SMO cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SMO
Entrez Gene ID	102449517
Full Name	smoothened, frizzled class receptor
Gene Type	protein-coding
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014572119.1	XP_014427605.1	smoothened homolog
XM_025183206.1	XP_025038991.1	smoothened homolog

Pathways from BioSystems
Gene Ontology

The following SMO gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMO cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPe149818	XM_025183206.1 Latest version!	Pelodiscus sinensis smoothened, frizzled class receptor (SMO), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OPe26765	XM_014572119.1 Latest version!	Pelodiscus sinensis smoothened, frizzled class receptor (SMO), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SMO ( XM_025183206.1 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_025038991.1 Pelodiscus sinensis smoothened, frizzled class receptor (SMO), mRNA.

Price & Availability↑

CloneID	OPe149818
Clone ID Related Accession (Same CDS sequence)	XM_025183206.1
Accession Version	XM_025183206.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	smoothened homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005854397.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pelodiscus sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 20% of CDS bases ##RefSeq-Attributes-END##

ATGTCACGCC CGTGCTCTGA GCACTTGGGG ACGTTCTCAA TCTGCTTGAC CATCATCTTC 
CTGCTGCAGC TGGCAGCTGG AGTACTGGGC TTTGTCTTCT CGGATAAGGC ACGCGGGAAG 
GTGAGCGAGA TCATCAACAA CGCCATCGTG CATTACCGGG ACGACCTGGA CCTGCAGAAC 
CTCATCGACT TCGGCCAGAA GGAGTTCAGT TGCTGTGGGG GTGTCTCCTA CAAGGACTGG 
TCCCAAAACA TGTATTTCAA CTGCACTGCT GACAACCCCA GCCGGGAGCG CTGTTCCGTG 
CCTTATTCTT GCTGCCTGCA CGCTGCCGAC CAGCCAAAAT ATTGGACTCC TCCGCCACAT 
GCCCTGCGCC ATTCAGAAAA AGAGAAGTCA GGACCACCTG GGGAATTCCA GAGCTTCAGT 
ACCCAGGACG ATAATGGAGC AAACCAATCA GCCTTCAATT TGGGTGTTGT CTTTCTTTGC 
CTCCTCCGTG GCAATTTGCT GACCGCTGTT TTCCCTCCCC GGCAGTTCTC AATCTGCTTG 
ACCATCATCT TCCTGCTGCA GCTGGCAGCT GGAGTACTGG GCTTTGTCTT CTCGGATAAG 
