SRD5A2 cDNA ORF clone, Pongo abelii(Sumatran orangutan)

Gene
Clones
gRNAs

Summary

Gene Symbol	SRD5A2
Entrez Gene ID	100454013
Full Name	3-oxo-5-alpha-steroid 4-dehydrogenase 2
Synonyms	SRD5A2
Gene Type	protein-coding
Organism	Pongo abelii(Sumatran orangutan)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003780297.1	XP_003780345.1	LOW QUALITY PROTEIN: 3-oxo-5-alpha-steroid 4-dehydrogenase 2
XM_024242570.1	XP_024098338.1	3-oxo-5-alpha-steroid 4-dehydrogenase 2

Pathways from BioSystems
Gene Ontology

The following SRD5A2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SRD5A2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSa19610	XM_003780297.1 Latest version!	Pongo abelii 3-oxo-5-alpha-steroid 4-dehydrogenase 2 (LOC100454013), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OSa177774	XM_024242570.1 Latest version!	Pongo abelii steroid 5 alpha-reductase 2 (SRD5A2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SRD5A2 ( XM_003780297.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_003780345.1 Pongo abelii 3-oxo-5-alpha-steroid 4-dehydrogenase 2 (LOC100454013), mRNA.

Price & Availability↑

CloneID	OSa19610
Clone ID Related Accession (Same CDS sequence)	XM_003780297.1
Accession Version	XM_003780297.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-09-22
Organism	Pongo abelii(Sumatran orangutan)
Product	LOW QUALITY PROTEIN: 3-oxo-5-alpha-steroid 4-dehydrogenase 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002876218.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pongo abelii Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 9% of CDS bases frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGTTCTTCT TTAACAGTGG ATCCCAACCT TTCCTCCCTG TGTGTCCCAG GACATTTGTG 
TACTCACTGC TCAATCGAGG GAGGCCTTAT CCAGCTATAC TCATTCTCAG AGGCATTGCC 
TTCTGCACTG GAAATGGAGT CCTTCAAGGC TACTATCTGA TTTATTGTGC TGAATACCCT 
GATGGGTGGT ACACAGACAT ACGGGGTGTC TTCTTATTTA TTTTGGGAAT GGGAATCAAC 
ATTCATAGTG ACTATATATT GCGCCAGCTC AGGAAGCCTG GAGAAATCAG CTACAGGATT 
CCACAAGGTG GCTTGTTTAC GTATGTTTCT GGAGCCAATT TCCTCGGTGA GATCATTGAA 
TGGATCGGCT ATGCCCTGGC CACTTGGTCC CTCCCAGCAA CTTGCCTTTT GCATTTTTCT 
CACTTTGTTT CCTTGGGCCT GCGAGCTTTT CACCACCATA GGTTCTACCT CAAGATGTTT 
GAGGACTACC CCAAATCTCG GAAAGCCCTT ATTCCATTCA TCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003780345.1
CDS	1..528
Translation	MFFFNSGSQPFLPVCPRTFVYSLLNRGRPYPAILILRGIAFCTGNGVLQGYYLIYCAEYPDGWYTDIRGVFLFILGMGINIHSDYILRQLRKPGEISYRIPQGGLFTYVSGANFLGEIIEWIGYALATWSLPATCLLHFSHFVSLGLRAFHHHRFYLKMFEDYPKSRKALIPFIF

Target ORF information:

RefSeq Version	XM_003780297.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii 3-oxo-5-alpha-steroid 4-dehydrogenase 2 (LOC100454013), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003780297.1

ORF Insert Sequence:

ATGTTCTTCT TTAACAGTGG ATCCCAACCT TTCCTCCCTG TGTGTCCCAG GACATTTGTG 
TACTCACTGC TCAATCGAGG GAGGCCTTAT CCAGCTATAC TCATTCTCAG AGGCATTGCC 
TTCTGCACTG GAAATGGAGT CCTTCAAGGC TACTATCTGA TTTATTGTGC TGAATACCCT 
GATGGGTGGT ACACAGACAT ACGGGGTGTC TTCTTATTTA TTTTGGGAAT GGGAATCAAC 
ATTCATAGTG ACTATATATT GCGCCAGCTC AGGAAGCCTG GAGAAATCAG CTACAGGATT 
CCACAAGGTG GCTTGTTTAC GTATGTTTCT GGAGCCAATT TCCTCGGTGA GATCATTGAA 
TGGATCGGCT ATGCCCTGGC CACTTGGTCC CTCCCAGCAA CTTGCCTTTT GCATTTTTCT 
CACTTTGTTT CCTTGGGCCT GCGAGCTTTT CACCACCATA GGTTCTACCT CAAGATGTTT 
GAGGACTACC CCAAATCTCG GAAAGCCCTT ATTCCATTCA TCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SRD5A2 ( XM_024242570.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024098338.1 Pongo abelii steroid 5 alpha-reductase 2 (SRD5A2), mRNA.

