ddx21 cDNA ORF clone, Poecilia latipinna(sailfin molly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ddx21
Entrez Gene ID	106939845
Full Name	DExD-box helicase 21
Gene Type	protein-coding
Organism	Poecilia latipinna(sailfin molly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015022407.1	XP_014877893.1	nucleolar RNA helicase 2

Pathways from BioSystems
Gene Ontology

The following ddx21 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ddx21 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo97175	XM_015022407.1 Latest version!	Poecilia latipinna DEAD (Asp-Glu-Ala-Asp) box helicase 21 (ddx21), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ddx21 ( XM_015022407.1 ) cDNA ORF clone, Poecilia latipinna(sailfin molly) -> XP_014877893.1 Poecilia latipinna DEAD (Asp-Glu-Ala-Asp) box helicase 21 (ddx21), mRNA.

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CloneID	OPo97175
Clone ID Related Accession (Same CDS sequence)	XM_015022407.1
Accession Version	XM_015022407.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-14
Organism	Poecilia latipinna(sailfin molly)
Product	nucleolar RNA helicase 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015115614.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia latipinna Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTCAA AAATCATCTT TGAAACTGAA GAAGAAACCA CCGAGAAAGA TATGGACACC 
GTCGCGGAAG CTGAAACCTT CAAGGTGAAG AACAAGACAA AGGAAGGTAA GAAGCTGAAG 
AAGAAACAAC AGCAGTCTGA GGAGCAGGAG GAGGAACCAG ACTGTGAACC TCCAGCACCA 
AAGAAGAAAA AGAAGAAGGA CAAGCTGGCA GCGGACCAAG CGAACGGCCA CATCGAAGAG 
GCCGCTGACC TGAACGGTAA CAGCGGTGAT GTGGAGTCGC CAAAAAAAAT AAAGAAAAAG 
AAAAAGGAAG CATCAGAGAA GAGCCAGAAG CAAAAGAAAG CAACCACTGA GGCAGCAACC 
AATGGACATT CCACCCCAAA CACACCAGTT CAAACTCCCA GTCAGACCCC ATCCCAGTCG 
AGCGATGAGT TGGCCAGTGA CAGCGAAAAT GAGCAGACAC CTGAGCAGAG AGAAGGAGCT 
TTCTCCAACT TCAGAATTTC CAAAGTCACC ATTGATAAAC TGAAAGCTCG GGGAGTCACC 
TACTTGTTTG ACATCCAAGT CAAGTCATTT AATGCTGTAT ATGATGGAGA GGATGTAATC 
GCCCAAGCCC GTACTGGAAC AGGAAAGACC TTCTCCTTCG CCATACCGCT GGTGGAAAAG 
CTCCAGAACG ATTCGGTGGA GATGACGAGA GGCCGGCCTC CCAAGGTGCT AGTGTTGGCT 
CCTACCAGAG AATTGGCTAT CCAGGTCTCC AAGGACTTCA AAGACATCGC CAAAAGACTG 
TCCATCGTCT GTTTCTATGG AGGCAGCTCA TATAACCCAC AGATTGATGC TATTCGTAAC 
GGAATCGATA TTTTGGTGGG AACGCCCGGT CGCATTAAAG ATCACCTTGA GAACAACAAA 
