Mapre1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mapre1
Entrez Gene ID	114764
Full Name	microtubule-associated protein, RP/EB family, member 1
Synonyms	Eb1
General protein information	Preferred Names microtubule-associated protein, RP/EB family, member 1 Names microtubule-associated protein RP/EB family member 1 APC-binding protein EB1 adenomatosis polyposis coli binding protein Eb1 end-binding protein 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q41
Summary	expressed specifically at growing microtubule distal tips [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006235295.3	XP_006235357.1	microtubule-associated protein RP/EB family member 1 isoform X1
NM_138509.3	NP_612518.2	microtubule-associated protein RP/EB family member 1
XM_006235294.3	XP_006235356.1	microtubule-associated protein RP/EB family member 1 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MAPRE1	NP_001238875.1
Neurospora crassa	NCU00243	XP_957714.2
Oryza sativa (rice)	Os10g0498900	NP_001064972.1
Xenopus tropicalis (tropical clawed frog)	mapre1	NP_989115.1
Mus musculus (house mouse)	Mapre1	NP_031922.1
Rattus norvegicus (Norway rat)	Mapre1	NP_612518.2
Saccharomyces cerevisiae (baker's yeast)	BIM1	NP_010932.1
Schizosaccharomyces pombe (fission yeast)	mal3	NP_593678.1
Arabidopsis thaliana (thale cress)	EB1C	NP_201528.1
Kluyveromyces lactis	KLLA0B11550g	XP_452044.1
Magnaporthe oryzae (rice blast fungus)	MGG_05427	XP_003710287.1
Pan troglodytes (chimpanzee)	MAPRE1	XP_001154462.2
Gallus gallus (chicken)	MAPRE1	NP_001026031.1
Homo sapiens (human)	MAPRE1	NP_036457.1
	MAPRE1	XP_001107004.2
Bos taurus (cattle)	MAPRE1	NP_001068802.1
Danio rerio (zebrafish)	mapre1b	NP_998805.1
Eremothecium gossypii	AGOS_AAR024W	NP_982565.1

Gene Ontology
Bibliography

Related articles in PubMed

EB1-binding-myomegalin protein complex promotes centrosomal microtubules functions.
Bouguenina H, Salaun D, Mangon A, Muller L, Baudelet E, Camoin L, Tachibana T, Cianf?rani S, Audebert S, Verdier-Pinard P, Badache A
Proceedings of the National Academy of Sciences of the United States of America114(50)E10687-E10696(2017 Dec)

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Yachie N, Petsalaki E, Mellor JC, Weile J, Jacob Y, Verby M, Ozturk SB, Li S, Cote AG, Mosca R, Knapp JJ, Ko M, Yu A, Gebbia M, Sahni N, Yi S, Tyagi T, Sheykhkarimli D, Roth JF, Wong C, Musa L, Snider J, Liu YC, Yu H, Braun P, Stagljar I, Hao T, Calderwood MA, Pelletier L, Aloy P, Hill DE, Vidal M, Roth FP
Molecular systems biology12(4)863(2016 Apr)

Signaling Scaffold Protein IQGAP1 Interacts with Microtubule Plus-end Tracking Protein SKAP and Links Dynamic Microtubule Plus-end to Steer Cell Migration.
Cao D, Su Z, Wang W, Wu H, Liu X, Akram S, Qin B, Zhou J, Zhuang X, Adams G, Jin C, Wang X, Liu L, Hill DL, Wang D, Ding X, Yao X
The Journal of biological chemistry290(39)23766-80(2015 Sep)

TTBK2 with EB1/3 regulates microtubule dynamics in migrating cells through KIF2A phosphorylation.
Watanabe T, Kakeno M, Matsui T, Sugiyama I, Arimura N, Matsuzawa K, Shirahige A, Ishidate F, Nishioka T, Taya S, Hoshino M, Kaibuchi K
The Journal of cell biology210(5)737-51(2015 Aug)

Microtubules regulate focal adhesion dynamics through MAP4K4.
Yue J, Xie M, Gou X, Lee P, Schneider MD, Wu X
Developmental cell31(5)572-85(2014 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

provides a functional link between tubulin- and actin-based cytoskeletons to confer spermatocyte transport at the blood-testis barrier
Title: EB1 regulates tubulin and actin cytoskeletal networks at the sertoli cell blood-testis barrier in male rats: an in vitro study.

the Amer2-EB1-APC complex regulates cell migration by altering microtubule stability.
Title: Amer2 protein interacts with EB1 protein and adenomatous polyposis coli (APC) and controls microtubule stability and cell migration.

