BUB1 cDNA ORF clone, Pteropus vampyrus(large flying fox)

Gene
Clones
gRNAs

Summary

Gene Symbol	BUB1
Entrez Gene ID	105303971
Full Name	BUB1 mitotic checkpoint serine/threonine kinase
Gene Type	protein-coding
Organism	Pteropus vampyrus(large flying fox)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011377851.1	XP_011376153.1	mitotic checkpoint serine/threonine-protein kinase BUB1
XM_011377851.2	XP_011376153.1	mitotic checkpoint serine/threonine-protein kinase BUB1

Pathways from BioSystems
Gene Ontology

The following BUB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BUB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPt59541	XM_011377851.2 Latest version!	Pteropus vampyrus BUB1 mitotic checkpoint serine/threonine kinase (BUB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OPt59541	XM_011377851.1	Pteropus vampyrus BUB1 mitotic checkpoint serine/threonine kinase (BUB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BUB1 ( XM_011377851.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011376153.1 Pteropus vampyrus BUB1 mitotic checkpoint serine/threonine kinase (BUB1), mRNA.

Price & Availability↑

CloneID	OPt59541
Clone ID Related Accession (Same CDS sequence)	XM_011377851.1 , XM_011377851.2
Accession Version	XM_011377851.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-22
Organism	Pteropus vampyrus(large flying fox)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011889104.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACC CGGAAAACGC CTTCCAAATG TTCGAAGCCC ATATGCAGCG GTACAAGGGC 
GATGACCCAC TTGGTGAATG GGAAAGTTAC ATGCAGTGGG TAGAAGAGAA TTTTCCTGAG 
AATAAAGAAT ACTCGACAAC TCTATTAGAG CAGTTAATGA AGGAGTTTTT AGATAAGAAG 
AGATACCACG ATGACCCAAG ATTCATCAAC TATTGTTTAA AATTTGCTGA GTGCAACAGT 
GACCGTCATC AGTTTTTTGA GTTTCTGTAC AACCATGGGA TTGGGGCACG GTGCTCCCAC 
CTGTATATAG CCTGGGCCGC ACACCTGGAG GCCAGTGGAG AGCTGCAGCT CGCTGGCGCT 
GTCTTCCGGA GGGGCCTGCA GAGCCGGGCG GAGCCAGGCG AGCCACTGCG GCAGCAGTAC 
AGGTTATTTC AGACACGTGT CACTGAAACC CAGTTGCCAG CTCAAGCTAG AACCTCAGAA 
CCTCTGCATG ATGCTCAGAT TTTAAACCAG ATGATGACAT CAAAACCAAA TCCAGGAAAT 
AATGCAGCCT GTGTTTCTAA GCTTCAGGGT TCAGAAGCTT CTGGAGTAAC ATCTTCAGCT 
TGTGATAAAG AGTCGAATAT GGAACAAAGA GTGGTCATGA TTTCTAAATC GGGTTACTCT 
GCACAGCCCT CTCTGGCAGC CAGAGCAGAC ATCCAGCAGA TGGTTATGTA CTGCAAGGCA 
AAGCTCATTC GTGGGGACTC AGAATTCTCC TTTGAAGAGC TGAGAGCCCA GAAGTACAAT 
CAGCGGAGAA AGCATGAGCA GTGGGTAAAT GAAGATAGAC ATTACATGAA AAGGAAAGAA 
GCAAATGCTT TTGAAGAACA GCTATTGAAA GAGAAAATGG ATGAACTTCA TAAGAAGTTG 
CATCAAGTGG CGGAGCCACC ACAGGAGAGC CTGCCTGCTT TCCAGGAGAG TCCTGAGGTT 
GACCCACTGT GCATGGGGCC CAGTGTCGGC TCCCAGCAAG AACCGAGTAC TCCGTCTCTG 
CCGGCTGTCA CCCAGCAGAC TTCAGAGCAC CTGGGACAGA GACCCAGAGA GGCACCTCCA 
GCTGTTCCTC CTGAGGCAAA TGCTATCACT GTCACTTTGG TGTCCCCAGC TGTCAGCCAG 
AGTATGATTC CTGCTCCTTT GACATCACAG CCAGTGACAG ACTCCATGTT TGCAGCAGCC 
GGAAAAGATG CCAGATGTGT GACCAGAAGT ACTCATGGAT TCAAGCCGCC GAGCGGAGCA 
GAGCTGACTG GAGGGTGTGA CACACACAAG GTTGTGAACA CAGGTTCTTT TCACACGACT 
CCAAACACGT CGCTGGGAGT GGTCCAGGCA ACGCCGTCCA CGGGGCAGCC ATCCCCCACC 
GTGCACACGA AGGAAGCCTT AGGCTTCATC ATGAATGTGT TCCAGGCTCC TACACTGCCT 
