACCTGGC AGCTGCTGTG TCGGTTAGCA CCAAGTTCCC CGCCTACGAG 
AAGGTTCTGC TTCGGGAAGC TGGCTTCAAG AGACCCGTGG TTCTGTTTGG CCCCATAGCA 
GATATAGCAA TGGAAAGGCT GACTACTGAG CTACCCGACC TGTTTCAAAC TGCAAAAACA 
GAACCCAAAG ATGCGGGATC TGAGAAATCC AGTGGAGTGG TTCGGTTGAA TACTGTGAGG 
CAAATTATTG AGCAGGACAA GCATGCCCTG CTCGACGTTA CCCCCAAAGC TGTGGACCTG 
CTCAATTATA CTCAGTGGTT CCCAATCGTG ATTTTCTTCA ACCCGGATTC CAGACAAGGC 
ATTAAAACCA TAAGGCAGAG GTTGAACCCA ACATCCAATA AAAGTTCTCG CAAGTTATTC 
GATCAAGCCA ACAAGCTCAA AAAAACCTGT TCTCATCTTT TTACAGCTAC CATCAACGTG 
AATTCAGCCA ATGATGGCTG GTTTGGCAGC CTGAAGGACA GCATTCAGCA GCAGCAAAAC 
GAAGCAGTGT GGGTCTCTGA AGGAAAGATG GAGGGGATGG ATGATGACGC TGAAGACCGC 
ATGTCCTACT TAACCGCCAT GGGTGCGGAC TATCTGAGTT GTGACAGCCG TCTCATCAGT 
GACTTTGAAG ATACGGACGG CGAGGGAGGC GCCTACACTG ACAATGAGCT GGATGAGCCA 
GCTGAGGAGC CGCTGGTGTC TTCCATCACC CGCTCCTCAG AGCCGGTGCA GCATGAGGAG 
AGCATAAGGA AGCCCAGCCC AGAGCCACGC GCTCAGATGA GGAGGGCAGC TAGCAGAGAC 
CAGCTTAGGG ATGGTAGCCC GCCCCCAGCA TTCAAGCCAG AGCCGCCCAA GGTCAGAAAC 
CAAAACAGAG AGGACTCTTT CAACTACTCC AAGTCAAACC TCTCTGCCAT GGCTGGCAGT 
GAAATCCCGG GGGGATCCAC CAAAGGGTGT CCTCCCCCTA TTGCGGTGAA ACCTGCCTTT 
GGGCGATCCA TCCTGAAGCC TTCTACTCCA GTCCCCATGC CTGAGAGTGA GGAGGTTGGG 
GAGAGCACCG AGGAGCAGGA AGAGGCTCCC AAATCAGTCC TGGGCAGAGT GAAAATCTTC 
GAGAAGATGG ACCACAAGGC GAAATTACAG AGGATGCAGG AGCTCCAAGA AGCACAGAAT 
GCGAGGATTG AAATAGCTCA GAAGCATCCT GACATCTATG CGGTTCCAAT CAAAGCCCCC 
AAGCCAGATG CTGGCCTGCC CCAGCACATG AGACCCCCAG AGCCACAGAA AGCTCCTTCT 
AGGCTTTACC AGGACACCAG AGGAAGCTAC GGCAGTGATC CCGAGGAAGA GGAGGAGTAC 
CGCCAACAGT TGGCAGCACA CTCGAAGCGT GGTTACTACA GCCAGCCCTC CCGGTACCGA 
GACACCGAAT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446225.1
CDS	311..3805
Translation	MEEVIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKIAAIVVKRPRKVQVAPLQGSPPLSHDDRGFEMMDEFDGRSFRSGYSERSRHSSHDMLSHSWEDSRERGRPHQRAQSRERERSRGRSLERGLDHEDYGRSHERSRGRSLERGLDRDFVSRDYSRGRSIDRDYDRDYERSYHQAYEPDYEGRYSPSYDRRAHPETRYERSRSREHLRSRSPTPESRSRQEHKGQHDPDRPIGVLLTKSKANEEYGLRLGSQIFIKEMTRTGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSKQTLINIPSLNDSDSEVEDISEIESNRSFSPEERRQQYSDQEYHSSTEKLKERPSSREETSGRMSRMGATPTPFKSTGDITAAGVTEANKEPRSQEESPVPQPRTASRVFLRPSPEDEAIYGPNTKMVKFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGLVREDAVLYLLEIPKGETVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEVFRVVDTLYDGKLGHWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRENAGDRADFWRMRGQRSGVKKNIRKSREDLAAAVSVSTKFPAYEKVLLREAGFKRPVVLFGPIADIAMERLTTELPDLFQTAKTEPKDAGSEKSSGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGIKTIRQRLNPTSNKSSRKLFDQANKLKKTCSHLFTATINVNSANDGWFGSLKDSIQQQQNEAVWVSEGKMEGMDDDAEDRMSYLTAMGADYLSCDSRLISDFEDTDGEGGAYTDNELDEPAEEPLVSSITRSSEPVQHEESIRKPSPEPRAQMRRAASRDQLRDGSPPPAFKPEPPKVRNQNREDSFNYSKSNLSAMAGSEIPGGSTKGCPPPIAVKPAFGRSILKPSTPVPMPESEEVGESTEEQEEAPKSVLGRVKIFEKMDHKAKLQRMQELQEAQNARIEIAQKHPDIYAVPIKAPKPDAGLPQHMRPPEPQKAPSRLYQDTRGSYGSDPEEEEEYRQQLAAHSKRGYYSQPSRYRDTEL

Target ORF information:

RefSeq Version	NM_053773.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tight junction protein 2 (Tjp2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053773.1

ORF Insert Sequence:

ATGGAGGAGG TGATATGGGA ACAGTACACT GTGACCCTGC AGAAGGATTC CAAAAGAGGA 
TTTGGAATCG CAGTGTCAGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACGTCA 
ATCGTCATTT CAGATGTACT CCCAGGTGGA CCCGCTGATG GCTTGCTTCA AGAAAATGAC 
AGGGTGGTCA TGGTCAATGG CACCCCCATG GAGGATGTGC TCCATTCATT TGCCGTTCAG 
CAGCTTAGGA AAAGTGGGAA GATCGCAGCC ATTGTGGTCA AGAGGCCTCG GAAGGTTCAG 
GTGGCCCCGC TGCAGGGCAG CCCCCCTCTC AGTCATGATG ACCGAGGTTT TGAGATGATG 
GATGAATTTG ATGGCAGAAG TTTCCGCAGC GGCTACAGTG AGAGGAGCCG GCACAGCAGC 
CACGATATGC TCAGCCACAG CTGGGAGGAC AGCAGGGAGA GGGGGCGTCC CCATCAGCGC 
GCACAGAGCC GGGAGCGGGA GCGCAGCCGT GGCCGGAGCC TGGAACGTGG CCTGGACCAT 
GAAGACTACG GGCGCAGCCA TGAGCGCAGC CGCGGCCGGA GCCTGGAGCG CGGTCTAGAC 
CGCGACTTTG TGTCCCGGGA CTACAGCCGT GGACGGAGCA TCGACAGGGA CTACGACCGA 
GACTATGAGC GCTCCTATCA CCAAGCCTAT GAGCCTGACT ACGAAGGCCG CTACAGCCCA 
TCTTATGACC GCAGAGCCCA TCCAGAGACC CGCTATGAAC GGAGCCGCAG CCGAGAACAC 
CTACGGTCCC GAAGCCCCAC CCCCGAGTCT AGGTCCCGGC AGGAACACAA GGGCCAGCAT 
GATCCCGACA GGCCCATCGG GGTCCTTCTG ACCAAAAGCA AAGCAAACGA AGAGTACGGG 
CTCCGGCTGG GAAGTCAGAT CTTCATTAAG GAAATGACCA GAACAGGACT GGCAACAAAA 
GATGGCAACC TTCATGAGGG GGACATAATT CTCAAGATCA ATGGAACTGT TACTGAGAAC 
ATGTCTTTAA CCGATGCTCG GAAGTTAATA GAAAAGTCTC GAGGAAAACT GCAGCTGGTG 
GTGTTGAGAG ACAGCAAGCA GACCCTCATC AACATCCCAT CTCTGAATGA CAGCGACTCG 
GAAGTGGAGG ATATCTCGGA AATCGAGTCC AACCGATCTT TTTCTCCAGA GGAGAGGCGC 
CAGCAGTATT CTGATCAGGA GTATCATTCC TCCACTGAGA AGCTGAAGGA GAGGCCAAGC 
TCAAGAGAGG AGACCTCAGG CAGAATGTCC AGGATGGGTG CCACACCCAC GCCGTTCAAG 
TCCACGGGGG ACATCACAGC TGCAGGTGTC ACAGAGGCCA ACAAGGAGCC CAGGTCCCAG 
GAGGAATCCC CAGTTCCTCA ACCCAGAACA GCATCAAGAG TCTTTCTTCG TCCTAGTCCC 
GAAGATGAAG CAATATATGG CCCTAACACC AAAATGGTGA AGTTCAAGAA GGGAGACAGC 
GTGGGCCTCC GGTTGGCTGG TGGAAATGAT GTTGGCATAT TTGTGGCTGG CATTCAGGAG 
GGCACCTCTG CAGAGCAGGA GGGCCTACAA GAAGGGGACC AGATTCTGAA GGTGAACACA 
CAAGATTTCA GAGGGCTGGT CCGGGAAGAT GCCGTCCTCT ACCTGTTAGA AATCCCTAAA 
GGTGAAACCG TGACCATTTT GGCTCAGAGC CGAGCAGACG TGTATAGAGA CATCCTGGCC 
TGTGGCAGGG GAGACTCGTT CTTCATAAGG AGCCACTTTG AATGTGAGAA GGAGACTCCG 
CAGAGCTTGG CCTTCACCAG GGGAGAGGTC TTCCGAGTGG TAGACACGCT GTACGATGGC 
AAACTGGGCC ACTGGCTGGC TGTGAGGATT GGAAATGAGC TGGAGAAGGG CTTAATCCCT 
AACAAAAGCA GAGCTGAGCA GATGGCCAGT GTCCAGAATG CCCAGCGAGA GAATGCTGGG 
GACAGAGCAG ACTTCTGGCG GATGCGTGGC CAGAGATCCG GGGTCAAGAA GAACATTCGC 
AAGAGCCGGG AAGACCTGGC AGCTGCTGTG TCGGTTAGCA CCAAGTTCCC CGCCTACGAG 
AAGGTTCTGC TTCGGGAAGC TGGCTTCAAG AGACCCGTGG TTCTGTTTGG CCCCATAGCA 
GATATAGCAA TGGAAAGGCT GACTACTGAG CTACCCGACC TGTTTCAAAC TGCAAAAACA 
GAACCCAAAG ATGCGGGATC TGAGAAATCC AGTGGAGTGG TTCGGTTGAA TACTGTGAGG 
CAAATTATTG AGCAGGACAA GCATGCCCTG CTCGACGTTA CCCCCAAAGC TGTGGACCTG 
CTCAATTATA CTCAGTGGTT CCCAATCGTG ATTTTCTTCA ACCCGGATTC CAGACAAGGC 
ATTAAAACCA TAAGGCAGAG GTTGAACCCA ACATCCAATA AAAGTTCTCG CAAGTTATTC 
GATCAAGCCA ACAAGCTCAA AAAAACCTGT TCTCATCTTT TTACAGCTAC CATCAACGTG 
AATTCAGCCA ATGATGGCTG GTTTGGCAGC CTGAAGGACA GCATTCAGCA GCAGCAAAAC 
GAAGCAGTGT GGGTCTCTGA AGGAAAGATG GAGGGGATGG ATGATGACGC TGAAGACCGC 
ATGTCCTACT TAACCGCCAT GGGTGCGGAC TATCTGAGTT GTGACAGCCG TCTCATCAGT 
GACTTTGAAG ATACGGACGG CGAGGGAGGC GCCTACACTG ACAATGAGCT GGATGAGCCA 
GCTGAGGAGC CGCTGGTGTC TTCCATCACC CGCTCCTCAG AGCCGGTGCA GCATGAGGAG 
AGCATAAGGA AGCCCAGCCC AGAGCCACGC GCTCAGATGA GGAGGGCAGC TAGCAGAGAC 
CAGCTTAGGG ATGGTAGCCC GCCCCCAGCA TTCAAGCCAG AGCCGCCCAA GGTCAGAAAC 
CAAAACAGAG AGGACTCTTT CAACTACTCC AAGTCAAACC TCTCTGCCAT GGCTGGCAGT 
GAAATCCCGG GGGGATCCAC CAAAGGGTGT CCTCCCCCTA TTGCGGTGAA ACCTGCCTTT 
GGGCGATCCA TCCTGAAGCC TTCTACTCCA GTCCCCATGC CTGAGAGTGA GGAGGTTGGG 
GAGAGCACCG AGGAGCAGGA AGAGGCTCCC AAATCAGTCC TGGGCAGAGT GAAAATCTTC 
GAGAAGATGG ACCACAAGGC GAAATTACAG AGGATGCAGG AGCTCCAAGA AGCACAGAAT 
GCGAGGATTG AAATAGCTCA GAAGCATCCT GACATCTATG CGGTTCCAAT CAAAGCCCCC 
AAGCCAGATG CTGGCCTGCC CCAGCACATG AGACCCCCAG AGCCACAGAA AGCTCCTTCT 
AGGCTTTACC AGGACACCAG AGGAAGCTAC GGCAGTGATC CCGAGGAAGA GGAGGAGTAC 
CGCCAACAGT TGGCAGCACA CTCGAAGCGT GGTTACTACA GCCAGCCCTC CCGGTACCGA 
GACACCGAAT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp2 ( XM_006231175.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231237.1 Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa23971
Clone ID Related Accession (Same CDS sequence)	XM_006231175.3
Accession Version	XM_006231175.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tight junction protein ZO-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231175.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACTGT ATACCGAGAA AGACAAGATG AGATACAAGA AATACATGGC TATTCTGGAA 
GCGGCAGTGG GCATTACCCC ACTTAACAAA AGAGAGCTTC TACCCGAGAG CCGGAGACAC 
GTAAACCTTT GGCAGCACCC GGGGGCCCGG AGTCCTGCCT TGCTCTCTTC TCCCTGTCAG 
TGGGACTGGC ACCCAGGATA CAGCTCCAGG CCCAAGCACT CTGCAGCGCA CAGACCGGTG 
TCCACCTACA GCCTGCTCTC CCAGGGCCCA GGCATGGAGG AGGTGATATG GGAACAGTAC 
ACTGTGACCC TGCAGAAGGA TTCCAAAAGA GGATTTGGAA TCGCAGTGTC AGGAGGCAGA 
GACAACCCCC ACTTTGAAAA TGGGGAGACG TCAATCGTCA TTTCAGATGT ACTCCCAGGT 
GGACCCGCTG ATGGCTTGCT TCAAGAAAAT GACAGGGTGG TCATGGTCAA TGGCACCCCC 
ATGGAGGATG TGCTCCATTC ATTTGCCGTT CAGCAGCTTA GGAAAAGTGG GAAGATCGCA 
GCCATTGTGG TCAAGAGGCC TCGGAAGGTT CAGGTGGCCC CGCTGCAGGG CAGCCCCCCT 
CTCAGTCATG ATGACCGAGG TTTTGAGATG ATGGATGAAT TTGATGGCAG AAGTTTCCGC 
AGCGGCTACA GTGAGAGGAG CCGGCACAGC AGCCACGATA TGCTCAGCCA CAGCTGGGAG 
GACAGCAGGG AGAGGGGGCG TCCCCATCAG CGCGCACAGA GCCGGGAGCG GGAGCGCAGC 
CGTGGCCGGA GCCTGGAACG TGGCCTGGAC CATGAAGACT ACGGGCGCAG CCATGAGCGC 
AGCCGCGGCC GGAGCCTGGA GCGCGGTCTA GACCGCGACT TTGTGTCCCG GGACTACAGC 
CGTGGACGGA GCATCGACAG GGACTACGAC CGAGACTATG AGCGCTCCTA TCACCAAGCC 
TATGAGCCTG ACTACGAAGG CCGCTACAGC CCATCTTATG ACCGCAGAGC CCATCCAGAG 
ACCCGCTATG AACGGAGCCG CAGCCGAGAA CACCTACGGT CCCGAAGCCC CACCCCCGAG 
TCTAGGTCCC GGCAGGAACA CAAGGGCCAG CATGATCCCG ACAGGCCCAT CGGGGTCCTT 
CTGACCAAAA GCAAAGCAAA CGAAGAGTAC GGGCTCCGGC TGGGAAGTCA GATCTTCATT 
AAGGAAATGA CCAGAACAGG ACTGGCAACA AAAGATGGCA ACCTTCATGA GGGGGACATA 
ATTCTCAAGA TCAATGGAAC TGTTACTGAG AACATGTCTT TAACCGATGC TCGGAAGTTA 
ATAGAAAAGT CTCGAGGAAA ACTGCAGCTG GTGGTGTTGA GAGACAGCAA GCAGACCCTC 
ATCAACATCC CATCTCTGAA TGACAGCGAC TCGGAAGTGG AGGATATCTC GGAAATCGAG 
TCCAACCGAT CTTTTTCTCC AGAGGAGAGG CGCCAGCAGT ATTCTGATCA GGAGTATCAT 
TCCTCCACTG AGAAGCTGAA GGAGAGGCCA AGCTCAAGAG AGGAGACCTC AGGCAGAATG 
TCCAGGATGG GTGCCACACC CACGCCGTTC AAGTCCACGG GGGACATCAC AGCTGCAGGT 
GTCACAGAGG CCAACAAGGA GCCCAGGTCC CAGGAGGAAT CCCCAGTTCC TCAACCCAGA 
ACAGCATCAA GAGTCTTTCT TCGTCCTAGT CCCGAAGATG AAGCAATATA TGGCCCTAAC 
ACCAAAATGG TGAAGTTCAA GAAGGGAGAC AGCGTGGGCC TCCGGTTGGC TGGTGGAAAT 
GATGTTGGCA TATTTGTGGC TGGCATTCAG GAGGGCACCT CTGCAGAGCA GGAGGGCCTA 
CAAGAAGGGG ACCAGATTCT GAAGGTGAAC ACACAAGATT TCAGAGGGCT GGTCCGGGAA 
GATGCCGTCC TCTACCTGTT AGAAATCCCT AAAGGTGAAA CCGTGACCAT TTTGGCTCAG 
AGCCGAGCAG ACGTGTATAG AGACATCCTG GCCTGTGGCA GGGGAGACTC GTTCTTCATA 
AGGAGCCACT TTGAATGTGA GAAGGAGACT CCGCAGAGCT TGGCCTTCAC CAGGGGAGAG 
GTCTTCCGAG TGGTAGACAC GCTGTACGAT GGCAAACTGG GCCACTGGCT GGCTGTGAGG 
ATTGGAAATG AGCTGGAGAA GGGCTTAATC CCTAACAAAA GCAGAGCTGA GCAGATGGCC 
AGTGTCCAGA ATGCCCAGCG AGAGAATGCT GGGGACAGAG CAGACTTCTG GCGGATGCGT 
GGCCAGAGAT CCGGGGTCAA GAAGAACATT CGCAAGAGCC GGGAAGACCT GGCAGCTGCT 
GTGTCGGTTA GCACCAAGTT CCCCGCCTAC GAGAAGGTTC TGCTTCGGGA AGCTGGCTTC 
AAGAGACCCG TGGTTCTGTT TGGCCCCATA GCAGATATAG CAATGGAAAG GCTGACTACT 
GAGCTACCCG ACCTGTTTCA AACTGCAAAA ACAGAACCCA AAGATGCGGG ATCTGAGAAA 
TCCAGTGGAG TGGTTCGGTT GAATACTGTG AGGCAAATTA TTGAGCAGGA CAAGCATGCC 
CTGCTCGACG TTACCCCCAA AGCTGTGGAC CTGCTCAATT ATACTCAGTG GTTCCCAATC 
GTGATTTTCT TCAACCCGGA TTCCAGACAA GGCATTAAAA CCATAAGGCA GAGGTTGAAC 
CCAACATCCA ATAAAAGTTC TCGCAAGTTA TTCGATCAAG CCAACAAGCT CAAAAAAACC 
TGTTCTCATC TTTTTACAGC TACCATCAAC GTGAATTCAG CCAATGATGG CTGGTTTGGC 
AGCCTGAAGG ACAGCATTCA GCAGCAGCAA AACGAAGCAG TGTGGGTCTC TGAAGGAAAG 
ATGGAGGGGA TGGATGATGA CGCTGAAGAC CGCATGTCCT ACTTAACCGC CATGGGTGCG 
GACTATCTGA GTTGTGACAG CCGTCTCATC AGTGACTTTG AAGATACGGA CGGCGAGGGA 
GGCGCCTACA CTGACAATGA GCTGGATGAG CCAGCTGAGG AGCCGCTGGT GTCTTCCATC 
ACCCGCTCCT CAGAGCCGGT GCAGCATGAG GAGAGCATAA GGAAGCCCAG CCCAGAGCCA 
CGCGCTCAGA TGAGGAGGGC AGCTAGCAGA GACCAGCTTA GGGATGGTAG CCCGCCCCCA 
GCATTCAAGC CAGAGCCGCC CAAGGTCAGA AACCAAAACA GAGAGGACTC TTTCAACTAC 
TCCAAGTCAA ACCTCTCTGC CATGGCTGGC AGTGAAATCC CGGGGGGATC CACCAAAGGG 
TGTCCTCCCC CTATTGCGGT GAAACCTGCC TTTGGGCGAT CCATCCTGAA GCCTTCTACT 
CCAGTCCCCA TGCCTGAGAG TGAGGAGGTT GGGGAGAGCA CCGAGGAGCA GGAAGAGGCT 
CCCAAATCAG TCCTGGGCAG AGTGAAAATC TTCGAGAAGA TGGACCACAA GGCGAAATTA 
CAGAGGATGC AGGAGCTCCA AGAAGCACAG AATGCGAGGA TTGAAATAGC TCAGAAGCAT 
CCTGACATCT ATGCGGTTCC AATCAAAGCC CCCAAGCCAG ATGCTGGCCT GCCCCAGCAC 
ATGAGTTCTA GACCCCCAGA GCCACAGAAA GCTCCTTCTA GGCTTTACCA GGACACCAGA 
GGAAGCTACG GCAGTGATCC CGAGGAAGAG GAGGAGTACC GCCAACAGTT GGCAGCACAC 
TCGAAGCGTG GTTACTACAG CCAGCCCTCC CGGTACCGAG ACACCGAATT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231237.1
CDS	555..4328
Translation	MGLYTEKDKMRYKKYMAILEAAVGITPLNKRELLPESRRHVNLWQHPGARSPALLSSPCQWDWHPGYSSRPKHSAAHRPVSTYSLLSQGPGMEEVIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKIAAIVVKRPRKVQVAPLQGSPPLSHDDRGFEMMDEFDGRSFRSGYSERSRHSSHDMLSHSWEDSRERGRPHQRAQSRERERSRGRSLERGLDHEDYGRSHERSRGRSLERGLDRDFVSRDYSRGRSIDRDYDRDYERSYHQAYEPDYEGRYSPSYDRRAHPETRYERSRSREHLRSRSPTPESRSRQEHKGQHDPDRPIGVLLTKSKANEEYGLRLGSQIFIKEMTRTGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSKQTLINIPSLNDSDSEVEDISEIESNRSFSPEERRQQYSDQEYHSSTEKLKERPSSREETSGRMSRMGATPTPFKSTGDITAAGVTEANKEPRSQEESPVPQPRTASRVFLRPSPEDEAIYGPNTKMVKFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGLVREDAVLYLLEIPKGETVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEVFRVVDTLYDGKLGHWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRENAGDRADFWRMRGQRSGVKKNIRKSREDLAAAVSVSTKFPAYEKVLLREAGFKRPVVLFGPIADIAMERLTTELPDLFQTAKTEPKDAGSEKSSGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGIKTIRQRLNPTSNKSSRKLFDQANKLKKTCSHLFTATINVNSANDGWFGSLKDSIQQQQNEAVWVSEGKMEGMDDDAEDRMSYLTAMGADYLSCDSRLISDFEDTDGEGGAYTDNELDEPAEEPLVSSITRSSEPVQHEESIRKPSPEPRAQMRRAASRDQLRDGSPPPAFKPEPPKVRNQNREDSFNYSKSNLSAMAGSEIPGGSTKGCPPPIAVKPAFGRSILKPSTPVPMPESEEVGESTEEQEEAPKSVLGRVKIFEKMDHKAKLQRMQELQEAQNARIEIAQKHPDIYAVPIKAPKPDAGLPQHMSSRPPEPQKAPSRLYQDTRGSYGSDPEEEEEYRQQLAAHSKRGYYSQPSRYRDTEL

