TOP1 cDNA ORF clone, Phalacrocorax carbo(great cormorant)

Gene
Clones
gRNAs

Summary

Gene Symbol	TOP1
Entrez Gene ID	104046925
Full Name	DNA topoisomerase I
Gene Type	protein-coding
Organism	Phalacrocorax carbo(great cormorant)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009507166.1	XP_009505461.1	DNA topoisomerase 1

Pathways from BioSystems
Gene Ontology

The following TOP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPh211522	XM_009507166.1 Latest version!	Phalacrocorax carbo topoisomerase (DNA) I (TOP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TOP1 ( XM_009507166.1 ) cDNA ORF clone, Phalacrocorax carbo(great cormorant) -> XP_009505461.1 Phalacrocorax carbo topoisomerase (DNA) I (TOP1), mRNA.

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CloneID	OPh211522
Clone ID Related Accession (Same CDS sequence)	XM_009507166.1
Accession Version	XM_009507166.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-07
Organism	Phalacrocorax carbo(great cormorant)
Product	DNA topoisomerase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009124586.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Phalacrocorax carbo Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCGT CTTCTGGTGA TATAAAAATA AAGAAGGAAA AGGAAAATGG TTTCTCCAGT 
CCACCACGTA TTAAAGATGA ACCAGATGAT GATGGGTTTT ATGCATCTCC TAAAGAGGAC 
TCCAAACCCC TGAAGAGACC TCGAGAAGAT GATGATGCTG AGTACAAACC CAATAAAATC 
AAAACAGAAG ACATCAAGAA AGCAAAAAAA AGGAAACAAG AGGAGGAAGA GGACATCAAA 
GCAAAGAAAG CTAAGAGCAA AGATAAGAAA GTCCCTGAAG CGGACAAGAG AAAGAAGCCG 
AAAAAAGAAG AGGAGCAAAA ATGGAAATGG TGGGAAGAAG AGCGCTACCC TGAAGGCATT 
AAATGGAAGT TCCTAGAGCA CAAAGGTCCT GTATTTGCTC CACCATATGA ACCTCTGCCT 
GAAAATGTCA AGTTTTATTA TGATGGTAAG GTCATGAAGC TGAGCACCAA AGCAGAAGAA 
GTCGCCACAT TCTTTGCAAA AATGCTGGAT CATGAGTACA CTACAAAGGA GATCTTCAGG 
AAAAACTTTT TCAAAGACTG GAGAAAGGAA ATGACCTCTG AAGAGAAGAG CACTATCACC 
AACCTCAGCA AGTGTGACTT CACCCACATG AGCCAGTATT TCAAAGCTCA GACGGAAGCT 
AGGAAACAGA TGTCCAAGGA GGAGAAACAG AAAATCAAAG AGGAGAATGA GCGGCTATTG 
AAGGAATATG GTTACTGCGT GATGGATAAC CACAAGGAGA GGATTGCCAA CTTTAAGATT 
GAACCCCCAG GTCTCTTCCG AGGTCGTGGG AATCATCCCA AAATGGGCAT GTTGAAGAGA 