GATGTGTCTG ATGACCAAGA TGAATGGCCA TCTCTAGATC AAAATGAAGA TGCATTCGAG 
GCCCAGTTTC AAAAAAATGC AAGTTCTTCT GGGGCTTGGG GCGTCAATAA GGTTATCTCT 
TCTTTATCAT CTGCTTTTTC TGTGTTTGAA GATGGAAACA AAGAAAATTA TGGGTTACCC 
CAGCCTAAGA ATAAACCCGC AGGAGCCAGG ACCTTTGGAG AACGCAGTGT AAGCAGATTT 
TCCCCTAAAC CAAAGGAAGT GCCATGTCCT GAGGAGTTTC CTGATGACTC GACTGTCTGG 
GGCGTCCGCT GCCCCAAAAT GCTGGCGCCC AGCCCCAGGA GCCCAGGAGG CTTTACATCC 
GCCGCACGGC TTGCATCCAC GCCTTCCCAT AAGCTTCTGG TGGGCCCAGT GCATGCTCTA 
GAGGACAAAG AAAATGTGGT AGCAGCACAG TACGCCCATG TGAATTTGGA TGCTCATGAA 
GGAAACGTGG CGCAACCCTC AGAAGACAGG AAGTTCAGCC CAATGCAGGA GGGAAGCCTA 
GCAGCAGCTG CAGGCGGCGC GCTGACCAGA GAGGCAGAGC GGGGCCTGGC TGCCTGCACC 
CTCACAGACG CTGACCTTGC CACGGCGGCG GGGTGCACAG ACACCGGCAC TGGACTTGAG 
GCAGAGCAGG CACAGCCTCG TTCACCAGAC CTTGCTGTTG AGAATCCGTG GGATGGTGAA 
CTGATTCTCA AACTTCTCTC CGGGCTTCCT CAGCCCTTGC ACTCCCATCC AGACGTTTTT 
GAGTGGCAGT GCAAGCTCCC TGCCATGAAG CCCAAGACCG AGTTCCAGCT GGGTTCTTTG 
CTGGTCTACA TTGATCACCT TCTCGGAGAA GGGGCTTTTG CCCAAGTTTA TGAAGTTACC 
CATGGGGATG TGAAGGATAC CAAAAGCAAA CAGAAATTTG TTCTGAAGGT CCAGAAGCCC 
GCCAACCCCT GGGAGTTCTA CATTGGGACT CAGCTGATGG AGAGGCTGAA GCCCAGTGTG 
CGACACATGT TTATCAGGTT CTACTCCGCC CACTTCTTCC GGGATGGCAG CGTGCTGGTG 
GGGGATCTTC ACAGCTACGG GACGCTGCTG AATGCCATCA ACCTCTATAA AAATACCCCT 
GAGAAAGTGA TGCCTCAAGC TCTGGTGCTG TCCTTTGCTA TCAGCATTCT CCACATGATA 
GAACAGGTCC ACGGCTGCGA GATCATCCAT GGGGATGTCA AACCAGACAA CTTCATACTC 
GGGAGCAGAG TTTTGGAACA AGACAGCGAA GATGATGGTT TATCTGCCGG CCTGGCACTG 
ATTGACCTGG GTCAGAGCAT AGACATGAAA CTTTTCCCAA AAGGAACTAC ATTCACAGGA 
AAGTGTGAAA CGTCCGGTTT CCAGTGTACG GAGATGCTCA GTAACAAACC GTGGACCTAC 
CAGATTGATT ACTTTGGAGT TGCAGCAACA GTGTACTGCA TGCTTTTTGG CTCTTACATG 
AAAGTGAAAA ACGAAGGCGG AGTCTGGAAG CCTGAAGGTC TTTTTAGAAG GCTTCCTCAT 
CTGGACATGT GGAATGAATT TTTTCACATC ATGTTGAATA TACCAGATTG TCATCATCTT 
CCATCTTTGG ATTTGTTAAG GCAAAAGCTG AAGAAAATAT TTCAGCAGCA CTATACAAAC 
AAGATTAAGA CTCTACGTAA TAGGCTAATT GTTCTGCTCT TAGAATATAA GCGTTCTCGA 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011376153.1
CDS	92..3277
Translation	MDDPENAFQMFEAHMQRYKGDDPLGEWESYMQWVEENFPENKEYSTTLLEQLMKEFLDKKRYHDDPRFINYCLKFAECNSDRHQFFEFLYNHGIGARCSHLYIAWAAHLEASGELQLAGAVFRRGLQSRAEPGEPLRQQYRLFQTRVTETQLPAQARTSEPLHDAQILNQMMTSKPNPGNNAACVSKLQGSEASGVTSSACDKESNMEQRVVMISKSGYSAQPSLAARADIQQMVMYCKAKLIRGDSEFSFEELRAQKYNQRRKHEQWVNEDRHYMKRKEANAFEEQLLKEKMDELHKKLHQVAEPPQESLPAFQESPEVDPLCMGPSVGSQQEPSTPSLPAVTQQTSEHLGQRPREAPPAVPPEANAITVTLVSPAVSQSMIPAPLTSQPVTDSMFAAAGKDARCVTRSTHGFKPPSGAELTGGCDTHKVVNTGSFHTTPNTSLGVVQATPSTGQPSPTVHTKEALGFIMNVFQAPTLPDVSDDQDEWPSLDQNEDAFEAQFQKNASSSGAWGVNKVISSLSSAFSVFEDGNKENYGLPQPKNKPAGARTFGERSVSRFSPKPKEVPCPEEFPDDSTVWGVRCPKMLAPSPRSPGGFTSAARLASTPSHKLLVGPVHALEDKENVVAAQYAHVNLDAHEGNVAQPSEDRKFSPMQEGSLAAAAGGALTREAERGLAACTLTDADLATAAGCTDTGTGLEAEQAQPRSPDLAVENPWDGELILKLLSGLPQPLHSHPDVFEWQCKLPAMKPKTEFQLGSLLVYIDHLLGEGAFAQVYEVTHGDVKDTKSKQKFVLKVQKPANPWEFYIGTQLMERLKPSVRHMFIRFYSAHFFRDGSVLVGDLHSYGTLLNAINLYKNTPEKVMPQALVLSFAISILHMIEQVHGCEIIHGDVKPDNFILGSRVLEQDSEDDGLSAGLALIDLGQSIDMKLFPKGTTFTGKCETSGFQCTEMLSNKPWTYQIDYFGVAATVYCMLFGSYMKVKNEGGVWKPEGLFRRLPHLDMWNEFFHIMLNIPDCHHLPSLDLLRQKLKKIFQQHYTNKIKTLRNRLIVLLLEYKRSRK

