PLD1 cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLD1
Entrez Gene ID	102453139
Full Name	phospholipase D1
Gene Type	protein-coding
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014572375.1	XP_014427861.1	phospholipase D1
XM_014572375.2	XP_014427861.1	phospholipase D1

Pathways from BioSystems
Gene Ontology

The following PLD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPe27228	XM_014572375.2 Latest version!	Pelodiscus sinensis phospholipase D1 (PLD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OPe27228	XM_014572375.1	Pelodiscus sinensis phospholipase D1, phosphatidylcholine-specific (PLD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLD1 ( XM_014572375.1 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_014427861.1 Pelodiscus sinensis phospholipase D1, phosphatidylcholine-specific (PLD1), mRNA.

Price & Availability↑

CloneID	OPe27228
Clone ID Related Accession (Same CDS sequence)	XM_014572375.1 , XM_014572375.2
Accession Version	XM_014572375.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-02
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	phospholipase D1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005854429.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pelodiscus sinensis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTGA CCAGGGAAAG AAGAGTAAAT ACCTCGACGC TGGAGAAAGT TGCAACAGAC 
ATGAGCAATT TAATAGAGAG CCTGGACACG AGGGAGCTCC ACTTTGAGGG GGAAGAGGTG 
GATGCTGACG TATTAAACGA TCCGAAAACA ACTGGGGGCA TCCCATTCTC TGTAATTTAT 
AACACTCAAG GGTTCAAGGA GCCTCATATA CAGACATATC TAATTGGATG TCCGATTAAA 
GTCCGAGTTC TGGAAGTAGA ACGCTTTACC TCCACAAAAA AGGTTCCAAG TCCAAATGTT 
TACACTATTG AGTTCACACA TGGAGAATTT GTATGGCAAG TGAAAAGGAA ATTCAAGCAC 
TTTCAAGAAT TTCACAGGGA CCTGCTGAGA TACAAAGCCT TCATACGAAT TCCTCTCCCT 
ACAAGGAGTC ACACCGTTAG AAGACAAACT ATCAAAAGGG GAGAACCGAG GCAGATGCCA 
AGTCTGCCAC GTACGTCTGA GAACATGGTC CGAGAAGAAC ATTTTTCTAG TAGAAGAAAA 
CAACTGGAAG ACTACCTGAC AAAGATCTTA AAAATGCCCA TGTACAGGAA CTACCATGGA 
ACAACGGAAT TCATTGGTGT AAGTCAGCTG TCATTTATCC GTGATCTAGG ACCAAAGGGC 
ATAGAAGGTA TGATCATGAA AAGATCTGGG GGCCACAGAA TACCCGGTCT GAATTGCTGT 
GGGCAACAAA GGGCCTGCTA TCGATGGTCA AAAAGGTGGT TAGTAGTGAA AGACTCTTTC 
CTAATGTACA TGAAGCCAGA TAGTGGAGCC ATTTCCTTTG TCCTGCTGGC AGATAAAGAA 
TTCAGCATTA AGATTGGCAA GAAAGAGACT GAAACAGAAT ATGGGTTGCA GATTGACAAT 
CTCTCCAGGT CATTGATTTT GAAGTGTAAC AGCTACAGAC ATGCCCGATG GTGGGGACAG 
GGAATAGAAG AATTCATTCA GAAAAATGGC AAAAACTTTC TAACAGATCA CAGATTCGGA 
TCATATGCTG CTGTGCAGGA GAATACTTTG GCAAAGTGGT ATGTAAATGC AAAGGGGTAT 
