Rgs14 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rgs14
Entrez Gene ID	114705
Full Name	regulator of G-protein signaling 14
General protein information	Preferred Names regulator of G-protein signaling 14 Names regulator of G-protein signaling 14
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 17 Map Location: 17p14
Summary	binds Galpha(i1)GDP and acts as a GTPase activator by maintaining G-protein subunit dissociation; regulates G-protein mediated signaling [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053764.1	NP_446216.1	regulator of G-protein signaling 14

Pathways from BioSystems

Homologies

	RGS14	XP_001089197.2
Pan troglodytes (chimpanzee)	RGS14	XP_001142206.2
Canis lupus familiaris (dog)	RGS14	XP_005619218.1
Mus musculus (house mouse)	Rgs14	NP_058038.2
Homo sapiens (human)	RGS14	NP_006471.2
Bos taurus (cattle)	RGS14	NP_001179660.1
Danio rerio (zebrafish)	rgs14a	NP_958461.1
Rattus norvegicus (Norway rat)	Rgs14	NP_446216.1
Gallus gallus (chicken)	RGS14	XP_001231918.3
Danio rerio (zebrafish)	LOC100003648	XP_001343150.3
Xenopus tropicalis (tropical clawed frog)	LOC100490892	XP_002938108.2

Gene Ontology
Bibliography

Related articles in PubMed

Regulator of G protein signaling 14 (RGS14) is expressed pre- and postsynaptically in neurons of hippocampus, basal ganglia, and amygdala of monkey and human brain.
Squires KE, Gerber KJ, Pare JF, Branch MR, Smith Y, Hepler JR
Brain structure & function223(1)233-253(2018 Jan)

Endogenous RGS14 is a cytoplasmic-nuclear shuttling protein that localizes to juxtanuclear membranes and chromatin-rich regions of the nucleus.
Branch MR, Hepler JR
PloS one12(9)e0184497(2017)

Assembly and function of the regulator of G protein signaling 14 (RGS14)?H-Ras signaling complex in live cells are regulated by G?i1 and G?i-linked G protein-coupled receptors.
Vellano CP, Brown NE, Blumer JB, Hepler JR
The Journal of biological chemistry288(5)3620-31(2013 Feb)

A genome-wide association study of nephrolithiasis in the Japanese population identifies novel susceptible Loci at 5q35.3, 7p14.3, and 13q14.1.
Urabe Y, Tanikawa C, Takahashi A, Okada Y, Morizono T, Tsunoda T, Kamatani N, Kohri K, Chayama K, Kubo M, Nakamura Y, Matsuda K
PLoS genetics8(3)e1002541(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results show that endogenous RGS14 localizes to subcellular compartments not previously recognized in studies of recombinant RGS14 in unnatural host cells. The two most surprising observations were that RGS14 is not enriched at the plasma membrane, and that it is found within chromatin-rich regions of the nucleus in close proximity with active RNA Pol-II.
Title: Endogenous RGS14 is a cytoplasmic-nuclear shuttling protein that localizes to juxtanuclear membranes and chromatin-rich regions of the nucleus.

Inactive Galpha(i1)-GDP enhances the affinity of RGS14 for H-Ras-GTP in live cells, resulting in a ternary signaling complex that is further regulated by G protein-coupled receptors.
Title: Assembly and function of the regulator of G protein signaling 14 (RGS14)?H-Ras signaling complex in live cells are regulated by G?i1 and G?i-linked G protein-coupled receptors.

RGS14 can form complexes with GPCRs in cells that are dependent on Galpha(i/o) and these RGS14.Galpha(i1).GPCR complexes may be substrates for other signaling partners such as Ric-8A
Title: G protein-coupled receptors and resistance to inhibitors of cholinesterase-8A (Ric-8A) both regulate the regulator of g protein signaling 14 RGS14?G?i1 complex in live cells.

RGS proteins with with GTPase-activating protein (GAP) activity affect both stress resistance and longevity in several species.
Title: Regulation of longevity by regulator of G-protein signaling protein, Loco.

