MAN2C1 cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAN2C1
Entrez Gene ID	102457634
Full Name	mannosidase alpha class 2C member 1
Gene Type	protein-coding
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025186236.1	XP_025042021.1	alpha-mannosidase 2C1
XM_014575126.1	XP_014430612.1	alpha-mannosidase 2C1

Pathways from BioSystems
Gene Ontology

The following MAN2C1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAN2C1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPe32262	XM_025186236.1 Latest version!	Pelodiscus sinensis mannosidase alpha class 2C member 1 (MAN2C1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPe32262	XM_014575126.1 Latest version!	Pelodiscus sinensis mannosidase, alpha, class 2C, member 1 (MAN2C1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAN2C1 ( XM_025186236.1 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_025042021.1 Pelodiscus sinensis mannosidase alpha class 2C member 1 (MAN2C1), mRNA.

Price & Availability↑

CloneID	OPe32262
Clone ID Related Accession (Same CDS sequence)	XM_025186236.1 , XM_014575126.1
Accession Version	XM_025186236.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	alpha-mannosidase 2C1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005855909.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pelodiscus sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGCCCAGGA AACTCAATTA CCTCAAGGTG TTTTGCTTTA TTGCCTATCA TCTTTACTGC 
AGGCTTTTTG GGGACACCTA CCCTTTGGTG TCCCTCTCCT GCTTCCAGAG CCCACAGCGA 
ATCCCATATG ATGAAGCTGT CAGACAAGAA TTCAGACCTG TGAAAGTTGG GGACTCCTTT 
GGACCTACGT GGTGGACCTG CTGGTTTAAA GTGGAGCTTA GCATCCCCAG GGAATGGACG 
GGAAAGGAAG TGCACCTCCT GTGGGAGAGC GACGGGGAGG GGATGGTGTG GCGGGACACA 
CAGCCAGTCC AGGGTTTGAC GAAAGAAGGG GAGAAGACCA GCTATATCCT GACAGAAGCC 
CTCAAAGAGA CAGACCCTCA CAGTCTTACT GTCTACGTGG AAGTTGCCTG CAATGGTCTC 
TTCGGGGCAG GAAAAGGCAG CCTCGTTGCA CCTCCTGACC CTGAGAAGAA GTTCACCCTG 
AACCAGGCGG AACTTGTGGT CTTCCACAGG GATGTCTATG AGCTGCTGGT GGATTTTGAG 
ATACTAGTGG ACATGGCCAA GTTCCTTGGG GAGGAAAACC AGCGGAGCTT CCAGGCGCTG 
TACGTGGCCA ATCAGATGGT TAATCTGCTT GACGTCACGG ACCCCGCTAC CTTCCCTGCA 
GCGCGGCAGC TCGCTTCCTC CATCTTCAGC CAGAAGAACG GAGACAGCCA GCACACGATC 
CACGCGATGG GGCACTGCCA CATCGATTCT GCCTGGCTGT GGCCATACGA GGAGTCTGTC 
CGCAAGTGCG CTCGCAGCTG GGTGACTGTC ATACGCCTGA TGGAGAAGAA CCCGGAGTTC 
ACGTTTGTCT GCTCACAGGC CCAGCAGTTT GAGTGGGTGA GGAACCGGTA TCCTGGCCTG 
TATGCTCAGA TTCAGGACTT TGCCAAAGAA GGGCGGTTCA TACCTGTCGG CGGCACCTGG 
GTAGAAATGG ACGGGAATCT TCCCAGTGGC GAATCCATGG TGCGTCAGTT CCTGCTGGGA 