TTTGAAGATG TAGCAAATGC CATGGAGGCA GCGAAAGAAG AAATTTTCAT CACCGATTGG 
TGGTTGAGCC CAGAAATCTT TATGAAGCGA CCTGTGGTAG AAGGAAATCG CTGGAGACTA 
GACTGCATTC TCAAGCGGAA AGCGCAACAA GGAGTGAGAA TTTTTGTAAT GCTCTACAAA 
GAGGTGGAGC TTGCTCTAGG AATCAACAGT GAATACAGCA AGCGGACATT GATGCGTCTA 
CATCCAAACA TCAAGGTGAT GAGACACCCA GATCACGTGT CATCCTCAGT GTACTTGTGG 
GCCCATCATG AGAAGCTCGT TGTCATTGAC CAGTCTGTAG CATTCGTTGG TGGCATTGAC 
TTGGCCTACG GGAGGTGGGA CGATGACGAA CACAGGCTAA CTGACGTTGG CAGTGTGAAA 
CGAATCACAG GAACAAAGTC CACCTCTATA TTGAATCTCA CACCTACAGC TGAGCATGCT 
GACGCGTTAG CCCCAAAACC TCAGCCTCAG CCCCTGGGAA ACCTGCCACC ACACAGGAAT 
AGTGAAGATA TCCTAGATTC TTCCAGAATG AAGGGAGTAG GAAAACCCAA AAAGTTTTCC 
AGGTTCAGCA TTTATAAGCA TCTCCAGAAG CACGGTCTAC ACCATGCTGA TAGCGTGAGC 
AGCATAGACA GCGAGTCAGG TTATTGTAAT CCTAGTAGAA GCCAGCAGAA TTTAATCCAG 
AGTTTAAAGC CCCATCTGAA AATATTTCAT CATCCCACTG AGTCCAAACA AGGGCTCACT 
GAGTATGCAG ATGATAAAGG ATCAACGCAC AGCTTGAAGA CAGGTGTTGG AGAACTTCTC 
GGGGAGACCA GATTCTGGCA TGGAAAGGAC TATTGTAATT TTGTCTTTAA AGACTGGGTT 
CAACTTGACA AACCCTTTGC TGATTTCATT GATAGATACA CCACACCCAG AATGCCATGG 
CATGACATTT CATCCGTTGT GCATGGCAAA GCTGCGCGGG ATGTTGCACG ACACTTTATT 
CAGCGCTGGA ACTTCACCAA GATCATGAAG CCCAAATACA GATCCCTGTC CTACCCATTC 
CTGTTGCCAA AATCTCAGAG AACCGCCAAT GAGATGAAGT ATCAAGTACC TGACTCTGTA 
TGTGCCAATG TACAGGTGCT AAGTTCTGCT GCAGATTGGT CAGCTGGTAT CAAGTACCAT 
GAAGAATCCA TCCATAACGC TTATGTTAGC GTGATAGAAA ACAGCAAACA CTATATATAT 
ATAGAAAACC AATTTTTTAT CAGCTGTGCT GACGACAAAG TTGTTTCTAA CAAGATTGGC 
GATACCATTG CTCAGAGGAT TCTTAAAGCA CACAGGGAAA ATAAACGATA CCGAGTGTAT 
GTGGTGATCC CGCTGCTGCC TGGATTTGAA GGAGATATTT CAACTGGAGG TGGGAATGCG 
CTGCAGGCCA TAATGCATTT CAACTACAGG ACCATGTGCA GAGGAGAGAA TTCCATCCTA 
GGACAGCTGA AAGCAGAGAT TGGGGATAAG TGGATAAACT ACATCTCATT CTGTGGGCTT 
CGAACATACG CGGAACTGGA AGGAAATCTT GTCACAGAAC TCATCTATGT TCACAGCAAG 
TTGCTTATTG CCGATGACGA CACCGTCATC ATCGGCTCTG CTAACATCAA TGACCGCAGC 
ATGCTGGGAA AACGTGACAG TGAAATGGCT ATCATTGTGC AAGACGCCAA AACCATCCCG 
TCCGTGATGG ATGGAAAGGA CTACAAGGCT GGACAATTTG CTCAGTCACT CCGCCTCCGG 
TGTTTTAGGG TAGTGCTTGG TTGTTCAGCA GATCTCAGTA CAGATCTCCA GGATCCTGTT 
TGTGACAAAT TCTTCAAGGA GGTGTGGGTT TCAACAGCTG CCCGCAATGC CACTATTTTT 
GATAAGGTTT TCCGATGCCT TCCCAATGAC GAGGTGAATA ATTTAGCCCA GCTGCGTGAT 
TTCATCACCA AGTCAAAATT GGTAACCACA GATGCTGCAA AAGCAGCAGA AGAATTAAAG 
AAGATTCATG GATTTCTAGT GCAATTCCCC TTTCGTTTCC TGCACGAAGA AAACTTGCTT 
CCTTCTGTTG GAACAAAAGA ATCCATGGTG CCCATGGAAG TTTGGACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014427861.1
CDS	194..3424