GCACGCGGGA AGGTGAGCGA GATCATCAAC AACGCCATCG TGCATTACCG GGACGACCTG 
GACCTGCAGA ACCTCATCGA CTTCGGCCAG AAGGAGTTCA GTTGCTGTGG GGGTGTCTCC 
TACAAGGACT GGTCCCAAAA CATGTATTTC AACTGCACTG CTGACAACCC CAGCCGGGAG 
CGCTGTTCCG TGCCTTATTC TTGCTGCCTG CACGCTGCCG ACCAGGCCGT CATCAACACC 
ATGTGTGGCC AGGGCATGCA AGCCATGAAC TACCTGGAGG CCAGCGAGTT CATCTACCCC 
AACGGCTGCA TCGACAAGCT GCTGCTCTGG CCAGGCTTGC GGAACGCCCC GCGCTGCTGG 
GACGCCATCC AGCCGCTGCT CTGCGCCGTG TACATGCCCA AGTGCGAGGA CGGCCAGGTG 
GAGCTGCCCA GCCAGACCTT GTGCCAGGCC ACCCGGGGCC CGTGCACCAT CGTGGAACGG 
GAGCGGGGCT GGCCCGACTT CCTCAAGTGC ACCACGGACC GCTTTCCGGA AGGCTGCCCG 
AACGAGGTGC AGAACATCAA ATTCAACAGC TCGGGGCACT GCGAGGCGCC CCTGGTGCAG 
ACGGACAACC CCAAGAGCTG GTACGAGGAC GTGGAGGGCT GCGGCATCCA GTGCCAGAAC 
CCCCTCTTCA CGGAGAGCGA GCACCGGGAC ATGCACGTGT ACATCGCCGC CTTCAGCTCC 
GTCACCATCT TCTGCACCTT CTTCACCCTG GCCACGTTCG TTGCCGACTG GAAGAACTCG 
AACCGTTACC CGGCCGTCAT CCTCTTCTAC GTCAACGCCT GCTTCTTTGT GGGCAGCATT 
GGCTGGCTGG CGCAGTTCAT GGACGGGGCC CGCGGCGAGA TCGTGTGCCG AGCCGACNNN 
NNNNNNNNNN NNNNNNTCCC CAGCTCCAAC GAGACGCTCT CCTGCGTGAT CATCTTCGTC 
ATCGTCTACT ACTCGCTGAT GTCGGGCGTG ATCTGGTTCG TCATGCTGAC CTACGCCTGG 
CACACCTCGT TCAAGGCCCT GGGCACCACC TACCAGCCGC TGCTGGGCAA GACCTCCTAC 
TTCCACCTGG TCACCTGGGC CATCCCTTTC GTCCTCACCG TCGCCATCCT GGCTGTCGCC 
CAGGTGGATG GAGACTCCGT CAGCGGCATC TGCTTTGTGG GCTACAAGAA CTATCGCTAC 
CGGGCCGGCT TCGTCCTGGC GCCCATCGGC CTGGTGCTGA TCGTGGGAGG CTACTTCCTC 
ATCCGAGGCG TCATGACGTT GTTCTCCATC AAAAGTAACC ACCCCGGCCT GCTGAGCGAG 
AAGGCGGCCA GCAAAATCAA CGAGACCATG TTACGGCTGG GTAAGCGGGG CCCGGGCGGG 
CCTGGCTTGT GGGGGACCGC TGGTGTCCAT GGGAAGCTCT GTGGTGCGCG AGAAGTGGGT 
GCCGCTGGTG TTCACGAGGA CCCTAGAGGT GTCGAAGGAG CCCGAGCTAT GCTTTTCATC 
CACACCAACA AGCCCATCCC CAACTGCGAG ATCAAGAACC GGCCCAGCCT GCTGGTGGAG 
AAGATCAACC TCTTCGCCAT GTTCGGCACC GGCATCTCCA TGAGCACCTG GGTGTGGACC 
AAAGCCACCC TCCTCATCTG GAAGCGCACC TGGTGCAGGC TGACGGGACA GAGCGATGAC 
GAGCCCAAGA GGATCAAGAA AAGCAAGATG ATTGCCAAGG CCTTCTCCCG GCGGAAGGAG 