CTCAACCTCT CCAAACTGAA ACACGTCGTT CTGGACGAAG TGGACCAAAT GTTGGACATG 
GGGTTTGCTG AACAAGTTGA AGAGATTTTG GGTTCTTCGT ATAAGAAAGA CAGCGACACC 
AACCCGCAGA CTCTTCTGTT TTCTGCAACC TGCCCTCCCT GGGTGTACGA TGTGGCCAAG 
AAATACATGA GGCCAGGCTG CAAGCACATC GACTTGATCG GCAAGAAAAC ACAAAAAGCA 
GCAACAACTG TTGAGCACCT GGCCATCACG TGCCACTGGT CCCAGCGTGC TGCAGTGATT 
GGAGATGTAA TCCAGGTGTA CAGTGGCAGC CATGGCAGAA CAATCGTCTT CTGCGAGACT 
AAGAAGGAAG CCAATGAACT GGCCATGAAT GCATCCATCA AACAGAGTTC CCAGTCTCTC 
CATGGGGATA TTCCACAGAA ACAGAGAGAG ACGACTCTGA AAGGTTTCAG GAATGGTGTC 
TTTGAGGTCC TTGTTGCCAC AAACGTCGCC GCCCGTGGAT TAGACATCCC AGAGGTCGAC 
TTGGTGGTCC AGTGTTCTCC GCCGAAGGAC GTCGAGTCCT ACATCCATCG TTCAGGGCGA 
ACAGGACGAG CGGGCAGGAC TGGCATCTGC ATCTGCTTCT ACCAGAGGAA GGAAGAGGAC 
CAGTTGCGCT ACGTGGAGAA CAAAGCCGGG ATCACTTTCA GGCGAGTCGG TGTCCCAACT 
GCCAATGACA TCATCAAATC ATCCAGCAAA GACGCCGCCA GGTTTTTGGA CTCTGTTCCA 
GCCACAGCTG TTGAATATTT CAGGGAATCG GCAGAGAAGC TGATCAAAGA GAAGGGAGCG 
GTGGATGCAC TCGCTGCTGC TCTGGCTCAC ATTTCCGGAG CCACGAGTCT GGAGCAGAGA 
TCTTTGCTCA ACTCTGACGC TGGCTACACC ACGATGCAGT TGCAGTGCTC CCAGGAGATG 
CACAATCTGG GTTATGCCTG GAGAACCATT AAAGAGCAGC TGGGAGAGGA CTTTGAAAAC 
CACATTCACA GAATGACCTT CCTCAAAGGC AAAACGGGAG TTTGTTTCGA TGTCCCGGCT 
GATAAAGTCA AAGCGATTCA GGATAATTGG AAAGATGGTC GCCGCTGGCA GCTGACTGTA 
GCCACAGAGC TCCCAGAGCT GGAGGAGAAG CAGTTCCCCA ACCGCGGAGA CAGAGGCGGG 
TTTAGAGGCG GGAGGGGGCG CGGCTTCGGT GGAAACGGAG GACGTAACGG CGGCTTCCGG 
AGCGGCGGTG GCGGCGGCTA CAACAACCGA GGAGGACACA AACGGACCTT CAACCAAGCG 
TTCGATTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014877893.1
CDS	91..2322
Translation	MPSKIIFETEEETTEKDMDTVAEAETFKVKNKTKEGKKLKKKQQQSEEQEEEPDCEPPAPKKKKKKDKLAADQANGHIEEAADLNGNSGDVESPKKIKKKKKEASEKSQKQKKATTEAATNGHSTPNTPVQTPSQTPSQSSDELASDSENEQTPEQREGAFSNFRISKVTIDKLKARGVTYLFDIQVKSFNAVYDGEDVIAQARTGTGKTFSFAIPLVEKLQNDSVEMTRGRPPKVLVLAPTRELAIQVSKDFKDIAKRLSIVCFYGGSSYNPQIDAIRNGIDILVGTPGRIKDHLENNKLNLSKLKHVVLDEVDQMLDMGFAEQVEEILGSSYKKDSDTNPQTLLFSATCPPWVYDVAKKYMRPGCKHIDLIGKKTQKAATTVEHLAITCHWSQRAAVIGDVIQVYSGSHGRTIVFCETKKEANELAMNASIKQSSQSLHGDIPQKQRETTLKGFRNGVFEVLVATNVAARGLDIPEVDLVVQCSPPKDVESYIHRSGRTGRAGRTGICICFYQRKEEDQLRYVENKAGITFRRVGVPTANDIIKSSSKDAARFLDSVPATAVEYFRESAEKLIKEKGAVDALAAALAHISGATSLEQRSLLNSDAGYTTMQLQCSQEMHNLGYAWRTIKEQLGEDFENHIHRMTFLKGKTGVCFDVPADKVKAIQDNWKDGRRWQLTVATELPELEEKQFPNRGDRGGFRGGRGRGFGGNGGRNGGFRSGGGGGYNNRGGHKRTFNQAFDY