Target ORF information:

RefSeq Version	XM_006231175.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231175.3

ORF Insert Sequence:

ATGGGACTGT ATACCGAGAA AGACAAGATG AGATACAAGA AATACATGGC TATTCTGGAA 
GCGGCAGTGG GCATTACCCC ACTTAACAAA AGAGAGCTTC TACCCGAGAG CCGGAGACAC 
GTAAACCTTT GGCAGCACCC GGGGGCCCGG AGTCCTGCCT TGCTCTCTTC TCCCTGTCAG 
TGGGACTGGC ACCCAGGATA CAGCTCCAGG CCCAAGCACT CTGCAGCGCA CAGACCGGTG 
TCCACCTACA GCCTGCTCTC CCAGGGCCCA GGCATGGAGG AGGTGATATG GGAACAGTAC 
ACTGTGACCC TGCAGAAGGA TTCCAAAAGA GGATTTGGAA TCGCAGTGTC AGGAGGCAGA 
GACAACCCCC ACTTTGAAAA TGGGGAGACG TCAATCGTCA TTTCAGATGT ACTCCCAGGT 
GGACCCGCTG ATGGCTTGCT TCAAGAAAAT GACAGGGTGG TCATGGTCAA TGGCACCCCC 
ATGGAGGATG TGCTCCATTC ATTTGCCGTT CAGCAGCTTA GGAAAAGTGG GAAGATCGCA 
GCCATTGTGG TCAAGAGGCC TCGGAAGGTT CAGGTGGCCC CGCTGCAGGG CAGCCCCCCT 
CTCAGTCATG ATGACCGAGG TTTTGAGATG ATGGATGAAT TTGATGGCAG AAGTTTCCGC 
AGCGGCTACA GTGAGAGGAG CCGGCACAGC AGCCACGATA TGCTCAGCCA CAGCTGGGAG 
GACAGCAGGG AGAGGGGGCG TCCCCATCAG CGCGCACAGA GCCGGGAGCG GGAGCGCAGC 
CGTGGCCGGA GCCTGGAACG TGGCCTGGAC CATGAAGACT ACGGGCGCAG CCATGAGCGC 
AGCCGCGGCC GGAGCCTGGA GCGCGGTCTA GACCGCGACT TTGTGTCCCG GGACTACAGC 
CGTGGACGGA GCATCGACAG GGACTACGAC CGAGACTATG AGCGCTCCTA TCACCAAGCC 
TATGAGCCTG ACTACGAAGG CCGCTACAGC CCATCTTATG ACCGCAGAGC CCATCCAGAG 
ACCCGCTATG AACGGAGCCG CAGCCGAGAA CACCTACGGT CCCGAAGCCC CACCCCCGAG 
TCTAGGTCCC GGCAGGAACA CAAGGGCCAG CATGATCCCG ACAGGCCCAT CGGGGTCCTT 
CTGACCAAAA GCAAAGCAAA CGAAGAGTAC GGGCTCCGGC TGGGAAGTCA GATCTTCATT 
AAGGAAATGA CCAGAACAGG ACTGGCAACA AAAGATGGCA ACCTTCATGA GGGGGACATA 
ATTCTCAAGA TCAATGGAAC TGTTACTGAG AACATGTCTT TAACCGATGC TCGGAAGTTA 
ATAGAAAAGT CTCGAGGAAA ACTGCAGCTG GTGGTGTTGA GAGACAGCAA GCAGACCCTC 
ATCAACATCC CATCTCTGAA TGACAGCGAC TCGGAAGTGG AGGATATCTC GGAAATCGAG 
TCCAACCGAT CTTTTTCTCC AGAGGAGAGG CGCCAGCAGT ATTCTGATCA GGAGTATCAT 
TCCTCCACTG AGAAGCTGAA GGAGAGGCCA AGCTCAAGAG AGGAGACCTC AGGCAGAATG 
TCCAGGATGG GTGCCACACC CACGCCGTTC AAGTCCACGG GGGACATCAC AGCTGCAGGT 
GTCACAGAGG CCAACAAGGA GCCCAGGTCC CAGGAGGAAT CCCCAGTTCC TCAACCCAGA 
ACAGCATCAA GAGTCTTTCT TCGTCCTAGT CCCGAAGATG AAGCAATATA TGGCCCTAAC 
ACCAAAATGG TGAAGTTCAA GAAGGGAGAC AGCGTGGGCC TCCGGTTGGC TGGTGGAAAT 
GATGTTGGCA TATTTGTGGC TGGCATTCAG GAGGGCACCT CTGCAGAGCA GGAGGGCCTA 
CAAGAAGGGG ACCAGATTCT GAAGGTGAAC ACACAAGATT TCAGAGGGCT GGTCCGGGAA 
GATGCCGTCC TCTACCTGTT AGAAATCCCT AAAGGTGAAA CCGTGACCAT TTTGGCTCAG 
AGCCGAGCAG ACGTGTATAG AGACATCCTG GCCTGTGGCA GGGGAGACTC GTTCTTCATA 
AGGAGCCACT TTGAATGTGA GAAGGAGACT CCGCAGAGCT TGGCCTTCAC CAGGGGAGAG 
GTCTTCCGAG TGGTAGACAC GCTGTACGAT GGCAAACTGG GCCACTGGCT GGCTGTGAGG 
ATTGGAAATG AGCTGGAGAA GGGCTTAATC CCTAACAAAA GCAGAGCTGA GCAGATGGCC 
AGTGTCCAGA ATGCCCAGCG AGAGAATGCT GGGGACAGAG CAGACTTCTG GCGGATGCGT 
GGCCAGAGAT CCGGGGTCAA GAAGAACATT CGCAAGAGCC GGGAAGACCT GGCAGCTGCT 
GTGTCGGTTA GCACCAAGTT CCCCGCCTAC GAGAAGGTTC TGCTTCGGGA AGCTGGCTTC 
AAGAGACCCG TGGTTCTGTT TGGCCCCATA GCAGATATAG CAATGGAAAG GCTGACTACT 
GAGCTACCCG ACCTGTTTCA AACTGCAAAA ACAGAACCCA AAGATGCGGG ATCTGAGAAA 
TCCAGTGGAG TGGTTCGGTT GAATACTGTG AGGCAAATTA TTGAGCAGGA CAAGCATGCC 
CTGCTCGACG TTACCCCCAA AGCTGTGGAC CTGCTCAATT ATACTCAGTG GTTCCCAATC 
GTGATTTTCT TCAACCCGGA TTCCAGACAA GGCATTAAAA CCATAAGGCA GAGGTTGAAC 
CCAACATCCA ATAAAAGTTC TCGCAAGTTA TTCGATCAAG CCAACAAGCT CAAAAAAACC 
TGTTCTCATC TTTTTACAGC TACCATCAAC GTGAATTCAG CCAATGATGG CTGGTTTGGC 
AGCCTGAAGG ACAGCATTCA GCAGCAGCAA AACGAAGCAG TGTGGGTCTC TGAAGGAAAG 
ATGGAGGGGA TGGATGATGA CGCTGAAGAC CGCATGTCCT ACTTAACCGC CATGGGTGCG 
GACTATCTGA GTTGTGACAG CCGTCTCATC AGTGACTTTG AAGATACGGA CGGCGAGGGA 
GGCGCCTACA CTGACAATGA GCTGGATGAG CCAGCTGAGG AGCCGCTGGT GTCTTCCATC 
ACCCGCTCCT CAGAGCCGGT GCAGCATGAG GAGAGCATAA GGAAGCCCAG CCCAGAGCCA 
CGCGCTCAGA TGAGGAGGGC AGCTAGCAGA GACCAGCTTA GGGATGGTAG CCCGCCCCCA 
GCATTCAAGC CAGAGCCGCC CAAGGTCAGA AACCAAAACA GAGAGGACTC TTTCAACTAC 
TCCAAGTCAA ACCTCTCTGC CATGGCTGGC AGTGAAATCC CGGGGGGATC CACCAAAGGG 
TGTCCTCCCC CTATTGCGGT GAAACCTGCC TTTGGGCGAT CCATCCTGAA GCCTTCTACT 
CCAGTCCCCA TGCCTGAGAG TGAGGAGGTT GGGGAGAGCA CCGAGGAGCA GGAAGAGGCT 
CCCAAATCAG TCCTGGGCAG AGTGAAAATC TTCGAGAAGA TGGACCACAA GGCGAAATTA 
CAGAGGATGC AGGAGCTCCA AGAAGCACAG AATGCGAGGA TTGAAATAGC TCAGAAGCAT 
CCTGACATCT ATGCGGTTCC AATCAAAGCC CCCAAGCCAG ATGCTGGCCT GCCCCAGCAC 
ATGAGTTCTA GACCCCCAGA GCCACAGAAA GCTCCTTCTA GGCTTTACCA GGACACCAGA 
GGAAGCTACG GCAGTGATCC CGAGGAAGAG GAGGAGTACC GCCAACAGTT GGCAGCACAC 
TCGAAGCGTG GTTACTACAG CCAGCCCTCC CGGTACCGAG ACACCGAATT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp2 ( XM_006231176.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231238.1 Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X2, mRNA.