EB3 and EB1 coordinate a molecular and functional interplay between ankyrin G (ankG)and the axon initial segment microtubules that supports the central role of ankG in the maintenance of neuronal polarity
Title: End-binding proteins EB3 and EB1 link microtubules to ankyrin G in the axon initial segment.

In this study we used EB1 immunostaining in mitotic NRK-52E cells to quantitatively analyse the length and number of growing astral microtubules during metaphase and anaphase
Title: EB 1 immunofluorescence reveals an increase in growing astral microtubule length and number during anaphase in NRK-52E cells.

The following Mapre1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mapre1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa01491	NM_138509.3 Latest version!	Rattus norvegicus microtubule-associated protein, RP/EB family, member 1 (Mapre1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa01491	XM_006235295.3 Latest version!	Rattus norvegicus microtubule-associated protein, RP/EB family, member 1 (Mapre1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa01491	XM_006235294.3 Latest version!	Rattus norvegicus microtubule-associated protein, RP/EB family, member 1 (Mapre1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mapre1 ( NM_138509.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_612518.2 Rattus norvegicus microtubule-associated protein, RP/EB family, member 1 (Mapre1), mRNA.

Price & Availability↑

CloneID	ORa01491
Clone ID Related Accession (Same CDS sequence)	XM_006235295.3 , NM_138509.3 , XM_006235294.3
Accession Version	NM_138509.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	microtubule-associated protein RP/EB family member 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC081726.1. On Oct 25, 2005 this sequence version replaced NM_138509.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC081726.1, CF110918.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGTGA ATGTGTACTC GACGTCAGTC ACCAGTGATA ACCTCAGTCG ACATGACATG 
CTGGCTTGGA TCAATGAATC TCTGCAGTTG AATCTGACAA AGATAGAACA GCTATGTTCA 
GGGGCTGCAT ACTGTCAGTT CATGGACATG CTCTTCCCCG GCTCCATTGC CTTGAAGAAA 
GTGAAATTCC AGGCTAAGCT AGAACATGAA TATATCCAGA ACTTCAAAAT ACTACAAGCC 
GGCTTCAAGA GGATGGGCGT TGACAAAATA ATTCCTGTGG ATAAATTAGT AAAAGGAAAA 
TTTCAGGACA ATTTTGAATT CGTTCAATGG TTCAAGAAGT TTTTTGATGC AAATTATGAT 
GGAAAAGAAT ATGATCCTGT CGCTGCCAGA CAAGGTCAAG AAACGGCAGT GGCTCCTTCT 
CTTGTCGCTC CAGCTTTGAG TAAACCGAAG AAACCTCTCG GCTCCGGTAG TGCAGCCCCA 
CAGAGACCCA TTGCAACACA GAGGACTACT GCAGCTCCTA AGGCTGGCCC CGGGATGGTG 
CGAAAGAATC CTGGCATGGG CAATGGAGAT GACGAAGCTG CTGAATTGAT GCAGCAGGTC 
AAAGTATTGA AGCTTACTGT TGAAGACTTG GAGAAGGAGA GAGATTTCTA CTTCGGCAAG 
CTAAGGAACA TTGAATTGAT TTGCCAGGAG AATGAGGGGG AAAATGACCC TGTACTGCAG 
AGGATTGTAG ATATTCTTTA TGCCACAGAT GAAGGCTTTG TGATACCTGA CGAGGGGGGC 
CCACAGGAGG AACAAGAAGA GTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_612518.2
CDS	81..887
Translation	MAVNVYSTSVTSDNLSRHDMLAWINESLQLNLTKIEQLCSGAAYCQFMDMLFPGSIALKKVKFQAKLEHEYIQNFKILQAGFKRMGVDKIIPVDKLVKGKFQDNFEFVQWFKKFFDANYDGKEYDPVAARQGQETAVAPSLVAPALSKPKKPLGSGSAAPQRPIATQRTTAAPKAGPGMVRKNPGMGNGDDEAAELMQQVKVLKLTVEDLEKERDFYFGKLRNIELICQENEGENDPVLQRIVDILYATDEGFVIPDEGGPQEEQEEY

Target ORF information:

RefSeq Version	NM_138509.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus microtubule-associated protein, RP/EB family, member 1 (Mapre1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138509.3

ORF Insert Sequence:

ATGGCAGTGA ATGTGTACTC GACGTCAGTC ACCAGTGATA ACCTCAGTCG ACATGACATG 
CTGGCTTGGA TCAATGAATC TCTGCAGTTG AATCTGACAA AGATAGAACA GCTATGTTCA 
GGGGCTGCAT ACTGTCAGTT CATGGACATG CTCTTCCCCG GCTCCATTGC CTTGAAGAAA 
GTGAAATTCC AGGCTAAGCT AGAACATGAA TATATCCAGA ACTTCAAAAT ACTACAAGCC 
GGCTTCAAGA GGATGGGCGT TGACAAAATA ATTCCTGTGG ATAAATTAGT AAAAGGAAAA 
TTTCAGGACA ATTTTGAATT CGTTCAATGG TTCAAGAAGT TTTTTGATGC AAATTATGAT 
GGAAAAGAAT ATGATCCTGT CGCTGCCAGA CAAGGTCAAG AAACGGCAGT GGCTCCTTCT 
CTTGTCGCTC CAGCTTTGAG TAAACCGAAG AAACCTCTCG GCTCCGGTAG TGCAGCCCCA 
CAGAGACCCA TTGCAACACA GAGGACTACT GCAGCTCCTA AGGCTGGCCC CGGGATGGTG 
CGAAAGAATC CTGGCATGGG CAATGGAGAT GACGAAGCTG CTGAATTGAT GCAGCAGGTC 
AAAGTATTGA AGCTTACTGT TGAAGACTTG GAGAAGGAGA GAGATTTCTA CTTCGGCAAG 
CTAAGGAACA TTGAATTGAT TTGCCAGGAG AATGAGGGGG AAAATGACCC TGTACTGCAG 
AGGATTGTAG ATATTCTTTA TGCCACAGAT GAAGGCTTTG TGATACCTGA CGAGGGGGGC 
CCACAGGAGG AACAAGAAGA GTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mapre1 ( XM_006235295.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006235357.1 Rattus norvegicus microtubule-associated protein, RP/EB family, member 1 (Mapre1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa01491
Clone ID Related Accession (Same CDS sequence)	XM_006235295.3 , NM_138509.3 , XM_006235294.3
Accession Version	XM_006235295.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	microtubule-associated protein RP/EB family member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006235295.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGTGA ATGTGTACTC GACGTCAGTC ACCAGTGATA ACCTCAGTCG ACATGACATG 
CTGGCTTGGA TCAATGAATC TCTGCAGTTG AATCTGACAA AGATAGAACA GCTATGTTCA 
GGGGCTGCAT ACTGTCAGTT CATGGACATG CTCTTCCCCG GCTCCATTGC CTTGAAGAAA 
GTGAAATTCC AGGCTAAGCT AGAACATGAA TATATCCAGA ACTTCAAAAT ACTACAAGCC 
GGCTTCAAGA GGATGGGCGT TGACAAAATA ATTCCTGTGG ATAAATTAGT AAAAGGAAAA 
TTTCAGGACA ATTTTGAATT CGTTCAATGG TTCAAGAAGT TTTTTGATGC AAATTATGAT 
GGAAAAGAAT ATGATCCTGT CGCTGCCAGA CAAGGTCAAG AAACGGCAGT GGCTCCTTCT 
CTTGTCGCTC CAGCTTTGAG TAAACCGAAG AAACCTCTCG GCTCCGGTAG TGCAGCCCCA 
CAGAGACCCA TTGCAACACA GAGGACTACT GCAGCTCCTA AGGCTGGCCC CGGGATGGTG 
CGAAAGAATC CTGGCATGGG CAATGGAGAT GACGAAGCTG CTGAATTGAT GCAGCAGGTC 
AAAGTATTGA AGCTTACTGT TGAAGACTTG GAGAAGGAGA GAGATTTCTA CTTCGGCAAG 
CTAAGGAACA TTGAATTGAT TTGCCAGGAG AATGAGGGGG AAAATGACCC TGTACTGCAG 
AGGATTGTAG ATATTCTTTA TGCCACAGAT GAAGGCTTTG TGATACCTGA CGAGGGGGGC 
CCACAGGAGG AACAAGAAGA GTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006235357.1
CDS	141..947
Translation	MAVNVYSTSVTSDNLSRHDMLAWINESLQLNLTKIEQLCSGAAYCQFMDMLFPGSIALKKVKFQAKLEHEYIQNFKILQAGFKRMGVDKIIPVDKLVKGKFQDNFEFVQWFKKFFDANYDGKEYDPVAARQGQETAVAPSLVAPALSKPKKPLGSGSAAPQRPIATQRTTAAPKAGPGMVRKNPGMGNGDDEAAELMQQVKVLKLTVEDLEKERDFYFGKLRNIELICQENEGENDPVLQRIVDILYATDEGFVIPDEGGPQEEQEEY

Target ORF information:

RefSeq Version	XM_006235295.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus microtubule-associated protein, RP/EB family, member 1 (Mapre1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006235295.3

ORF Insert Sequence:

ATGGCAGTGA ATGTGTACTC GACGTCAGTC ACCAGTGATA ACCTCAGTCG ACATGACATG 
CTGGCTTGGA TCAATGAATC TCTGCAGTTG AATCTGACAA AGATAGAACA GCTATGTTCA 
GGGGCTGCAT ACTGTCAGTT CATGGACATG CTCTTCCCCG GCTCCATTGC CTTGAAGAAA 
GTGAAATTCC AGGCTAAGCT AGAACATGAA TATATCCAGA ACTTCAAAAT ACTACAAGCC 
GGCTTCAAGA GGATGGGCGT TGACAAAATA ATTCCTGTGG ATAAATTAGT AAAAGGAAAA 
TTTCAGGACA ATTTTGAATT CGTTCAATGG TTCAAGAAGT TTTTTGATGC AAATTATGAT 
GGAAAAGAAT ATGATCCTGT CGCTGCCAGA CAAGGTCAAG AAACGGCAGT GGCTCCTTCT 
CTTGTCGCTC CAGCTTTGAG TAAACCGAAG AAACCTCTCG GCTCCGGTAG TGCAGCCCCA 
CAGAGACCCA TTGCAACACA GAGGACTACT GCAGCTCCTA AGGCTGGCCC CGGGATGGTG 
CGAAAGAATC CTGGCATGGG CAATGGAGAT GACGAAGCTG CTGAATTGAT GCAGCAGGTC 
AAAGTATTGA AGCTTACTGT TGAAGACTTG GAGAAGGAGA GAGATTTCTA CTTCGGCAAG 
CTAAGGAACA TTGAATTGAT TTGCCAGGAG AATGAGGGGG AAAATGACCC TGTACTGCAG 
AGGATTGTAG ATATTCTTTA TGCCACAGAT GAAGGCTTTG TGATACCTGA CGAGGGGGGC 
CCACAGGAGG AACAAGAAGA GTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mapre1 ( XM_006235294.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006235356.1 Rattus norvegicus microtubule-associated protein, RP/EB family, member 1 (Mapre1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa01491
Clone ID Related Accession (Same CDS sequence)	XM_006235295.3 , NM_138509.3 , XM_006235294.3
Accession Version	XM_006235294.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	microtubule-associated protein RP/EB family member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006235294.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGTGA ATGTGTACTC GACGTCAGTC ACCAGTGATA ACCTCAGTCG ACATGACATG 
CTGGCTTGGA TCAATGAATC TCTGCAGTTG AATCTGACAA AGATAGAACA GCTATGTTCA 
GGGGCTGCAT ACTGTCAGTT CATGGACATG CTCTTCCCCG GCTCCATTGC CTTGAAGAAA 
GTGAAATTCC AGGCTAAGCT AGAACATGAA TATATCCAGA ACTTCAAAAT ACTACAAGCC 
GGCTTCAAGA GGATGGGCGT TGACAAAATA ATTCCTGTGG ATAAATTAGT AAAAGGAAAA 
TTTCAGGACA ATTTTGAATT CGTTCAATGG TTCAAGAAGT TTTTTGATGC AAATTATGAT 
GGAAAAGAAT ATGATCCTGT CGCTGCCAGA CAAGGTCAAG AAACGGCAGT GGCTCCTTCT 
CTTGTCGCTC CAGCTTTGAG TAAACCGAAG AAACCTCTCG GCTCCGGTAG TGCAGCCCCA 
CAGAGACCCA TTGCAACACA GAGGACTACT GCAGCTCCTA AGGCTGGCCC CGGGATGGTG 
CGAAAGAATC CTGGCATGGG CAATGGAGAT GACGAAGCTG CTGAATTGAT GCAGCAGGTC 
AAAGTATTGA AGCTTACTGT TGAAGACTTG GAGAAGGAGA GAGATTTCTA CTTCGGCAAG 
CTAAGGAACA TTGAATTGAT TTGCCAGGAG AATGAGGGGG AAAATGACCC TGTACTGCAG 
AGGATTGTAG ATATTCTTTA TGCCACAGAT GAAGGCTTTG TGATACCTGA CGAGGGGGGC 
CCACAGGAGG AACAAGAAGA GTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006235356.1
CDS	83..889
Translation	MAVNVYSTSVTSDNLSRHDMLAWINESLQLNLTKIEQLCSGAAYCQFMDMLFPGSIALKKVKFQAKLEHEYIQNFKILQAGFKRMGVDKIIPVDKLVKGKFQDNFEFVQWFKKFFDANYDGKEYDPVAARQGQETAVAPSLVAPALSKPKKPLGSGSAAPQRPIATQRTTAAPKAGPGMVRKNPGMGNGDDEAAELMQQVKVLKLTVEDLEKERDFYFGKLRNIELICQENEGENDPVLQRIVDILYATDEGFVIPDEGGPQEEQEEY

