Adh5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Adh5
Entrez Gene ID	100145871
Full Name	alcohol dehydrogenase 5 (class III), chi polypeptide
General protein information	Preferred Names alcohol dehydrogenase 5 (class III), chi polypeptide Names alcohol dehydrogenase class-3 ADH-B2 FALDH FDH GSH-FDH S-(hydroxymethyl)glutathione dehydrogenase alcohol dehydrogenase 2 alcohol dehydrogenase B2 alcohol dehydrogenase class-III class III alcohol dehydrogenase, chi subunit glutathione-dependent formaldehyde dehydrogenase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q44

mRNA and Protein(s)

mRNA	Protein	Name
NM_001126120.1	NP_001119592.1	alcohol dehydrogenase class-3

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ADH5	NP_001029421.1
Eremothecium gossypii	AGOS_ACL148C	NP_983256.1
Schizosaccharomyces pombe (fission yeast)	SPCC13B11.04c	NP_588247.1
Xenopus tropicalis (tropical clawed frog)	adh5	NP_001011502.1
Canis lupus familiaris (dog)	ADH5	NP_001239084.1
Saccharomyces cerevisiae (baker's yeast)	SFA1	NP_010113.1
Kluyveromyces lactis	KLLA0D12342g	XP_453612.1
Oryza sativa (rice)	Os02g0815500	NP_001048503.1
Homo sapiens (human)	ADH5	NP_000662.3
Pan troglodytes (chimpanzee)	ADH5	XP_001166206.1
Mus musculus (house mouse)	Adh5	NP_031436.2
Rattus norvegicus (Norway rat)	Adh5	NP_001119592.1
Gallus gallus (chicken)	ADH5	NP_001026323.1
Caenorhabditis elegans (roundworm)	H24K24.3	NP_001024016.1
Caenorhabditis elegans (roundworm)	Y50D4C.2	NP_503357.1
Magnaporthe oryzae (rice blast fungus)	MGG_06011	XP_003711858.1
	LOC697703	XP_001088376.1
Danio rerio (zebrafish)	adh5	NP_571924.2
Drosophila melanogaster (fruit fly)	Fdh	NP_524310.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010499	XP_314472.4
Neurospora crassa	NCU06652	XP_960697.1
Arabidopsis thaliana (thale cress)	HOT5	NP_199207.1

Gene Ontology
Bibliography

Related articles in PubMed

Nebivolol Acts as a S-Nitrosoglutathione Reductase Inhibitor: A New Mechanism of Action.
Jiang H, Polhemus DJ, Islam KN, Torregrossa AC, Li Z, Potts A, Lefer DJ, Bryan NS
Journal of cardiovascular pharmacology and therapeutics21(5)478-85(2016 Sep)

Activation of GSNOR transcription by NF-?B negatively regulates NGF-induced PC12 differentiation.
Wu K, Zhang Y, Wang P, Zhang L, Wang T, Chen C
Free radical research48(9)1011-7(2014 Sep)

Analysis of mammalian alcohol dehydrogenase 5 (ADH5): characterisation of rat ADH5 with comparisons to the corresponding human variant.
Ostberg LJ, Str?mberg P, Hedberg JJ, Persson B, H??g JO
Chemico-biological interactions202(1-3)97-103(2013 Feb)

Inhibition of nitric oxide synthesis during induced cholestasis ameliorates hepatocellular injury by facilitating S-nitrosothiol homeostasis.
L?pez-S?nchez LM, Corrales FJ, Barcos M, Espejo I, Mu?oz-Casta?eda JR, Rodr?guez-Ariza A
Laboratory investigation; a journal of technical methods and pathology90(1)116-27(2010 Jan)

Increased nitrosoglutathione reductase activity in hypoxic pulmonary hypertension in mice.
Wu X, Du L, Xu X, Tan L, Li R
Journal of pharmacological sciences113(1)32-40(2010)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Report inhibition of GSNOR activity by nebivolol leading to accumulation of nitrosothiols in cells, and this is associated with an enhanced vasodilation by S-nitrosoglutathione.
Title: Nebivolol Acts as a S-Nitrosoglutathione Reductase Inhibitor: A New Mechanism of Action.

We have defined a novel role of GSNOR in neurite development and provide molecular insights into the control of neurite growth.
Title: Activation of GSNOR transcription by NF-?B negatively regulates NGF-induced PC12 differentiation.

