F8 cDNA ORF clone, Pan troglodytes(chimpanzee)

The following F8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OPc91299 XM_016944627.2
Latest version!
Pan troglodytes coagulation factor VIII (F8), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
OPc91298 XM_016944626.2
Latest version!
Pan troglodytes coagulation factor VIII (F8), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OPc91296 XM_016944624.1
Latest version!
Pan troglodytes coagulation factor VIII (F8), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OPc91297 XM_016944625.2
Latest version!
Pan troglodytes coagulation factor VIII (F8), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OPc25432 XM_003317789.4
Latest version!
Pan troglodytes coagulation factor VIII (F8), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OPc46412 XM_009439870.2
Latest version!
Pan troglodytes coagulation factor VIII (F8), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OPc91300 XM_003317788.3
Latest version!
Pan troglodytes coagulation factor VIII (F8), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $174.30
$249.00
View Old Accession Versions >>
OPc91297 XM_016944625.1 Pan troglodytes coagulation factor VIII (F8), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OPc91299 XM_016944627.1 Pan troglodytes coagulation factor VIII (F8), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
OPc25432 XM_003317789.3 Pan troglodytes coagulation factor VIII (F8), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OPc91298 XM_016944626.1 Pan troglodytes coagulation factor VIII (F8), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OPc91299
    Clone ID Related Accession (Same CDS sequence) XM_016944627.2 , XM_016944627.1
    Accession Version XM_016944627.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product coagulation factor VIII isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036902.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016944627.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTTACCAT CCAAAGCTGG AATTTGGCGG GTGGAATGCC TTATTGGCGA GCATCTACAT 
    GCTGGGATGA GCACACTTTT TCTGGTGTAC AGCAATAAGT GTCAGACTCC CCTGGGAATG
    GCTTCTGGAC ACATTAGAGA TTTTCAGATT ACAGCTTCAG GACAATATGG ACAGTGGGCC
    CCAAAGCTGG CCAGACTTCA TTATTCCGGA TCAATCAATG CCTGGAGCAC CAAGGAGCCC
    TTTTCTTGGA TCAAGGTGGA TCTGTTGGCA CCAATGATTA TTCACGGCAT CAAGACCCAG
    GGTGCCCGTC AGAAGTTCTC CAGCCTCTAC ATCTCTCAGT TTATCATCAT GTATAGTCTT
    GATGGGAAGA AGTGGCAGAC TTATCGAGGA AATTCCACTG GAACCTTAAT GGTCTTCTTT
    GGCAATGTGG ATTCATCTGG GATAAAACAC AATATTTTTA ACCCTCCAAT TATTGCTCGA
    TACATCCGTT TGCACCCAAC TCATTATAGC ATTCGCAGCA CTCTTCGCAT GGAGTTGATG
    GGCTGTGATT TAAATAGTTG CAGCATGCCA TTGGGAATGG AGAGTAAAGC AATATCAGAT
    GCACAGATTA CTGCTTCATC CTACTTTACC AATATGTTTG CCACCTGGTC TCCTTCAAAA
    GCTCGACTTC ACCTCCAAGG GAGGAGTAAT GCCTGGAGAC CTCAGGTGAA TAATCCAAAA
    GAGTGGCTGC AAGTGGACTT CCAGAAGACA ATGAAAGTCA CAGGAATAAC TACTCAGGGA
    GTAAAATCTC TGCTTACCAG CATGTATGTG AAGGAGTTCC TCATCTCCAG CAGTCAAGAT
    GGCCATCACT GGACTCTCTT TTTTCAGAAT GGCAAAGTAA AGGTTTTTCA GGGAAATCAA
    GACTCCTTCA CACCTGTGGT GAACTCTCTA GACCCACCGT TACTGACTCG CTACCTTCGA
    ATTCACCCCC AGAGTTGGGT GCACCAGATT GCCCTGAGGA TGGAGGTTCT GGGCTGCGAG
    GCACAGGACC TCTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016800116.1
    CDS426..1463
    Translation

    Target ORF information:

    RefSeq Version XM_016944627.2
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes coagulation factor VIII (F8), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016944627.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTTACCAT CCAAAGCTGG AATTTGGCGG GTGGAATGCC TTATTGGCGA GCATCTACAT 
    GCTGGGATGA GCACACTTTT TCTGGTGTAC AGCAATAAGT GTCAGACTCC CCTGGGAATG
    GCTTCTGGAC ACATTAGAGA TTTTCAGATT ACAGCTTCAG GACAATATGG ACAGTGGGCC
    CCAAAGCTGG CCAGACTTCA TTATTCCGGA TCAATCAATG CCTGGAGCAC CAAGGAGCCC
    TTTTCTTGGA TCAAGGTGGA TCTGTTGGCA CCAATGATTA TTCACGGCAT CAAGACCCAG
    GGTGCCCGTC AGAAGTTCTC CAGCCTCTAC ATCTCTCAGT TTATCATCAT GTATAGTCTT
    GATGGGAAGA AGTGGCAGAC TTATCGAGGA AATTCCACTG GAACCTTAAT GGTCTTCTTT
    GGCAATGTGG ATTCATCTGG GATAAAACAC AATATTTTTA ACCCTCCAAT TATTGCTCGA
    TACATCCGTT TGCACCCAAC TCATTATAGC ATTCGCAGCA CTCTTCGCAT GGAGTTGATG
    GGCTGTGATT TAAATAGTTG CAGCATGCCA TTGGGAATGG AGAGTAAAGC AATATCAGAT
    GCACAGATTA CTGCTTCATC CTACTTTACC AATATGTTTG CCACCTGGTC TCCTTCAAAA
    GCTCGACTTC ACCTCCAAGG GAGGAGTAAT GCCTGGAGAC CTCAGGTGAA TAATCCAAAA
    GAGTGGCTGC AAGTGGACTT CCAGAAGACA ATGAAAGTCA CAGGAATAAC TACTCAGGGA
    GTAAAATCTC TGCTTACCAG CATGTATGTG AAGGAGTTCC TCATCTCCAG CAGTCAAGAT
    GGCCATCACT GGACTCTCTT TTTTCAGAAT GGCAAAGTAA AGGTTTTTCA GGGAAATCAA
    GACTCCTTCA CACCTGTGGT GAACTCTCTA GACCCACCGT TACTGACTCG CTACCTTCGA
    ATTCACCCCC AGAGTTGGGT GCACCAGATT GCCCTGAGGA TGGAGGTTCT GGGCTGCGAG
    GCACAGGACC TCTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc91299
    Clone ID Related Accession (Same CDS sequence) XM_016944627.2 , XM_016944627.1
    Accession Version XM_016944627.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product coagulation factor VIII isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006491.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTTACCAT CCAAAGCTGG AATTTGGCGG GTGGAATGCC TTATTGGCGA GCATCTACAT 
    GCTGGGATGA GCACACTTTT TCTGGTGTAC AGCAATAAGT GTCAGACTCC CCTGGGAATG
    GCTTCTGGAC ACATTAGAGA TTTTCAGATT ACAGCTTCAG GACAATATGG ACAGTGGGCC
    CCAAAGCTGG CCAGACTTCA TTATTCCGGA TCAATCAATG CCTGGAGCAC CAAGGAGCCC
    TTTTCTTGGA TCAAGGTGGA TCTGTTGGCA CCAATGATTA TTCACGGCAT CAAGACCCAG
    GGTGCCCGTC AGAAGTTCTC CAGCCTCTAC ATCTCTCAGT TTATCATCAT GTATAGTCTT
    GATGGGAAGA AGTGGCAGAC TTATCGAGGA AATTCCACTG GAACCTTAAT GGTCTTCTTT
    GGCAATGTGG ATTCATCTGG GATAAAACAC AATATTTTTA ACCCTCCAAT TATTGCTCGA
    TACATCCGTT TGCACCCAAC TCATTATAGC ATTCGCAGCA CTCTTCGCAT GGAGTTGATG
    GGCTGTGATT TAAATAGTTG CAGCATGCCA TTGGGAATGG AGAGTAAAGC AATATCAGAT
    GCACAGATTA CTGCTTCATC CTACTTTACC AATATGTTTG CCACCTGGTC TCCTTCAAAA
    GCTCGACTTC ACCTCCAAGG GAGGAGTAAT GCCTGGAGAC CTCAGGTGAA TAATCCAAAA
    GAGTGGCTGC AAGTGGACTT CCAGAAGACA ATGAAAGTCA CAGGAATAAC TACTCAGGGA
    GTAAAATCTC TGCTTACCAG CATGTATGTG AAGGAGTTCC TCATCTCCAG CAGTCAAGAT
    GGCCATCACT GGACTCTCTT TTTTCAGAAT GGCAAAGTAA AGGTTTTTCA GGGAAATCAA
    GACTCCTTCA CACCTGTGGT GAACTCTCTA GACCCACCGT TACTGACTCG CTACCTTCGA
    ATTCACCCCC AGAGTTGGGT GCACCAGATT GCCCTGAGGA TGGAGGTTCT GGGCTGCGAG
    GCACAGGACC TCTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016800116.1
    CDS425..1462
    Translation

    Target ORF information:

    RefSeq Version XM_016944627.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes coagulation factor VIII (F8), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016944627.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTTACCAT CCAAAGCTGG AATTTGGCGG GTGGAATGCC TTATTGGCGA GCATCTACAT 
    GCTGGGATGA GCACACTTTT TCTGGTGTAC AGCAATAAGT GTCAGACTCC CCTGGGAATG
    GCTTCTGGAC ACATTAGAGA TTTTCAGATT ACAGCTTCAG GACAATATGG ACAGTGGGCC
    CCAAAGCTGG CCAGACTTCA TTATTCCGGA TCAATCAATG CCTGGAGCAC CAAGGAGCCC
    TTTTCTTGGA TCAAGGTGGA TCTGTTGGCA CCAATGATTA TTCACGGCAT CAAGACCCAG
    GGTGCCCGTC AGAAGTTCTC CAGCCTCTAC ATCTCTCAGT TTATCATCAT GTATAGTCTT
    GATGGGAAGA AGTGGCAGAC TTATCGAGGA AATTCCACTG GAACCTTAAT GGTCTTCTTT
    GGCAATGTGG ATTCATCTGG GATAAAACAC AATATTTTTA ACCCTCCAAT TATTGCTCGA
    TACATCCGTT TGCACCCAAC TCATTATAGC ATTCGCAGCA CTCTTCGCAT GGAGTTGATG
    GGCTGTGATT TAAATAGTTG CAGCATGCCA TTGGGAATGG AGAGTAAAGC AATATCAGAT
    GCACAGATTA CTGCTTCATC CTACTTTACC AATATGTTTG CCACCTGGTC TCCTTCAAAA
    GCTCGACTTC ACCTCCAAGG GAGGAGTAAT GCCTGGAGAC CTCAGGTGAA TAATCCAAAA
    GAGTGGCTGC AAGTGGACTT CCAGAAGACA ATGAAAGTCA CAGGAATAAC TACTCAGGGA
    GTAAAATCTC TGCTTACCAG CATGTATGTG AAGGAGTTCC TCATCTCCAG CAGTCAAGAT
    GGCCATCACT GGACTCTCTT TTTTCAGAAT GGCAAAGTAA AGGTTTTTCA GGGAAATCAA
    GACTCCTTCA CACCTGTGGT GAACTCTCTA GACCCACCGT TACTGACTCG CTACCTTCGA
    ATTCACCCCC AGAGTTGGGT GCACCAGATT GCCCTGAGGA TGGAGGTTCT GGGCTGCGAG
    GCACAGGACC TCTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc91298
    Clone ID Related Accession (Same CDS sequence) XM_016944626.1 , XM_016944626.2
    Accession Version XM_016944626.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5016bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product coagulation factor VIII isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006491.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGTCTATG AAGATACACT CACCCTATTC CCATTCTCAG GAGAAACTGT CTTCATGTCG 
    ATGGAAAACC CAGGCCTATG GATTCTGGGG TGCCACAACT CAGACTTTCG GAACAGAGGC
    ATGACCGCCT TACTGAAGGT TTCTAGTTGT GACAAGAACA CTGGTGATTA TTACGAGGAC
    AGTTATGAAG ATATTTCAGC ATACTTGCTG AGTAAAAACA ATGCCATTGA ACCAAGAAGC
    TTCTCCCAGA ATTCAAGACA CCCTAGCACT AGGCAAAAGC AATTTAATGC CACCACAATT
    CCAGAAAATG ACATAGAGAA GACTGACCCT TGGTTTGCAC ACAGAACACC TATGCCTAAA
    GTACAAAATG TCTCCTCTAG TGATTTGTTG ATGCTCTTGC GACAGAGTCC TACTCCACAT
    GGGCTATCCT TATCTGATCT CCAAGAAGCC AAATATGAGA CTTTTTCTGA TGATCCATCA
    CCTGGAGCAA TAGACAGTAA TAACAGCCTA TCTGAAATGA CACACTTCAG GCCACAGTTC
    CATCACAGTG GGGACATGGT ATTTACCCCT GAGTCAGGCC TCCAATTGAG ATTAAATGAG
    AAACTGGGGA CAACTGCAGC AACAGAGTTG AAGAAACTTG ATTTCAAAGT TTCTAGTACA
    TCAAATAATC TGATTTCAAC AATTCCATCA GACAATTTGG CAGCAGGTAC TGATAATACA
    AGTTCCTTAG GACCCCCAAG TATGCCAGTT CATTATGATA GTCAATTAGA TACCACTCTA
    TTTGGCAAAA AGTCATCTCC CCTTACTGAG TCTGGTAGAC CTCTGAGCTT GAGTGAAGAA
    AATAATGATT CAAAGTTGTT AGAATCAGGT TTAATGAATA GCCAAGAAAG TTCATGGGGA
    AAAAATGTAT CGTCAACAGA GAGTGGTAGG TTATTTAAAG GGAAAAGAGC TCATGGACCT
    GCTTTGTTGA CTAAAGATAA TGCCTTATTC AAAGTTAGCA TCTCTTTGTT AAAGACAAAC
    AAAACTTCCA ATAATTCAGC AACTAATAGA AAGACTCACA TTGATGGCCC ATCATTATTA
    ATTCAGAGTA GTCCATCAGT CTGGCAAAAT ATATTAGAAA GTGACACTGA GTTTAAAAAA
    GTGACACCTT TGATTCATGA CAGAATGCTT ATGGACAAAA ATGCTACAGC TTTGAGGCTA
    AATCATATGT CAAATAAAAC TACTTCATCA AAAAACATGG AAATGGTCCG ACAGAAAAAA
    GAGGGCCCCA TTCCACCAGA TGCAGAAAAT CCAGATATGT CATTCTTTAA GATGCTATTC
    TTGCCAGAAT CAGCAAGTTG GATACAAAGG ACTCATGGAA AGAACTCTCT GAACTCTGGG
    CAAGGCCCCA GTCCAAAGCA ATTAGTATCC TTAGGACCAG AAAAATCTGT GGAAGGTCAG
    AATTTCTTGT CTGAGAAAAA CAAAGTGGTA GTAGGAAAGG GTGAATTTAC AAAGGACGTA
    GGACTCAAAG AGATGGTTTT TCCAAGCAGC AGAAACCTAT TTCTTACTAA CTTGGATAAT
    TTACATGAAA ATAATACACA CAATCAAGAA AAAAAAATTC AGGAAGAAAT AGAAAGGAAG
    GAAACATTAA TCCAAGAGAA TGTAGTTTTG CCTCAGATAC ATACAGTGAC TGGCACTAAG
    AATTTCATGA AGAACCTTTT CTTACTGAGC ACTAGGCAAA ATGTAGAAGG TTCATATGAG
    GGGGCATATG CTCCAGTACT TCAAGATTTT AGGTCATTAA ATGATTCAAC AAATAGAACA
    AAGAAACACA CGGCTCATTT CTCAAAAAAA GGGGAAGAAG AAAACTTGGC AGGCTTGGGA
    AATCAAACCA AGCAAATTGT AGAGAAATAT GCATGCACCA CAAGGATATC TCCTAATACA
    AGCCAGCAGA ATTTTGTCAC GCAACGTAGT AAGAGAGCTT TGAAACAATT CAGACTCCCA
    CTAGAAGAAA CAGAACTTGA AAAAAGGATA ATTGTGGATG ACACCTCAAC CCAGTGGTCC
    AAAAACATGA AACATTTGAC CCCGAGCACC CTCACACAGA TAGACTACAA TGAGAAGGAG
    AAAGGGGCCA TTACTCAGTC TCCCTTATCA GATTGCCTTA CGAGGAGTCA TAGCATCCCT
    CAAGCAAATA GATCTCCATT ACCCATTGCA GAGGTATCAT CATTTCCATC TATTAGACCT
    ATATATCTGA CCAGGGTCCT ATTCCAAGAC AACTCTTCTC ATCTTCCAGC AGCATCTTAT
    AGAAAGAAAG ATTCTGGGGT CCAAGAAAGC AGTCATTTCT TACAAGGAGC CAAAAAAAAT
    AACCTTTCTT TAGCCATTCT AACCTTGGAG ATGACTGGTG ATCAAAGAGA GGTTGGCTCC
    CTGGGGACAA GTGCCACAAA TTCAGTCACT TACAAGAAAG TTGAGAACAC TGTTCTCCCG
    AAACCAGGCT TGCCCAAAAC ATCTGGCAAA GTTGAATTGC TTCCAAAAGT TCACATTTAT
    CAGAAGGACC TATTCCCTAC GGAAACTAGC AATGGGTCTC CTGGCCATCT GGATCTCGTG
    GAAGGGAGCC TTCTTCAGGA AACAGAGGGA GCGATTAAGT GGAATGAAGC AAACAGACCT
    GGAAAAATTC CCTTTCTGAG AGTAGCAACA GAAAGCTCTG CAAAGACTCC CTCCAAGCTA
    TTGGATCCTC TTGCTTGGGA TAACCACTAT GGTACTCAGA TACCAAAAGA AGAGTGGAAA
    TCCCAAGAGA AGTCACCAGA AAAAACAGCT TTTAAGAAAA AGGATACCAT TTTGTCCCTG
    AACGCTTGTG AAAGCAATCA TGCAATAGCA GCAATAAATG AGGGACAAAA TAAGCCCGAA
    ATAGAAGTCA CCTGGGCAAA GCAAGGTAGG ACTGAAAGGC TGTGCTCTCA AAACCCACCA
    GGCTTGAAAC GCCATCAACG GGAAATAACT CGTACTACTC TTCAGTCAGA TCAAGAGGAA
    ATTGACTATG ATGATACCAT CTCAGTTGAA ATGAAGAAGG AAGATTTTGA CATTTATGAT
    GAGGATGAAA ATCAGAGCCC CCGCAGCTTT CAAAAGAAAA CACGACACTA TTTTATTGCT
    GCAGTGGAGA GGCTCTGGGA TTATGGGATG AGTAGCTCCC CACATGTTCT AAGAAACAGG
    GCTCAGAGTG GCAGTGTCCC TCAGTTCAAG AAAGTTGTTT TCCAGGAATT TACTGATGGC
    TCCTTTACTC AGCCCTTATA CCGTGGAGAA CTAAATGAAC ATTTGGGACT CCTGGGGCCA
    TATATAAGAG CAGAAGTTGA AGATAATATC ATGGTAACTT TCAAAAATCA GGCCTCTCGT
    CCCTATTCCT TCTATTCTAG CCTTATTTCT TATGAGGAAG ATCAGAGGCA AGGAGCAGAA
    CCTAGGAAAA ACTTTGTCAA GCCTAATGAA ACCAAAACTT ACTTTTGGAA AGTGCAACAT
    CATATGGCGC CCACTAAAGA TGAGTTTGAC TGCAAAGCCT GGGCTTATTT CTCTGATGTT
    GACCTGGAAA AAGATGTGCA CTCAGGCCTG ATTGGACCCC TTCTGGTCTG CCACACTAAC
    ACACTGAACC CTGCTCATGG GAGACAAGTG ACAGTACAGG AATTTGCTCT GTTTTTCACC
    ATCTTTGATG AGACCAAAAG CTGGTACTTC ACTGAAAACA TGGAAAGAAA CTGCAGGGCT
    CCCTGCAATA TCCAGATGGA AGATCCCACT TTTAAAGAGA ATTATCGCTT CCATGCAATC
    AATGGCTACA TAATGGATAC ACTACCTGGC TTAGTAATGG CTCAGGATCA AAGGATTCGA
    TGGTATCTGC TCAGCATGGG CAGCAATGAA AACATCCATT CTATTCATTT CAGTGGACAT
    GTGTTCACTG TACGAAAAAA AGAGGAGTAT AAAATGGCAC TGTACAATCT CTATCCAGGT
    GTTTTTGAGA CAGTGGAAAT GTTACCATCC AAAGCTGGAA TTTGGCGGGT GGAATGCCTT
    ATTGGCGAGC ATCTACATGC TGGGATGAGC ACACTTTTTC TGGTGTACAG CAATAAGTGT
    CAGACTCCCC TGGGAATGGC TTCTGGACAC ATTAGAGATT TTCAGATTAC AGCTTCAGGA