CGCATCATGC CAGAAGACAT CATCATTAAC TGCAGCAAGG ATTCCAAGAT TCCTTCCCCT 
CCCCCAGGAC ACAAATGGAA GGAGGTCCGG CATGATAACA AGGTTACCTG GCTGGTGTCA 
TGGACTGAGA ACATCCAAGG GTCTATCAAA TACATCATGT TGAACCCTAG CTCAAGAATC 
AAGGGTGAGA AGGACTGGCA GAAGTATGAG ACGGCTCGGA GGTTGAAGAA GTGCGTAGAT 
AAAATCAGAA ATCAATATCG AGAAGACTGG AAATCCAAAG AGATGAAAGT ACGGCAGAGG 
GCTGTGGCAC TGTACTTCAT TGATAAACTT GCCCTGAGAG CTGGTAACGA AAAAGAAGAA 
GGAGAGACAG CTGACACCGT GGGCTGCTGC TCTCTGAGAG TAGAACATAT CAAGCTGCAT 
CCTGAACTGG ATGGCCAGGA ATACGTGGTT GAGTTTGACT TCCTCGGAAA GGACTCCATC 
CGATACTACA ACAAAGTCCC TGTTGAGAAA AGGGTGTTCA AGAATCTTCA GCTGTTCATG 
GAGAACAAGC AGCCTGAAGA TGACCTCTTT GACCGCCTCA ATACCAGTAT CTTAAATAAA 
CACCTTCAAG ACCTTATGGA GGGACTGACA GCCAAGGTAT TCCGTACTTA CAACGCCTCC 
ATCACGCTAC AGCAGCAGCT CAAGGAGCTC ACTAATCCGG ATGATAACAT CCCAGCGAAG 
ATCCTTTCCT ATAACCGTGC CAACAGAGCG GTTGCCATTT TGTGTAACCA CCAGAGGGCT 
CCGCCGAAGA CCTTTGAGAA GTCCATGATG AACCTGCAAA GCAAGATCGA TGCCAAGAAG 
GAGCAGTTAG CTGATGCCAG GAGAGAACTG AAAAGCGCCA AAGCTGATGC CAAGGTCCGG 
AGGGATGAGA AGTCTAAAAA GACAGTGGAG AGCAAGAAGA AAGCAGTGCA GAGGATTGAG 
GAGCAACTGA TGAAGCTGGA GGTTCAGGCT ACAGATAGGG AGGAGAACAA GCAGATTGCT 
TTGGGCACCT CCAAACTCAA CTATCTGGAT CCCAGGATCT CCGTTGCTTG GTGTAAGAAG 
TGGGGAATTC CTATAGAGAA GATTTATAAC AAGACCCAAC GAGAAAAATT TGCCTGGGCT 
ATCGACATGG CAGAGGAAGA CTATGAGTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009505461.1
CDS	225..2237
Translation	MKSSSGDIKIKKEKENGFSSPPRIKDEPDDDGFYASPKEDSKPLKRPREDDDAEYKPNKIKTEDIKKAKKRKQEEEEDIKAKKAKSKDKKVPEADKRKKPKKEEEQKWKWWEEERYPEGIKWKFLEHKGPVFAPPYEPLPENVKFYYDGKVMKLSTKAEEVATFFAKMLDHEYTTKEIFRKNFFKDWRKEMTSEEKSTITNLSKCDFTHMSQYFKAQTEARKQMSKEEKQKIKEENERLLKEYGYCVMDNHKERIANFKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDSKIPSPPPGHKWKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKKCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHIKLHPELDGQEYVVEFDFLGKDSIRYYNKVPVEKRVFKNLQLFMENKQPEDDLFDRLNTSILNKHLQDLMEGLTAKVFRTYNASITLQQQLKELTNPDDNIPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQSKIDAKKEQLADARRELKSAKADAKVRRDEKSKKTVESKKKAVQRIEEQLMKLEVQATDREENKQIALGTSKLNYLDPRISVAWCKKWGIPIEKIYNKTQREKFAWAIDMAEEDYEF