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CloneID	ORa23972
Clone ID Related Accession (Same CDS sequence)	XM_006231176.3
Accession Version	XM_006231176.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tight junction protein ZO-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231176.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACTGT ATACCGAGAA AGACAAGATG AGATACAAGA AATACATGGC TATTCTGGAA 
GCGGCAGTGG GCATTACCCC ACTTAACAAA AGAGAGCTTC TACCCGAGAG CCGGAGACAC 
GTAAACCTTT GGCAGCACCC GGGGGCCCGG AGTCCTGCCT TGCTCTCTTC TCCCTGTCAG 
TGGGACTGGC ACCCAGGATA CAGCTCCAGG CCCAAGCACT CTGCAGCGCA CAGACCGGTG 
TCCACCTACA GCCTGCTCTC CCAGGGCCCA GGCATGGAGG AGGTGATATG GGAACAGTAC 
ACTGTGACCC TGCAGAAGGA TTCCAAAAGA GGATTTGGAA TCGCAGTGTC AGGAGGCAGA 
GACAACCCCC ACTTTGAAAA TGGGGAGACG TCAATCGTCA TTTCAGATGT ACTCCCAGGT 
GGACCCGCTG ATGGCTTGCT TCAAGAAAAT GACAGGGTGG TCATGGTCAA TGGCACCCCC 
ATGGAGGATG TGCTCCATTC ATTTGCCGTT CAGCAGCTTA GGAAAAGTGG GAAGATCGCA 
GCCATTGTGG TCAAGAGGCC TCGGAAGGTT CAGGTGGCCC CGCTGCAGGG CAGCCCCCCT 
CTCAGTCATG ATGACCGAGG TTTTGAGATG ATGGATGAAT TTGATGGCAG AAGTTTCCGC 
AGCGGCTACA GTGAGAGGAG CCGGCACAGC AGCCACGATA TGCTCAGCCA CAGCTGGGAG 
GACAGCAGGG AGAGGGGGCG TCCCCATCAG CGCGCACAGA GCCGGGAGCG GGAGCGCAGC 
CGTGGCCGGA GCCTGGAACG TGGCCTGGAC CATGAAGACT ACGGGCGCAG CCATGAGCGC 
AGCCGCGGCC GGAGCCTGGA GCGCGGTCTA GACCGCGACT TTGTGTCCCG GGACTACAGC 
CGTGGACGGA GCATCGACAG GGACTACGAC CGAGACTATG AGCGCTCCTA TCACCAAGCC 
TATGAGCCTG ACTACGAAGG CCGCTACAGC CCATCTTATG ACCGCAGAGC CCATCCAGAG 
ACCCGCTATG AACGGAGCCG CAGCCGAGAA CACCTACGGT CCCGAAGCCC CACCCCCGAG 
TCTAGGTCCC GGCAGGAACA CAAGGGCCAG CATGATCCCG ACAGGCCCAT CGGGGTCCTT 
CTGACCAAAA GCAAAGCAAA CGAAGAGTAC GGGCTCCGGC TGGGAAGTCA GATCTTCATT 
AAGGAAATGA CCAGAACAGG ACTGGCAACA AAAGATGGCA ACCTTCATGA GGGGGACATA 
ATTCTCAAGA TCAATGGAAC TGTTACTGAG AACATGTCTT TAACCGATGC TCGGAAGTTA 
ATAGAAAAGT CTCGAGGAAA ACTGCAGCTG GTGGTGTTGA GAGACAGCAA GCAGACCCTC 
ATCAACATCC CATCTCTGAA TGACAGCGAC TCGGAAGTGG AGGATATCTC GGAAATCGAG 
TCCAACCGAT CTTTTTCTCC AGAGGAGAGG CGCCAGCAGT ATTCTGATCA GGAGTATCAT 
TCCTCCACTG AGAAGCTGAA GGAGAGGCCA AGCTCAAGAG AGGAGACCTC AGGCAGAATG 
TCCAGGATGG GTGCCACACC CACGCCGTTC AAGTCCACGG GGGACATCAC AGCTGCAGGT 
GTCACAGAGG CCAACAAGGA GCCCAGGTCC CAGGAGGAAT CCCCAGTTCC TCAACCCAGA 
ACAGCATCAA GAGTCTTTCT TCGTCCTAGT CCCGAAGATG AAGCAATATA TGGCCCTAAC 
ACCAAAATGG TGAAGTTCAA GAAGGGAGAC AGCGTGGGCC TCCGGTTGGC TGGTGGAAAT 
GATGTTGGCA TATTTGTGGC TGGCATTCAG GAGGGCACCT CTGCAGAGCA GGAGGGCCTA 
CAAGAAGGGG ACCAGATTCT GAAGGTGAAC ACACAAGATT TCAGAGGGCT GGTCCGGGAA 
GATGCCGTCC TCTACCTGTT AGAAATCCCT AAAGGTGAAA CCGTGACCAT TTTGGCTCAG 
AGCCGAGCAG ACGTGTATAG AGACATCCTG GCCTGTGGCA GGGGAGACTC GTTCTTCATA 
AGGAGCCACT TTGAATGTGA GAAGGAGACT CCGCAGAGCT TGGCCTTCAC CAGGGGAGAG 
GTCTTCCGAG TGGTAGACAC GCTGTACGAT GGCAAACTGG GCCACTGGCT GGCTGTGAGG 
ATTGGAAATG AGCTGGAGAA GGGCTTAATC CCTAACAAAA GCAGAGCTGA GCAGATGGCC 
AGTGTCCAGA ATGCCCAGCG AGAGAATGCT GGGGACAGAG CAGACTTCTG GCGGATGCGT 
GGCCAGAGAT CCGGGGTCAA GAAGAACATT CGCAAGAGCC GGGAAGACCT GGCAGCTGCT 
GTGTCGGTTA GCACCAAGTT CCCCGCCTAC GAGAAGGTTC TGCTTCGGGA AGCTGGCTTC 
AAGAGACCCG TGGTTCTGTT TGGCCCCATA GCAGATATAG CAATGGAAAG GCTGACTACT 
GAGCTACCCG ACCTGTTTCA AACTGCAAAA ACAGAACCCA AAGATGCGGG ATCTGAGAAA 
TCCAGTGGAG TGGTTCGGTT GAATACTGTG AGGCAAATTA TTGAGCAGGA CAAGCATGCC 
CTGCTCGACG TTACCCCCAA AGCTGTGGAC CTGCTCAATT ATACTCAGTG GTTCCCAATC 
GTGATTTTCT TCAACCCGGA TTCCAGACAA GGCATTAAAA CCATAAGGCA GAGGTTGAAC 
CCAACATCCA ATAAAAGTTC TCGCAAGTTA TTCGATCAAG CCAACAAGCT CAAAAAAACC 
TGTTCTCATC TTTTTACAGC TACCATCAAC GTGAATTCAG CCAATGATGG CTGGTTTGGC 
AGCCTGAAGG ACAGCATTCA GCAGCAGCAA AACGAAGCAG TGTGGGTCTC TGAAGGAAAG 
ATGGAGGGGA TGGATGATGA CGCTGAAGAC CGCATGTCCT ACTTAACCGC CATGGGTGCG 
GACTATCTGA GTTGTGACAG CCGTCTCATC AGTGACTTTG AAGATACGGA CGGCGAGGGA 
GGCGCCTACA CTGACAATGA GCTGGATGAG CCAGCTGAGG AGCCGCTGGT GTCTTCCATC 
ACCCGCTCCT CAGAGCCGGT GCAGCATGAG GAGGTCAGAA ACCAAAACAG AGAGGACTCT 
TTCAACTACT CCAAGTCAAA CCTCTCTGCC ATGGCTGGCA GTGAAATCCC GGGGGGATCC 
ACCAAAGGGT GTCCTCCCCC TATTGCGGTG AAACCTGCCT TTGGGCGATC CATCCTGAAG 
CCTTCTACTC CAGTCCCCAT GCCTGAGAGT GAGGAGGTTG GGGAGAGCAC CGAGGAGCAG 
GAAGAGGCTC CCAAATCAGT CCTGGGCAGA GTGAAAATCT TCGAGAAGAT GGACCACAAG 
GCGAAATTAC AGAGGATGCA GGAGCTCCAA GAAGCACAGA ATGCGAGGAT TGAAATAGCT 
CAGAAGCATC CTGACATCTA TGCGGTTCCA ATCAAAGCCC CCAAGCCAGA TGCTGGCCTG 
CCCCAGCACA TGAGTTCTAG ACCCCCAGAG CCACAGAAAG CTCCTTCTAG GCTTTACCAG 
GACACCAGAG GAAGCTACGG CAGTGATCCC GAGGAAGAGG AGGAGTACCG CCAACAGTTG 
GCAGCACACT CGAAGCGTGG TTACTACAGC CAGCCCTCCC GGTACCGAGA CACCGAATTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231238.1
CDS	555..4217
Translation	MGLYTEKDKMRYKKYMAILEAAVGITPLNKRELLPESRRHVNLWQHPGARSPALLSSPCQWDWHPGYSSRPKHSAAHRPVSTYSLLSQGPGMEEVIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKIAAIVVKRPRKVQVAPLQGSPPLSHDDRGFEMMDEFDGRSFRSGYSERSRHSSHDMLSHSWEDSRERGRPHQRAQSRERERSRGRSLERGLDHEDYGRSHERSRGRSLERGLDRDFVSRDYSRGRSIDRDYDRDYERSYHQAYEPDYEGRYSPSYDRRAHPETRYERSRSREHLRSRSPTPESRSRQEHKGQHDPDRPIGVLLTKSKANEEYGLRLGSQIFIKEMTRTGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSKQTLINIPSLNDSDSEVEDISEIESNRSFSPEERRQQYSDQEYHSSTEKLKERPSSREETSGRMSRMGATPTPFKSTGDITAAGVTEANKEPRSQEESPVPQPRTASRVFLRPSPEDEAIYGPNTKMVKFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGLVREDAVLYLLEIPKGETVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEVFRVVDTLYDGKLGHWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRENAGDRADFWRMRGQRSGVKKNIRKSREDLAAAVSVSTKFPAYEKVLLREAGFKRPVVLFGPIADIAMERLTTELPDLFQTAKTEPKDAGSEKSSGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGIKTIRQRLNPTSNKSSRKLFDQANKLKKTCSHLFTATINVNSANDGWFGSLKDSIQQQQNEAVWVSEGKMEGMDDDAEDRMSYLTAMGADYLSCDSRLISDFEDTDGEGGAYTDNELDEPAEEPLVSSITRSSEPVQHEEVRNQNREDSFNYSKSNLSAMAGSEIPGGSTKGCPPPIAVKPAFGRSILKPSTPVPMPESEEVGESTEEQEEAPKSVLGRVKIFEKMDHKAKLQRMQELQEAQNARIEIAQKHPDIYAVPIKAPKPDAGLPQHMSSRPPEPQKAPSRLYQDTRGSYGSDPEEEEEYRQQLAAHSKRGYYSQPSRYRDTEL

Target ORF information:

RefSeq Version	XM_006231176.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231176.3

ORF Insert Sequence:

ATGGGACTGT ATACCGAGAA AGACAAGATG AGATACAAGA AATACATGGC TATTCTGGAA 
GCGGCAGTGG GCATTACCCC ACTTAACAAA AGAGAGCTTC TACCCGAGAG CCGGAGACAC 
GTAAACCTTT GGCAGCACCC GGGGGCCCGG AGTCCTGCCT TGCTCTCTTC TCCCTGTCAG 
TGGGACTGGC ACCCAGGATA CAGCTCCAGG CCCAAGCACT CTGCAGCGCA CAGACCGGTG 
TCCACCTACA GCCTGCTCTC CCAGGGCCCA GGCATGGAGG AGGTGATATG GGAACAGTAC 
ACTGTGACCC TGCAGAAGGA TTCCAAAAGA GGATTTGGAA TCGCAGTGTC AGGAGGCAGA 
GACAACCCCC ACTTTGAAAA TGGGGAGACG TCAATCGTCA TTTCAGATGT ACTCCCAGGT 
GGACCCGCTG ATGGCTTGCT TCAAGAAAAT GACAGGGTGG TCATGGTCAA TGGCACCCCC 
ATGGAGGATG TGCTCCATTC ATTTGCCGTT CAGCAGCTTA GGAAAAGTGG GAAGATCGCA 
GCCATTGTGG TCAAGAGGCC TCGGAAGGTT CAGGTGGCCC CGCTGCAGGG CAGCCCCCCT 
CTCAGTCATG ATGACCGAGG TTTTGAGATG ATGGATGAAT TTGATGGCAG AAGTTTCCGC 
AGCGGCTACA GTGAGAGGAG CCGGCACAGC AGCCACGATA TGCTCAGCCA CAGCTGGGAG 
GACAGCAGGG AGAGGGGGCG TCCCCATCAG CGCGCACAGA GCCGGGAGCG GGAGCGCAGC 
CGTGGCCGGA GCCTGGAACG TGGCCTGGAC CATGAAGACT ACGGGCGCAG CCATGAGCGC 
AGCCGCGGCC GGAGCCTGGA GCGCGGTCTA GACCGCGACT TTGTGTCCCG GGACTACAGC 
CGTGGACGGA GCATCGACAG GGACTACGAC CGAGACTATG AGCGCTCCTA TCACCAAGCC 
TATGAGCCTG ACTACGAAGG CCGCTACAGC CCATCTTATG ACCGCAGAGC CCATCCAGAG 
ACCCGCTATG AACGGAGCCG CAGCCGAGAA CACCTACGGT CCCGAAGCCC CACCCCCGAG 
TCTAGGTCCC GGCAGGAACA CAAGGGCCAG CATGATCCCG ACAGGCCCAT CGGGGTCCTT 
CTGACCAAAA GCAAAGCAAA CGAAGAGTAC GGGCTCCGGC TGGGAAGTCA GATCTTCATT 
AAGGAAATGA CCAGAACAGG ACTGGCAACA AAAGATGGCA ACCTTCATGA GGGGGACATA 
ATTCTCAAGA TCAATGGAAC TGTTACTGAG AACATGTCTT TAACCGATGC TCGGAAGTTA 
ATAGAAAAGT CTCGAGGAAA ACTGCAGCTG GTGGTGTTGA GAGACAGCAA GCAGACCCTC 
ATCAACATCC CATCTCTGAA TGACAGCGAC TCGGAAGTGG AGGATATCTC GGAAATCGAG 
TCCAACCGAT CTTTTTCTCC AGAGGAGAGG CGCCAGCAGT ATTCTGATCA GGAGTATCAT 
TCCTCCACTG AGAAGCTGAA GGAGAGGCCA AGCTCAAGAG AGGAGACCTC AGGCAGAATG 
TCCAGGATGG GTGCCACACC CACGCCGTTC AAGTCCACGG GGGACATCAC AGCTGCAGGT 
GTCACAGAGG CCAACAAGGA GCCCAGGTCC CAGGAGGAAT CCCCAGTTCC TCAACCCAGA 
ACAGCATCAA GAGTCTTTCT TCGTCCTAGT CCCGAAGATG AAGCAATATA TGGCCCTAAC 
ACCAAAATGG TGAAGTTCAA GAAGGGAGAC AGCGTGGGCC TCCGGTTGGC TGGTGGAAAT 
GATGTTGGCA TATTTGTGGC TGGCATTCAG GAGGGCACCT CTGCAGAGCA GGAGGGCCTA 
CAAGAAGGGG ACCAGATTCT GAAGGTGAAC ACACAAGATT TCAGAGGGCT GGTCCGGGAA 
GATGCCGTCC TCTACCTGTT AGAAATCCCT AAAGGTGAAA CCGTGACCAT TTTGGCTCAG 
AGCCGAGCAG ACGTGTATAG AGACATCCTG GCCTGTGGCA GGGGAGACTC GTTCTTCATA 
AGGAGCCACT TTGAATGTGA GAAGGAGACT CCGCAGAGCT TGGCCTTCAC CAGGGGAGAG 
GTCTTCCGAG TGGTAGACAC GCTGTACGAT GGCAAACTGG GCCACTGGCT GGCTGTGAGG 
ATTGGAAATG AGCTGGAGAA GGGCTTAATC CCTAACAAAA GCAGAGCTGA GCAGATGGCC 
AGTGTCCAGA ATGCCCAGCG AGAGAATGCT GGGGACAGAG CAGACTTCTG GCGGATGCGT 
GGCCAGAGAT CCGGGGTCAA GAAGAACATT CGCAAGAGCC GGGAAGACCT GGCAGCTGCT 
GTGTCGGTTA GCACCAAGTT CCCCGCCTAC GAGAAGGTTC TGCTTCGGGA AGCTGGCTTC 
AAGAGACCCG TGGTTCTGTT TGGCCCCATA GCAGATATAG CAATGGAAAG GCTGACTACT 
GAGCTACCCG ACCTGTTTCA AACTGCAAAA ACAGAACCCA AAGATGCGGG ATCTGAGAAA 
TCCAGTGGAG TGGTTCGGTT GAATACTGTG AGGCAAATTA TTGAGCAGGA CAAGCATGCC 
CTGCTCGACG TTACCCCCAA AGCTGTGGAC CTGCTCAATT ATACTCAGTG GTTCCCAATC 
GTGATTTTCT TCAACCCGGA TTCCAGACAA GGCATTAAAA CCATAAGGCA GAGGTTGAAC 
CCAACATCCA ATAAAAGTTC TCGCAAGTTA TTCGATCAAG CCAACAAGCT CAAAAAAACC 
TGTTCTCATC TTTTTACAGC TACCATCAAC GTGAATTCAG CCAATGATGG CTGGTTTGGC 
AGCCTGAAGG ACAGCATTCA GCAGCAGCAA AACGAAGCAG TGTGGGTCTC TGAAGGAAAG 
ATGGAGGGGA TGGATGATGA CGCTGAAGAC CGCATGTCCT ACTTAACCGC CATGGGTGCG 
GACTATCTGA GTTGTGACAG CCGTCTCATC AGTGACTTTG AAGATACGGA CGGCGAGGGA 
GGCGCCTACA CTGACAATGA GCTGGATGAG CCAGCTGAGG AGCCGCTGGT GTCTTCCATC 
ACCCGCTCCT CAGAGCCGGT GCAGCATGAG GAGGTCAGAA ACCAAAACAG AGAGGACTCT 
TTCAACTACT CCAAGTCAAA CCTCTCTGCC ATGGCTGGCA GTGAAATCCC GGGGGGATCC 
ACCAAAGGGT GTCCTCCCCC TATTGCGGTG AAACCTGCCT TTGGGCGATC CATCCTGAAG 
CCTTCTACTC CAGTCCCCAT GCCTGAGAGT GAGGAGGTTG GGGAGAGCAC CGAGGAGCAG 
GAAGAGGCTC CCAAATCAGT CCTGGGCAGA GTGAAAATCT TCGAGAAGAT GGACCACAAG 
GCGAAATTAC AGAGGATGCA GGAGCTCCAA GAAGCACAGA ATGCGAGGAT TGAAATAGCT 
CAGAAGCATC CTGACATCTA TGCGGTTCCA ATCAAAGCCC CCAAGCCAGA TGCTGGCCTG 
CCCCAGCACA TGAGTTCTAG ACCCCCAGAG CCACAGAAAG CTCCTTCTAG GCTTTACCAG 
GACACCAGAG GAAGCTACGG CAGTGATCCC GAGGAAGAGG AGGAGTACCG CCAACAGTTG 
GCAGCACACT CGAAGCGTGG TTACTACAGC CAGCCCTCCC GGTACCGAGA CACCGAATTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp2 ( XM_006231177.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231239.1 Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X3, mRNA.

