Dmrt1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dmrt1
Entrez Gene ID	114498
Full Name	doublesex and mab-3 related transcription factor 1
General protein information	Preferred Names doublesex and mab-3 related transcription factor 1 Names doublesex- and mab-3-related transcription factor 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q51
Summary	transcription factor required for postnatal testis differentiation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053706.1	NP_446158.1	doublesex- and mab-3-related transcription factor 1
XM_008760301.1	XP_008758523.1	doublesex- and mab-3-related transcription factor 1 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DMRT1	NP_001071528.1
Homo sapiens (human)	DMRT1	NP_068770.2
Canis lupus familiaris (dog)	DMRT1	XP_851495.1
Rattus norvegicus (Norway rat)	Dmrt1	NP_446158.1
Danio rerio (zebrafish)	dmrt1	NP_991191.1
	LOC100425397	XP_002800204.1
Pan troglodytes (chimpanzee)	DMRT1	XP_003312067.1
Xenopus tropicalis (tropical clawed frog)	dmrt1	XP_002935648.2
Mus musculus (house mouse)	Dmrt1	NP_056641.2

Gene Ontology
Bibliography

Related articles in PubMed

An ancient protein-DNA interaction underlying metazoan sex determination.
Murphy MW, Lee JK, Rojo S, Gearhart MD, Kurahashi K, Banerjee S, Loeuille GA, Bashamboo A, McElreavey K, Zarkower D, Aihara H, Bardwell VJ
Nature structural & molecular biology22(6)442-51(2015 Jun)

Interaction between DMRT1 function and genetic background modulates signaling and pluripotency to control tumor susceptibility in the fetal germ line.
Krentz AD, Murphy MW, Zhang T, Sarver AL, Jain S, Griswold MD, Bardwell VJ, Zarkower D
Developmental biology377(1)67-78(2013 May)

Analysis of meiosis regulators in human gonads: a sexually dimorphic spatio-temporal expression pattern suggests involvement of DMRT1 in meiotic entry.
J?rgensen A, Nielsen JE, Blomberg Jensen M, Gr?m N, Rajpert-De Meyts E
Molecular human reproduction18(11)523-34(2012 Nov)

DMRT1 promotes oogenesis by transcriptional activation of Stra8 in the mammalian fetal ovary.
Krentz AD, Murphy MW, Sarver AL, Griswold MD, Bardwell VJ, Zarkower D
Developmental biology356(1)63-70(2011 Aug)

DMRT1 prevents female reprogramming in the postnatal mammalian testis.
Matson CK, Murphy MW, Sarver AL, Griswold MD, Bardwell VJ, Zarkower D
Nature476(7358)101-4(2011 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

FOXL2, a transcription factor required for differentiation of the ovary, repressed Dmrt1 promoter through the -3.2 kb/-2.8 kb regulatory region, offering a potential mechanism for Dmrt1 transcriptional silencing in granulosa cells.
Title: Distinct transcriptional mechanisms direct expression of the rat Dmrt1 promoter in sertoli cells and germ cells of transgenic mice.

Sp1, Sp3, and Egr1 transcription factors play a critical role in Dmrt1 regulation in the testis
Title: Sp1 and Egr1 regulate transcription of the Dmrt1 gene in Sertoli cells.

The following Dmrt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dmrt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10368	NM_053706.1 Latest version!	Rattus norvegicus doublesex and mab-3 related transcription factor 1 (Dmrt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa43933	XM_008760301.1 Latest version!	Rattus norvegicus doublesex and mab-3 related transcription factor 1 (Dmrt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dmrt1 ( NM_053706.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446158.1 Rattus norvegicus doublesex and mab-3 related transcription factor 1 (Dmrt1), mRNA.