Target ORF information:

RefSeq Version	XM_011377851.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus BUB1 mitotic checkpoint serine/threonine kinase (BUB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011377851.1

ORF Insert Sequence:

ATGGATGACC CGGAAAACGC CTTCCAAATG TTCGAAGCCC ATATGCAGCG GTACAAGGGC 
GATGACCCAC TTGGTGAATG GGAAAGTTAC ATGCAGTGGG TAGAAGAGAA TTTTCCTGAG 
AATAAAGAAT ACTCGACAAC TCTATTAGAG CAGTTAATGA AGGAGTTTTT AGATAAGAAG 
AGATACCACG ATGACCCAAG ATTCATCAAC TATTGTTTAA AATTTGCTGA GTGCAACAGT 
GACCGTCATC AGTTTTTTGA GTTTCTGTAC AACCATGGGA TTGGGGCACG GTGCTCCCAC 
CTGTATATAG CCTGGGCCGC ACACCTGGAG GCCAGTGGAG AGCTGCAGCT CGCTGGCGCT 
GTCTTCCGGA GGGGCCTGCA GAGCCGGGCG GAGCCAGGCG AGCCACTGCG GCAGCAGTAC 
AGGTTATTTC AGACACGTGT CACTGAAACC CAGTTGCCAG CTCAAGCTAG AACCTCAGAA 
CCTCTGCATG ATGCTCAGAT TTTAAACCAG ATGATGACAT CAAAACCAAA TCCAGGAAAT 
AATGCAGCCT GTGTTTCTAA GCTTCAGGGT TCAGAAGCTT CTGGAGTAAC ATCTTCAGCT 
TGTGATAAAG AGTCGAATAT GGAACAAAGA GTGGTCATGA TTTCTAAATC GGGTTACTCT 
GCACAGCCCT CTCTGGCAGC CAGAGCAGAC ATCCAGCAGA TGGTTATGTA CTGCAAGGCA 
AAGCTCATTC GTGGGGACTC AGAATTCTCC TTTGAAGAGC TGAGAGCCCA GAAGTACAAT 
CAGCGGAGAA AGCATGAGCA GTGGGTAAAT GAAGATAGAC ATTACATGAA AAGGAAAGAA 
GCAAATGCTT TTGAAGAACA GCTATTGAAA GAGAAAATGG ATGAACTTCA TAAGAAGTTG 
CATCAAGTGG CGGAGCCACC ACAGGAGAGC CTGCCTGCTT TCCAGGAGAG TCCTGAGGTT 
GACCCACTGT GCATGGGGCC CAGTGTCGGC TCCCAGCAAG AACCGAGTAC TCCGTCTCTG 
CCGGCTGTCA CCCAGCAGAC TTCAGAGCAC CTGGGACAGA GACCCAGAGA GGCACCTCCA 
GCTGTTCCTC CTGAGGCAAA TGCTATCACT GTCACTTTGG TGTCCCCAGC TGTCAGCCAG 
AGTATGATTC CTGCTCCTTT GACATCACAG CCAGTGACAG ACTCCATGTT TGCAGCAGCC 
GGAAAAGATG CCAGATGTGT GACCAGAAGT ACTCATGGAT TCAAGCCGCC GAGCGGAGCA 
GAGCTGACTG GAGGGTGTGA CACACACAAG GTTGTGAACA CAGGTTCTTT TCACACGACT 
CCAAACACGT CGCTGGGAGT GGTCCAGGCA ACGCCGTCCA CGGGGCAGCC ATCCCCCACC 
GTGCACACGA AGGAAGCCTT AGGCTTCATC ATGAATGTGT TCCAGGCTCC TACACTGCCT 
GATGTGTCTG ATGACCAAGA TGAATGGCCA TCTCTAGATC AAAATGAAGA TGCATTCGAG 
GCCCAGTTTC AAAAAAATGC AAGTTCTTCT GGGGCTTGGG GCGTCAATAA GGTTATCTCT 