CTGCTGCACA ACCCGGAGCA GGAGCTGTCC CCTCTTAATG AGAGCCGCTT TGTCCAGCGA 
CGACAACAGT TTTTACTCGC TGTTCCTCCT CTCCGCTGGG AGCCAGATTC AAGGCCGGCT 
CCTGGTTTGG CTTTCGACCT CAACGAGCCG TCAGCCGACG TGTCCTCGGC CTGGGCCCAG 
CACGTCACCA AGATGGTGGC CAGGAGAGGA GCCATCCTGC CTCAGGACAT CTCGGTCACA 
CCCGTGGCGA CGCCTGGTGA GCCTCTGGGA AAGCGCGGGG AGGCGAAGGT GACAGGCCTG 
CCACGGGGTA GCGCTGCCGC GCACTTCTGC GGGAGGCCAT TGGCGTCCTG GGGGCACCGG 
GAGGTTTCGG TTATCGGGAC CCCTTTTTGG GGGGTGCGGC TCAGCCCCCT AGCAAGAGAC 
AGGCCCTGCA GCCTAGCTGC TGGCAGTTGG GAAACCGAGG CAGGAAGCCG CAAGAAGAAG 
AGGAGGAAGA AGAAAGAGGC CGGCCCGCCC GGGCCTGCGC CCCAGCTCTG CGACGTGGGC 
TCCTTGGCCC CGCAGGCCCG CAGCGCCGTC CCACGCCTGC CCAAGCTGCC CAGGCAGAAG 
TGCTTGGTCT CCAGCCCCTG GGAGGCTCGG CTTTGGTCCC CGCCCCCCGC CGCCAGCCTG 
GCTCCTGGCG CCCTGCCCGG CGGGAGCGTC CCCTGCGGGT TGAGGACTCC AGGCCGAAGG 
GCAGGCTTCG CCCCCGTCCA TTCCCAGACG AACCTCATGG ATGCTGAGCT GCTGGATGCG 
GATTCGGACT TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025038991.1
CDS	1..3135
Translation	MSRPCSEHLGTFSICLTIIFLLQLAAGVLGFVFSDKARGKVSEIINNAIVHYRDDLDLQNLIDFGQKEFSCCGGVSYKDWSQNMYFNCTADNPSRERCSVPYSCCLHAADQPKYWTPPPHALRHSEKEKSGPPGEFQSFSTQDDNGANQSAFNLGVVFLCLLRGNLLTAVFPPRQFSICLTIIFLLQLAAGVLGFVFSDKARGKVSEIINNAIVHYRDDLDLQNLIDFGQKEFSCCGGVSYKDWSQNMYFNCTADNPSRERCSVPYSCCLHAADQAVINTMCGQGMQAMNYLEASEFIYPNGCIDKLLLWPGLRNAPRCWDAIQPLLCAVYMPKCEDGQVELPSQTLCQATRGPCTIVERERGWPDFLKCTTDRFPEGCPNEVQNIKFNSSGHCEAPLVQTDNPKSWYEDVEGCGIQCQNPLFTESEHRDMHVYIAAFSSVTIFCTFFTLATFVADWKNSNRYPAVILFYVNACFFVGSIGWLAQFMDGARGEIVCRADnullnullnullnullnullnullnullPSSNETLSCVIIFVIVYYSLMSGVIWFVMLTYAWHTSFKALGTTYQPLLGKTSYFHLVTWAIPFVLTVAILAVAQVDGDSVSGICFVGYKNYRYRAGFVLAPIGLVLIVGGYFLIRGVMTLFSIKSNHPGLLSEKAASKINETMLRLGKRGPGGPGLWGTAGVHGKLCGAREVGAAGVHEDPRGVEGARAMLFIHTNKPIPNCEIKNRPSLLVEKINLFAMFGTGISMSTWVWTKATLLIWKRTWCRLTGQSDDEPKRIKKSKMIAKAFSRRKELLHNPEQELSPLNESRFVQRRQQFLLAVPPLRWEPDSRPAPGLAFDLNEPSADVSSAWAQHVTKMVARRGAILPQDISVTPVATPGEPLGKRGEAKVTGLPRGSAAAHFCGRPLASWGHREVSVIGTPFWGVRLSPLARDRPCSLAAGSWETEAGSRKKKRRKKKEAGPPGPAPQLCDVGSLAPQARSAVPRLPKLPRQKCLVSSPWEARLWSPPPAASLAPGALPGGSVPCGLRTPGRRAGFAPVHSQTNLMDAELLDADSDF

