GCLC cDNA ORF clone, Propithecus coquereli(Coquerel's sifaka)

Gene
Clones
gRNAs

Summary

Gene Symbol	GCLC
Entrez Gene ID	105813804
Full Name	glutamate-cysteine ligase catalytic subunit
Gene Type	protein-coding
Organism	Propithecus coquereli(Coquerel's sifaka)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012648602.1	XP_012504056.1	glutamate--cysteine ligase catalytic subunit

Pathways from BioSystems
Gene Ontology

The following GCLC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GCLC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPr107083	XM_012648602.1 Latest version!	Propithecus coquereli glutamate-cysteine ligase, catalytic subunit (GCLC), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GCLC ( XM_012648602.1 ) cDNA ORF clone, Propithecus coquereli(Coquerel's sifaka) -> XP_012504056.1 Propithecus coquereli glutamate-cysteine ligase, catalytic subunit (GCLC), mRNA.

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CloneID	OPr107083
Clone ID Related Accession (Same CDS sequence)	XM_012648602.1
Accession Version	XM_012648602.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-31
Organism	Propithecus coquereli(Coquerel's sifaka)
Product	glutamate--cysteine ligase catalytic subunit
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012148507.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Propithecus coquereli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGC TGTCCCAGGG GTCGCCGCTG AGCTGGGAGG AGACCAAGCG CCACGCCGAC 
CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
CACAAGGACG TTCTCAAGTG GGGCGATGAG GTGGAATACA TGTTGGTATC TTTTGATCAT 
GAAAATAAAA AAGTCCAATT GGTCCTGTCT GGAGAGGAAG TTCTTGAAAC TCTACAAGAG 
AAGGGGGAAA GGACAAACCC AAACCATCCT ACCCTCTGGA GACCAGAGTA TGGGAGTTAC 
ATGATTGAAG GGACACCTGG ACAGCCCTAC GGGGGAACAA TGTCGGAATT CAACACAGTC 
GAAGCCAACA TGAGAAAACG CCGGAAGGAG GCCACTTCTC TATTAGAAGA AAATCAGGCT 
CTTTGCACAA TAACTTCATT TCCAAGATTA GGCTGTCCTG GGTTCACAAT ACCCGAGTAC 
ACACCCAACC CGGTTGAAGG AGGAGCTTCC AAGTCCCTCT TCTTCCCAGA TGAAGCAATA 
AACAAGCACC CTCGCTTCAG TACCTTAACA AGAAATATCC GACACAGGAG AGGAGAAAAG 
GTTGTCATCA ATGTGCCAAT ATTTAAGGAC AAGAACACAC CATCTCCATT TATAGAAACA 
TTTCCTGAGG ATGATGAGGC AGCAAAAGCT TCTAAGCCGG ATCATATTTA CATGGATGCC 
ATGGGATTTG GGATGGGCAA TTGCTGTCTT CAGGTGACAT TCCAAGCCTG CAGCATATCT 