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CloneID	ORa23973
Clone ID Related Accession (Same CDS sequence)	XM_006231177.3
Accession Version	XM_006231177.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tight junction protein ZO-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231177.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGCTG CTCAGGTCTT CCAGAGGATG TGGATCCAGG CAGTTAAAAA GTTGGAGAGA 
TGGAAAGGCC ATGGCCCAGG CATGGAGGAG GTGATATGGG AACAGTACAC TGTGACCCTG 
CAGAAGGATT CCAAAAGAGG ATTTGGAATC GCAGTGTCAG GAGGCAGAGA CAACCCCCAC 
TTTGAAAATG GGGAGACGTC AATCGTCATT TCAGATGTAC TCCCAGGTGG ACCCGCTGAT 
GGCTTGCTTC AAGAAAATGA CAGGGTGGTC ATGGTCAATG GCACCCCCAT GGAGGATGTG 
CTCCATTCAT TTGCCGTTCA GCAGCTTAGG AAAAGTGGGA AGATCGCAGC CATTGTGGTC 
AAGAGGCCTC GGAAGGTTCA GGTGGCCCCG CTGCAGGGCA GCCCCCCTCT CAGTCATGAT 
GACCGAGGTT TTGAGATGAT GGATGAATTT GATGGCAGAA GTTTCCGCAG CGGCTACAGT 
GAGAGGAGCC GGCACAGCAG CCACGATATG CTCAGCCACA GCTGGGAGGA CAGCAGGGAG 
AGGGGGCGTC CCCATCAGCG CGCACAGAGC CGGGAGCGGG AGCGCAGCCG TGGCCGGAGC 
CTGGAACGTG GCCTGGACCA TGAAGACTAC GGGCGCAGCC ATGAGCGCAG CCGCGGCCGG 
AGCCTGGAGC GCGGTCTAGA CCGCGACTTT GTGTCCCGGG ACTACAGCCG TGGACGGAGC 
ATCGACAGGG ACTACGACCG AGACTATGAG CGCTCCTATC ACCAAGCCTA TGAGCCTGAC 
TACGAAGGCC GCTACAGCCC ATCTTATGAC CGCAGAGCCC ATCCAGAGAC CCGCTATGAA 
CGGAGCCGCA GCCGAGAACA CCTACGGTCC CGAAGCCCCA CCCCCGAGTC TAGGTCCCGG 
CAGGAACACA AGGGCCAGCA TGATCCCGAC AGGCCCATCG GGGTCCTTCT GACCAAAAGC 
AAAGCAAACG AAGAGTACGG GCTCCGGCTG GGAAGTCAGA TCTTCATTAA GGAAATGACC 
AGAACAGGAC TGGCAACAAA AGATGGCAAC CTTCATGAGG GGGACATAAT TCTCAAGATC 
AATGGAACTG TTACTGAGAA CATGTCTTTA ACCGATGCTC GGAAGTTAAT AGAAAAGTCT 
CGAGGAAAAC TGCAGCTGGT GGTGTTGAGA GACAGCAAGC AGACCCTCAT CAACATCCCA 
TCTCTGAATG ACAGCGACTC GGAAGTGGAG GATATCTCGG AAATCGAGTC CAACCGATCT 
TTTTCTCCAG AGGAGAGGCG CCAGCAGTAT TCTGATCAGG AGTATCATTC CTCCACTGAG 
AAGCTGAAGG AGAGGCCAAG CTCAAGAGAG GAGACCTCAG GCAGAATGTC CAGGATGGGT 
GCCACACCCA CGCCGTTCAA GTCCACGGGG GACATCACAG CTGCAGGTGT CACAGAGGCC 
AACAAGGAGC CCAGGTCCCA GGAGGAATCC CCAGTTCCTC AACCCAGAAC AGCATCAAGA 
GTCTTTCTTC GTCCTAGTCC CGAAGATGAA GCAATATATG GCCCTAACAC CAAAATGGTG 
AAGTTCAAGA AGGGAGACAG CGTGGGCCTC CGGTTGGCTG GTGGAAATGA TGTTGGCATA 
TTTGTGGCTG GCATTCAGGA GGGCACCTCT GCAGAGCAGG AGGGCCTACA AGAAGGGGAC 
CAGATTCTGA AGGTGAACAC ACAAGATTTC AGAGGGCTGG TCCGGGAAGA TGCCGTCCTC 
TACCTGTTAG AAATCCCTAA AGGTGAAACC GTGACCATTT TGGCTCAGAG CCGAGCAGAC 
GTGTATAGAG ACATCCTGGC CTGTGGCAGG GGAGACTCGT TCTTCATAAG GAGCCACTTT 
GAATGTGAGA AGGAGACTCC GCAGAGCTTG GCCTTCACCA GGGGAGAGGT CTTCCGAGTG 
GTAGACACGC TGTACGATGG CAAACTGGGC CACTGGCTGG CTGTGAGGAT TGGAAATGAG 
CTGGAGAAGG GCTTAATCCC TAACAAAAGC AGAGCTGAGC AGATGGCCAG TGTCCAGAAT 
GCCCAGCGAG AGAATGCTGG GGACAGAGCA GACTTCTGGC GGATGCGTGG CCAGAGATCC 
GGGGTCAAGA AGAACATTCG CAAGAGCCGG GAAGACCTGG CAGCTGCTGT GTCGGTTAGC 
ACCAAGTTCC CCGCCTACGA GAAGGTTCTG CTTCGGGAAG CTGGCTTCAA GAGACCCGTG 
GTTCTGTTTG GCCCCATAGC AGATATAGCA ATGGAAAGGC TGACTACTGA GCTACCCGAC 
CTGTTTCAAA CTGCAAAAAC AGAACCCAAA GATGCGGGAT CTGAGAAATC CAGTGGAGTG 
GTTCGGTTGA ATACTGTGAG GCAAATTATT GAGCAGGACA AGCATGCCCT GCTCGACGTT 
ACCCCCAAAG CTGTGGACCT GCTCAATTAT ACTCAGTGGT TCCCAATCGT GATTTTCTTC 
AACCCGGATT CCAGACAAGG CATTAAAACC ATAAGGCAGA GGTTGAACCC AACATCCAAT 
AAAAGTTCTC GCAAGTTATT CGATCAAGCC AACAAGCTCA AAAAAACCTG TTCTCATCTT 
TTTACAGCTA CCATCAACGT GAATTCAGCC AATGATGGCT GGTTTGGCAG CCTGAAGGAC 
AGCATTCAGC AGCAGCAAAA CGAAGCAGTG TGGGTCTCTG AAGGAAAGAT GGAGGGGATG 
GATGATGACG CTGAAGACCG CATGTCCTAC TTAACCGCCA TGGGTGCGGA CTATCTGAGT 
TGTGACAGCC GTCTCATCAG TGACTTTGAA GATACGGACG GCGAGGGAGG CGCCTACACT 
GACAATGAGC TGGATGAGCC AGCTGAGGAG CCGCTGGTGT CTTCCATCAC CCGCTCCTCA 
GAGCCGGTGC AGCATGAGGA GAGCATAAGG AAGCCCAGCC CAGAGCCACG CGCTCAGATG 
AGGAGGGCAG CTAGCAGAGA CCAGCTTAGG GATGGTAGCC CGCCCCCAGC ATTCAAGCCA 
GAGCCGCCCA AGGTCAGAAA CCAAAACAGA GAGGACTCTT TCAACTACTC CAAGTCAAAC 
CTCTCTGCCA TGGCTGGCAG TGAAATCCCG GGGGGATCCA CCAAAGGGTG TCCTCCCCCT 
ATTGCGGTGA AACCTGCCTT TGGGCGATCC ATCCTGAAGC CTTCTACTCC AGTCCCCATG 
CCTGAGAGTG AGGAGGTTGG GGAGAGCACC GAGGAGCAGG AAGAGGCTCC CAAATCAGTC 
CTGGGCAGAG TGAAAATCTT CGAGAAGATG GACCACAAGG CGAAATTACA GAGGATGCAG 
GAGCTCCAAG AAGCACAGAA TGCGAGGATT GAAATAGCTC AGAAGCATCC TGACATCTAT 
GCGGTTCCAA TCAAAGCCCC CAAGCCAGAT GCTGGCCTGC CCCAGCACAT GAGTTCTAGA 
CCCCCAGAGC CACAGAAAGC TCCTTCTAGG CTTTACCAGG ACACCAGAGG AAGCTACGGC 
AGTGATCCCG AGGAAGAGGA GGAGTACCGC CAACAGTTGG CAGCACACTC GAAGCGTGGT 
TACTACAGCC AGCCCTCCCG GTACCGAGAC ACCGAATTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231239.1
CDS	539..4120
Translation	MRAAQVFQRMWIQAVKKLERWKGHGPGMEEVIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKIAAIVVKRPRKVQVAPLQGSPPLSHDDRGFEMMDEFDGRSFRSGYSERSRHSSHDMLSHSWEDSRERGRPHQRAQSRERERSRGRSLERGLDHEDYGRSHERSRGRSLERGLDRDFVSRDYSRGRSIDRDYDRDYERSYHQAYEPDYEGRYSPSYDRRAHPETRYERSRSREHLRSRSPTPESRSRQEHKGQHDPDRPIGVLLTKSKANEEYGLRLGSQIFIKEMTRTGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSKQTLINIPSLNDSDSEVEDISEIESNRSFSPEERRQQYSDQEYHSSTEKLKERPSSREETSGRMSRMGATPTPFKSTGDITAAGVTEANKEPRSQEESPVPQPRTASRVFLRPSPEDEAIYGPNTKMVKFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGLVREDAVLYLLEIPKGETVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEVFRVVDTLYDGKLGHWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRENAGDRADFWRMRGQRSGVKKNIRKSREDLAAAVSVSTKFPAYEKVLLREAGFKRPVVLFGPIADIAMERLTTELPDLFQTAKTEPKDAGSEKSSGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGIKTIRQRLNPTSNKSSRKLFDQANKLKKTCSHLFTATINVNSANDGWFGSLKDSIQQQQNEAVWVSEGKMEGMDDDAEDRMSYLTAMGADYLSCDSRLISDFEDTDGEGGAYTDNELDEPAEEPLVSSITRSSEPVQHEESIRKPSPEPRAQMRRAASRDQLRDGSPPPAFKPEPPKVRNQNREDSFNYSKSNLSAMAGSEIPGGSTKGCPPPIAVKPAFGRSILKPSTPVPMPESEEVGESTEEQEEAPKSVLGRVKIFEKMDHKAKLQRMQELQEAQNARIEIAQKHPDIYAVPIKAPKPDAGLPQHMSSRPPEPQKAPSRLYQDTRGSYGSDPEEEEEYRQQLAAHSKRGYYSQPSRYRDTEL

Target ORF information:

RefSeq Version	XM_006231177.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231177.3

ORF Insert Sequence:

ATGAGGGCTG CTCAGGTCTT CCAGAGGATG TGGATCCAGG CAGTTAAAAA GTTGGAGAGA 
TGGAAAGGCC ATGGCCCAGG CATGGAGGAG GTGATATGGG AACAGTACAC TGTGACCCTG 
CAGAAGGATT CCAAAAGAGG ATTTGGAATC GCAGTGTCAG GAGGCAGAGA CAACCCCCAC 
TTTGAAAATG GGGAGACGTC AATCGTCATT TCAGATGTAC TCCCAGGTGG ACCCGCTGAT 
GGCTTGCTTC AAGAAAATGA CAGGGTGGTC ATGGTCAATG GCACCCCCAT GGAGGATGTG 
CTCCATTCAT TTGCCGTTCA GCAGCTTAGG AAAAGTGGGA AGATCGCAGC CATTGTGGTC 
AAGAGGCCTC GGAAGGTTCA GGTGGCCCCG CTGCAGGGCA GCCCCCCTCT CAGTCATGAT 
GACCGAGGTT TTGAGATGAT GGATGAATTT GATGGCAGAA GTTTCCGCAG CGGCTACAGT 
GAGAGGAGCC GGCACAGCAG CCACGATATG CTCAGCCACA GCTGGGAGGA CAGCAGGGAG 
AGGGGGCGTC CCCATCAGCG CGCACAGAGC CGGGAGCGGG AGCGCAGCCG TGGCCGGAGC 
CTGGAACGTG GCCTGGACCA TGAAGACTAC GGGCGCAGCC ATGAGCGCAG CCGCGGCCGG 
AGCCTGGAGC GCGGTCTAGA CCGCGACTTT GTGTCCCGGG ACTACAGCCG TGGACGGAGC 
ATCGACAGGG ACTACGACCG AGACTATGAG CGCTCCTATC ACCAAGCCTA TGAGCCTGAC 
TACGAAGGCC GCTACAGCCC ATCTTATGAC CGCAGAGCCC ATCCAGAGAC CCGCTATGAA 
CGGAGCCGCA GCCGAGAACA CCTACGGTCC CGAAGCCCCA CCCCCGAGTC TAGGTCCCGG 
CAGGAACACA AGGGCCAGCA TGATCCCGAC AGGCCCATCG GGGTCCTTCT GACCAAAAGC 
AAAGCAAACG AAGAGTACGG GCTCCGGCTG GGAAGTCAGA TCTTCATTAA GGAAATGACC 
AGAACAGGAC TGGCAACAAA AGATGGCAAC CTTCATGAGG GGGACATAAT TCTCAAGATC 
AATGGAACTG TTACTGAGAA CATGTCTTTA ACCGATGCTC GGAAGTTAAT AGAAAAGTCT 
CGAGGAAAAC TGCAGCTGGT GGTGTTGAGA GACAGCAAGC AGACCCTCAT CAACATCCCA 
TCTCTGAATG ACAGCGACTC GGAAGTGGAG GATATCTCGG AAATCGAGTC CAACCGATCT 
TTTTCTCCAG AGGAGAGGCG CCAGCAGTAT TCTGATCAGG AGTATCATTC CTCCACTGAG 
AAGCTGAAGG AGAGGCCAAG CTCAAGAGAG GAGACCTCAG GCAGAATGTC CAGGATGGGT 
GCCACACCCA CGCCGTTCAA GTCCACGGGG GACATCACAG CTGCAGGTGT CACAGAGGCC 
AACAAGGAGC CCAGGTCCCA GGAGGAATCC CCAGTTCCTC AACCCAGAAC AGCATCAAGA 
GTCTTTCTTC GTCCTAGTCC CGAAGATGAA GCAATATATG GCCCTAACAC CAAAATGGTG 
AAGTTCAAGA AGGGAGACAG CGTGGGCCTC CGGTTGGCTG GTGGAAATGA TGTTGGCATA 
TTTGTGGCTG GCATTCAGGA GGGCACCTCT GCAGAGCAGG AGGGCCTACA AGAAGGGGAC 
CAGATTCTGA AGGTGAACAC ACAAGATTTC AGAGGGCTGG TCCGGGAAGA TGCCGTCCTC 
TACCTGTTAG AAATCCCTAA AGGTGAAACC GTGACCATTT TGGCTCAGAG CCGAGCAGAC 
GTGTATAGAG ACATCCTGGC CTGTGGCAGG GGAGACTCGT TCTTCATAAG GAGCCACTTT 
GAATGTGAGA AGGAGACTCC GCAGAGCTTG GCCTTCACCA GGGGAGAGGT CTTCCGAGTG 
GTAGACACGC TGTACGATGG CAAACTGGGC CACTGGCTGG CTGTGAGGAT TGGAAATGAG 
CTGGAGAAGG GCTTAATCCC TAACAAAAGC AGAGCTGAGC AGATGGCCAG TGTCCAGAAT 
GCCCAGCGAG AGAATGCTGG GGACAGAGCA GACTTCTGGC GGATGCGTGG CCAGAGATCC 
GGGGTCAAGA AGAACATTCG CAAGAGCCGG GAAGACCTGG CAGCTGCTGT GTCGGTTAGC 
ACCAAGTTCC CCGCCTACGA GAAGGTTCTG CTTCGGGAAG CTGGCTTCAA GAGACCCGTG 
GTTCTGTTTG GCCCCATAGC AGATATAGCA ATGGAAAGGC TGACTACTGA GCTACCCGAC 
CTGTTTCAAA CTGCAAAAAC AGAACCCAAA GATGCGGGAT CTGAGAAATC CAGTGGAGTG 
GTTCGGTTGA ATACTGTGAG GCAAATTATT GAGCAGGACA AGCATGCCCT GCTCGACGTT 
ACCCCCAAAG CTGTGGACCT GCTCAATTAT ACTCAGTGGT TCCCAATCGT GATTTTCTTC 
AACCCGGATT CCAGACAAGG CATTAAAACC ATAAGGCAGA GGTTGAACCC AACATCCAAT 
AAAAGTTCTC GCAAGTTATT CGATCAAGCC AACAAGCTCA AAAAAACCTG TTCTCATCTT 
TTTACAGCTA CCATCAACGT GAATTCAGCC AATGATGGCT GGTTTGGCAG CCTGAAGGAC 
AGCATTCAGC AGCAGCAAAA CGAAGCAGTG TGGGTCTCTG AAGGAAAGAT GGAGGGGATG 
GATGATGACG CTGAAGACCG CATGTCCTAC TTAACCGCCA TGGGTGCGGA CTATCTGAGT 
TGTGACAGCC GTCTCATCAG TGACTTTGAA GATACGGACG GCGAGGGAGG CGCCTACACT 
GACAATGAGC TGGATGAGCC AGCTGAGGAG CCGCTGGTGT CTTCCATCAC CCGCTCCTCA 
GAGCCGGTGC AGCATGAGGA GAGCATAAGG AAGCCCAGCC CAGAGCCACG CGCTCAGATG 
AGGAGGGCAG CTAGCAGAGA CCAGCTTAGG GATGGTAGCC CGCCCCCAGC ATTCAAGCCA 
GAGCCGCCCA AGGTCAGAAA CCAAAACAGA GAGGACTCTT TCAACTACTC CAAGTCAAAC 
CTCTCTGCCA TGGCTGGCAG TGAAATCCCG GGGGGATCCA CCAAAGGGTG TCCTCCCCCT 
ATTGCGGTGA AACCTGCCTT TGGGCGATCC ATCCTGAAGC CTTCTACTCC AGTCCCCATG 
CCTGAGAGTG AGGAGGTTGG GGAGAGCACC GAGGAGCAGG AAGAGGCTCC CAAATCAGTC 
CTGGGCAGAG TGAAAATCTT CGAGAAGATG GACCACAAGG CGAAATTACA GAGGATGCAG 
GAGCTCCAAG AAGCACAGAA TGCGAGGATT GAAATAGCTC AGAAGCATCC TGACATCTAT 
GCGGTTCCAA TCAAAGCCCC CAAGCCAGAT GCTGGCCTGC CCCAGCACAT GAGTTCTAGA 
CCCCCAGAGC CACAGAAAGC TCCTTCTAGG CTTTACCAGG ACACCAGAGG AAGCTACGGC 
AGTGATCCCG AGGAAGAGGA GGAGTACCGC CAACAGTTGG CAGCACACTC GAAGCGTGGT 
TACTACAGCC AGCCCTCCCG GTACCGAGAC ACCGAATTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp2 ( XM_006231178.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231240.1 Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa23974
Clone ID Related Accession (Same CDS sequence)	XM_006231178.3
Accession Version	XM_006231178.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tight junction protein ZO-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231178.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGC GGGGAGACCG CGGGTTCCCA CTGCGGCGGG AGCTGTCGGG CTGGCAGCGC 
GGCCCAGGCA TGGAGGAGGT GATATGGGAA CAGTACACTG TGACCCTGCA GAAGGATTCC 
AAAAGAGGAT TTGGAATCGC AGTGTCAGGA GGCAGAGACA ACCCCCACTT TGAAAATGGG 
GAGACGTCAA TCGTCATTTC AGATGTACTC CCAGGTGGAC CCGCTGATGG CTTGCTTCAA 
GAAAATGACA GGGTGGTCAT GGTCAATGGC ACCCCCATGG AGGATGTGCT CCATTCATTT 
GCCGTTCAGC AGCTTAGGAA AAGTGGGAAG ATCGCAGCCA TTGTGGTCAA GAGGCCTCGG 
AAGGTTCAGG TGGCCCCGCT GCAGGGCAGC CCCCCTCTCA GTCATGATGA CCGAGGTTTT 
GAGATGATGG ATGAATTTGA TGGCAGAAGT TTCCGCAGCG GCTACAGTGA GAGGAGCCGG 
CACAGCAGCC ACGATATGCT CAGCCACAGC TGGGAGGACA GCAGGGAGAG GGGGCGTCCC 
CATCAGCGCG CACAGAGCCG GGAGCGGGAG CGCAGCCGTG GCCGGAGCCT GGAACGTGGC 
CTGGACCATG AAGACTACGG GCGCAGCCAT GAGCGCAGCC GCGGCCGGAG CCTGGAGCGC 
GGTCTAGACC GCGACTTTGT GTCCCGGGAC TACAGCCGTG GACGGAGCAT CGACAGGGAC 
TACGACCGAG ACTATGAGCG CTCCTATCAC CAAGCCTATG AGCCTGACTA CGAAGGCCGC 
TACAGCCCAT CTTATGACCG CAGAGCCCAT CCAGAGACCC GCTATGAACG GAGCCGCAGC 
CGAGAACACC TACGGTCCCG AAGCCCCACC CCCGAGTCTA GGTCCCGGCA GGAACACAAG 
GGCCAGCATG ATCCCGACAG GCCCATCGGG GTCCTTCTGA CCAAAAGCAA AGCAAACGAA 
GAGTACGGGC TCCGGCTGGG AAGTCAGATC TTCATTAAGG AAATGACCAG AACAGGACTG 
GCAACAAAAG ATGGCAACCT TCATGAGGGG GACATAATTC TCAAGATCAA TGGAACTGTT 
ACTGAGAACA TGTCTTTAAC CGATGCTCGG AAGTTAATAG AAAAGTCTCG AGGAAAACTG 
CAGCTGGTGG TGTTGAGAGA CAGCAAGCAG ACCCTCATCA ACATCCCATC TCTGAATGAC 
AGCGACTCGG AAGTGGAGGA TATCTCGGAA ATCGAGTCCA ACCGATCTTT TTCTCCAGAG 
GAGAGGCGCC AGCAGTATTC TGATCAGGAG TATCATTCCT CCACTGAGAA GCTGAAGGAG 
AGGCCAAGCT CAAGAGAGGA GACCTCAGGC AGAATGTCCA GGATGGGTGC CACACCCACG 
CCGTTCAAGT CCACGGGGGA CATCACAGCT GCAGGTGTCA CAGAGGCCAA CAAGGAGCCC 
AGGTCCCAGG AGGAATCCCC AGTTCCTCAA CCCAGAACAG CATCAAGAGT CTTTCTTCGT 
CCTAGTCCCG AAGATGAAGC AATATATGGC CCTAACACCA AAATGGTGAA GTTCAAGAAG 
GGAGACAGCG TGGGCCTCCG GTTGGCTGGT GGAAATGATG TTGGCATATT TGTGGCTGGC 
ATTCAGGAGG GCACCTCTGC AGAGCAGGAG GGCCTACAAG AAGGGGACCA GATTCTGAAG 
GTGAACACAC AAGATTTCAG AGGGCTGGTC CGGGAAGATG CCGTCCTCTA CCTGTTAGAA 
ATCCCTAAAG GTGAAACCGT GACCATTTTG GCTCAGAGCC GAGCAGACGT GTATAGAGAC 
ATCCTGGCCT GTGGCAGGGG AGACTCGTTC TTCATAAGGA GCCACTTTGA ATGTGAGAAG 
GAGACTCCGC AGAGCTTGGC CTTCACCAGG GGAGAGGTCT TCCGAGTGGT AGACACGCTG 
TACGATGGCA AACTGGGCCA CTGGCTGGCT GTGAGGATTG GAAATGAGCT GGAGAAGGGC 
TTAATCCCTA ACAAAAGCAG AGCTGAGCAG ATGGCCAGTG TCCAGAATGC CCAGCGAGAG 
AATGCTGGGG ACAGAGCAGA CTTCTGGCGG ATGCGTGGCC AGAGATCCGG GGTCAAGAAG 
AACATTCGCA AGAGCCGGGA AGACCTGGCA GCTGCTGTGT CGGTTAGCAC CAAGTTCCCC 
GCCTACGAGA AGGTTCTGCT TCGGGAAGCT GGCTTCAAGA GACCCGTGGT TCTGTTTGGC 
CCCATAGCAG ATATAGCAAT GGAAAGGCTG ACTACTGAGC TACCCGACCT GTTTCAAACT 
GCAAAAACAG AACCCAAAGA TGCGGGATCT GAGAAATCCA GTGGAGTGGT TCGGTTGAAT 
ACTGTGAGGC AAATTATTGA GCAGGACAAG CATGCCCTGC TCGACGTTAC CCCCAAAGCT 
GTGGACCTGC TCAATTATAC TCAGTGGTTC CCAATCGTGA TTTTCTTCAA CCCGGATTCC 
AGACAAGGCA TTAAAACCAT AAGGCAGAGG TTGAACCCAA CATCCAATAA AAGTTCTCGC 
AAGTTATTCG ATCAAGCCAA CAAGCTCAAA AAAACCTGTT CTCATCTTTT TACAGCTACC 
ATCAACGTGA ATTCAGCCAA TGATGGCTGG TTTGGCAGCC TGAAGGACAG CATTCAGCAG 
CAGCAAAACG AAGCAGTGTG GGTCTCTGAA GGAAAGATGG AGGGGATGGA TGATGACGCT 
GAAGACCGCA TGTCCTACTT AACCGCCATG GGTGCGGACT ATCTGAGTTG TGACAGCCGT 
CTCATCAGTG ACTTTGAAGA TACGGACGGC GAGGGAGGCG CCTACACTGA CAATGAGCTG 
GATGAGCCAG CTGAGGAGCC GCTGGTGTCT TCCATCACCC GCTCCTCAGA GCCGGTGCAG 
CATGAGGAGA GCATAAGGAA GCCCAGCCCA GAGCCACGCG CTCAGATGAG GAGGGCAGCT 
AGCAGAGACC AGCTTAGGGA TGGTAGCCCG CCCCCAGCAT TCAAGCCAGA GCCGCCCAAG 
GTCAGAAACC AAAACAGAGA GGACTCTTTC AACTACTCCA AGTCAAACCT CTCTGCCATG 
GCTGGCAGTG AAATCCCGGG GGGATCCACC AAAGGGTGTC CTCCCCCTAT TGCGGTGAAA 
CCTGCCTTTG GGCGATCCAT CCTGAAGCCT TCTACTCCAG TCCCCATGCC TGAGAGTGAG 
GAGGTTGGGG AGAGCACCGA GGAGCAGGAA GAGGCTCCCA AATCAGTCCT GGGCAGAGTG 
AAAATCTTCG AGAAGATGGA CCACAAGGCG AAATTACAGA GGATGCAGGA GCTCCAAGAA 
GCACAGAATG CGAGGATTGA AATAGCTCAG AAGCATCCTG ACATCTATGC GGTTCCAATC 
AAAGCCCCCA AGCCAGATGC TGGCCTGCCC CAGCACATGA GTTCTAGACC CCCAGAGCCA 
CAGAAAGCTC CTTCTAGGCT TTACCAGGAC ACCAGAGGAA GCTACGGCAG TGATCCCGAG 
GAAGAGGAGG AGTACCGCCA ACAGTTGGCA GCACACTCGA AGCGTGGTTA CTACAGCCAG 
CCCTCCCGGT ACCGAGACAC CGAATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231240.1
CDS	2363..5932
Translation	MPVRGDRGFPLRRELSGWQRGPGMEEVIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKIAAIVVKRPRKVQVAPLQGSPPLSHDDRGFEMMDEFDGRSFRSGYSERSRHSSHDMLSHSWEDSRERGRPHQRAQSRERERSRGRSLERGLDHEDYGRSHERSRGRSLERGLDRDFVSRDYSRGRSIDRDYDRDYERSYHQAYEPDYEGRYSPSYDRRAHPETRYERSRSREHLRSRSPTPESRSRQEHKGQHDPDRPIGVLLTKSKANEEYGLRLGSQIFIKEMTRTGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSKQTLINIPSLNDSDSEVEDISEIESNRSFSPEERRQQYSDQEYHSSTEKLKERPSSREETSGRMSRMGATPTPFKSTGDITAAGVTEANKEPRSQEESPVPQPRTASRVFLRPSPEDEAIYGPNTKMVKFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGLVREDAVLYLLEIPKGETVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEVFRVVDTLYDGKLGHWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRENAGDRADFWRMRGQRSGVKKNIRKSREDLAAAVSVSTKFPAYEKVLLREAGFKRPVVLFGPIADIAMERLTTELPDLFQTAKTEPKDAGSEKSSGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGIKTIRQRLNPTSNKSSRKLFDQANKLKKTCSHLFTATINVNSANDGWFGSLKDSIQQQQNEAVWVSEGKMEGMDDDAEDRMSYLTAMGADYLSCDSRLISDFEDTDGEGGAYTDNELDEPAEEPLVSSITRSSEPVQHEESIRKPSPEPRAQMRRAASRDQLRDGSPPPAFKPEPPKVRNQNREDSFNYSKSNLSAMAGSEIPGGSTKGCPPPIAVKPAFGRSILKPSTPVPMPESEEVGESTEEQEEAPKSVLGRVKIFEKMDHKAKLQRMQELQEAQNARIEIAQKHPDIYAVPIKAPKPDAGLPQHMSSRPPEPQKAPSRLYQDTRGSYGSDPEEEEEYRQQLAAHSKRGYYSQPSRYRDTEL