TCTTTATCAT CTGCTTTTTC TGTGTTTGAA GATGGAAACA AAGAAAATTA TGGGTTACCC 
CAGCCTAAGA ATAAACCCGC AGGAGCCAGG ACCTTTGGAG AACGCAGTGT AAGCAGATTT 
TCCCCTAAAC CAAAGGAAGT GCCATGTCCT GAGGAGTTTC CTGATGACTC GACTGTCTGG 
GGCGTCCGCT GCCCCAAAAT GCTGGCGCCC AGCCCCAGGA GCCCAGGAGG CTTTACATCC 
GCCGCACGGC TTGCATCCAC GCCTTCCCAT AAGCTTCTGG TGGGCCCAGT GCATGCTCTA 
GAGGACAAAG AAAATGTGGT AGCAGCACAG TACGCCCATG TGAATTTGGA TGCTCATGAA 
GGAAACGTGG CGCAACCCTC AGAAGACAGG AAGTTCAGCC CAATGCAGGA GGGAAGCCTA 
GCAGCAGCTG CAGGCGGCGC GCTGACCAGA GAGGCAGAGC GGGGCCTGGC TGCCTGCACC 
CTCACAGACG CTGACCTTGC CACGGCGGCG GGGTGCACAG ACACCGGCAC TGGACTTGAG 
GCAGAGCAGG CACAGCCTCG TTCACCAGAC CTTGCTGTTG AGAATCCGTG GGATGGTGAA 
CTGATTCTCA AACTTCTCTC CGGGCTTCCT CAGCCCTTGC ACTCCCATCC AGACGTTTTT 
GAGTGGCAGT GCAAGCTCCC TGCCATGAAG CCCAAGACCG AGTTCCAGCT GGGTTCTTTG 
CTGGTCTACA TTGATCACCT TCTCGGAGAA GGGGCTTTTG CCCAAGTTTA TGAAGTTACC 
CATGGGGATG TGAAGGATAC CAAAAGCAAA CAGAAATTTG TTCTGAAGGT CCAGAAGCCC 
GCCAACCCCT GGGAGTTCTA CATTGGGACT CAGCTGATGG AGAGGCTGAA GCCCAGTGTG 
CGACACATGT TTATCAGGTT CTACTCCGCC CACTTCTTCC GGGATGGCAG CGTGCTGGTG 
GGGGATCTTC ACAGCTACGG GACGCTGCTG AATGCCATCA ACCTCTATAA AAATACCCCT 
GAGAAAGTGA TGCCTCAAGC TCTGGTGCTG TCCTTTGCTA TCAGCATTCT CCACATGATA 
GAACAGGTCC ACGGCTGCGA GATCATCCAT GGGGATGTCA AACCAGACAA CTTCATACTC 
GGGAGCAGAG TTTTGGAACA AGACAGCGAA GATGATGGTT TATCTGCCGG CCTGGCACTG 
ATTGACCTGG GTCAGAGCAT AGACATGAAA CTTTTCCCAA AAGGAACTAC ATTCACAGGA 
AAGTGTGAAA CGTCCGGTTT CCAGTGTACG GAGATGCTCA GTAACAAACC GTGGACCTAC 
CAGATTGATT ACTTTGGAGT TGCAGCAACA GTGTACTGCA TGCTTTTTGG CTCTTACATG 
AAAGTGAAAA ACGAAGGCGG AGTCTGGAAG CCTGAAGGTC TTTTTAGAAG GCTTCCTCAT 
CTGGACATGT GGAATGAATT TTTTCACATC ATGTTGAATA TACCAGATTG TCATCATCTT 
CCATCTTTGG ATTTGTTAAG GCAAAAGCTG AAGAAAATAT TTCAGCAGCA CTATACAAAC 
AAGATTAAGA CTCTACGTAA TAGGCTAATT GTTCTGCTCT TAGAATATAA GCGTTCTCGA 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BUB1 ( XM_011377851.2 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011376153.1 Pteropus vampyrus BUB1 mitotic checkpoint serine/threonine kinase (BUB1), mRNA.

