PCK1 cDNA ORF clone, Pongo abelii(Sumatran orangutan)

Gene
Clones
gRNAs

Summary

Gene Symbol	PCK1
Entrez Gene ID	100173760
Full Name	phosphoenolpyruvate carboxykinase 1
Synonyms	PPCK1
Gene Type	protein-coding
Organism	Pongo abelii(Sumatran orangutan)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001133286.1	NP_001126758.1	phosphoenolpyruvate carboxykinase, cytosolic [GTP]

Pathways from BioSystems
Gene Ontology

The following PCK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PCK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSa20647	NM_001133286.1 Latest version!	Pongo abelii phosphoenolpyruvate carboxykinase 1 (PCK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PCK1 ( NM_001133286.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> NP_001126758.1 Pongo abelii phosphoenolpyruvate carboxykinase 1 (PCK1), mRNA.

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CloneID	OSa20647
Clone ID Related Accession (Same CDS sequence)	NM_001133286.1
Accession Version	NM_001133286.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Pongo abelii(Sumatran orangutan)
Product	phosphoenolpyruvate carboxykinase, cytosolic [GTP]
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR860867.1. ##Evidence-Data-START## Transcript exon combination :: CR860867.1 [ECO:0000332] ##Evidence-Data-END##

ATGCCTCCTC AGCTGCAAAA CGGCCTGAAC CTCTCGGCCA AAGTTGTCCA GGGAAGCCTG 
GACAGCCTGC CCCAGGCAGT GAGGAAGTTT CTCGAGAATA ACGCTGAGCT GTGTCAGCCT 
GATCACATCC ACATCTGCGA CGGCTCTGAG GAGGAGAATG GGCGGCTTCT GGGCCAGATG 
GAGGAGGAGG GCATCCTCAG GCGGCTGAAG AAGTATGACA ACTGCTGGTT GGCTCTCACT 
GACCCCAGGG ATGTGGCCAG GATCGGAAGC AAGACGGTTA TCGTCACCCA AGAGCAAAGA 
GACACAGTGC CCATCCCCAA AACAGGCCTC AGCCAGCTCG GTCGCTGGAT GTCAGAGGAG 
GATTTTGAGA AAGCGCTCAA TGCCAGGTTC CCAGGGTGCA TGAAAGGTCG CACCATGTAT 
GTCATCCCAT TCAGCATGGG GCCGCTGGGC TCGCCTCTGT CGAAGATCGG CATCGAGCTG 
ACAGATTCAC CCTACGTGGT GGCCAGCATG CGGATCATGA CGCGGATGGG CACGCCCGTC 
CTGGAAGCAC TGGGCGATGG GGAGTTTGTC AAATGCCTCC ATTCTGTGGG GTGCCCTCTG 
CCTTTACAAA AGCCTTTGGT CAACAACTGG CCCTGCAACC CGGAGCTGAC GCTCATCGCC 
CACCTGCCTG ACCGCAGAGA GATCATCTCC TTTGGCAGTG GGTATGGCGG GAACTCGCTG 
CTCGGGAAGA AGTGCTTTGC TCTCAGGATG GCCAGCCGGC TGGCCAAGGA GGAAGGATGG 