CAGCGGTTTT TCCAGCAGCA GTTTGGGAGG CTCTGCTCAG AGTTCTGGCT GCCCGACACG 
TTTGGCTACT CGGCCCAGCT GCCCCAGCTG ATGCGTGGCT GTGGGATACG ACGGTTCCTC 
ACTCAGAAAC TCAGCTGGAG CCTGGTGAAC ACCTTCCCGC ACAGCACCTT TTTCTGGGAA 
GGCATCGACG GCTCCCAAGT TCTGACCCAT TTCCCGCCAG GCAATTCCTA TGGGCTACAA 
GGTCAAGTGG AGGAGATGCT GAAAACCATG AACAACAACA GAGACAAGGG CCAGGTGAAC 
CACAGCACCG CGCTCTTTGG GTTTGGAGAC GGAGGCGGCG GCCCCACCCA GAAGATGCTG 
GACAGGCTGA CAAGAATGAG AGACGTAGAT GGGTTACCCA GGGTTCAGAT GTCCACACCG 
GATCAGTTTT TCTCTGTGCT GGAGAAGGAA GAGCCCCAGC TGTGCACCTG GATAGGAGAA 
TTGTTCCTGG AGCTGCACAA CGGCACTTAT ACGACCCAGG CCCAGATAAA GAAAGAGAAT 
CGGGAGTGCG AGCGGATACT CCATGATGTT GAAGTACTGA GTAGTTTTGC TCTGACCCAG 
AACAGTACCT TCCCATATCC TTCCAGCAAA CTGGAGCATC TCTGGAGGCT GCTGCTCCTG 
AATCAGTTTC ATGACGTGCT GCCGGGCAGC TGCATCCAGC TAGTTGTGGA GGATGCCCTG 
AGGTATTACG CAGAAATCCT TTTGCCGACT GGTCCAGAGA CAGCAGAAAG CCTCCTTGTT 
CTAAACACTT TGCCCTGGGA ACGGACTGAA GTCATCTCCA GGACCGGAGA AGAGACCTTA 
GCCCTGGTGA AGGTGCCTAG CATGGGCTAC TCTGCCGTGA AGGATTCCTT AACACCGCCT 
CATCCCGTGA CAGTGGTGAA GCAGGCGGAT GGCTCTGTTA CCATGGAGAA CGGGGTGATC 
GCAGCTCACC TGGACTCCAT GGGACGCGTG ATATCGCTTC GCTTGGTGCA CTCGGGGAGA 
GAGTCGGTCC AGGATGGCCA CTGTGCTAAC CAGTTTGTGA TATTTGATGA CATTCCCTTG 
TACTGGGATG CTTGGGATGC GATGGATTAT CACCTGGAAA CCAGGAAGCC AATCACATCT 
CTTCTGAAGC CCCTGGAAAT CGTCCTAGCT GGGGGCCTGC GTGGAAGCGT GAAATTCTCT 
CTGCAGATCA GTGAGCGGAG TGTCATCACC CAGGAGGTGA TCCTGGATGC CATGTGTCCT 
TATCTTCGGT TCCTGACCCA GGTGGACTGG AATGAGGCTC ACCGATTCCT GAAGGTGGAG 
TTCCCCTTGC GGGTTCGGAG CATGAATGCA ACGTATGAGA TTCAGTTTGG GCACCTGCAG 
AGGCCGACGC ACTGGAACAC GTCCTGGGAC TGGGCTCGAT TCGAGGTGTG GAGTCACAAG 
TGGATGGACC TCGGGATCAG CTGCCTGCCC ATTCTGCCAG CGCCGCGGGC GTTCGCTCTC 
ATGGCAGGAC TCCCCGCAGC ACTGCCACGG AGCCTGCGGG GCAGGGCGCA GGAGACCCTG 
CTGCGAGACG GGGTGGCAGG GCGGCTTGAC GCTAGCCAGG GCTGTGCACG TGGACTCGCG 
CTTTCAGGGA GCGGGGCTGG GTTTAGTCAC GGCCAGGGGA AGAGGCTCCA CTCCATTAAT 
CCGCCCCTCT CCTCCAGGCT GAGAGCCCCA AAGTCACCTG ATGCCACTGC AGATATTGGG 
CACCATCAGT TCACCTACGC CGTGATGCCC CACAGGGGCT CCTTCCAGGA GGCGGGTGTA 
ATCCATTGTG CCTACAGTTT GAACTACCCT CTGCACACTA TTCCTGTTGT CTCGGTGCCA 
TGTCCAGCCT GGAGCGCCTT CTCCGTGGAC TCCCCAGCTG TGGTGCTAGA AACCGTCAAA 
CAGGCTGAGG ACAGGCTGGA CAGCCTGGTG TTGAGGCTGT ATGAGTCATA TGGCAGCACA 
GTGGTCACAT GGCTCCGGAC TGCCCTTCCC ATCAAGGAAG CAATCGACTG TGACCTCCTG 
GAACGGCCGG ACCCCAGCAG CCATCTGCCA CTGGGGGAGC ATGGCCTAAC ACTCTCCTTT 
ACGCCGTTCC AAGTGCGCTC TCTCCTCCTG ATCTTGAGGC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025042021.1
CDS	1..3165
