MID1 cDNA ORF clone, Pan troglodytes(chimpanzee)

The following MID1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MID1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OPc110268 XM_024353273.1
Latest version!
Pan troglodytes midline 1 (MID1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OPc110268 XM_024353272.1
Latest version!
Pan troglodytes midline 1 (MID1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OPc110269 XM_024353274.1
Latest version!
Pan troglodytes midline 1 (MID1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OPc90180 XM_016942744.1
Latest version!
Pan troglodytes midline 1 (MID1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OPc110268
    Clone ID Related Accession (Same CDS sequence) XM_024353272.1 , XM_024353273.1
    Accession Version XM_024353272.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1137bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036902.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAGGCTTC GCAAACTGGC TCAGCAGATT GCAAACTGCA AACAGTGCAT TGAGCGGTCA 
    GCATCACTCA TCTCCCAAGC GGAACACTCT CTGAAGGAGA ATGATCATGC GCGTTTCCTA
    CAGACTGCTA AGAATATCAC CGAGAGAGTC TCCATGGCAA CTGCATCCTC CCAGGTTCTA
    ATTCCTGAAA TCAACCTCAA TGACACATTT GACACCTTTG CCTTAGATTT TTCCCGAGAG
    AAGAAATTGC TAGAATGTCT GGATTACCTT ACAGCTCCCA ACCCTCCCAC AATTAGAGAA
    GAGCTCTGCA CAGCTTCATA TGACACCATC ACTGTGCATT GGACCTCCGA TGATGAGTTC
    AGTGTGGTCT CCTACGAGCT CCAGTACACC ATATTCACCG GACAAGCCAA CGTCGTTAGT
    CTGTGTAATT CGGCTGATAG CTGGATGATA GTACCCAACA TCAAGCAGAA CCACTACACG
    GTGCACGGTC TGCAGAGCGG CACCAAGTAC ATCTTCATGG TCAAGGCCAT CAACCAGGCG
    GGCAGCCGCA GCAGTGAGCC TGGGAAGTTG AAGACAAACA GCCAACCATT TAAACTGGAT
    CCCAAATCTG CTCATCGAAA ACTGAAGGTG TCCCATGATA ACTTGACAGT AGAACGTGAT
    GAGTCATCAT CCAAGAAGAG TCACACACCT GAACGCTTCA CCAGCCAGGG GAGCTATGGA
    GTAGCTGGAA ATGTGTTTAT TGATAGTGGC CGGCATTATT GGGAAGTGGT CATAAGTGGA
    AGCACATGGT ATGCCATTGG TCTTGCTTAC AAATCAGCCC CGAAGCATGA ATGGATTGGG
    AAGAACTCTG CTTCCTGGGC GCTCTGCCGC TGCAACAATA ACTGGGTGGT GAGACACAAT
    AGCAAGGAAA TCCCCATTGA GCCTGCCCCC CACCTCCGGC GCGTGGGCAT CTTGCTGGAC
    TATGATAACG GCTCTATCGC CTTTTATGAT GCTTTGAACT CCATCCACCT CTACACCTTC
    GACGTCGCAT TTGCGCAGCC TGTTTGCCCC ACCTTCACCG TGTGGAACAA GTGTCTGACG
    ATTATCACTG GGCTCCCTAT CCCAGACCAT TTGGACTGCA CAGAGCAGCT GCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024209040.1
    CDS899..2035
    Translation

    Target ORF information:

    RefSeq Version XM_024353272.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes midline 1 (MID1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024353272.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAGGCTTC GCAAACTGGC TCAGCAGATT GCAAACTGCA AACAGTGCAT TGAGCGGTCA 