Target ORF information:

RefSeq Version	XM_015022407.1
Organism	Poecilia latipinna(sailfin molly)
Definition	Poecilia latipinna DEAD (Asp-Glu-Ala-Asp) box helicase 21 (ddx21), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015022407.1

ORF Insert Sequence:

ATGCCGTCAA AAATCATCTT TGAAACTGAA GAAGAAACCA CCGAGAAAGA TATGGACACC 
GTCGCGGAAG CTGAAACCTT CAAGGTGAAG AACAAGACAA AGGAAGGTAA GAAGCTGAAG 
AAGAAACAAC AGCAGTCTGA GGAGCAGGAG GAGGAACCAG ACTGTGAACC TCCAGCACCA 
AAGAAGAAAA AGAAGAAGGA CAAGCTGGCA GCGGACCAAG CGAACGGCCA CATCGAAGAG 
GCCGCTGACC TGAACGGTAA CAGCGGTGAT GTGGAGTCGC CAAAAAAAAT AAAGAAAAAG 
AAAAAGGAAG CATCAGAGAA GAGCCAGAAG CAAAAGAAAG CAACCACTGA GGCAGCAACC 
AATGGACATT CCACCCCAAA CACACCAGTT CAAACTCCCA GTCAGACCCC ATCCCAGTCG 
AGCGATGAGT TGGCCAGTGA CAGCGAAAAT GAGCAGACAC CTGAGCAGAG AGAAGGAGCT 
TTCTCCAACT TCAGAATTTC CAAAGTCACC ATTGATAAAC TGAAAGCTCG GGGAGTCACC 
TACTTGTTTG ACATCCAAGT CAAGTCATTT AATGCTGTAT ATGATGGAGA GGATGTAATC 
GCCCAAGCCC GTACTGGAAC AGGAAAGACC TTCTCCTTCG CCATACCGCT GGTGGAAAAG 
CTCCAGAACG ATTCGGTGGA GATGACGAGA GGCCGGCCTC CCAAGGTGCT AGTGTTGGCT 
CCTACCAGAG AATTGGCTAT CCAGGTCTCC AAGGACTTCA AAGACATCGC CAAAAGACTG 
TCCATCGTCT GTTTCTATGG AGGCAGCTCA TATAACCCAC AGATTGATGC TATTCGTAAC 
GGAATCGATA TTTTGGTGGG AACGCCCGGT CGCATTAAAG ATCACCTTGA GAACAACAAA 
CTCAACCTCT CCAAACTGAA ACACGTCGTT CTGGACGAAG TGGACCAAAT GTTGGACATG 
GGGTTTGCTG AACAAGTTGA AGAGATTTTG GGTTCTTCGT ATAAGAAAGA CAGCGACACC 
AACCCGCAGA CTCTTCTGTT TTCTGCAACC TGCCCTCCCT GGGTGTACGA TGTGGCCAAG 
AAATACATGA GGCCAGGCTG CAAGCACATC GACTTGATCG GCAAGAAAAC ACAAAAAGCA 
GCAACAACTG TTGAGCACCT GGCCATCACG TGCCACTGGT CCCAGCGTGC TGCAGTGATT 
GGAGATGTAA TCCAGGTGTA CAGTGGCAGC CATGGCAGAA CAATCGTCTT CTGCGAGACT 
AAGAAGGAAG CCAATGAACT GGCCATGAAT GCATCCATCA AACAGAGTTC CCAGTCTCTC 
CATGGGGATA TTCCACAGAA ACAGAGAGAG ACGACTCTGA AAGGTTTCAG GAATGGTGTC 
TTTGAGGTCC TTGTTGCCAC AAACGTCGCC GCCCGTGGAT TAGACATCCC AGAGGTCGAC 
TTGGTGGTCC AGTGTTCTCC GCCGAAGGAC GTCGAGTCCT ACATCCATCG TTCAGGGCGA 
ACAGGACGAG CGGGCAGGAC TGGCATCTGC ATCTGCTTCT ACCAGAGGAA GGAAGAGGAC 
CAGTTGCGCT ACGTGGAGAA CAAAGCCGGG ATCACTTTCA GGCGAGTCGG TGTCCCAACT 
GCCAATGACA TCATCAAATC ATCCAGCAAA GACGCCGCCA GGTTTTTGGA CTCTGTTCCA 
GCCACAGCTG TTGAATATTT CAGGGAATCG GCAGAGAAGC TGATCAAAGA GAAGGGAGCG 
GTGGATGCAC TCGCTGCTGC TCTGGCTCAC ATTTCCGGAG CCACGAGTCT GGAGCAGAGA 
TCTTTGCTCA ACTCTGACGC TGGCTACACC ACGATGCAGT TGCAGTGCTC CCAGGAGATG 
CACAATCTGG GTTATGCCTG GAGAACCATT AAAGAGCAGC TGGGAGAGGA CTTTGAAAAC 
CACATTCACA GAATGACCTT CCTCAAAGGC AAAACGGGAG TTTGTTTCGA TGTCCCGGCT 
GATAAAGTCA AAGCGATTCA GGATAATTGG AAAGATGGTC GCCGCTGGCA GCTGACTGTA 
GCCACAGAGC TCCCAGAGCT GGAGGAGAAG CAGTTCCCCA ACCGCGGAGA CAGAGGCGGG 
TTTAGAGGCG GGAGGGGGCG CGGCTTCGGT GGAAACGGAG GACGTAACGG CGGCTTCCGG 
AGCGGCGGTG GCGGCGGCTA CAACAACCGA GGAGGACACA AACGGACCTT CAACCAAGCG 
TTCGATTACT GA