Translation	MPRKLNYLKVFCFIAYHLYCRLFGDTYPLVSLSCFQSPQRIPYDEAVRQEFRPVKVGDSFGPTWWTCWFKVELSIPREWTGKEVHLLWESDGEGMVWRDTQPVQGLTKEGEKTSYILTEALKETDPHSLTVYVEVACNGLFGAGKGSLVAPPDPEKKFTLNQAELVVFHRDVYELLVDFEILVDMAKFLGEENQRSFQALYVANQMVNLLDVTDPATFPAARQLASSIFSQKNGDSQHTIHAMGHCHIDSAWLWPYEESVRKCARSWVTVIRLMEKNPEFTFVCSQAQQFEWVRNRYPGLYAQIQDFAKEGRFIPVGGTWVEMDGNLPSGESMVRQFLLGQRFFQQQFGRLCSEFWLPDTFGYSAQLPQLMRGCGIRRFLTQKLSWSLVNTFPHSTFFWEGIDGSQVLTHFPPGNSYGLQGQVEEMLKTMNNNRDKGQVNHSTALFGFGDGGGGPTQKMLDRLTRMRDVDGLPRVQMSTPDQFFSVLEKEEPQLCTWIGELFLELHNGTYTTQAQIKKENRECERILHDVEVLSSFALTQNSTFPYPSSKLEHLWRLLLLNQFHDVLPGSCIQLVVEDALRYYAEILLPTGPETAESLLVLNTLPWERTEVISRTGEETLALVKVPSMGYSAVKDSLTPPHPVTVVKQADGSVTMENGVIAAHLDSMGRVISLRLVHSGRESVQDGHCANQFVIFDDIPLYWDAWDAMDYHLETRKPITSLLKPLEIVLAGGLRGSVKFSLQISERSVITQEVILDAMCPYLRFLTQVDWNEAHRFLKVEFPLRVRSMNATYEIQFGHLQRPTHWNTSWDWARFEVWSHKWMDLGISCLPILPAPRAFALMAGLPAALPRSLRGRAQETLLRDGVAGRLDASQGCARGLALSGSGAGFSHGQGKRLHSINPPLSSRLRAPKSPDATADIGHHQFTYAVMPHRGSFQEAGVIHCAYSLNYPLHTIPVVSVPCPAWSAFSVDSPAVVLETVKQAEDRLDSLVLRLYESYGSTVVTWLRTALPIKEAIDCDLLERPDPSSHLPLGEHGLTLSFTPFQVRSLLLILRQ

Target ORF information:

RefSeq Version	XM_025186236.1
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis mannosidase alpha class 2C member 1 (MAN2C1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025186236.1

ORF Insert Sequence:

ATGCCCAGGA AACTCAATTA CCTCAAGGTG TTTTGCTTTA TTGCCTATCA TCTTTACTGC 
AGGCTTTTTG GGGACACCTA CCCTTTGGTG TCCCTCTCCT GCTTCCAGAG CCCACAGCGA 
ATCCCATATG ATGAAGCTGT CAGACAAGAA TTCAGACCTG TGAAAGTTGG GGACTCCTTT 
GGACCTACGT GGTGGACCTG CTGGTTTAAA GTGGAGCTTA GCATCCCCAG GGAATGGACG 
GGAAAGGAAG TGCACCTCCT GTGGGAGAGC GACGGGGAGG GGATGGTGTG GCGGGACACA 
CAGCCAGTCC AGGGTTTGAC GAAAGAAGGG GAGAAGACCA GCTATATCCT GACAGAAGCC 
CTCAAAGAGA CAGACCCTCA CAGTCTTACT GTCTACGTGG AAGTTGCCTG CAATGGTCTC 
TTCGGGGCAG GAAAAGGCAG CCTCGTTGCA CCTCCTGACC CTGAGAAGAA GTTCACCCTG 
AACCAGGCGG AACTTGTGGT CTTCCACAGG GATGTCTATG AGCTGCTGGT GGATTTTGAG 
ATACTAGTGG ACATGGCCAA GTTCCTTGGG GAGGAAAACC AGCGGAGCTT CCAGGCGCTG 
TACGTGGCCA ATCAGATGGT TAATCTGCTT GACGTCACGG ACCCCGCTAC CTTCCCTGCA 
GCGCGGCAGC TCGCTTCCTC CATCTTCAGC CAGAAGAACG GAGACAGCCA GCACACGATC 
CACGCGATGG GGCACTGCCA CATCGATTCT GCCTGGCTGT GGCCATACGA GGAGTCTGTC 
CGCAAGTGCG CTCGCAGCTG GGTGACTGTC ATACGCCTGA TGGAGAAGAA CCCGGAGTTC 
ACGTTTGTCT GCTCACAGGC CCAGCAGTTT GAGTGGGTGA GGAACCGGTA TCCTGGCCTG 
TATGCTCAGA TTCAGGACTT TGCCAAAGAA GGGCGGTTCA TACCTGTCGG CGGCACCTGG 
GTAGAAATGG ACGGGAATCT TCCCAGTGGC GAATCCATGG TGCGTCAGTT CCTGCTGGGA 
CAGCGGTTTT TCCAGCAGCA GTTTGGGAGG CTCTGCTCAG AGTTCTGGCT GCCCGACACG 