ADH5, while readily expressed at the mRNA level, does not behave similarly to other mammalian ADHs investigated.
Title: Analysis of mammalian alcohol dehydrogenase 5 (ADH5): characterisation of rat ADH5 with comparisons to the corresponding human variant.

The following Adh5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Adh5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01339	NM_001126120.1 Latest version!	Rattus norvegicus alcohol dehydrogenase 5 (class III), chi polypeptide (Adh5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Adh5 ( NM_001126120.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001119592.1 Rattus norvegicus alcohol dehydrogenase 5 (class III), chi polypeptide (Adh5), mRNA.

Price & Availability↑

CloneID	ORa01339
Clone ID Related Accession (Same CDS sequence)	NM_001126120.1
Accession Version	NM_001126120.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	alcohol dehydrogenase class-3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC083724.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC083724.1, FQ212535.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCGAACC AGGTGATCAG GTGTAAGGCT GCAGTCGCCT GGGAGGCCGG AAAGCCTCTC 
TCCATAGAGG AGATAGAGGT GGCCCCTCCA CAGGCTCATG AAGTTCGAAT TAAGATCATT 
GCCACTGCTG TTTGCCACAC CGATGCCTAT ACCCTGAGTG GAGCTGATCC TGAGGGGTGT 
TTCCCCGTGA TCTTAGGACA TGAAGGCGCT GGCATTGTGG AGAGTGTTGG CGAAGGGGTT 
ACTAAGCTGA AGGCAGGTGA TACCGTCATC CCGCTGTACA TCCCACAGTG TGGAGAATGC 
AAGTTCTGTC TGAATCCTAA AACAAACCTT TGCCAGAAAA TAAGAGTCAC TCAGGGGAAA 
GGGTTGATGC CCGATGGGAC CAGCAGGTTT ACCTGCAAAG GGAAGCCTAT TCTTCACTTC 
ATGGGAACCA GCACGTTTTC CGAGTACACA GTGGTGGCAG ACATCTCTGT TGCTAAAATT 
GACCCTTCCG CCCCTTTGGA TAAAGTCTGC CTTCTCGGTT GTGGCATTTC AACTGGCTAC 
GGGGCCGCTG TGAACACTGC CAAGGTGGAG CCTGGTTCTA CCTGTGCCGT CTTTGGCCTG 
GGAGGAGTTG GATTGGCGGT GATCATGGGC TGTAAAGTGG CTGGTGCGTC CCGGATCATT 
GGTATTGACA TCAATAAAGA TAAATTCGCG AAGGCCAAAG AATTTGGAGC CACTGAATGT 
ATTAACCCCC AAGATTTCAG TAAATCCATC CAGGAAGTCC TCATCGAGAT GACAGATGGC 
GGAGTGGACT TCTCCTTTGA GTGTATCGGT AACGTGAAGG TCATGAGATC AGCCCTCGAG 
GCAGCCCACA AAGGCTGGGG TGTCAGTGTG GTGGTTGGTG TGGCCGCTTC AGGTGAAGAA 
ATCTCCACTC GTCCGTTCCA ACTGGTGACA GGACGCACGT GGAAAGGCAC TGCCTTTGGA 
GGCTGGAAGA GTGTGGAGAG TGTCCCGAAG CTGGTGTCTG AATATATGTC CAAAAAGATA 
AAAGTTGACG AATTTGTGAC TGGCAATCTG TCCTTCGACC AGATTAACAA AGCCTTTGAT 
CTGATGCACT CGGGAAACAG CATTCGAACT GTTCTAAAGA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001119592.1
CDS	16..1140
Translation	MANQVIRCKAAVAWEAGKPLSIEEIEVAPPQAHEVRIKIIATAVCHTDAYTLSGADPEGCFPVILGHEGAGIVESVGEGVTKLKAGDTVIPLYIPQCGECKFCLNPKTNLCQKIRVTQGKGLMPDGTSRFTCKGKPILHFMGTSTFSEYTVVADISVAKIDPSAPLDKVCLLGCGISTGYGAAVNTAKVEPGSTCAVFGLGGVGLAVIMGCKVAGASRIIGIDINKDKFAKAKEFGATECINPQDFSKSIQEVLIEMTDGGVDFSFECIGNVKVMRSALEAAHKGWGVSVVVGVAASGEEISTRPFQLVTGRTWKGTAFGGWKSVESVPKLVSEYMSKKIKVDEFVTGNLSFDQINKAFDLMHSGNSIRTVLKM