Price & Availability↑

CloneID	OPt59541
Clone ID Related Accession (Same CDS sequence)	XM_011377851.1 , XM_011377851.2
Accession Version	XM_011377851.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-18
Organism	Pteropus vampyrus(large flying fox)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011889104.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_011377851.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACC CGGAAAACGC CTTCCAAATG TTCGAAGCCC ATATGCAGCG GTACAAGGGC 
GATGACCCAC TTGGTGAATG GGAAAGTTAC ATGCAGTGGG TAGAAGAGAA TTTTCCTGAG 
AATAAAGAAT ACTCGACAAC TCTATTAGAG CAGTTAATGA AGGAGTTTTT AGATAAGAAG 
AGATACCACG ATGACCCAAG ATTCATCAAC TATTGTTTAA AATTTGCTGA GTGCAACAGT 
GACCGTCATC AGTTTTTTGA GTTTCTGTAC AACCATGGGA TTGGGGCACG GTGCTCCCAC 
CTGTATATAG CCTGGGCCGC ACACCTGGAG GCCAGTGGAG AGCTGCAGCT CGCTGGCGCT 
GTCTTCCGGA GGGGCCTGCA GAGCCGGGCG GAGCCAGGCG AGCCACTGCG GCAGCAGTAC 
AGGTTATTTC AGACACGTGT CACTGAAACC CAGTTGCCAG CTCAAGCTAG AACCTCAGAA 
CCTCTGCATG ATGCTCAGAT TTTAAACCAG ATGATGACAT CAAAACCAAA TCCAGGAAAT 
AATGCAGCCT GTGTTTCTAA GCTTCAGGGT TCAGAAGCTT CTGGAGTAAC ATCTTCAGCT 
TGTGATAAAG AGTCGAATAT GGAACAAAGA GTGGTCATGA TTTCTAAATC GGGTTACTCT 
GCACAGCCCT CTCTGGCAGC CAGAGCAGAC ATCCAGCAGA TGGTTATGTA CTGCAAGGCA 
AAGCTCATTC GTGGGGACTC AGAATTCTCC TTTGAAGAGC TGAGAGCCCA GAAGTACAAT 
CAGCGGAGAA AGCATGAGCA GTGGGTAAAT GAAGATAGAC ATTACATGAA AAGGAAAGAA 
GCAAATGCTT TTGAAGAACA GCTATTGAAA GAGAAAATGG ATGAACTTCA TAAGAAGTTG 
CATCAAGTGG CGGAGCCACC ACAGGAGAGC CTGCCTGCTT TCCAGGAGAG TCCTGAGGTT 
GACCCACTGT GCATGGGGCC CAGTGTCGGC TCCCAGCAAG AACCGAGTAC TCCGTCTCTG 
CCGGCTGTCA CCCAGCAGAC TTCAGAGCAC CTGGGACAGA GACCCAGAGA GGCACCTCCA 
GCTGTTCCTC CTGAGGCAAA TGCTATCACT GTCACTTTGG TGTCCCCAGC TGTCAGCCAG 
AGTATGATTC CTGCTCCTTT GACATCACAG CCAGTGACAG ACTCCATGTT TGCAGCAGCC 
GGAAAAGATG CCAGATGTGT GACCAGAAGT ACTCATGGAT TCAAGCCGCC GAGCGGAGCA 
GAGCTGACTG GAGGGTGTGA CACACACAAG GTTGTGAACA CAGGTTCTTT TCACACGACT 
CCAAACACGT CGCTGGGAGT GGTCCAGGCA ACGCCGTCCA CGGGGCAGCC ATCCCCCACC 
GTGCACACGA AGGAAGCCTT AGGCTTCATC ATGAATGTGT TCCAGGCTCC TACACTGCCT 
GATGTGTCTG ATGACCAAGA TGAATGGCCA TCTCTAGATC AAAATGAAGA TGCATTCGAG 
GCCCAGTTTC AAAAAAATGC AAGTTCTTCT GGGGCTTGGG GCGTCAATAA GGTTATCTCT 
TCTTTATCAT CTGCTTTTTC TGTGTTTGAA GATGGAAACA AAGAAAATTA TGGGTTACCC 
CAGCCTAAGA ATAAACCCGC AGGAGCCAGG ACCTTTGGAG AACGCAGTGT AAGCAGATTT 
TCCCCTAAAC CAAAGGAAGT GCCATGTCCT GAGGAGTTTC CTGATGACTC GACTGTCTGG 
GGCGTCCGCT GCCCCAAAAT GCTGGCGCCC AGCCCCAGGA GCCCAGGAGG CTTTACATCC 
GCCGCACGGC TTGCATCCAC GCCTTCCCAT AAGCTTCTGG TGGGCCCAGT GCATGCTCTA 
GAGGACAAAG AAAATGTGGT AGCAGCACAG TACGCCCATG TGAATTTGGA TGCTCATGAA 