    GCATCACTCA TCTCCCAAGC GGAACACTCT CTGAAGGAGA ATGATCATGC GCGTTTCCTA
    CAGACTGCTA AGAATATCAC CGAGAGAGTC TCCATGGCAA CTGCATCCTC CCAGGTTCTA
    ATTCCTGAAA TCAACCTCAA TGACACATTT GACACCTTTG CCTTAGATTT TTCCCGAGAG
    AAGAAATTGC TAGAATGTCT GGATTACCTT ACAGCTCCCA ACCCTCCCAC AATTAGAGAA
    GAGCTCTGCA CAGCTTCATA TGACACCATC ACTGTGCATT GGACCTCCGA TGATGAGTTC
    AGTGTGGTCT CCTACGAGCT CCAGTACACC ATATTCACCG GACAAGCCAA CGTCGTTAGT
    CTGTGTAATT CGGCTGATAG CTGGATGATA GTACCCAACA TCAAGCAGAA CCACTACACG
    GTGCACGGTC TGCAGAGCGG CACCAAGTAC ATCTTCATGG TCAAGGCCAT CAACCAGGCG
    GGCAGCCGCA GCAGTGAGCC TGGGAAGTTG AAGACAAACA GCCAACCATT TAAACTGGAT
    CCCAAATCTG CTCATCGAAA ACTGAAGGTG TCCCATGATA ACTTGACAGT AGAACGTGAT
    GAGTCATCAT CCAAGAAGAG TCACACACCT GAACGCTTCA CCAGCCAGGG GAGCTATGGA
    GTAGCTGGAA ATGTGTTTAT TGATAGTGGC CGGCATTATT GGGAAGTGGT CATAAGTGGA
    AGCACATGGT ATGCCATTGG TCTTGCTTAC AAATCAGCCC CGAAGCATGA ATGGATTGGG
    AAGAACTCTG CTTCCTGGGC GCTCTGCCGC TGCAACAATA ACTGGGTGGT GAGACACAAT
    AGCAAGGAAA TCCCCATTGA GCCTGCCCCC CACCTCCGGC GCGTGGGCAT CTTGCTGGAC
    TATGATAACG GCTCTATCGC CTTTTATGAT GCTTTGAACT CCATCCACCT CTACACCTTC
    GACGTCGCAT TTGCGCAGCC TGTTTGCCCC ACCTTCACCG TGTGGAACAA GTGTCTGACG
    ATTATCACTG GGCTCCCTAT CCCAGACCAT TTGGACTGCA CAGAGCAGCT GCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc110268
    Clone ID Related Accession (Same CDS sequence) XM_024353272.1 , XM_024353273.1
    Accession Version XM_024353273.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1137bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036902.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAGGCTTC GCAAACTGGC TCAGCAGATT GCAAACTGCA AACAGTGCAT TGAGCGGTCA 
    GCATCACTCA TCTCCCAAGC GGAACACTCT CTGAAGGAGA ATGATCATGC GCGTTTCCTA
    CAGACTGCTA AGAATATCAC CGAGAGAGTC TCCATGGCAA CTGCATCCTC CCAGGTTCTA
    ATTCCTGAAA TCAACCTCAA TGACACATTT GACACCTTTG CCTTAGATTT TTCCCGAGAG
    AAGAAATTGC TAGAATGTCT GGATTACCTT ACAGCTCCCA ACCCTCCCAC AATTAGAGAA
    GAGCTCTGCA CAGCTTCATA TGACACCATC ACTGTGCATT GGACCTCCGA TGATGAGTTC
    AGTGTGGTCT CCTACGAGCT CCAGTACACC ATATTCACCG GACAAGCCAA CGTCGTTAGT
    CTGTGTAATT CGGCTGATAG CTGGATGATA GTACCCAACA TCAAGCAGAA CCACTACACG
    GTGCACGGTC TGCAGAGCGG CACCAAGTAC ATCTTCATGG TCAAGGCCAT CAACCAGGCG
    GGCAGCCGCA GCAGTGAGCC TGGGAAGTTG AAGACAAACA GCCAACCATT TAAACTGGAT
    CCCAAATCTG CTCATCGAAA ACTGAAGGTG TCCCATGATA ACTTGACAGT AGAACGTGAT
    GAGTCATCAT CCAAGAAGAG TCACACACCT GAACGCTTCA CCAGCCAGGG GAGCTATGGA