Translation	MSLTRERRVNTSTLEKVATDMSNLIESLDTRELHFEGEEVDADVLNDPKTTGGIPFSVIYNTQGFKEPHIQTYLIGCPIKVRVLEVERFTSTKKVPSPNVYTIEFTHGEFVWQVKRKFKHFQEFHRDLLRYKAFIRIPLPTRSHTVRRQTIKRGEPRQMPSLPRTSENMVREEHFSSRRKQLEDYLTKILKMPMYRNYHGTTEFIGVSQLSFIRDLGPKGIEGMIMKRSGGHRIPGLNCCGQQRACYRWSKRWLVVKDSFLMYMKPDSGAISFVLLADKEFSIKIGKKETETEYGLQIDNLSRSLILKCNSYRHARWWGQGIEEFIQKNGKNFLTDHRFGSYAAVQENTLAKWYVNAKGYFEDVANAMEAAKEEIFITDWWLSPEIFMKRPVVEGNRWRLDCILKRKAQQGVRIFVMLYKEVELALGINSEYSKRTLMRLHPNIKVMRHPDHVSSSVYLWAHHEKLVVIDQSVAFVGGIDLAYGRWDDDEHRLTDVGSVKRITGTKSTSILNLTPTAEHADALAPKPQPQPLGNLPPHRNSEDILDSSRMKGVGKPKKFSRFSIYKHLQKHGLHHADSVSSIDSESGYCNPSRSQQNLIQSLKPHLKIFHHPTESKQGLTEYADDKGSTHSLKTGVGELLGETRFWHGKDYCNFVFKDWVQLDKPFADFIDRYTTPRMPWHDISSVVHGKAARDVARHFIQRWNFTKIMKPKYRSLSYPFLLPKSQRTANEMKYQVPDSVCANVQVLSSAADWSAGIKYHEESIHNAYVSVIENSKHYIYIENQFFISCADDKVVSNKIGDTIAQRILKAHRENKRYRVYVVIPLLPGFEGDISTGGGNALQAIMHFNYRTMCRGENSILGQLKAEIGDKWINYISFCGLRTYAELEGNLVTELIYVHSKLLIADDDTVIIGSANINDRSMLGKRDSEMAIIVQDAKTIPSVMDGKDYKAGQFAQSLRLRCFRVVLGCSADLSTDLQDPVCDKFFKEVWVSTAARNATIFDKVFRCLPNDEVNNLAQLRDFITKSKLVTTDAAKAAEELKKIHGFLVQFPFRFLHEENLLPSVGTKESMVPMEVWT

Target ORF information:

RefSeq Version	XM_014572375.1
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis phospholipase D1, phosphatidylcholine-specific (PLD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014572375.1

ORF Insert Sequence:

ATGTCACTGA CCAGGGAAAG AAGAGTAAAT ACCTCGACGC TGGAGAAAGT TGCAACAGAC 
ATGAGCAATT TAATAGAGAG CCTGGACACG AGGGAGCTCC ACTTTGAGGG GGAAGAGGTG 
GATGCTGACG TATTAAACGA TCCGAAAACA ACTGGGGGCA TCCCATTCTC TGTAATTTAT 
AACACTCAAG GGTTCAAGGA GCCTCATATA CAGACATATC TAATTGGATG TCCGATTAAA 
GTCCGAGTTC TGGAAGTAGA ACGCTTTACC TCCACAAAAA AGGTTCCAAG TCCAAATGTT 
TACACTATTG AGTTCACACA TGGAGAATTT GTATGGCAAG TGAAAAGGAA ATTCAAGCAC 
TTTCAAGAAT TTCACAGGGA CCTGCTGAGA TACAAAGCCT TCATACGAAT TCCTCTCCCT 
ACAAGGAGTC ACACCGTTAG AAGACAAACT ATCAAAAGGG GAGAACCGAG GCAGATGCCA 
AGTCTGCCAC GTACGTCTGA GAACATGGTC CGAGAAGAAC ATTTTTCTAG TAGAAGAAAA 
CAACTGGAAG ACTACCTGAC AAAGATCTTA AAAATGCCCA TGTACAGGAA CTACCATGGA 
ACAACGGAAT TCATTGGTGT AAGTCAGCTG TCATTTATCC GTGATCTAGG ACCAAAGGGC 
ATAGAAGGTA TGATCATGAA AAGATCTGGG GGCCACAGAA TACCCGGTCT GAATTGCTGT 
GGGCAACAAA GGGCCTGCTA TCGATGGTCA AAAAGGTGGT TAGTAGTGAA AGACTCTTTC 
CTAATGTACA TGAAGCCAGA TAGTGGAGCC ATTTCCTTTG TCCTGCTGGC AGATAAAGAA 
TTCAGCATTA AGATTGGCAA GAAAGAGACT GAAACAGAAT ATGGGTTGCA GATTGACAAT 
CTCTCCAGGT CATTGATTTT GAAGTGTAAC AGCTACAGAC ATGCCCGATG GTGGGGACAG 
GGAATAGAAG AATTCATTCA GAAAAATGGC AAAAACTTTC TAACAGATCA CAGATTCGGA 
TCATATGCTG CTGTGCAGGA GAATACTTTG GCAAAGTGGT ATGTAAATGC AAAGGGGTAT 
TTTGAAGATG TAGCAAATGC CATGGAGGCA GCGAAAGAAG AAATTTTCAT CACCGATTGG 