TTTGGCTACT CGGCCCAGCT GCCCCAGCTG ATGCGTGGCT GTGGGATACG ACGGTTCCTC 
ACTCAGAAAC TCAGCTGGAG CCTGGTGAAC ACCTTCCCGC ACAGCACCTT TTTCTGGGAA 
GGCATCGACG GCTCCCAAGT TCTGACCCAT TTCCCGCCAG GCAATTCCTA TGGGCTACAA 
GGTCAAGTGG AGGAGATGCT GAAAACCATG AACAACAACA GAGACAAGGG CCAGGTGAAC 
CACAGCACCG CGCTCTTTGG GTTTGGAGAC GGAGGCGGCG GCCCCACCCA GAAGATGCTG 
GACAGGCTGA CAAGAATGAG AGACGTAGAT GGGTTACCCA GGGTTCAGAT GTCCACACCG 
GATCAGTTTT TCTCTGTGCT GGAGAAGGAA GAGCCCCAGC TGTGCACCTG GATAGGAGAA 
TTGTTCCTGG AGCTGCACAA CGGCACTTAT ACGACCCAGG CCCAGATAAA GAAAGAGAAT 
CGGGAGTGCG AGCGGATACT CCATGATGTT GAAGTACTGA GTAGTTTTGC TCTGACCCAG 
AACAGTACCT TCCCATATCC TTCCAGCAAA CTGGAGCATC TCTGGAGGCT GCTGCTCCTG 
AATCAGTTTC ATGACGTGCT GCCGGGCAGC TGCATCCAGC TAGTTGTGGA GGATGCCCTG 
AGGTATTACG CAGAAATCCT TTTGCCGACT GGTCCAGAGA CAGCAGAAAG CCTCCTTGTT 
CTAAACACTT TGCCCTGGGA ACGGACTGAA GTCATCTCCA GGACCGGAGA AGAGACCTTA 
GCCCTGGTGA AGGTGCCTAG CATGGGCTAC TCTGCCGTGA AGGATTCCTT AACACCGCCT 
CATCCCGTGA CAGTGGTGAA GCAGGCGGAT GGCTCTGTTA CCATGGAGAA CGGGGTGATC 
GCAGCTCACC TGGACTCCAT GGGACGCGTG ATATCGCTTC GCTTGGTGCA CTCGGGGAGA 
GAGTCGGTCC AGGATGGCCA CTGTGCTAAC CAGTTTGTGA TATTTGATGA CATTCCCTTG 
TACTGGGATG CTTGGGATGC GATGGATTAT CACCTGGAAA CCAGGAAGCC AATCACATCT 
CTTCTGAAGC CCCTGGAAAT CGTCCTAGCT GGGGGCCTGC GTGGAAGCGT GAAATTCTCT 
CTGCAGATCA GTGAGCGGAG TGTCATCACC CAGGAGGTGA TCCTGGATGC CATGTGTCCT 
TATCTTCGGT TCCTGACCCA GGTGGACTGG AATGAGGCTC ACCGATTCCT GAAGGTGGAG 
TTCCCCTTGC GGGTTCGGAG CATGAATGCA ACGTATGAGA TTCAGTTTGG GCACCTGCAG 
AGGCCGACGC ACTGGAACAC GTCCTGGGAC TGGGCTCGAT TCGAGGTGTG GAGTCACAAG 
TGGATGGACC TCGGGATCAG CTGCCTGCCC ATTCTGCCAG CGCCGCGGGC GTTCGCTCTC 
ATGGCAGGAC TCCCCGCAGC ACTGCCACGG AGCCTGCGGG GCAGGGCGCA GGAGACCCTG 
CTGCGAGACG GGGTGGCAGG GCGGCTTGAC GCTAGCCAGG GCTGTGCACG TGGACTCGCG 
CTTTCAGGGA GCGGGGCTGG GTTTAGTCAC GGCCAGGGGA AGAGGCTCCA CTCCATTAAT 
CCGCCCCTCT CCTCCAGGCT GAGAGCCCCA AAGTCACCTG ATGCCACTGC AGATATTGGG 
CACCATCAGT TCACCTACGC CGTGATGCCC CACAGGGGCT CCTTCCAGGA GGCGGGTGTA 
ATCCATTGTG CCTACAGTTT GAACTACCCT CTGCACACTA TTCCTGTTGT CTCGGTGCCA 
TGTCCAGCCT GGAGCGCCTT CTCCGTGGAC TCCCCAGCTG TGGTGCTAGA AACCGTCAAA 
CAGGCTGAGG ACAGGCTGGA CAGCCTGGTG TTGAGGCTGT ATGAGTCATA TGGCAGCACA 
GTGGTCACAT GGCTCCGGAC TGCCCTTCCC ATCAAGGAAG CAATCGACTG TGACCTCCTG 
GAACGGCCGG ACCCCAGCAG CCATCTGCCA CTGGGGGAGC ATGGCCTAAC ACTCTCCTTT 
ACGCCGTTCC AAGTGCGCTC TCTCCTCCTG ATCTTGAGGC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2C1 ( XM_014575126.1 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_014430612.1 Pelodiscus sinensis mannosidase, alpha, class 2C, member 1 (MAN2C1), mRNA.