GGAAACGTGG CGCAACCCTC AGAAGACAGG AAGTTCAGCC CAATGCAGGA GGGAAGCCTA 
GCAGCAGCTG CAGGCGGCGC GCTGACCAGA GAGGCAGAGC GGGGCCTGGC TGCCTGCACC 
CTCACAGACG CTGACCTTGC CACGGCGGCG GGGTGCACAG ACACCGGCAC TGGACTTGAG 
GCAGAGCAGG CACAGCCTCG TTCACCAGAC CTTGCTGTTG AGAATCCGTG GGATGGTGAA 
CTGATTCTCA AACTTCTCTC CGGGCTTCCT CAGCCCTTGC ACTCCCATCC AGACGTTTTT 
GAGTGGCAGT GCAAGCTCCC TGCCATGAAG CCCAAGACCG AGTTCCAGCT GGGTTCTTTG 
CTGGTCTACA TTGATCACCT TCTCGGAGAA GGGGCTTTTG CCCAAGTTTA TGAAGTTACC 
CATGGGGATG TGAAGGATAC CAAAAGCAAA CAGAAATTTG TTCTGAAGGT CCAGAAGCCC 
GCCAACCCCT GGGAGTTCTA CATTGGGACT CAGCTGATGG AGAGGCTGAA GCCCAGTGTG 
CGACACATGT TTATCAGGTT CTACTCCGCC CACTTCTTCC GGGATGGCAG CGTGCTGGTG 
GGGGATCTTC ACAGCTACGG GACGCTGCTG AATGCCATCA ACCTCTATAA AAATACCCCT 
GAGAAAGTGA TGCCTCAAGC TCTGGTGCTG TCCTTTGCTA TCAGCATTCT CCACATGATA 
GAACAGGTCC ACGGCTGCGA GATCATCCAT GGGGATGTCA AACCAGACAA CTTCATACTC 
GGGAGCAGAG TTTTGGAACA AGACAGCGAA GATGATGGTT TATCTGCCGG CCTGGCACTG 
ATTGACCTGG GTCAGAGCAT AGACATGAAA CTTTTCCCAA AAGGAACTAC ATTCACAGGA 
AAGTGTGAAA CGTCCGGTTT CCAGTGTACG GAGATGCTCA GTAACAAACC GTGGACCTAC 
CAGATTGATT ACTTTGGAGT TGCAGCAACA GTGTACTGCA TGCTTTTTGG CTCTTACATG 
AAAGTGAAAA ACGAAGGCGG AGTCTGGAAG CCTGAAGGTC TTTTTAGAAG GCTTCCTCAT 
CTGGACATGT GGAATGAATT TTTTCACATC ATGTTGAATA TACCAGATTG TCATCATCTT 
CCATCTTTGG ATTTGTTAAG GCAAAAGCTG AAGAAAATAT TTCAGCAGCA CTATACAAAC 
AAGATTAAGA CTCTACGTAA TAGGCTAATT GTTCTGCTCT TAGAATATAA GCGTTCTCGA 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011376153.1
CDS	129..3314
Translation	MDDPENAFQMFEAHMQRYKGDDPLGEWESYMQWVEENFPENKEYSTTLLEQLMKEFLDKKRYHDDPRFINYCLKFAECNSDRHQFFEFLYNHGIGARCSHLYIAWAAHLEASGELQLAGAVFRRGLQSRAEPGEPLRQQYRLFQTRVTETQLPAQARTSEPLHDAQILNQMMTSKPNPGNNAACVSKLQGSEASGVTSSACDKESNMEQRVVMISKSGYSAQPSLAARADIQQMVMYCKAKLIRGDSEFSFEELRAQKYNQRRKHEQWVNEDRHYMKRKEANAFEEQLLKEKMDELHKKLHQVAEPPQESLPAFQESPEVDPLCMGPSVGSQQEPSTPSLPAVTQQTSEHLGQRPREAPPAVPPEANAITVTLVSPAVSQSMIPAPLTSQPVTDSMFAAAGKDARCVTRSTHGFKPPSGAELTGGCDTHKVVNTGSFHTTPNTSLGVVQATPSTGQPSPTVHTKEALGFIMNVFQAPTLPDVSDDQDEWPSLDQNEDAFEAQFQKNASSSGAWGVNKVISSLSSAFSVFEDGNKENYGLPQPKNKPAGARTFGERSVSRFSPKPKEVPCPEEFPDDSTVWGVRCPKMLAPSPRSPGGFTSAARLASTPSHKLLVGPVHALEDKENVVAAQYAHVNLDAHEGNVAQPSEDRKFSPMQEGSLAAAAGGALTREAERGLAACTLTDADLATAAGCTDTGTGLEAEQAQPRSPDLAVENPWDGELILKLLSGLPQPLHSHPDVFEWQCKLPAMKPKTEFQLGSLLVYIDHLLGEGAFAQVYEVTHGDVKDTKSKQKFVLKVQKPANPWEFYIGTQLMERLKPSVRHMFIRFYSAHFFRDGSVLVGDLHSYGTLLNAINLYKNTPEKVMPQALVLSFAISILHMIEQVHGCEIIHGDVKPDNFILGSRVLEQDSEDDGLSAGLALIDLGQSIDMKLFPKGTTFTGKCETSGFQCTEMLSNKPWTYQIDYFGVAATVYCMLFGSYMKVKNEGGVWKPEGLFRRLPHLDMWNEFFHIMLNIPDCHHLPSLDLLRQKLKKIFQQHYTNKIKTLRNRLIVLLLEYKRSRK