RGS14 facilitates the formation of a selective Ras.GTP-Raf-MEK-ERK multiprotein complex to promote sustained ERK activation and regulate H-Ras-dependent neuritogenesis.
Title: Regulator of G-protein signaling 14 (RGS14) is a selective H-Ras effector.

The following Rgs14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rgs14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10555	NM_053764.1 Latest version!	Rattus norvegicus regulator of G-protein signaling 14 (Rgs14), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rgs14 ( NM_053764.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446216.1 Rattus norvegicus regulator of G-protein signaling 14 (Rgs14), mRNA.

Price & Availability↑

CloneID	ORa10555
Clone ID Related Accession (Same CDS sequence)	NM_053764.1
Accession Version	NM_053764.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	regulator of G-protein signaling 14
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U92279.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U92279.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMEA2689594 [ECO:0000350] ##Evidence-Data-END##

ATGCCAGGGA AGCCCAAGCA CTTGGGTGTC CCCAACGGGC GCATGGTCCT GGCTGTCTCT 
GATGGAGAAC TGACCAGCAC ATCAGGTTCC CAGGCCCAGG GTGAGGGCAG AGGCAGCTCC 
CTCAGCATCC ACAGCCTCCC CAGTGGCCCC AGCAGCCCCT TCTCCACTGA TGAGCAGCCT 
GTGGCCAGCT GGGCCCAGTC CTTTGAGCGG CTGCTGCAAG ACCCACGGGG TCTGGCTTAC 
TTCACTGAGT TCCTGAAGAA GGAATTCAGC GCCGAGAATG TAACTTTCTG GCAAGCCTGC 
GAGCGTTTCC AACAGATCCC AGCCAGCGAC ACCAAACAGC TAGCTCAGGA GGCCCACAAC 
ATCTACCATG AGTTCCTGTC CAGCCAGGCA CTGAGCCCAG TGAACATCGA CCGACAGGCC 
TGGCTTAGTG AGGAGGTGCT GGCCCAGCCC AGGCCCGATA TGTTCCGAGC ACAGCAGCTT 
CAGATCTTCA ATTTGATGAA GTTCGACAGC TACGCGCGCT TCGTCAAATC CCCGCTGTAC 
CAAGAGTGCC TGCTGGCCGA GGCCGAGGGA CGCCCCCTGC GGGAACCTGG ATCCTCACAC 