    CAATATGGAC AGTGGGCCCC AAAGCTGGCC AGACTTCATT ATTCCGGATC AATCAATGCC
    TGGAGCACCA AGGAGCCCTT TTCTTGGATC AAGGTGGATC TGTTGGCACC AATGATTATT
    CACGGCATCA AGACCCAGGG TGCCCGTCAG AAGTTCTCCA GCCTCTACAT CTCTCAGTTT
    ATCATCATGT ATAGTCTTGA TGGGAAGAAG TGGCAGACTT ATCGAGGAAA TTCCACTGGA
    ACCTTAATGG TCTTCTTTGG CAATGTGGAT TCATCTGGGA TAAAACACAA TATTTTTAAC
    CCTCCAATTA TTGCTCGATA CATCCGTTTG CACCCAACTC ATTATAGCAT TCGCAGCACT
    CTTCGCATGG AGTTGATGGG CTGTGATTTA AATAGTTGCA GCATGCCATT GGGAATGGAG
    AGTAAAGCAA TATCAGATGC ACAGATTACT GCTTCATCCT ACTTTACCAA TATGTTTGCC
    ACCTGGTCTC CTTCAAAAGC TCGACTTCAC CTCCAAGGGA GGAGTAATGC CTGGAGACCT
    CAGGTGAATA ATCCAAAAGA GTGGCTGCAA GTGGACTTCC AGAAGACAAT GAAAGTCACA
    GGAATAACTA CTCAGGGAGT AAAATCTCTG CTTACCAGCA TGTATGTGAA GGAGTTCCTC
    ATCTCCAGCA GTCAAGATGG CCATCACTGG ACTCTCTTTT TTCAGAATGG CAAAGTAAAG
    GTTTTTCAGG GAAATCAAGA CTCCTTCACA CCTGTGGTGA ACTCTCTAGA CCCACCGTTA
    CTGACTCGCT ACCTTCGAAT TCACCCCCAG AGTTGGGTGC ACCAGATTGC CCTGAGGATG
    GAGGTTCTGG GCTGCGAGGC ACAGGACCTC TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016800115.1
    CDS427..5442
    Translation

    Target ORF information:

    RefSeq Version XM_016944626.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes coagulation factor VIII (F8), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016944626.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGTCTATG AAGATACACT CACCCTATTC CCATTCTCAG GAGAAACTGT CTTCATGTCG 
    ATGGAAAACC CAGGCCTATG GATTCTGGGG TGCCACAACT CAGACTTTCG GAACAGAGGC
    ATGACCGCCT TACTGAAGGT TTCTAGTTGT GACAAGAACA CTGGTGATTA TTACGAGGAC
    AGTTATGAAG ATATTTCAGC ATACTTGCTG AGTAAAAACA ATGCCATTGA ACCAAGAAGC
    TTCTCCCAGA ATTCAAGACA CCCTAGCACT AGGCAAAAGC AATTTAATGC CACCACAATT
    CCAGAAAATG ACATAGAGAA GACTGACCCT TGGTTTGCAC ACAGAACACC TATGCCTAAA
    GTACAAAATG TCTCCTCTAG TGATTTGTTG ATGCTCTTGC GACAGAGTCC TACTCCACAT
    GGGCTATCCT TATCTGATCT CCAAGAAGCC AAATATGAGA CTTTTTCTGA TGATCCATCA
    CCTGGAGCAA TAGACAGTAA TAACAGCCTA TCTGAAATGA CACACTTCAG GCCACAGTTC
    CATCACAGTG GGGACATGGT ATTTACCCCT GAGTCAGGCC TCCAATTGAG ATTAAATGAG
    AAACTGGGGA CAACTGCAGC AACAGAGTTG AAGAAACTTG ATTTCAAAGT TTCTAGTACA
    TCAAATAATC TGATTTCAAC AATTCCATCA GACAATTTGG CAGCAGGTAC TGATAATACA
    AGTTCCTTAG GACCCCCAAG TATGCCAGTT CATTATGATA GTCAATTAGA TACCACTCTA
    TTTGGCAAAA AGTCATCTCC CCTTACTGAG TCTGGTAGAC CTCTGAGCTT GAGTGAAGAA
    AATAATGATT CAAAGTTGTT AGAATCAGGT TTAATGAATA GCCAAGAAAG TTCATGGGGA
    AAAAATGTAT CGTCAACAGA GAGTGGTAGG TTATTTAAAG GGAAAAGAGC TCATGGACCT
    GCTTTGTTGA CTAAAGATAA TGCCTTATTC AAAGTTAGCA TCTCTTTGTT AAAGACAAAC
    AAAACTTCCA ATAATTCAGC AACTAATAGA AAGACTCACA TTGATGGCCC ATCATTATTA
    ATTCAGAGTA GTCCATCAGT CTGGCAAAAT ATATTAGAAA GTGACACTGA GTTTAAAAAA
    GTGACACCTT TGATTCATGA CAGAATGCTT ATGGACAAAA ATGCTACAGC TTTGAGGCTA
    AATCATATGT CAAATAAAAC TACTTCATCA AAAAACATGG AAATGGTCCG ACAGAAAAAA
    GAGGGCCCCA TTCCACCAGA TGCAGAAAAT CCAGATATGT CATTCTTTAA GATGCTATTC
    TTGCCAGAAT CAGCAAGTTG GATACAAAGG ACTCATGGAA AGAACTCTCT GAACTCTGGG
    CAAGGCCCCA GTCCAAAGCA ATTAGTATCC TTAGGACCAG AAAAATCTGT GGAAGGTCAG
    AATTTCTTGT CTGAGAAAAA CAAAGTGGTA GTAGGAAAGG GTGAATTTAC AAAGGACGTA
    GGACTCAAAG AGATGGTTTT TCCAAGCAGC AGAAACCTAT TTCTTACTAA CTTGGATAAT
    TTACATGAAA ATAATACACA CAATCAAGAA AAAAAAATTC AGGAAGAAAT AGAAAGGAAG
    GAAACATTAA TCCAAGAGAA TGTAGTTTTG CCTCAGATAC ATACAGTGAC TGGCACTAAG
    AATTTCATGA AGAACCTTTT CTTACTGAGC ACTAGGCAAA ATGTAGAAGG TTCATATGAG
    GGGGCATATG CTCCAGTACT TCAAGATTTT AGGTCATTAA ATGATTCAAC AAATAGAACA
    AAGAAACACA CGGCTCATTT CTCAAAAAAA GGGGAAGAAG AAAACTTGGC AGGCTTGGGA
    AATCAAACCA AGCAAATTGT AGAGAAATAT GCATGCACCA CAAGGATATC TCCTAATACA
    AGCCAGCAGA ATTTTGTCAC GCAACGTAGT AAGAGAGCTT TGAAACAATT CAGACTCCCA
    CTAGAAGAAA CAGAACTTGA AAAAAGGATA ATTGTGGATG ACACCTCAAC CCAGTGGTCC
    AAAAACATGA AACATTTGAC CCCGAGCACC CTCACACAGA TAGACTACAA TGAGAAGGAG
    AAAGGGGCCA TTACTCAGTC TCCCTTATCA GATTGCCTTA CGAGGAGTCA TAGCATCCCT
    CAAGCAAATA GATCTCCATT ACCCATTGCA GAGGTATCAT CATTTCCATC TATTAGACCT
    ATATATCTGA CCAGGGTCCT ATTCCAAGAC AACTCTTCTC ATCTTCCAGC AGCATCTTAT
    AGAAAGAAAG ATTCTGGGGT CCAAGAAAGC AGTCATTTCT TACAAGGAGC CAAAAAAAAT
    AACCTTTCTT TAGCCATTCT AACCTTGGAG ATGACTGGTG ATCAAAGAGA GGTTGGCTCC
    CTGGGGACAA GTGCCACAAA TTCAGTCACT TACAAGAAAG TTGAGAACAC TGTTCTCCCG
    AAACCAGGCT TGCCCAAAAC ATCTGGCAAA GTTGAATTGC TTCCAAAAGT TCACATTTAT
    CAGAAGGACC TATTCCCTAC GGAAACTAGC AATGGGTCTC CTGGCCATCT GGATCTCGTG
    GAAGGGAGCC TTCTTCAGGA AACAGAGGGA GCGATTAAGT GGAATGAAGC AAACAGACCT
    GGAAAAATTC CCTTTCTGAG AGTAGCAACA GAAAGCTCTG CAAAGACTCC CTCCAAGCTA
    TTGGATCCTC TTGCTTGGGA TAACCACTAT GGTACTCAGA TACCAAAAGA AGAGTGGAAA
    TCCCAAGAGA AGTCACCAGA AAAAACAGCT TTTAAGAAAA AGGATACCAT TTTGTCCCTG
    AACGCTTGTG AAAGCAATCA TGCAATAGCA GCAATAAATG AGGGACAAAA TAAGCCCGAA
    ATAGAAGTCA CCTGGGCAAA GCAAGGTAGG ACTGAAAGGC TGTGCTCTCA AAACCCACCA
    GGCTTGAAAC GCCATCAACG GGAAATAACT CGTACTACTC TTCAGTCAGA TCAAGAGGAA
    ATTGACTATG ATGATACCAT CTCAGTTGAA ATGAAGAAGG AAGATTTTGA CATTTATGAT
    GAGGATGAAA ATCAGAGCCC CCGCAGCTTT CAAAAGAAAA CACGACACTA TTTTATTGCT
    GCAGTGGAGA GGCTCTGGGA TTATGGGATG AGTAGCTCCC CACATGTTCT AAGAAACAGG
    GCTCAGAGTG GCAGTGTCCC TCAGTTCAAG AAAGTTGTTT TCCAGGAATT TACTGATGGC
    TCCTTTACTC AGCCCTTATA CCGTGGAGAA CTAAATGAAC ATTTGGGACT CCTGGGGCCA
    TATATAAGAG CAGAAGTTGA AGATAATATC ATGGTAACTT TCAAAAATCA GGCCTCTCGT
    CCCTATTCCT TCTATTCTAG CCTTATTTCT TATGAGGAAG ATCAGAGGCA AGGAGCAGAA
    CCTAGGAAAA ACTTTGTCAA GCCTAATGAA ACCAAAACTT ACTTTTGGAA AGTGCAACAT
    CATATGGCGC CCACTAAAGA TGAGTTTGAC TGCAAAGCCT GGGCTTATTT CTCTGATGTT
    GACCTGGAAA AAGATGTGCA CTCAGGCCTG ATTGGACCCC TTCTGGTCTG CCACACTAAC
    ACACTGAACC CTGCTCATGG GAGACAAGTG ACAGTACAGG AATTTGCTCT GTTTTTCACC
    ATCTTTGATG AGACCAAAAG CTGGTACTTC ACTGAAAACA TGGAAAGAAA CTGCAGGGCT
    CCCTGCAATA TCCAGATGGA AGATCCCACT TTTAAAGAGA ATTATCGCTT CCATGCAATC
    AATGGCTACA TAATGGATAC ACTACCTGGC TTAGTAATGG CTCAGGATCA AAGGATTCGA
    TGGTATCTGC TCAGCATGGG CAGCAATGAA AACATCCATT CTATTCATTT CAGTGGACAT
    GTGTTCACTG TACGAAAAAA AGAGGAGTAT AAAATGGCAC TGTACAATCT CTATCCAGGT
    GTTTTTGAGA CAGTGGAAAT GTTACCATCC AAAGCTGGAA TTTGGCGGGT GGAATGCCTT
    ATTGGCGAGC ATCTACATGC TGGGATGAGC ACACTTTTTC TGGTGTACAG CAATAAGTGT
    CAGACTCCCC TGGGAATGGC TTCTGGACAC ATTAGAGATT TTCAGATTAC AGCTTCAGGA
    CAATATGGAC AGTGGGCCCC AAAGCTGGCC AGACTTCATT ATTCCGGATC AATCAATGCC
    TGGAGCACCA AGGAGCCCTT TTCTTGGATC AAGGTGGATC TGTTGGCACC AATGATTATT
    CACGGCATCA AGACCCAGGG TGCCCGTCAG AAGTTCTCCA GCCTCTACAT CTCTCAGTTT
    ATCATCATGT ATAGTCTTGA TGGGAAGAAG TGGCAGACTT ATCGAGGAAA TTCCACTGGA
    ACCTTAATGG TCTTCTTTGG CAATGTGGAT TCATCTGGGA TAAAACACAA TATTTTTAAC
    CCTCCAATTA TTGCTCGATA CATCCGTTTG CACCCAACTC ATTATAGCAT TCGCAGCACT