TGGTTGAGCC CAGAAATCTT TATGAAGCGA CCTGTGGTAG AAGGAAATCG CTGGAGACTA 
GACTGCATTC TCAAGCGGAA AGCGCAACAA GGAGTGAGAA TTTTTGTAAT GCTCTACAAA 
GAGGTGGAGC TTGCTCTAGG AATCAACAGT GAATACAGCA AGCGGACATT GATGCGTCTA 
CATCCAAACA TCAAGGTGAT GAGACACCCA GATCACGTGT CATCCTCAGT GTACTTGTGG 
GCCCATCATG AGAAGCTCGT TGTCATTGAC CAGTCTGTAG CATTCGTTGG TGGCATTGAC 
TTGGCCTACG GGAGGTGGGA CGATGACGAA CACAGGCTAA CTGACGTTGG CAGTGTGAAA 
CGAATCACAG GAACAAAGTC CACCTCTATA TTGAATCTCA CACCTACAGC TGAGCATGCT 
GACGCGTTAG CCCCAAAACC TCAGCCTCAG CCCCTGGGAA ACCTGCCACC ACACAGGAAT 
AGTGAAGATA TCCTAGATTC TTCCAGAATG AAGGGAGTAG GAAAACCCAA AAAGTTTTCC 
AGGTTCAGCA TTTATAAGCA TCTCCAGAAG CACGGTCTAC ACCATGCTGA TAGCGTGAGC 
AGCATAGACA GCGAGTCAGG TTATTGTAAT CCTAGTAGAA GCCAGCAGAA TTTAATCCAG 
AGTTTAAAGC CCCATCTGAA AATATTTCAT CATCCCACTG AGTCCAAACA AGGGCTCACT 
GAGTATGCAG ATGATAAAGG ATCAACGCAC AGCTTGAAGA CAGGTGTTGG AGAACTTCTC 
GGGGAGACCA GATTCTGGCA TGGAAAGGAC TATTGTAATT TTGTCTTTAA AGACTGGGTT 
CAACTTGACA AACCCTTTGC TGATTTCATT GATAGATACA CCACACCCAG AATGCCATGG 
CATGACATTT CATCCGTTGT GCATGGCAAA GCTGCGCGGG ATGTTGCACG ACACTTTATT 
CAGCGCTGGA ACTTCACCAA GATCATGAAG CCCAAATACA GATCCCTGTC CTACCCATTC 
CTGTTGCCAA AATCTCAGAG AACCGCCAAT GAGATGAAGT ATCAAGTACC TGACTCTGTA 
TGTGCCAATG TACAGGTGCT AAGTTCTGCT GCAGATTGGT CAGCTGGTAT CAAGTACCAT 
GAAGAATCCA TCCATAACGC TTATGTTAGC GTGATAGAAA ACAGCAAACA CTATATATAT 
ATAGAAAACC AATTTTTTAT CAGCTGTGCT GACGACAAAG TTGTTTCTAA CAAGATTGGC 
GATACCATTG CTCAGAGGAT TCTTAAAGCA CACAGGGAAA ATAAACGATA CCGAGTGTAT 
GTGGTGATCC CGCTGCTGCC TGGATTTGAA GGAGATATTT CAACTGGAGG TGGGAATGCG 
CTGCAGGCCA TAATGCATTT CAACTACAGG ACCATGTGCA GAGGAGAGAA TTCCATCCTA 
GGACAGCTGA AAGCAGAGAT TGGGGATAAG TGGATAAACT ACATCTCATT CTGTGGGCTT 
CGAACATACG CGGAACTGGA AGGAAATCTT GTCACAGAAC TCATCTATGT TCACAGCAAG 
TTGCTTATTG CCGATGACGA CACCGTCATC ATCGGCTCTG CTAACATCAA TGACCGCAGC 
ATGCTGGGAA AACGTGACAG TGAAATGGCT ATCATTGTGC AAGACGCCAA AACCATCCCG 
TCCGTGATGG ATGGAAAGGA CTACAAGGCT GGACAATTTG CTCAGTCACT CCGCCTCCGG 
TGTTTTAGGG TAGTGCTTGG TTGTTCAGCA GATCTCAGTA CAGATCTCCA GGATCCTGTT 
TGTGACAAAT TCTTCAAGGA GGTGTGGGTT TCAACAGCTG CCCGCAATGC CACTATTTTT 
GATAAGGTTT TCCGATGCCT TCCCAATGAC GAGGTGAATA ATTTAGCCCA GCTGCGTGAT 
TTCATCACCA AGTCAAAATT GGTAACCACA GATGCTGCAA AAGCAGCAGA AGAATTAAAG 
AAGATTCATG GATTTCTAGT GCAATTCCCC TTTCGTTTCC TGCACGAAGA AAACTTGCTT 
CCTTCTGTTG GAACAAAAGA ATCCATGGTG CCCATGGAAG TTTGGACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLD1 ( XM_014572375.2 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_014427861.1 Pelodiscus sinensis phospholipase D1 (PLD1), mRNA.