CTCGGGAGCC CGGACACTGC GAGGAAGAAG CCAAAGCTGA AGCCTGGAAA GTCACTGCCG 
CTCGGTGTGG AAGAGTTGGG GCAGCTGCCG CTTGCTGAGG GCCGCCCTCT CCGCAAGTCC 
TTTCGTAGAG AGATGCCGGG TGGAGCTGTG AATTCGGCCC TGCGACGAGA GTCTCAGGGA 
TCCCTGAATT CTTCCGCGAG TCTGGACCTG GGCTTCCTTG CCTTTGTGAG CAGCAAATCC 
GAGAGCCACC GAAAGAGCCT TGGAAGTGGA GAGGGTGAGA GCGAAAGCCG GCCCGGGAAG 
TACTGCTGCG TGTATCTACC TGATGGCACG GCCTCCTTGG CCCTGGCTCG ACCTGGCCTC 
ACCATCCGTG ACATGCTGGC AGGCATCTGT GAGAAGAGAG GCCTCTCTCT ACCTGACATT 
AAGGTCTACC TGGTGGGCAA AGAACAGAAG GCCCTGGTCC TGGATCAGGA CTGCACCGTG 
CTGGCAGACC AGGAAGTGCG ACTGGAAAAC AGGATCACCT TCCAGCTGGA GTTGGTCGGC 
CTGGAGCGTG TGGTACGGAT CTCTGCTAAG CCCACCAAGC GTCTGCAGGA GGCGCTGCAG 
CCTATCTTGG CCAAGCACGG CCTGAGCCTG GATCAGGTGG TCCTACACAG GCCAGGAGAG 
AAGCAGCTAG TGGATTTGGA GAATCTAGTG AGCTCAGTGG CTTCACAGAC ACTGGTTTTG 
GACACTCTTC CAGATGCAAA GACAAGGGAA GCCAGCAGCA TACCTCCCTG CCGCAGCCAG 
GGATGCCTCC CTAGAACCCA GACCAAGGAC AGTCACCTTC CTCCACTGTC CTCCAGTTTG 
TCGGTAGAAG ATGCCAGTGG TTCTACTGGG AAACGACAGA CCTGCGACAT TGAAGGCCTA 
GTGGAGCTGC TGAATCGTGT GCAGAGCAGC GGGGCCCATG ACCAGAGGGG ACTTCTTCGC 
AAAGAGGACC TGGTCCTTCC AGAATTTCTG CAGCTTCCTT CCCAAAGACC AGGTTCTCAG 
GAGGCTCCAC CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446216.1
CDS	265..1899
Translation	MPGKPKHLGVPNGRMVLAVSDGELTSTSGSQAQGEGRGSSLSIHSLPSGPSSPFSTDEQPVASWAQSFERLLQDPRGLAYFTEFLKKEFSAENVTFWQACERFQQIPASDTKQLAQEAHNIYHEFLSSQALSPVNIDRQAWLSEEVLAQPRPDMFRAQQLQIFNLMKFDSYARFVKSPLYQECLLAEAEGRPLREPGSSHLGSPDTARKKPKLKPGKSLPLGVEELGQLPLAEGRPLRKSFRREMPGGAVNSALRRESQGSLNSSASLDLGFLAFVSSKSESHRKSLGSGEGESESRPGKYCCVYLPDGTASLALARPGLTIRDMLAGICEKRGLSLPDIKVYLVGKEQKALVLDQDCTVLADQEVRLENRITFQLELVGLERVVRISAKPTKRLQEALQPILAKHGLSLDQVVLHRPGEKQLVDLENLVSSVASQTLVLDTLPDAKTREASSIPPCRSQGCLPRTQTKDSHLPPLSSSLSVEDASGSTGKRQTCDIEGLVELLNRVQSSGAHDQRGLLRKEDLVLPEFLQLPSQRPGSQEAPP