Target ORF information:

RefSeq Version	XM_006231178.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231178.3

ORF Insert Sequence:

ATGCCGGTGC GGGGAGACCG CGGGTTCCCA CTGCGGCGGG AGCTGTCGGG CTGGCAGCGC 
GGCCCAGGCA TGGAGGAGGT GATATGGGAA CAGTACACTG TGACCCTGCA GAAGGATTCC 
AAAAGAGGAT TTGGAATCGC AGTGTCAGGA GGCAGAGACA ACCCCCACTT TGAAAATGGG 
GAGACGTCAA TCGTCATTTC AGATGTACTC CCAGGTGGAC CCGCTGATGG CTTGCTTCAA 
GAAAATGACA GGGTGGTCAT GGTCAATGGC ACCCCCATGG AGGATGTGCT CCATTCATTT 
GCCGTTCAGC AGCTTAGGAA AAGTGGGAAG ATCGCAGCCA TTGTGGTCAA GAGGCCTCGG 
AAGGTTCAGG TGGCCCCGCT GCAGGGCAGC CCCCCTCTCA GTCATGATGA CCGAGGTTTT 
GAGATGATGG ATGAATTTGA TGGCAGAAGT TTCCGCAGCG GCTACAGTGA GAGGAGCCGG 
CACAGCAGCC ACGATATGCT CAGCCACAGC TGGGAGGACA GCAGGGAGAG GGGGCGTCCC 
CATCAGCGCG CACAGAGCCG GGAGCGGGAG CGCAGCCGTG GCCGGAGCCT GGAACGTGGC 
CTGGACCATG AAGACTACGG GCGCAGCCAT GAGCGCAGCC GCGGCCGGAG CCTGGAGCGC 
GGTCTAGACC GCGACTTTGT GTCCCGGGAC TACAGCCGTG GACGGAGCAT CGACAGGGAC 
TACGACCGAG ACTATGAGCG CTCCTATCAC CAAGCCTATG AGCCTGACTA CGAAGGCCGC 
TACAGCCCAT CTTATGACCG CAGAGCCCAT CCAGAGACCC GCTATGAACG GAGCCGCAGC 
CGAGAACACC TACGGTCCCG AAGCCCCACC CCCGAGTCTA GGTCCCGGCA GGAACACAAG 
GGCCAGCATG ATCCCGACAG GCCCATCGGG GTCCTTCTGA CCAAAAGCAA AGCAAACGAA 
GAGTACGGGC TCCGGCTGGG AAGTCAGATC TTCATTAAGG AAATGACCAG AACAGGACTG 
GCAACAAAAG ATGGCAACCT TCATGAGGGG GACATAATTC TCAAGATCAA TGGAACTGTT 
ACTGAGAACA TGTCTTTAAC CGATGCTCGG AAGTTAATAG AAAAGTCTCG AGGAAAACTG 
CAGCTGGTGG TGTTGAGAGA CAGCAAGCAG ACCCTCATCA ACATCCCATC TCTGAATGAC 
AGCGACTCGG AAGTGGAGGA TATCTCGGAA ATCGAGTCCA ACCGATCTTT TTCTCCAGAG 
GAGAGGCGCC AGCAGTATTC TGATCAGGAG TATCATTCCT CCACTGAGAA GCTGAAGGAG 
AGGCCAAGCT CAAGAGAGGA GACCTCAGGC AGAATGTCCA GGATGGGTGC CACACCCACG 
CCGTTCAAGT CCACGGGGGA CATCACAGCT GCAGGTGTCA CAGAGGCCAA CAAGGAGCCC 
AGGTCCCAGG AGGAATCCCC AGTTCCTCAA CCCAGAACAG CATCAAGAGT CTTTCTTCGT 
CCTAGTCCCG AAGATGAAGC AATATATGGC CCTAACACCA AAATGGTGAA GTTCAAGAAG 
GGAGACAGCG TGGGCCTCCG GTTGGCTGGT GGAAATGATG TTGGCATATT TGTGGCTGGC 
ATTCAGGAGG GCACCTCTGC AGAGCAGGAG GGCCTACAAG AAGGGGACCA GATTCTGAAG 
GTGAACACAC AAGATTTCAG AGGGCTGGTC CGGGAAGATG CCGTCCTCTA CCTGTTAGAA 
ATCCCTAAAG GTGAAACCGT GACCATTTTG GCTCAGAGCC GAGCAGACGT GTATAGAGAC 
ATCCTGGCCT GTGGCAGGGG AGACTCGTTC TTCATAAGGA GCCACTTTGA ATGTGAGAAG 
GAGACTCCGC AGAGCTTGGC CTTCACCAGG GGAGAGGTCT TCCGAGTGGT AGACACGCTG 
TACGATGGCA AACTGGGCCA CTGGCTGGCT GTGAGGATTG GAAATGAGCT GGAGAAGGGC 
TTAATCCCTA ACAAAAGCAG AGCTGAGCAG ATGGCCAGTG TCCAGAATGC CCAGCGAGAG 
AATGCTGGGG ACAGAGCAGA CTTCTGGCGG ATGCGTGGCC AGAGATCCGG GGTCAAGAAG 
AACATTCGCA AGAGCCGGGA AGACCTGGCA GCTGCTGTGT CGGTTAGCAC CAAGTTCCCC 
GCCTACGAGA AGGTTCTGCT TCGGGAAGCT GGCTTCAAGA GACCCGTGGT TCTGTTTGGC 
CCCATAGCAG ATATAGCAAT GGAAAGGCTG ACTACTGAGC TACCCGACCT GTTTCAAACT 
GCAAAAACAG AACCCAAAGA TGCGGGATCT GAGAAATCCA GTGGAGTGGT TCGGTTGAAT 
ACTGTGAGGC AAATTATTGA GCAGGACAAG CATGCCCTGC TCGACGTTAC CCCCAAAGCT 
GTGGACCTGC TCAATTATAC TCAGTGGTTC CCAATCGTGA TTTTCTTCAA CCCGGATTCC 
AGACAAGGCA TTAAAACCAT AAGGCAGAGG TTGAACCCAA CATCCAATAA AAGTTCTCGC 
AAGTTATTCG ATCAAGCCAA CAAGCTCAAA AAAACCTGTT CTCATCTTTT TACAGCTACC 
ATCAACGTGA ATTCAGCCAA TGATGGCTGG TTTGGCAGCC TGAAGGACAG CATTCAGCAG 
CAGCAAAACG AAGCAGTGTG GGTCTCTGAA GGAAAGATGG AGGGGATGGA TGATGACGCT 
GAAGACCGCA TGTCCTACTT AACCGCCATG GGTGCGGACT ATCTGAGTTG TGACAGCCGT 
CTCATCAGTG ACTTTGAAGA TACGGACGGC GAGGGAGGCG CCTACACTGA CAATGAGCTG 
GATGAGCCAG CTGAGGAGCC GCTGGTGTCT TCCATCACCC GCTCCTCAGA GCCGGTGCAG 
CATGAGGAGA GCATAAGGAA GCCCAGCCCA GAGCCACGCG CTCAGATGAG GAGGGCAGCT 
AGCAGAGACC AGCTTAGGGA TGGTAGCCCG CCCCCAGCAT TCAAGCCAGA GCCGCCCAAG 
GTCAGAAACC AAAACAGAGA GGACTCTTTC AACTACTCCA AGTCAAACCT CTCTGCCATG 
GCTGGCAGTG AAATCCCGGG GGGATCCACC AAAGGGTGTC CTCCCCCTAT TGCGGTGAAA 
CCTGCCTTTG GGCGATCCAT CCTGAAGCCT TCTACTCCAG TCCCCATGCC TGAGAGTGAG 
GAGGTTGGGG AGAGCACCGA GGAGCAGGAA GAGGCTCCCA AATCAGTCCT GGGCAGAGTG 
AAAATCTTCG AGAAGATGGA CCACAAGGCG AAATTACAGA GGATGCAGGA GCTCCAAGAA 
GCACAGAATG CGAGGATTGA AATAGCTCAG AAGCATCCTG ACATCTATGC GGTTCCAATC 
AAAGCCCCCA AGCCAGATGC TGGCCTGCCC CAGCACATGA GTTCTAGACC CCCAGAGCCA 
CAGAAAGCTC CTTCTAGGCT TTACCAGGAC ACCAGAGGAA GCTACGGCAG TGATCCCGAG 
GAAGAGGAGG AGTACCGCCA ACAGTTGGCA GCACACTCGA AGCGTGGTTA CTACAGCCAG 
CCCTCCCGGT ACCGAGACAC CGAATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp2 ( XM_006231181.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231243.1 Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X5, mRNA.