Price & Availability↑

CloneID	ORa10368
Clone ID Related Accession (Same CDS sequence)	NM_053706.1
Accession Version	NM_053706.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-26
Organism	Rattus norvegicus(Norway rat)
Product	doublesex- and mab-3-related transcription factor 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF379608.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF379608.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2689604 [ECO:0000350] ##Evidence-Data-END##

ATGCCTAACG ACGACGCATT CGGCAAGCCC TCTGCACCGA CGGAGGTTCC TCACGCTCCC 
GGGGCTCCGC CGCAGGGCAA AGCCGGGGGC TACAGCAAAG CCACTGGGGC GATGGCCGGA 
GCAGCCGGGG GCTCCAGTGC GGGGGGCAGC GGAGGTGCCT CGGGCTCCGG GCCGTCGGGC 
CTGGGCTCTG GAAGCAAGAA GTCCCCGAGG CTGCCCAAAT GCGCTCGCTG CAGGAACCAC 
GGCTACGCGT CGCCGCTCAA GGGCCACAAG CGCTTTTGCA TGTGGCGGGA CTGCCAGTGC 
AAGAAGTGCA GCCTGATTGC CGAGCGACAG CGGGTGATGG CCGCGCAGGT GGCCCTGAGA 
AGACAGCAGG CCCAGGAAGA GGAGCTGGGC ATCAGCCACC CCATTCCGCT GCCCAGCGCA 
GCAGAGCTGC TGGTCAAAAG AGAGAATAGT GCCAGCAACC CGTGCCTGAT GGCTGAGAGC 
AGCAGCTCGG CCCAGCCCCC ACCGGCCAGC ACCCCAACCC CCACTGTCTC AGAGGGACGC 
ATGGTCATCC AGGATATCCC TGCTGTCACC AGCAGAGGGC ACATGGAGAA CGCACCTGAC 
CTGATGTCAG ACCCCGCCTA CTACAGCAGC TTTTACCAGC CGTCTCTGTT TCCTTACTAC 
AACAATCTGT ACAACTACCC CCAGTACTCC ATGGCCTTGT CTGCTGAGTC CTCCTCAGGG 
GACGTGGGAA ATCCCCTTGG GGGATCCCCT GTGAAGAACA GCCTGCGTAG TCTCCCAGCA 
CCTTATGTGC CTGCTCAGAC TGGAAACCAG TGGCAGATGA AGACCTCAGA GGGCCGCCAC 
CCAGTGAGCT CCCAGTACCG GATGCACTCA TACTATGGGC CTCCCTCCTA CCTGGGTCAG 
AGCATGTCCC AGATCTTTAC CTTTGAGGAA GGCCCCTCCT ACTCAGAAGC CAAAGCAAGT 
GTGTTCTCGC CGCCCAGCAG TCAAGATTCT GGCTTGGTTT CCCTCTCCAG CAGCTCTCCG 
ATGAGCAACG AGAGCACGAA GGGAGTTCTG GAATGCGAGT CTGCGTCCTC TGAACCTAGC 
AGCTACGCGG TCAACCAGGT CCTGGAAGAG GATGAGGACG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446158.1
CDS	103..1227
Translation	MPNDDAFGKPSAPTEVPHAPGAPPQGKAGGYSKATGAMAGAAGGSSAGGSGGASGSGPSGLGSGSKKSPRLPKCARCRNHGYASPLKGHKRFCMWRDCQCKKCSLIAERQRVMAAQVALRRQQAQEEELGISHPIPLPSAAELLVKRENSASNPCLMAESSSSAQPPPASTPTPTVSEGRMVIQDIPAVTSRGHMENAPDLMSDPAYYSSFYQPSLFPYYNNLYNYPQYSMALSAESSSGDVGNPLGGSPVKNSLRSLPAPYVPAQTGNQWQMKTSEGRHPVSSQYRMHSYYGPPSYLGQSMSQIFTFEEGPSYSEAKASVFSPPSSQDSGLVSLSSSSPMSNESTKGVLECESASSEPSSYAVNQVLEEDEDE

Target ORF information:

RefSeq Version	NM_053706.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus doublesex and mab-3 related transcription factor 1 (Dmrt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053706.1

ORF Insert Sequence:

ATGCCTAACG ACGACGCATT CGGCAAGCCC TCTGCACCGA CGGAGGTTCC TCACGCTCCC 
GGGGCTCCGC CGCAGGGCAA AGCCGGGGGC TACAGCAAAG CCACTGGGGC GATGGCCGGA 
GCAGCCGGGG GCTCCAGTGC GGGGGGCAGC GGAGGTGCCT CGGGCTCCGG GCCGTCGGGC 
CTGGGCTCTG GAAGCAAGAA GTCCCCGAGG CTGCCCAAAT GCGCTCGCTG CAGGAACCAC 
GGCTACGCGT CGCCGCTCAA GGGCCACAAG CGCTTTTGCA TGTGGCGGGA CTGCCAGTGC 
AAGAAGTGCA GCCTGATTGC CGAGCGACAG CGGGTGATGG CCGCGCAGGT GGCCCTGAGA 
AGACAGCAGG CCCAGGAAGA GGAGCTGGGC ATCAGCCACC CCATTCCGCT GCCCAGCGCA 
GCAGAGCTGC TGGTCAAAAG AGAGAATAGT GCCAGCAACC CGTGCCTGAT GGCTGAGAGC 
AGCAGCTCGG CCCAGCCCCC ACCGGCCAGC ACCCCAACCC CCACTGTCTC AGAGGGACGC 
ATGGTCATCC AGGATATCCC TGCTGTCACC AGCAGAGGGC ACATGGAGAA CGCACCTGAC 
CTGATGTCAG ACCCCGCCTA CTACAGCAGC TTTTACCAGC CGTCTCTGTT TCCTTACTAC 
AACAATCTGT ACAACTACCC CCAGTACTCC ATGGCCTTGT CTGCTGAGTC CTCCTCAGGG 
GACGTGGGAA ATCCCCTTGG GGGATCCCCT GTGAAGAACA GCCTGCGTAG TCTCCCAGCA 
CCTTATGTGC CTGCTCAGAC TGGAAACCAG TGGCAGATGA AGACCTCAGA GGGCCGCCAC 
CCAGTGAGCT CCCAGTACCG GATGCACTCA TACTATGGGC CTCCCTCCTA CCTGGGTCAG 
AGCATGTCCC AGATCTTTAC CTTTGAGGAA GGCCCCTCCT ACTCAGAAGC CAAAGCAAGT 
GTGTTCTCGC CGCCCAGCAG TCAAGATTCT GGCTTGGTTT CCCTCTCCAG CAGCTCTCCG 
ATGAGCAACG AGAGCACGAA GGGAGTTCTG GAATGCGAGT CTGCGTCCTC TGAACCTAGC 
AGCTACGCGG TCAACCAGGT CCTGGAAGAG GATGAGGACG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dmrt1 ( XM_008760301.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758523.1 Rattus norvegicus doublesex and mab-3 related transcription factor 1 (Dmrt1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa43933
Clone ID Related Accession (Same CDS sequence)	XM_008760301.1
Accession Version	XM_008760301.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	doublesex- and mab-3-related transcription factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTAACG ACGACGCATT CGGCAAGCCC TCTGCACCGA CGGAGGTTCC TCACGCTCCC 