    CTTCGCATGG AGTTGATGGG CTGTGATTTA AATAGTTGCA GCATGCCATT GGGAATGGAG
    AGTAAAGCAA TATCAGATGC ACAGATTACT GCTTCATCCT ACTTTACCAA TATGTTTGCC
    ACCTGGTCTC CTTCAAAAGC TCGACTTCAC CTCCAAGGGA GGAGTAATGC CTGGAGACCT
    CAGGTGAATA ATCCAAAAGA GTGGCTGCAA GTGGACTTCC AGAAGACAAT GAAAGTCACA
    GGAATAACTA CTCAGGGAGT AAAATCTCTG CTTACCAGCA TGTATGTGAA GGAGTTCCTC
    ATCTCCAGCA GTCAAGATGG CCATCACTGG ACTCTCTTTT TTCAGAATGG CAAAGTAAAG
    GTTTTTCAGG GAAATCAAGA CTCCTTCACA CCTGTGGTGA ACTCTCTAGA CCCACCGTTA
    CTGACTCGCT ACCTTCGAAT TCACCCCCAG AGTTGGGTGC ACCAGATTGC CCTGAGGATG
    GAGGTTCTGG GCTGCGAGGC ACAGGACCTC TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc91298
    Clone ID Related Accession (Same CDS sequence) XM_016944626.1 , XM_016944626.2
    Accession Version XM_016944626.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5016bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product coagulation factor VIII isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036902.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016944626.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGTCTATG AAGATACACT CACCCTATTC CCATTCTCAG GAGAAACTGT CTTCATGTCG 
    ATGGAAAACC CAGGCCTATG GATTCTGGGG TGCCACAACT CAGACTTTCG GAACAGAGGC
    ATGACCGCCT TACTGAAGGT TTCTAGTTGT GACAAGAACA CTGGTGATTA TTACGAGGAC
    AGTTATGAAG ATATTTCAGC ATACTTGCTG AGTAAAAACA ATGCCATTGA ACCAAGAAGC
    TTCTCCCAGA ATTCAAGACA CCCTAGCACT AGGCAAAAGC AATTTAATGC CACCACAATT
    CCAGAAAATG ACATAGAGAA GACTGACCCT TGGTTTGCAC ACAGAACACC TATGCCTAAA
    GTACAAAATG TCTCCTCTAG TGATTTGTTG ATGCTCTTGC GACAGAGTCC TACTCCACAT
    GGGCTATCCT TATCTGATCT CCAAGAAGCC AAATATGAGA CTTTTTCTGA TGATCCATCA
    CCTGGAGCAA TAGACAGTAA TAACAGCCTA TCTGAAATGA CACACTTCAG GCCACAGTTC
    CATCACAGTG GGGACATGGT ATTTACCCCT GAGTCAGGCC TCCAATTGAG ATTAAATGAG
    AAACTGGGGA CAACTGCAGC AACAGAGTTG AAGAAACTTG ATTTCAAAGT TTCTAGTACA
    TCAAATAATC TGATTTCAAC AATTCCATCA GACAATTTGG CAGCAGGTAC TGATAATACA
    AGTTCCTTAG GACCCCCAAG TATGCCAGTT CATTATGATA GTCAATTAGA TACCACTCTA
    TTTGGCAAAA AGTCATCTCC CCTTACTGAG TCTGGTAGAC CTCTGAGCTT GAGTGAAGAA
    AATAATGATT CAAAGTTGTT AGAATCAGGT TTAATGAATA GCCAAGAAAG TTCATGGGGA
    AAAAATGTAT CGTCAACAGA GAGTGGTAGG TTATTTAAAG GGAAAAGAGC TCATGGACCT
    GCTTTGTTGA CTAAAGATAA TGCCTTATTC AAAGTTAGCA TCTCTTTGTT AAAGACAAAC
    AAAACTTCCA ATAATTCAGC AACTAATAGA AAGACTCACA TTGATGGCCC ATCATTATTA
    ATTCAGAGTA GTCCATCAGT CTGGCAAAAT ATATTAGAAA GTGACACTGA GTTTAAAAAA
    GTGACACCTT TGATTCATGA CAGAATGCTT ATGGACAAAA ATGCTACAGC TTTGAGGCTA
    AATCATATGT CAAATAAAAC TACTTCATCA AAAAACATGG AAATGGTCCG ACAGAAAAAA
    GAGGGCCCCA TTCCACCAGA TGCAGAAAAT CCAGATATGT CATTCTTTAA GATGCTATTC
    TTGCCAGAAT CAGCAAGTTG GATACAAAGG ACTCATGGAA AGAACTCTCT GAACTCTGGG
    CAAGGCCCCA GTCCAAAGCA ATTAGTATCC TTAGGACCAG AAAAATCTGT GGAAGGTCAG
    AATTTCTTGT CTGAGAAAAA CAAAGTGGTA GTAGGAAAGG GTGAATTTAC AAAGGACGTA
    GGACTCAAAG AGATGGTTTT TCCAAGCAGC AGAAACCTAT TTCTTACTAA CTTGGATAAT
    TTACATGAAA ATAATACACA CAATCAAGAA AAAAAAATTC AGGAAGAAAT AGAAAGGAAG
    GAAACATTAA TCCAAGAGAA TGTAGTTTTG CCTCAGATAC ATACAGTGAC TGGCACTAAG
    AATTTCATGA AGAACCTTTT CTTACTGAGC ACTAGGCAAA ATGTAGAAGG TTCATATGAG
    GGGGCATATG CTCCAGTACT TCAAGATTTT AGGTCATTAA ATGATTCAAC AAATAGAACA
    AAGAAACACA CGGCTCATTT CTCAAAAAAA GGGGAAGAAG AAAACTTGGC AGGCTTGGGA
    AATCAAACCA AGCAAATTGT AGAGAAATAT GCATGCACCA CAAGGATATC TCCTAATACA
    AGCCAGCAGA ATTTTGTCAC GCAACGTAGT AAGAGAGCTT TGAAACAATT CAGACTCCCA
    CTAGAAGAAA CAGAACTTGA AAAAAGGATA ATTGTGGATG ACACCTCAAC CCAGTGGTCC
    AAAAACATGA AACATTTGAC CCCGAGCACC CTCACACAGA TAGACTACAA TGAGAAGGAG
    AAAGGGGCCA TTACTCAGTC TCCCTTATCA GATTGCCTTA CGAGGAGTCA TAGCATCCCT
    CAAGCAAATA GATCTCCATT ACCCATTGCA GAGGTATCAT CATTTCCATC TATTAGACCT
    ATATATCTGA CCAGGGTCCT ATTCCAAGAC AACTCTTCTC ATCTTCCAGC AGCATCTTAT
    AGAAAGAAAG ATTCTGGGGT CCAAGAAAGC AGTCATTTCT TACAAGGAGC CAAAAAAAAT
    AACCTTTCTT TAGCCATTCT AACCTTGGAG ATGACTGGTG ATCAAAGAGA GGTTGGCTCC
    CTGGGGACAA GTGCCACAAA TTCAGTCACT TACAAGAAAG TTGAGAACAC TGTTCTCCCG
    AAACCAGGCT TGCCCAAAAC ATCTGGCAAA GTTGAATTGC TTCCAAAAGT TCACATTTAT
    CAGAAGGACC TATTCCCTAC GGAAACTAGC AATGGGTCTC CTGGCCATCT GGATCTCGTG
    GAAGGGAGCC TTCTTCAGGA AACAGAGGGA GCGATTAAGT GGAATGAAGC AAACAGACCT
    GGAAAAATTC CCTTTCTGAG AGTAGCAACA GAAAGCTCTG CAAAGACTCC CTCCAAGCTA
    TTGGATCCTC TTGCTTGGGA TAACCACTAT GGTACTCAGA TACCAAAAGA AGAGTGGAAA
    TCCCAAGAGA AGTCACCAGA AAAAACAGCT TTTAAGAAAA AGGATACCAT TTTGTCCCTG
    AACGCTTGTG AAAGCAATCA TGCAATAGCA GCAATAAATG AGGGACAAAA TAAGCCCGAA
    ATAGAAGTCA CCTGGGCAAA GCAAGGTAGG ACTGAAAGGC TGTGCTCTCA AAACCCACCA
    GGCTTGAAAC GCCATCAACG GGAAATAACT CGTACTACTC TTCAGTCAGA TCAAGAGGAA
    ATTGACTATG ATGATACCAT CTCAGTTGAA ATGAAGAAGG AAGATTTTGA CATTTATGAT
    GAGGATGAAA ATCAGAGCCC CCGCAGCTTT CAAAAGAAAA CACGACACTA TTTTATTGCT
    GCAGTGGAGA GGCTCTGGGA TTATGGGATG AGTAGCTCCC CACATGTTCT AAGAAACAGG
    GCTCAGAGTG GCAGTGTCCC TCAGTTCAAG AAAGTTGTTT TCCAGGAATT TACTGATGGC
    TCCTTTACTC AGCCCTTATA CCGTGGAGAA CTAAATGAAC ATTTGGGACT CCTGGGGCCA
    TATATAAGAG CAGAAGTTGA AGATAATATC ATGGTAACTT TCAAAAATCA GGCCTCTCGT
    CCCTATTCCT TCTATTCTAG CCTTATTTCT TATGAGGAAG ATCAGAGGCA AGGAGCAGAA
    CCTAGGAAAA ACTTTGTCAA GCCTAATGAA ACCAAAACTT ACTTTTGGAA AGTGCAACAT
    CATATGGCGC CCACTAAAGA TGAGTTTGAC TGCAAAGCCT GGGCTTATTT CTCTGATGTT
    GACCTGGAAA AAGATGTGCA CTCAGGCCTG ATTGGACCCC TTCTGGTCTG CCACACTAAC
    ACACTGAACC CTGCTCATGG GAGACAAGTG ACAGTACAGG AATTTGCTCT GTTTTTCACC
    ATCTTTGATG AGACCAAAAG CTGGTACTTC ACTGAAAACA TGGAAAGAAA CTGCAGGGCT
    CCCTGCAATA TCCAGATGGA AGATCCCACT TTTAAAGAGA ATTATCGCTT CCATGCAATC
    AATGGCTACA TAATGGATAC ACTACCTGGC TTAGTAATGG CTCAGGATCA AAGGATTCGA
    TGGTATCTGC TCAGCATGGG CAGCAATGAA AACATCCATT CTATTCATTT CAGTGGACAT
    GTGTTCACTG TACGAAAAAA AGAGGAGTAT AAAATGGCAC TGTACAATCT CTATCCAGGT
    GTTTTTGAGA CAGTGGAAAT GTTACCATCC AAAGCTGGAA TTTGGCGGGT GGAATGCCTT
    ATTGGCGAGC ATCTACATGC TGGGATGAGC ACACTTTTTC TGGTGTACAG CAATAAGTGT
    CAGACTCCCC TGGGAATGGC TTCTGGACAC ATTAGAGATT TTCAGATTAC AGCTTCAGGA
    CAATATGGAC AGTGGGCCCC AAAGCTGGCC AGACTTCATT ATTCCGGATC AATCAATGCC
    TGGAGCACCA AGGAGCCCTT TTCTTGGATC AAGGTGGATC TGTTGGCACC AATGATTATT
    CACGGCATCA AGACCCAGGG TGCCCGTCAG AAGTTCTCCA GCCTCTACAT CTCTCAGTTT
    ATCATCATGT ATAGTCTTGA TGGGAAGAAG TGGCAGACTT ATCGAGGAAA TTCCACTGGA
    ACCTTAATGG TCTTCTTTGG CAATGTGGAT TCATCTGGGA TAAAACACAA TATTTTTAAC
    CCTCCAATTA TTGCTCGATA CATCCGTTTG CACCCAACTC ATTATAGCAT TCGCAGCACT
    CTTCGCATGG AGTTGATGGG CTGTGATTTA AATAGTTGCA GCATGCCATT GGGAATGGAG
    AGTAAAGCAA TATCAGATGC ACAGATTACT GCTTCATCCT ACTTTACCAA TATGTTTGCC
    ACCTGGTCTC CTTCAAAAGC TCGACTTCAC CTCCAAGGGA GGAGTAATGC CTGGAGACCT