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CloneID	ORa23977
Clone ID Related Accession (Same CDS sequence)	XM_006231181.3 , XM_008760302.2
Accession Version	XM_006231181.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tight junction protein ZO-2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231181.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG TGATATGGGA ACAGTACACT GTGACCCTGC AGAAGGATTC CAAAAGAGGA 
TTTGGAATCG CAGTGTCAGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACGTCA 
ATCGTCATTT CAGATGTACT CCCAGGTGGA CCCGCTGATG GCTTGCTTCA AGAAAATGAC 
AGGGTGGTCA TGGTCAATGG CACCCCCATG GAGGATGTGC TCCATTCATT TGCCGTTCAG 
CAGCTTAGGA AAAGTGGGAA GATCGCAGCC ATTGTGGTCA AGAGGCCTCG GAAGGTTCAG 
GTGGCCCCGC TGCAGGGCAG CCCCCCTCTC AGTCATGATG ACCGAGGTTT TGAGATGATG 
GATGAATTTG ATGGCAGAAG TTTCCGCAGC GGCTACAGTG AGAGGAGCCG GCACAGCAGC 
CACGATATGC TCAGCCACAG CTGGGAGGAC AGCAGGGAGA GGGGGCGTCC CCATCAGCGC 
GCACAGAGCC GGGAGCGGGA GCGCAGCCGT GGCCGGAGCC TGGAACGTGG CCTGGACCAT 
GAAGACTACG GGCGCAGCCA TGAGCGCAGC CGCGGCCGGA GCCTGGAGCG CGGTCTAGAC 
CGCGACTTTG TGTCCCGGGA CTACAGCCGT GGACGGAGCA TCGACAGGGA CTACGACCGA 
GACTATGAGC GCTCCTATCA CCAAGCCTAT GAGCCTGACT ACGAAGGCCG CTACAGCCCA 
TCTTATGACC GCAGAGCCCA TCCAGAGACC CGCTATGAAC GGAGCCGCAG CCGAGAACAC 
CTACGGTCCC GAAGCCCCAC CCCCGAGTCT AGGTCCCGGC AGGAACACAA GGGCCAGCAT 
GATCCCGACA GGCCCATCGG GGTCCTTCTG ACCAAAAGCA AAGCAAACGA AGAGTACGGG 
CTCCGGCTGG GAAGTCAGAT CTTCATTAAG GAAATGACCA GAACAGGACT GGCAACAAAA 
GATGGCAACC TTCATGAGGG GGACATAATT CTCAAGATCA ATGGAACTGT TACTGAGAAC 
ATGTCTTTAA CCGATGCTCG GAAGTTAATA GAAAAGTCTC GAGGAAAACT GCAGCTGGTG 
GTGTTGAGAG ACAGCAAGCA GACCCTCATC AACATCCCAT CTCTGAATGA CAGCGACTCG 
GAAGTGGAGG ATATCTCGGA AATCGAGTCC AACCGATCTT TTTCTCCAGA GGAGAGGCGC 
CAGCAGTATT CTGATCAGGA GTATCATTCC TCCACTGAGA AGCTGAAGGA GAGGCCAAGC 
TCAAGAGAGG AGACCTCAGG CAGAATGTCC AGGATGGGTG CCACACCCAC GCCGTTCAAG 
TCCACGGGGG ACATCACAGC TGCAGGTGTC ACAGAGGCCA ACAAGGAGCC CAGGTCCCAG 
GAGGAATCCC CAGTTCCTCA ACCCAGAACA GCATCAAGAG TCTTTCTTCG TCCTAGTCCC 
GAAGATGAAG CAATATATGG CCCTAACACC AAAATGGTGA AGTTCAAGAA GGGAGACAGC 
GTGGGCCTCC GGTTGGCTGG TGGAAATGAT GTTGGCATAT TTGTGGCTGG CATTCAGGAG 
GGCACCTCTG CAGAGCAGGA GGGCCTACAA GAAGGGGACC AGATTCTGAA GGTGAACACA 
CAAGATTTCA GAGGGCTGGT CCGGGAAGAT GCCGTCCTCT ACCTGTTAGA AATCCCTAAA 
GGTGAAACCG TGACCATTTT GGCTCAGAGC CGAGCAGACG TGTATAGAGA CATCCTGGCC 
TGTGGCAGGG GAGACTCGTT CTTCATAAGG AGCCACTTTG AATGTGAGAA GGAGACTCCG 
CAGAGCTTGG CCTTCACCAG GGGAGAGGTC TTCCGAGTGG TAGACACGCT GTACGATGGC 
AAACTGGGCC ACTGGCTGGC TGTGAGGATT GGAAATGAGC TGGAGAAGGG CTTAATCCCT 
AACAAAAGCA GAGCTGAGCA GATGGCCAGT GTCCAGAATG CCCAGCGAGA GAATGCTGGG 
GACAGAGCAG ACTTCTGGCG GATGCGTGGC CAGAGATCCG GGGTCAAGAA GAACATTCGC 
AAGAGCCGGG AAGACCTGGC AGCTGCTGTG TCGGTTAGCA CCAAGTTCCC CGCCTACGAG 
AAGGTTCTGC TTCGGGAAGC TGGCTTCAAG AGACCCGTGG TTCTGTTTGG CCCCATAGCA 
GATATAGCAA TGGAAAGGCT GACTACTGAG CTACCCGACC TGTTTCAAAC TGCAAAAACA 
GAACCCAAAG ATGCGGGATC TGAGAAATCC AGTGGAGTGG TTCGGTTGAA TACTGTGAGG 
CAAATTATTG AGCAGGACAA GCATGCCCTG CTCGACGTTA CCCCCAAAGC TGTGGACCTG 
CTCAATTATA CTCAGTGGTT CCCAATCGTG ATTTTCTTCA ACCCGGATTC CAGACAAGGC 
ATTAAAACCA TAAGGCAGAG GTTGAACCCA ACATCCAATA AAAGTTCTCG CAAGTTATTC 
GATCAAGCCA ACAAGCTCAA AAAAACCTGT TCTCATCTTT TTACAGCTAC CATCAACGTG 
AATTCAGCCA ATGATGGCTG GTTTGGCAGC CTGAAGGACA GCATTCAGCA GCAGCAAAAC 
GAAGCAGTGT GGGTCTCTGA AGGAAAGATG GAGGGGATGG ATGATGACGC TGAAGACCGC 
ATGTCCTACT TAACCGCCAT GGGTGCGGAC TATCTGAGTT GTGACAGCCG TCTCATCAGT 
GACTTTGAAG ATACGGACGG CGAGGGAGGC GCCTACACTG ACAATGAGCT GGATGAGCCA 
GCTGAGGAGC CGCTGGTGTC TTCCATCACC CGCTCCTCAG AGCCGGTGCA GCATGAGGAG 
AGCATAAGGA AGCCCAGCCC AGAGCCACGC GCTCAGATGA GGAGGGCAGC TAGCAGAGAC 
CAGCTTAGGG ATGGTAGCCC GCCCCCAGCA TTCAAGCCAG AGCCGCCCAA GGTCAGAAAC 
CAAAACAGAG AGGACTCTTT CAACTACTCC AAGTCAAACC TCTCTGCCAT GGCTGGCAGT 
GAAATCCCGG GGGGATCCAC CAAAGGGTGT CCTCCCCCTA TTGCGGTGAA ACCTGCCTTT 
GGGCGATCCA TCCTGAAGCC TTCTACTCCA GTCCCCATGC CTGAGAGTGA GGAGGTTGGG 
GAGAGCACCG AGGAGCAGGA AGAGGCTCCC AAATCAGTCC TGGGCAGAGT GAAAATCTTC 
GAGAAGATGG ACCACAAGGC GAAATTACAG AGGATGCAGG AGCTCCAAGA AGCACAGAAT 
GCGAGGATTG AAATAGCTCA GAAGCATCCT GACATCTATG CGGTTCCAAT CAAAGCCCCC 
AAGCCAGATG CTGGCCTGCC CCAGCACATG AGTTCTAGAC CCCCAGAGCC ACAGAAAGCT 
CCTTCTAGGC TTTACCAGGA CACCAGAGGA AGCTACGGCA GTGATCCCGA GGAAGAGGAG 
GAGTACCGCC AACAGTTGGC AGCACACTCG AAGCGTGGTT ACTACAGCCA GCCCTCCCGG 
TACCGAGACA CCGAATTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231243.1
CDS	411..3911
Translation	MEEVIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKIAAIVVKRPRKVQVAPLQGSPPLSHDDRGFEMMDEFDGRSFRSGYSERSRHSSHDMLSHSWEDSRERGRPHQRAQSRERERSRGRSLERGLDHEDYGRSHERSRGRSLERGLDRDFVSRDYSRGRSIDRDYDRDYERSYHQAYEPDYEGRYSPSYDRRAHPETRYERSRSREHLRSRSPTPESRSRQEHKGQHDPDRPIGVLLTKSKANEEYGLRLGSQIFIKEMTRTGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSKQTLINIPSLNDSDSEVEDISEIESNRSFSPEERRQQYSDQEYHSSTEKLKERPSSREETSGRMSRMGATPTPFKSTGDITAAGVTEANKEPRSQEESPVPQPRTASRVFLRPSPEDEAIYGPNTKMVKFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGLVREDAVLYLLEIPKGETVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEVFRVVDTLYDGKLGHWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRENAGDRADFWRMRGQRSGVKKNIRKSREDLAAAVSVSTKFPAYEKVLLREAGFKRPVVLFGPIADIAMERLTTELPDLFQTAKTEPKDAGSEKSSGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGIKTIRQRLNPTSNKSSRKLFDQANKLKKTCSHLFTATINVNSANDGWFGSLKDSIQQQQNEAVWVSEGKMEGMDDDAEDRMSYLTAMGADYLSCDSRLISDFEDTDGEGGAYTDNELDEPAEEPLVSSITRSSEPVQHEESIRKPSPEPRAQMRRAASRDQLRDGSPPPAFKPEPPKVRNQNREDSFNYSKSNLSAMAGSEIPGGSTKGCPPPIAVKPAFGRSILKPSTPVPMPESEEVGESTEEQEEAPKSVLGRVKIFEKMDHKAKLQRMQELQEAQNARIEIAQKHPDIYAVPIKAPKPDAGLPQHMSSRPPEPQKAPSRLYQDTRGSYGSDPEEEEEYRQQLAAHSKRGYYSQPSRYRDTEL

Target ORF information:

RefSeq Version	XM_006231181.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231181.3

ORF Insert Sequence:

ATGGAGGAGG TGATATGGGA ACAGTACACT GTGACCCTGC AGAAGGATTC CAAAAGAGGA 
TTTGGAATCG CAGTGTCAGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACGTCA 
ATCGTCATTT CAGATGTACT CCCAGGTGGA CCCGCTGATG GCTTGCTTCA AGAAAATGAC 
AGGGTGGTCA TGGTCAATGG CACCCCCATG GAGGATGTGC TCCATTCATT TGCCGTTCAG 
CAGCTTAGGA AAAGTGGGAA GATCGCAGCC ATTGTGGTCA AGAGGCCTCG GAAGGTTCAG 
GTGGCCCCGC TGCAGGGCAG CCCCCCTCTC AGTCATGATG ACCGAGGTTT TGAGATGATG 
GATGAATTTG ATGGCAGAAG TTTCCGCAGC GGCTACAGTG AGAGGAGCCG GCACAGCAGC 
CACGATATGC TCAGCCACAG CTGGGAGGAC AGCAGGGAGA GGGGGCGTCC CCATCAGCGC 
GCACAGAGCC GGGAGCGGGA GCGCAGCCGT GGCCGGAGCC TGGAACGTGG CCTGGACCAT 
GAAGACTACG GGCGCAGCCA TGAGCGCAGC CGCGGCCGGA GCCTGGAGCG CGGTCTAGAC 
CGCGACTTTG TGTCCCGGGA CTACAGCCGT GGACGGAGCA TCGACAGGGA CTACGACCGA 
GACTATGAGC GCTCCTATCA CCAAGCCTAT GAGCCTGACT ACGAAGGCCG CTACAGCCCA 
TCTTATGACC GCAGAGCCCA TCCAGAGACC CGCTATGAAC GGAGCCGCAG CCGAGAACAC 
CTACGGTCCC GAAGCCCCAC CCCCGAGTCT AGGTCCCGGC AGGAACACAA GGGCCAGCAT 
GATCCCGACA GGCCCATCGG GGTCCTTCTG ACCAAAAGCA AAGCAAACGA AGAGTACGGG 
CTCCGGCTGG GAAGTCAGAT CTTCATTAAG GAAATGACCA GAACAGGACT GGCAACAAAA 
GATGGCAACC TTCATGAGGG GGACATAATT CTCAAGATCA ATGGAACTGT TACTGAGAAC 
ATGTCTTTAA CCGATGCTCG GAAGTTAATA GAAAAGTCTC GAGGAAAACT GCAGCTGGTG 
GTGTTGAGAG ACAGCAAGCA GACCCTCATC AACATCCCAT CTCTGAATGA CAGCGACTCG 
GAAGTGGAGG ATATCTCGGA AATCGAGTCC AACCGATCTT TTTCTCCAGA GGAGAGGCGC 
CAGCAGTATT CTGATCAGGA GTATCATTCC TCCACTGAGA AGCTGAAGGA GAGGCCAAGC 
TCAAGAGAGG AGACCTCAGG CAGAATGTCC AGGATGGGTG CCACACCCAC GCCGTTCAAG 
TCCACGGGGG ACATCACAGC TGCAGGTGTC ACAGAGGCCA ACAAGGAGCC CAGGTCCCAG 
GAGGAATCCC CAGTTCCTCA ACCCAGAACA GCATCAAGAG TCTTTCTTCG TCCTAGTCCC 
GAAGATGAAG CAATATATGG CCCTAACACC AAAATGGTGA AGTTCAAGAA GGGAGACAGC 
GTGGGCCTCC GGTTGGCTGG TGGAAATGAT GTTGGCATAT TTGTGGCTGG CATTCAGGAG 
GGCACCTCTG CAGAGCAGGA GGGCCTACAA GAAGGGGACC AGATTCTGAA GGTGAACACA 
CAAGATTTCA GAGGGCTGGT CCGGGAAGAT GCCGTCCTCT ACCTGTTAGA AATCCCTAAA 
GGTGAAACCG TGACCATTTT GGCTCAGAGC CGAGCAGACG TGTATAGAGA CATCCTGGCC 
TGTGGCAGGG GAGACTCGTT CTTCATAAGG AGCCACTTTG AATGTGAGAA GGAGACTCCG 
CAGAGCTTGG CCTTCACCAG GGGAGAGGTC TTCCGAGTGG TAGACACGCT GTACGATGGC 
AAACTGGGCC ACTGGCTGGC TGTGAGGATT GGAAATGAGC TGGAGAAGGG CTTAATCCCT 
AACAAAAGCA GAGCTGAGCA GATGGCCAGT GTCCAGAATG CCCAGCGAGA GAATGCTGGG 
GACAGAGCAG ACTTCTGGCG GATGCGTGGC CAGAGATCCG GGGTCAAGAA GAACATTCGC 
AAGAGCCGGG AAGACCTGGC AGCTGCTGTG TCGGTTAGCA CCAAGTTCCC CGCCTACGAG 
AAGGTTCTGC TTCGGGAAGC TGGCTTCAAG AGACCCGTGG TTCTGTTTGG CCCCATAGCA 
GATATAGCAA TGGAAAGGCT GACTACTGAG CTACCCGACC TGTTTCAAAC TGCAAAAACA 
GAACCCAAAG ATGCGGGATC TGAGAAATCC AGTGGAGTGG TTCGGTTGAA TACTGTGAGG 
CAAATTATTG AGCAGGACAA GCATGCCCTG CTCGACGTTA CCCCCAAAGC TGTGGACCTG 
CTCAATTATA CTCAGTGGTT CCCAATCGTG ATTTTCTTCA ACCCGGATTC CAGACAAGGC 
ATTAAAACCA TAAGGCAGAG GTTGAACCCA ACATCCAATA AAAGTTCTCG CAAGTTATTC 
GATCAAGCCA ACAAGCTCAA AAAAACCTGT TCTCATCTTT TTACAGCTAC CATCAACGTG 
AATTCAGCCA ATGATGGCTG GTTTGGCAGC CTGAAGGACA GCATTCAGCA GCAGCAAAAC 
GAAGCAGTGT GGGTCTCTGA AGGAAAGATG GAGGGGATGG ATGATGACGC TGAAGACCGC 
ATGTCCTACT TAACCGCCAT GGGTGCGGAC TATCTGAGTT GTGACAGCCG TCTCATCAGT 
GACTTTGAAG ATACGGACGG CGAGGGAGGC GCCTACACTG ACAATGAGCT GGATGAGCCA 
GCTGAGGAGC CGCTGGTGTC TTCCATCACC CGCTCCTCAG AGCCGGTGCA GCATGAGGAG 
AGCATAAGGA AGCCCAGCCC AGAGCCACGC GCTCAGATGA GGAGGGCAGC TAGCAGAGAC 
CAGCTTAGGG ATGGTAGCCC GCCCCCAGCA TTCAAGCCAG AGCCGCCCAA GGTCAGAAAC 
CAAAACAGAG AGGACTCTTT CAACTACTCC AAGTCAAACC TCTCTGCCAT GGCTGGCAGT 
GAAATCCCGG GGGGATCCAC CAAAGGGTGT CCTCCCCCTA TTGCGGTGAA ACCTGCCTTT 
GGGCGATCCA TCCTGAAGCC TTCTACTCCA GTCCCCATGC CTGAGAGTGA GGAGGTTGGG 
GAGAGCACCG AGGAGCAGGA AGAGGCTCCC AAATCAGTCC TGGGCAGAGT GAAAATCTTC 
GAGAAGATGG ACCACAAGGC GAAATTACAG AGGATGCAGG AGCTCCAAGA AGCACAGAAT 
GCGAGGATTG AAATAGCTCA GAAGCATCCT GACATCTATG CGGTTCCAAT CAAAGCCCCC 
AAGCCAGATG CTGGCCTGCC CCAGCACATG AGTTCTAGAC CCCCAGAGCC ACAGAAAGCT 
CCTTCTAGGC TTTACCAGGA CACCAGAGGA AGCTACGGCA GTGATCCCGA GGAAGAGGAG 
GAGTACCGCC AACAGTTGGC AGCACACTCG AAGCGTGGTT ACTACAGCCA GCCCTCCCGG 
TACCGAGACA CCGAATTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp2 ( XM_008760302.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758524.1 Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X7, mRNA.