Price & Availability↑

CloneID	OPe32262
Clone ID Related Accession (Same CDS sequence)	XM_025186236.1 , XM_014575126.1
Accession Version	XM_014575126.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-02
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	alpha-mannosidase 2C1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005855909.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pelodiscus sinensis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGCCCAGGA AACTCAATTA CCTCAAGGTG TTTTGCTTTA TTGCCTATCA TCTTTACTGC 
AGGCTTTTTG GGGACACCTA CCCTTTGGTG TCCCTCTCCT GCTTCCAGAG CCCACAGCGA 
ATCCCATATG ATGAAGCTGT CAGACAAGAA TTCAGACCTG TGAAAGTTGG GGACTCCTTT 
GGACCTACGT GGTGGACCTG CTGGTTTAAA GTGGAGCTTA GCATCCCCAG GGAATGGACG 
GGAAAGGAAG TGCACCTCCT GTGGGAGAGC GACGGGGAGG GGATGGTGTG GCGGGACACA 
CAGCCAGTCC AGGGTTTGAC GAAAGAAGGG GAGAAGACCA GCTATATCCT GACAGAAGCC 
CTCAAAGAGA CAGACCCTCA CAGTCTTACT GTCTACGTGG AAGTTGCCTG CAATGGTCTC 
TTCGGGGCAG GAAAAGGCAG CCTCGTTGCA CCTCCTGACC CTGAGAAGAA GTTCACCCTG 
AACCAGGCGG AACTTGTGGT CTTCCACAGG GATGTCTATG AGCTGCTGGT GGATTTTGAG 
ATACTAGTGG ACATGGCCAA GTTCCTTGGG GAGGAAAACC AGCGGAGCTT CCAGGCGCTG 
TACGTGGCCA ATCAGATGGT TAATCTGCTT GACGTCACGG ACCCCGCTAC CTTCCCTGCA 
GCGCGGCAGC TCGCTTCCTC CATCTTCAGC CAGAAGAACG GAGACAGCCA GCACACGATC 
CACGCGATGG GGCACTGCCA CATCGATTCT GCCTGGCTGT GGCCATACGA GGAGTCTGTC 
CGCAAGTGCG CTCGCAGCTG GGTGACTGTC ATACGCCTGA TGGAGAAGAA CCCGGAGTTC 
ACGTTTGTCT GCTCACAGGC CCAGCAGTTT GAGTGGGTGA GGAACCGGTA TCCTGGCCTG 
TATGCTCAGA TTCAGGACTT TGCCAAAGAA GGGCGGTTCA TACCTGTCGG CGGCACCTGG 
GTAGAAATGG ACGGGAATCT TCCCAGTGGC GAATCCATGG TGCGTCAGTT CCTGCTGGGA 
CAGCGGTTTT TCCAGCAGCA GTTTGGGAGG CTCTGCTCAG AGTTCTGGCT GCCCGACACG 
TTTGGCTACT CGGCCCAGCT GCCCCAGCTG ATGCGTGGCT GTGGGATACG ACGGTTCCTC 
ACTCAGAAAC TCAGCTGGAG CCTGGTGAAC ACCTTCCCGC ACAGCACCTT TTTCTGGGAA 
GGCATCGACG GCTCCCAAGT TCTGACCCAT TTCCCGCCAG GCAATTCCTA TGGGCTACAA 
GGTCAAGTGG AGGAGATGCT GAAAACCATG AACAACAACA GAGACAAGGG CCAGGTGAAC 
CACAGCACCG CGCTCTTTGG GTTTGGAGAC GGAGGCGGCG GCCCCACCCA GAAGATGCTG 
GACAGGCTGA CAAGAATGAG AGACGTAGAT GGGTTACCCA GGGTTCAGAT GTCCACACCG 
GATCAGTTTT TCTCTGTGCT GGAGAAGGAA GAGCCCCAGC TGTGCACCTG GATAGGAGAA 
TTGTTCCTGG AGCTGCACAA CGGCACTTAT ACGACCCAGG CCCAGATAAA GAAAGAGAAT 
