Selenof cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Selenof
Entrez Gene ID	113922
Full Name	selenoprotein F
Synonyms	Sep15
General protein information	Preferred Names selenoprotein F Names selenoprotein F 15 kDa selenoprotein 15 kDa selenoprotein-like selenoprotein 15
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q44
Summary	The protein encoded by this gene belongs to the SEP15/selenoprotein M family. The exact function of this protein is not known; however, it has been found to associate with UDP-glucose:glycoprotein glucosyltransferase (UGTR), an endoplasmic reticulum(ER)-resident protein, which is involved in the quality control of protein folding. The association with UGTR retains this protein in the ER, where it may play a role in protein folding. Knockout studies in mice also suggest a role for this gene in cataract formation and colon carcinogenesis. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec). Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. [provided by RefSeq, Nov 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_133297.2	NP_579831.2	selenoprotein F precursor

Pathways from BioSystems

Homologies

	SEP15	NP_001152888.1
Bos taurus (cattle)	SEP15	NP_001029931.2
Gallus gallus (chicken)	SEP15	NP_001012944.2
Arabidopsis thaliana (thale cress)	AT1G05720	NP_563747.1
Xenopus tropicalis (tropical clawed frog)	sep15	NP_001165182.1
Homo sapiens (human)	SEP15	NP_004252.2
Pan troglodytes (chimpanzee)	SEP15	NP_001139315.1
Rattus norvegicus (Norway rat)	Sep15	NP_579831.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004986	XP_315090.2
Oryza sativa (rice)	Os01g0894500	NP_001045069.1
Canis lupus familiaris (dog)	SEP15	NP_001108232.1
Mus musculus (house mouse)	Sep15	NP_444332.1
Danio rerio (zebrafish)	sep15	NP_840079.1
Drosophila melanogaster (fruit fly)	CG7484	NP_649000.1
Caenorhabditis elegans (roundworm)	Y76B12C.3	NP_500152.1

Gene Ontology
Bibliography

Related articles in PubMed

Selenite and ebselen supplementation attenuates D-galactose-induced oxidative stress and increases expression of SELR and SEP15 in rat lens.
Dai J, Zhou J, Liu H, Huang K
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry21(8)1037-1046(2016 Dec)

Selenoprotein Gene Nomenclature.
Gladyshev VN, Arn?r ES, Berry MJ, Brigelius-Floh? R, Bruford EA, Burk RF, Carlson BA, Castellano S, Chavatte L, Conrad M, Copeland PR, Diamond AM, Driscoll DM, Ferreiro A, Floh? L, Green FR, Guig? R, Handy DE, Hatfield DL, Hesketh J, Hoffmann PR, Holmgren A, Hondal RJ, Howard MT, Huang K, Kim HY, Kim IY, K?hrle J, Krol A, Kryukov GV, Lee BJ, Lee BC, Lei XG, Liu Q, Lescure A, Lobanov AV, Loscalzo J, Maiorino M, Mariotti M, Sandeep Prabhu K, Rayman MP, Rozovsky S, Salinas G, Schmidt EE, Schomburg L, Schweizer U, Simonovi? M, Sunde RA, Tsuji PA, Tweedie S, Ursini F, Whanger PD, Zhang Y
The Journal of biological chemistry291(46)24036-24040(2016 Nov)

Ribosomal protein L30 is a component of the UGA-selenocysteine recoding machinery in eukaryotes.
Chavatte L, Brown BA, Driscoll DM
Nature structural & molecular biology12(5)408-16(2005 May)

Prevention against oxidative stress of eukaryotic cell membranes by selenium compounds of the rat.
Kyriakopoulos A, Bukalis K, Roethlein D, Hoppe B, Graebert A, Behne D
Annals of the New York Academy of Sciences1030458-61(2004 Dec)

Genetic and functional analysis of mammalian Sep15 selenoprotein.
Kumaraswamy E, Korotkov KV, Diamond AM, Gladyshev VN, Hatfield DL
Methods in enzymology347187-97(2002)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that 15kD selenoprotein (SEP15) protein and selenoprotein R (SELR) protein expression was decreased in the lenses of galactose induced cataract.
Title: Selenite and ebselen supplementation attenuates D-galactose-induced oxidative stress and increases expression of SELR and SEP15 in rat lens.

Genetic and functional analysis of mammalian Sep15 selenoprotein.
Title: Genetic and functional analysis of mammalian Sep15 selenoprotein.

The following Selenof gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Selenof cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13149	NM_133297.2 Latest version!	Rattus norvegicus selenoprotein F (Selenof), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Selenof ( NM_133297.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_579831.2 Rattus norvegicus selenoprotein F (Selenof), mRNA.