Target ORF information:

RefSeq Version	XM_011377851.2
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus BUB1 mitotic checkpoint serine/threonine kinase (BUB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011377851.2

ORF Insert Sequence:

ATGGATGACC CGGAAAACGC CTTCCAAATG TTCGAAGCCC ATATGCAGCG GTACAAGGGC 
GATGACCCAC TTGGTGAATG GGAAAGTTAC ATGCAGTGGG TAGAAGAGAA TTTTCCTGAG 
AATAAAGAAT ACTCGACAAC TCTATTAGAG CAGTTAATGA AGGAGTTTTT AGATAAGAAG 
AGATACCACG ATGACCCAAG ATTCATCAAC TATTGTTTAA AATTTGCTGA GTGCAACAGT 
GACCGTCATC AGTTTTTTGA GTTTCTGTAC AACCATGGGA TTGGGGCACG GTGCTCCCAC 
CTGTATATAG CCTGGGCCGC ACACCTGGAG GCCAGTGGAG AGCTGCAGCT CGCTGGCGCT 
GTCTTCCGGA GGGGCCTGCA GAGCCGGGCG GAGCCAGGCG AGCCACTGCG GCAGCAGTAC 
AGGTTATTTC AGACACGTGT CACTGAAACC CAGTTGCCAG CTCAAGCTAG AACCTCAGAA 
CCTCTGCATG ATGCTCAGAT TTTAAACCAG ATGATGACAT CAAAACCAAA TCCAGGAAAT 
AATGCAGCCT GTGTTTCTAA GCTTCAGGGT TCAGAAGCTT CTGGAGTAAC ATCTTCAGCT 
TGTGATAAAG AGTCGAATAT GGAACAAAGA GTGGTCATGA TTTCTAAATC GGGTTACTCT 
GCACAGCCCT CTCTGGCAGC CAGAGCAGAC ATCCAGCAGA TGGTTATGTA CTGCAAGGCA 
AAGCTCATTC GTGGGGACTC AGAATTCTCC TTTGAAGAGC TGAGAGCCCA GAAGTACAAT 
CAGCGGAGAA AGCATGAGCA GTGGGTAAAT GAAGATAGAC ATTACATGAA AAGGAAAGAA 
GCAAATGCTT TTGAAGAACA GCTATTGAAA GAGAAAATGG ATGAACTTCA TAAGAAGTTG 
CATCAAGTGG CGGAGCCACC ACAGGAGAGC CTGCCTGCTT TCCAGGAGAG TCCTGAGGTT 
GACCCACTGT GCATGGGGCC CAGTGTCGGC TCCCAGCAAG AACCGAGTAC TCCGTCTCTG 
CCGGCTGTCA CCCAGCAGAC TTCAGAGCAC CTGGGACAGA GACCCAGAGA GGCACCTCCA 
GCTGTTCCTC CTGAGGCAAA TGCTATCACT GTCACTTTGG TGTCCCCAGC TGTCAGCCAG 
AGTATGATTC CTGCTCCTTT GACATCACAG CCAGTGACAG ACTCCATGTT TGCAGCAGCC 
GGAAAAGATG CCAGATGTGT GACCAGAAGT ACTCATGGAT TCAAGCCGCC GAGCGGAGCA 
GAGCTGACTG GAGGGTGTGA CACACACAAG GTTGTGAACA CAGGTTCTTT TCACACGACT 
CCAAACACGT CGCTGGGAGT GGTCCAGGCA ACGCCGTCCA CGGGGCAGCC ATCCCCCACC 
GTGCACACGA AGGAAGCCTT AGGCTTCATC ATGAATGTGT TCCAGGCTCC TACACTGCCT 
GATGTGTCTG ATGACCAAGA TGAATGGCCA TCTCTAGATC AAAATGAAGA TGCATTCGAG 