CGGGAGTGCG AGCGGATACT CCATGATGTT GAAGTACTGA GTAGTTTTGC TCTGACCCAG 
AACAGTACCT TCCCATATCC TTCCAGCAAA CTGGAGCATC TCTGGAGGCT GCTGCTCCTG 
AATCAGTTTC ATGACGTGCT GCCGGGCAGC TGCATCCAGC TAGTTGTGGA GGATGCCCTG 
AGGTATTACG CAGAAATCCT TTTGCCGACT GGTCCAGAGA CAGCAGAAAG CCTCCTTGTT 
CTAAACACTT TGCCCTGGGA ACGGACTGAA GTCATCTCCA GGACCGGAGA AGAGACCTTA 
GCCCTGGTGA AGGTGCCTAG CATGGGCTAC TCTGCCGTGA AGGATTCCTT AACACCGCCT 
CATCCCGTGA CAGTGGTGAA GCAGGCGGAT GGCTCTGTTA CCATGGAGAA CGGGGTGATC 
GCAGCTCACC TGGACTCCAT GGGACGCGTG ATATCGCTTC GCTTGGTGCA CTCGGGGAGA 
GAGTCGGTCC AGGATGGCCA CTGTGCTAAC CAGTTTGTGA TATTTGATGA CATTCCCTTG 
TACTGGGATG CTTGGGATGC GATGGATTAT CACCTGGAAA CCAGGAAGCC AATCACATCT 
CTTCTGAAGC CCCTGGAAAT CGTCCTAGCT GGGGGCCTGC GTGGAAGCGT GAAATTCTCT 
CTGCAGATCA GTGAGCGGAG TGTCATCACC CAGGAGGTGA TCCTGGATGC CATGTGTCCT 
TATCTTCGGT TCCTGACCCA GGTGGACTGG AATGAGGCTC ACCGATTCCT GAAGGTGGAG 
TTCCCCTTGC GGGTTCGGAG CATGAATGCA ACGTATGAGA TTCAGTTTGG GCACCTGCAG 
AGGCCGACGC ACTGGAACAC GTCCTGGGAC TGGGCTCGAT TCGAGGTGTG GAGTCACAAG 
TGGATGGACC TCGGGATCAG CTGCCTGCCC ATTCTGCCAG CGCCGCGGGC GTTCGCTCTC 
ATGGCAGGAC TCCCCGCAGC ACTGCCACGG AGCCTGCGGG GCAGGGCGCA GGAGACCCTG 
CTGCGAGACG GGGTGGCAGG GCGGCTTGAC GCTAGCCAGG GCTGTGCACG TGGACTCGCG 
CTTTCAGGGA GCGGGGCTGG GTTTAGTCAC GGCCAGGGGA AGAGGCTCCA CTCCATTAAT 
CCGCCCCTCT CCTCCAGGCT GAGAGCCCCA AAGTCACCTG ATGCCACTGC AGATATTGGG 
CACCATCAGT TCACCTACGC CGTGATGCCC CACAGGGGCT CCTTCCAGGA GGCGGGTGTA 
ATCCATTGTG CCTACAGTTT GAACTACCCT CTGCACACTA TTCCTGTTGT CTCGGTGCCA 
TGTCCAGCCT GGAGCGCCTT CTCCGTGGAC TCCCCAGCTG TGGTGCTAGA AACCGTCAAA 
CAGGCTGAGG ACAGGCTGGA CAGCCTGGTG TTGAGGCTGT ATGAGTCATA TGGCAGCACA 
GTGGTCACAT GGCTCCGGAC TGCCCTTCCC ATCAAGGAAG CAATCGACTG TGACCTCCTG 
GAACGGCCGG ACCCCAGCAG CCATCTGCCA CTGGGGGAGC ATGGCCTAAC ACTCTCCTTT 
ACGCCGTTCC AAGTGCGCTC TCTCCTCCTG ATCTTGAGGC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014430612.1
CDS	1..3165
Translation	MPRKLNYLKVFCFIAYHLYCRLFGDTYPLVSLSCFQSPQRIPYDEAVRQEFRPVKVGDSFGPTWWTCWFKVELSIPREWTGKEVHLLWESDGEGMVWRDTQPVQGLTKEGEKTSYILTEALKETDPHSLTVYVEVACNGLFGAGKGSLVAPPDPEKKFTLNQAELVVFHRDVYELLVDFEILVDMAKFLGEENQRSFQALYVANQMVNLLDVTDPATFPAARQLASSIFSQKNGDSQHTIHAMGHCHIDSAWLWPYEESVRKCARSWVTVIRLMEKNPEFTFVCSQAQQFEWVRNRYPGLYAQIQDFAKEGRFIPVGGTWVEMDGNLPSGESMVRQFLLGQRFFQQQFGRLCSEFWLPDTFGYSAQLPQLMRGCGIRRFLTQKLSWSLVNTFPHSTFFWEGIDGSQVLTHFPPGNSYGLQGQVEEMLKTMNNNRDKGQVNHSTALFGFGDGGGGPTQKMLDRLTRMRDVDGLPRVQMSTPDQFFSVLEKEEPQLCTWIGELFLELHNGTYTTQAQIKKENRECERILHDVEVLSSFALTQNSTFPYPSSKLEHLWRLLLLNQFHDVLPGSCIQLVVEDALRYYAEILLPTGPETAESLLVLNTLPWERTEVISRTGEETLALVKVPSMGYSAVKDSLTPPHPVTVVKQADGSVTMENGVIAAHLDSMGRVISLRLVHSGRESVQDGHCANQFVIFDDIPLYWDAWDAMDYHLETRKPITSLLKPLEIVLAGGLRGSVKFSLQISERSVITQEVILDAMCPYLRFLTQVDWNEAHRFLKVEFPLRVRSMNATYEIQFGHLQRPTHWNTSWDWARFEVWSHKWMDLGISCLPILPAPRAFALMAGLPAALPRSLRGRAQETLLRDGVAGRLDASQGCARGLALSGSGAGFSHGQGKRLHSINPPLSSRLRAPKSPDATADIGHHQFTYAVMPHRGSFQEAGVIHCAYSLNYPLHTIPVVSVPCPAWSAFSVDSPAVVLETVKQAEDRLDSLVLRLYESYGSTVVTWLRTALPIKEAIDCDLLERPDPSSHLPLGEHGLTLSFTPFQVRSLLLILRQ

Target ORF information:

RefSeq Version	XM_014575126.1
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis mannosidase, alpha, class 2C, member 1 (MAN2C1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014575126.1

ORF Insert Sequence:

ATGCCCAGGA AACTCAATTA CCTCAAGGTG TTTTGCTTTA TTGCCTATCA TCTTTACTGC 
AGGCTTTTTG GGGACACCTA CCCTTTGGTG TCCCTCTCCT GCTTCCAGAG CCCACAGCGA 
ATCCCATATG ATGAAGCTGT CAGACAAGAA TTCAGACCTG TGAAAGTTGG GGACTCCTTT 
GGACCTACGT GGTGGACCTG CTGGTTTAAA GTGGAGCTTA GCATCCCCAG GGAATGGACG 
GGAAAGGAAG TGCACCTCCT GTGGGAGAGC GACGGGGAGG GGATGGTGTG GCGGGACACA 
CAGCCAGTCC AGGGTTTGAC GAAAGAAGGG GAGAAGACCA GCTATATCCT GACAGAAGCC 
CTCAAAGAGA CAGACCCTCA CAGTCTTACT GTCTACGTGG AAGTTGCCTG CAATGGTCTC 
TTCGGGGCAG GAAAAGGCAG CCTCGTTGCA CCTCCTGACC CTGAGAAGAA GTTCACCCTG 
AACCAGGCGG AACTTGTGGT CTTCCACAGG GATGTCTATG AGCTGCTGGT GGATTTTGAG 
ATACTAGTGG ACATGGCCAA GTTCCTTGGG GAGGAAAACC AGCGGAGCTT CCAGGCGCTG 
TACGTGGCCA ATCAGATGGT TAATCTGCTT GACGTCACGG ACCCCGCTAC CTTCCCTGCA 
GCGCGGCAGC TCGCTTCCTC CATCTTCAGC CAGAAGAACG GAGACAGCCA GCACACGATC 
CACGCGATGG GGCACTGCCA CATCGATTCT GCCTGGCTGT GGCCATACGA GGAGTCTGTC 
CGCAAGTGCG CTCGCAGCTG GGTGACTGTC ATACGCCTGA TGGAGAAGAA CCCGGAGTTC 
ACGTTTGTCT GCTCACAGGC CCAGCAGTTT GAGTGGGTGA GGAACCGGTA TCCTGGCCTG 
TATGCTCAGA TTCAGGACTT TGCCAAAGAA GGGCGGTTCA TACCTGTCGG CGGCACCTGG 
GTAGAAATGG ACGGGAATCT TCCCAGTGGC GAATCCATGG TGCGTCAGTT CCTGCTGGGA 
CAGCGGTTTT TCCAGCAGCA GTTTGGGAGG CTCTGCTCAG AGTTCTGGCT GCCCGACACG 
TTTGGCTACT CGGCCCAGCT GCCCCAGCTG ATGCGTGGCT GTGGGATACG ACGGTTCCTC 