Price & Availability↑

CloneID	ORa13149
Clone ID Related Accession (Same CDS sequence)	NM_133297.2
Accession Version	NM_133297.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	selenoprotein F precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CK596377.1, BC060547.1, DY561321.1 and AI112281.1. On Aug 8, 2006 this sequence version replaced NM_133297.1. ##Evidence-Data-START## Transcript exon combination :: CK596377.1, FQ212727.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCAG GGCAGGGCGG GTGGCTCCGG CCCGCGCTGG GGCTTCGCTT ACTGCTGGCG 
ACTGCGTTTC AAGCGGTGTC TGCTCTTGGG GCAGAGTTCT CGTCAGAGGC ATGCCGGGAG 
TTGGGCTTCT CCAGCAACTT GCTCTGCAGC TCCTGCGATC TCCTTGGACA GTTTAACCTG 
CTTCCACTGG ATCCTGTCTG CAGAGGCTGC TGTCAGGAAG AAGCGCAGTT TGAAACCAAA 
AAGCTGTATG CAGGAGCCAT CCTTGAAGTC TGTGGATGAA AATTGGGGAG GTTCCCTCAA 
GTCCAAGCTT TTGTCAGAAG CGATAAACCC AAACTGTTCA GAGGTCTACA GATCAAGTAT 
GTTCGAGGCT CAGACCCTGT ACTAAAGCTT TTGGACGACA ACGGGAACAT TGCTGAAGAG 
CTCAGCATCC TCAAGTGGAA CACAGACAGT GTGGAAGAGT TCCTGAGCGA GAAGCTGGAA 
CGCATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_579831.2
CDS	277..765
Translation	MAAGQGGWLRPALGLRLLLATAFQAVSALGAEFSSEACRELGFSSNLLCSSCDLLGQFNLLPLDPVCRGCCQEEAQFETKKLYAGAILEVCG*KLGRFPQVQAFVRSDKPKLFRGLQIKYVRGSDPVLKLLDDNGNIAEELSILKWNTDSVEEFLSEKLERI

Target ORF information:

RefSeq Version	NM_133297.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus selenoprotein F (Selenof), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133297.2

ORF Insert Sequence:

ATGGCGGCAG GGCAGGGCGG GTGGCTCCGG CCCGCGCTGG GGCTTCGCTT ACTGCTGGCG 
ACTGCGTTTC AAGCGGTGTC TGCTCTTGGG GCAGAGTTCT CGTCAGAGGC ATGCCGGGAG 
TTGGGCTTCT CCAGCAACTT GCTCTGCAGC TCCTGCGATC TCCTTGGACA GTTTAACCTG 
CTTCCACTGG ATCCTGTCTG CAGAGGCTGC TGTCAGGAAG AAGCGCAGTT TGAAACCAAA 
AAGCTGTATG CAGGAGCCAT CCTTGAAGTC TGTGGATGAA AATTGGGGAG GTTCCCTCAA 
GTCCAAGCTT TTGTCAGAAG CGATAAACCC AAACTGTTCA GAGGTCTACA GATCAAGTAT 
GTTCGAGGCT CAGACCCTGT ACTAAAGCTT TTGGACGACA ACGGGAACAT TGCTGAAGAG 
CTCAGCATCC TCAAGTGGAA CACAGACAGT GTGGAAGAGT TCCTGAGCGA GAAGCTGGAA 
CGCATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Selenite and ebselen supplementation attenuates D-galactose-induced oxidative stress and increases expression of SELR and SEP15 in rat lens.
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry21(8)1037-1046(2016 Dec)
Dai J,Zhou J,Liu H,Huang K

Selenoprotein Gene Nomenclature.
The Journal of biological chemistry291(46)24036-24040(2016 Nov)
Gladyshev VN,Arn?r ES,Berry MJ,Brigelius-Floh? R,Bruford EA,Burk RF,Carlson BA,Castellano S,Chavatte L,Conrad M,Copeland PR,Diamond AM,Driscoll DM,Ferreiro A,Floh? L,Green FR,Guig? R,Handy DE,Hatfield DL,Hesketh J,Hoffmann PR,Holmgren A,Hondal RJ,Howard MT,Huang K,Kim HY,Kim IY,K?hrle J,Krol A,Kryukov GV,Lee BJ,Lee BC,Lei XG,Liu Q,Lescure A,Lobanov AV,Loscalzo J,Maiorino M,Mariotti M,Sandeep Prabhu K,Rayman MP,Rozovsky S,Salinas G,Schmidt EE,Schomburg L,Schweizer U,Simonovi? M,Sunde RA,Tsuji PA,Tweedie S,Ursini F,Whanger PD,Zhang Y

Ribosomal protein L30 is a component of the UGA-selenocysteine recoding machinery in eukaryotes.
Nature structural & molecular biology12(5)408-16(2005 May)
Chavatte L,Brown BA,Driscoll DM

Prevention against oxidative stress of eukaryotic cell membranes by selenium compounds of the rat.
Annals of the New York Academy of Sciences1030458-61(2004 Dec)
Kyriakopoulos A,Bukalis K,Roethlein D,Hoppe B,Graebert A,Behne D

Genetic and functional analysis of mammalian Sep15 selenoprotein.
Methods in enzymology347187-97(2002)
Kumaraswamy E,Korotkov KV,Diamond AM,Gladyshev VN,Hatfield DL

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Selenof cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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