Price & Availability↑

CloneID	OPe27228
Clone ID Related Accession (Same CDS sequence)	XM_014572375.1 , XM_014572375.2
Accession Version	XM_014572375.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	phospholipase D1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005854429.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 4, 2018 this sequence version replaced XM_014572375.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pelodiscus sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTGA CCAGGGAAAG AAGAGTAAAT ACCTCGACGC TGGAGAAAGT TGCAACAGAC 
ATGAGCAATT TAATAGAGAG CCTGGACACG AGGGAGCTCC ACTTTGAGGG GGAAGAGGTG 
GATGCTGACG TATTAAACGA TCCGAAAACA ACTGGGGGCA TCCCATTCTC TGTAATTTAT 
AACACTCAAG GGTTCAAGGA GCCTCATATA CAGACATATC TAATTGGATG TCCGATTAAA 
GTCCGAGTTC TGGAAGTAGA ACGCTTTACC TCCACAAAAA AGGTTCCAAG TCCAAATGTT 
TACACTATTG AGTTCACACA TGGAGAATTT GTATGGCAAG TGAAAAGGAA ATTCAAGCAC 
TTTCAAGAAT TTCACAGGGA CCTGCTGAGA TACAAAGCCT TCATACGAAT TCCTCTCCCT 
ACAAGGAGTC ACACCGTTAG AAGACAAACT ATCAAAAGGG GAGAACCGAG GCAGATGCCA 
AGTCTGCCAC GTACGTCTGA GAACATGGTC CGAGAAGAAC ATTTTTCTAG TAGAAGAAAA 
CAACTGGAAG ACTACCTGAC AAAGATCTTA AAAATGCCCA TGTACAGGAA CTACCATGGA 
ACAACGGAAT TCATTGGTGT AAGTCAGCTG TCATTTATCC GTGATCTAGG ACCAAAGGGC 
ATAGAAGGTA TGATCATGAA AAGATCTGGG GGCCACAGAA TACCCGGTCT GAATTGCTGT 
GGGCAACAAA GGGCCTGCTA TCGATGGTCA AAAAGGTGGT TAGTAGTGAA AGACTCTTTC 
CTAATGTACA TGAAGCCAGA TAGTGGAGCC ATTTCCTTTG TCCTGCTGGC AGATAAAGAA 
TTCAGCATTA AGATTGGCAA GAAAGAGACT GAAACAGAAT ATGGGTTGCA GATTGACAAT 
CTCTCCAGGT CATTGATTTT GAAGTGTAAC AGCTACAGAC ATGCCCGATG GTGGGGACAG 
GGAATAGAAG AATTCATTCA GAAAAATGGC AAAAACTTTC TAACAGATCA CAGATTCGGA 
TCATATGCTG CTGTGCAGGA GAATACTTTG GCAAAGTGGT ATGTAAATGC AAAGGGGTAT 
TTTGAAGATG TAGCAAATGC CATGGAGGCA GCGAAAGAAG AAATTTTCAT CACCGATTGG 
TGGTTGAGCC CAGAAATCTT TATGAAGCGA CCTGTGGTAG AAGGAAATCG CTGGAGACTA 
GACTGCATTC TCAAGCGGAA AGCGCAACAA GGAGTGAGAA TTTTTGTAAT GCTCTACAAA 
GAGGTGGAGC TTGCTCTAGG AATCAACAGT GAATACAGCA AGCGGACATT GATGCGTCTA 
CATCCAAACA TCAAGGTGAT GAGACACCCA GATCACGTGT CATCCTCAGT GTACTTGTGG 
GCCCATCATG AGAAGCTCGT TGTCATTGAC CAGTCTGTAG CATTCGTTGG TGGCATTGAC 
TTGGCCTACG GGAGGTGGGA CGATGACGAA CACAGGCTAA CTGACGTTGG CAGTGTGAAA 
CGAATCACAG GAACAAAGTC CACCTCTATA TTGAATCTCA CACCTACAGC TGAGCATGCT 