Target ORF information:

RefSeq Version	XM_025183206.1
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis smoothened, frizzled class receptor (SMO), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025183206.1

ORF Insert Sequence:

ATGTCACGCC CGTGCTCTGA GCACTTGGGG ACGTTCTCAA TCTGCTTGAC CATCATCTTC 
CTGCTGCAGC TGGCAGCTGG AGTACTGGGC TTTGTCTTCT CGGATAAGGC ACGCGGGAAG 
GTGAGCGAGA TCATCAACAA CGCCATCGTG CATTACCGGG ACGACCTGGA CCTGCAGAAC 
CTCATCGACT TCGGCCAGAA GGAGTTCAGT TGCTGTGGGG GTGTCTCCTA CAAGGACTGG 
TCCCAAAACA TGTATTTCAA CTGCACTGCT GACAACCCCA GCCGGGAGCG CTGTTCCGTG 
CCTTATTCTT GCTGCCTGCA CGCTGCCGAC CAGCCAAAAT ATTGGACTCC TCCGCCACAT 
GCCCTGCGCC ATTCAGAAAA AGAGAAGTCA GGACCACCTG GGGAATTCCA GAGCTTCAGT 
ACCCAGGACG ATAATGGAGC AAACCAATCA GCCTTCAATT TGGGTGTTGT CTTTCTTTGC 
CTCCTCCGTG GCAATTTGCT GACCGCTGTT TTCCCTCCCC GGCAGTTCTC AATCTGCTTG 
ACCATCATCT TCCTGCTGCA GCTGGCAGCT GGAGTACTGG GCTTTGTCTT CTCGGATAAG 
GCACGCGGGA AGGTGAGCGA GATCATCAAC AACGCCATCG TGCATTACCG GGACGACCTG 
GACCTGCAGA ACCTCATCGA CTTCGGCCAG AAGGAGTTCA GTTGCTGTGG GGGTGTCTCC 
TACAAGGACT GGTCCCAAAA CATGTATTTC AACTGCACTG CTGACAACCC CAGCCGGGAG 
CGCTGTTCCG TGCCTTATTC TTGCTGCCTG CACGCTGCCG ACCAGGCCGT CATCAACACC 
ATGTGTGGCC AGGGCATGCA AGCCATGAAC TACCTGGAGG CCAGCGAGTT CATCTACCCC 
AACGGCTGCA TCGACAAGCT GCTGCTCTGG CCAGGCTTGC GGAACGCCCC GCGCTGCTGG 
GACGCCATCC AGCCGCTGCT CTGCGCCGTG TACATGCCCA AGTGCGAGGA CGGCCAGGTG 
GAGCTGCCCA GCCAGACCTT GTGCCAGGCC ACCCGGGGCC CGTGCACCAT CGTGGAACGG 
GAGCGGGGCT GGCCCGACTT CCTCAAGTGC ACCACGGACC GCTTTCCGGA AGGCTGCCCG 
AACGAGGTGC AGAACATCAA ATTCAACAGC TCGGGGCACT GCGAGGCGCC CCTGGTGCAG 
ACGGACAACC CCAAGAGCTG GTACGAGGAC GTGGAGGGCT GCGGCATCCA GTGCCAGAAC 
CCCCTCTTCA CGGAGAGCGA GCACCGGGAC ATGCACGTGT ACATCGCCGC CTTCAGCTCC 
GTCACCATCT TCTGCACCTT CTTCACCCTG GCCACGTTCG TTGCCGACTG GAAGAACTCG 
AACCGTTACC CGGCCGTCAT CCTCTTCTAC GTCAACGCCT GCTTCTTTGT GGGCAGCATT 
GGCTGGCTGG CGCAGTTCAT GGACGGGGCC CGCGGCGAGA TCGTGTGCCG AGCCGACNNN 
NNNNNNNNNN NNNNNNTCCC CAGCTCCAAC GAGACGCTCT CCTGCGTGAT CATCTTCGTC 