Target ORF information:

RefSeq Version	XM_006235294.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus microtubule-associated protein, RP/EB family, member 1 (Mapre1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006235294.3

ORF Insert Sequence:

ATGGCAGTGA ATGTGTACTC GACGTCAGTC ACCAGTGATA ACCTCAGTCG ACATGACATG 
CTGGCTTGGA TCAATGAATC TCTGCAGTTG AATCTGACAA AGATAGAACA GCTATGTTCA 
GGGGCTGCAT ACTGTCAGTT CATGGACATG CTCTTCCCCG GCTCCATTGC CTTGAAGAAA 
GTGAAATTCC AGGCTAAGCT AGAACATGAA TATATCCAGA ACTTCAAAAT ACTACAAGCC 
GGCTTCAAGA GGATGGGCGT TGACAAAATA ATTCCTGTGG ATAAATTAGT AAAAGGAAAA 
TTTCAGGACA ATTTTGAATT CGTTCAATGG TTCAAGAAGT TTTTTGATGC AAATTATGAT 
GGAAAAGAAT ATGATCCTGT CGCTGCCAGA CAAGGTCAAG AAACGGCAGT GGCTCCTTCT 
CTTGTCGCTC CAGCTTTGAG TAAACCGAAG AAACCTCTCG GCTCCGGTAG TGCAGCCCCA 
CAGAGACCCA TTGCAACACA GAGGACTACT GCAGCTCCTA AGGCTGGCCC CGGGATGGTG 
CGAAAGAATC CTGGCATGGG CAATGGAGAT GACGAAGCTG CTGAATTGAT GCAGCAGGTC 
AAAGTATTGA AGCTTACTGT TGAAGACTTG GAGAAGGAGA GAGATTTCTA CTTCGGCAAG 
CTAAGGAACA TTGAATTGAT TTGCCAGGAG AATGAGGGGG AAAATGACCC TGTACTGCAG 
AGGATTGTAG ATATTCTTTA TGCCACAGAT GAAGGCTTTG TGATACCTGA CGAGGGGGGC 
CCACAGGAGG AACAAGAAGA GTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

EB1-binding-myomegalin protein complex promotes centrosomal microtubules functions.
Proceedings of the National Academy of Sciences of the United States of America114(50)E10687-E10696(2017 Dec)
Bouguenina H,Salaun D,Mangon A,Muller L,Baudelet E,Camoin L,Tachibana T,Cianf?rani S,Audebert S,Verdier-Pinard P,Badache A

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Molecular systems biology12(4)863(2016 Apr)
Yachie N,Petsalaki E,Mellor JC,Weile J,Jacob Y,Verby M,Ozturk SB,Li S,Cote AG,Mosca R,Knapp JJ,Ko M,Yu A,Gebbia M,Sahni N,Yi S,Tyagi T,Sheykhkarimli D,Roth JF,Wong C,Musa L,Snider J,Liu YC,Yu H,Braun P,Stagljar I,Hao T,Calderwood MA,Pelletier L,Aloy P,Hill DE,Vidal M,Roth FP

Signaling Scaffold Protein IQGAP1 Interacts with Microtubule Plus-end Tracking Protein SKAP and Links Dynamic Microtubule Plus-end to Steer Cell Migration.
The Journal of biological chemistry290(39)23766-80(2015 Sep)
Cao D,Su Z,Wang W,Wu H,Liu X,Akram S,Qin B,Zhou J,Zhuang X,Adams G,Jin C,Wang X,Liu L,Hill DL,Wang D,Ding X,Yao X

TTBK2 with EB1/3 regulates microtubule dynamics in migrating cells through KIF2A phosphorylation.
The Journal of cell biology210(5)737-51(2015 Aug)
Watanabe T,Kakeno M,Matsui T,Sugiyama I,Arimura N,Matsuzawa K,Shirahige A,Ishidate F,Nishioka T,Taya S,Hoshino M,Kaibuchi K

Microtubules regulate focal adhesion dynamics through MAP4K4.
Developmental cell31(5)572-85(2014 Dec)
Yue J,Xie M,Gou X,Lee P,Schneider MD,Wu X

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Mapre1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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