GACGCGTTAG CCCCAAAACC TCAGCCTCAG CCCCTGGGAA ACCTGCCACC ACACAGGAAT 
AGTGAAGATA TCCTAGATTC TTCCAGAATG AAGGGAGTAG GAAAACCCAA AAAGTTTTCC 
AGGTTCAGCA TTTATAAGCA TCTCCAGAAG CACGGTCTAC ACCATGCTGA TAGCGTGAGC 
AGCATAGACA GCGAGTCAGG TTATTGTAAT CCTAGTAGAA GCCAGCAGAA TTTAATCCAG 
AGTTTAAAGC CCCATCTGAA AATATTTCAT CATCCCACTG AGTCCAAACA AGGGCTCACT 
GAGTATGCAG ATGATAAAGG ATCAACGCAC AGCTTGAAGA CAGGTGTTGG AGAACTTCTC 
GGGGAGACCA GATTCTGGCA TGGAAAGGAC TATTGTAATT TTGTCTTTAA AGACTGGGTT 
CAACTTGACA AACCCTTTGC TGATTTCATT GATAGATACA CCACACCCAG AATGCCATGG 
CATGACATTT CATCCGTTGT GCATGGCAAA GCTGCGCGGG ATGTTGCACG ACACTTTATT 
CAGCGCTGGA ACTTCACCAA GATCATGAAG CCCAAATACA GATCCCTGTC CTACCCATTC 
CTGTTGCCAA AATCTCAGAG AACCGCCAAT GAGATGAAGT ATCAAGTACC TGACTCTGTA 
TGTGCCAATG TACAGGTGCT AAGTTCTGCT GCAGATTGGT CAGCTGGTAT CAAGTACCAT 
GAAGAATCCA TCCATAACGC TTATGTTAGC GTGATAGAAA ACAGCAAACA CTATATATAT 
ATAGAAAACC AATTTTTTAT CAGCTGTGCT GACGACAAAG TTGTTTCTAA CAAGATTGGC 
GATACCATTG CTCAGAGGAT TCTTAAAGCA CACAGGGAAA ATAAACGATA CCGAGTGTAT 
GTGGTGATCC CGCTGCTGCC TGGATTTGAA GGAGATATTT CAACTGGAGG TGGGAATGCG 
CTGCAGGCCA TAATGCATTT CAACTACAGG ACCATGTGCA GAGGAGAGAA TTCCATCCTA 
GGACAGCTGA AAGCAGAGAT TGGGGATAAG TGGATAAACT ACATCTCATT CTGTGGGCTT 
CGAACATACG CGGAACTGGA AGGAAATCTT GTCACAGAAC TCATCTATGT TCACAGCAAG 
TTGCTTATTG CCGATGACGA CACCGTCATC ATCGGCTCTG CTAACATCAA TGACCGCAGC 
ATGCTGGGAA AACGTGACAG TGAAATGGCT ATCATTGTGC AAGACGCCAA AACCATCCCG 
TCCGTGATGG ATGGAAAGGA CTACAAGGCT GGACAATTTG CTCAGTCACT CCGCCTCCGG 
TGTTTTAGGG TAGTGCTTGG TTGTTCAGCA GATCTCAGTA CAGATCTCCA GGATCCTGTT 
TGTGACAAAT TCTTCAAGGA GGTGTGGGTT TCAACAGCTG CCCGCAATGC CACTATTTTT 
GATAAGGTTT TCCGATGCCT TCCCAATGAC GAGGTGAATA ATTTAGCCCA GCTGCGTGAT 
TTCATCACCA AGTCAAAATT GGTAACCACA GATGCTGCAA AAGCAGCAGA AGAATTAAAG 
AAGATTCATG GATTTCTAGT GCAATTCCCC TTTCGTTTCC TGCACGAAGA AAACTTGCTT 
CCTTCTGTTG GAACAAAAGA ATCCATGGTG CCCATGGAAG TTTGGACTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014427861.1
CDS	197..3427
Translation	MSLTRERRVNTSTLEKVATDMSNLIESLDTRELHFEGEEVDADVLNDPKTTGGIPFSVIYNTQGFKEPHIQTYLIGCPIKVRVLEVERFTSTKKVPSPNVYTIEFTHGEFVWQVKRKFKHFQEFHRDLLRYKAFIRIPLPTRSHTVRRQTIKRGEPRQMPSLPRTSENMVREEHFSSRRKQLEDYLTKILKMPMYRNYHGTTEFIGVSQLSFIRDLGPKGIEGMIMKRSGGHRIPGLNCCGQQRACYRWSKRWLVVKDSFLMYMKPDSGAISFVLLADKEFSIKIGKKETETEYGLQIDNLSRSLILKCNSYRHARWWGQGIEEFIQKNGKNFLTDHRFGSYAAVQENTLAKWYVNAKGYFEDVANAMEAAKEEIFITDWWLSPEIFMKRPVVEGNRWRLDCILKRKAQQGVRIFVMLYKEVELALGINSEYSKRTLMRLHPNIKVMRHPDHVSSSVYLWAHHEKLVVIDQSVAFVGGIDLAYGRWDDDEHRLTDVGSVKRITGTKSTSILNLTPTAEHADALAPKPQPQPLGNLPPHRNSEDILDSSRMKGVGKPKKFSRFSIYKHLQKHGLHHADSVSSIDSESGYCNPSRSQQNLIQSLKPHLKIFHHPTESKQGLTEYADDKGSTHSLKTGVGELLGETRFWHGKDYCNFVFKDWVQLDKPFADFIDRYTTPRMPWHDISSVVHGKAARDVARHFIQRWNFTKIMKPKYRSLSYPFLLPKSQRTANEMKYQVPDSVCANVQVLSSAADWSAGIKYHEESIHNAYVSVIENSKHYIYIENQFFISCADDKVVSNKIGDTIAQRILKAHRENKRYRVYVVIPLLPGFEGDISTGGGNALQAIMHFNYRTMCRGENSILGQLKAEIGDKWINYISFCGLRTYAELEGNLVTELIYVHSKLLIADDDTVIIGSANINDRSMLGKRDSEMAIIVQDAKTIPSVMDGKDYKAGQFAQSLRLRCFRVVLGCSADLSTDLQDPVCDKFFKEVWVSTAARNATIFDKVFRCLPNDEVNNLAQLRDFITKSKLVTTDAAKAAEELKKIHGFLVQFPFRFLHEENLLPSVGTKESMVPMEVWT

Target ORF information:

RefSeq Version	XM_014572375.2
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis phospholipase D1 (PLD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014572375.2

ORF Insert Sequence:

ATGTCACTGA CCAGGGAAAG AAGAGTAAAT ACCTCGACGC TGGAGAAAGT TGCAACAGAC 
ATGAGCAATT TAATAGAGAG CCTGGACACG AGGGAGCTCC ACTTTGAGGG GGAAGAGGTG 
GATGCTGACG TATTAAACGA TCCGAAAACA ACTGGGGGCA TCCCATTCTC TGTAATTTAT 
AACACTCAAG GGTTCAAGGA GCCTCATATA CAGACATATC TAATTGGATG TCCGATTAAA 
GTCCGAGTTC TGGAAGTAGA ACGCTTTACC TCCACAAAAA AGGTTCCAAG TCCAAATGTT 
TACACTATTG AGTTCACACA TGGAGAATTT GTATGGCAAG TGAAAAGGAA ATTCAAGCAC 
TTTCAAGAAT TTCACAGGGA CCTGCTGAGA TACAAAGCCT TCATACGAAT TCCTCTCCCT 
ACAAGGAGTC ACACCGTTAG AAGACAAACT ATCAAAAGGG GAGAACCGAG GCAGATGCCA 
AGTCTGCCAC GTACGTCTGA GAACATGGTC CGAGAAGAAC ATTTTTCTAG TAGAAGAAAA 
CAACTGGAAG ACTACCTGAC AAAGATCTTA AAAATGCCCA TGTACAGGAA CTACCATGGA 
ACAACGGAAT TCATTGGTGT AAGTCAGCTG TCATTTATCC GTGATCTAGG ACCAAAGGGC 
ATAGAAGGTA TGATCATGAA AAGATCTGGG GGCCACAGAA TACCCGGTCT GAATTGCTGT 
GGGCAACAAA GGGCCTGCTA TCGATGGTCA AAAAGGTGGT TAGTAGTGAA AGACTCTTTC 
CTAATGTACA TGAAGCCAGA TAGTGGAGCC ATTTCCTTTG TCCTGCTGGC AGATAAAGAA 
TTCAGCATTA AGATTGGCAA GAAAGAGACT GAAACAGAAT ATGGGTTGCA GATTGACAAT 
CTCTCCAGGT CATTGATTTT GAAGTGTAAC AGCTACAGAC ATGCCCGATG GTGGGGACAG 
GGAATAGAAG AATTCATTCA GAAAAATGGC AAAAACTTTC TAACAGATCA CAGATTCGGA 
TCATATGCTG CTGTGCAGGA GAATACTTTG GCAAAGTGGT ATGTAAATGC AAAGGGGTAT 
TTTGAAGATG TAGCAAATGC CATGGAGGCA GCGAAAGAAG AAATTTTCAT CACCGATTGG 
TGGTTGAGCC CAGAAATCTT TATGAAGCGA CCTGTGGTAG AAGGAAATCG CTGGAGACTA 