ATCGTCTACT ACTCGCTGAT GTCGGGCGTG ATCTGGTTCG TCATGCTGAC CTACGCCTGG 
CACACCTCGT TCAAGGCCCT GGGCACCACC TACCAGCCGC TGCTGGGCAA GACCTCCTAC 
TTCCACCTGG TCACCTGGGC CATCCCTTTC GTCCTCACCG TCGCCATCCT GGCTGTCGCC 
CAGGTGGATG GAGACTCCGT CAGCGGCATC TGCTTTGTGG GCTACAAGAA CTATCGCTAC 
CGGGCCGGCT TCGTCCTGGC GCCCATCGGC CTGGTGCTGA TCGTGGGAGG CTACTTCCTC 
ATCCGAGGCG TCATGACGTT GTTCTCCATC AAAAGTAACC ACCCCGGCCT GCTGAGCGAG 
AAGGCGGCCA GCAAAATCAA CGAGACCATG TTACGGCTGG GTAAGCGGGG CCCGGGCGGG 
CCTGGCTTGT GGGGGACCGC TGGTGTCCAT GGGAAGCTCT GTGGTGCGCG AGAAGTGGGT 
GCCGCTGGTG TTCACGAGGA CCCTAGAGGT GTCGAAGGAG CCCGAGCTAT GCTTTTCATC 
CACACCAACA AGCCCATCCC CAACTGCGAG ATCAAGAACC GGCCCAGCCT GCTGGTGGAG 
AAGATCAACC TCTTCGCCAT GTTCGGCACC GGCATCTCCA TGAGCACCTG GGTGTGGACC 
AAAGCCACCC TCCTCATCTG GAAGCGCACC TGGTGCAGGC TGACGGGACA GAGCGATGAC 
GAGCCCAAGA GGATCAAGAA AAGCAAGATG ATTGCCAAGG CCTTCTCCCG GCGGAAGGAG 
CTGCTGCACA ACCCGGAGCA GGAGCTGTCC CCTCTTAATG AGAGCCGCTT TGTCCAGCGA 
CGACAACAGT TTTTACTCGC TGTTCCTCCT CTCCGCTGGG AGCCAGATTC AAGGCCGGCT 
CCTGGTTTGG CTTTCGACCT CAACGAGCCG TCAGCCGACG TGTCCTCGGC CTGGGCCCAG 
CACGTCACCA AGATGGTGGC CAGGAGAGGA GCCATCCTGC CTCAGGACAT CTCGGTCACA 
CCCGTGGCGA CGCCTGGTGA GCCTCTGGGA AAGCGCGGGG AGGCGAAGGT GACAGGCCTG 
CCACGGGGTA GCGCTGCCGC GCACTTCTGC GGGAGGCCAT TGGCGTCCTG GGGGCACCGG 
GAGGTTTCGG TTATCGGGAC CCCTTTTTGG GGGGTGCGGC TCAGCCCCCT AGCAAGAGAC 
AGGCCCTGCA GCCTAGCTGC TGGCAGTTGG GAAACCGAGG CAGGAAGCCG CAAGAAGAAG 
AGGAGGAAGA AGAAAGAGGC CGGCCCGCCC GGGCCTGCGC CCCAGCTCTG CGACGTGGGC 
TCCTTGGCCC CGCAGGCCCG CAGCGCCGTC CCACGCCTGC CCAAGCTGCC CAGGCAGAAG 
TGCTTGGTCT CCAGCCCCTG GGAGGCTCGG CTTTGGTCCC CGCCCCCCGC CGCCAGCCTG 
GCTCCTGGCG CCCTGCCCGG CGGGAGCGTC CCCTGCGGGT TGAGGACTCC AGGCCGAAGG 
GCAGGCTTCG CCCCCGTCCA TTCCCAGACG AACCTCATGG ATGCTGAGCT GCTGGATGCG 
GATTCGGACT TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMO ( XM_014572119.1 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_014427605.1 Pelodiscus sinensis smoothened, frizzled class receptor (SMO), mRNA.