    GTAGCTGGAA ATGTGTTTAT TGATAGTGGC CGGCATTATT GGGAAGTGGT CATAAGTGGA
    AGCACATGGT ATGCCATTGG TCTTGCTTAC AAATCAGCCC CGAAGCATGA ATGGATTGGG
    AAGAACTCTG CTTCCTGGGC GCTCTGCCGC TGCAACAATA ACTGGGTGGT GAGACACAAT
    AGCAAGGAAA TCCCCATTGA GCCTGCCCCC CACCTCCGGC GCGTGGGCAT CTTGCTGGAC
    TATGATAACG GCTCTATCGC CTTTTATGAT GCTTTGAACT CCATCCACCT CTACACCTTC
    GACGTCGCAT TTGCGCAGCC TGTTTGCCCC ACCTTCACCG TGTGGAACAA GTGTCTGACG
    ATTATCACTG GGCTCCCTAT CCCAGACCAT TTGGACTGCA CAGAGCAGCT GCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024209041.1
    CDS1118..2254
    Translation

    Target ORF information:

    RefSeq Version XM_024353273.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes midline 1 (MID1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024353273.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAGGCTTC GCAAACTGGC TCAGCAGATT GCAAACTGCA AACAGTGCAT TGAGCGGTCA 
    GCATCACTCA TCTCCCAAGC GGAACACTCT CTGAAGGAGA ATGATCATGC GCGTTTCCTA
    CAGACTGCTA AGAATATCAC CGAGAGAGTC TCCATGGCAA CTGCATCCTC CCAGGTTCTA
    ATTCCTGAAA TCAACCTCAA TGACACATTT GACACCTTTG CCTTAGATTT TTCCCGAGAG
    AAGAAATTGC TAGAATGTCT GGATTACCTT ACAGCTCCCA ACCCTCCCAC AATTAGAGAA
    GAGCTCTGCA CAGCTTCATA TGACACCATC ACTGTGCATT GGACCTCCGA TGATGAGTTC
    AGTGTGGTCT CCTACGAGCT CCAGTACACC ATATTCACCG GACAAGCCAA CGTCGTTAGT
    CTGTGTAATT CGGCTGATAG CTGGATGATA GTACCCAACA TCAAGCAGAA CCACTACACG
    GTGCACGGTC TGCAGAGCGG CACCAAGTAC ATCTTCATGG TCAAGGCCAT CAACCAGGCG
    GGCAGCCGCA GCAGTGAGCC TGGGAAGTTG AAGACAAACA GCCAACCATT TAAACTGGAT
    CCCAAATCTG CTCATCGAAA ACTGAAGGTG TCCCATGATA ACTTGACAGT AGAACGTGAT
    GAGTCATCAT CCAAGAAGAG TCACACACCT GAACGCTTCA CCAGCCAGGG GAGCTATGGA
    GTAGCTGGAA ATGTGTTTAT TGATAGTGGC CGGCATTATT GGGAAGTGGT CATAAGTGGA
    AGCACATGGT ATGCCATTGG TCTTGCTTAC AAATCAGCCC CGAAGCATGA ATGGATTGGG
    AAGAACTCTG CTTCCTGGGC GCTCTGCCGC TGCAACAATA ACTGGGTGGT GAGACACAAT
    AGCAAGGAAA TCCCCATTGA GCCTGCCCCC CACCTCCGGC GCGTGGGCAT CTTGCTGGAC
    TATGATAACG GCTCTATCGC CTTTTATGAT GCTTTGAACT CCATCCACCT CTACACCTTC
    GACGTCGCAT TTGCGCAGCC TGTTTGCCCC ACCTTCACCG TGTGGAACAA GTGTCTGACG
    ATTATCACTG GGCTCCCTAT CCCAGACCAT TTGGACTGCA CAGAGCAGCT GCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc110269
    Clone ID Related Accession (Same CDS sequence) XM_024353274.1
    Accession Version XM_024353274.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 975bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product E3 ubiquitin-protein ligase Midline-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036902.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAGGCTTC GCAAACTGGC TCAGCAGATT GCAAACTGCA AACAGTGCAT TGAGCGGTCA 