GGGGCTCCGC CGCAGGGCAA AGCCGGGGGC TACAGCAAAG CCACTGGAGC GATGGCCGGA 
GCAGCCGGGG GCTCCAGTGC GGGGGGCAGC GGAGGTGCCT CGGGCTCCGG GCCGTCGGGC 
CTGGGCTCTG GAAGCAAGAA GTCCCCGAGG CTGCCCAAAT GCGCTCGCTG CAGGAACCAC 
GGCTACGCGT CGCCGCTCAA GGGCCACAAG CGCTTTTGCA TGTGGCGGGA CTGCCAGTGC 
AAGAAGTGCA GCCTGATTGC CGAGCGACAG CGGGTGATGG CCGCGCAGGT GGCCCTGAGA 
AGACAGCAGG CCCAGGAAGA GGAGCTGGGC ATCAGCCACC CCATTCCGCT GCCCAGCGCA 
GCAGAGCTGC TGGTCAAAAG AGAGAATAGT GCCAGCAACC CGTGCCTGAT GGCTGAGAGC 
AGCAGCTCGG CCCAGCCCCC ACCGGCCAGC ACCCCAACCC CCACTGTCTC AGAGGGACGC 
ATGGTCATCC AGGATATCCC TGCTGTCACC AGCAGAGGGC ACATGGAGAA CGCACCTGAC 
CTGATGTCAG ACCCCGCCTA CTACAGCAGC TTTTACCAGC CGTCTCTGTT TCCTTACTAC 
AACAATCTGT ACAACTACCC CCAGTACTCC ATGGCCTTGT CTGCTGAGTC CTCCTCAGGG 
GACGTGGGAA ATCCCCTTGG GGGATCCCCT GTGAAGAACA GCCTGCGTAG TCTCCCAGCA 
CCTTATGTGC CTGCTCAGAC TGGAAACCAG TGGCAGATGA AGACCTCAGA GGGCCGCCAC 
CCAGTGAGCT CCCAGTACCG GATGCACTCA TACTATGGGC CTCCCTCCTA CCTGGGTCAG 
AGCATGTCCC AGATCTTTAC CTTTGAGGAA GGCCCCTCCT ACTCAGAAGC CAAAGCAAGT 
GGTAAGTTCT GTGGTGGGAA TCTGGGATTG GTACCCAGCC GGGAGCGAGC GGTGACTCTC 
TGTGGGCTTG GCTCACAGGA GCTTTGGGGG AATTGCTTTT TTAATTCTTT GAGACTGGCA 
TACAACTCTT TCCACCTTCA TATCTACCCA ACGTCTTCCT CCTTCTCCCT CTTCCCCTGT 
CCTGTGGAGT ACAGTTTATG CTATGACTTG CCCTGGAGTA TGGTTAATAA AGTTGGTATC 
ACATTTTTAA TGAAAGCTTA CTTTCCTTCT CCAAGCAACA ATCAAATACC AACAGCTTCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758523.1
CDS	193..1455
Translation	MPNDDAFGKPSAPTEVPHAPGAPPQGKAGGYSKATGAMAGAAGGSSAGGSGGASGSGPSGLGSGSKKSPRLPKCARCRNHGYASPLKGHKRFCMWRDCQCKKCSLIAERQRVMAAQVALRRQQAQEEELGISHPIPLPSAAELLVKRENSASNPCLMAESSSSAQPPPASTPTPTVSEGRMVIQDIPAVTSRGHMENAPDLMSDPAYYSSFYQPSLFPYYNNLYNYPQYSMALSAESSSGDVGNPLGGSPVKNSLRSLPAPYVPAQTGNQWQMKTSEGRHPVSSQYRMHSYYGPPSYLGQSMSQIFTFEEGPSYSEAKASGKFCGGNLGLVPSRERAVTLCGLGSQELWGNCFFNSLRLAYNSFHLHIYPTSSSFSLFPCPVEYSLCYDLPWSMVNKVGITFLMKAYFPSPSNNQIPTAS

