NIT2 cDNA ORF clone, Phalacrocorax carbo(great cormorant)

Gene
Clones
gRNAs

Summary

Gene Symbol	NIT2
Entrez Gene ID	104040016
Full Name	nitrilase family member 2
Gene Type	protein-coding
Organism	Phalacrocorax carbo(great cormorant)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009513115.1	XP_009511410.1	omega-amidase NIT2

Pathways from BioSystems
Gene Ontology

The following NIT2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NIT2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPh218184	XM_009513115.1 Latest version!	Phalacrocorax carbo nitrilase family, member 2 (NIT2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NIT2 ( XM_009513115.1 ) cDNA ORF clone, Phalacrocorax carbo(great cormorant) -> XP_009511410.1 Phalacrocorax carbo nitrilase family, member 2 (NIT2), mRNA.

Price & Availability↑

CloneID	OPh218184
Clone ID Related Accession (Same CDS sequence)	XM_009513115.1
Accession Version	XM_009513115.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-07
Organism	Phalacrocorax carbo(great cormorant)
Product	omega-amidase NIT2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009176742.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Phalacrocorax carbo Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTTTTC CTACAGCCCA AGACTTCCGC CTGGCTCTTA TTCAGCTTCA TGTATCTGCT 
GTTAAATCAG ATAACCTTCA ACGAGCCTGC GGACTGGTGA GAGAAGCATC AGCTAAAGGA 
GCAAAAGTTG TGGCTCTACC TGAATGCTTT AATTCTCCAT ACGGAACTAA ATACTTCAAG 
GAGTATGCGG AGAAGATCCC TGGAGAATCA ACACAGAAGC TGTCAGAAGT TGCAAAGGAG 
TGCAGCGTAT ATCTTGTTGG AGGATCCATT CCAGAAGAGG ATGGTGGGAA GCTGTATAAT 
ACATGTACTG TCTTCGGGCC TGATGGTGCT ATGTTGGCAA AGCATAGGAA GGTTCATTTG 
TTTGACATTA ATATTCCTGG GAAGATACAG TTCAAAGAGT CTGAAACACT GAGTCCAGGG 
AATAGTTTCT CCGTGTTTGA TACTCCATAC TGTAAAGTGG GCCTGGGCAT CTGCTATGAT 
ATCAGATTTG CTGAGATGGC TCAAGTCTAC GGACAGAAAG GTTGCCAGCT GCTGATATAT 
CCAGGGGCTT TTAACCTGAC AACAGGACCA GCTCATTGGG AACTGCTGCA AAGGGGGCGG 
GCTGTTGATA ATCAAGTCTA TGTGGCTACT GTATCTCCTG CTAGAGATGA AAAAGCATCA 
TACGTTGCCT GGGGACACAG CACTGTAGTA AATCCATGGG GTGAAGTCAT AGCCAAAGCT 
GGGGCTGAGG AAACAGTTAT ATACACAGAT ATAGATTTGA TGAAACTTGC AGAAATACGT 
CAACAAATTC CTATTTTAAG CCAGAAGCGT TGTGATCTCT ATGGCATAGA GATGAAAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009511410.1
CDS	1..843
Translation	MCFPTAQDFRLALIQLHVSAVKSDNLQRACGLVREASAKGAKVVALPECFNSPYGTKYFKEYAEKIPGESTQKLSEVAKECSVYLVGGSIPEEDGGKLYNTCTVFGPDGAMLAKHRKVHLFDINIPGKIQFKESETLSPGNSFSVFDTPYCKVGLGICYDIRFAEMAQVYGQKGCQLLIYPGAFNLTTGPAHWELLQRGRAVDNQVYVATVSPARDEKASYVAWGHSTVVNPWGEVIAKAGAEETVIYTDIDLMKLAEIRQQIPILSQKRCDLYGIEMKK

Target ORF information:

RefSeq Version	XM_009513115.1
Organism	Phalacrocorax carbo(great cormorant)
Definition	Phalacrocorax carbo nitrilase family, member 2 (NIT2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009513115.1

ORF Insert Sequence:

ATGTGTTTTC CTACAGCCCA AGACTTCCGC CTGGCTCTTA TTCAGCTTCA TGTATCTGCT 
GTTAAATCAG ATAACCTTCA ACGAGCCTGC GGACTGGTGA GAGAAGCATC AGCTAAAGGA 
GCAAAAGTTG TGGCTCTACC TGAATGCTTT AATTCTCCAT ACGGAACTAA ATACTTCAAG 
GAGTATGCGG AGAAGATCCC TGGAGAATCA ACACAGAAGC TGTCAGAAGT TGCAAAGGAG 
TGCAGCGTAT ATCTTGTTGG AGGATCCATT CCAGAAGAGG ATGGTGGGAA GCTGTATAAT 
ACATGTACTG TCTTCGGGCC TGATGGTGCT ATGTTGGCAA AGCATAGGAA GGTTCATTTG 
TTTGACATTA ATATTCCTGG GAAGATACAG TTCAAAGAGT CTGAAACACT GAGTCCAGGG 
AATAGTTTCT CCGTGTTTGA TACTCCATAC TGTAAAGTGG GCCTGGGCAT CTGCTATGAT 
ATCAGATTTG CTGAGATGGC TCAAGTCTAC GGACAGAAAG GTTGCCAGCT GCTGATATAT 
CCAGGGGCTT TTAACCTGAC AACAGGACCA GCTCATTGGG AACTGCTGCA AAGGGGGCGG 
GCTGTTGATA ATCAAGTCTA TGTGGCTACT GTATCTCCTG CTAGAGATGA AAAAGCATCA 
TACGTTGCCT GGGGACACAG CACTGTAGTA AATCCATGGG GTGAAGTCAT AGCCAAAGCT 
GGGGCTGAGG AAACAGTTAT ATACACAGAT ATAGATTTGA TGAAACTTGC AGAAATACGT 
CAACAAATTC CTATTTTAAG CCAGAAGCGT TGTGATCTCT ATGGCATAGA GATGAAAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.