Price & Availability↑

CloneID	OPe26765
Clone ID Related Accession (Same CDS sequence)	XM_014572119.1
Accession Version	XM_014572119.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-02
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	smoothened homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005854397.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pelodiscus sinensis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 13% of CDS bases ##RefSeq-Attributes-END##

ATGGAGCACG TCCTAGCCTG GTTCCATTAC ATGCCCCGTC CTTGGCACAA GGACTGTCGT 
GGTCATCGGG GATTGACCCG GCAGCGTCCG CATCAGAAAC GCAGGCCTTG CCTGGCCGAG 
CTAAGGGAGT GTCCTTCAGT GGGACTGAGA GGACAGCGTG CGACTCCCGT GCCGAATTCC 
CACGGCGGCT TTCTCCCTCG GCCAGGCTTG CGGAACGCCC CGCGCTGCTG GGACGCCATC 
CAGCCGCTGC TCTGCGCCGT GTACATGCCC AAGTGCGAGG ACGGCCAGGT GGAGCTGCCC 
AGCCAGACCT TGTGCCAGGC CACCCGGGGC CCGTGCACCA TCGTGGAACG GGAGCGGGGC 
TGGCCCGACT TCCTCAAGTG CACCACGGAC CGCTTTCCGG AAGGCTGCCC GAACGAGGTG 
CAGAACATCA AATTCAACAG CTCGGGGCAC TGCGAGGCGC CCCTGGTGCA GACGGACAAC 
CCCAAGAGCT GGTACGAGGA CGTGGAGGGC TGCGGCATCC AGTGCCAGAA CCCCCTCTTC 
ACGGAGAGCG AGCACCGGGA CATGCACGTG TACATCGCCG CCTTCAGCTC CGTCACCATC 
TTCTGCACCT TCTTCACCCT GGCCACGTTC GTTGCCGACT GGAAGAACTC GAACCGTTAC 
CCGGCCGTCA TCCTCTTCTA CGTCAACGCC TGCTTCTTTG TGGGCAGCAT TGGCTGGCTG 
GCGCAGTTCA TGGACGGGGC CCGCGGCGAG ATCGTGTGCC GAGCCGACNN NNNNNNNNNN 
NNNNNNNTCC CCAGCTCCAA CGAGACGCTC TCCTGCGTGA TCATCTTCGT CATCGTCTAC 
TACTCGCTGA TGTCGGGCGT GATCTGGTTC GTCATGCTGA CCTACGCCTG GCACACCTCG 
TTCAAGGCCC TGGGCACCAC CTACCAGCCG CTGCTGGGCA AGACCTCCTA CTTCCACCTG 
GTCACCTGGG CCATCCCTTT CGTCCTCACC GTCGCCATCC TGGCTGTCGC CCAGGTGGAT 
GGAGACTCCG TCAGCGGCAT CTGCTTTGTG GGCTACAAGA ACTATCGCTA CCGGGCCGGC 
TTCGTCCTGG CGCCCATCGG CCTGGTGCTG ATCGTGGGAG GCTACTTCCT CATCCGAGGC 
GTCATGACGT TGTTCTCCAT CAAAAGTAAC CACCCCGGCC TGCTGAGCGA GAAGGCGGCC 
AGCAAAATCA ACGAGACCAT GTTACGGCTG GGTAAGCGGG GCCCGGGCGG GCCTGGCTTG 