Target ORF information:

RefSeq Version	XM_009507166.1
Organism	Phalacrocorax carbo(great cormorant)
Definition	Phalacrocorax carbo topoisomerase (DNA) I (TOP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009507166.1

ORF Insert Sequence:

ATGAAATCGT CTTCTGGTGA TATAAAAATA AAGAAGGAAA AGGAAAATGG TTTCTCCAGT 
CCACCACGTA TTAAAGATGA ACCAGATGAT GATGGGTTTT ATGCATCTCC TAAAGAGGAC 
TCCAAACCCC TGAAGAGACC TCGAGAAGAT GATGATGCTG AGTACAAACC CAATAAAATC 
AAAACAGAAG ACATCAAGAA AGCAAAAAAA AGGAAACAAG AGGAGGAAGA GGACATCAAA 
GCAAAGAAAG CTAAGAGCAA AGATAAGAAA GTCCCTGAAG CGGACAAGAG AAAGAAGCCG 
AAAAAAGAAG AGGAGCAAAA ATGGAAATGG TGGGAAGAAG AGCGCTACCC TGAAGGCATT 
AAATGGAAGT TCCTAGAGCA CAAAGGTCCT GTATTTGCTC CACCATATGA ACCTCTGCCT 
GAAAATGTCA AGTTTTATTA TGATGGTAAG GTCATGAAGC TGAGCACCAA AGCAGAAGAA 
GTCGCCACAT TCTTTGCAAA AATGCTGGAT CATGAGTACA CTACAAAGGA GATCTTCAGG 
AAAAACTTTT TCAAAGACTG GAGAAAGGAA ATGACCTCTG AAGAGAAGAG CACTATCACC 
AACCTCAGCA AGTGTGACTT CACCCACATG AGCCAGTATT TCAAAGCTCA GACGGAAGCT 
AGGAAACAGA TGTCCAAGGA GGAGAAACAG AAAATCAAAG AGGAGAATGA GCGGCTATTG 
AAGGAATATG GTTACTGCGT GATGGATAAC CACAAGGAGA GGATTGCCAA CTTTAAGATT 
GAACCCCCAG GTCTCTTCCG AGGTCGTGGG AATCATCCCA AAATGGGCAT GTTGAAGAGA 
CGCATCATGC CAGAAGACAT CATCATTAAC TGCAGCAAGG ATTCCAAGAT TCCTTCCCCT 
CCCCCAGGAC ACAAATGGAA GGAGGTCCGG CATGATAACA AGGTTACCTG GCTGGTGTCA 
TGGACTGAGA ACATCCAAGG GTCTATCAAA TACATCATGT TGAACCCTAG CTCAAGAATC 
AAGGGTGAGA AGGACTGGCA GAAGTATGAG ACGGCTCGGA GGTTGAAGAA GTGCGTAGAT 
AAAATCAGAA ATCAATATCG AGAAGACTGG AAATCCAAAG AGATGAAAGT ACGGCAGAGG 
GCTGTGGCAC TGTACTTCAT TGATAAACTT GCCCTGAGAG CTGGTAACGA AAAAGAAGAA 
GGAGAGACAG CTGACACCGT GGGCTGCTGC TCTCTGAGAG TAGAACATAT CAAGCTGCAT 
CCTGAACTGG ATGGCCAGGA ATACGTGGTT GAGTTTGACT TCCTCGGAAA GGACTCCATC 
CGATACTACA ACAAAGTCCC TGTTGAGAAA AGGGTGTTCA AGAATCTTCA GCTGTTCATG 
GAGAACAAGC AGCCTGAAGA TGACCTCTTT GACCGCCTCA ATACCAGTAT CTTAAATAAA 
CACCTTCAAG ACCTTATGGA GGGACTGACA GCCAAGGTAT TCCGTACTTA CAACGCCTCC 
ATCACGCTAC AGCAGCAGCT CAAGGAGCTC ACTAATCCGG ATGATAACAT CCCAGCGAAG 
ATCCTTTCCT ATAACCGTGC CAACAGAGCG GTTGCCATTT TGTGTAACCA CCAGAGGGCT 
CCGCCGAAGA CCTTTGAGAA GTCCATGATG AACCTGCAAA GCAAGATCGA TGCCAAGAAG 
GAGCAGTTAG CTGATGCCAG GAGAGAACTG AAAAGCGCCA AAGCTGATGC CAAGGTCCGG 
AGGGATGAGA AGTCTAAAAA GACAGTGGAG AGCAAGAAGA AAGCAGTGCA GAGGATTGAG 
GAGCAACTGA TGAAGCTGGA GGTTCAGGCT ACAGATAGGG AGGAGAACAA GCAGATTGCT 
TTGGGCACCT CCAAACTCAA CTATCTGGAT CCCAGGATCT CCGTTGCTTG GTGTAAGAAG 
TGGGGAATTC CTATAGAGAA GATTTATAAC AAGACCCAAC GAGAAAAATT TGCCTGGGCT 
ATCGACATGG CAGAGGAAGA CTATGAGTTT TAA