Price & Availability↑

CloneID	OSa177774
Clone ID Related Accession (Same CDS sequence)	XM_024242570.1
Accession Version	XM_024242570.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	3-oxo-5-alpha-steroid 4-dehydrogenase 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036904.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGTTC AGTGCCAGCA GAGCCCAGTG CTGGCAGGCA GCGCCACTTT GGTCGCCCTT 
GGGGCACTGG CCTTGTACGT CGCGAAGCCC TCCGGCTACG GGAAGCACAC GGAGAGCCTG 
ATGCCGGCGG CTACCCGCCT GCCAGCCCGC GCCGCCTGGT TCCTGCAGGA GCTGCCCTCC 
TTCGCGGTGC CCGCGGGGAT CCTCGCCTGG CAGCCCCTCT CCCTCTTCGG GCCACCTGGG 
ACGGTGCTTC TGGGCCTCTT CTGCGTACAT TACTTCCACA GGACATTTGT GTACTCACTG 
CTCAGTCGAG GGAGGCCTTA TCCAGCTATA CTCATTCTCA GAGGCATTGC CTTCTGCACT 
GGAAATGGAG TCCTTCAAGG CTACTATCTG ATTTATTGTG CTGAATACCC TGATGGGTGG 
TACACAGACA TACGGTTTAG CTTGGGTGTC TTCTTATTTA TTTTGGGAAT GGGAATCAAC 
ATTCATAGTG ACTATATATT GCGCCAGCTC AGGAAGCCTG GAGAAATCAG CTACAGGATT 
CCACAAGGTG GCTTGTTTAC GTATGTTTCT GGAGCCAATT TCCTCGGTGA GATCATTGAA 
TGGATCGGCT ATGCCCTGGC CACTTGGTCC CTCCCAGCAC TTGCCTTTGC ATTTTTCTCA 
CTTTGTTTCC TTGGGCTGCG AGCTTTTCAC CACCATAGGT TCTACCTCAA GATGTTCGAG 
GACTACCCCA AATCTCGGAA AGCCCTTATT CCATTCATCT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024098338.1
CDS	66..830
Translation	MQVQCQQSPVLAGSATLVALGALALYVAKPSGYGKHTESLMPAATRLPARAAWFLQELPSFAVPAGILAWQPLSLFGPPGTVLLGLFCVHYFHRTFVYSLLSRGRPYPAILILRGIAFCTGNGVLQGYYLIYCAEYPDGWYTDIRFSLGVFLFILGMGINIHSDYILRQLRKPGEISYRIPQGGLFTYVSGANFLGEIIEWIGYALATWSLPALAFAFFSLCFLGLRAFHHHRFYLKMFEDYPKSRKALIPFIF

Target ORF information:

RefSeq Version	XM_024242570.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii steroid 5 alpha-reductase 2 (SRD5A2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024242570.1

ORF Insert Sequence:

ATGCAGGTTC AGTGCCAGCA GAGCCCAGTG CTGGCAGGCA GCGCCACTTT GGTCGCCCTT 
GGGGCACTGG CCTTGTACGT CGCGAAGCCC TCCGGCTACG GGAAGCACAC GGAGAGCCTG 
ATGCCGGCGG CTACCCGCCT GCCAGCCCGC GCCGCCTGGT TCCTGCAGGA GCTGCCCTCC 
TTCGCGGTGC CCGCGGGGAT CCTCGCCTGG CAGCCCCTCT CCCTCTTCGG GCCACCTGGG 
ACGGTGCTTC TGGGCCTCTT CTGCGTACAT TACTTCCACA GGACATTTGT GTACTCACTG 
CTCAGTCGAG GGAGGCCTTA TCCAGCTATA CTCATTCTCA GAGGCATTGC CTTCTGCACT 
GGAAATGGAG TCCTTCAAGG CTACTATCTG ATTTATTGTG CTGAATACCC TGATGGGTGG 
TACACAGACA TACGGTTTAG CTTGGGTGTC TTCTTATTTA TTTTGGGAAT GGGAATCAAC 
ATTCATAGTG ACTATATATT GCGCCAGCTC AGGAAGCCTG GAGAAATCAG CTACAGGATT 
CCACAAGGTG GCTTGTTTAC GTATGTTTCT GGAGCCAATT TCCTCGGTGA GATCATTGAA 
TGGATCGGCT ATGCCCTGGC CACTTGGTCC CTCCCAGCAC TTGCCTTTGC ATTTTTCTCA 
CTTTGTTTCC TTGGGCTGCG AGCTTTTCAC CACCATAGGT TCTACCTCAA GATGTTCGAG 
GACTACCCCA AATCTCGGAA AGCCCTTATT CCATTCATCT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.