Tjp2 cDNA ORF clone, Rattus norvegicus(Norway rat)

The following Tjp2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tjp2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa10600 NM_053773.1
Latest version!
Rattus norvegicus tight junction protein 2 (Tjp2), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
ORa23971 XM_006231175.3
Latest version!
Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
ORa23972 XM_006231176.3
Latest version!
Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
ORa23973 XM_006231177.3
Latest version!
Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
ORa23974 XM_006231178.3
Latest version!
Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
ORa23977 XM_008760302.2
Latest version!
Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
ORa23977 XM_006231181.3
Latest version!
Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
ORa23978 XM_006231182.3
Latest version!
Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ORa10600
    Clone ID Related Accession (Same CDS sequence) NM_053773.1
    Accession Version NM_053773.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-09
    Organism Rattus norvegicus(Norway rat)
    Product tight junction protein ZO-2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC103479.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC103479.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGGAGG TGATATGGGA ACAGTACACT GTGACCCTGC AGAAGGATTC CAAAAGAGGA 
    TTTGGAATCG CAGTGTCAGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACGTCA
    ATCGTCATTT CAGATGTACT CCCAGGTGGA CCCGCTGATG GCTTGCTTCA AGAAAATGAC
    AGGGTGGTCA TGGTCAATGG CACCCCCATG GAGGATGTGC TCCATTCATT TGCCGTTCAG
    CAGCTTAGGA AAAGTGGGAA GATCGCAGCC ATTGTGGTCA AGAGGCCTCG GAAGGTTCAG
    GTGGCCCCGC TGCAGGGCAG CCCCCCTCTC AGTCATGATG ACCGAGGTTT TGAGATGATG
    GATGAATTTG ATGGCAGAAG TTTCCGCAGC GGCTACAGTG AGAGGAGCCG GCACAGCAGC
    CACGATATGC TCAGCCACAG CTGGGAGGAC AGCAGGGAGA GGGGGCGTCC CCATCAGCGC
    GCACAGAGCC GGGAGCGGGA GCGCAGCCGT GGCCGGAGCC TGGAACGTGG CCTGGACCAT
    GAAGACTACG GGCGCAGCCA TGAGCGCAGC CGCGGCCGGA GCCTGGAGCG CGGTCTAGAC
    CGCGACTTTG TGTCCCGGGA CTACAGCCGT GGACGGAGCA TCGACAGGGA CTACGACCGA
    GACTATGAGC GCTCCTATCA CCAAGCCTAT GAGCCTGACT ACGAAGGCCG CTACAGCCCA
    TCTTATGACC GCAGAGCCCA TCCAGAGACC CGCTATGAAC GGAGCCGCAG CCGAGAACAC
    CTACGGTCCC GAAGCCCCAC CCCCGAGTCT AGGTCCCGGC AGGAACACAA GGGCCAGCAT
    GATCCCGACA GGCCCATCGG GGTCCTTCTG ACCAAAAGCA AAGCAAACGA AGAGTACGGG
    CTCCGGCTGG GAAGTCAGAT CTTCATTAAG GAAATGACCA GAACAGGACT GGCAACAAAA
    GATGGCAACC TTCATGAGGG GGACATAATT CTCAAGATCA ATGGAACTGT TACTGAGAAC
    ATGTCTTTAA CCGATGCTCG GAAGTTAATA GAAAAGTCTC GAGGAAAACT GCAGCTGGTG
    GTGTTGAGAG ACAGCAAGCA GACCCTCATC AACATCCCAT CTCTGAATGA CAGCGACTCG
    GAAGTGGAGG ATATCTCGGA AATCGAGTCC AACCGATCTT TTTCTCCAGA GGAGAGGCGC
    CAGCAGTATT CTGATCAGGA GTATCATTCC TCCACTGAGA AGCTGAAGGA GAGGCCAAGC
    TCAAGAGAGG AGACCTCAGG CAGAATGTCC AGGATGGGTG CCACACCCAC GCCGTTCAAG
    TCCACGGGGG ACATCACAGC TGCAGGTGTC ACAGAGGCCA ACAAGGAGCC CAGGTCCCAG
    GAGGAATCCC CAGTTCCTCA ACCCAGAACA GCATCAAGAG TCTTTCTTCG TCCTAGTCCC
    GAAGATGAAG CAATATATGG CCCTAACACC AAAATGGTGA AGTTCAAGAA GGGAGACAGC
    GTGGGCCTCC GGTTGGCTGG TGGAAATGAT GTTGGCATAT TTGTGGCTGG CATTCAGGAG
    GGCACCTCTG CAGAGCAGGA GGGCCTACAA GAAGGGGACC AGATTCTGAA GGTGAACACA
    CAAGATTTCA GAGGGCTGGT CCGGGAAGAT GCCGTCCTCT ACCTGTTAGA AATCCCTAAA
    GGTGAAACCG TGACCATTTT GGCTCAGAGC CGAGCAGACG TGTATAGAGA CATCCTGGCC
    TGTGGCAGGG GAGACTCGTT CTTCATAAGG AGCCACTTTG AATGTGAGAA GGAGACTCCG
    CAGAGCTTGG CCTTCACCAG GGGAGAGGTC TTCCGAGTGG TAGACACGCT GTACGATGGC
    AAACTGGGCC ACTGGCTGGC TGTGAGGATT GGAAATGAGC TGGAGAAGGG CTTAATCCCT
    AACAAAAGCA GAGCTGAGCA GATGGCCAGT GTCCAGAATG CCCAGCGAGA GAATGCTGGG
    GACAGAGCAG ACTTCTGGCG GATGCGTGGC CAGAGATCCG GGGTCAAGAA GAACATTCGC
    AAGAGCCGGG AAGACCTGGC AGCTGCTGTG TCGGTTAGCA CCAAGTTCCC CGCCTACGAG
    AAGGTTCTGC TTCGGGAAGC TGGCTTCAAG AGACCCGTGG TTCTGTTTGG CCCCATAGCA
    GATATAGCAA TGGAAAGGCT GACTACTGAG CTACCCGACC TGTTTCAAAC TGCAAAAACA
    GAACCCAAAG ATGCGGGATC TGAGAAATCC AGTGGAGTGG TTCGGTTGAA TACTGTGAGG
    CAAATTATTG AGCAGGACAA GCATGCCCTG CTCGACGTTA CCCCCAAAGC TGTGGACCTG
    CTCAATTATA CTCAGTGGTT CCCAATCGTG ATTTTCTTCA ACCCGGATTC CAGACAAGGC
    ATTAAAACCA TAAGGCAGAG GTTGAACCCA ACATCCAATA AAAGTTCTCG CAAGTTATTC
    GATCAAGCCA ACAAGCTCAA AAAAACCTGT TCTCATCTTT TTACAGCTAC CATCAACGTG
    AATTCAGCCA ATGATGGCTG GTTTGGCAGC CTGAAGGACA GCATTCAGCA GCAGCAAAAC
    GAAGCAGTGT GGGTCTCTGA AGGAAAGATG GAGGGGATGG ATGATGACGC TGAAGACCGC
    ATGTCCTACT TAACCGCCAT GGGTGCGGAC TATCTGAGTT GTGACAGCCG TCTCATCAGT
    GACTTTGAAG ATACGGACGG CGAGGGAGGC GCCTACACTG ACAATGAGCT GGATGAGCCA
    GCTGAGGAGC CGCTGGTGTC TTCCATCACC CGCTCCTCAG AGCCGGTGCA GCATGAGGAG
    AGCATAAGGA AGCCCAGCCC AGAGCCACGC GCTCAGATGA GGAGGGCAGC TAGCAGAGAC
    CAGCTTAGGG ATGGTAGCCC GCCCCCAGCA TTCAAGCCAG AGCCGCCCAA GGTCAGAAAC
    CAAAACAGAG AGGACTCTTT CAACTACTCC AAGTCAAACC TCTCTGCCAT GGCTGGCAGT
    GAAATCCCGG GGGGATCCAC CAAAGGGTGT CCTCCCCCTA TTGCGGTGAA ACCTGCCTTT
    GGGCGATCCA TCCTGAAGCC TTCTACTCCA GTCCCCATGC CTGAGAGTGA GGAGGTTGGG
    GAGAGCACCG AGGAGCAGGA AGAGGCTCCC AAATCAGTCC TGGGCAGAGT GAAAATCTTC
    GAGAAGATGG ACCACAAGGC GAAATTACAG AGGATGCAGG AGCTCCAAGA AGCACAGAAT
    GCGAGGATTG AAATAGCTCA GAAGCATCCT GACATCTATG CGGTTCCAAT CAAAGCCCCC
    AAGCCAGATG CTGGCCTGCC CCAGCACATG AGACCCCCAG AGCCACAGAA AGCTCCTTCT
    AGGCTTTACC AGGACACCAG AGGAAGCTAC GGCAGTGATC CCGAGGAAGA GGAGGAGTAC
    CGCCAACAGT TGGCAGCACA CTCGAAGCGT GGTTACTACA GCCAGCCCTC CCGGTACCGA
    GACACCGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_446225.1
    CDS311..3805
    Translation

    Target ORF information:

    RefSeq Version NM_053773.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus tight junction protein 2 (Tjp2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_053773.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGGAGG TGATATGGGA ACAGTACACT GTGACCCTGC AGAAGGATTC CAAAAGAGGA 
    TTTGGAATCG CAGTGTCAGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACGTCA
    ATCGTCATTT CAGATGTACT CCCAGGTGGA CCCGCTGATG GCTTGCTTCA AGAAAATGAC
    AGGGTGGTCA TGGTCAATGG CACCCCCATG GAGGATGTGC TCCATTCATT TGCCGTTCAG
    CAGCTTAGGA AAAGTGGGAA GATCGCAGCC ATTGTGGTCA AGAGGCCTCG GAAGGTTCAG
    GTGGCCCCGC TGCAGGGCAG CCCCCCTCTC AGTCATGATG ACCGAGGTTT TGAGATGATG
    GATGAATTTG ATGGCAGAAG TTTCCGCAGC GGCTACAGTG AGAGGAGCCG GCACAGCAGC
    CACGATATGC TCAGCCACAG CTGGGAGGAC AGCAGGGAGA GGGGGCGTCC CCATCAGCGC
    GCACAGAGCC GGGAGCGGGA GCGCAGCCGT GGCCGGAGCC TGGAACGTGG CCTGGACCAT
    GAAGACTACG GGCGCAGCCA TGAGCGCAGC CGCGGCCGGA GCCTGGAGCG CGGTCTAGAC
    CGCGACTTTG TGTCCCGGGA CTACAGCCGT GGACGGAGCA TCGACAGGGA CTACGACCGA
    GACTATGAGC GCTCCTATCA CCAAGCCTAT GAGCCTGACT ACGAAGGCCG CTACAGCCCA
    TCTTATGACC GCAGAGCCCA TCCAGAGACC CGCTATGAAC GGAGCCGCAG CCGAGAACAC
    CTACGGTCCC GAAGCCCCAC CCCCGAGTCT AGGTCCCGGC AGGAACACAA GGGCCAGCAT
    GATCCCGACA GGCCCATCGG GGTCCTTCTG ACCAAAAGCA AAGCAAACGA AGAGTACGGG
    CTCCGGCTGG GAAGTCAGAT CTTCATTAAG GAAATGACCA GAACAGGACT GGCAACAAAA
    GATGGCAACC TTCATGAGGG GGACATAATT CTCAAGATCA ATGGAACTGT TACTGAGAAC
    ATGTCTTTAA CCGATGCTCG GAAGTTAATA GAAAAGTCTC GAGGAAAACT GCAGCTGGTG
    GTGTTGAGAG ACAGCAAGCA GACCCTCATC AACATCCCAT CTCTGAATGA CAGCGACTCG
    GAAGTGGAGG ATATCTCGGA AATCGAGTCC AACCGATCTT TTTCTCCAGA GGAGAGGCGC
    CAGCAGTATT CTGATCAGGA GTATCATTCC TCCACTGAGA AGCTGAAGGA GAGGCCAAGC
    TCAAGAGAGG AGACCTCAGG CAGAATGTCC AGGATGGGTG CCACACCCAC GCCGTTCAAG
    TCCACGGGGG ACATCACAGC TGCAGGTGTC ACAGAGGCCA ACAAGGAGCC CAGGTCCCAG
    GAGGAATCCC CAGTTCCTCA ACCCAGAACA GCATCAAGAG TCTTTCTTCG TCCTAGTCCC
    GAAGATGAAG CAATATATGG CCCTAACACC AAAATGGTGA AGTTCAAGAA GGGAGACAGC
    GTGGGCCTCC GGTTGGCTGG TGGAAATGAT GTTGGCATAT TTGTGGCTGG CATTCAGGAG
    GGCACCTCTG CAGAGCAGGA GGGCCTACAA GAAGGGGACC AGATTCTGAA GGTGAACACA
    CAAGATTTCA GAGGGCTGGT CCGGGAAGAT GCCGTCCTCT ACCTGTTAGA AATCCCTAAA
    GGTGAAACCG TGACCATTTT GGCTCAGAGC CGAGCAGACG TGTATAGAGA CATCCTGGCC
    TGTGGCAGGG GAGACTCGTT CTTCATAAGG AGCCACTTTG AATGTGAGAA GGAGACTCCG
    CAGAGCTTGG CCTTCACCAG GGGAGAGGTC TTCCGAGTGG TAGACACGCT GTACGATGGC
    AAACTGGGCC ACTGGCTGGC TGTGAGGATT GGAAATGAGC TGGAGAAGGG CTTAATCCCT
    AACAAAAGCA GAGCTGAGCA GATGGCCAGT GTCCAGAATG CCCAGCGAGA GAATGCTGGG
    GACAGAGCAG ACTTCTGGCG GATGCGTGGC CAGAGATCCG GGGTCAAGAA GAACATTCGC
    AAGAGCCGGG AAGACCTGGC AGCTGCTGTG TCGGTTAGCA CCAAGTTCCC CGCCTACGAG
    AAGGTTCTGC TTCGGGAAGC TGGCTTCAAG AGACCCGTGG TTCTGTTTGG CCCCATAGCA
    GATATAGCAA TGGAAAGGCT GACTACTGAG CTACCCGACC TGTTTCAAAC TGCAAAAACA
    GAACCCAAAG ATGCGGGATC TGAGAAATCC AGTGGAGTGG TTCGGTTGAA TACTGTGAGG
    CAAATTATTG AGCAGGACAA GCATGCCCTG CTCGACGTTA CCCCCAAAGC TGTGGACCTG
    CTCAATTATA CTCAGTGGTT CCCAATCGTG ATTTTCTTCA ACCCGGATTC CAGACAAGGC
    ATTAAAACCA TAAGGCAGAG GTTGAACCCA ACATCCAATA AAAGTTCTCG CAAGTTATTC
    GATCAAGCCA ACAAGCTCAA AAAAACCTGT TCTCATCTTT TTACAGCTAC CATCAACGTG
    AATTCAGCCA ATGATGGCTG GTTTGGCAGC CTGAAGGACA GCATTCAGCA GCAGCAAAAC
    GAAGCAGTGT GGGTCTCTGA AGGAAAGATG GAGGGGATGG ATGATGACGC TGAAGACCGC
    ATGTCCTACT TAACCGCCAT GGGTGCGGAC TATCTGAGTT GTGACAGCCG TCTCATCAGT
    GACTTTGAAG ATACGGACGG CGAGGGAGGC GCCTACACTG ACAATGAGCT GGATGAGCCA
    GCTGAGGAGC CGCTGGTGTC TTCCATCACC CGCTCCTCAG AGCCGGTGCA GCATGAGGAG
    AGCATAAGGA AGCCCAGCCC AGAGCCACGC GCTCAGATGA GGAGGGCAGC TAGCAGAGAC
    CAGCTTAGGG ATGGTAGCCC GCCCCCAGCA TTCAAGCCAG AGCCGCCCAA GGTCAGAAAC
    CAAAACAGAG AGGACTCTTT CAACTACTCC AAGTCAAACC TCTCTGCCAT GGCTGGCAGT
    GAAATCCCGG GGGGATCCAC CAAAGGGTGT CCTCCCCCTA TTGCGGTGAA ACCTGCCTTT
    GGGCGATCCA TCCTGAAGCC TTCTACTCCA GTCCCCATGC CTGAGAGTGA GGAGGTTGGG
    GAGAGCACCG AGGAGCAGGA AGAGGCTCCC AAATCAGTCC TGGGCAGAGT GAAAATCTTC
    GAGAAGATGG ACCACAAGGC GAAATTACAG AGGATGCAGG AGCTCCAAGA AGCACAGAAT
    GCGAGGATTG AAATAGCTCA GAAGCATCCT GACATCTATG CGGTTCCAAT CAAAGCCCCC
    AAGCCAGATG CTGGCCTGCC CCAGCACATG AGACCCCCAG AGCCACAGAA AGCTCCTTCT
    AGGCTTTACC AGGACACCAG AGGAAGCTAC GGCAGTGATC CCGAGGAAGA GGAGGAGTAC
    CGCCAACAGT TGGCAGCACA CTCGAAGCGT GGTTACTACA GCCAGCCCTC CCGGTACCGA
    GACACCGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa23971
    Clone ID Related Accession (Same CDS sequence) XM_006231175.3
    Accession Version XM_006231175.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3774bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product tight junction protein ZO-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231175.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGACTGT ATACCGAGAA AGACAAGATG AGATACAAGA AATACATGGC TATTCTGGAA 
    GCGGCAGTGG GCATTACCCC ACTTAACAAA AGAGAGCTTC TACCCGAGAG CCGGAGACAC
    GTAAACCTTT GGCAGCACCC GGGGGCCCGG AGTCCTGCCT TGCTCTCTTC TCCCTGTCAG
    TGGGACTGGC ACCCAGGATA CAGCTCCAGG CCCAAGCACT CTGCAGCGCA CAGACCGGTG
    TCCACCTACA GCCTGCTCTC CCAGGGCCCA GGCATGGAGG AGGTGATATG GGAACAGTAC
    ACTGTGACCC TGCAGAAGGA TTCCAAAAGA GGATTTGGAA TCGCAGTGTC AGGAGGCAGA
    GACAACCCCC ACTTTGAAAA TGGGGAGACG TCAATCGTCA TTTCAGATGT ACTCCCAGGT
    GGACCCGCTG ATGGCTTGCT TCAAGAAAAT GACAGGGTGG TCATGGTCAA TGGCACCCCC
    ATGGAGGATG TGCTCCATTC ATTTGCCGTT CAGCAGCTTA GGAAAAGTGG GAAGATCGCA
    GCCATTGTGG TCAAGAGGCC TCGGAAGGTT CAGGTGGCCC CGCTGCAGGG CAGCCCCCCT
    CTCAGTCATG ATGACCGAGG TTTTGAGATG ATGGATGAAT TTGATGGCAG AAGTTTCCGC
    AGCGGCTACA GTGAGAGGAG CCGGCACAGC AGCCACGATA TGCTCAGCCA CAGCTGGGAG
    GACAGCAGGG AGAGGGGGCG TCCCCATCAG CGCGCACAGA GCCGGGAGCG GGAGCGCAGC
    CGTGGCCGGA GCCTGGAACG TGGCCTGGAC CATGAAGACT ACGGGCGCAG CCATGAGCGC
    AGCCGCGGCC GGAGCCTGGA GCGCGGTCTA GACCGCGACT TTGTGTCCCG GGACTACAGC
    CGTGGACGGA GCATCGACAG GGACTACGAC CGAGACTATG AGCGCTCCTA TCACCAAGCC
    TATGAGCCTG ACTACGAAGG CCGCTACAGC CCATCTTATG ACCGCAGAGC CCATCCAGAG
    ACCCGCTATG AACGGAGCCG CAGCCGAGAA CACCTACGGT CCCGAAGCCC CACCCCCGAG
    TCTAGGTCCC GGCAGGAACA CAAGGGCCAG CATGATCCCG ACAGGCCCAT CGGGGTCCTT
    CTGACCAAAA GCAAAGCAAA CGAAGAGTAC GGGCTCCGGC TGGGAAGTCA GATCTTCATT
    AAGGAAATGA CCAGAACAGG ACTGGCAACA AAAGATGGCA ACCTTCATGA GGGGGACATA
    ATTCTCAAGA TCAATGGAAC TGTTACTGAG AACATGTCTT TAACCGATGC TCGGAAGTTA
    ATAGAAAAGT CTCGAGGAAA ACTGCAGCTG GTGGTGTTGA GAGACAGCAA GCAGACCCTC
    ATCAACATCC CATCTCTGAA TGACAGCGAC TCGGAAGTGG AGGATATCTC GGAAATCGAG
    TCCAACCGAT CTTTTTCTCC AGAGGAGAGG CGCCAGCAGT ATTCTGATCA GGAGTATCAT
    TCCTCCACTG AGAAGCTGAA GGAGAGGCCA AGCTCAAGAG AGGAGACCTC AGGCAGAATG
    TCCAGGATGG GTGCCACACC CACGCCGTTC AAGTCCACGG GGGACATCAC AGCTGCAGGT
    GTCACAGAGG CCAACAAGGA GCCCAGGTCC CAGGAGGAAT CCCCAGTTCC TCAACCCAGA
    ACAGCATCAA GAGTCTTTCT TCGTCCTAGT CCCGAAGATG AAGCAATATA TGGCCCTAAC
    ACCAAAATGG TGAAGTTCAA GAAGGGAGAC AGCGTGGGCC TCCGGTTGGC TGGTGGAAAT
    GATGTTGGCA TATTTGTGGC TGGCATTCAG GAGGGCACCT CTGCAGAGCA GGAGGGCCTA
    CAAGAAGGGG ACCAGATTCT GAAGGTGAAC ACACAAGATT TCAGAGGGCT GGTCCGGGAA
    GATGCCGTCC TCTACCTGTT AGAAATCCCT AAAGGTGAAA CCGTGACCAT TTTGGCTCAG
    AGCCGAGCAG ACGTGTATAG AGACATCCTG GCCTGTGGCA GGGGAGACTC GTTCTTCATA
    AGGAGCCACT TTGAATGTGA GAAGGAGACT CCGCAGAGCT TGGCCTTCAC CAGGGGAGAG
    GTCTTCCGAG TGGTAGACAC GCTGTACGAT GGCAAACTGG GCCACTGGCT GGCTGTGAGG
    ATTGGAAATG AGCTGGAGAA GGGCTTAATC CCTAACAAAA GCAGAGCTGA GCAGATGGCC
    AGTGTCCAGA ATGCCCAGCG AGAGAATGCT GGGGACAGAG CAGACTTCTG GCGGATGCGT
    GGCCAGAGAT CCGGGGTCAA GAAGAACATT CGCAAGAGCC GGGAAGACCT GGCAGCTGCT
    GTGTCGGTTA GCACCAAGTT CCCCGCCTAC GAGAAGGTTC TGCTTCGGGA AGCTGGCTTC
    AAGAGACCCG TGGTTCTGTT TGGCCCCATA GCAGATATAG CAATGGAAAG GCTGACTACT
    GAGCTACCCG ACCTGTTTCA AACTGCAAAA ACAGAACCCA AAGATGCGGG ATCTGAGAAA
    TCCAGTGGAG TGGTTCGGTT GAATACTGTG AGGCAAATTA TTGAGCAGGA CAAGCATGCC
    CTGCTCGACG TTACCCCCAA AGCTGTGGAC CTGCTCAATT ATACTCAGTG GTTCCCAATC
    GTGATTTTCT TCAACCCGGA TTCCAGACAA GGCATTAAAA CCATAAGGCA GAGGTTGAAC
    CCAACATCCA ATAAAAGTTC TCGCAAGTTA TTCGATCAAG CCAACAAGCT CAAAAAAACC
    TGTTCTCATC TTTTTACAGC TACCATCAAC GTGAATTCAG CCAATGATGG CTGGTTTGGC
    AGCCTGAAGG ACAGCATTCA GCAGCAGCAA AACGAAGCAG TGTGGGTCTC TGAAGGAAAG
    ATGGAGGGGA TGGATGATGA CGCTGAAGAC CGCATGTCCT ACTTAACCGC CATGGGTGCG
    GACTATCTGA GTTGTGACAG CCGTCTCATC AGTGACTTTG AAGATACGGA CGGCGAGGGA
    GGCGCCTACA CTGACAATGA GCTGGATGAG CCAGCTGAGG AGCCGCTGGT GTCTTCCATC
    ACCCGCTCCT CAGAGCCGGT GCAGCATGAG GAGAGCATAA GGAAGCCCAG CCCAGAGCCA
    CGCGCTCAGA TGAGGAGGGC AGCTAGCAGA GACCAGCTTA GGGATGGTAG CCCGCCCCCA
    GCATTCAAGC CAGAGCCGCC CAAGGTCAGA AACCAAAACA GAGAGGACTC TTTCAACTAC
    TCCAAGTCAA ACCTCTCTGC CATGGCTGGC AGTGAAATCC CGGGGGGATC CACCAAAGGG
    TGTCCTCCCC CTATTGCGGT GAAACCTGCC TTTGGGCGAT CCATCCTGAA GCCTTCTACT
    CCAGTCCCCA TGCCTGAGAG TGAGGAGGTT GGGGAGAGCA CCGAGGAGCA GGAAGAGGCT
    CCCAAATCAG TCCTGGGCAG AGTGAAAATC TTCGAGAAGA TGGACCACAA GGCGAAATTA
    CAGAGGATGC AGGAGCTCCA AGAAGCACAG AATGCGAGGA TTGAAATAGC TCAGAAGCAT
    CCTGACATCT ATGCGGTTCC AATCAAAGCC CCCAAGCCAG ATGCTGGCCT GCCCCAGCAC
    ATGAGTTCTA GACCCCCAGA GCCACAGAAA GCTCCTTCTA GGCTTTACCA GGACACCAGA
    GGAAGCTACG GCAGTGATCC CGAGGAAGAG GAGGAGTACC GCCAACAGTT GGCAGCACAC
    TCGAAGCGTG GTTACTACAG CCAGCCCTCC CGGTACCGAG ACACCGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006231237.1
    CDS555..4328
    Translation

    Target ORF information:

    RefSeq Version XM_006231175.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006231175.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGACTGT ATACCGAGAA AGACAAGATG AGATACAAGA AATACATGGC TATTCTGGAA 
    GCGGCAGTGG GCATTACCCC ACTTAACAAA AGAGAGCTTC TACCCGAGAG CCGGAGACAC
    GTAAACCTTT GGCAGCACCC GGGGGCCCGG AGTCCTGCCT TGCTCTCTTC TCCCTGTCAG
    TGGGACTGGC ACCCAGGATA CAGCTCCAGG CCCAAGCACT CTGCAGCGCA CAGACCGGTG
    TCCACCTACA GCCTGCTCTC CCAGGGCCCA GGCATGGAGG AGGTGATATG GGAACAGTAC
    ACTGTGACCC TGCAGAAGGA TTCCAAAAGA GGATTTGGAA TCGCAGTGTC AGGAGGCAGA
    GACAACCCCC ACTTTGAAAA TGGGGAGACG TCAATCGTCA TTTCAGATGT ACTCCCAGGT
    GGACCCGCTG ATGGCTTGCT TCAAGAAAAT GACAGGGTGG TCATGGTCAA TGGCACCCCC
    ATGGAGGATG TGCTCCATTC ATTTGCCGTT CAGCAGCTTA GGAAAAGTGG GAAGATCGCA
    GCCATTGTGG TCAAGAGGCC TCGGAAGGTT CAGGTGGCCC CGCTGCAGGG CAGCCCCCCT
    CTCAGTCATG ATGACCGAGG TTTTGAGATG ATGGATGAAT TTGATGGCAG AAGTTTCCGC
    AGCGGCTACA GTGAGAGGAG CCGGCACAGC AGCCACGATA TGCTCAGCCA CAGCTGGGAG
    GACAGCAGGG AGAGGGGGCG TCCCCATCAG CGCGCACAGA GCCGGGAGCG GGAGCGCAGC
    CGTGGCCGGA GCCTGGAACG TGGCCTGGAC CATGAAGACT ACGGGCGCAG CCATGAGCGC
    AGCCGCGGCC GGAGCCTGGA GCGCGGTCTA GACCGCGACT TTGTGTCCCG GGACTACAGC
    CGTGGACGGA GCATCGACAG GGACTACGAC CGAGACTATG AGCGCTCCTA TCACCAAGCC
    TATGAGCCTG ACTACGAAGG CCGCTACAGC CCATCTTATG ACCGCAGAGC CCATCCAGAG
    ACCCGCTATG AACGGAGCCG CAGCCGAGAA CACCTACGGT CCCGAAGCCC CACCCCCGAG
    TCTAGGTCCC GGCAGGAACA CAAGGGCCAG CATGATCCCG ACAGGCCCAT CGGGGTCCTT
    CTGACCAAAA GCAAAGCAAA CGAAGAGTAC GGGCTCCGGC TGGGAAGTCA GATCTTCATT
    AAGGAAATGA CCAGAACAGG ACTGGCAACA AAAGATGGCA ACCTTCATGA GGGGGACATA
    ATTCTCAAGA TCAATGGAAC TGTTACTGAG AACATGTCTT TAACCGATGC TCGGAAGTTA
    ATAGAAAAGT CTCGAGGAAA ACTGCAGCTG GTGGTGTTGA GAGACAGCAA GCAGACCCTC
    ATCAACATCC CATCTCTGAA TGACAGCGAC TCGGAAGTGG AGGATATCTC GGAAATCGAG
    TCCAACCGAT CTTTTTCTCC AGAGGAGAGG CGCCAGCAGT ATTCTGATCA GGAGTATCAT
    TCCTCCACTG AGAAGCTGAA GGAGAGGCCA AGCTCAAGAG AGGAGACCTC AGGCAGAATG
    TCCAGGATGG GTGCCACACC CACGCCGTTC AAGTCCACGG GGGACATCAC AGCTGCAGGT
    GTCACAGAGG CCAACAAGGA GCCCAGGTCC CAGGAGGAAT CCCCAGTTCC TCAACCCAGA
    ACAGCATCAA GAGTCTTTCT TCGTCCTAGT CCCGAAGATG AAGCAATATA TGGCCCTAAC
    ACCAAAATGG TGAAGTTCAA GAAGGGAGAC AGCGTGGGCC TCCGGTTGGC TGGTGGAAAT
    GATGTTGGCA TATTTGTGGC TGGCATTCAG GAGGGCACCT CTGCAGAGCA GGAGGGCCTA
    CAAGAAGGGG ACCAGATTCT GAAGGTGAAC ACACAAGATT TCAGAGGGCT GGTCCGGGAA
    GATGCCGTCC TCTACCTGTT AGAAATCCCT AAAGGTGAAA CCGTGACCAT TTTGGCTCAG
    AGCCGAGCAG ACGTGTATAG AGACATCCTG GCCTGTGGCA GGGGAGACTC GTTCTTCATA
    AGGAGCCACT TTGAATGTGA GAAGGAGACT CCGCAGAGCT TGGCCTTCAC CAGGGGAGAG
    GTCTTCCGAG TGGTAGACAC GCTGTACGAT GGCAAACTGG GCCACTGGCT GGCTGTGAGG
    ATTGGAAATG AGCTGGAGAA GGGCTTAATC CCTAACAAAA GCAGAGCTGA GCAGATGGCC
    AGTGTCCAGA ATGCCCAGCG AGAGAATGCT GGGGACAGAG CAGACTTCTG GCGGATGCGT
    GGCCAGAGAT CCGGGGTCAA GAAGAACATT CGCAAGAGCC GGGAAGACCT GGCAGCTGCT
    GTGTCGGTTA GCACCAAGTT CCCCGCCTAC GAGAAGGTTC TGCTTCGGGA AGCTGGCTTC
    AAGAGACCCG TGGTTCTGTT TGGCCCCATA GCAGATATAG CAATGGAAAG GCTGACTACT
    GAGCTACCCG ACCTGTTTCA AACTGCAAAA ACAGAACCCA AAGATGCGGG ATCTGAGAAA
    TCCAGTGGAG TGGTTCGGTT GAATACTGTG AGGCAAATTA TTGAGCAGGA CAAGCATGCC
    CTGCTCGACG TTACCCCCAA AGCTGTGGAC CTGCTCAATT ATACTCAGTG GTTCCCAATC
    GTGATTTTCT TCAACCCGGA TTCCAGACAA GGCATTAAAA CCATAAGGCA GAGGTTGAAC
    CCAACATCCA ATAAAAGTTC TCGCAAGTTA TTCGATCAAG CCAACAAGCT CAAAAAAACC
    TGTTCTCATC TTTTTACAGC TACCATCAAC GTGAATTCAG CCAATGATGG CTGGTTTGGC
    AGCCTGAAGG ACAGCATTCA GCAGCAGCAA AACGAAGCAG TGTGGGTCTC TGAAGGAAAG
    ATGGAGGGGA TGGATGATGA CGCTGAAGAC CGCATGTCCT ACTTAACCGC CATGGGTGCG
    GACTATCTGA GTTGTGACAG CCGTCTCATC AGTGACTTTG AAGATACGGA CGGCGAGGGA
    GGCGCCTACA CTGACAATGA GCTGGATGAG CCAGCTGAGG AGCCGCTGGT GTCTTCCATC
    ACCCGCTCCT CAGAGCCGGT GCAGCATGAG GAGAGCATAA GGAAGCCCAG CCCAGAGCCA
    CGCGCTCAGA TGAGGAGGGC AGCTAGCAGA GACCAGCTTA GGGATGGTAG CCCGCCCCCA
    GCATTCAAGC CAGAGCCGCC CAAGGTCAGA AACCAAAACA GAGAGGACTC TTTCAACTAC
    TCCAAGTCAA ACCTCTCTGC CATGGCTGGC AGTGAAATCC CGGGGGGATC CACCAAAGGG
    TGTCCTCCCC CTATTGCGGT GAAACCTGCC TTTGGGCGAT CCATCCTGAA GCCTTCTACT
    CCAGTCCCCA TGCCTGAGAG TGAGGAGGTT GGGGAGAGCA CCGAGGAGCA GGAAGAGGCT
    CCCAAATCAG TCCTGGGCAG AGTGAAAATC TTCGAGAAGA TGGACCACAA GGCGAAATTA
    CAGAGGATGC AGGAGCTCCA AGAAGCACAG AATGCGAGGA TTGAAATAGC TCAGAAGCAT
    CCTGACATCT ATGCGGTTCC AATCAAAGCC CCCAAGCCAG ATGCTGGCCT GCCCCAGCAC
    ATGAGTTCTA GACCCCCAGA GCCACAGAAA GCTCCTTCTA GGCTTTACCA GGACACCAGA
    GGAAGCTACG GCAGTGATCC CGAGGAAGAG GAGGAGTACC GCCAACAGTT GGCAGCACAC
    TCGAAGCGTG GTTACTACAG CCAGCCCTCC CGGTACCGAG ACACCGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa23972
    Clone ID Related Accession (Same CDS sequence) XM_006231176.3
    Accession Version XM_006231176.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3663bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product tight junction protein ZO-2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231176.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGACTGT ATACCGAGAA AGACAAGATG AGATACAAGA AATACATGGC TATTCTGGAA 
    GCGGCAGTGG GCATTACCCC ACTTAACAAA AGAGAGCTTC TACCCGAGAG CCGGAGACAC
    GTAAACCTTT GGCAGCACCC GGGGGCCCGG AGTCCTGCCT TGCTCTCTTC TCCCTGTCAG
    TGGGACTGGC ACCCAGGATA CAGCTCCAGG CCCAAGCACT CTGCAGCGCA CAGACCGGTG
    TCCACCTACA GCCTGCTCTC CCAGGGCCCA GGCATGGAGG AGGTGATATG GGAACAGTAC
    ACTGTGACCC TGCAGAAGGA TTCCAAAAGA GGATTTGGAA TCGCAGTGTC AGGAGGCAGA
    GACAACCCCC ACTTTGAAAA TGGGGAGACG TCAATCGTCA TTTCAGATGT ACTCCCAGGT
    GGACCCGCTG ATGGCTTGCT TCAAGAAAAT GACAGGGTGG TCATGGTCAA TGGCACCCCC
    ATGGAGGATG TGCTCCATTC ATTTGCCGTT CAGCAGCTTA GGAAAAGTGG GAAGATCGCA
    GCCATTGTGG TCAAGAGGCC TCGGAAGGTT CAGGTGGCCC CGCTGCAGGG CAGCCCCCCT
    CTCAGTCATG ATGACCGAGG TTTTGAGATG ATGGATGAAT TTGATGGCAG AAGTTTCCGC
    AGCGGCTACA GTGAGAGGAG CCGGCACAGC AGCCACGATA TGCTCAGCCA CAGCTGGGAG
    GACAGCAGGG AGAGGGGGCG TCCCCATCAG CGCGCACAGA GCCGGGAGCG GGAGCGCAGC
    CGTGGCCGGA GCCTGGAACG TGGCCTGGAC CATGAAGACT ACGGGCGCAG CCATGAGCGC
    AGCCGCGGCC GGAGCCTGGA GCGCGGTCTA GACCGCGACT TTGTGTCCCG GGACTACAGC
    CGTGGACGGA GCATCGACAG GGACTACGAC CGAGACTATG AGCGCTCCTA TCACCAAGCC
    TATGAGCCTG ACTACGAAGG CCGCTACAGC CCATCTTATG ACCGCAGAGC CCATCCAGAG
    ACCCGCTATG AACGGAGCCG CAGCCGAGAA CACCTACGGT CCCGAAGCCC CACCCCCGAG
    TCTAGGTCCC GGCAGGAACA CAAGGGCCAG CATGATCCCG ACAGGCCCAT CGGGGTCCTT
    CTGACCAAAA GCAAAGCAAA CGAAGAGTAC GGGCTCCGGC TGGGAAGTCA GATCTTCATT
    AAGGAAATGA CCAGAACAGG ACTGGCAACA AAAGATGGCA ACCTTCATGA GGGGGACATA
    ATTCTCAAGA TCAATGGAAC TGTTACTGAG AACATGTCTT TAACCGATGC TCGGAAGTTA
    ATAGAAAAGT CTCGAGGAAA ACTGCAGCTG GTGGTGTTGA GAGACAGCAA GCAGACCCTC
    ATCAACATCC CATCTCTGAA TGACAGCGAC TCGGAAGTGG AGGATATCTC GGAAATCGAG
    TCCAACCGAT CTTTTTCTCC AGAGGAGAGG CGCCAGCAGT ATTCTGATCA GGAGTATCAT
    TCCTCCACTG AGAAGCTGAA GGAGAGGCCA AGCTCAAGAG AGGAGACCTC AGGCAGAATG
    TCCAGGATGG GTGCCACACC CACGCCGTTC AAGTCCACGG GGGACATCAC AGCTGCAGGT
    GTCACAGAGG CCAACAAGGA GCCCAGGTCC CAGGAGGAAT CCCCAGTTCC TCAACCCAGA
    ACAGCATCAA GAGTCTTTCT TCGTCCTAGT CCCGAAGATG AAGCAATATA TGGCCCTAAC
    ACCAAAATGG TGAAGTTCAA GAAGGGAGAC AGCGTGGGCC TCCGGTTGGC TGGTGGAAAT
    GATGTTGGCA TATTTGTGGC TGGCATTCAG GAGGGCACCT CTGCAGAGCA GGAGGGCCTA
    CAAGAAGGGG ACCAGATTCT GAAGGTGAAC ACACAAGATT TCAGAGGGCT GGTCCGGGAA
    GATGCCGTCC TCTACCTGTT AGAAATCCCT AAAGGTGAAA CCGTGACCAT TTTGGCTCAG
    AGCCGAGCAG ACGTGTATAG AGACATCCTG GCCTGTGGCA GGGGAGACTC GTTCTTCATA
    AGGAGCCACT TTGAATGTGA GAAGGAGACT CCGCAGAGCT TGGCCTTCAC CAGGGGAGAG
    GTCTTCCGAG TGGTAGACAC GCTGTACGAT GGCAAACTGG GCCACTGGCT GGCTGTGAGG
    ATTGGAAATG AGCTGGAGAA GGGCTTAATC CCTAACAAAA GCAGAGCTGA GCAGATGGCC
    AGTGTCCAGA ATGCCCAGCG AGAGAATGCT GGGGACAGAG CAGACTTCTG GCGGATGCGT
    GGCCAGAGAT CCGGGGTCAA GAAGAACATT CGCAAGAGCC GGGAAGACCT GGCAGCTGCT
    GTGTCGGTTA GCACCAAGTT CCCCGCCTAC GAGAAGGTTC TGCTTCGGGA AGCTGGCTTC
    AAGAGACCCG TGGTTCTGTT TGGCCCCATA GCAGATATAG CAATGGAAAG GCTGACTACT
    GAGCTACCCG ACCTGTTTCA AACTGCAAAA ACAGAACCCA AAGATGCGGG ATCTGAGAAA
    TCCAGTGGAG TGGTTCGGTT GAATACTGTG AGGCAAATTA TTGAGCAGGA CAAGCATGCC
    CTGCTCGACG TTACCCCCAA AGCTGTGGAC CTGCTCAATT ATACTCAGTG GTTCCCAATC
    GTGATTTTCT TCAACCCGGA TTCCAGACAA GGCATTAAAA CCATAAGGCA GAGGTTGAAC
    CCAACATCCA ATAAAAGTTC TCGCAAGTTA TTCGATCAAG CCAACAAGCT CAAAAAAACC
    TGTTCTCATC TTTTTACAGC TACCATCAAC GTGAATTCAG CCAATGATGG CTGGTTTGGC
    AGCCTGAAGG ACAGCATTCA GCAGCAGCAA AACGAAGCAG TGTGGGTCTC TGAAGGAAAG
    ATGGAGGGGA TGGATGATGA CGCTGAAGAC CGCATGTCCT ACTTAACCGC CATGGGTGCG
    GACTATCTGA GTTGTGACAG CCGTCTCATC AGTGACTTTG AAGATACGGA CGGCGAGGGA
    GGCGCCTACA CTGACAATGA GCTGGATGAG CCAGCTGAGG AGCCGCTGGT GTCTTCCATC
    ACCCGCTCCT CAGAGCCGGT GCAGCATGAG GAGGTCAGAA ACCAAAACAG AGAGGACTCT
    TTCAACTACT CCAAGTCAAA CCTCTCTGCC ATGGCTGGCA GTGAAATCCC GGGGGGATCC
    ACCAAAGGGT GTCCTCCCCC TATTGCGGTG AAACCTGCCT TTGGGCGATC CATCCTGAAG
    CCTTCTACTC CAGTCCCCAT GCCTGAGAGT GAGGAGGTTG GGGAGAGCAC CGAGGAGCAG
    GAAGAGGCTC CCAAATCAGT CCTGGGCAGA GTGAAAATCT TCGAGAAGAT GGACCACAAG
    GCGAAATTAC AGAGGATGCA GGAGCTCCAA GAAGCACAGA ATGCGAGGAT TGAAATAGCT
    CAGAAGCATC CTGACATCTA TGCGGTTCCA ATCAAAGCCC CCAAGCCAGA TGCTGGCCTG
    CCCCAGCACA TGAGTTCTAG ACCCCCAGAG CCACAGAAAG CTCCTTCTAG GCTTTACCAG
    GACACCAGAG GAAGCTACGG CAGTGATCCC GAGGAAGAGG AGGAGTACCG CCAACAGTTG
    GCAGCACACT CGAAGCGTGG TTACTACAGC CAGCCCTCCC GGTACCGAGA CACCGAATTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006231238.1
    CDS555..4217
    Translation

    Target ORF information:

    RefSeq Version XM_006231176.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006231176.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGACTGT ATACCGAGAA AGACAAGATG AGATACAAGA AATACATGGC TATTCTGGAA 
    GCGGCAGTGG GCATTACCCC ACTTAACAAA AGAGAGCTTC TACCCGAGAG CCGGAGACAC
    GTAAACCTTT GGCAGCACCC GGGGGCCCGG AGTCCTGCCT TGCTCTCTTC TCCCTGTCAG
    TGGGACTGGC ACCCAGGATA CAGCTCCAGG CCCAAGCACT CTGCAGCGCA CAGACCGGTG
    TCCACCTACA GCCTGCTCTC CCAGGGCCCA GGCATGGAGG AGGTGATATG GGAACAGTAC
    ACTGTGACCC TGCAGAAGGA TTCCAAAAGA GGATTTGGAA TCGCAGTGTC AGGAGGCAGA
    GACAACCCCC ACTTTGAAAA TGGGGAGACG TCAATCGTCA TTTCAGATGT ACTCCCAGGT
    GGACCCGCTG ATGGCTTGCT TCAAGAAAAT GACAGGGTGG TCATGGTCAA TGGCACCCCC
    ATGGAGGATG TGCTCCATTC ATTTGCCGTT CAGCAGCTTA GGAAAAGTGG GAAGATCGCA
    GCCATTGTGG TCAAGAGGCC TCGGAAGGTT CAGGTGGCCC CGCTGCAGGG CAGCCCCCCT
    CTCAGTCATG ATGACCGAGG TTTTGAGATG ATGGATGAAT TTGATGGCAG AAGTTTCCGC
    AGCGGCTACA GTGAGAGGAG CCGGCACAGC AGCCACGATA TGCTCAGCCA CAGCTGGGAG
    GACAGCAGGG AGAGGGGGCG TCCCCATCAG CGCGCACAGA GCCGGGAGCG GGAGCGCAGC
    CGTGGCCGGA GCCTGGAACG TGGCCTGGAC CATGAAGACT ACGGGCGCAG CCATGAGCGC
    AGCCGCGGCC GGAGCCTGGA GCGCGGTCTA GACCGCGACT TTGTGTCCCG GGACTACAGC
    CGTGGACGGA GCATCGACAG GGACTACGAC CGAGACTATG AGCGCTCCTA TCACCAAGCC
    TATGAGCCTG ACTACGAAGG CCGCTACAGC CCATCTTATG ACCGCAGAGC CCATCCAGAG
    ACCCGCTATG AACGGAGCCG CAGCCGAGAA CACCTACGGT CCCGAAGCCC CACCCCCGAG
    TCTAGGTCCC GGCAGGAACA CAAGGGCCAG CATGATCCCG ACAGGCCCAT CGGGGTCCTT
    CTGACCAAAA GCAAAGCAAA CGAAGAGTAC GGGCTCCGGC TGGGAAGTCA GATCTTCATT
    AAGGAAATGA CCAGAACAGG ACTGGCAACA AAAGATGGCA ACCTTCATGA GGGGGACATA
    ATTCTCAAGA TCAATGGAAC TGTTACTGAG AACATGTCTT TAACCGATGC TCGGAAGTTA
    ATAGAAAAGT CTCGAGGAAA ACTGCAGCTG GTGGTGTTGA GAGACAGCAA GCAGACCCTC
    ATCAACATCC CATCTCTGAA TGACAGCGAC TCGGAAGTGG AGGATATCTC GGAAATCGAG
    TCCAACCGAT CTTTTTCTCC AGAGGAGAGG CGCCAGCAGT ATTCTGATCA GGAGTATCAT
    TCCTCCACTG AGAAGCTGAA GGAGAGGCCA AGCTCAAGAG AGGAGACCTC AGGCAGAATG
    TCCAGGATGG GTGCCACACC CACGCCGTTC AAGTCCACGG GGGACATCAC AGCTGCAGGT
    GTCACAGAGG CCAACAAGGA GCCCAGGTCC CAGGAGGAAT CCCCAGTTCC TCAACCCAGA
    ACAGCATCAA GAGTCTTTCT TCGTCCTAGT CCCGAAGATG AAGCAATATA TGGCCCTAAC
    ACCAAAATGG TGAAGTTCAA GAAGGGAGAC AGCGTGGGCC TCCGGTTGGC TGGTGGAAAT
    GATGTTGGCA TATTTGTGGC TGGCATTCAG GAGGGCACCT CTGCAGAGCA GGAGGGCCTA
    CAAGAAGGGG ACCAGATTCT GAAGGTGAAC ACACAAGATT TCAGAGGGCT GGTCCGGGAA
    GATGCCGTCC TCTACCTGTT AGAAATCCCT AAAGGTGAAA CCGTGACCAT TTTGGCTCAG
    AGCCGAGCAG ACGTGTATAG AGACATCCTG GCCTGTGGCA GGGGAGACTC GTTCTTCATA
    AGGAGCCACT TTGAATGTGA GAAGGAGACT CCGCAGAGCT TGGCCTTCAC CAGGGGAGAG
    GTCTTCCGAG TGGTAGACAC GCTGTACGAT GGCAAACTGG GCCACTGGCT GGCTGTGAGG
    ATTGGAAATG AGCTGGAGAA GGGCTTAATC CCTAACAAAA GCAGAGCTGA GCAGATGGCC
    AGTGTCCAGA ATGCCCAGCG AGAGAATGCT GGGGACAGAG CAGACTTCTG GCGGATGCGT
    GGCCAGAGAT CCGGGGTCAA GAAGAACATT CGCAAGAGCC GGGAAGACCT GGCAGCTGCT
    GTGTCGGTTA GCACCAAGTT CCCCGCCTAC GAGAAGGTTC TGCTTCGGGA AGCTGGCTTC
    AAGAGACCCG TGGTTCTGTT TGGCCCCATA GCAGATATAG CAATGGAAAG GCTGACTACT
    GAGCTACCCG ACCTGTTTCA AACTGCAAAA ACAGAACCCA AAGATGCGGG ATCTGAGAAA
    TCCAGTGGAG TGGTTCGGTT GAATACTGTG AGGCAAATTA TTGAGCAGGA CAAGCATGCC
    CTGCTCGACG TTACCCCCAA AGCTGTGGAC CTGCTCAATT ATACTCAGTG GTTCCCAATC
    GTGATTTTCT TCAACCCGGA TTCCAGACAA GGCATTAAAA CCATAAGGCA GAGGTTGAAC
    CCAACATCCA ATAAAAGTTC TCGCAAGTTA TTCGATCAAG CCAACAAGCT CAAAAAAACC
    TGTTCTCATC TTTTTACAGC TACCATCAAC GTGAATTCAG CCAATGATGG CTGGTTTGGC
    AGCCTGAAGG ACAGCATTCA GCAGCAGCAA AACGAAGCAG TGTGGGTCTC TGAAGGAAAG
    ATGGAGGGGA TGGATGATGA CGCTGAAGAC CGCATGTCCT ACTTAACCGC CATGGGTGCG
    GACTATCTGA GTTGTGACAG CCGTCTCATC AGTGACTTTG AAGATACGGA CGGCGAGGGA
    GGCGCCTACA CTGACAATGA GCTGGATGAG CCAGCTGAGG AGCCGCTGGT GTCTTCCATC
    ACCCGCTCCT CAGAGCCGGT GCAGCATGAG GAGGTCAGAA ACCAAAACAG AGAGGACTCT
    TTCAACTACT CCAAGTCAAA CCTCTCTGCC ATGGCTGGCA GTGAAATCCC GGGGGGATCC
    ACCAAAGGGT GTCCTCCCCC TATTGCGGTG AAACCTGCCT TTGGGCGATC CATCCTGAAG
    CCTTCTACTC CAGTCCCCAT GCCTGAGAGT GAGGAGGTTG GGGAGAGCAC CGAGGAGCAG
    GAAGAGGCTC CCAAATCAGT CCTGGGCAGA GTGAAAATCT TCGAGAAGAT GGACCACAAG
    GCGAAATTAC AGAGGATGCA GGAGCTCCAA GAAGCACAGA ATGCGAGGAT TGAAATAGCT
    CAGAAGCATC CTGACATCTA TGCGGTTCCA ATCAAAGCCC CCAAGCCAGA TGCTGGCCTG
    CCCCAGCACA TGAGTTCTAG ACCCCCAGAG CCACAGAAAG CTCCTTCTAG GCTTTACCAG
    GACACCAGAG GAAGCTACGG CAGTGATCCC GAGGAAGAGG AGGAGTACCG CCAACAGTTG
    GCAGCACACT CGAAGCGTGG TTACTACAGC CAGCCCTCCC GGTACCGAGA CACCGAATTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa23973
    Clone ID Related Accession (Same CDS sequence) XM_006231177.3
    Accession Version XM_006231177.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product tight junction protein ZO-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231177.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGGGCTG CTCAGGTCTT CCAGAGGATG TGGATCCAGG CAGTTAAAAA GTTGGAGAGA 
    TGGAAAGGCC ATGGCCCAGG CATGGAGGAG GTGATATGGG AACAGTACAC TGTGACCCTG
    CAGAAGGATT CCAAAAGAGG ATTTGGAATC GCAGTGTCAG GAGGCAGAGA CAACCCCCAC
    TTTGAAAATG GGGAGACGTC AATCGTCATT TCAGATGTAC TCCCAGGTGG ACCCGCTGAT
    GGCTTGCTTC AAGAAAATGA CAGGGTGGTC ATGGTCAATG GCACCCCCAT GGAGGATGTG
    CTCCATTCAT TTGCCGTTCA GCAGCTTAGG AAAAGTGGGA AGATCGCAGC CATTGTGGTC
    AAGAGGCCTC GGAAGGTTCA GGTGGCCCCG CTGCAGGGCA GCCCCCCTCT CAGTCATGAT
    GACCGAGGTT TTGAGATGAT GGATGAATTT GATGGCAGAA GTTTCCGCAG CGGCTACAGT
    GAGAGGAGCC GGCACAGCAG CCACGATATG CTCAGCCACA GCTGGGAGGA CAGCAGGGAG
    AGGGGGCGTC CCCATCAGCG CGCACAGAGC CGGGAGCGGG AGCGCAGCCG TGGCCGGAGC
    CTGGAACGTG GCCTGGACCA TGAAGACTAC GGGCGCAGCC ATGAGCGCAG CCGCGGCCGG
    AGCCTGGAGC GCGGTCTAGA CCGCGACTTT GTGTCCCGGG ACTACAGCCG TGGACGGAGC
    ATCGACAGGG ACTACGACCG AGACTATGAG CGCTCCTATC ACCAAGCCTA TGAGCCTGAC
    TACGAAGGCC GCTACAGCCC ATCTTATGAC CGCAGAGCCC ATCCAGAGAC CCGCTATGAA
    CGGAGCCGCA GCCGAGAACA CCTACGGTCC CGAAGCCCCA CCCCCGAGTC TAGGTCCCGG
    CAGGAACACA AGGGCCAGCA TGATCCCGAC AGGCCCATCG GGGTCCTTCT GACCAAAAGC
    AAAGCAAACG AAGAGTACGG GCTCCGGCTG GGAAGTCAGA TCTTCATTAA GGAAATGACC
    AGAACAGGAC TGGCAACAAA AGATGGCAAC CTTCATGAGG GGGACATAAT TCTCAAGATC
    AATGGAACTG TTACTGAGAA CATGTCTTTA ACCGATGCTC GGAAGTTAAT AGAAAAGTCT
    CGAGGAAAAC TGCAGCTGGT GGTGTTGAGA GACAGCAAGC AGACCCTCAT CAACATCCCA
    TCTCTGAATG ACAGCGACTC GGAAGTGGAG GATATCTCGG AAATCGAGTC CAACCGATCT
    TTTTCTCCAG AGGAGAGGCG CCAGCAGTAT TCTGATCAGG AGTATCATTC CTCCACTGAG
    AAGCTGAAGG AGAGGCCAAG CTCAAGAGAG GAGACCTCAG GCAGAATGTC CAGGATGGGT
    GCCACACCCA CGCCGTTCAA GTCCACGGGG GACATCACAG CTGCAGGTGT CACAGAGGCC
    AACAAGGAGC CCAGGTCCCA GGAGGAATCC CCAGTTCCTC AACCCAGAAC AGCATCAAGA
    GTCTTTCTTC GTCCTAGTCC CGAAGATGAA GCAATATATG GCCCTAACAC CAAAATGGTG
    AAGTTCAAGA AGGGAGACAG CGTGGGCCTC CGGTTGGCTG GTGGAAATGA TGTTGGCATA
    TTTGTGGCTG GCATTCAGGA GGGCACCTCT GCAGAGCAGG AGGGCCTACA AGAAGGGGAC
    CAGATTCTGA AGGTGAACAC ACAAGATTTC AGAGGGCTGG TCCGGGAAGA TGCCGTCCTC
    TACCTGTTAG AAATCCCTAA AGGTGAAACC GTGACCATTT TGGCTCAGAG CCGAGCAGAC
    GTGTATAGAG ACATCCTGGC CTGTGGCAGG GGAGACTCGT TCTTCATAAG GAGCCACTTT
    GAATGTGAGA AGGAGACTCC GCAGAGCTTG GCCTTCACCA GGGGAGAGGT CTTCCGAGTG
    GTAGACACGC TGTACGATGG CAAACTGGGC CACTGGCTGG CTGTGAGGAT TGGAAATGAG
    CTGGAGAAGG GCTTAATCCC TAACAAAAGC AGAGCTGAGC AGATGGCCAG TGTCCAGAAT
    GCCCAGCGAG AGAATGCTGG GGACAGAGCA GACTTCTGGC GGATGCGTGG CCAGAGATCC
    GGGGTCAAGA AGAACATTCG CAAGAGCCGG GAAGACCTGG CAGCTGCTGT GTCGGTTAGC
    ACCAAGTTCC CCGCCTACGA GAAGGTTCTG CTTCGGGAAG CTGGCTTCAA GAGACCCGTG
    GTTCTGTTTG GCCCCATAGC AGATATAGCA ATGGAAAGGC TGACTACTGA GCTACCCGAC
    CTGTTTCAAA CTGCAAAAAC AGAACCCAAA GATGCGGGAT CTGAGAAATC CAGTGGAGTG
    GTTCGGTTGA ATACTGTGAG GCAAATTATT GAGCAGGACA AGCATGCCCT GCTCGACGTT
    ACCCCCAAAG CTGTGGACCT GCTCAATTAT ACTCAGTGGT TCCCAATCGT GATTTTCTTC
    AACCCGGATT CCAGACAAGG CATTAAAACC ATAAGGCAGA GGTTGAACCC AACATCCAAT
    AAAAGTTCTC GCAAGTTATT CGATCAAGCC AACAAGCTCA AAAAAACCTG TTCTCATCTT
    TTTACAGCTA CCATCAACGT GAATTCAGCC AATGATGGCT GGTTTGGCAG CCTGAAGGAC
    AGCATTCAGC AGCAGCAAAA CGAAGCAGTG TGGGTCTCTG AAGGAAAGAT GGAGGGGATG
    GATGATGACG CTGAAGACCG CATGTCCTAC TTAACCGCCA TGGGTGCGGA CTATCTGAGT
    TGTGACAGCC GTCTCATCAG TGACTTTGAA GATACGGACG GCGAGGGAGG CGCCTACACT
    GACAATGAGC TGGATGAGCC AGCTGAGGAG CCGCTGGTGT CTTCCATCAC CCGCTCCTCA
    GAGCCGGTGC AGCATGAGGA GAGCATAAGG AAGCCCAGCC CAGAGCCACG CGCTCAGATG
    AGGAGGGCAG CTAGCAGAGA CCAGCTTAGG GATGGTAGCC CGCCCCCAGC ATTCAAGCCA
    GAGCCGCCCA AGGTCAGAAA CCAAAACAGA GAGGACTCTT TCAACTACTC CAAGTCAAAC
    CTCTCTGCCA TGGCTGGCAG TGAAATCCCG GGGGGATCCA CCAAAGGGTG TCCTCCCCCT
    ATTGCGGTGA AACCTGCCTT TGGGCGATCC ATCCTGAAGC CTTCTACTCC AGTCCCCATG
    CCTGAGAGTG AGGAGGTTGG GGAGAGCACC GAGGAGCAGG AAGAGGCTCC CAAATCAGTC
    CTGGGCAGAG TGAAAATCTT CGAGAAGATG GACCACAAGG CGAAATTACA GAGGATGCAG
    GAGCTCCAAG AAGCACAGAA TGCGAGGATT GAAATAGCTC AGAAGCATCC TGACATCTAT
    GCGGTTCCAA TCAAAGCCCC CAAGCCAGAT GCTGGCCTGC CCCAGCACAT GAGTTCTAGA
    CCCCCAGAGC CACAGAAAGC TCCTTCTAGG CTTTACCAGG ACACCAGAGG AAGCTACGGC
    AGTGATCCCG AGGAAGAGGA GGAGTACCGC CAACAGTTGG CAGCACACTC GAAGCGTGGT
    TACTACAGCC AGCCCTCCCG GTACCGAGAC ACCGAATTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006231239.1
    CDS539..4120
    Translation

    Target ORF information:

    RefSeq Version XM_006231177.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006231177.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGGGCTG CTCAGGTCTT CCAGAGGATG TGGATCCAGG CAGTTAAAAA GTTGGAGAGA 
    TGGAAAGGCC ATGGCCCAGG CATGGAGGAG GTGATATGGG AACAGTACAC TGTGACCCTG
    CAGAAGGATT CCAAAAGAGG ATTTGGAATC GCAGTGTCAG GAGGCAGAGA CAACCCCCAC
    TTTGAAAATG GGGAGACGTC AATCGTCATT TCAGATGTAC TCCCAGGTGG ACCCGCTGAT
    GGCTTGCTTC AAGAAAATGA CAGGGTGGTC ATGGTCAATG GCACCCCCAT GGAGGATGTG
    CTCCATTCAT TTGCCGTTCA GCAGCTTAGG AAAAGTGGGA AGATCGCAGC CATTGTGGTC
    AAGAGGCCTC GGAAGGTTCA GGTGGCCCCG CTGCAGGGCA GCCCCCCTCT CAGTCATGAT
    GACCGAGGTT TTGAGATGAT GGATGAATTT GATGGCAGAA GTTTCCGCAG CGGCTACAGT
    GAGAGGAGCC GGCACAGCAG CCACGATATG CTCAGCCACA GCTGGGAGGA CAGCAGGGAG
    AGGGGGCGTC CCCATCAGCG CGCACAGAGC CGGGAGCGGG AGCGCAGCCG TGGCCGGAGC
    CTGGAACGTG GCCTGGACCA TGAAGACTAC GGGCGCAGCC ATGAGCGCAG CCGCGGCCGG
    AGCCTGGAGC GCGGTCTAGA CCGCGACTTT GTGTCCCGGG ACTACAGCCG TGGACGGAGC
    ATCGACAGGG ACTACGACCG AGACTATGAG CGCTCCTATC ACCAAGCCTA TGAGCCTGAC
    TACGAAGGCC GCTACAGCCC ATCTTATGAC CGCAGAGCCC ATCCAGAGAC CCGCTATGAA
    CGGAGCCGCA GCCGAGAACA CCTACGGTCC CGAAGCCCCA CCCCCGAGTC TAGGTCCCGG
    CAGGAACACA AGGGCCAGCA TGATCCCGAC AGGCCCATCG GGGTCCTTCT GACCAAAAGC
    AAAGCAAACG AAGAGTACGG GCTCCGGCTG GGAAGTCAGA TCTTCATTAA GGAAATGACC
    AGAACAGGAC TGGCAACAAA AGATGGCAAC CTTCATGAGG GGGACATAAT TCTCAAGATC
    AATGGAACTG TTACTGAGAA CATGTCTTTA ACCGATGCTC GGAAGTTAAT AGAAAAGTCT
    CGAGGAAAAC TGCAGCTGGT GGTGTTGAGA GACAGCAAGC AGACCCTCAT CAACATCCCA
    TCTCTGAATG ACAGCGACTC GGAAGTGGAG GATATCTCGG AAATCGAGTC CAACCGATCT
    TTTTCTCCAG AGGAGAGGCG CCAGCAGTAT TCTGATCAGG AGTATCATTC CTCCACTGAG
    AAGCTGAAGG AGAGGCCAAG CTCAAGAGAG GAGACCTCAG GCAGAATGTC CAGGATGGGT
    GCCACACCCA CGCCGTTCAA GTCCACGGGG GACATCACAG CTGCAGGTGT CACAGAGGCC
    AACAAGGAGC CCAGGTCCCA GGAGGAATCC CCAGTTCCTC AACCCAGAAC AGCATCAAGA
    GTCTTTCTTC GTCCTAGTCC CGAAGATGAA GCAATATATG GCCCTAACAC CAAAATGGTG
    AAGTTCAAGA AGGGAGACAG CGTGGGCCTC CGGTTGGCTG GTGGAAATGA TGTTGGCATA
    TTTGTGGCTG GCATTCAGGA GGGCACCTCT GCAGAGCAGG AGGGCCTACA AGAAGGGGAC
    CAGATTCTGA AGGTGAACAC ACAAGATTTC AGAGGGCTGG TCCGGGAAGA TGCCGTCCTC
    TACCTGTTAG AAATCCCTAA AGGTGAAACC GTGACCATTT TGGCTCAGAG CCGAGCAGAC
    GTGTATAGAG ACATCCTGGC CTGTGGCAGG GGAGACTCGT TCTTCATAAG GAGCCACTTT
    GAATGTGAGA AGGAGACTCC GCAGAGCTTG GCCTTCACCA GGGGAGAGGT CTTCCGAGTG
    GTAGACACGC TGTACGATGG CAAACTGGGC CACTGGCTGG CTGTGAGGAT TGGAAATGAG
    CTGGAGAAGG GCTTAATCCC TAACAAAAGC AGAGCTGAGC AGATGGCCAG TGTCCAGAAT
    GCCCAGCGAG AGAATGCTGG GGACAGAGCA GACTTCTGGC GGATGCGTGG CCAGAGATCC
    GGGGTCAAGA AGAACATTCG CAAGAGCCGG GAAGACCTGG CAGCTGCTGT GTCGGTTAGC
    ACCAAGTTCC CCGCCTACGA GAAGGTTCTG CTTCGGGAAG CTGGCTTCAA GAGACCCGTG
    GTTCTGTTTG GCCCCATAGC AGATATAGCA ATGGAAAGGC TGACTACTGA GCTACCCGAC
    CTGTTTCAAA CTGCAAAAAC AGAACCCAAA GATGCGGGAT CTGAGAAATC CAGTGGAGTG
    GTTCGGTTGA ATACTGTGAG GCAAATTATT GAGCAGGACA AGCATGCCCT GCTCGACGTT
    ACCCCCAAAG CTGTGGACCT GCTCAATTAT ACTCAGTGGT TCCCAATCGT GATTTTCTTC
    AACCCGGATT CCAGACAAGG CATTAAAACC ATAAGGCAGA GGTTGAACCC AACATCCAAT
    AAAAGTTCTC GCAAGTTATT CGATCAAGCC AACAAGCTCA AAAAAACCTG TTCTCATCTT
    TTTACAGCTA CCATCAACGT GAATTCAGCC AATGATGGCT GGTTTGGCAG CCTGAAGGAC
    AGCATTCAGC AGCAGCAAAA CGAAGCAGTG TGGGTCTCTG AAGGAAAGAT GGAGGGGATG
    GATGATGACG CTGAAGACCG CATGTCCTAC TTAACCGCCA TGGGTGCGGA CTATCTGAGT
    TGTGACAGCC GTCTCATCAG TGACTTTGAA GATACGGACG GCGAGGGAGG CGCCTACACT
    GACAATGAGC TGGATGAGCC AGCTGAGGAG CCGCTGGTGT CTTCCATCAC CCGCTCCTCA
    GAGCCGGTGC AGCATGAGGA GAGCATAAGG AAGCCCAGCC CAGAGCCACG CGCTCAGATG
    AGGAGGGCAG CTAGCAGAGA CCAGCTTAGG GATGGTAGCC CGCCCCCAGC ATTCAAGCCA
    GAGCCGCCCA AGGTCAGAAA CCAAAACAGA GAGGACTCTT TCAACTACTC CAAGTCAAAC
    CTCTCTGCCA TGGCTGGCAG TGAAATCCCG GGGGGATCCA CCAAAGGGTG TCCTCCCCCT
    ATTGCGGTGA AACCTGCCTT TGGGCGATCC ATCCTGAAGC CTTCTACTCC AGTCCCCATG
    CCTGAGAGTG AGGAGGTTGG GGAGAGCACC GAGGAGCAGG AAGAGGCTCC CAAATCAGTC
    CTGGGCAGAG TGAAAATCTT CGAGAAGATG GACCACAAGG CGAAATTACA GAGGATGCAG
    GAGCTCCAAG AAGCACAGAA TGCGAGGATT GAAATAGCTC AGAAGCATCC TGACATCTAT
    GCGGTTCCAA TCAAAGCCCC CAAGCCAGAT GCTGGCCTGC CCCAGCACAT GAGTTCTAGA
    CCCCCAGAGC CACAGAAAGC TCCTTCTAGG CTTTACCAGG ACACCAGAGG AAGCTACGGC
    AGTGATCCCG AGGAAGAGGA GGAGTACCGC CAACAGTTGG CAGCACACTC GAAGCGTGGT
    TACTACAGCC AGCCCTCCCG GTACCGAGAC ACCGAATTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa23974
    Clone ID Related Accession (Same CDS sequence) XM_006231178.3
    Accession Version XM_006231178.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3570bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product tight junction protein ZO-2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231178.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCGGTGC GGGGAGACCG CGGGTTCCCA CTGCGGCGGG AGCTGTCGGG CTGGCAGCGC 
    GGCCCAGGCA TGGAGGAGGT GATATGGGAA CAGTACACTG TGACCCTGCA GAAGGATTCC
    AAAAGAGGAT TTGGAATCGC AGTGTCAGGA GGCAGAGACA ACCCCCACTT TGAAAATGGG
    GAGACGTCAA TCGTCATTTC AGATGTACTC CCAGGTGGAC CCGCTGATGG CTTGCTTCAA
    GAAAATGACA GGGTGGTCAT GGTCAATGGC ACCCCCATGG AGGATGTGCT CCATTCATTT
    GCCGTTCAGC AGCTTAGGAA AAGTGGGAAG ATCGCAGCCA TTGTGGTCAA GAGGCCTCGG
    AAGGTTCAGG TGGCCCCGCT GCAGGGCAGC CCCCCTCTCA GTCATGATGA CCGAGGTTTT
    GAGATGATGG ATGAATTTGA TGGCAGAAGT TTCCGCAGCG GCTACAGTGA GAGGAGCCGG
    CACAGCAGCC ACGATATGCT CAGCCACAGC TGGGAGGACA GCAGGGAGAG GGGGCGTCCC
    CATCAGCGCG CACAGAGCCG GGAGCGGGAG CGCAGCCGTG GCCGGAGCCT GGAACGTGGC
    CTGGACCATG AAGACTACGG GCGCAGCCAT GAGCGCAGCC GCGGCCGGAG CCTGGAGCGC
    GGTCTAGACC GCGACTTTGT GTCCCGGGAC TACAGCCGTG GACGGAGCAT CGACAGGGAC
    TACGACCGAG ACTATGAGCG CTCCTATCAC CAAGCCTATG AGCCTGACTA CGAAGGCCGC
    TACAGCCCAT CTTATGACCG CAGAGCCCAT CCAGAGACCC GCTATGAACG GAGCCGCAGC
    CGAGAACACC TACGGTCCCG AAGCCCCACC CCCGAGTCTA GGTCCCGGCA GGAACACAAG
    GGCCAGCATG ATCCCGACAG GCCCATCGGG GTCCTTCTGA CCAAAAGCAA AGCAAACGAA
    GAGTACGGGC TCCGGCTGGG AAGTCAGATC TTCATTAAGG AAATGACCAG AACAGGACTG
    GCAACAAAAG ATGGCAACCT TCATGAGGGG GACATAATTC TCAAGATCAA TGGAACTGTT
    ACTGAGAACA TGTCTTTAAC CGATGCTCGG AAGTTAATAG AAAAGTCTCG AGGAAAACTG
    CAGCTGGTGG TGTTGAGAGA CAGCAAGCAG ACCCTCATCA ACATCCCATC TCTGAATGAC
    AGCGACTCGG AAGTGGAGGA TATCTCGGAA ATCGAGTCCA ACCGATCTTT TTCTCCAGAG
    GAGAGGCGCC AGCAGTATTC TGATCAGGAG TATCATTCCT CCACTGAGAA GCTGAAGGAG
    AGGCCAAGCT CAAGAGAGGA GACCTCAGGC AGAATGTCCA GGATGGGTGC CACACCCACG
    CCGTTCAAGT CCACGGGGGA CATCACAGCT GCAGGTGTCA CAGAGGCCAA CAAGGAGCCC
    AGGTCCCAGG AGGAATCCCC AGTTCCTCAA CCCAGAACAG CATCAAGAGT CTTTCTTCGT
    CCTAGTCCCG AAGATGAAGC AATATATGGC CCTAACACCA AAATGGTGAA GTTCAAGAAG
    GGAGACAGCG TGGGCCTCCG GTTGGCTGGT GGAAATGATG TTGGCATATT TGTGGCTGGC
    ATTCAGGAGG GCACCTCTGC AGAGCAGGAG GGCCTACAAG AAGGGGACCA GATTCTGAAG
    GTGAACACAC AAGATTTCAG AGGGCTGGTC CGGGAAGATG CCGTCCTCTA CCTGTTAGAA
    ATCCCTAAAG GTGAAACCGT GACCATTTTG GCTCAGAGCC GAGCAGACGT GTATAGAGAC
    ATCCTGGCCT GTGGCAGGGG AGACTCGTTC TTCATAAGGA GCCACTTTGA ATGTGAGAAG
    GAGACTCCGC AGAGCTTGGC CTTCACCAGG GGAGAGGTCT TCCGAGTGGT AGACACGCTG
    TACGATGGCA AACTGGGCCA CTGGCTGGCT GTGAGGATTG GAAATGAGCT GGAGAAGGGC
    TTAATCCCTA ACAAAAGCAG AGCTGAGCAG ATGGCCAGTG TCCAGAATGC CCAGCGAGAG
    AATGCTGGGG ACAGAGCAGA CTTCTGGCGG ATGCGTGGCC AGAGATCCGG GGTCAAGAAG
    AACATTCGCA AGAGCCGGGA AGACCTGGCA GCTGCTGTGT CGGTTAGCAC CAAGTTCCCC
    GCCTACGAGA AGGTTCTGCT TCGGGAAGCT GGCTTCAAGA GACCCGTGGT TCTGTTTGGC
    CCCATAGCAG ATATAGCAAT GGAAAGGCTG ACTACTGAGC TACCCGACCT GTTTCAAACT
    GCAAAAACAG AACCCAAAGA TGCGGGATCT GAGAAATCCA GTGGAGTGGT TCGGTTGAAT
    ACTGTGAGGC AAATTATTGA GCAGGACAAG CATGCCCTGC TCGACGTTAC CCCCAAAGCT
    GTGGACCTGC TCAATTATAC TCAGTGGTTC CCAATCGTGA TTTTCTTCAA CCCGGATTCC
    AGACAAGGCA TTAAAACCAT AAGGCAGAGG TTGAACCCAA CATCCAATAA AAGTTCTCGC
    AAGTTATTCG ATCAAGCCAA CAAGCTCAAA AAAACCTGTT CTCATCTTTT TACAGCTACC
    ATCAACGTGA ATTCAGCCAA TGATGGCTGG TTTGGCAGCC TGAAGGACAG CATTCAGCAG
    CAGCAAAACG AAGCAGTGTG GGTCTCTGAA GGAAAGATGG AGGGGATGGA TGATGACGCT
    GAAGACCGCA TGTCCTACTT AACCGCCATG GGTGCGGACT ATCTGAGTTG TGACAGCCGT
    CTCATCAGTG ACTTTGAAGA TACGGACGGC GAGGGAGGCG CCTACACTGA CAATGAGCTG
    GATGAGCCAG CTGAGGAGCC GCTGGTGTCT TCCATCACCC GCTCCTCAGA GCCGGTGCAG
    CATGAGGAGA GCATAAGGAA GCCCAGCCCA GAGCCACGCG CTCAGATGAG GAGGGCAGCT
    AGCAGAGACC AGCTTAGGGA TGGTAGCCCG CCCCCAGCAT TCAAGCCAGA GCCGCCCAAG
    GTCAGAAACC AAAACAGAGA GGACTCTTTC AACTACTCCA AGTCAAACCT CTCTGCCATG
    GCTGGCAGTG AAATCCCGGG GGGATCCACC AAAGGGTGTC CTCCCCCTAT TGCGGTGAAA
    CCTGCCTTTG GGCGATCCAT CCTGAAGCCT TCTACTCCAG TCCCCATGCC TGAGAGTGAG
    GAGGTTGGGG AGAGCACCGA GGAGCAGGAA GAGGCTCCCA AATCAGTCCT GGGCAGAGTG
    AAAATCTTCG AGAAGATGGA CCACAAGGCG AAATTACAGA GGATGCAGGA GCTCCAAGAA
    GCACAGAATG CGAGGATTGA AATAGCTCAG AAGCATCCTG ACATCTATGC GGTTCCAATC
    AAAGCCCCCA AGCCAGATGC TGGCCTGCCC CAGCACATGA GTTCTAGACC CCCAGAGCCA
    CAGAAAGCTC CTTCTAGGCT TTACCAGGAC ACCAGAGGAA GCTACGGCAG TGATCCCGAG
    GAAGAGGAGG AGTACCGCCA ACAGTTGGCA GCACACTCGA AGCGTGGTTA CTACAGCCAG
    CCCTCCCGGT ACCGAGACAC CGAATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006231240.1
    CDS2363..5932
    Translation

    Target ORF information:

    RefSeq Version XM_006231178.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006231178.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCGGTGC GGGGAGACCG CGGGTTCCCA CTGCGGCGGG AGCTGTCGGG CTGGCAGCGC 
    GGCCCAGGCA TGGAGGAGGT GATATGGGAA CAGTACACTG TGACCCTGCA GAAGGATTCC
    AAAAGAGGAT TTGGAATCGC AGTGTCAGGA GGCAGAGACA ACCCCCACTT TGAAAATGGG
    GAGACGTCAA TCGTCATTTC AGATGTACTC CCAGGTGGAC CCGCTGATGG CTTGCTTCAA
    GAAAATGACA GGGTGGTCAT GGTCAATGGC ACCCCCATGG AGGATGTGCT CCATTCATTT
    GCCGTTCAGC AGCTTAGGAA AAGTGGGAAG ATCGCAGCCA TTGTGGTCAA GAGGCCTCGG
    AAGGTTCAGG TGGCCCCGCT GCAGGGCAGC CCCCCTCTCA GTCATGATGA CCGAGGTTTT
    GAGATGATGG ATGAATTTGA TGGCAGAAGT TTCCGCAGCG GCTACAGTGA GAGGAGCCGG
    CACAGCAGCC ACGATATGCT CAGCCACAGC TGGGAGGACA GCAGGGAGAG GGGGCGTCCC
    CATCAGCGCG CACAGAGCCG GGAGCGGGAG CGCAGCCGTG GCCGGAGCCT GGAACGTGGC
    CTGGACCATG AAGACTACGG GCGCAGCCAT GAGCGCAGCC GCGGCCGGAG CCTGGAGCGC
    GGTCTAGACC GCGACTTTGT GTCCCGGGAC TACAGCCGTG GACGGAGCAT CGACAGGGAC
    TACGACCGAG ACTATGAGCG CTCCTATCAC CAAGCCTATG AGCCTGACTA CGAAGGCCGC
    TACAGCCCAT CTTATGACCG CAGAGCCCAT CCAGAGACCC GCTATGAACG GAGCCGCAGC
    CGAGAACACC TACGGTCCCG AAGCCCCACC CCCGAGTCTA GGTCCCGGCA GGAACACAAG
    GGCCAGCATG ATCCCGACAG GCCCATCGGG GTCCTTCTGA CCAAAAGCAA AGCAAACGAA
    GAGTACGGGC TCCGGCTGGG AAGTCAGATC TTCATTAAGG AAATGACCAG AACAGGACTG
    GCAACAAAAG ATGGCAACCT TCATGAGGGG GACATAATTC TCAAGATCAA TGGAACTGTT
    ACTGAGAACA TGTCTTTAAC CGATGCTCGG AAGTTAATAG AAAAGTCTCG AGGAAAACTG
    CAGCTGGTGG TGTTGAGAGA CAGCAAGCAG ACCCTCATCA ACATCCCATC TCTGAATGAC
    AGCGACTCGG AAGTGGAGGA TATCTCGGAA ATCGAGTCCA ACCGATCTTT TTCTCCAGAG
    GAGAGGCGCC AGCAGTATTC TGATCAGGAG TATCATTCCT CCACTGAGAA GCTGAAGGAG
    AGGCCAAGCT CAAGAGAGGA GACCTCAGGC AGAATGTCCA GGATGGGTGC CACACCCACG
    CCGTTCAAGT CCACGGGGGA CATCACAGCT GCAGGTGTCA CAGAGGCCAA CAAGGAGCCC
    AGGTCCCAGG AGGAATCCCC AGTTCCTCAA CCCAGAACAG CATCAAGAGT CTTTCTTCGT
    CCTAGTCCCG AAGATGAAGC AATATATGGC CCTAACACCA AAATGGTGAA GTTCAAGAAG
    GGAGACAGCG TGGGCCTCCG GTTGGCTGGT GGAAATGATG TTGGCATATT TGTGGCTGGC
    ATTCAGGAGG GCACCTCTGC AGAGCAGGAG GGCCTACAAG AAGGGGACCA GATTCTGAAG
    GTGAACACAC AAGATTTCAG AGGGCTGGTC CGGGAAGATG CCGTCCTCTA CCTGTTAGAA
    ATCCCTAAAG GTGAAACCGT GACCATTTTG GCTCAGAGCC GAGCAGACGT GTATAGAGAC
    ATCCTGGCCT GTGGCAGGGG AGACTCGTTC TTCATAAGGA GCCACTTTGA ATGTGAGAAG
    GAGACTCCGC AGAGCTTGGC CTTCACCAGG GGAGAGGTCT TCCGAGTGGT AGACACGCTG
    TACGATGGCA AACTGGGCCA CTGGCTGGCT GTGAGGATTG GAAATGAGCT GGAGAAGGGC
    TTAATCCCTA ACAAAAGCAG AGCTGAGCAG ATGGCCAGTG TCCAGAATGC CCAGCGAGAG
    AATGCTGGGG ACAGAGCAGA CTTCTGGCGG ATGCGTGGCC AGAGATCCGG GGTCAAGAAG
    AACATTCGCA AGAGCCGGGA AGACCTGGCA GCTGCTGTGT CGGTTAGCAC CAAGTTCCCC
    GCCTACGAGA AGGTTCTGCT TCGGGAAGCT GGCTTCAAGA GACCCGTGGT TCTGTTTGGC
    CCCATAGCAG ATATAGCAAT GGAAAGGCTG ACTACTGAGC TACCCGACCT GTTTCAAACT
    GCAAAAACAG AACCCAAAGA TGCGGGATCT GAGAAATCCA GTGGAGTGGT TCGGTTGAAT
    ACTGTGAGGC AAATTATTGA GCAGGACAAG CATGCCCTGC TCGACGTTAC CCCCAAAGCT
    GTGGACCTGC TCAATTATAC TCAGTGGTTC CCAATCGTGA TTTTCTTCAA CCCGGATTCC
    AGACAAGGCA TTAAAACCAT AAGGCAGAGG TTGAACCCAA CATCCAATAA AAGTTCTCGC
    AAGTTATTCG ATCAAGCCAA CAAGCTCAAA AAAACCTGTT CTCATCTTTT TACAGCTACC
    ATCAACGTGA ATTCAGCCAA TGATGGCTGG TTTGGCAGCC TGAAGGACAG CATTCAGCAG
    CAGCAAAACG AAGCAGTGTG GGTCTCTGAA GGAAAGATGG AGGGGATGGA TGATGACGCT
    GAAGACCGCA TGTCCTACTT AACCGCCATG GGTGCGGACT ATCTGAGTTG TGACAGCCGT
    CTCATCAGTG ACTTTGAAGA TACGGACGGC GAGGGAGGCG CCTACACTGA CAATGAGCTG
    GATGAGCCAG CTGAGGAGCC GCTGGTGTCT TCCATCACCC GCTCCTCAGA GCCGGTGCAG
    CATGAGGAGA GCATAAGGAA GCCCAGCCCA GAGCCACGCG CTCAGATGAG GAGGGCAGCT
    AGCAGAGACC AGCTTAGGGA TGGTAGCCCG CCCCCAGCAT TCAAGCCAGA GCCGCCCAAG
    GTCAGAAACC AAAACAGAGA GGACTCTTTC AACTACTCCA AGTCAAACCT CTCTGCCATG
    GCTGGCAGTG AAATCCCGGG GGGATCCACC AAAGGGTGTC CTCCCCCTAT TGCGGTGAAA
    CCTGCCTTTG GGCGATCCAT CCTGAAGCCT TCTACTCCAG TCCCCATGCC TGAGAGTGAG
    GAGGTTGGGG AGAGCACCGA GGAGCAGGAA GAGGCTCCCA AATCAGTCCT GGGCAGAGTG
    AAAATCTTCG AGAAGATGGA CCACAAGGCG AAATTACAGA GGATGCAGGA GCTCCAAGAA
    GCACAGAATG CGAGGATTGA AATAGCTCAG AAGCATCCTG ACATCTATGC GGTTCCAATC
    AAAGCCCCCA AGCCAGATGC TGGCCTGCCC CAGCACATGA GTTCTAGACC CCCAGAGCCA
    CAGAAAGCTC CTTCTAGGCT TTACCAGGAC ACCAGAGGAA GCTACGGCAG TGATCCCGAG
    GAAGAGGAGG AGTACCGCCA ACAGTTGGCA GCACACTCGA AGCGTGGTTA CTACAGCCAG
    CCCTCCCGGT ACCGAGACAC CGAATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa23977
    Clone ID Related Accession (Same CDS sequence) XM_006231181.3 , XM_008760302.2
    Accession Version XM_006231181.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3501bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product tight junction protein ZO-2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231181.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGGAGG TGATATGGGA ACAGTACACT GTGACCCTGC AGAAGGATTC CAAAAGAGGA 
    TTTGGAATCG CAGTGTCAGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACGTCA
    ATCGTCATTT CAGATGTACT CCCAGGTGGA CCCGCTGATG GCTTGCTTCA AGAAAATGAC
    AGGGTGGTCA TGGTCAATGG CACCCCCATG GAGGATGTGC TCCATTCATT TGCCGTTCAG
    CAGCTTAGGA AAAGTGGGAA GATCGCAGCC ATTGTGGTCA AGAGGCCTCG GAAGGTTCAG
    GTGGCCCCGC TGCAGGGCAG CCCCCCTCTC AGTCATGATG ACCGAGGTTT TGAGATGATG
    GATGAATTTG ATGGCAGAAG TTTCCGCAGC GGCTACAGTG AGAGGAGCCG GCACAGCAGC
    CACGATATGC TCAGCCACAG CTGGGAGGAC AGCAGGGAGA GGGGGCGTCC CCATCAGCGC
    GCACAGAGCC GGGAGCGGGA GCGCAGCCGT GGCCGGAGCC TGGAACGTGG CCTGGACCAT
    GAAGACTACG GGCGCAGCCA TGAGCGCAGC CGCGGCCGGA GCCTGGAGCG CGGTCTAGAC
    CGCGACTTTG TGTCCCGGGA CTACAGCCGT GGACGGAGCA TCGACAGGGA CTACGACCGA
    GACTATGAGC GCTCCTATCA CCAAGCCTAT GAGCCTGACT ACGAAGGCCG CTACAGCCCA
    TCTTATGACC GCAGAGCCCA TCCAGAGACC CGCTATGAAC GGAGCCGCAG CCGAGAACAC
    CTACGGTCCC GAAGCCCCAC CCCCGAGTCT AGGTCCCGGC AGGAACACAA GGGCCAGCAT
    GATCCCGACA GGCCCATCGG GGTCCTTCTG ACCAAAAGCA AAGCAAACGA AGAGTACGGG
    CTCCGGCTGG GAAGTCAGAT CTTCATTAAG GAAATGACCA GAACAGGACT GGCAACAAAA
    GATGGCAACC TTCATGAGGG GGACATAATT CTCAAGATCA ATGGAACTGT TACTGAGAAC
    ATGTCTTTAA CCGATGCTCG GAAGTTAATA GAAAAGTCTC GAGGAAAACT GCAGCTGGTG
    GTGTTGAGAG ACAGCAAGCA GACCCTCATC AACATCCCAT CTCTGAATGA CAGCGACTCG
    GAAGTGGAGG ATATCTCGGA AATCGAGTCC AACCGATCTT TTTCTCCAGA GGAGAGGCGC
    CAGCAGTATT CTGATCAGGA GTATCATTCC TCCACTGAGA AGCTGAAGGA GAGGCCAAGC
    TCAAGAGAGG AGACCTCAGG CAGAATGTCC AGGATGGGTG CCACACCCAC GCCGTTCAAG
    TCCACGGGGG ACATCACAGC TGCAGGTGTC ACAGAGGCCA ACAAGGAGCC CAGGTCCCAG
    GAGGAATCCC CAGTTCCTCA ACCCAGAACA GCATCAAGAG TCTTTCTTCG TCCTAGTCCC
    GAAGATGAAG CAATATATGG CCCTAACACC AAAATGGTGA AGTTCAAGAA GGGAGACAGC
    GTGGGCCTCC GGTTGGCTGG TGGAAATGAT GTTGGCATAT TTGTGGCTGG CATTCAGGAG
    GGCACCTCTG CAGAGCAGGA GGGCCTACAA GAAGGGGACC AGATTCTGAA GGTGAACACA
    CAAGATTTCA GAGGGCTGGT CCGGGAAGAT GCCGTCCTCT ACCTGTTAGA AATCCCTAAA
    GGTGAAACCG TGACCATTTT GGCTCAGAGC CGAGCAGACG TGTATAGAGA CATCCTGGCC
    TGTGGCAGGG GAGACTCGTT CTTCATAAGG AGCCACTTTG AATGTGAGAA GGAGACTCCG
    CAGAGCTTGG CCTTCACCAG GGGAGAGGTC TTCCGAGTGG TAGACACGCT GTACGATGGC
    AAACTGGGCC ACTGGCTGGC TGTGAGGATT GGAAATGAGC TGGAGAAGGG CTTAATCCCT
    AACAAAAGCA GAGCTGAGCA GATGGCCAGT GTCCAGAATG CCCAGCGAGA GAATGCTGGG
    GACAGAGCAG ACTTCTGGCG GATGCGTGGC CAGAGATCCG GGGTCAAGAA GAACATTCGC
    AAGAGCCGGG AAGACCTGGC AGCTGCTGTG TCGGTTAGCA CCAAGTTCCC CGCCTACGAG
    AAGGTTCTGC TTCGGGAAGC TGGCTTCAAG AGACCCGTGG TTCTGTTTGG CCCCATAGCA
    GATATAGCAA TGGAAAGGCT GACTACTGAG CTACCCGACC TGTTTCAAAC TGCAAAAACA
    GAACCCAAAG ATGCGGGATC TGAGAAATCC AGTGGAGTGG TTCGGTTGAA TACTGTGAGG
    CAAATTATTG AGCAGGACAA GCATGCCCTG CTCGACGTTA CCCCCAAAGC TGTGGACCTG
    CTCAATTATA CTCAGTGGTT CCCAATCGTG ATTTTCTTCA ACCCGGATTC CAGACAAGGC
    ATTAAAACCA TAAGGCAGAG GTTGAACCCA ACATCCAATA AAAGTTCTCG CAAGTTATTC
    GATCAAGCCA ACAAGCTCAA AAAAACCTGT TCTCATCTTT TTACAGCTAC CATCAACGTG
    AATTCAGCCA ATGATGGCTG GTTTGGCAGC CTGAAGGACA GCATTCAGCA GCAGCAAAAC
    GAAGCAGTGT GGGTCTCTGA AGGAAAGATG GAGGGGATGG ATGATGACGC TGAAGACCGC
    ATGTCCTACT TAACCGCCAT GGGTGCGGAC TATCTGAGTT GTGACAGCCG TCTCATCAGT
    GACTTTGAAG ATACGGACGG CGAGGGAGGC GCCTACACTG ACAATGAGCT GGATGAGCCA
    GCTGAGGAGC CGCTGGTGTC TTCCATCACC CGCTCCTCAG AGCCGGTGCA GCATGAGGAG
    AGCATAAGGA AGCCCAGCCC AGAGCCACGC GCTCAGATGA GGAGGGCAGC TAGCAGAGAC
    CAGCTTAGGG ATGGTAGCCC GCCCCCAGCA TTCAAGCCAG AGCCGCCCAA GGTCAGAAAC
    CAAAACAGAG AGGACTCTTT CAACTACTCC AAGTCAAACC TCTCTGCCAT GGCTGGCAGT
    GAAATCCCGG GGGGATCCAC CAAAGGGTGT CCTCCCCCTA TTGCGGTGAA ACCTGCCTTT
    GGGCGATCCA TCCTGAAGCC TTCTACTCCA GTCCCCATGC CTGAGAGTGA GGAGGTTGGG
    GAGAGCACCG AGGAGCAGGA AGAGGCTCCC AAATCAGTCC TGGGCAGAGT GAAAATCTTC
    GAGAAGATGG ACCACAAGGC GAAATTACAG AGGATGCAGG AGCTCCAAGA AGCACAGAAT
    GCGAGGATTG AAATAGCTCA GAAGCATCCT GACATCTATG CGGTTCCAAT CAAAGCCCCC
    AAGCCAGATG CTGGCCTGCC CCAGCACATG AGTTCTAGAC CCCCAGAGCC ACAGAAAGCT
    CCTTCTAGGC TTTACCAGGA CACCAGAGGA AGCTACGGCA GTGATCCCGA GGAAGAGGAG
    GAGTACCGCC AACAGTTGGC AGCACACTCG AAGCGTGGTT ACTACAGCCA GCCCTCCCGG
    TACCGAGACA CCGAATTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006231243.1
    CDS411..3911
    Translation

    Target ORF information:

    RefSeq Version XM_006231181.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006231181.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGGAGG TGATATGGGA ACAGTACACT GTGACCCTGC AGAAGGATTC CAAAAGAGGA 
    TTTGGAATCG CAGTGTCAGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACGTCA
    ATCGTCATTT CAGATGTACT CCCAGGTGGA CCCGCTGATG GCTTGCTTCA AGAAAATGAC
    AGGGTGGTCA TGGTCAATGG CACCCCCATG GAGGATGTGC TCCATTCATT TGCCGTTCAG
    CAGCTTAGGA AAAGTGGGAA GATCGCAGCC ATTGTGGTCA AGAGGCCTCG GAAGGTTCAG
    GTGGCCCCGC TGCAGGGCAG CCCCCCTCTC AGTCATGATG ACCGAGGTTT TGAGATGATG
    GATGAATTTG ATGGCAGAAG TTTCCGCAGC GGCTACAGTG AGAGGAGCCG GCACAGCAGC
    CACGATATGC TCAGCCACAG CTGGGAGGAC AGCAGGGAGA GGGGGCGTCC CCATCAGCGC
    GCACAGAGCC GGGAGCGGGA GCGCAGCCGT GGCCGGAGCC TGGAACGTGG CCTGGACCAT
    GAAGACTACG GGCGCAGCCA TGAGCGCAGC CGCGGCCGGA GCCTGGAGCG CGGTCTAGAC
    CGCGACTTTG TGTCCCGGGA CTACAGCCGT GGACGGAGCA TCGACAGGGA CTACGACCGA
    GACTATGAGC GCTCCTATCA CCAAGCCTAT GAGCCTGACT ACGAAGGCCG CTACAGCCCA
    TCTTATGACC GCAGAGCCCA TCCAGAGACC CGCTATGAAC GGAGCCGCAG CCGAGAACAC
    CTACGGTCCC GAAGCCCCAC CCCCGAGTCT AGGTCCCGGC AGGAACACAA GGGCCAGCAT
    GATCCCGACA GGCCCATCGG GGTCCTTCTG ACCAAAAGCA AAGCAAACGA AGAGTACGGG
    CTCCGGCTGG GAAGTCAGAT CTTCATTAAG GAAATGACCA GAACAGGACT GGCAACAAAA
    GATGGCAACC TTCATGAGGG GGACATAATT CTCAAGATCA ATGGAACTGT TACTGAGAAC
    ATGTCTTTAA CCGATGCTCG GAAGTTAATA GAAAAGTCTC GAGGAAAACT GCAGCTGGTG
    GTGTTGAGAG ACAGCAAGCA GACCCTCATC AACATCCCAT CTCTGAATGA CAGCGACTCG
    GAAGTGGAGG ATATCTCGGA AATCGAGTCC AACCGATCTT TTTCTCCAGA GGAGAGGCGC
    CAGCAGTATT CTGATCAGGA GTATCATTCC TCCACTGAGA AGCTGAAGGA GAGGCCAAGC
    TCAAGAGAGG AGACCTCAGG CAGAATGTCC AGGATGGGTG CCACACCCAC GCCGTTCAAG
    TCCACGGGGG ACATCACAGC TGCAGGTGTC ACAGAGGCCA ACAAGGAGCC CAGGTCCCAG
    GAGGAATCCC CAGTTCCTCA ACCCAGAACA GCATCAAGAG TCTTTCTTCG TCCTAGTCCC
    GAAGATGAAG CAATATATGG CCCTAACACC AAAATGGTGA AGTTCAAGAA GGGAGACAGC
    GTGGGCCTCC GGTTGGCTGG TGGAAATGAT GTTGGCATAT TTGTGGCTGG CATTCAGGAG
    GGCACCTCTG CAGAGCAGGA GGGCCTACAA GAAGGGGACC AGATTCTGAA GGTGAACACA
    CAAGATTTCA GAGGGCTGGT CCGGGAAGAT GCCGTCCTCT ACCTGTTAGA AATCCCTAAA
    GGTGAAACCG TGACCATTTT GGCTCAGAGC CGAGCAGACG TGTATAGAGA CATCCTGGCC
    TGTGGCAGGG GAGACTCGTT CTTCATAAGG AGCCACTTTG AATGTGAGAA GGAGACTCCG
    CAGAGCTTGG CCTTCACCAG GGGAGAGGTC TTCCGAGTGG TAGACACGCT GTACGATGGC
    AAACTGGGCC ACTGGCTGGC TGTGAGGATT GGAAATGAGC TGGAGAAGGG CTTAATCCCT
    AACAAAAGCA GAGCTGAGCA GATGGCCAGT GTCCAGAATG CCCAGCGAGA GAATGCTGGG
    GACAGAGCAG ACTTCTGGCG GATGCGTGGC CAGAGATCCG GGGTCAAGAA GAACATTCGC
    AAGAGCCGGG AAGACCTGGC AGCTGCTGTG TCGGTTAGCA CCAAGTTCCC CGCCTACGAG
    AAGGTTCTGC TTCGGGAAGC TGGCTTCAAG AGACCCGTGG TTCTGTTTGG CCCCATAGCA
    GATATAGCAA TGGAAAGGCT GACTACTGAG CTACCCGACC TGTTTCAAAC TGCAAAAACA
    GAACCCAAAG ATGCGGGATC TGAGAAATCC AGTGGAGTGG TTCGGTTGAA TACTGTGAGG
    CAAATTATTG AGCAGGACAA GCATGCCCTG CTCGACGTTA CCCCCAAAGC TGTGGACCTG
    CTCAATTATA CTCAGTGGTT CCCAATCGTG ATTTTCTTCA ACCCGGATTC CAGACAAGGC
    ATTAAAACCA TAAGGCAGAG GTTGAACCCA ACATCCAATA AAAGTTCTCG CAAGTTATTC
    GATCAAGCCA ACAAGCTCAA AAAAACCTGT TCTCATCTTT TTACAGCTAC CATCAACGTG
    AATTCAGCCA ATGATGGCTG GTTTGGCAGC CTGAAGGACA GCATTCAGCA GCAGCAAAAC
    GAAGCAGTGT GGGTCTCTGA AGGAAAGATG GAGGGGATGG ATGATGACGC TGAAGACCGC
    ATGTCCTACT TAACCGCCAT GGGTGCGGAC TATCTGAGTT GTGACAGCCG TCTCATCAGT
    GACTTTGAAG ATACGGACGG CGAGGGAGGC GCCTACACTG ACAATGAGCT GGATGAGCCA
    GCTGAGGAGC CGCTGGTGTC TTCCATCACC CGCTCCTCAG AGCCGGTGCA GCATGAGGAG
    AGCATAAGGA AGCCCAGCCC AGAGCCACGC GCTCAGATGA GGAGGGCAGC TAGCAGAGAC
    CAGCTTAGGG ATGGTAGCCC GCCCCCAGCA TTCAAGCCAG AGCCGCCCAA GGTCAGAAAC
    CAAAACAGAG AGGACTCTTT CAACTACTCC AAGTCAAACC TCTCTGCCAT GGCTGGCAGT
    GAAATCCCGG GGGGATCCAC CAAAGGGTGT CCTCCCCCTA TTGCGGTGAA ACCTGCCTTT
    GGGCGATCCA TCCTGAAGCC TTCTACTCCA GTCCCCATGC CTGAGAGTGA GGAGGTTGGG
    GAGAGCACCG AGGAGCAGGA AGAGGCTCCC AAATCAGTCC TGGGCAGAGT GAAAATCTTC
    GAGAAGATGG ACCACAAGGC GAAATTACAG AGGATGCAGG AGCTCCAAGA AGCACAGAAT
    GCGAGGATTG AAATAGCTCA GAAGCATCCT GACATCTATG CGGTTCCAAT CAAAGCCCCC
    AAGCCAGATG CTGGCCTGCC CCAGCACATG AGTTCTAGAC CCCCAGAGCC ACAGAAAGCT
    CCTTCTAGGC TTTACCAGGA CACCAGAGGA AGCTACGGCA GTGATCCCGA GGAAGAGGAG
    GAGTACCGCC AACAGTTGGC AGCACACTCG AAGCGTGGTT ACTACAGCCA GCCCTCCCGG
    TACCGAGACA CCGAATTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa23977
    Clone ID Related Accession (Same CDS sequence) XM_006231181.3 , XM_008760302.2
    Accession Version XM_008760302.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3501bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product tight junction protein ZO-2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760302.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGGAGG TGATATGGGA ACAGTACACT GTGACCCTGC AGAAGGATTC CAAAAGAGGA 
    TTTGGAATCG CAGTGTCAGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACGTCA
    ATCGTCATTT CAGATGTACT CCCAGGTGGA CCCGCTGATG GCTTGCTTCA AGAAAATGAC
    AGGGTGGTCA TGGTCAATGG CACCCCCATG GAGGATGTGC TCCATTCATT TGCCGTTCAG
    CAGCTTAGGA AAAGTGGGAA GATCGCAGCC ATTGTGGTCA AGAGGCCTCG GAAGGTTCAG
    GTGGCCCCGC TGCAGGGCAG CCCCCCTCTC AGTCATGATG ACCGAGGTTT TGAGATGATG
    GATGAATTTG ATGGCAGAAG TTTCCGCAGC GGCTACAGTG AGAGGAGCCG GCACAGCAGC
    CACGATATGC TCAGCCACAG CTGGGAGGAC AGCAGGGAGA GGGGGCGTCC CCATCAGCGC
    GCACAGAGCC GGGAGCGGGA GCGCAGCCGT GGCCGGAGCC TGGAACGTGG CCTGGACCAT
    GAAGACTACG GGCGCAGCCA TGAGCGCAGC CGCGGCCGGA GCCTGGAGCG CGGTCTAGAC
    CGCGACTTTG TGTCCCGGGA CTACAGCCGT GGACGGAGCA TCGACAGGGA CTACGACCGA
    GACTATGAGC GCTCCTATCA CCAAGCCTAT GAGCCTGACT ACGAAGGCCG CTACAGCCCA
    TCTTATGACC GCAGAGCCCA TCCAGAGACC CGCTATGAAC GGAGCCGCAG CCGAGAACAC
    CTACGGTCCC GAAGCCCCAC CCCCGAGTCT AGGTCCCGGC AGGAACACAA GGGCCAGCAT
    GATCCCGACA GGCCCATCGG GGTCCTTCTG ACCAAAAGCA AAGCAAACGA AGAGTACGGG
    CTCCGGCTGG GAAGTCAGAT CTTCATTAAG GAAATGACCA GAACAGGACT GGCAACAAAA
    GATGGCAACC TTCATGAGGG GGACATAATT CTCAAGATCA ATGGAACTGT TACTGAGAAC
    ATGTCTTTAA CCGATGCTCG GAAGTTAATA GAAAAGTCTC GAGGAAAACT GCAGCTGGTG
    GTGTTGAGAG ACAGCAAGCA GACCCTCATC AACATCCCAT CTCTGAATGA CAGCGACTCG
    GAAGTGGAGG ATATCTCGGA AATCGAGTCC AACCGATCTT TTTCTCCAGA GGAGAGGCGC
    CAGCAGTATT CTGATCAGGA GTATCATTCC TCCACTGAGA AGCTGAAGGA GAGGCCAAGC
    TCAAGAGAGG AGACCTCAGG CAGAATGTCC AGGATGGGTG CCACACCCAC GCCGTTCAAG
    TCCACGGGGG ACATCACAGC TGCAGGTGTC ACAGAGGCCA ACAAGGAGCC CAGGTCCCAG
    GAGGAATCCC CAGTTCCTCA ACCCAGAACA GCATCAAGAG TCTTTCTTCG TCCTAGTCCC
    GAAGATGAAG CAATATATGG CCCTAACACC AAAATGGTGA AGTTCAAGAA GGGAGACAGC
    GTGGGCCTCC GGTTGGCTGG TGGAAATGAT GTTGGCATAT TTGTGGCTGG CATTCAGGAG
    GGCACCTCTG CAGAGCAGGA GGGCCTACAA GAAGGGGACC AGATTCTGAA GGTGAACACA
    CAAGATTTCA GAGGGCTGGT CCGGGAAGAT GCCGTCCTCT ACCTGTTAGA AATCCCTAAA
    GGTGAAACCG TGACCATTTT GGCTCAGAGC CGAGCAGACG TGTATAGAGA CATCCTGGCC
    TGTGGCAGGG GAGACTCGTT CTTCATAAGG AGCCACTTTG AATGTGAGAA GGAGACTCCG
    CAGAGCTTGG CCTTCACCAG GGGAGAGGTC TTCCGAGTGG TAGACACGCT GTACGATGGC
    AAACTGGGCC ACTGGCTGGC TGTGAGGATT GGAAATGAGC TGGAGAAGGG CTTAATCCCT
    AACAAAAGCA GAGCTGAGCA GATGGCCAGT GTCCAGAATG CCCAGCGAGA GAATGCTGGG
    GACAGAGCAG ACTTCTGGCG GATGCGTGGC CAGAGATCCG GGGTCAAGAA GAACATTCGC
    AAGAGCCGGG AAGACCTGGC AGCTGCTGTG TCGGTTAGCA CCAAGTTCCC CGCCTACGAG
    AAGGTTCTGC TTCGGGAAGC TGGCTTCAAG AGACCCGTGG TTCTGTTTGG CCCCATAGCA
    GATATAGCAA TGGAAAGGCT GACTACTGAG CTACCCGACC TGTTTCAAAC TGCAAAAACA
    GAACCCAAAG ATGCGGGATC TGAGAAATCC AGTGGAGTGG TTCGGTTGAA TACTGTGAGG
    CAAATTATTG AGCAGGACAA GCATGCCCTG CTCGACGTTA CCCCCAAAGC TGTGGACCTG
    CTCAATTATA CTCAGTGGTT CCCAATCGTG ATTTTCTTCA ACCCGGATTC CAGACAAGGC
    ATTAAAACCA TAAGGCAGAG GTTGAACCCA ACATCCAATA AAAGTTCTCG CAAGTTATTC
    GATCAAGCCA ACAAGCTCAA AAAAACCTGT TCTCATCTTT TTACAGCTAC CATCAACGTG
    AATTCAGCCA ATGATGGCTG GTTTGGCAGC CTGAAGGACA GCATTCAGCA GCAGCAAAAC
    GAAGCAGTGT GGGTCTCTGA AGGAAAGATG GAGGGGATGG ATGATGACGC TGAAGACCGC
    ATGTCCTACT TAACCGCCAT GGGTGCGGAC TATCTGAGTT GTGACAGCCG TCTCATCAGT
    GACTTTGAAG ATACGGACGG CGAGGGAGGC GCCTACACTG ACAATGAGCT GGATGAGCCA
    GCTGAGGAGC CGCTGGTGTC TTCCATCACC CGCTCCTCAG AGCCGGTGCA GCATGAGGAG
    AGCATAAGGA AGCCCAGCCC AGAGCCACGC GCTCAGATGA GGAGGGCAGC TAGCAGAGAC
    CAGCTTAGGG ATGGTAGCCC GCCCCCAGCA TTCAAGCCAG AGCCGCCCAA GGTCAGAAAC
    CAAAACAGAG AGGACTCTTT CAACTACTCC AAGTCAAACC TCTCTGCCAT GGCTGGCAGT
    GAAATCCCGG GGGGATCCAC CAAAGGGTGT CCTCCCCCTA TTGCGGTGAA ACCTGCCTTT
    GGGCGATCCA TCCTGAAGCC TTCTACTCCA GTCCCCATGC CTGAGAGTGA GGAGGTTGGG
    GAGAGCACCG AGGAGCAGGA AGAGGCTCCC AAATCAGTCC TGGGCAGAGT GAAAATCTTC
    GAGAAGATGG ACCACAAGGC GAAATTACAG AGGATGCAGG AGCTCCAAGA AGCACAGAAT
    GCGAGGATTG AAATAGCTCA GAAGCATCCT GACATCTATG CGGTTCCAAT CAAAGCCCCC
    AAGCCAGATG CTGGCCTGCC CCAGCACATG AGTTCTAGAC CCCCAGAGCC ACAGAAAGCT
    CCTTCTAGGC TTTACCAGGA CACCAGAGGA AGCTACGGCA GTGATCCCGA GGAAGAGGAG
    GAGTACCGCC AACAGTTGGC AGCACACTCG AAGCGTGGTT ACTACAGCCA GCCCTCCCGG
    TACCGAGACA CCGAATTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008758524.1
    CDS155..3655
    Translation

    Target ORF information:

    RefSeq Version XM_008760302.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008760302.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAGGAGG TGATATGGGA ACAGTACACT GTGACCCTGC AGAAGGATTC CAAAAGAGGA 
    TTTGGAATCG CAGTGTCAGG AGGCAGAGAC AACCCCCACT TTGAAAATGG GGAGACGTCA
    ATCGTCATTT CAGATGTACT CCCAGGTGGA CCCGCTGATG GCTTGCTTCA AGAAAATGAC
    AGGGTGGTCA TGGTCAATGG CACCCCCATG GAGGATGTGC TCCATTCATT TGCCGTTCAG
    CAGCTTAGGA AAAGTGGGAA GATCGCAGCC ATTGTGGTCA AGAGGCCTCG GAAGGTTCAG
    GTGGCCCCGC TGCAGGGCAG CCCCCCTCTC AGTCATGATG ACCGAGGTTT TGAGATGATG
    GATGAATTTG ATGGCAGAAG TTTCCGCAGC GGCTACAGTG AGAGGAGCCG GCACAGCAGC
    CACGATATGC TCAGCCACAG CTGGGAGGAC AGCAGGGAGA GGGGGCGTCC CCATCAGCGC
    GCACAGAGCC GGGAGCGGGA GCGCAGCCGT GGCCGGAGCC TGGAACGTGG CCTGGACCAT
    GAAGACTACG GGCGCAGCCA TGAGCGCAGC CGCGGCCGGA GCCTGGAGCG CGGTCTAGAC
    CGCGACTTTG TGTCCCGGGA CTACAGCCGT GGACGGAGCA TCGACAGGGA CTACGACCGA
    GACTATGAGC GCTCCTATCA CCAAGCCTAT GAGCCTGACT ACGAAGGCCG CTACAGCCCA
    TCTTATGACC GCAGAGCCCA TCCAGAGACC CGCTATGAAC GGAGCCGCAG CCGAGAACAC
    CTACGGTCCC GAAGCCCCAC CCCCGAGTCT AGGTCCCGGC AGGAACACAA GGGCCAGCAT
    GATCCCGACA GGCCCATCGG GGTCCTTCTG ACCAAAAGCA AAGCAAACGA AGAGTACGGG
    CTCCGGCTGG GAAGTCAGAT CTTCATTAAG GAAATGACCA GAACAGGACT GGCAACAAAA
    GATGGCAACC TTCATGAGGG GGACATAATT CTCAAGATCA ATGGAACTGT TACTGAGAAC
    ATGTCTTTAA CCGATGCTCG GAAGTTAATA GAAAAGTCTC GAGGAAAACT GCAGCTGGTG
    GTGTTGAGAG ACAGCAAGCA GACCCTCATC AACATCCCAT CTCTGAATGA CAGCGACTCG
    GAAGTGGAGG ATATCTCGGA AATCGAGTCC AACCGATCTT TTTCTCCAGA GGAGAGGCGC
    CAGCAGTATT CTGATCAGGA GTATCATTCC TCCACTGAGA AGCTGAAGGA GAGGCCAAGC
    TCAAGAGAGG AGACCTCAGG CAGAATGTCC AGGATGGGTG CCACACCCAC GCCGTTCAAG
    TCCACGGGGG ACATCACAGC TGCAGGTGTC ACAGAGGCCA ACAAGGAGCC CAGGTCCCAG
    GAGGAATCCC CAGTTCCTCA ACCCAGAACA GCATCAAGAG TCTTTCTTCG TCCTAGTCCC
    GAAGATGAAG CAATATATGG CCCTAACACC AAAATGGTGA AGTTCAAGAA GGGAGACAGC
    GTGGGCCTCC GGTTGGCTGG TGGAAATGAT GTTGGCATAT TTGTGGCTGG CATTCAGGAG
    GGCACCTCTG CAGAGCAGGA GGGCCTACAA GAAGGGGACC AGATTCTGAA GGTGAACACA
    CAAGATTTCA GAGGGCTGGT CCGGGAAGAT GCCGTCCTCT ACCTGTTAGA AATCCCTAAA
    GGTGAAACCG TGACCATTTT GGCTCAGAGC CGAGCAGACG TGTATAGAGA CATCCTGGCC
    TGTGGCAGGG GAGACTCGTT CTTCATAAGG AGCCACTTTG AATGTGAGAA GGAGACTCCG
    CAGAGCTTGG CCTTCACCAG GGGAGAGGTC TTCCGAGTGG TAGACACGCT GTACGATGGC
    AAACTGGGCC ACTGGCTGGC TGTGAGGATT GGAAATGAGC TGGAGAAGGG CTTAATCCCT
    AACAAAAGCA GAGCTGAGCA GATGGCCAGT GTCCAGAATG CCCAGCGAGA GAATGCTGGG
    GACAGAGCAG ACTTCTGGCG GATGCGTGGC CAGAGATCCG GGGTCAAGAA GAACATTCGC
    AAGAGCCGGG AAGACCTGGC AGCTGCTGTG TCGGTTAGCA CCAAGTTCCC CGCCTACGAG
    AAGGTTCTGC TTCGGGAAGC TGGCTTCAAG AGACCCGTGG TTCTGTTTGG CCCCATAGCA
    GATATAGCAA TGGAAAGGCT GACTACTGAG CTACCCGACC TGTTTCAAAC TGCAAAAACA
    GAACCCAAAG ATGCGGGATC TGAGAAATCC AGTGGAGTGG TTCGGTTGAA TACTGTGAGG
    CAAATTATTG AGCAGGACAA GCATGCCCTG CTCGACGTTA CCCCCAAAGC TGTGGACCTG
    CTCAATTATA CTCAGTGGTT CCCAATCGTG ATTTTCTTCA ACCCGGATTC CAGACAAGGC
    ATTAAAACCA TAAGGCAGAG GTTGAACCCA ACATCCAATA AAAGTTCTCG CAAGTTATTC
    GATCAAGCCA ACAAGCTCAA AAAAACCTGT TCTCATCTTT TTACAGCTAC CATCAACGTG
    AATTCAGCCA ATGATGGCTG GTTTGGCAGC CTGAAGGACA GCATTCAGCA GCAGCAAAAC
    GAAGCAGTGT GGGTCTCTGA AGGAAAGATG GAGGGGATGG ATGATGACGC TGAAGACCGC
    ATGTCCTACT TAACCGCCAT GGGTGCGGAC TATCTGAGTT GTGACAGCCG TCTCATCAGT
    GACTTTGAAG ATACGGACGG CGAGGGAGGC GCCTACACTG ACAATGAGCT GGATGAGCCA
    GCTGAGGAGC CGCTGGTGTC TTCCATCACC CGCTCCTCAG AGCCGGTGCA GCATGAGGAG
    AGCATAAGGA AGCCCAGCCC AGAGCCACGC GCTCAGATGA GGAGGGCAGC TAGCAGAGAC
    CAGCTTAGGG ATGGTAGCCC GCCCCCAGCA TTCAAGCCAG AGCCGCCCAA GGTCAGAAAC
    CAAAACAGAG AGGACTCTTT CAACTACTCC AAGTCAAACC TCTCTGCCAT GGCTGGCAGT
    GAAATCCCGG GGGGATCCAC CAAAGGGTGT CCTCCCCCTA TTGCGGTGAA ACCTGCCTTT
    GGGCGATCCA TCCTGAAGCC TTCTACTCCA GTCCCCATGC CTGAGAGTGA GGAGGTTGGG
    GAGAGCACCG AGGAGCAGGA AGAGGCTCCC AAATCAGTCC TGGGCAGAGT GAAAATCTTC
    GAGAAGATGG ACCACAAGGC GAAATTACAG AGGATGCAGG AGCTCCAAGA AGCACAGAAT
    GCGAGGATTG AAATAGCTCA GAAGCATCCT GACATCTATG CGGTTCCAAT CAAAGCCCCC
    AAGCCAGATG CTGGCCTGCC CCAGCACATG AGTTCTAGAC CCCCAGAGCC ACAGAAAGCT
    CCTTCTAGGC TTTACCAGGA CACCAGAGGA AGCTACGGCA GTGATCCCGA GGAAGAGGAG
    GAGTACCGCC AACAGTTGGC AGCACACTCG AAGCGTGGTT ACTACAGCCA GCCCTCCCGG
    TACCGAGACA CCGAATTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa23978
    Clone ID Related Accession (Same CDS sequence) XM_006231182.3
    Accession Version XM_006231182.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product tight junction protein ZO-2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231182.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGGGCTG CTCAGGTCTT CCAGAGGATG TGGATCCAGG CAGTTAAAAA GTTGGAGAGA 
    TGGAAAGGCC ATGGCCCAGG CATGGAGGAG GTGATATGGG AACAGTACAC TGTGACCCTG
    CAGAAGGATT CCAAAAGAGG ATTTGGAATC GCAGTGTCAG GAGGCAGAGA CAACCCCCAC
    TTTGAAAATG GGGAGACGTC AATCGTCATT TCAGATGTAC TCCCAGGTGG ACCCGCTGAT
    GGCTTGCTTC AAGAAAATGA CAGGGTGGTC ATGGTCAATG GCACCCCCAT GGAGGATGTG
    CTCCATTCAT TTGCCGTTCA GCAGCTTAGG AAAAGTGGGA AGATCGCAGC CATTGTGGTC
    AAGAGGCCTC GGAAGGTTCA GGTGGCCCCG CTGCAGGGCA GCCCCCCTCT CAGTCATGAT
    GACCGAGGTT TTGAGATGAT GGATGAATTT GATGGCAGAA GTTTCCGCAG CGGCTACAGT
    GAGAGGAGCC GGCACAGCAG CCACGATATG CTCAGCCACA GCTGGGAGGA CAGCAGGGAG
    AGGGGGCGTC CCCATCAGCG CGCACAGAGC CGGGAGCGGG AGCGCAGCCG TGGCCGGAGC
    CTGGAACGTG GCCTGGACCA TGAAGACTAC GGGCGCAGCC ATGAGCGCAG CCGCGGCCGG
    AGCCTGGAGC GCGGTCTAGA CCGCGACTTT GTGTCCCGGG ACTACAGCCG TGGACGGAGC
    ATCGACAGGG ACTACGACCG AGACTATGAG CGCTCCTATC ACCAAGCCTA TGAGCCTGAC
    TACGAAGGCC GCTACAGCCC ATCTTATGAC CGCAGAGCCC ATCCAGAGAC CCGCTATGAA
    CGGAGCCGCA GCCGAGAACA CCTACGGTCC CGAAGCCCCA CCCCCGAGTC TAGGTCCCGG
    CAGGAACACA AGGGCCAGCA TGATCCCGAC AGGCCCATCG GGGTCCTTCT GACCAAAAGC
    AAAGCAAACG AAGAGTACGG GCTCCGGCTG GGAAGTCAGA TCTTCATTAA GGAAATGACC
    AGAACAGGAC TGGCAACAAA AGATGGCAAC CTTCATGAGG GGGACATAAT TCTCAAGATC
    AATGGAACTG TTACTGAGAA CATGTCTTTA ACCGATGCTC GGAAGTTAAT AGAAAAGTCT
    CGAGGAAAAC TGCAGCTGGT GGTGTTGAGA GACAGCAAGC AGACCCTCAT CAACATCCCA
    TCTCTGAATG ACAGCGACTC GGAAGTGGAG GATATCTCGG AAATCGAGTC CAACCGATCT
    TTTTCTCCAG AGGAGAGGCG CCAGCAGTAT TCTGATCAGG AGTATCATTC CTCCACTGAG
    AAGCTGAAGG AGAGGCCAAG CTCAAGAGAG GAGACCTCAG GCAGAATGTC CAGGATGGGT
    GCCACACCCA CGCCGTTCAA GTCCACGGGG GACATCACAG CTGCAGGTGT CACAGAGGCC
    AACAAGGAGC CCAGGTCCCA GGAGGAATCC CCAGTTCCTC AACCCAGAAC AGCATCAAGA
    GTCTTTCTTC GTCCTAGTCC CGAAGATGAA GCAATATATG GCCCTAACAC CAAAATGGTG
    AAGTTCAAGA AGGGAGACAG CGTGGGCCTC CGGTTGGCTG GTGGAAATGA TGTTGGCATA
    TTTGTGGCTG GCATTCAGGA GGGCACCTCT GCAGAGCAGG AGGGCCTACA AGAAGGGGAC
    CAGATTCTGA AGGTGAACAC ACAAGATTTC AGAGGGCTGG TCCGGGAAGA TGCCGTCCTC
    TACCTGTTAG AAATCCCTAA AGGTGAAACC GTGACCATTT TGGCTCAGAG CCGAGCAGAC
    GTGTATAGAG ACATCCTGGC CTGTGGCAGG GGAGACTCGT TCTTCATAAG GAGCCACTTT
    GAATGTGAGA AGGAGACTCC GCAGAGCTTG GCCTTCACCA GGGGAGAGGT CTTCCGAGTG
    GTAGACACGC TGTACGATGG CAAACTGGGC CACTGGCTGG CTGTGAGGAT TGGAAATGAG
    CTGGAGAAGG GCTTAATCCC TAACAAAAGC AGAGCTGAGC AGATGGCCAG TGTCCAGAAT
    GCCCAGCGAG AGAATGCTGG GGACAGAGCA GACTTCTGGC GGATGCGTGG CCAGAGATCC
    GGGGTCAAGA AGAACATTCG CAAGAGCCGG GAAGACCTGG CAGCTGCTGT GTCGGTTAGC
    ACCAAGTTCC CCGCCTACGA GAAGGTTCTG CTTCGGGAAG CTGGCTTCAA GAGACCCGTG
    GTTCTGTTTG GCCCCATAGC AGATATAGCA ATGGAAAGGC TGACTACTGA GCTACCCGAC
    CTGTTTCAAA CTGCAAAAAC AGAACCCAAA GATGCGGGAT CTGAGAAATC CAGTGGAGTG
    GTTCGGTTGA ATACTGTGAG GCAAATTATT GAGCAGGACA AGCATGCCCT GCTCGACGTT
    ACCCCCAAAG CTGTGGACCT GCTCAATTAT ACTCAGTGGT TCCCAATCGT GATTTTCTTC
    AACCCGGATT CCAGACAAGG CATTAAAACC ATAAGGCAGA GGTTGAACCC AACATCCAAT
    AAAAGTTCTC GCAAGTTATT CGATCAAGCC AACAAGCTCA AAAAAACCTG TTCTCATCTT
    TTTACAGCTA CCATCAACGT GAATTCAGCC AATGATGGCT GGTTTGGCAG CCTGAAGGAC
    AGCATTCAGC AGCAGCAAAA CGAAGCAGTG TGGGTCTCTG AAGGAAAGAT GGAGGGGATG
    GATGATGACG CTGAAGACCG CATGTCCTAC TTAACCGCCA TGGGTGCGGA CTATCTGAGT
    TGTGACAGCC GTCTCATCAG TGACTTTGAA GATACGGACG GCGAGGGAGG CGCCTACACT
    GACAATGAGC TGGATGAGCC AGCTGAGGAG CCGCTGGTGT CTTCCATCAC CCGCTCCTCA
    GAGCCGGTGC AGCATGAGGA GGTCAGAAAC CAAAACAGAG AGGACTCTTT CAACTACTCC
    AAGTCAAACC TCTCTGCCAT GGCTGGCAGT GAAATCCCGG GGGGATCCAC CAAAGGGTGT
    CCTCCCCCTA TTGCGGTGAA ACCTGCCTTT GGGCGATCCA TCCTGAAGCC TTCTACTCCA
    GTCCCCATGC CTGAGAGTGA GGAGGTTGGG GAGAGCACCG AGGAGCAGGA AGAGGCTCCC
    AAATCAGTCC TGGGCAGAGT GAAAATCTTC GAGAAGATGG ACCACAAGGC GAAATTACAG
    AGGATGCAGG AGCTCCAAGA AGCACAGAAT GCGAGGATTG AAATAGCTCA GAAGCATCCT
    GACATCTATG CGGTTCCAAT CAAAGCCCCC AAGCCAGATG CTGGCCTGCC CCAGCACATG
    AGTTCTAGAC CCCCAGAGCC ACAGAAAGCT CCTTCTAGGC TTTACCAGGA CACCAGAGGA
    AGCTACGGCA GTGATCCCGA GGAAGAGGAG GAGTACCGCC AACAGTTGGC AGCACACTCG
    AAGCGTGGTT ACTACAGCCA GCCCTCCCGG TACCGAGACA CCGAATTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006231244.1
    CDS540..4010
    Translation

    Target ORF information:

    RefSeq Version XM_006231182.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus tight junction protein 2 (Tjp2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006231182.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGGGCTG CTCAGGTCTT CCAGAGGATG TGGATCCAGG CAGTTAAAAA GTTGGAGAGA 
    TGGAAAGGCC ATGGCCCAGG CATGGAGGAG GTGATATGGG AACAGTACAC TGTGACCCTG
    CAGAAGGATT CCAAAAGAGG ATTTGGAATC GCAGTGTCAG GAGGCAGAGA CAACCCCCAC
    TTTGAAAATG GGGAGACGTC AATCGTCATT TCAGATGTAC TCCCAGGTGG ACCCGCTGAT
    GGCTTGCTTC AAGAAAATGA CAGGGTGGTC ATGGTCAATG GCACCCCCAT GGAGGATGTG
    CTCCATTCAT TTGCCGTTCA GCAGCTTAGG AAAAGTGGGA AGATCGCAGC CATTGTGGTC
    AAGAGGCCTC GGAAGGTTCA GGTGGCCCCG CTGCAGGGCA GCCCCCCTCT CAGTCATGAT
    GACCGAGGTT TTGAGATGAT GGATGAATTT GATGGCAGAA GTTTCCGCAG CGGCTACAGT
    GAGAGGAGCC GGCACAGCAG CCACGATATG CTCAGCCACA GCTGGGAGGA CAGCAGGGAG
    AGGGGGCGTC CCCATCAGCG CGCACAGAGC CGGGAGCGGG AGCGCAGCCG TGGCCGGAGC
    CTGGAACGTG GCCTGGACCA TGAAGACTAC GGGCGCAGCC ATGAGCGCAG CCGCGGCCGG
    AGCCTGGAGC GCGGTCTAGA CCGCGACTTT GTGTCCCGGG ACTACAGCCG TGGACGGAGC
    ATCGACAGGG ACTACGACCG AGACTATGAG CGCTCCTATC ACCAAGCCTA TGAGCCTGAC
    TACGAAGGCC GCTACAGCCC ATCTTATGAC CGCAGAGCCC ATCCAGAGAC CCGCTATGAA
    CGGAGCCGCA GCCGAGAACA CCTACGGTCC CGAAGCCCCA CCCCCGAGTC TAGGTCCCGG
    CAGGAACACA AGGGCCAGCA TGATCCCGAC AGGCCCATCG GGGTCCTTCT GACCAAAAGC
    AAAGCAAACG AAGAGTACGG GCTCCGGCTG GGAAGTCAGA TCTTCATTAA GGAAATGACC
    AGAACAGGAC TGGCAACAAA AGATGGCAAC CTTCATGAGG GGGACATAAT TCTCAAGATC
    AATGGAACTG TTACTGAGAA CATGTCTTTA ACCGATGCTC GGAAGTTAAT AGAAAAGTCT
    CGAGGAAAAC TGCAGCTGGT GGTGTTGAGA GACAGCAAGC AGACCCTCAT CAACATCCCA
    TCTCTGAATG ACAGCGACTC GGAAGTGGAG GATATCTCGG AAATCGAGTC CAACCGATCT
    TTTTCTCCAG AGGAGAGGCG CCAGCAGTAT TCTGATCAGG AGTATCATTC CTCCACTGAG
    AAGCTGAAGG AGAGGCCAAG CTCAAGAGAG GAGACCTCAG GCAGAATGTC CAGGATGGGT
    GCCACACCCA CGCCGTTCAA GTCCACGGGG GACATCACAG CTGCAGGTGT CACAGAGGCC
    AACAAGGAGC CCAGGTCCCA GGAGGAATCC CCAGTTCCTC AACCCAGAAC AGCATCAAGA
    GTCTTTCTTC GTCCTAGTCC CGAAGATGAA GCAATATATG GCCCTAACAC CAAAATGGTG
    AAGTTCAAGA AGGGAGACAG CGTGGGCCTC CGGTTGGCTG GTGGAAATGA TGTTGGCATA
    TTTGTGGCTG GCATTCAGGA GGGCACCTCT GCAGAGCAGG AGGGCCTACA AGAAGGGGAC
    CAGATTCTGA AGGTGAACAC ACAAGATTTC AGAGGGCTGG TCCGGGAAGA TGCCGTCCTC
    TACCTGTTAG AAATCCCTAA AGGTGAAACC GTGACCATTT TGGCTCAGAG CCGAGCAGAC
    GTGTATAGAG ACATCCTGGC CTGTGGCAGG GGAGACTCGT TCTTCATAAG GAGCCACTTT
    GAATGTGAGA AGGAGACTCC GCAGAGCTTG GCCTTCACCA GGGGAGAGGT CTTCCGAGTG
    GTAGACACGC TGTACGATGG CAAACTGGGC CACTGGCTGG CTGTGAGGAT TGGAAATGAG
    CTGGAGAAGG GCTTAATCCC TAACAAAAGC AGAGCTGAGC AGATGGCCAG TGTCCAGAAT
    GCCCAGCGAG AGAATGCTGG GGACAGAGCA GACTTCTGGC GGATGCGTGG CCAGAGATCC
    GGGGTCAAGA AGAACATTCG CAAGAGCCGG GAAGACCTGG CAGCTGCTGT GTCGGTTAGC
    ACCAAGTTCC CCGCCTACGA GAAGGTTCTG CTTCGGGAAG CTGGCTTCAA GAGACCCGTG
    GTTCTGTTTG GCCCCATAGC AGATATAGCA ATGGAAAGGC TGACTACTGA GCTACCCGAC
    CTGTTTCAAA CTGCAAAAAC AGAACCCAAA GATGCGGGAT CTGAGAAATC CAGTGGAGTG
    GTTCGGTTGA ATACTGTGAG GCAAATTATT GAGCAGGACA AGCATGCCCT GCTCGACGTT
    ACCCCCAAAG CTGTGGACCT GCTCAATTAT ACTCAGTGGT TCCCAATCGT GATTTTCTTC
    AACCCGGATT CCAGACAAGG CATTAAAACC ATAAGGCAGA GGTTGAACCC AACATCCAAT
    AAAAGTTCTC GCAAGTTATT CGATCAAGCC AACAAGCTCA AAAAAACCTG TTCTCATCTT
    TTTACAGCTA CCATCAACGT GAATTCAGCC AATGATGGCT GGTTTGGCAG CCTGAAGGAC
    AGCATTCAGC AGCAGCAAAA CGAAGCAGTG TGGGTCTCTG AAGGAAAGAT GGAGGGGATG
    GATGATGACG CTGAAGACCG CATGTCCTAC TTAACCGCCA TGGGTGCGGA CTATCTGAGT
    TGTGACAGCC GTCTCATCAG TGACTTTGAA GATACGGACG GCGAGGGAGG CGCCTACACT
    GACAATGAGC TGGATGAGCC AGCTGAGGAG CCGCTGGTGT CTTCCATCAC CCGCTCCTCA
    GAGCCGGTGC AGCATGAGGA GGTCAGAAAC CAAAACAGAG AGGACTCTTT CAACTACTCC
    AAGTCAAACC TCTCTGCCAT GGCTGGCAGT GAAATCCCGG GGGGATCCAC CAAAGGGTGT
    CCTCCCCCTA TTGCGGTGAA ACCTGCCTTT GGGCGATCCA TCCTGAAGCC TTCTACTCCA
    GTCCCCATGC CTGAGAGTGA GGAGGTTGGG GAGAGCACCG AGGAGCAGGA AGAGGCTCCC
    AAATCAGTCC TGGGCAGAGT GAAAATCTTC GAGAAGATGG ACCACAAGGC GAAATTACAG
    AGGATGCAGG AGCTCCAAGA AGCACAGAAT GCGAGGATTG AAATAGCTCA GAAGCATCCT
    GACATCTATG CGGTTCCAAT CAAAGCCCCC AAGCCAGATG CTGGCCTGCC CCAGCACATG
    AGTTCTAGAC CCCCAGAGCC ACAGAAAGCT CCTTCTAGGC TTTACCAGGA CACCAGAGGA
    AGCTACGGCA GTGATCCCGA GGAAGAGGAG GAGTACCGCC AACAGTTGGC AGCACACTCG
    AAGCGTGGTT ACTACAGCCA GCCCTCCCGG TACCGAGACA CCGAATTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.