    GCATCACTCA TCTCCCAAGC GGAACACTCT CTGAAGGAGA ATGATCATGC GCGTTTCCTA
    CAGACTGCTA AGAATATCAC CGAGAGAGTC TCCATGGCAA CTGCATCCTC CCAGGTTCTA
    ATTCCTGAAA TCAACCTCAA TGACACATTT GACACCTTTG CCTTAGATTT TTCCCGAGAG
    AAGAAATTGC TAGAATGTCT GGATTACCTT ACAGCTCCCA ACCCTCCCAC AATTAGAGAA
    GAGCTCTGCA CAGCTTCATA TGACACCATC ACTGTGCATT GGACCTCCGA TGATGAGTTC
    AGTGTGGTCT CCTACGAGCT CCAGTACACC ATATTCACCG GACAAGCCAA CGTCGTTAGC
    CAACCATTTA AACTGGATCC CAAATCTGCT CATCGAAAAC TGAAGGTGTC CCATGATAAC
    TTGACAGTAG AACGTGATGA GTCATCATCC AAGAAGAGTC ACACACCTGA ACGCTTCACC
    AGCCAGGGGA GCTATGGAGT AGCTGGAAAT GTGTTTATTG ATAGTGGCCG GCATTATTGG
    GAAGTGGTCA TAAGTGGAAG CACATGGTAT GCCATTGGTC TTGCTTACAA ATCAGCCCCG
    AAGCATGAAT GGATTGGGAA GAACTCTGCT TCCTGGGCGC TCTGCCGCTG CAACAATAAC
    TGGGTGGTGA GACACAATAG CAAGGAAATC CCCATTGAGC CTGCCCCCCA CCTCCGGCGC
    GTGGGCATCT TGCTGGACTA TGATAACGGC TCTATCGCCT TTTATGATGC TTTGAACTCC
    ATCCACCTCT ACACCTTCGA CGTCGCATTT GCGCAGCCTG TTTGCCCCAC CTTCACCGTG
    TGGAACAAGT GTCTGACGAT TATCACTGGG CTCCCTATCC CAGACCATTT GGACTGCACA
    GAGCAGCTGC CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024209042.1
    CDS1388..2362
    Translation

    Target ORF information:

    RefSeq Version XM_024353274.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes midline 1 (MID1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024353274.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAGGCTTC GCAAACTGGC TCAGCAGATT GCAAACTGCA AACAGTGCAT TGAGCGGTCA 
    GCATCACTCA TCTCCCAAGC GGAACACTCT CTGAAGGAGA ATGATCATGC GCGTTTCCTA
    CAGACTGCTA AGAATATCAC CGAGAGAGTC TCCATGGCAA CTGCATCCTC CCAGGTTCTA
    ATTCCTGAAA TCAACCTCAA TGACACATTT GACACCTTTG CCTTAGATTT TTCCCGAGAG
    AAGAAATTGC TAGAATGTCT GGATTACCTT ACAGCTCCCA ACCCTCCCAC AATTAGAGAA
    GAGCTCTGCA CAGCTTCATA TGACACCATC ACTGTGCATT GGACCTCCGA TGATGAGTTC
    AGTGTGGTCT CCTACGAGCT CCAGTACACC ATATTCACCG GACAAGCCAA CGTCGTTAGC
    CAACCATTTA AACTGGATCC CAAATCTGCT CATCGAAAAC TGAAGGTGTC CCATGATAAC
    TTGACAGTAG AACGTGATGA GTCATCATCC AAGAAGAGTC ACACACCTGA ACGCTTCACC
    AGCCAGGGGA GCTATGGAGT AGCTGGAAAT GTGTTTATTG ATAGTGGCCG GCATTATTGG
    GAAGTGGTCA TAAGTGGAAG CACATGGTAT GCCATTGGTC TTGCTTACAA ATCAGCCCCG
    AAGCATGAAT GGATTGGGAA GAACTCTGCT TCCTGGGCGC TCTGCCGCTG CAACAATAAC
    TGGGTGGTGA GACACAATAG CAAGGAAATC CCCATTGAGC CTGCCCCCCA CCTCCGGCGC
    GTGGGCATCT TGCTGGACTA TGATAACGGC TCTATCGCCT TTTATGATGC TTTGAACTCC
    ATCCACCTCT ACACCTTCGA CGTCGCATTT GCGCAGCCTG TTTGCCCCAC CTTCACCGTG