ACTCAGAAAC TCAGCTGGAG CCTGGTGAAC ACCTTCCCGC ACAGCACCTT TTTCTGGGAA 
GGCATCGACG GCTCCCAAGT TCTGACCCAT TTCCCGCCAG GCAATTCCTA TGGGCTACAA 
GGTCAAGTGG AGGAGATGCT GAAAACCATG AACAACAACA GAGACAAGGG CCAGGTGAAC 
CACAGCACCG CGCTCTTTGG GTTTGGAGAC GGAGGCGGCG GCCCCACCCA GAAGATGCTG 
GACAGGCTGA CAAGAATGAG AGACGTAGAT GGGTTACCCA GGGTTCAGAT GTCCACACCG 
GATCAGTTTT TCTCTGTGCT GGAGAAGGAA GAGCCCCAGC TGTGCACCTG GATAGGAGAA 
TTGTTCCTGG AGCTGCACAA CGGCACTTAT ACGACCCAGG CCCAGATAAA GAAAGAGAAT 
CGGGAGTGCG AGCGGATACT CCATGATGTT GAAGTACTGA GTAGTTTTGC TCTGACCCAG 
AACAGTACCT TCCCATATCC TTCCAGCAAA CTGGAGCATC TCTGGAGGCT GCTGCTCCTG 
AATCAGTTTC ATGACGTGCT GCCGGGCAGC TGCATCCAGC TAGTTGTGGA GGATGCCCTG 
AGGTATTACG CAGAAATCCT TTTGCCGACT GGTCCAGAGA CAGCAGAAAG CCTCCTTGTT 
CTAAACACTT TGCCCTGGGA ACGGACTGAA GTCATCTCCA GGACCGGAGA AGAGACCTTA 
GCCCTGGTGA AGGTGCCTAG CATGGGCTAC TCTGCCGTGA AGGATTCCTT AACACCGCCT 
CATCCCGTGA CAGTGGTGAA GCAGGCGGAT GGCTCTGTTA CCATGGAGAA CGGGGTGATC 
GCAGCTCACC TGGACTCCAT GGGACGCGTG ATATCGCTTC GCTTGGTGCA CTCGGGGAGA 
GAGTCGGTCC AGGATGGCCA CTGTGCTAAC CAGTTTGTGA TATTTGATGA CATTCCCTTG 
TACTGGGATG CTTGGGATGC GATGGATTAT CACCTGGAAA CCAGGAAGCC AATCACATCT 
CTTCTGAAGC CCCTGGAAAT CGTCCTAGCT GGGGGCCTGC GTGGAAGCGT GAAATTCTCT 
CTGCAGATCA GTGAGCGGAG TGTCATCACC CAGGAGGTGA TCCTGGATGC CATGTGTCCT 
TATCTTCGGT TCCTGACCCA GGTGGACTGG AATGAGGCTC ACCGATTCCT GAAGGTGGAG 
TTCCCCTTGC GGGTTCGGAG CATGAATGCA ACGTATGAGA TTCAGTTTGG GCACCTGCAG 
AGGCCGACGC ACTGGAACAC GTCCTGGGAC TGGGCTCGAT TCGAGGTGTG GAGTCACAAG 
TGGATGGACC TCGGGATCAG CTGCCTGCCC ATTCTGCCAG CGCCGCGGGC GTTCGCTCTC 
ATGGCAGGAC TCCCCGCAGC ACTGCCACGG AGCCTGCGGG GCAGGGCGCA GGAGACCCTG 
CTGCGAGACG GGGTGGCAGG GCGGCTTGAC GCTAGCCAGG GCTGTGCACG TGGACTCGCG 
CTTTCAGGGA GCGGGGCTGG GTTTAGTCAC GGCCAGGGGA AGAGGCTCCA CTCCATTAAT 
CCGCCCCTCT CCTCCAGGCT GAGAGCCCCA AAGTCACCTG ATGCCACTGC AGATATTGGG 
CACCATCAGT TCACCTACGC CGTGATGCCC CACAGGGGCT CCTTCCAGGA GGCGGGTGTA 
ATCCATTGTG CCTACAGTTT GAACTACCCT CTGCACACTA TTCCTGTTGT CTCGGTGCCA 
TGTCCAGCCT GGAGCGCCTT CTCCGTGGAC TCCCCAGCTG TGGTGCTAGA AACCGTCAAA 
CAGGCTGAGG ACAGGCTGGA CAGCCTGGTG TTGAGGCTGT ATGAGTCATA TGGCAGCACA 
GTGGTCACAT GGCTCCGGAC TGCCCTTCCC ATCAAGGAAG CAATCGACTG TGACCTCCTG 
GAACGGCCGG ACCCCAGCAG CCATCTGCCA CTGGGGGAGC ATGGCCTAAC ACTCTCCTTT 
ACGCCGTTCC AAGTGCGCTC TCTCCTCCTG ATCTTGAGGC AGTAA