GAGGCCAGAT ACCTTTATGA TCAGTTGGCC ACTATCTGTC CAATTGTCAT GGCTTTGAGT 
GCTGCGTCTC CTTTTTACCG AGGCTACGTG TCAGACATTG ATTGTCGCTG GGGAGTGATT 
TCTGCATCTG TAGATGACAG AACTCGGGAG GAGAGAGGAC TGGAGCCACT GAAGAACAAT 
AACTTTAGGA TTAGTAAATC CCGATACGAC TCAATAGACA GCTACTTATC TAAGTGTGGA 
GAGAAATATA ATGACATCGA CTTGACCATC GATAAAGAAA TCTACGAACA GCTGTTGCAG 
GAAGGCATTG ACCATCTCCT GGCCCAGCAT GTTGCTCACC TCTTTATCAG AGACCCACTG 
ACTCTGTTTG AAGAGAAGAT ACACCTGGAC GATGCCAATG AGTCTGACCA TTTTGAGAAT 
ATTCAGTCCA CAAATTGGCA GACAATGAGA TTTAAGCCCC CTCCTCCAAA CTCAGATATT 
GGATGGAGAG TCGAATTCCG ACCCATGGAG GTTCAGTTAA CAGACTTTGA GAACTCTGCC 
TATGTGGTGT TTGTGGTACT GCTCACCAGA GTGATCCTTT CGTACAAACT GGATTTTCTC 
ATTCCTCTGT CAAAGGTTGA TGAAAACATG AAAGTAGCAC AGAAACGAGA TGCTGTCTTG 
CGGGACATGT TTTACTTCAG GAAAGACATT TGCAAAGGTG GCAATGCTGT GGTGGACGGG 
TGTGGCAAGG CCCAGCACAG CACGGAGCCC GCTGTGGAGG AGTACACCCT CATGAGCATA 
GACACCATCA TCAACGGGAA GGAAGGTGTG TTTCCCGGGC TGATCCCAAT TCTGAACTCT 
TACCTTGAAA ACATGGAAGT GGATGTGGAC ACGAGATGTA GTATTCTCAA CTACCTAAAG 
CTGATTAAGA AGAGAGCATC TGGAGAACTA ATGACTGTTG CCAGATGGAT GAGGGAGTTT 
ATCGCAAACC ATCCTGACTA CAAGCAAGAC AGTGTAATAA CTGATGAAAT GAATTATAGC 
CTTCTTTTGA AGTGTAACCA AATTGCAAAT GAATTATGTG AATGCCCAGA GTTACTCGGA 
TCAGCATTTA GGAAAGTAAA ATACAGTGGA AGTAAAACTG AGTCTTCCAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012504056.1
CDS	418..2331
Translation	MGLLSQGSPLSWEETKRHADHVRRHGILQFLHIYHAVKDRHKDVLKWGDEVEYMLVSFDHENKKVQLVLSGEEVLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEANMRKRRKEATSLLEENQALCTITSFPRLGCPGFTIPEYTPNPVEGGASKSLFFPDEAINKHPRFSTLTRNIRHRRGEKVVINVPIFKDKNTPSPFIETFPEDDEAAKASKPDHIYMDAMGFGMGNCCLQVTFQACSISEARYLYDQLATICPIVMALSAASPFYRGYVSDIDCRWGVISASVDDRTREERGLEPLKNNNFRISKSRYDSIDSYLSKCGEKYNDIDLTIDKEIYEQLLQEGIDHLLAQHVAHLFIRDPLTLFEEKIHLDDANESDHFENIQSTNWQTMRFKPPPPNSDIGWRVEFRPMEVQLTDFENSAYVVFVVLLTRVILSYKLDFLIPLSKVDENMKVAQKRDAVLRDMFYFRKDICKGGNAVVDGCGKAQHSTEPAVEEYTLMSIDTIINGKEGVFPGLIPILNSYLENMEVDVDTRCSILNYLKLIKKRASGELMTVARWMREFIANHPDYKQDSVITDEMNYSLLLKCNQIANELCECPELLGSAFRKVKYSGSKTESSN

Target ORF information:

RefSeq Version	XM_012648602.1
Organism	Propithecus coquereli(Coquerel's sifaka)
Definition	Propithecus coquereli glutamate-cysteine ligase, catalytic subunit (GCLC), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012648602.1

ORF Insert Sequence:

ATGGGGCTGC TGTCCCAGGG GTCGCCGCTG AGCTGGGAGG AGACCAAGCG CCACGCCGAC 
CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
CACAAGGACG TTCTCAAGTG GGGCGATGAG GTGGAATACA TGTTGGTATC TTTTGATCAT 
GAAAATAAAA AAGTCCAATT GGTCCTGTCT GGAGAGGAAG TTCTTGAAAC TCTACAAGAG 
AAGGGGGAAA GGACAAACCC AAACCATCCT ACCCTCTGGA GACCAGAGTA TGGGAGTTAC 
ATGATTGAAG GGACACCTGG ACAGCCCTAC GGGGGAACAA TGTCGGAATT CAACACAGTC 
GAAGCCAACA TGAGAAAACG CCGGAAGGAG GCCACTTCTC TATTAGAAGA AAATCAGGCT 
CTTTGCACAA TAACTTCATT TCCAAGATTA GGCTGTCCTG GGTTCACAAT ACCCGAGTAC 
ACACCCAACC CGGTTGAAGG AGGAGCTTCC AAGTCCCTCT TCTTCCCAGA TGAAGCAATA 
AACAAGCACC CTCGCTTCAG TACCTTAACA AGAAATATCC GACACAGGAG AGGAGAAAAG 
GTTGTCATCA ATGTGCCAAT ATTTAAGGAC AAGAACACAC CATCTCCATT TATAGAAACA 
TTTCCTGAGG ATGATGAGGC AGCAAAAGCT TCTAAGCCGG ATCATATTTA CATGGATGCC 
ATGGGATTTG GGATGGGCAA TTGCTGTCTT CAGGTGACAT TCCAAGCCTG CAGCATATCT 
GAGGCCAGAT ACCTTTATGA TCAGTTGGCC ACTATCTGTC CAATTGTCAT GGCTTTGAGT 
GCTGCGTCTC CTTTTTACCG AGGCTACGTG TCAGACATTG ATTGTCGCTG GGGAGTGATT 
TCTGCATCTG TAGATGACAG AACTCGGGAG GAGAGAGGAC TGGAGCCACT GAAGAACAAT 
AACTTTAGGA TTAGTAAATC CCGATACGAC TCAATAGACA GCTACTTATC TAAGTGTGGA 
GAGAAATATA ATGACATCGA CTTGACCATC GATAAAGAAA TCTACGAACA GCTGTTGCAG 
GAAGGCATTG ACCATCTCCT GGCCCAGCAT GTTGCTCACC TCTTTATCAG AGACCCACTG 
ACTCTGTTTG AAGAGAAGAT ACACCTGGAC GATGCCAATG AGTCTGACCA TTTTGAGAAT 
ATTCAGTCCA CAAATTGGCA GACAATGAGA TTTAAGCCCC CTCCTCCAAA CTCAGATATT 
GGATGGAGAG TCGAATTCCG ACCCATGGAG GTTCAGTTAA CAGACTTTGA GAACTCTGCC 
TATGTGGTGT TTGTGGTACT GCTCACCAGA GTGATCCTTT CGTACAAACT GGATTTTCTC 
ATTCCTCTGT CAAAGGTTGA TGAAAACATG AAAGTAGCAC AGAAACGAGA TGCTGTCTTG 
CGGGACATGT TTTACTTCAG GAAAGACATT TGCAAAGGTG GCAATGCTGT GGTGGACGGG 
TGTGGCAAGG CCCAGCACAG CACGGAGCCC GCTGTGGAGG AGTACACCCT CATGAGCATA 
GACACCATCA TCAACGGGAA GGAAGGTGTG TTTCCCGGGC TGATCCCAAT TCTGAACTCT 
TACCTTGAAA ACATGGAAGT GGATGTGGAC ACGAGATGTA GTATTCTCAA CTACCTAAAG 
CTGATTAAGA AGAGAGCATC TGGAGAACTA ATGACTGTTG CCAGATGGAT GAGGGAGTTT 
ATCGCAAACC ATCCTGACTA CAAGCAAGAC AGTGTAATAA CTGATGAAAT GAATTATAGC 
CTTCTTTTGA AGTGTAACCA AATTGCAAAT GAATTATGTG AATGCCCAGA GTTACTCGGA 
TCAGCATTTA GGAAAGTAAA ATACAGTGGA AGTAAAACTG AGTCTTCCAA CTAG