    CAGGTGAATA ATCCAAAAGA GTGGCTGCAA GTGGACTTCC AGAAGACAAT GAAAGTCACA
    GGAATAACTA CTCAGGGAGT AAAATCTCTG CTTACCAGCA TGTATGTGAA GGAGTTCCTC
    ATCTCCAGCA GTCAAGATGG CCATCACTGG ACTCTCTTTT TTCAGAATGG CAAAGTAAAG
    GTTTTTCAGG GAAATCAAGA CTCCTTCACA CCTGTGGTGA ACTCTCTAGA CCCACCGTTA
    CTGACTCGCT ACCTTCGAAT TCACCCCCAG AGTTGGGTGC ACCAGATTGC CCTGAGGATG
    GAGGTTCTGG GCTGCGAGGC ACAGGACCTC TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016800115.1
    CDS419..5434
    Translation

    Target ORF information:

    RefSeq Version XM_016944626.2
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes coagulation factor VIII (F8), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016944626.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGTCTATG AAGATACACT CACCCTATTC CCATTCTCAG GAGAAACTGT CTTCATGTCG 
    ATGGAAAACC CAGGCCTATG GATTCTGGGG TGCCACAACT CAGACTTTCG GAACAGAGGC
    ATGACCGCCT TACTGAAGGT TTCTAGTTGT GACAAGAACA CTGGTGATTA TTACGAGGAC
    AGTTATGAAG ATATTTCAGC ATACTTGCTG AGTAAAAACA ATGCCATTGA ACCAAGAAGC
    TTCTCCCAGA ATTCAAGACA CCCTAGCACT AGGCAAAAGC AATTTAATGC CACCACAATT
    CCAGAAAATG ACATAGAGAA GACTGACCCT TGGTTTGCAC ACAGAACACC TATGCCTAAA
    GTACAAAATG TCTCCTCTAG TGATTTGTTG ATGCTCTTGC GACAGAGTCC TACTCCACAT
    GGGCTATCCT TATCTGATCT CCAAGAAGCC AAATATGAGA CTTTTTCTGA TGATCCATCA
    CCTGGAGCAA TAGACAGTAA TAACAGCCTA TCTGAAATGA CACACTTCAG GCCACAGTTC
    CATCACAGTG GGGACATGGT ATTTACCCCT GAGTCAGGCC TCCAATTGAG ATTAAATGAG
    AAACTGGGGA CAACTGCAGC AACAGAGTTG AAGAAACTTG ATTTCAAAGT TTCTAGTACA
    TCAAATAATC TGATTTCAAC AATTCCATCA GACAATTTGG CAGCAGGTAC TGATAATACA
    AGTTCCTTAG GACCCCCAAG TATGCCAGTT CATTATGATA GTCAATTAGA TACCACTCTA
    TTTGGCAAAA AGTCATCTCC CCTTACTGAG TCTGGTAGAC CTCTGAGCTT GAGTGAAGAA
    AATAATGATT CAAAGTTGTT AGAATCAGGT TTAATGAATA GCCAAGAAAG TTCATGGGGA
    AAAAATGTAT CGTCAACAGA GAGTGGTAGG TTATTTAAAG GGAAAAGAGC TCATGGACCT
    GCTTTGTTGA CTAAAGATAA TGCCTTATTC AAAGTTAGCA TCTCTTTGTT AAAGACAAAC
    AAAACTTCCA ATAATTCAGC AACTAATAGA AAGACTCACA TTGATGGCCC ATCATTATTA
    ATTCAGAGTA GTCCATCAGT CTGGCAAAAT ATATTAGAAA GTGACACTGA GTTTAAAAAA
    GTGACACCTT TGATTCATGA CAGAATGCTT ATGGACAAAA ATGCTACAGC TTTGAGGCTA
    AATCATATGT CAAATAAAAC TACTTCATCA AAAAACATGG AAATGGTCCG ACAGAAAAAA
    GAGGGCCCCA TTCCACCAGA TGCAGAAAAT CCAGATATGT CATTCTTTAA GATGCTATTC
    TTGCCAGAAT CAGCAAGTTG GATACAAAGG ACTCATGGAA AGAACTCTCT GAACTCTGGG
    CAAGGCCCCA GTCCAAAGCA ATTAGTATCC TTAGGACCAG AAAAATCTGT GGAAGGTCAG
    AATTTCTTGT CTGAGAAAAA CAAAGTGGTA GTAGGAAAGG GTGAATTTAC AAAGGACGTA
    GGACTCAAAG AGATGGTTTT TCCAAGCAGC AGAAACCTAT TTCTTACTAA CTTGGATAAT
    TTACATGAAA ATAATACACA CAATCAAGAA AAAAAAATTC AGGAAGAAAT AGAAAGGAAG
    GAAACATTAA TCCAAGAGAA TGTAGTTTTG CCTCAGATAC ATACAGTGAC TGGCACTAAG
    AATTTCATGA AGAACCTTTT CTTACTGAGC ACTAGGCAAA ATGTAGAAGG TTCATATGAG
    GGGGCATATG CTCCAGTACT TCAAGATTTT AGGTCATTAA ATGATTCAAC AAATAGAACA
    AAGAAACACA CGGCTCATTT CTCAAAAAAA GGGGAAGAAG AAAACTTGGC AGGCTTGGGA
    AATCAAACCA AGCAAATTGT AGAGAAATAT GCATGCACCA CAAGGATATC TCCTAATACA
    AGCCAGCAGA ATTTTGTCAC GCAACGTAGT AAGAGAGCTT TGAAACAATT CAGACTCCCA
    CTAGAAGAAA CAGAACTTGA AAAAAGGATA ATTGTGGATG ACACCTCAAC CCAGTGGTCC
    AAAAACATGA AACATTTGAC CCCGAGCACC CTCACACAGA TAGACTACAA TGAGAAGGAG
    AAAGGGGCCA TTACTCAGTC TCCCTTATCA GATTGCCTTA CGAGGAGTCA TAGCATCCCT
    CAAGCAAATA GATCTCCATT ACCCATTGCA GAGGTATCAT CATTTCCATC TATTAGACCT
    ATATATCTGA CCAGGGTCCT ATTCCAAGAC AACTCTTCTC ATCTTCCAGC AGCATCTTAT
    AGAAAGAAAG ATTCTGGGGT CCAAGAAAGC AGTCATTTCT TACAAGGAGC CAAAAAAAAT
    AACCTTTCTT TAGCCATTCT AACCTTGGAG ATGACTGGTG ATCAAAGAGA GGTTGGCTCC
    CTGGGGACAA GTGCCACAAA TTCAGTCACT TACAAGAAAG TTGAGAACAC TGTTCTCCCG
    AAACCAGGCT TGCCCAAAAC ATCTGGCAAA GTTGAATTGC TTCCAAAAGT TCACATTTAT
    CAGAAGGACC TATTCCCTAC GGAAACTAGC AATGGGTCTC CTGGCCATCT GGATCTCGTG
    GAAGGGAGCC TTCTTCAGGA AACAGAGGGA GCGATTAAGT GGAATGAAGC AAACAGACCT
    GGAAAAATTC CCTTTCTGAG AGTAGCAACA GAAAGCTCTG CAAAGACTCC CTCCAAGCTA
    TTGGATCCTC TTGCTTGGGA TAACCACTAT GGTACTCAGA TACCAAAAGA AGAGTGGAAA
    TCCCAAGAGA AGTCACCAGA AAAAACAGCT TTTAAGAAAA AGGATACCAT TTTGTCCCTG
    AACGCTTGTG AAAGCAATCA TGCAATAGCA GCAATAAATG AGGGACAAAA TAAGCCCGAA
    ATAGAAGTCA CCTGGGCAAA GCAAGGTAGG ACTGAAAGGC TGTGCTCTCA AAACCCACCA
    GGCTTGAAAC GCCATCAACG GGAAATAACT CGTACTACTC TTCAGTCAGA TCAAGAGGAA
    ATTGACTATG ATGATACCAT CTCAGTTGAA ATGAAGAAGG AAGATTTTGA CATTTATGAT
    GAGGATGAAA ATCAGAGCCC CCGCAGCTTT CAAAAGAAAA CACGACACTA TTTTATTGCT
    GCAGTGGAGA GGCTCTGGGA TTATGGGATG AGTAGCTCCC CACATGTTCT AAGAAACAGG
    GCTCAGAGTG GCAGTGTCCC TCAGTTCAAG AAAGTTGTTT TCCAGGAATT TACTGATGGC
    TCCTTTACTC AGCCCTTATA CCGTGGAGAA CTAAATGAAC ATTTGGGACT CCTGGGGCCA
    TATATAAGAG CAGAAGTTGA AGATAATATC ATGGTAACTT TCAAAAATCA GGCCTCTCGT
    CCCTATTCCT TCTATTCTAG CCTTATTTCT TATGAGGAAG ATCAGAGGCA AGGAGCAGAA
    CCTAGGAAAA ACTTTGTCAA GCCTAATGAA ACCAAAACTT ACTTTTGGAA AGTGCAACAT
    CATATGGCGC CCACTAAAGA TGAGTTTGAC TGCAAAGCCT GGGCTTATTT CTCTGATGTT
    GACCTGGAAA AAGATGTGCA CTCAGGCCTG ATTGGACCCC TTCTGGTCTG CCACACTAAC
    ACACTGAACC CTGCTCATGG GAGACAAGTG ACAGTACAGG AATTTGCTCT GTTTTTCACC
    ATCTTTGATG AGACCAAAAG CTGGTACTTC ACTGAAAACA TGGAAAGAAA CTGCAGGGCT
    CCCTGCAATA TCCAGATGGA AGATCCCACT TTTAAAGAGA ATTATCGCTT CCATGCAATC
    AATGGCTACA TAATGGATAC ACTACCTGGC TTAGTAATGG CTCAGGATCA AAGGATTCGA
    TGGTATCTGC TCAGCATGGG CAGCAATGAA AACATCCATT CTATTCATTT CAGTGGACAT
    GTGTTCACTG TACGAAAAAA AGAGGAGTAT AAAATGGCAC TGTACAATCT CTATCCAGGT
    GTTTTTGAGA CAGTGGAAAT GTTACCATCC AAAGCTGGAA TTTGGCGGGT GGAATGCCTT
    ATTGGCGAGC ATCTACATGC TGGGATGAGC ACACTTTTTC TGGTGTACAG CAATAAGTGT
    CAGACTCCCC TGGGAATGGC TTCTGGACAC ATTAGAGATT TTCAGATTAC AGCTTCAGGA
    CAATATGGAC AGTGGGCCCC AAAGCTGGCC AGACTTCATT ATTCCGGATC AATCAATGCC
    TGGAGCACCA AGGAGCCCTT TTCTTGGATC AAGGTGGATC TGTTGGCACC AATGATTATT
    CACGGCATCA AGACCCAGGG TGCCCGTCAG AAGTTCTCCA GCCTCTACAT CTCTCAGTTT
    ATCATCATGT ATAGTCTTGA TGGGAAGAAG TGGCAGACTT ATCGAGGAAA TTCCACTGGA
    ACCTTAATGG TCTTCTTTGG CAATGTGGAT TCATCTGGGA TAAAACACAA TATTTTTAAC
    CCTCCAATTA TTGCTCGATA CATCCGTTTG CACCCAACTC ATTATAGCAT TCGCAGCACT
    CTTCGCATGG AGTTGATGGG CTGTGATTTA AATAGTTGCA GCATGCCATT GGGAATGGAG
    AGTAAAGCAA TATCAGATGC ACAGATTACT GCTTCATCCT ACTTTACCAA TATGTTTGCC
    ACCTGGTCTC CTTCAAAAGC TCGACTTCAC CTCCAAGGGA GGAGTAATGC CTGGAGACCT
    CAGGTGAATA ATCCAAAAGA GTGGCTGCAA GTGGACTTCC AGAAGACAAT GAAAGTCACA
    GGAATAACTA CTCAGGGAGT AAAATCTCTG CTTACCAGCA TGTATGTGAA GGAGTTCCTC
    ATCTCCAGCA GTCAAGATGG CCATCACTGG ACTCTCTTTT TTCAGAATGG CAAAGTAAAG
    GTTTTTCAGG GAAATCAAGA CTCCTTCACA CCTGTGGTGA ACTCTCTAGA CCCACCGTTA
    CTGACTCGCT ACCTTCGAAT TCACCCCCAG AGTTGGGTGC ACCAGATTGC CCTGAGGATG
    GAGGTTCTGG GCTGCGAGGC ACAGGACCTC TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc91296