TGGGGGACCG CTGGTGTCCA TGGGAAGCTC TGTGGTGCGC GAGAAGTGGG TGCCGCTGGT 
GTTCACGAGG ACCCTAGAGT CATCCACACC AACAAGCCCA TCCCCAACTG CGAGATCAAG 
AACCGGCCCA GCCTGCTGGT GGAGAAGATC AACCTCTTCG CCATGTTCGG CACCGGCATC 
TCCATGAGCA CCTGGGTGTG GACCAAAGCC ACCCTCCTCA TCTGGAAGCG CACCTGGTGC 
AGGCTGACGG GACAGAGCGA TGACGAGCCC AAGAGGATCA AGAAAAGCAA GATGATTGCC 
AAGGCCTTCT CCCGGCGGAA GGAGCTGCTG CACAACCCGG AGCAGGAGCT AATGAGAGCC 
GCTTTGTCCA GCGACGACAA CAGTTTTTAC TCGCTGTTCC TCCTCTCCGC TGGGAGCCAG 
ATTCAAGCTG GTTTGGCTTT CGACCTCAAC GAGCCGTCAG CCGACGTGTC CTCGGCCTGG 
GCCCAGCACG TCACCAAGAT GGTGGCCAGG AGAGGAGCCA TCCTGCCTCA GGACATCTCG 
GTCACACCCG TGGCGACGCC TGGTGAGCCT CTGGGAAAGC GCGGGGAGGC GAAGGTGACA 
GGCCTGCCAC GGGGCCGCAA GAAGAAGAGG AGGAAGAAGA AAGAGGCCGG CCCGCCCGGG 
CCTGCGCCCC AGCTCTGCGA CGTGGGCTCC TTGGCCCCGC AGGCCCGCAG CGCCGTCCCA 
CGCCTGCCCA AGCTGCCCAG GCAGAAGTGC TTGGTCTCCA GCCCCTGGGA GGCTCGGCTT 
TGGTCCCCGC CCCCCGCCGC CAGCCTGGCT CCTGGCGCCC TGCCCGGCGG GAGCGTCCCC 
TGCGGGTTGA GGACTCCAGG CCGAAGGGCA GGCTTCGCCC CCGTCCATTC CCAGACGAAC 
CTCATGGATG CTGAGCTGCT GGATGCGGAT TCGGACTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014427605.1
CDS	1..2202
Translation	MEHVLAWFHYMPRPWHKDCRGHRGLTRQRPHQKRRPCLAELRECPSVGLRGQRATPVPNSHGGFLPRPGLRNAPRCWDAIQPLLCAVYMPKCEDGQVELPSQTLCQATRGPCTIVERERGWPDFLKCTTDRFPEGCPNEVQNIKFNSSGHCEAPLVQTDNPKSWYEDVEGCGIQCQNPLFTESEHRDMHVYIAAFSSVTIFCTFFTLATFVADWKNSNRYPAVILFYVNACFFVGSIGWLAQFMDGARGEIVCRADnullnullnullnullnullnullnullPSSNETLSCVIIFVIVYYSLMSGVIWFVMLTYAWHTSFKALGTTYQPLLGKTSYFHLVTWAIPFVLTVAILAVAQVDGDSVSGICFVGYKNYRYRAGFVLAPIGLVLIVGGYFLIRGVMTLFSIKSNHPGLLSEKAASKINETMLRLGKRGPGGPGLWGTAGVHGKLCGAREVGAAGVHEDPRVIHTNKPIPNCEIKNRPSLLVEKINLFAMFGTGISMSTWVWTKATLLIWKRTWCRLTGQSDDEPKRIKKSKMIAKAFSRRKELLHNPEQELMRAALSSDDNSFYSLFLLSAGSQIQAGLAFDLNEPSADVSSAWAQHVTKMVARRGAILPQDISVTPVATPGEPLGKRGEAKVTGLPRGRKKKRRKKKEAGPPGPAPQLCDVGSLAPQARSAVPRLPKLPRQKCLVSSPWEARLWSPPPAASLAPGALPGGSVPCGLRTPGRRAGFAPVHSQTNLMDAELLDADSDF