Target ORF information:

RefSeq Version	NM_053764.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulator of G-protein signaling 14 (Rgs14), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053764.1

ORF Insert Sequence:

ATGCCAGGGA AGCCCAAGCA CTTGGGTGTC CCCAACGGGC GCATGGTCCT GGCTGTCTCT 
GATGGAGAAC TGACCAGCAC ATCAGGTTCC CAGGCCCAGG GTGAGGGCAG AGGCAGCTCC 
CTCAGCATCC ACAGCCTCCC CAGTGGCCCC AGCAGCCCCT TCTCCACTGA TGAGCAGCCT 
GTGGCCAGCT GGGCCCAGTC CTTTGAGCGG CTGCTGCAAG ACCCACGGGG TCTGGCTTAC 
TTCACTGAGT TCCTGAAGAA GGAATTCAGC GCCGAGAATG TAACTTTCTG GCAAGCCTGC 
GAGCGTTTCC AACAGATCCC AGCCAGCGAC ACCAAACAGC TAGCTCAGGA GGCCCACAAC 
ATCTACCATG AGTTCCTGTC CAGCCAGGCA CTGAGCCCAG TGAACATCGA CCGACAGGCC 
TGGCTTAGTG AGGAGGTGCT GGCCCAGCCC AGGCCCGATA TGTTCCGAGC ACAGCAGCTT 
CAGATCTTCA ATTTGATGAA GTTCGACAGC TACGCGCGCT TCGTCAAATC CCCGCTGTAC 
CAAGAGTGCC TGCTGGCCGA GGCCGAGGGA CGCCCCCTGC GGGAACCTGG ATCCTCACAC 
CTCGGGAGCC CGGACACTGC GAGGAAGAAG CCAAAGCTGA AGCCTGGAAA GTCACTGCCG 
CTCGGTGTGG AAGAGTTGGG GCAGCTGCCG CTTGCTGAGG GCCGCCCTCT CCGCAAGTCC 
TTTCGTAGAG AGATGCCGGG TGGAGCTGTG AATTCGGCCC TGCGACGAGA GTCTCAGGGA 
TCCCTGAATT CTTCCGCGAG TCTGGACCTG GGCTTCCTTG CCTTTGTGAG CAGCAAATCC 
GAGAGCCACC GAAAGAGCCT TGGAAGTGGA GAGGGTGAGA GCGAAAGCCG GCCCGGGAAG 
TACTGCTGCG TGTATCTACC TGATGGCACG GCCTCCTTGG CCCTGGCTCG ACCTGGCCTC 
ACCATCCGTG ACATGCTGGC AGGCATCTGT GAGAAGAGAG GCCTCTCTCT ACCTGACATT 
AAGGTCTACC TGGTGGGCAA AGAACAGAAG GCCCTGGTCC TGGATCAGGA CTGCACCGTG 
CTGGCAGACC AGGAAGTGCG ACTGGAAAAC AGGATCACCT TCCAGCTGGA GTTGGTCGGC 
CTGGAGCGTG TGGTACGGAT CTCTGCTAAG CCCACCAAGC GTCTGCAGGA GGCGCTGCAG 
CCTATCTTGG CCAAGCACGG CCTGAGCCTG GATCAGGTGG TCCTACACAG GCCAGGAGAG 
AAGCAGCTAG TGGATTTGGA GAATCTAGTG AGCTCAGTGG CTTCACAGAC ACTGGTTTTG 
GACACTCTTC CAGATGCAAA GACAAGGGAA GCCAGCAGCA TACCTCCCTG CCGCAGCCAG 
GGATGCCTCC CTAGAACCCA GACCAAGGAC AGTCACCTTC CTCCACTGTC CTCCAGTTTG 
TCGGTAGAAG ATGCCAGTGG TTCTACTGGG AAACGACAGA CCTGCGACAT TGAAGGCCTA 
GTGGAGCTGC TGAATCGTGT GCAGAGCAGC GGGGCCCATG ACCAGAGGGG ACTTCTTCGC 
AAAGAGGACC TGGTCCTTCC AGAATTTCTG CAGCTTCCTT CCCAAAGACC AGGTTCTCAG 
GAGGCTCCAC CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulator of G protein signaling 14 (RGS14) is expressed pre- and postsynaptically in neurons of hippocampus, basal ganglia, and amygdala of monkey and human brain.
Brain structure & function223(1)233-253(2018 Jan)
Squires KE,Gerber KJ,Pare JF,Branch MR,Smith Y,Hepler JR

Endogenous RGS14 is a cytoplasmic-nuclear shuttling protein that localizes to juxtanuclear membranes and chromatin-rich regions of the nucleus.
PloS one12(9)e0184497(2017)
Branch MR,Hepler JR

Assembly and function of the regulator of G protein signaling 14 (RGS14)?H-Ras signaling complex in live cells are regulated by G?i1 and G?i-linked G protein-coupled receptors.
The Journal of biological chemistry288(5)3620-31(2013 Feb)
Vellano CP,Brown NE,Blumer JB,Hepler JR

A genome-wide association study of nephrolithiasis in the Japanese population identifies novel susceptible Loci at 5q35.3, 7p14.3, and 13q14.1.
PLoS genetics8(3)e1002541(2012)
Urabe Y,Tanikawa C,Takahashi A,Okada Y,Morizono T,Tsunoda T,Kamatani N,Kohri K,Chayama K,Kubo M,Nakamura Y,Matsuda K

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Rgs14 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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