    Clone ID Related Accession (Same CDS sequence) XM_016944624.1
    Accession Version XM_016944624.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6951bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product coagulation factor VIII isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036902.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGCACCGAG ATGCGCGTGC GCAGAAAGCA TCCCGGGGAT TTCCTCCTAG AGTGCCAAAA 
    TCTTTTCCAT TCAACACCTC AGTCGTGTAC AAAAAGACTG TGTTTGTAGA ATTCACGGAT
    CACCTTTTCA ACATCGCTAA GCCAAGGCCA CCCTGGATGG GTCTGCTAGG TCCTACCATC
    CAGGCTGAGG TTTATGATAC AGTGGTCATT ACACTTAAGA ACATGGCTTC CCATCCTGTC
    AGTCTTCATG CTGTTGGTGT ATCCTACTGG AAAGCTTCTG AGGGAGCTGA ATATGATGAT
    CAGACCAGTC AAAGGGAGAA AGAAGATGAT AAAGTCTTTC CTGGTGGAAG CCATACATAT
    GTCTGGCAGG TCCTGAAAGA GAATGGTCCA ATGGCCTCTG ACCCACTGTG CCTTACCTAC
    TCATATCTTT CTCATGTGGA CCTGGTCAAA GACTTGAATT CAGGCCTCAT TGGAGCCCTA
    CTAGTATGTA GAGAAGGGAG TCTGGCCAAG GAAAAGACAC AGACCTTGCA CAAATTTATA
    CTACTTTTTG CTGTATTTGA TGAAGGGAAA AGTTGGCACT CAGAAACAAA GAACTCCTTG
    ATGCAGGATA GGGATGCTGC ATCTGCTCGG GCCTGGCCTA AAATGCACAC AGTCAATGGT
    TATGTAAACA GGTCTCTGCC AGGTCTGATT GGATGCCACA GGAAATCAGT CTATTGGCAT
    GTGATTGGAA TGGGCACCAC TCCTGAAGTG CACTCAATAT TCCTCGAAGG TCACACATTT
    CTTGTGAGGA ACCATCGCCA GGCGTCCTTG GAAATATCGC CAATAACTTT CCTTACTGCT
    CAAACACTCT TGATGGACCT TGGACAGTTT CTACTGTTTT GTCATATCTC TTCCCACCAA
    CATGATGGCA TGGAAGCTTA TGTCAAAGTA GACAGCTGCC CAGAGGAACC CCAACTACGA
    ATGAAAAATA ATGAAGAAGC GGAAGACTAT GATGATGATC TTACTGATTC TGAAATGGAT
    GTGGTCAGGT TTGATGATGA CAACTCTCCT TCCTTTATCC AAATTCGCTC AGTTGCCAAG
    AAGCATCCTA AAACTTGGGT ACATTACATT GCTGCTGAAG AGGAGGACTG GGACTATGCT
    CCCTTAGTCC TCGCCCCCGA TGACAGAAGT TATAAAAGTC AATATTTGAA CAATGGCCCT
    CAGCGGATTG GTAGGAAGTA CAAAAAAGTC CGATTTATGG CATACACAGA TGAAACCTTT
    AAGACTCGTG AAGCTATTCA GCATGAATCA GGAATCTTGG GACCTTTACT TTATGGGGAA
    GTTGGAGACA CACTGTTGAT TATATTTAAG AATCAAGCAA GCAGACCATA TAACATCTAC
    CCTCACGGAA TCACTGATGT CCGTCCTTTG TATTCAAGGA GATTACCAAA AGGTGTAAAA
    CATTTGAAGG ATTTTCCAAT TCTGCCAGGA GAAATATTCA AATATAAATG GACAGTGACT
    GTAGAAGATG GGCCAACTAA ATCAGATCCT CGGTGCCTGA CCCGCTATTA CTCTAGTTTC
    GTTAATATGG AGAGAGATCT AGCTTCAGGA CTCATTGGCC CTCTCCTCAT CTGCTACAAA
    GAATCTGTAG ATCAAAGAGG AAACCAGATA ATGTCAGACA AGAGGAATGT CATCCTGTTT
    TCTGTATTTG ATGAGAACCG AAGCTGGTAC CTCACAGAGA ATATACAACG CTTTCTCCCC
    AATCCAGCTG GAGTGCAGCT TGAGGATCCA GAGTTCCAAG CCTCCAACAT CATGCACAGC
    ATCAATGGCT ATGTTTTTGA TAGTTTGCAG TTGTCAGTTT GTTTGCATGA GGTGGCATAC
    TGGTACATTC TAAGCATTGG AGCACAGACT GACTTCCTTT CTGTCTTCTT CTCTGGATAT
    ACCTTCAAAC ACAAAATGGT CTATGAAGAT ACACTCACCC TATTCCCATT CTCAGGAGAA
    ACTGTCTTCA TGTCGATGGA AAACCCAGGC CTATGGATTC TGGGGTGCCA CAACTCAGAC
    TTTCGGAACA GAGGCATGAC CGCCTTACTG AAGGTTTCTA GTTGTGACAA GAACACTGGT
    GATTATTACG AGGACAGTTA TGAAGATATT TCAGCATACT TGCTGAGTAA AAACAATGCC
    ATTGAACCAA GAAGCTTCTC CCAGAATTCA AGACACCCTA GCACTAGGCA AAAGCAATTT
    AATGCCACCA CAATTCCAGA AAATGACATA GAGAAGACTG ACCCTTGGTT TGCACACAGA
    ACACCTATGC CTAAAGTACA AAATGTCTCC TCTAGTGATT TGTTGATGCT CTTGCGACAG
    AGTCCTACTC CACATGGGCT ATCCTTATCT GATCTCCAAG AAGCCAAATA TGAGACTTTT
    TCTGATGATC CATCACCTGG AGCAATAGAC AGTAATAACA GCCTATCTGA AATGACACAC
    TTCAGGCCAC AGTTCCATCA CAGTGGGGAC ATGGTATTTA CCCCTGAGTC AGGCCTCCAA
    TTGAGATTAA ATGAGAAACT GGGGACAACT GCAGCAACAG AGTTGAAGAA ACTTGATTTC
    AAAGTTTCTA GTACATCAAA TAATCTGATT TCAACAATTC CATCAGACAA TTTGGCAGCA
    GGTACTGATA ATACAAGTTC CTTAGGACCC CCAAGTATGC CAGTTCATTA TGATAGTCAA
    TTAGATACCA CTCTATTTGG CAAAAAGTCA TCTCCCCTTA CTGAGTCTGG TAGACCTCTG
    AGCTTGAGTG AAGAAAATAA TGATTCAAAG TTGTTAGAAT CAGGTTTAAT GAATAGCCAA
    GAAAGTTCAT GGGGAAAAAA TGTATCGTCA ACAGAGAGTG GTAGGTTATT TAAAGGGAAA
    AGAGCTCATG GACCTGCTTT GTTGACTAAA GATAATGCCT TATTCAAAGT TAGCATCTCT
    TTGTTAAAGA CAAACAAAAC TTCCAATAAT TCAGCAACTA ATAGAAAGAC TCACATTGAT
    GGCCCATCAT TATTAATTCA GAGTAGTCCA TCAGTCTGGC AAAATATATT AGAAAGTGAC
    ACTGAGTTTA AAAAAGTGAC ACCTTTGATT CATGACAGAA TGCTTATGGA CAAAAATGCT
    ACAGCTTTGA GGCTAAATCA TATGTCAAAT AAAACTACTT CATCAAAAAA CATGGAAATG
    GTCCGACAGA AAAAAGAGGG CCCCATTCCA CCAGATGCAG AAAATCCAGA TATGTCATTC
    TTTAAGATGC TATTCTTGCC AGAATCAGCA AGTTGGATAC AAAGGACTCA TGGAAAGAAC
    TCTCTGAACT CTGGGCAAGG CCCCAGTCCA AAGCAATTAG TATCCTTAGG ACCAGAAAAA
    TCTGTGGAAG GTCAGAATTT CTTGTCTGAG AAAAACAAAG TGGTAGTAGG AAAGGGTGAA
    TTTACAAAGG ACGTAGGACT CAAAGAGATG GTTTTTCCAA GCAGCAGAAA CCTATTTCTT
    ACTAACTTGG ATAATTTACA TGAAAATAAT ACACACAATC AAGAAAAAAA AATTCAGGAA
    GAAATAGAAA GGAAGGAAAC ATTAATCCAA GAGAATGTAG TTTTGCCTCA GATACATACA
    GTGACTGGCA CTAAGAATTT CATGAAGAAC CTTTTCTTAC TGAGCACTAG GCAAAATGTA
    GAAGGTTCAT ATGAGGGGGC ATATGCTCCA GTACTTCAAG ATTTTAGGTC ATTAAATGAT
    TCAACAAATA GAACAAAGAA ACACACGGCT CATTTCTCAA AAAAAGGGGA AGAAGAAAAC
    TTGGCAGGCT TGGGAAATCA AACCAAGCAA ATTGTAGAGA AATATGCATG CACCACAAGG
    ATATCTCCTA ATACAAGCCA GCAGAATTTT GTCACGCAAC GTAGTAAGAG AGCTTTGAAA
    CAATTCAGAC TCCCACTAGA AGAAACAGAA CTTGAAAAAA GGATAATTGT GGATGACACC
    TCAACCCAGT GGTCCAAAAA CATGAAACAT TTGACCCCGA GCACCCTCAC ACAGATAGAC
    TACAATGAGA AGGAGAAAGG GGCCATTACT CAGTCTCCCT TATCAGATTG CCTTACGAGG
    AGTCATAGCA TCCCTCAAGC AAATAGATCT CCATTACCCA TTGCAGAGGT ATCATCATTT
    CCATCTATTA GACCTATATA TCTGACCAGG GTCCTATTCC AAGACAACTC TTCTCATCTT
    CCAGCAGCAT CTTATAGAAA GAAAGATTCT GGGGTCCAAG AAAGCAGTCA TTTCTTACAA
    GGAGCCAAAA AAAATAACCT TTCTTTAGCC ATTCTAACCT TGGAGATGAC TGGTGATCAA
    AGAGAGGTTG GCTCCCTGGG GACAAGTGCC ACAAATTCAG TCACTTACAA GAAAGTTGAG
    AACACTGTTC TCCCGAAACC AGGCTTGCCC AAAACATCTG GCAAAGTTGA ATTGCTTCCA
    AAAGTTCACA TTTATCAGAA GGACCTATTC CCTACGGAAA CTAGCAATGG GTCTCCTGGC
    CATCTGGATC TCGTGGAAGG GAGCCTTCTT CAGGAAACAG AGGGAGCGAT TAAGTGGAAT
    GAAGCAAACA GACCTGGAAA AATTCCCTTT CTGAGAGTAG CAACAGAAAG CTCTGCAAAG
    ACTCCCTCCA AGCTATTGGA TCCTCTTGCT TGGGATAACC ACTATGGTAC TCAGATACCA
    AAAGAAGAGT GGAAATCCCA AGAGAAGTCA CCAGAAAAAA CAGCTTTTAA GAAAAAGGAT
    ACCATTTTGT CCCTGAACGC TTGTGAAAGC AATCATGCAA TAGCAGCAAT AAATGAGGGA
    CAAAATAAGC CCGAAATAGA AGTCACCTGG GCAAAGCAAG GTAGGACTGA AAGGCTGTGC
    TCTCAAAACC CACCAGGCTT GAAACGCCAT CAACGGGAAA TAACTCGTAC TACTCTTCAG
    TCAGATCAAG AGGAAATTGA CTATGATGAT ACCATCTCAG TTGAAATGAA GAAGGAAGAT
    TTTGACATTT ATGATGAGGA TGAAAATCAG AGCCCCCGCA GCTTTCAAAA GAAAACACGA
    CACTATTTTA TTGCTGCAGT GGAGAGGCTC TGGGATTATG GGATGAGTAG CTCCCCACAT
    GTTCTAAGAA ACAGGGCTCA GAGTGGCAGT GTCCCTCAGT TCAAGAAAGT TGTTTTCCAG
    GAATTTACTG ATGGCTCCTT TACTCAGCCC TTATACCGTG GAGAACTAAA TGAACATTTG
    GGACTCCTGG GGCCATATAT AAGAGCAGAA GTTGAAGATA ATATCATGGT AACTTTCAAA
    AATCAGGCCT CTCGTCCCTA TTCCTTCTAT TCTAGCCTTA TTTCTTATGA GGAAGATCAG