CTGGCAGAGC ACATGCTGAT TCTGGGTATA ACCAACCCTG AGGGTGAGAA GAAGTACCTG 
GCGGCCGCAT TTCCCAGCGC CTGCGGGAAG ACCAACCTGG CCATGATGAA CCCCAGCCTC 
CCCGGGTGGA AGGTTGAGTG CGTCGGGGAT GACATTGCCT GGATGAAGTT TGACGCACAA 
GGTCATTTAA GGGCTATCAA CCCAGAAAAT GGCTTTTTCG GTGTCGCTCC TGGGACTTCA 
GTGAAGACCA ACCCCAATGC CATCAAGACC ATCCAGAAGA ACACCATCTT TACCAATGTG 
GCCGAGACCA GCGACGGGGG CGTTTACTGG GAAGGCATTG ATGAGCCGCT AGCTTCAGGC 
GTCACCATCA CTTCCTGGAA GAATAAGGAG TGGAGCCCAG AGGATGGGGA ACCTTGTGCC 
CACCCCAACT CCAGGTTCTG CACCCCAGCC AGCCAGTGCC CCATCATTGA TGCTGCCTGG 
GAGTCTCCGG AAGGTGTTCC CATTGAAGGC ATTATCTTTG GAGGCCGTAG ACCTGCTGGT 
GTCCCTCTAG TCTATGAAGC TCTCAGCTGG CAACATGGAG TCTTTGTGGG GGCGGCCATG 
AGATCAGAGG CCACAGCAGC TGCAGAACAT AAAGGCAAAA TCATCATGCA TGACCCCTTT 
GCCATGCGGC CCTTCTTTGG CTACAACTTT GGCAAATACC TGGCCCACTG GCTTAGCATG 
GCCCAGCACC CAGCAGCCAA ACTGCCCAAG ATCTTCCATG TCAACTGGTT CCGGAAGGAC 
AAGGAAGGCA AATTCCTCTG GCCAGGCTTT GGAGAGAACT CCAGGGTGCT GGAGTGGATG 
TTCAACCGCA TCGATGGGAA AGCCGGTGCC AAGCTCACAC CCATAGGCTA CATCCCCAAG 
GAGGATGCCC TGAATCTGAA AGGCCTGGGG CACATCAACG TGATGGAGCT CTTCAGCATC 
TCCAAGGAAT TCTGGGAGAA GGAGGTGGAA GACATCGAGA AGTATCTGGA GGATCAGGTC 
AATGCTGACC TCCCCTGTGA AATTGAGAGA GAGATCCTTG CCTTGAAGCA AAGAATAAGC 
CAGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001126758.1
CDS	152..2020
Translation	MPPQLQNGLNLSAKVVQGSLDSLPQAVRKFLENNAELCQPDHIHICDGSEEENGRLLGQMEEEGILRRLKKYDNCWLALTDPRDVARIGSKTVIVTQEQRDTVPIPKTGLSQLGRWMSEEDFEKALNARFPGCMKGRTMYVIPFSMGPLGSPLSKIGIELTDSPYVVASMRIMTRMGTPVLEALGDGEFVKCLHSVGCPLPLQKPLVNNWPCNPELTLIAHLPDRREIISFGSGYGGNSLLGKKCFALRMASRLAKEEGWLAEHMLILGITNPEGEKKYLAAAFPSACGKTNLAMMNPSLPGWKVECVGDDIAWMKFDAQGHLRAINPENGFFGVAPGTSVKTNPNAIKTIQKNTIFTNVAETSDGGVYWEGIDEPLASGVTITSWKNKEWSPEDGEPCAHPNSRFCTPASQCPIIDAAWESPEGVPIEGIIFGGRRPAGVPLVYEALSWQHGVFVGAAMRSEATAAAEHKGKIIMHDPFAMRPFFGYNFGKYLAHWLSMAQHPAAKLPKIFHVNWFRKDKEGKFLWPGFGENSRVLEWMFNRIDGKAGAKLTPIGYIPKEDALNLKGLGHINVMELFSISKEFWEKEVEDIEKYLEDQVNADLPCEIEREILALKQRISQM

Target ORF information:

RefSeq Version	NM_001133286.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii phosphoenolpyruvate carboxykinase 1 (PCK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001133286.1

ORF Insert Sequence:

ATGCCTCCTC AGCTGCAAAA CGGCCTGAAC CTCTCGGCCA AAGTTGTCCA GGGAAGCCTG 
GACAGCCTGC CCCAGGCAGT GAGGAAGTTT CTCGAGAATA ACGCTGAGCT GTGTCAGCCT 
GATCACATCC ACATCTGCGA CGGCTCTGAG GAGGAGAATG GGCGGCTTCT GGGCCAGATG 
GAGGAGGAGG GCATCCTCAG GCGGCTGAAG AAGTATGACA ACTGCTGGTT GGCTCTCACT 
GACCCCAGGG ATGTGGCCAG GATCGGAAGC AAGACGGTTA TCGTCACCCA AGAGCAAAGA 
GACACAGTGC CCATCCCCAA AACAGGCCTC AGCCAGCTCG GTCGCTGGAT GTCAGAGGAG 
GATTTTGAGA AAGCGCTCAA TGCCAGGTTC CCAGGGTGCA TGAAAGGTCG CACCATGTAT 
GTCATCCCAT TCAGCATGGG GCCGCTGGGC TCGCCTCTGT CGAAGATCGG CATCGAGCTG 
ACAGATTCAC CCTACGTGGT GGCCAGCATG CGGATCATGA CGCGGATGGG CACGCCCGTC 
CTGGAAGCAC TGGGCGATGG GGAGTTTGTC AAATGCCTCC ATTCTGTGGG GTGCCCTCTG 
CCTTTACAAA AGCCTTTGGT CAACAACTGG CCCTGCAACC CGGAGCTGAC GCTCATCGCC 
CACCTGCCTG ACCGCAGAGA GATCATCTCC TTTGGCAGTG GGTATGGCGG GAACTCGCTG 
CTCGGGAAGA AGTGCTTTGC TCTCAGGATG GCCAGCCGGC TGGCCAAGGA GGAAGGATGG 
CTGGCAGAGC ACATGCTGAT TCTGGGTATA ACCAACCCTG AGGGTGAGAA GAAGTACCTG 
GCGGCCGCAT TTCCCAGCGC CTGCGGGAAG ACCAACCTGG CCATGATGAA CCCCAGCCTC 
CCCGGGTGGA AGGTTGAGTG CGTCGGGGAT GACATTGCCT GGATGAAGTT TGACGCACAA 
GGTCATTTAA GGGCTATCAA CCCAGAAAAT GGCTTTTTCG GTGTCGCTCC TGGGACTTCA 
GTGAAGACCA ACCCCAATGC CATCAAGACC ATCCAGAAGA ACACCATCTT TACCAATGTG 
GCCGAGACCA GCGACGGGGG CGTTTACTGG GAAGGCATTG ATGAGCCGCT AGCTTCAGGC 
GTCACCATCA CTTCCTGGAA GAATAAGGAG TGGAGCCCAG AGGATGGGGA ACCTTGTGCC 
CACCCCAACT CCAGGTTCTG CACCCCAGCC AGCCAGTGCC CCATCATTGA TGCTGCCTGG 
GAGTCTCCGG AAGGTGTTCC CATTGAAGGC ATTATCTTTG GAGGCCGTAG ACCTGCTGGT 
GTCCCTCTAG TCTATGAAGC TCTCAGCTGG CAACATGGAG TCTTTGTGGG GGCGGCCATG 
AGATCAGAGG CCACAGCAGC TGCAGAACAT AAAGGCAAAA TCATCATGCA TGACCCCTTT 
GCCATGCGGC CCTTCTTTGG CTACAACTTT GGCAAATACC TGGCCCACTG GCTTAGCATG 
GCCCAGCACC CAGCAGCCAA ACTGCCCAAG ATCTTCCATG TCAACTGGTT CCGGAAGGAC 
AAGGAAGGCA AATTCCTCTG GCCAGGCTTT GGAGAGAACT CCAGGGTGCT GGAGTGGATG 
TTCAACCGCA TCGATGGGAA AGCCGGTGCC AAGCTCACAC CCATAGGCTA CATCCCCAAG 
GAGGATGCCC TGAATCTGAA AGGCCTGGGG CACATCAACG TGATGGAGCT CTTCAGCATC 
TCCAAGGAAT TCTGGGAGAA GGAGGTGGAA GACATCGAGA AGTATCTGGA GGATCAGGTC 
AATGCTGACC TCCCCTGTGA AATTGAGAGA GAGATCCTTG CCTTGAAGCA AAGAATAAGC 
CAGATGTAA