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CloneID	ORa23977
Clone ID Related Accession (Same CDS sequence)	XM_006231181.3 , XM_008760302.2
Accession Version	XM_008760302.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tight junction protein ZO-2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760302.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG TGATATGGGA ACAGTACACT GTGACCCTGC AGAAGGATTC CAAAAGAGGA 
TTTGGAATCG CAGTGTCAGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACGTCA 
ATCGTCATTT CAGATGTACT CCCAGGTGGA CCCGCTGATG GCTTGCTTCA AGAAAATGAC 
AGGGTGGTCA TGGTCAATGG CACCCCCATG GAGGATGTGC TCCATTCATT TGCCGTTCAG 
CAGCTTAGGA AAAGTGGGAA GATCGCAGCC ATTGTGGTCA AGAGGCCTCG GAAGGTTCAG 
GTGGCCCCGC TGCAGGGCAG CCCCCCTCTC AGTCATGATG ACCGAGGTTT TGAGATGATG 
GATGAATTTG ATGGCAGAAG TTTCCGCAGC GGCTACAGTG AGAGGAGCCG GCACAGCAGC 
CACGATATGC TCAGCCACAG CTGGGAGGAC AGCAGGGAGA GGGGGCGTCC CCATCAGCGC 
GCACAGAGCC GGGAGCGGGA GCGCAGCCGT GGCCGGAGCC TGGAACGTGG CCTGGACCAT 
GAAGACTACG GGCGCAGCCA TGAGCGCAGC CGCGGCCGGA GCCTGGAGCG CGGTCTAGAC 
CGCGACTTTG TGTCCCGGGA CTACAGCCGT GGACGGAGCA TCGACAGGGA CTACGACCGA 
GACTATGAGC GCTCCTATCA CCAAGCCTAT GAGCCTGACT ACGAAGGCCG CTACAGCCCA 
TCTTATGACC GCAGAGCCCA TCCAGAGACC CGCTATGAAC GGAGCCGCAG CCGAGAACAC 
CTACGGTCCC GAAGCCCCAC CCCCGAGTCT AGGTCCCGGC AGGAACACAA GGGCCAGCAT 
GATCCCGACA GGCCCATCGG GGTCCTTCTG ACCAAAAGCA AAGCAAACGA AGAGTACGGG 
CTCCGGCTGG GAAGTCAGAT CTTCATTAAG GAAATGACCA GAACAGGACT GGCAACAAAA 
GATGGCAACC TTCATGAGGG GGACATAATT CTCAAGATCA ATGGAACTGT TACTGAGAAC 
ATGTCTTTAA CCGATGCTCG GAAGTTAATA GAAAAGTCTC GAGGAAAACT GCAGCTGGTG 
GTGTTGAGAG ACAGCAAGCA GACCCTCATC AACATCCCAT CTCTGAATGA CAGCGACTCG 
GAAGTGGAGG ATATCTCGGA AATCGAGTCC AACCGATCTT TTTCTCCAGA GGAGAGGCGC 
CAGCAGTATT CTGATCAGGA GTATCATTCC TCCACTGAGA AGCTGAAGGA GAGGCCAAGC 
TCAAGAGAGG AGACCTCAGG CAGAATGTCC AGGATGGGTG CCACACCCAC GCCGTTCAAG 
TCCACGGGGG ACATCACAGC TGCAGGTGTC ACAGAGGCCA ACAAGGAGCC CAGGTCCCAG 
GAGGAATCCC CAGTTCCTCA ACCCAGAACA GCATCAAGAG TCTTTCTTCG TCCTAGTCCC 
GAAGATGAAG CAATATATGG CCCTAACACC AAAATGGTGA AGTTCAAGAA GGGAGACAGC 
GTGGGCCTCC GGTTGGCTGG TGGAAATGAT GTTGGCATAT TTGTGGCTGG CATTCAGGAG 
GGCACCTCTG CAGAGCAGGA GGGCCTACAA GAAGGGGACC AGATTCTGAA GGTGAACACA 
CAAGATTTCA GAGGGCTGGT CCGGGAAGAT GCCGTCCTCT ACCTGTTAGA AATCCCTAAA 
GGTGAAACCG TGACCATTTT GGCTCAGAGC CGAGCAGACG TGTATAGAGA CATCCTGGCC 
TGTGGCAGGG GAGACTCGTT CTTCATAAGG AGCCACTTTG AATGTGAGAA GGAGACTCCG 
CAGAGCTTGG CCTTCACCAG GGGAGAGGTC TTCCGAGTGG TAGACACGCT GTACGATGGC 
AAACTGGGCC ACTGGCTGGC TGTGAGGATT GGAAATGAGC TGGAGAAGGG CTTAATCCCT 
AACAAAAGCA GAGCTGAGCA GATGGCCAGT GTCCAGAATG CCCAGCGAGA GAATGCTGGG 
GACAGAGCAG ACTTCTGGCG GATGCGTGGC CAGAGATCCG GGGTCAAGAA GAACATTCGC 
AAGAGCCGGG AAGACCTGGC AGCTGCTGTG TCGGTTAGCA CCAAGTTCCC CGCCTACGAG 
AAGGTTCTGC TTCGGGAAGC TGGCTTCAAG AGACCCGTGG TTCTGTTTGG CCCCATAGCA 
GATATAGCAA TGGAAAGGCT GACTACTGAG CTACCCGACC TGTTTCAAAC TGCAAAAACA 
GAACCCAAAG ATGCGGGATC TGAGAAATCC AGTGGAGTGG TTCGGTTGAA TACTGTGAGG 
CAAATTATTG AGCAGGACAA GCATGCCCTG CTCGACGTTA CCCCCAAAGC TGTGGACCTG 
CTCAATTATA CTCAGTGGTT CCCAATCGTG ATTTTCTTCA ACCCGGATTC CAGACAAGGC 
ATTAAAACCA TAAGGCAGAG GTTGAACCCA ACATCCAATA AAAGTTCTCG CAAGTTATTC 
GATCAAGCCA ACAAGCTCAA AAAAACCTGT TCTCATCTTT TTACAGCTAC CATCAACGTG 
AATTCAGCCA ATGATGGCTG GTTTGGCAGC CTGAAGGACA GCATTCAGCA GCAGCAAAAC 
GAAGCAGTGT GGGTCTCTGA AGGAAAGATG GAGGGGATGG ATGATGACGC TGAAGACCGC 
ATGTCCTACT TAACCGCCAT GGGTGCGGAC TATCTGAGTT GTGACAGCCG TCTCATCAGT 
GACTTTGAAG ATACGGACGG CGAGGGAGGC GCCTACACTG ACAATGAGCT GGATGAGCCA 
GCTGAGGAGC CGCTGGTGTC TTCCATCACC CGCTCCTCAG AGCCGGTGCA GCATGAGGAG 
AGCATAAGGA AGCCCAGCCC AGAGCCACGC GCTCAGATGA GGAGGGCAGC TAGCAGAGAC 
CAGCTTAGGG ATGGTAGCCC GCCCCCAGCA TTCAAGCCAG AGCCGCCCAA GGTCAGAAAC 
CAAAACAGAG AGGACTCTTT CAACTACTCC AAGTCAAACC TCTCTGCCAT GGCTGGCAGT 
GAAATCCCGG GGGGATCCAC CAAAGGGTGT CCTCCCCCTA TTGCGGTGAA ACCTGCCTTT 
GGGCGATCCA TCCTGAAGCC TTCTACTCCA GTCCCCATGC CTGAGAGTGA GGAGGTTGGG 
GAGAGCACCG AGGAGCAGGA AGAGGCTCCC AAATCAGTCC TGGGCAGAGT GAAAATCTTC 
GAGAAGATGG ACCACAAGGC GAAATTACAG AGGATGCAGG AGCTCCAAGA AGCACAGAAT 
GCGAGGATTG AAATAGCTCA GAAGCATCCT GACATCTATG CGGTTCCAAT CAAAGCCCCC 
AAGCCAGATG CTGGCCTGCC CCAGCACATG AGTTCTAGAC CCCCAGAGCC ACAGAAAGCT 
CCTTCTAGGC TTTACCAGGA CACCAGAGGA AGCTACGGCA GTGATCCCGA GGAAGAGGAG 
GAGTACCGCC AACAGTTGGC AGCACACTCG AAGCGTGGTT ACTACAGCCA GCCCTCCCGG 
TACCGAGACA CCGAATTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758524.1
CDS	155..3655
Translation	MEEVIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKIAAIVVKRPRKVQVAPLQGSPPLSHDDRGFEMMDEFDGRSFRSGYSERSRHSSHDMLSHSWEDSRERGRPHQRAQSRERERSRGRSLERGLDHEDYGRSHERSRGRSLERGLDRDFVSRDYSRGRSIDRDYDRDYERSYHQAYEPDYEGRYSPSYDRRAHPETRYERSRSREHLRSRSPTPESRSRQEHKGQHDPDRPIGVLLTKSKANEEYGLRLGSQIFIKEMTRTGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSKQTLINIPSLNDSDSEVEDISEIESNRSFSPEERRQQYSDQEYHSSTEKLKERPSSREETSGRMSRMGATPTPFKSTGDITAAGVTEANKEPRSQEESPVPQPRTASRVFLRPSPEDEAIYGPNTKMVKFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGLVREDAVLYLLEIPKGETVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEVFRVVDTLYDGKLGHWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRENAGDRADFWRMRGQRSGVKKNIRKSREDLAAAVSVSTKFPAYEKVLLREAGFKRPVVLFGPIADIAMERLTTELPDLFQTAKTEPKDAGSEKSSGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGIKTIRQRLNPTSNKSSRKLFDQANKLKKTCSHLFTATINVNSANDGWFGSLKDSIQQQQNEAVWVSEGKMEGMDDDAEDRMSYLTAMGADYLSCDSRLISDFEDTDGEGGAYTDNELDEPAEEPLVSSITRSSEPVQHEESIRKPSPEPRAQMRRAASRDQLRDGSPPPAFKPEPPKVRNQNREDSFNYSKSNLSAMAGSEIPGGSTKGCPPPIAVKPAFGRSILKPSTPVPMPESEEVGESTEEQEEAPKSVLGRVKIFEKMDHKAKLQRMQELQEAQNARIEIAQKHPDIYAVPIKAPKPDAGLPQHMSSRPPEPQKAPSRLYQDTRGSYGSDPEEEEEYRQQLAAHSKRGYYSQPSRYRDTEL

Target ORF information:

RefSeq Version	XM_008760302.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760302.2

ORF Insert Sequence:

ATGGAGGAGG TGATATGGGA ACAGTACACT GTGACCCTGC AGAAGGATTC CAAAAGAGGA 
TTTGGAATCG CAGTGTCAGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACGTCA 
ATCGTCATTT CAGATGTACT CCCAGGTGGA CCCGCTGATG GCTTGCTTCA AGAAAATGAC 
AGGGTGGTCA TGGTCAATGG CACCCCCATG GAGGATGTGC TCCATTCATT TGCCGTTCAG 
CAGCTTAGGA AAAGTGGGAA GATCGCAGCC ATTGTGGTCA AGAGGCCTCG GAAGGTTCAG 
GTGGCCCCGC TGCAGGGCAG CCCCCCTCTC AGTCATGATG ACCGAGGTTT TGAGATGATG 
GATGAATTTG ATGGCAGAAG TTTCCGCAGC GGCTACAGTG AGAGGAGCCG GCACAGCAGC 
CACGATATGC TCAGCCACAG CTGGGAGGAC AGCAGGGAGA GGGGGCGTCC CCATCAGCGC 
GCACAGAGCC GGGAGCGGGA GCGCAGCCGT GGCCGGAGCC TGGAACGTGG CCTGGACCAT 
GAAGACTACG GGCGCAGCCA TGAGCGCAGC CGCGGCCGGA GCCTGGAGCG CGGTCTAGAC 
CGCGACTTTG TGTCCCGGGA CTACAGCCGT GGACGGAGCA TCGACAGGGA CTACGACCGA 
GACTATGAGC GCTCCTATCA CCAAGCCTAT GAGCCTGACT ACGAAGGCCG CTACAGCCCA 
TCTTATGACC GCAGAGCCCA TCCAGAGACC CGCTATGAAC GGAGCCGCAG CCGAGAACAC 
CTACGGTCCC GAAGCCCCAC CCCCGAGTCT AGGTCCCGGC AGGAACACAA GGGCCAGCAT 
GATCCCGACA GGCCCATCGG GGTCCTTCTG ACCAAAAGCA AAGCAAACGA AGAGTACGGG 
CTCCGGCTGG GAAGTCAGAT CTTCATTAAG GAAATGACCA GAACAGGACT GGCAACAAAA 
GATGGCAACC TTCATGAGGG GGACATAATT CTCAAGATCA ATGGAACTGT TACTGAGAAC 
ATGTCTTTAA CCGATGCTCG GAAGTTAATA GAAAAGTCTC GAGGAAAACT GCAGCTGGTG 
GTGTTGAGAG ACAGCAAGCA GACCCTCATC AACATCCCAT CTCTGAATGA CAGCGACTCG 
GAAGTGGAGG ATATCTCGGA AATCGAGTCC AACCGATCTT TTTCTCCAGA GGAGAGGCGC 
CAGCAGTATT CTGATCAGGA GTATCATTCC TCCACTGAGA AGCTGAAGGA GAGGCCAAGC 
TCAAGAGAGG AGACCTCAGG CAGAATGTCC AGGATGGGTG CCACACCCAC GCCGTTCAAG 
TCCACGGGGG ACATCACAGC TGCAGGTGTC ACAGAGGCCA ACAAGGAGCC CAGGTCCCAG 
GAGGAATCCC CAGTTCCTCA ACCCAGAACA GCATCAAGAG TCTTTCTTCG TCCTAGTCCC 
GAAGATGAAG CAATATATGG CCCTAACACC AAAATGGTGA AGTTCAAGAA GGGAGACAGC 
GTGGGCCTCC GGTTGGCTGG TGGAAATGAT GTTGGCATAT TTGTGGCTGG CATTCAGGAG 
GGCACCTCTG CAGAGCAGGA GGGCCTACAA GAAGGGGACC AGATTCTGAA GGTGAACACA 
CAAGATTTCA GAGGGCTGGT CCGGGAAGAT GCCGTCCTCT ACCTGTTAGA AATCCCTAAA 
GGTGAAACCG TGACCATTTT GGCTCAGAGC CGAGCAGACG TGTATAGAGA CATCCTGGCC 
TGTGGCAGGG GAGACTCGTT CTTCATAAGG AGCCACTTTG AATGTGAGAA GGAGACTCCG 
CAGAGCTTGG CCTTCACCAG GGGAGAGGTC TTCCGAGTGG TAGACACGCT GTACGATGGC 
AAACTGGGCC ACTGGCTGGC TGTGAGGATT GGAAATGAGC TGGAGAAGGG CTTAATCCCT 
AACAAAAGCA GAGCTGAGCA GATGGCCAGT GTCCAGAATG CCCAGCGAGA GAATGCTGGG 
GACAGAGCAG ACTTCTGGCG GATGCGTGGC CAGAGATCCG GGGTCAAGAA GAACATTCGC 
AAGAGCCGGG AAGACCTGGC AGCTGCTGTG TCGGTTAGCA CCAAGTTCCC CGCCTACGAG 
AAGGTTCTGC TTCGGGAAGC TGGCTTCAAG AGACCCGTGG TTCTGTTTGG CCCCATAGCA 
GATATAGCAA TGGAAAGGCT GACTACTGAG CTACCCGACC TGTTTCAAAC TGCAAAAACA 
GAACCCAAAG ATGCGGGATC TGAGAAATCC AGTGGAGTGG TTCGGTTGAA TACTGTGAGG 
CAAATTATTG AGCAGGACAA GCATGCCCTG CTCGACGTTA CCCCCAAAGC TGTGGACCTG 
CTCAATTATA CTCAGTGGTT CCCAATCGTG ATTTTCTTCA ACCCGGATTC CAGACAAGGC 
ATTAAAACCA TAAGGCAGAG GTTGAACCCA ACATCCAATA AAAGTTCTCG CAAGTTATTC 
GATCAAGCCA ACAAGCTCAA AAAAACCTGT TCTCATCTTT TTACAGCTAC CATCAACGTG 
AATTCAGCCA ATGATGGCTG GTTTGGCAGC CTGAAGGACA GCATTCAGCA GCAGCAAAAC 
GAAGCAGTGT GGGTCTCTGA AGGAAAGATG GAGGGGATGG ATGATGACGC TGAAGACCGC 
ATGTCCTACT TAACCGCCAT GGGTGCGGAC TATCTGAGTT GTGACAGCCG TCTCATCAGT 
GACTTTGAAG ATACGGACGG CGAGGGAGGC GCCTACACTG ACAATGAGCT GGATGAGCCA 
GCTGAGGAGC CGCTGGTGTC TTCCATCACC CGCTCCTCAG AGCCGGTGCA GCATGAGGAG 
AGCATAAGGA AGCCCAGCCC AGAGCCACGC GCTCAGATGA GGAGGGCAGC TAGCAGAGAC 
CAGCTTAGGG ATGGTAGCCC GCCCCCAGCA TTCAAGCCAG AGCCGCCCAA GGTCAGAAAC 
CAAAACAGAG AGGACTCTTT CAACTACTCC AAGTCAAACC TCTCTGCCAT GGCTGGCAGT 
GAAATCCCGG GGGGATCCAC CAAAGGGTGT CCTCCCCCTA TTGCGGTGAA ACCTGCCTTT 
GGGCGATCCA TCCTGAAGCC TTCTACTCCA GTCCCCATGC CTGAGAGTGA GGAGGTTGGG 
GAGAGCACCG AGGAGCAGGA AGAGGCTCCC AAATCAGTCC TGGGCAGAGT GAAAATCTTC 
GAGAAGATGG ACCACAAGGC GAAATTACAG AGGATGCAGG AGCTCCAAGA AGCACAGAAT 
GCGAGGATTG AAATAGCTCA GAAGCATCCT GACATCTATG CGGTTCCAAT CAAAGCCCCC 
AAGCCAGATG CTGGCCTGCC CCAGCACATG AGTTCTAGAC CCCCAGAGCC ACAGAAAGCT 
CCTTCTAGGC TTTACCAGGA CACCAGAGGA AGCTACGGCA GTGATCCCGA GGAAGAGGAG 
GAGTACCGCC AACAGTTGGC AGCACACTCG AAGCGTGGTT ACTACAGCCA GCCCTCCCGG 
TACCGAGACA CCGAATTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp2 ( XM_006231182.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231244.1 Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa23978
Clone ID Related Accession (Same CDS sequence)	XM_006231182.3
Accession Version	XM_006231182.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tight junction protein ZO-2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231182.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGCTG CTCAGGTCTT CCAGAGGATG TGGATCCAGG CAGTTAAAAA GTTGGAGAGA 
TGGAAAGGCC ATGGCCCAGG CATGGAGGAG GTGATATGGG AACAGTACAC TGTGACCCTG 
CAGAAGGATT CCAAAAGAGG ATTTGGAATC GCAGTGTCAG GAGGCAGAGA CAACCCCCAC 
TTTGAAAATG GGGAGACGTC AATCGTCATT TCAGATGTAC TCCCAGGTGG ACCCGCTGAT 
GGCTTGCTTC AAGAAAATGA CAGGGTGGTC ATGGTCAATG GCACCCCCAT GGAGGATGTG 
CTCCATTCAT TTGCCGTTCA GCAGCTTAGG AAAAGTGGGA AGATCGCAGC CATTGTGGTC 
AAGAGGCCTC GGAAGGTTCA GGTGGCCCCG CTGCAGGGCA GCCCCCCTCT CAGTCATGAT 
GACCGAGGTT TTGAGATGAT GGATGAATTT GATGGCAGAA GTTTCCGCAG CGGCTACAGT 
GAGAGGAGCC GGCACAGCAG CCACGATATG CTCAGCCACA GCTGGGAGGA CAGCAGGGAG 
AGGGGGCGTC CCCATCAGCG CGCACAGAGC CGGGAGCGGG AGCGCAGCCG TGGCCGGAGC 
CTGGAACGTG GCCTGGACCA TGAAGACTAC GGGCGCAGCC ATGAGCGCAG CCGCGGCCGG 
AGCCTGGAGC GCGGTCTAGA CCGCGACTTT GTGTCCCGGG ACTACAGCCG TGGACGGAGC 
ATCGACAGGG ACTACGACCG AGACTATGAG CGCTCCTATC ACCAAGCCTA TGAGCCTGAC 
TACGAAGGCC GCTACAGCCC ATCTTATGAC CGCAGAGCCC ATCCAGAGAC CCGCTATGAA 
CGGAGCCGCA GCCGAGAACA CCTACGGTCC CGAAGCCCCA CCCCCGAGTC TAGGTCCCGG 
CAGGAACACA AGGGCCAGCA TGATCCCGAC AGGCCCATCG GGGTCCTTCT GACCAAAAGC 
AAAGCAAACG AAGAGTACGG GCTCCGGCTG GGAAGTCAGA TCTTCATTAA GGAAATGACC 
AGAACAGGAC TGGCAACAAA AGATGGCAAC CTTCATGAGG GGGACATAAT TCTCAAGATC 
AATGGAACTG TTACTGAGAA CATGTCTTTA ACCGATGCTC GGAAGTTAAT AGAAAAGTCT 
CGAGGAAAAC TGCAGCTGGT GGTGTTGAGA GACAGCAAGC AGACCCTCAT CAACATCCCA 
TCTCTGAATG ACAGCGACTC GGAAGTGGAG GATATCTCGG AAATCGAGTC CAACCGATCT 
TTTTCTCCAG AGGAGAGGCG CCAGCAGTAT TCTGATCAGG AGTATCATTC CTCCACTGAG 
AAGCTGAAGG AGAGGCCAAG CTCAAGAGAG GAGACCTCAG GCAGAATGTC CAGGATGGGT 
GCCACACCCA CGCCGTTCAA GTCCACGGGG GACATCACAG CTGCAGGTGT CACAGAGGCC 
AACAAGGAGC CCAGGTCCCA GGAGGAATCC CCAGTTCCTC AACCCAGAAC AGCATCAAGA 
GTCTTTCTTC GTCCTAGTCC CGAAGATGAA GCAATATATG GCCCTAACAC CAAAATGGTG 
AAGTTCAAGA AGGGAGACAG CGTGGGCCTC CGGTTGGCTG GTGGAAATGA TGTTGGCATA 
TTTGTGGCTG GCATTCAGGA GGGCACCTCT GCAGAGCAGG AGGGCCTACA AGAAGGGGAC 
CAGATTCTGA AGGTGAACAC ACAAGATTTC AGAGGGCTGG TCCGGGAAGA TGCCGTCCTC 
TACCTGTTAG AAATCCCTAA AGGTGAAACC GTGACCATTT TGGCTCAGAG CCGAGCAGAC 
GTGTATAGAG ACATCCTGGC CTGTGGCAGG GGAGACTCGT TCTTCATAAG GAGCCACTTT 
GAATGTGAGA AGGAGACTCC GCAGAGCTTG GCCTTCACCA GGGGAGAGGT CTTCCGAGTG 
GTAGACACGC TGTACGATGG CAAACTGGGC CACTGGCTGG CTGTGAGGAT TGGAAATGAG 
CTGGAGAAGG GCTTAATCCC TAACAAAAGC AGAGCTGAGC AGATGGCCAG TGTCCAGAAT 
GCCCAGCGAG AGAATGCTGG GGACAGAGCA GACTTCTGGC GGATGCGTGG CCAGAGATCC 
GGGGTCAAGA AGAACATTCG CAAGAGCCGG GAAGACCTGG CAGCTGCTGT GTCGGTTAGC 
ACCAAGTTCC CCGCCTACGA GAAGGTTCTG CTTCGGGAAG CTGGCTTCAA GAGACCCGTG 
GTTCTGTTTG GCCCCATAGC AGATATAGCA ATGGAAAGGC TGACTACTGA GCTACCCGAC 
CTGTTTCAAA CTGCAAAAAC AGAACCCAAA GATGCGGGAT CTGAGAAATC CAGTGGAGTG 
GTTCGGTTGA ATACTGTGAG GCAAATTATT GAGCAGGACA AGCATGCCCT GCTCGACGTT 
ACCCCCAAAG CTGTGGACCT GCTCAATTAT ACTCAGTGGT TCCCAATCGT GATTTTCTTC 
AACCCGGATT CCAGACAAGG CATTAAAACC ATAAGGCAGA GGTTGAACCC AACATCCAAT 
AAAAGTTCTC GCAAGTTATT CGATCAAGCC AACAAGCTCA AAAAAACCTG TTCTCATCTT 
TTTACAGCTA CCATCAACGT GAATTCAGCC AATGATGGCT GGTTTGGCAG CCTGAAGGAC 
AGCATTCAGC AGCAGCAAAA CGAAGCAGTG TGGGTCTCTG AAGGAAAGAT GGAGGGGATG 
GATGATGACG CTGAAGACCG CATGTCCTAC TTAACCGCCA TGGGTGCGGA CTATCTGAGT 
TGTGACAGCC GTCTCATCAG TGACTTTGAA GATACGGACG GCGAGGGAGG CGCCTACACT 
GACAATGAGC TGGATGAGCC AGCTGAGGAG CCGCTGGTGT CTTCCATCAC CCGCTCCTCA 
GAGCCGGTGC AGCATGAGGA GGTCAGAAAC CAAAACAGAG AGGACTCTTT CAACTACTCC 
AAGTCAAACC TCTCTGCCAT GGCTGGCAGT GAAATCCCGG GGGGATCCAC CAAAGGGTGT 
CCTCCCCCTA TTGCGGTGAA ACCTGCCTTT GGGCGATCCA TCCTGAAGCC TTCTACTCCA 
GTCCCCATGC CTGAGAGTGA GGAGGTTGGG GAGAGCACCG AGGAGCAGGA AGAGGCTCCC 
AAATCAGTCC TGGGCAGAGT GAAAATCTTC GAGAAGATGG ACCACAAGGC GAAATTACAG 
AGGATGCAGG AGCTCCAAGA AGCACAGAAT GCGAGGATTG AAATAGCTCA GAAGCATCCT 
GACATCTATG CGGTTCCAAT CAAAGCCCCC AAGCCAGATG CTGGCCTGCC CCAGCACATG 
AGTTCTAGAC CCCCAGAGCC ACAGAAAGCT CCTTCTAGGC TTTACCAGGA CACCAGAGGA 
AGCTACGGCA GTGATCCCGA GGAAGAGGAG GAGTACCGCC AACAGTTGGC AGCACACTCG 
AAGCGTGGTT ACTACAGCCA GCCCTCCCGG TACCGAGACA CCGAATTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231244.1
CDS	540..4010
Translation	MRAAQVFQRMWIQAVKKLERWKGHGPGMEEVIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKIAAIVVKRPRKVQVAPLQGSPPLSHDDRGFEMMDEFDGRSFRSGYSERSRHSSHDMLSHSWEDSRERGRPHQRAQSRERERSRGRSLERGLDHEDYGRSHERSRGRSLERGLDRDFVSRDYSRGRSIDRDYDRDYERSYHQAYEPDYEGRYSPSYDRRAHPETRYERSRSREHLRSRSPTPESRSRQEHKGQHDPDRPIGVLLTKSKANEEYGLRLGSQIFIKEMTRTGLATKDGNLHEGDIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSKQTLINIPSLNDSDSEVEDISEIESNRSFSPEERRQQYSDQEYHSSTEKLKERPSSREETSGRMSRMGATPTPFKSTGDITAAGVTEANKEPRSQEESPVPQPRTASRVFLRPSPEDEAIYGPNTKMVKFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILKVNTQDFRGLVREDAVLYLLEIPKGETVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTRGEVFRVVDTLYDGKLGHWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRENAGDRADFWRMRGQRSGVKKNIRKSREDLAAAVSVSTKFPAYEKVLLREAGFKRPVVLFGPIADIAMERLTTELPDLFQTAKTEPKDAGSEKSSGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGIKTIRQRLNPTSNKSSRKLFDQANKLKKTCSHLFTATINVNSANDGWFGSLKDSIQQQQNEAVWVSEGKMEGMDDDAEDRMSYLTAMGADYLSCDSRLISDFEDTDGEGGAYTDNELDEPAEEPLVSSITRSSEPVQHEEVRNQNREDSFNYSKSNLSAMAGSEIPGGSTKGCPPPIAVKPAFGRSILKPSTPVPMPESEEVGESTEEQEEAPKSVLGRVKIFEKMDHKAKLQRMQELQEAQNARIEIAQKHPDIYAVPIKAPKPDAGLPQHMSSRPPEPQKAPSRLYQDTRGSYGSDPEEEEEYRQQLAAHSKRGYYSQPSRYRDTEL