    AGGCAAGGAG CAGAACCTAG GAAAAACTTT GTCAAGCCTA ATGAAACCAA AACTTACTTT
    TGGAAAGTGC AACATCATAT GGCGCCCACT AAAGATGAGT TTGACTGCAA AGCCTGGGCT
    TATTTCTCTG ATGTTGACCT GGAAAAAGAT GTGCACTCAG GCCTGATTGG ACCCCTTCTG
    GTCTGCCACA CTAACACACT GAACCCTGCT CATGGGAGAC AAGTGACAGT ACAGGAATTT
    GCTCTGTTTT TCACCATCTT TGATGAGACC AAAAGCTGGT ACTTCACTGA AAACATGGAA
    AGAAACTGCA GGGCTCCCTG CAATATCCAG ATGGAAGATC CCACTTTTAA AGAGAATTAT
    CGCTTCCATG CAATCAATGG CTACATAATG GATACACTAC CTGGCTTAGT AATGGCTCAG
    GATCAAAGGA TTCGATGGTA TCTGCTCAGC ATGGGCAGCA ATGAAAACAT CCATTCTATT
    CATTTCAGTG GACATGTGTT CACTGTACGA AAAAAAGAGG AGTATAAAAT GGCACTGTAC
    AATCTCTATC CAGGTGTTTT TGAGACAGTG GAAATGTTAC CATCCAAAGC TGGAATTTGG
    CGGGTGGAAT GCCTTATTGG CGAGCATCTA CATGCTGGGA TGAGCACACT TTTTCTGGTG
    TACAGCAATA AGTGTCAGAC TCCCCTGGGA ATGGCTTCTG GACACATTAG AGATTTTCAG
    ATTACAGCTT CAGGACAATA TGGACAGTGG GCCCCAAAGC TGGCCAGACT TCATTATTCC
    GGATCAATCA ATGCCTGGAG CACCAAGGAG CCCTTTTCTT GGATCAAGGT GGATCTGTTG
    GCACCAATGA TTATTCACGG CATCAAGACC CAGGGTGCCC GTCAGAAGTT CTCCAGCCTC
    TACATCTCTC AGTTTATCAT CATGTATAGT CTTGATGGGA AGAAGTGGCA GACTTATCGA
    GGAAATTCCA CTGGAACCTT AATGGTCTTC TTTGGCAATG TGGATTCATC TGGGATAAAA
    CACAATATTT TTAACCCTCC AATTATTGCT CGATACATCC GTTTGCACCC AACTCATTAT
    AGCATTCGCA GCACTCTTCG CATGGAGTTG ATGGGCTGTG ATTTAAATAG TTGCAGCATG
    CCATTGGGAA TGGAGAGTAA AGCAATATCA GATGCACAGA TTACTGCTTC ATCCTACTTT
    ACCAATATGT TTGCCACCTG GTCTCCTTCA AAAGCTCGAC TTCACCTCCA AGGGAGGAGT
    AATGCCTGGA GACCTCAGGT GAATAATCCA AAAGAGTGGC TGCAAGTGGA CTTCCAGAAG
    ACAATGAAAG TCACAGGAAT AACTACTCAG GGAGTAAAAT CTCTGCTTAC CAGCATGTAT
    GTGAAGGAGT TCCTCATCTC CAGCAGTCAA GATGGCCATC ACTGGACTCT CTTTTTTCAG
    AATGGCAAAG TAAAGGTTTT TCAGGGAAAT CAAGACTCCT TCACACCTGT GGTGAACTCT
    CTAGACCCAC CGTTACTGAC TCGCTACCTT CGAATTCACC CCCAGAGTTG GGTGCACCAG
    ATTGCCCTGA GGATGGAGGT TCTGGGCTGC GAGGCACAGG ACCTCTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016800113.1
    CDS118..7068
    Translation

    Target ORF information:

    RefSeq Version XM_016944624.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes coagulation factor VIII (F8), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016944624.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGCACCGAG ATGCGCGTGC GCAGAAAGCA TCCCGGGGAT TTCCTCCTAG AGTGCCAAAA 
    TCTTTTCCAT TCAACACCTC AGTCGTGTAC AAAAAGACTG TGTTTGTAGA ATTCACGGAT
    CACCTTTTCA ACATCGCTAA GCCAAGGCCA CCCTGGATGG GTCTGCTAGG TCCTACCATC
    CAGGCTGAGG TTTATGATAC AGTGGTCATT ACACTTAAGA ACATGGCTTC CCATCCTGTC
    AGTCTTCATG CTGTTGGTGT ATCCTACTGG AAAGCTTCTG AGGGAGCTGA ATATGATGAT
    CAGACCAGTC AAAGGGAGAA AGAAGATGAT AAAGTCTTTC CTGGTGGAAG CCATACATAT
    GTCTGGCAGG TCCTGAAAGA GAATGGTCCA ATGGCCTCTG ACCCACTGTG CCTTACCTAC
    TCATATCTTT CTCATGTGGA CCTGGTCAAA GACTTGAATT CAGGCCTCAT TGGAGCCCTA
    CTAGTATGTA GAGAAGGGAG TCTGGCCAAG GAAAAGACAC AGACCTTGCA CAAATTTATA
    CTACTTTTTG CTGTATTTGA TGAAGGGAAA AGTTGGCACT CAGAAACAAA GAACTCCTTG
    ATGCAGGATA GGGATGCTGC ATCTGCTCGG GCCTGGCCTA AAATGCACAC AGTCAATGGT
    TATGTAAACA GGTCTCTGCC AGGTCTGATT GGATGCCACA GGAAATCAGT CTATTGGCAT
    GTGATTGGAA TGGGCACCAC TCCTGAAGTG CACTCAATAT TCCTCGAAGG TCACACATTT
    CTTGTGAGGA ACCATCGCCA GGCGTCCTTG GAAATATCGC CAATAACTTT CCTTACTGCT
    CAAACACTCT TGATGGACCT TGGACAGTTT CTACTGTTTT GTCATATCTC TTCCCACCAA
    CATGATGGCA TGGAAGCTTA TGTCAAAGTA GACAGCTGCC CAGAGGAACC CCAACTACGA
    ATGAAAAATA ATGAAGAAGC GGAAGACTAT GATGATGATC TTACTGATTC TGAAATGGAT
    GTGGTCAGGT TTGATGATGA CAACTCTCCT TCCTTTATCC AAATTCGCTC AGTTGCCAAG
    AAGCATCCTA AAACTTGGGT ACATTACATT GCTGCTGAAG AGGAGGACTG GGACTATGCT
    CCCTTAGTCC TCGCCCCCGA TGACAGAAGT TATAAAAGTC AATATTTGAA CAATGGCCCT
    CAGCGGATTG GTAGGAAGTA CAAAAAAGTC CGATTTATGG CATACACAGA TGAAACCTTT
    AAGACTCGTG AAGCTATTCA GCATGAATCA GGAATCTTGG GACCTTTACT TTATGGGGAA
    GTTGGAGACA CACTGTTGAT TATATTTAAG AATCAAGCAA GCAGACCATA TAACATCTAC
    CCTCACGGAA TCACTGATGT CCGTCCTTTG TATTCAAGGA GATTACCAAA AGGTGTAAAA
    CATTTGAAGG ATTTTCCAAT TCTGCCAGGA GAAATATTCA AATATAAATG GACAGTGACT
    GTAGAAGATG GGCCAACTAA ATCAGATCCT CGGTGCCTGA CCCGCTATTA CTCTAGTTTC
    GTTAATATGG AGAGAGATCT AGCTTCAGGA CTCATTGGCC CTCTCCTCAT CTGCTACAAA
    GAATCTGTAG ATCAAAGAGG AAACCAGATA ATGTCAGACA AGAGGAATGT CATCCTGTTT
    TCTGTATTTG ATGAGAACCG AAGCTGGTAC CTCACAGAGA ATATACAACG CTTTCTCCCC
    AATCCAGCTG GAGTGCAGCT TGAGGATCCA GAGTTCCAAG CCTCCAACAT CATGCACAGC
    ATCAATGGCT ATGTTTTTGA TAGTTTGCAG TTGTCAGTTT GTTTGCATGA GGTGGCATAC
    TGGTACATTC TAAGCATTGG AGCACAGACT GACTTCCTTT CTGTCTTCTT CTCTGGATAT
    ACCTTCAAAC ACAAAATGGT CTATGAAGAT ACACTCACCC TATTCCCATT CTCAGGAGAA
    ACTGTCTTCA TGTCGATGGA AAACCCAGGC CTATGGATTC TGGGGTGCCA CAACTCAGAC
    TTTCGGAACA GAGGCATGAC CGCCTTACTG AAGGTTTCTA GTTGTGACAA GAACACTGGT
    GATTATTACG AGGACAGTTA TGAAGATATT TCAGCATACT TGCTGAGTAA AAACAATGCC
    ATTGAACCAA GAAGCTTCTC CCAGAATTCA AGACACCCTA GCACTAGGCA AAAGCAATTT
    AATGCCACCA CAATTCCAGA AAATGACATA GAGAAGACTG ACCCTTGGTT TGCACACAGA
    ACACCTATGC CTAAAGTACA AAATGTCTCC TCTAGTGATT TGTTGATGCT CTTGCGACAG
    AGTCCTACTC CACATGGGCT ATCCTTATCT GATCTCCAAG AAGCCAAATA TGAGACTTTT
    TCTGATGATC CATCACCTGG AGCAATAGAC AGTAATAACA GCCTATCTGA AATGACACAC
    TTCAGGCCAC AGTTCCATCA CAGTGGGGAC ATGGTATTTA CCCCTGAGTC AGGCCTCCAA
    TTGAGATTAA ATGAGAAACT GGGGACAACT GCAGCAACAG AGTTGAAGAA ACTTGATTTC
    AAAGTTTCTA GTACATCAAA TAATCTGATT TCAACAATTC CATCAGACAA TTTGGCAGCA
    GGTACTGATA ATACAAGTTC CTTAGGACCC CCAAGTATGC CAGTTCATTA TGATAGTCAA
    TTAGATACCA CTCTATTTGG CAAAAAGTCA TCTCCCCTTA CTGAGTCTGG TAGACCTCTG
    AGCTTGAGTG AAGAAAATAA TGATTCAAAG TTGTTAGAAT CAGGTTTAAT GAATAGCCAA
    GAAAGTTCAT GGGGAAAAAA TGTATCGTCA ACAGAGAGTG GTAGGTTATT TAAAGGGAAA
    AGAGCTCATG GACCTGCTTT GTTGACTAAA GATAATGCCT TATTCAAAGT TAGCATCTCT
    TTGTTAAAGA CAAACAAAAC TTCCAATAAT TCAGCAACTA ATAGAAAGAC TCACATTGAT
    GGCCCATCAT TATTAATTCA GAGTAGTCCA TCAGTCTGGC AAAATATATT AGAAAGTGAC
    ACTGAGTTTA AAAAAGTGAC ACCTTTGATT CATGACAGAA TGCTTATGGA CAAAAATGCT
    ACAGCTTTGA GGCTAAATCA TATGTCAAAT AAAACTACTT CATCAAAAAA CATGGAAATG
    GTCCGACAGA AAAAAGAGGG CCCCATTCCA CCAGATGCAG AAAATCCAGA TATGTCATTC
    TTTAAGATGC TATTCTTGCC AGAATCAGCA AGTTGGATAC AAAGGACTCA TGGAAAGAAC
    TCTCTGAACT CTGGGCAAGG CCCCAGTCCA AAGCAATTAG TATCCTTAGG ACCAGAAAAA
    TCTGTGGAAG GTCAGAATTT CTTGTCTGAG AAAAACAAAG TGGTAGTAGG AAAGGGTGAA
    TTTACAAAGG ACGTAGGACT CAAAGAGATG GTTTTTCCAA GCAGCAGAAA CCTATTTCTT
    ACTAACTTGG ATAATTTACA TGAAAATAAT ACACACAATC AAGAAAAAAA AATTCAGGAA
    GAAATAGAAA GGAAGGAAAC ATTAATCCAA GAGAATGTAG TTTTGCCTCA GATACATACA
    GTGACTGGCA CTAAGAATTT CATGAAGAAC CTTTTCTTAC TGAGCACTAG GCAAAATGTA
    GAAGGTTCAT ATGAGGGGGC ATATGCTCCA GTACTTCAAG ATTTTAGGTC ATTAAATGAT