    TGGAACAAGT GTCTGACGAT TATCACTGGG CTCCCTATCC CAGACCATTT GGACTGCACA
    GAGCAGCTGC CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc90180
    Clone ID Related Accession (Same CDS sequence) XM_016942744.1
    Accession Version XM_016942744.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2010bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product E3 ubiquitin-protein ligase Midline-1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006491.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
    CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA
    CACTGTGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG
    CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG
    AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC
    CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG
    TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC
    TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT
    CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG
    ACTGGACTCA AAGGGAACCT GACATCTTCT CTGGAATTGA AGCCAAACAC AGAACTCTCA
    TGGAAGGAAG CAGAGGCACT GAGGATTTGC ATTTCTCTGG AAGTCAATGC ATCACGTCAA
    GAAGCCAAAT TGACAGAGGA GTGTGATCTT CTCATTGAGA TCATTCAGCA AAGACGACAG
    ATTATTGGAA CCAAGATCAA AGAAGGGAAG GTGATGAGGC TTCGCAAACT GGCTCAGCAG
    ATTGCAAACT GCAAACAGTG CATTGAGCGG TCAGCATCAC TCATCTCCCA AGCGGAACAC
    TCTCTGAAGG AGAATGATCA TGCGCGTTTC CTACAGACTG CTAAGAATAT CACCGAGAGA
    GTCTCCATGG CAACTGCATC CTCCCAGGTT CTAATTCCTG AAATCAACCT CAATGACACA
    TTTGACACCT TTGCCTTAGA TTTTTCCCGA GAGAAGAAAT TGCTAGAATG TCTGGATTAC
    CTTACAGCTC CCAACCCTCC CACAATTAGA GAAGAGCTCT GCACAGCTTC ATATGACACC
    ATCACTGTGC ATTGGACCTC CGATGATGAG TTCAGTGTGG TCTCCTACGA GCTCCAGTAC
    ACCATATTCA CCGGACAAGC CAACGTCGTT AGTCTGTGTA ATTCGGCTGA TAGCTGGATG
    ATAGTACCCA ACATCAAGCA GAACCACTAC ACGGTGCACG GTCTGCAGAG CGGCACCAAG
    TACATCTTCA TGGTCAAGGC CATCAACCAG GCGGGCAGCC GCAGCAGTGA GCCTGGGAAG
    TTGAAGACAA ACAGCCAACC ATTTAAACTG GATCCCAAAT CTGCTCATCG AAAACTGAAG
    GTGTCCCATG ATAACTTGAC AGTAGAACGT GATGAGTCAT CATCCAAGAA GAGTCACACA
    CCTGAACGCT TCACCAGCCA GGGGAGCTAT GGAGTAGCTG GAAATGTGTT TATTGATAGT
    GGCCGGCATT ATTGGGAAGT GGTCATAAGT GGAAGCACAT GGTATGCCAT TGGTCTTGCT
    TACAAATCAG CCCCGAAGCA TGAATGGATT GGGAAGAACT CTGCTTCCTG GGCGCTCTGC
    CGCTGCAACA ATAACTGGGT GGTGAGACAC AATAGCAAGG AAATCCCCAT TGAGCCTGCC
    CCCCACCTCC GGCGCGTGGG CATCTTGCTG GACTATGATA ACGGCTCTAT CGCCTTTTAT
    GATGCTTTGA ACTCCATCCA CCTCTACACC TTCGACGTCG CATTTGCGCA GCCTGTTTGC
    CCCACCTTCA CCGTGTGGAA CAAGTGTCTG ACGATTATCA CTGGGCTCCC TATCCCAGAC