Target ORF information:

RefSeq Version	XM_006231182.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231182.3

ORF Insert Sequence:

ATGAGGGCTG CTCAGGTCTT CCAGAGGATG TGGATCCAGG CAGTTAAAAA GTTGGAGAGA 
TGGAAAGGCC ATGGCCCAGG CATGGAGGAG GTGATATGGG AACAGTACAC TGTGACCCTG 
CAGAAGGATT CCAAAAGAGG ATTTGGAATC GCAGTGTCAG GAGGCAGAGA CAACCCCCAC 
TTTGAAAATG GGGAGACGTC AATCGTCATT TCAGATGTAC TCCCAGGTGG ACCCGCTGAT 
GGCTTGCTTC AAGAAAATGA CAGGGTGGTC ATGGTCAATG GCACCCCCAT GGAGGATGTG 
CTCCATTCAT TTGCCGTTCA GCAGCTTAGG AAAAGTGGGA AGATCGCAGC CATTGTGGTC 
AAGAGGCCTC GGAAGGTTCA GGTGGCCCCG CTGCAGGGCA GCCCCCCTCT CAGTCATGAT 
GACCGAGGTT TTGAGATGAT GGATGAATTT GATGGCAGAA GTTTCCGCAG CGGCTACAGT 
GAGAGGAGCC GGCACAGCAG CCACGATATG CTCAGCCACA GCTGGGAGGA CAGCAGGGAG 
AGGGGGCGTC CCCATCAGCG CGCACAGAGC CGGGAGCGGG AGCGCAGCCG TGGCCGGAGC 
CTGGAACGTG GCCTGGACCA TGAAGACTAC GGGCGCAGCC ATGAGCGCAG CCGCGGCCGG 
AGCCTGGAGC GCGGTCTAGA CCGCGACTTT GTGTCCCGGG ACTACAGCCG TGGACGGAGC 
ATCGACAGGG ACTACGACCG AGACTATGAG CGCTCCTATC ACCAAGCCTA TGAGCCTGAC 
TACGAAGGCC GCTACAGCCC ATCTTATGAC CGCAGAGCCC ATCCAGAGAC CCGCTATGAA 
CGGAGCCGCA GCCGAGAACA CCTACGGTCC CGAAGCCCCA CCCCCGAGTC TAGGTCCCGG 
CAGGAACACA AGGGCCAGCA TGATCCCGAC AGGCCCATCG GGGTCCTTCT GACCAAAAGC 
AAAGCAAACG AAGAGTACGG GCTCCGGCTG GGAAGTCAGA TCTTCATTAA GGAAATGACC 
AGAACAGGAC TGGCAACAAA AGATGGCAAC CTTCATGAGG GGGACATAAT TCTCAAGATC 
AATGGAACTG TTACTGAGAA CATGTCTTTA ACCGATGCTC GGAAGTTAAT AGAAAAGTCT 
CGAGGAAAAC TGCAGCTGGT GGTGTTGAGA GACAGCAAGC AGACCCTCAT CAACATCCCA 
TCTCTGAATG ACAGCGACTC GGAAGTGGAG GATATCTCGG AAATCGAGTC CAACCGATCT 
TTTTCTCCAG AGGAGAGGCG CCAGCAGTAT TCTGATCAGG AGTATCATTC CTCCACTGAG 
AAGCTGAAGG AGAGGCCAAG CTCAAGAGAG GAGACCTCAG GCAGAATGTC CAGGATGGGT 
GCCACACCCA CGCCGTTCAA GTCCACGGGG GACATCACAG CTGCAGGTGT CACAGAGGCC 
AACAAGGAGC CCAGGTCCCA GGAGGAATCC CCAGTTCCTC AACCCAGAAC AGCATCAAGA 
GTCTTTCTTC GTCCTAGTCC CGAAGATGAA GCAATATATG GCCCTAACAC CAAAATGGTG 
AAGTTCAAGA AGGGAGACAG CGTGGGCCTC CGGTTGGCTG GTGGAAATGA TGTTGGCATA 
TTTGTGGCTG GCATTCAGGA GGGCACCTCT GCAGAGCAGG AGGGCCTACA AGAAGGGGAC 
CAGATTCTGA AGGTGAACAC ACAAGATTTC AGAGGGCTGG TCCGGGAAGA TGCCGTCCTC 
TACCTGTTAG AAATCCCTAA AGGTGAAACC GTGACCATTT TGGCTCAGAG CCGAGCAGAC 
GTGTATAGAG ACATCCTGGC CTGTGGCAGG GGAGACTCGT TCTTCATAAG GAGCCACTTT 
GAATGTGAGA AGGAGACTCC GCAGAGCTTG GCCTTCACCA GGGGAGAGGT CTTCCGAGTG 
GTAGACACGC TGTACGATGG CAAACTGGGC CACTGGCTGG CTGTGAGGAT TGGAAATGAG 
CTGGAGAAGG GCTTAATCCC TAACAAAAGC AGAGCTGAGC AGATGGCCAG TGTCCAGAAT 
GCCCAGCGAG AGAATGCTGG GGACAGAGCA GACTTCTGGC GGATGCGTGG CCAGAGATCC 
GGGGTCAAGA AGAACATTCG CAAGAGCCGG GAAGACCTGG CAGCTGCTGT GTCGGTTAGC 
ACCAAGTTCC CCGCCTACGA GAAGGTTCTG CTTCGGGAAG CTGGCTTCAA GAGACCCGTG 
GTTCTGTTTG GCCCCATAGC AGATATAGCA ATGGAAAGGC TGACTACTGA GCTACCCGAC 
CTGTTTCAAA CTGCAAAAAC AGAACCCAAA GATGCGGGAT CTGAGAAATC CAGTGGAGTG 
GTTCGGTTGA ATACTGTGAG GCAAATTATT GAGCAGGACA AGCATGCCCT GCTCGACGTT 
ACCCCCAAAG CTGTGGACCT GCTCAATTAT ACTCAGTGGT TCCCAATCGT GATTTTCTTC 
AACCCGGATT CCAGACAAGG CATTAAAACC ATAAGGCAGA GGTTGAACCC AACATCCAAT 
AAAAGTTCTC GCAAGTTATT CGATCAAGCC AACAAGCTCA AAAAAACCTG TTCTCATCTT 
TTTACAGCTA CCATCAACGT GAATTCAGCC AATGATGGCT GGTTTGGCAG CCTGAAGGAC 
AGCATTCAGC AGCAGCAAAA CGAAGCAGTG TGGGTCTCTG AAGGAAAGAT GGAGGGGATG 
GATGATGACG CTGAAGACCG CATGTCCTAC TTAACCGCCA TGGGTGCGGA CTATCTGAGT 
TGTGACAGCC GTCTCATCAG TGACTTTGAA GATACGGACG GCGAGGGAGG CGCCTACACT 
GACAATGAGC TGGATGAGCC AGCTGAGGAG CCGCTGGTGT CTTCCATCAC CCGCTCCTCA 
GAGCCGGTGC AGCATGAGGA GGTCAGAAAC CAAAACAGAG AGGACTCTTT CAACTACTCC 
AAGTCAAACC TCTCTGCCAT GGCTGGCAGT GAAATCCCGG GGGGATCCAC CAAAGGGTGT 
CCTCCCCCTA TTGCGGTGAA ACCTGCCTTT GGGCGATCCA TCCTGAAGCC TTCTACTCCA 
GTCCCCATGC CTGAGAGTGA GGAGGTTGGG GAGAGCACCG AGGAGCAGGA AGAGGCTCCC 
AAATCAGTCC TGGGCAGAGT GAAAATCTTC GAGAAGATGG ACCACAAGGC GAAATTACAG 
AGGATGCAGG AGCTCCAAGA AGCACAGAAT GCGAGGATTG AAATAGCTCA GAAGCATCCT 
GACATCTATG CGGTTCCAAT CAAAGCCCCC AAGCCAGATG CTGGCCTGCC CCAGCACATG 
AGTTCTAGAC CCCCAGAGCC ACAGAAAGCT CCTTCTAGGC TTTACCAGGA CACCAGAGGA 
AGCTACGGCA GTGATCCCGA GGAAGAGGAG GAGTACCGCC AACAGTTGGC AGCACACTCG 
AAGCGTGGTT ACTACAGCCA GCCCTCCCGG TACCGAGACA CCGAATTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ZO-1 and -2 Are Required for TRPV1-Modulated Paracellular Permeability.
Journal of dental research94(12)1748-56(2015 Dec)
Li J,Cong X,Zhang Y,Xiang RL,Mei M,Yang NY,Su YC,Choi S,Park K,Zhang LW,Wu LL,Yu GY

Progressive Hearing Loss in Mice Carrying a Mutation in Usp53.
The Journal of neuroscience : the official journal of the Society for Neuroscience35(47)15582-98(2015 Nov)
Kazmierczak M,Harris SL,Kazmierczak P,Shah P,Starovoytov V,Ohlemiller KK,Schwander M

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Science signaling7(354)rs7(2014 Dec)
Guo Z,Neilson LJ,Zhong H,Murray PS,Zanivan S,Zaidel-Bar R

JNK1/2-dependent phosphorylation of angulin-1/LSR is required for the exclusive localization of angulin-1/LSR and tricellulin at tricellular contacts in EpH4 epithelial sheet.
Genes to cells : devoted to molecular & cellular mechanisms19(7)565-81(2014 Jul)
Nakatsu D,Kano F,Taguchi Y,Sugawara T,Nishizono T,Nishikawa K,Oda Y,Furuse M,Murata M

Different effects of ZO-1, ZO-2 and ZO-3 silencing on kidney collecting duct principal cell proliferation and adhesion.
Cell cycle (Georgetown, Tex.)13(19)3059-75(2014)
Qiao X,Roth I,F?raille E,Hasler U

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Tjp2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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