Target ORF information:

RefSeq Version	NM_001126120.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alcohol dehydrogenase 5 (class III), chi polypeptide (Adh5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001126120.1

ORF Insert Sequence:

ATGGCGAACC AGGTGATCAG GTGTAAGGCT GCAGTCGCCT GGGAGGCCGG AAAGCCTCTC 
TCCATAGAGG AGATAGAGGT GGCCCCTCCA CAGGCTCATG AAGTTCGAAT TAAGATCATT 
GCCACTGCTG TTTGCCACAC CGATGCCTAT ACCCTGAGTG GAGCTGATCC TGAGGGGTGT 
TTCCCCGTGA TCTTAGGACA TGAAGGCGCT GGCATTGTGG AGAGTGTTGG CGAAGGGGTT 
ACTAAGCTGA AGGCAGGTGA TACCGTCATC CCGCTGTACA TCCCACAGTG TGGAGAATGC 
AAGTTCTGTC TGAATCCTAA AACAAACCTT TGCCAGAAAA TAAGAGTCAC TCAGGGGAAA 
GGGTTGATGC CCGATGGGAC CAGCAGGTTT ACCTGCAAAG GGAAGCCTAT TCTTCACTTC 
ATGGGAACCA GCACGTTTTC CGAGTACACA GTGGTGGCAG ACATCTCTGT TGCTAAAATT 
GACCCTTCCG CCCCTTTGGA TAAAGTCTGC CTTCTCGGTT GTGGCATTTC AACTGGCTAC 
GGGGCCGCTG TGAACACTGC CAAGGTGGAG CCTGGTTCTA CCTGTGCCGT CTTTGGCCTG 
GGAGGAGTTG GATTGGCGGT GATCATGGGC TGTAAAGTGG CTGGTGCGTC CCGGATCATT 
GGTATTGACA TCAATAAAGA TAAATTCGCG AAGGCCAAAG AATTTGGAGC CACTGAATGT 
ATTAACCCCC AAGATTTCAG TAAATCCATC CAGGAAGTCC TCATCGAGAT GACAGATGGC 
GGAGTGGACT TCTCCTTTGA GTGTATCGGT AACGTGAAGG TCATGAGATC AGCCCTCGAG 
GCAGCCCACA AAGGCTGGGG TGTCAGTGTG GTGGTTGGTG TGGCCGCTTC AGGTGAAGAA 
ATCTCCACTC GTCCGTTCCA ACTGGTGACA GGACGCACGT GGAAAGGCAC TGCCTTTGGA 
GGCTGGAAGA GTGTGGAGAG TGTCCCGAAG CTGGTGTCTG AATATATGTC CAAAAAGATA 
AAAGTTGACG AATTTGTGAC TGGCAATCTG TCCTTCGACC AGATTAACAA AGCCTTTGAT 
CTGATGCACT CGGGAAACAG CATTCGAACT GTTCTAAAGA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nebivolol Acts as a S-Nitrosoglutathione Reductase Inhibitor: A New Mechanism of Action.
Journal of cardiovascular pharmacology and therapeutics21(5)478-85(2016 Sep)
Jiang H,Polhemus DJ,Islam KN,Torregrossa AC,Li Z,Potts A,Lefer DJ,Bryan NS

Activation of GSNOR transcription by NF-?B negatively regulates NGF-induced PC12 differentiation.
Free radical research48(9)1011-7(2014 Sep)
Wu K,Zhang Y,Wang P,Zhang L,Wang T,Chen C

Analysis of mammalian alcohol dehydrogenase 5 (ADH5): characterisation of rat ADH5 with comparisons to the corresponding human variant.
Chemico-biological interactions202(1-3)97-103(2013 Feb)
Ostberg LJ,Str?mberg P,Hedberg JJ,Persson B,H??g JO

Inhibition of nitric oxide synthesis during induced cholestasis ameliorates hepatocellular injury by facilitating S-nitrosothiol homeostasis.
Laboratory investigation; a journal of technical methods and pathology90(1)116-27(2010 Jan)
L?pez-S?nchez LM,Corrales FJ,Barcos M,Espejo I,Mu?oz-Casta?eda JR,Rodr?guez-Ariza A

Increased nitrosoglutathione reductase activity in hypoxic pulmonary hypertension in mice.
Journal of pharmacological sciences113(1)32-40(2010)
Wu X,Du L,Xu X,Tan L,Li R