    CATTTGGACT GCACAGAGCA GCTGCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016798233.1
    CDS567..2576
    Translation

    Target ORF information:

    RefSeq Version XM_016942744.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes midline 1 (MID1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016942744.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
    CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA
    CACTGTGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG
    CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG
    AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC
    CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG
    TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC
    TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT
    CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT
    GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG
    GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG
    ACTGGACTCA AAGGGAACCT GACATCTTCT CTGGAATTGA AGCCAAACAC AGAACTCTCA
    TGGAAGGAAG CAGAGGCACT GAGGATTTGC ATTTCTCTGG AAGTCAATGC ATCACGTCAA
    GAAGCCAAAT TGACAGAGGA GTGTGATCTT CTCATTGAGA TCATTCAGCA AAGACGACAG
    ATTATTGGAA CCAAGATCAA AGAAGGGAAG GTGATGAGGC TTCGCAAACT GGCTCAGCAG
    ATTGCAAACT GCAAACAGTG CATTGAGCGG TCAGCATCAC TCATCTCCCA AGCGGAACAC
    TCTCTGAAGG AGAATGATCA TGCGCGTTTC CTACAGACTG CTAAGAATAT CACCGAGAGA
    GTCTCCATGG CAACTGCATC CTCCCAGGTT CTAATTCCTG AAATCAACCT CAATGACACA
    TTTGACACCT TTGCCTTAGA TTTTTCCCGA GAGAAGAAAT TGCTAGAATG TCTGGATTAC
    CTTACAGCTC CCAACCCTCC CACAATTAGA GAAGAGCTCT GCACAGCTTC ATATGACACC
    ATCACTGTGC ATTGGACCTC CGATGATGAG TTCAGTGTGG TCTCCTACGA GCTCCAGTAC
    ACCATATTCA CCGGACAAGC CAACGTCGTT AGTCTGTGTA ATTCGGCTGA TAGCTGGATG
    ATAGTACCCA ACATCAAGCA GAACCACTAC ACGGTGCACG GTCTGCAGAG CGGCACCAAG
    TACATCTTCA TGGTCAAGGC CATCAACCAG GCGGGCAGCC GCAGCAGTGA GCCTGGGAAG
    TTGAAGACAA ACAGCCAACC ATTTAAACTG GATCCCAAAT CTGCTCATCG AAAACTGAAG
    GTGTCCCATG ATAACTTGAC AGTAGAACGT GATGAGTCAT CATCCAAGAA GAGTCACACA
    CCTGAACGCT TCACCAGCCA GGGGAGCTAT GGAGTAGCTG GAAATGTGTT TATTGATAGT
    GGCCGGCATT ATTGGGAAGT GGTCATAAGT GGAAGCACAT GGTATGCCAT TGGTCTTGCT
    TACAAATCAG CCCCGAAGCA TGAATGGATT GGGAAGAACT CTGCTTCCTG GGCGCTCTGC
    CGCTGCAACA ATAACTGGGT GGTGAGACAC AATAGCAAGG AAATCCCCAT TGAGCCTGCC
    CCCCACCTCC GGCGCGTGGG CATCTTGCTG GACTATGATA ACGGCTCTAT CGCCTTTTAT
    GATGCTTTGA ACTCCATCCA CCTCTACACC TTCGACGTCG CATTTGCGCA GCCTGTTTGC
    CCCACCTTCA CCGTGTGGAA CAAGTGTCTG ACGATTATCA CTGGGCTCCC TATCCCAGAC
    CATTTGGACT GCACAGAGCA GCTGCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.