GACTGCATTC TCAAGCGGAA AGCGCAACAA GGAGTGAGAA TTTTTGTAAT GCTCTACAAA 
GAGGTGGAGC TTGCTCTAGG AATCAACAGT GAATACAGCA AGCGGACATT GATGCGTCTA 
CATCCAAACA TCAAGGTGAT GAGACACCCA GATCACGTGT CATCCTCAGT GTACTTGTGG 
GCCCATCATG AGAAGCTCGT TGTCATTGAC CAGTCTGTAG CATTCGTTGG TGGCATTGAC 
TTGGCCTACG GGAGGTGGGA CGATGACGAA CACAGGCTAA CTGACGTTGG CAGTGTGAAA 
CGAATCACAG GAACAAAGTC CACCTCTATA TTGAATCTCA CACCTACAGC TGAGCATGCT 
GACGCGTTAG CCCCAAAACC TCAGCCTCAG CCCCTGGGAA ACCTGCCACC ACACAGGAAT 
AGTGAAGATA TCCTAGATTC TTCCAGAATG AAGGGAGTAG GAAAACCCAA AAAGTTTTCC 
AGGTTCAGCA TTTATAAGCA TCTCCAGAAG CACGGTCTAC ACCATGCTGA TAGCGTGAGC 
AGCATAGACA GCGAGTCAGG TTATTGTAAT CCTAGTAGAA GCCAGCAGAA TTTAATCCAG 
AGTTTAAAGC CCCATCTGAA AATATTTCAT CATCCCACTG AGTCCAAACA AGGGCTCACT 
GAGTATGCAG ATGATAAAGG ATCAACGCAC AGCTTGAAGA CAGGTGTTGG AGAACTTCTC 
GGGGAGACCA GATTCTGGCA TGGAAAGGAC TATTGTAATT TTGTCTTTAA AGACTGGGTT 
CAACTTGACA AACCCTTTGC TGATTTCATT GATAGATACA CCACACCCAG AATGCCATGG 
CATGACATTT CATCCGTTGT GCATGGCAAA GCTGCGCGGG ATGTTGCACG ACACTTTATT 
CAGCGCTGGA ACTTCACCAA GATCATGAAG CCCAAATACA GATCCCTGTC CTACCCATTC 
CTGTTGCCAA AATCTCAGAG AACCGCCAAT GAGATGAAGT ATCAAGTACC TGACTCTGTA 
TGTGCCAATG TACAGGTGCT AAGTTCTGCT GCAGATTGGT CAGCTGGTAT CAAGTACCAT 
GAAGAATCCA TCCATAACGC TTATGTTAGC GTGATAGAAA ACAGCAAACA CTATATATAT 
ATAGAAAACC AATTTTTTAT CAGCTGTGCT GACGACAAAG TTGTTTCTAA CAAGATTGGC 
GATACCATTG CTCAGAGGAT TCTTAAAGCA CACAGGGAAA ATAAACGATA CCGAGTGTAT 
GTGGTGATCC CGCTGCTGCC TGGATTTGAA GGAGATATTT CAACTGGAGG TGGGAATGCG 
CTGCAGGCCA TAATGCATTT CAACTACAGG ACCATGTGCA GAGGAGAGAA TTCCATCCTA 
GGACAGCTGA AAGCAGAGAT TGGGGATAAG TGGATAAACT ACATCTCATT CTGTGGGCTT 
CGAACATACG CGGAACTGGA AGGAAATCTT GTCACAGAAC TCATCTATGT TCACAGCAAG 
TTGCTTATTG CCGATGACGA CACCGTCATC ATCGGCTCTG CTAACATCAA TGACCGCAGC 
ATGCTGGGAA AACGTGACAG TGAAATGGCT ATCATTGTGC AAGACGCCAA AACCATCCCG 
TCCGTGATGG ATGGAAAGGA CTACAAGGCT GGACAATTTG CTCAGTCACT CCGCCTCCGG 
TGTTTTAGGG TAGTGCTTGG TTGTTCAGCA GATCTCAGTA CAGATCTCCA GGATCCTGTT 
TGTGACAAAT TCTTCAAGGA GGTGTGGGTT TCAACAGCTG CCCGCAATGC CACTATTTTT 
GATAAGGTTT TCCGATGCCT TCCCAATGAC GAGGTGAATA ATTTAGCCCA GCTGCGTGAT 
TTCATCACCA AGTCAAAATT GGTAACCACA GATGCTGCAA AAGCAGCAGA AGAATTAAAG 
AAGATTCATG GATTTCTAGT GCAATTCCCC TTTCGTTTCC TGCACGAAGA AAACTTGCTT 
CCTTCTGTTG GAACAAAAGA ATCCATGGTG CCCATGGAAG TTTGGACTTA G