GCCCAGTTTC AAAAAAATGC AAGTTCTTCT GGGGCTTGGG GCGTCAATAA GGTTATCTCT 
TCTTTATCAT CTGCTTTTTC TGTGTTTGAA GATGGAAACA AAGAAAATTA TGGGTTACCC 
CAGCCTAAGA ATAAACCCGC AGGAGCCAGG ACCTTTGGAG AACGCAGTGT AAGCAGATTT 
TCCCCTAAAC CAAAGGAAGT GCCATGTCCT GAGGAGTTTC CTGATGACTC GACTGTCTGG 
GGCGTCCGCT GCCCCAAAAT GCTGGCGCCC AGCCCCAGGA GCCCAGGAGG CTTTACATCC 
GCCGCACGGC TTGCATCCAC GCCTTCCCAT AAGCTTCTGG TGGGCCCAGT GCATGCTCTA 
GAGGACAAAG AAAATGTGGT AGCAGCACAG TACGCCCATG TGAATTTGGA TGCTCATGAA 
GGAAACGTGG CGCAACCCTC AGAAGACAGG AAGTTCAGCC CAATGCAGGA GGGAAGCCTA 
GCAGCAGCTG CAGGCGGCGC GCTGACCAGA GAGGCAGAGC GGGGCCTGGC TGCCTGCACC 
CTCACAGACG CTGACCTTGC CACGGCGGCG GGGTGCACAG ACACCGGCAC TGGACTTGAG 
GCAGAGCAGG CACAGCCTCG TTCACCAGAC CTTGCTGTTG AGAATCCGTG GGATGGTGAA 
CTGATTCTCA AACTTCTCTC CGGGCTTCCT CAGCCCTTGC ACTCCCATCC AGACGTTTTT 
GAGTGGCAGT GCAAGCTCCC TGCCATGAAG CCCAAGACCG AGTTCCAGCT GGGTTCTTTG 
CTGGTCTACA TTGATCACCT TCTCGGAGAA GGGGCTTTTG CCCAAGTTTA TGAAGTTACC 
CATGGGGATG TGAAGGATAC CAAAAGCAAA CAGAAATTTG TTCTGAAGGT CCAGAAGCCC 
GCCAACCCCT GGGAGTTCTA CATTGGGACT CAGCTGATGG AGAGGCTGAA GCCCAGTGTG 
CGACACATGT TTATCAGGTT CTACTCCGCC CACTTCTTCC GGGATGGCAG CGTGCTGGTG 
GGGGATCTTC ACAGCTACGG GACGCTGCTG AATGCCATCA ACCTCTATAA AAATACCCCT 
GAGAAAGTGA TGCCTCAAGC TCTGGTGCTG TCCTTTGCTA TCAGCATTCT CCACATGATA 
GAACAGGTCC ACGGCTGCGA GATCATCCAT GGGGATGTCA AACCAGACAA CTTCATACTC 
GGGAGCAGAG TTTTGGAACA AGACAGCGAA GATGATGGTT TATCTGCCGG CCTGGCACTG 
ATTGACCTGG GTCAGAGCAT AGACATGAAA CTTTTCCCAA AAGGAACTAC ATTCACAGGA 
AAGTGTGAAA CGTCCGGTTT CCAGTGTACG GAGATGCTCA GTAACAAACC GTGGACCTAC 
CAGATTGATT ACTTTGGAGT TGCAGCAACA GTGTACTGCA TGCTTTTTGG CTCTTACATG 
AAAGTGAAAA ACGAAGGCGG AGTCTGGAAG CCTGAAGGTC TTTTTAGAAG GCTTCCTCAT 
CTGGACATGT GGAATGAATT TTTTCACATC ATGTTGAATA TACCAGATTG TCATCATCTT 
CCATCTTTGG ATTTGTTAAG GCAAAAGCTG AAGAAAATAT TTCAGCAGCA CTATACAAAC 
AAGATTAAGA CTCTACGTAA TAGGCTAATT GTTCTGCTCT TAGAATATAA GCGTTCTCGA 
AAGTAA