Target ORF information:

RefSeq Version	XM_014572119.1
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis smoothened, frizzled class receptor (SMO), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014572119.1

ORF Insert Sequence:

ATGGAGCACG TCCTAGCCTG GTTCCATTAC ATGCCCCGTC CTTGGCACAA GGACTGTCGT 
GGTCATCGGG GATTGACCCG GCAGCGTCCG CATCAGAAAC GCAGGCCTTG CCTGGCCGAG 
CTAAGGGAGT GTCCTTCAGT GGGACTGAGA GGACAGCGTG CGACTCCCGT GCCGAATTCC 
CACGGCGGCT TTCTCCCTCG GCCAGGCTTG CGGAACGCCC CGCGCTGCTG GGACGCCATC 
CAGCCGCTGC TCTGCGCCGT GTACATGCCC AAGTGCGAGG ACGGCCAGGT GGAGCTGCCC 
AGCCAGACCT TGTGCCAGGC CACCCGGGGC CCGTGCACCA TCGTGGAACG GGAGCGGGGC 
TGGCCCGACT TCCTCAAGTG CACCACGGAC CGCTTTCCGG AAGGCTGCCC GAACGAGGTG 
CAGAACATCA AATTCAACAG CTCGGGGCAC TGCGAGGCGC CCCTGGTGCA GACGGACAAC 
CCCAAGAGCT GGTACGAGGA CGTGGAGGGC TGCGGCATCC AGTGCCAGAA CCCCCTCTTC 
ACGGAGAGCG AGCACCGGGA CATGCACGTG TACATCGCCG CCTTCAGCTC CGTCACCATC 
TTCTGCACCT TCTTCACCCT GGCCACGTTC GTTGCCGACT GGAAGAACTC GAACCGTTAC 
CCGGCCGTCA TCCTCTTCTA CGTCAACGCC TGCTTCTTTG TGGGCAGCAT TGGCTGGCTG 
GCGCAGTTCA TGGACGGGGC CCGCGGCGAG ATCGTGTGCC GAGCCGACNN NNNNNNNNNN 
NNNNNNNTCC CCAGCTCCAA CGAGACGCTC TCCTGCGTGA TCATCTTCGT CATCGTCTAC 
TACTCGCTGA TGTCGGGCGT GATCTGGTTC GTCATGCTGA CCTACGCCTG GCACACCTCG 
TTCAAGGCCC TGGGCACCAC CTACCAGCCG CTGCTGGGCA AGACCTCCTA CTTCCACCTG 
GTCACCTGGG CCATCCCTTT CGTCCTCACC GTCGCCATCC TGGCTGTCGC CCAGGTGGAT 
GGAGACTCCG TCAGCGGCAT CTGCTTTGTG GGCTACAAGA ACTATCGCTA CCGGGCCGGC 
TTCGTCCTGG CGCCCATCGG CCTGGTGCTG ATCGTGGGAG GCTACTTCCT CATCCGAGGC 
GTCATGACGT TGTTCTCCAT CAAAAGTAAC CACCCCGGCC TGCTGAGCGA GAAGGCGGCC 
AGCAAAATCA ACGAGACCAT GTTACGGCTG GGTAAGCGGG GCCCGGGCGG GCCTGGCTTG 
TGGGGGACCG CTGGTGTCCA TGGGAAGCTC TGTGGTGCGC GAGAAGTGGG TGCCGCTGGT 
GTTCACGAGG ACCCTAGAGT CATCCACACC AACAAGCCCA TCCCCAACTG CGAGATCAAG 
AACCGGCCCA GCCTGCTGGT GGAGAAGATC AACCTCTTCG CCATGTTCGG CACCGGCATC 
TCCATGAGCA CCTGGGTGTG GACCAAAGCC ACCCTCCTCA TCTGGAAGCG CACCTGGTGC 
AGGCTGACGG GACAGAGCGA TGACGAGCCC AAGAGGATCA AGAAAAGCAA GATGATTGCC 
AAGGCCTTCT CCCGGCGGAA GGAGCTGCTG CACAACCCGG AGCAGGAGCT AATGAGAGCC 
GCTTTGTCCA GCGACGACAA CAGTTTTTAC TCGCTGTTCC TCCTCTCCGC TGGGAGCCAG 
ATTCAAGCTG GTTTGGCTTT CGACCTCAAC GAGCCGTCAG CCGACGTGTC CTCGGCCTGG 
GCCCAGCACG TCACCAAGAT GGTGGCCAGG AGAGGAGCCA TCCTGCCTCA GGACATCTCG 
GTCACACCCG TGGCGACGCC TGGTGAGCCT CTGGGAAAGC GCGGGGAGGC GAAGGTGACA 
GGCCTGCCAC GGGGCCGCAA GAAGAAGAGG AGGAAGAAGA AAGAGGCCGG CCCGCCCGGG 
CCTGCGCCCC AGCTCTGCGA CGTGGGCTCC TTGGCCCCGC AGGCCCGCAG CGCCGTCCCA 
CGCCTGCCCA AGCTGCCCAG GCAGAAGTGC TTGGTCTCCA GCCCCTGGGA GGCTCGGCTT 
TGGTCCCCGC CCCCCGCCGC CAGCCTGGCT CCTGGCGCCC TGCCCGGCGG GAGCGTCCCC 
TGCGGGTTGA GGACTCCAGG CCGAAGGGCA GGCTTCGCCC CCGTCCATTC CCAGACGAAC 
CTCATGGATG CTGAGCTGCT GGATGCGGAT TCGGACTTCT AG