Target ORF information:

RefSeq Version	XM_008760301.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus doublesex and mab-3 related transcription factor 1 (Dmrt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760301.1

ORF Insert Sequence:

ATGCCTAACG ACGACGCATT CGGCAAGCCC TCTGCACCGA CGGAGGTTCC TCACGCTCCC 
GGGGCTCCGC CGCAGGGCAA AGCCGGGGGC TACAGCAAAG CCACTGGAGC GATGGCCGGA 
GCAGCCGGGG GCTCCAGTGC GGGGGGCAGC GGAGGTGCCT CGGGCTCCGG GCCGTCGGGC 
CTGGGCTCTG GAAGCAAGAA GTCCCCGAGG CTGCCCAAAT GCGCTCGCTG CAGGAACCAC 
GGCTACGCGT CGCCGCTCAA GGGCCACAAG CGCTTTTGCA TGTGGCGGGA CTGCCAGTGC 
AAGAAGTGCA GCCTGATTGC CGAGCGACAG CGGGTGATGG CCGCGCAGGT GGCCCTGAGA 
AGACAGCAGG CCCAGGAAGA GGAGCTGGGC ATCAGCCACC CCATTCCGCT GCCCAGCGCA 
GCAGAGCTGC TGGTCAAAAG AGAGAATAGT GCCAGCAACC CGTGCCTGAT GGCTGAGAGC 
AGCAGCTCGG CCCAGCCCCC ACCGGCCAGC ACCCCAACCC CCACTGTCTC AGAGGGACGC 
ATGGTCATCC AGGATATCCC TGCTGTCACC AGCAGAGGGC ACATGGAGAA CGCACCTGAC 
CTGATGTCAG ACCCCGCCTA CTACAGCAGC TTTTACCAGC CGTCTCTGTT TCCTTACTAC 
AACAATCTGT ACAACTACCC CCAGTACTCC ATGGCCTTGT CTGCTGAGTC CTCCTCAGGG 
GACGTGGGAA ATCCCCTTGG GGGATCCCCT GTGAAGAACA GCCTGCGTAG TCTCCCAGCA 
CCTTATGTGC CTGCTCAGAC TGGAAACCAG TGGCAGATGA AGACCTCAGA GGGCCGCCAC 
CCAGTGAGCT CCCAGTACCG GATGCACTCA TACTATGGGC CTCCCTCCTA CCTGGGTCAG 
AGCATGTCCC AGATCTTTAC CTTTGAGGAA GGCCCCTCCT ACTCAGAAGC CAAAGCAAGT 
GGTAAGTTCT GTGGTGGGAA TCTGGGATTG GTACCCAGCC GGGAGCGAGC GGTGACTCTC 
TGTGGGCTTG GCTCACAGGA GCTTTGGGGG AATTGCTTTT TTAATTCTTT GAGACTGGCA 
TACAACTCTT TCCACCTTCA TATCTACCCA ACGTCTTCCT CCTTCTCCCT CTTCCCCTGT 
CCTGTGGAGT ACAGTTTATG CTATGACTTG CCCTGGAGTA TGGTTAATAA AGTTGGTATC 
ACATTTTTAA TGAAAGCTTA CTTTCCTTCT CCAAGCAACA ATCAAATACC AACAGCTTCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An ancient protein-DNA interaction underlying metazoan sex determination.
Nature structural & molecular biology22(6)442-51(2015 Jun)
Murphy MW,Lee JK,Rojo S,Gearhart MD,Kurahashi K,Banerjee S,Loeuille GA,Bashamboo A,McElreavey K,Zarkower D,Aihara H,Bardwell VJ

Interaction between DMRT1 function and genetic background modulates signaling and pluripotency to control tumor susceptibility in the fetal germ line.
Developmental biology377(1)67-78(2013 May)
Krentz AD,Murphy MW,Zhang T,Sarver AL,Jain S,Griswold MD,Bardwell VJ,Zarkower D

Analysis of meiosis regulators in human gonads: a sexually dimorphic spatio-temporal expression pattern suggests involvement of DMRT1 in meiotic entry.
Molecular human reproduction18(11)523-34(2012 Nov)
J?rgensen A,Nielsen JE,Blomberg Jensen M,Gr?m N,Rajpert-De Meyts E

DMRT1 promotes oogenesis by transcriptional activation of Stra8 in the mammalian fetal ovary.
Developmental biology356(1)63-70(2011 Aug)
Krentz AD,Murphy MW,Sarver AL,Griswold MD,Bardwell VJ,Zarkower D

DMRT1 prevents female reprogramming in the postnatal mammalian testis.
Nature476(7358)101-4(2011 Jul)
Matson CK,Murphy MW,Sarver AL,Griswold MD,Bardwell VJ,Zarkower D

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Dmrt1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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