    TCAACAAATA GAACAAAGAA ACACACGGCT CATTTCTCAA AAAAAGGGGA AGAAGAAAAC
    TTGGCAGGCT TGGGAAATCA AACCAAGCAA ATTGTAGAGA AATATGCATG CACCACAAGG
    ATATCTCCTA ATACAAGCCA GCAGAATTTT GTCACGCAAC GTAGTAAGAG AGCTTTGAAA
    CAATTCAGAC TCCCACTAGA AGAAACAGAA CTTGAAAAAA GGATAATTGT GGATGACACC
    TCAACCCAGT GGTCCAAAAA CATGAAACAT TTGACCCCGA GCACCCTCAC ACAGATAGAC
    TACAATGAGA AGGAGAAAGG GGCCATTACT CAGTCTCCCT TATCAGATTG CCTTACGAGG
    AGTCATAGCA TCCCTCAAGC AAATAGATCT CCATTACCCA TTGCAGAGGT ATCATCATTT
    CCATCTATTA GACCTATATA TCTGACCAGG GTCCTATTCC AAGACAACTC TTCTCATCTT
    CCAGCAGCAT CTTATAGAAA GAAAGATTCT GGGGTCCAAG AAAGCAGTCA TTTCTTACAA
    GGAGCCAAAA AAAATAACCT TTCTTTAGCC ATTCTAACCT TGGAGATGAC TGGTGATCAA
    AGAGAGGTTG GCTCCCTGGG GACAAGTGCC ACAAATTCAG TCACTTACAA GAAAGTTGAG
    AACACTGTTC TCCCGAAACC AGGCTTGCCC AAAACATCTG GCAAAGTTGA ATTGCTTCCA
    AAAGTTCACA TTTATCAGAA GGACCTATTC CCTACGGAAA CTAGCAATGG GTCTCCTGGC
    CATCTGGATC TCGTGGAAGG GAGCCTTCTT CAGGAAACAG AGGGAGCGAT TAAGTGGAAT
    GAAGCAAACA GACCTGGAAA AATTCCCTTT CTGAGAGTAG CAACAGAAAG CTCTGCAAAG
    ACTCCCTCCA AGCTATTGGA TCCTCTTGCT TGGGATAACC ACTATGGTAC TCAGATACCA
    AAAGAAGAGT GGAAATCCCA AGAGAAGTCA CCAGAAAAAA CAGCTTTTAA GAAAAAGGAT
    ACCATTTTGT CCCTGAACGC TTGTGAAAGC AATCATGCAA TAGCAGCAAT AAATGAGGGA
    CAAAATAAGC CCGAAATAGA AGTCACCTGG GCAAAGCAAG GTAGGACTGA AAGGCTGTGC
    TCTCAAAACC CACCAGGCTT GAAACGCCAT CAACGGGAAA TAACTCGTAC TACTCTTCAG
    TCAGATCAAG AGGAAATTGA CTATGATGAT ACCATCTCAG TTGAAATGAA GAAGGAAGAT
    TTTGACATTT ATGATGAGGA TGAAAATCAG AGCCCCCGCA GCTTTCAAAA GAAAACACGA
    CACTATTTTA TTGCTGCAGT GGAGAGGCTC TGGGATTATG GGATGAGTAG CTCCCCACAT
    GTTCTAAGAA ACAGGGCTCA GAGTGGCAGT GTCCCTCAGT TCAAGAAAGT TGTTTTCCAG
    GAATTTACTG ATGGCTCCTT TACTCAGCCC TTATACCGTG GAGAACTAAA TGAACATTTG
    GGACTCCTGG GGCCATATAT AAGAGCAGAA GTTGAAGATA ATATCATGGT AACTTTCAAA
    AATCAGGCCT CTCGTCCCTA TTCCTTCTAT TCTAGCCTTA TTTCTTATGA GGAAGATCAG
    AGGCAAGGAG CAGAACCTAG GAAAAACTTT GTCAAGCCTA ATGAAACCAA AACTTACTTT
    TGGAAAGTGC AACATCATAT GGCGCCCACT AAAGATGAGT TTGACTGCAA AGCCTGGGCT
    TATTTCTCTG ATGTTGACCT GGAAAAAGAT GTGCACTCAG GCCTGATTGG ACCCCTTCTG
    GTCTGCCACA CTAACACACT GAACCCTGCT CATGGGAGAC AAGTGACAGT ACAGGAATTT
    GCTCTGTTTT TCACCATCTT TGATGAGACC AAAAGCTGGT ACTTCACTGA AAACATGGAA
    AGAAACTGCA GGGCTCCCTG CAATATCCAG ATGGAAGATC CCACTTTTAA AGAGAATTAT
    CGCTTCCATG CAATCAATGG CTACATAATG GATACACTAC CTGGCTTAGT AATGGCTCAG
    GATCAAAGGA TTCGATGGTA TCTGCTCAGC ATGGGCAGCA ATGAAAACAT CCATTCTATT
    CATTTCAGTG GACATGTGTT CACTGTACGA AAAAAAGAGG AGTATAAAAT GGCACTGTAC
    AATCTCTATC CAGGTGTTTT TGAGACAGTG GAAATGTTAC CATCCAAAGC TGGAATTTGG
    CGGGTGGAAT GCCTTATTGG CGAGCATCTA CATGCTGGGA TGAGCACACT TTTTCTGGTG
    TACAGCAATA AGTGTCAGAC TCCCCTGGGA ATGGCTTCTG GACACATTAG AGATTTTCAG
    ATTACAGCTT CAGGACAATA TGGACAGTGG GCCCCAAAGC TGGCCAGACT TCATTATTCC
    GGATCAATCA ATGCCTGGAG CACCAAGGAG CCCTTTTCTT GGATCAAGGT GGATCTGTTG
    GCACCAATGA TTATTCACGG CATCAAGACC CAGGGTGCCC GTCAGAAGTT CTCCAGCCTC
    TACATCTCTC AGTTTATCAT CATGTATAGT CTTGATGGGA AGAAGTGGCA GACTTATCGA
    GGAAATTCCA CTGGAACCTT AATGGTCTTC TTTGGCAATG TGGATTCATC TGGGATAAAA
    CACAATATTT TTAACCCTCC AATTATTGCT CGATACATCC GTTTGCACCC AACTCATTAT
    AGCATTCGCA GCACTCTTCG CATGGAGTTG ATGGGCTGTG ATTTAAATAG TTGCAGCATG
    CCATTGGGAA TGGAGAGTAA AGCAATATCA GATGCACAGA TTACTGCTTC ATCCTACTTT
    ACCAATATGT TTGCCACCTG GTCTCCTTCA AAAGCTCGAC TTCACCTCCA AGGGAGGAGT
    AATGCCTGGA GACCTCAGGT GAATAATCCA AAAGAGTGGC TGCAAGTGGA CTTCCAGAAG
    ACAATGAAAG TCACAGGAAT AACTACTCAG GGAGTAAAAT CTCTGCTTAC CAGCATGTAT
    GTGAAGGAGT TCCTCATCTC CAGCAGTCAA GATGGCCATC ACTGGACTCT CTTTTTTCAG
    AATGGCAAAG TAAAGGTTTT TCAGGGAAAT CAAGACTCCT TCACACCTGT GGTGAACTCT
    CTAGACCCAC CGTTACTGAC TCGCTACCTT CGAATTCACC CCCAGAGTTG GGTGCACCAG
    ATTGCCCTGA GGATGGAGGT TCTGGGCTGC GAGGCACAGG ACCTCTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc91297
    Clone ID Related Accession (Same CDS sequence) XM_016944625.1 , XM_016944625.2
    Accession Version XM_016944625.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product coagulation factor VIII isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006491.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGCTTCCC ATCCTGTCAG TCTTCATGCT GTTGGTGTAT CCTACTGGAA AGCTTCTGAG 
    GGAGCTGAAT ATGATGATCA GACCAGTCAA AGGGAGAAAG AAGATGATAA AGTCTTTCCT
    GGTGGAAGCC ATACATATGT CTGGCAGGTC CTGAAAGAGA ATGGTCCAAT GGCCTCTGAC
    CCACTGTGCC TTACCTACTC ATATCTTTCT CATGTGGACC TGGTCAAAGA CTTGAATTCA
    GGCCTCATTG GAGCCCTACT AGTATGTAGA GAAGGGAGTC TGGCCAAGGA AAAGACACAG
    ACCTTGCACA AATTTATACT ACTTTTTGCT GTATTTGATG AAGGGAAAAG TTGGCACTCA
    GAAACAAAGA ACTCCTTGAT GCAGGATAGG GATGCTGCAT CTGCTCGGGC CTGGCCTAAA
    ATGCACACAG TCAATGGTTA TGTAAACAGG TCTCTGCCAG GTCTGATTGG ATGCCACAGG
    AAATCAGTCT ATTGGCATGT GATTGGAATG GGCACCACTC CTGAAGTGCA CTCAATATTC
    CTCGAAGGTC ACACATTTCT TGTGAGGAAC CATCGCCAGG CGTCCTTGGA AATATCGCCA
    ATAACTTTCC TTACTGCTCA AACACTCTTG ATGGACCTTG GACAGTTTCT ACTGTTTTGT
    CATATCTCTT CCCACCAACA TGATGGCATG GAAGCTTATG TCAAAGTAGA CAGCTGCCCA
    GAGGAACCCC AACTACGAAT GAAAAATAAT GAAGAAGCGG AAGACTATGA TGATGATCTT
    ACTGATTCTG AAATGGATGT GGTCAGGTTT GATGATGACA ACTCTCCTTC CTTTATCCAA
    ATTCGCTCAG TTGCCAAGAA GCATCCTAAA ACTTGGGTAC ATTACATTGC TGCTGAAGAG
    GAGGACTGGG ACTATGCTCC CTTAGTCCTC GCCCCCGATG ACAGAAGTTA TAAAAGTCAA
    TATTTGAACA ATGGCCCTCA GCGGATTGGT AGGAAGTACA AAAAAGTCCG ATTTATGGCA
    TACACAGATG AAACCTTTAA GACTCGTGAA GCTATTCAGC ATGAATCAGG AATCTTGGGA
    CCTTTACTTT ATGGGGAAGT TGGAGACACA CTGTTGATTA TATTTAAGAA TCAAGCAAGC
    AGACCATATA ACATCTACCC TCACGGAATC ACTGATGTCC GTCCTTTGTA TTCAAGGAGA
    TTACCAAAAG GTGTAAAACA TTTGAAGGAT TTTCCAATTC TGCCAGGAGA AATATTCAAA
    TATAAATGGA CAGTGACTGT AGAAGATGGG CCAACTAAAT CAGATCCTCG GTGCCTGACC
    CGCTATTACT CTAGTTTCGT TAATATGGAG AGAGATCTAG CTTCAGGACT CATTGGCCCT
    CTCCTCATCT GCTACAAAGA ATCTGTAGAT CAAAGAGGAA ACCAGATAAT GTCAGACAAG
    AGGAATGTCA TCCTGTTTTC TGTATTTGAT GAGAACCGAA GCTGGTACCT CACAGAGAAT
    ATACAACGCT TTCTCCCCAA TCCAGCTGGA GTGCAGCTTG AGGATCCAGA GTTCCAAGCC
    TCCAACATCA TGCACAGCAT CAATGGCTAT GTTTTTGATA GTTTGCAGTT GTCAGTTTGT
    TTGCATGAGG TGGCATACTG GTACATTCTA AGCATTGGAG CACAGACTGA CTTCCTTTCT
    GTCTTCTTCT CTGGATATAC CTTCAAACAC AAAATGGTCT ATGAAGATAC ACTCACCCTA
    TTCCCATTCT CAGGAGAAAC TGTCTTCATG TCGATGGAAA ACCCAGGCCT ATGGATTCTG
    GGGTGCCACA ACTCAGACTT TCGGAACAGA GGCATGACCG CCTTACTGAA GGTTTCTAGT
    TGTGACAAGA ACACTGGTGA TTATTACGAG GACAGTTATG AAGATATTTC AGCATACTTG
    CTGAGTAAAA ACAATGCCAT TGAACCAAGA AGCTTCTCCC AGAATTCAAG ACACCCTAGC
    ACTAGGCAAA AGCAATTTAA TGCCACCACA ATTCCAGAAA ATGACATAGA GAAGACTGAC
    CCTTGGTTTG CACACAGAAC ACCTATGCCT AAAGTACAAA ATGTCTCCTC TAGTGATTTG
    TTGATGCTCT TGCGACAGAG TCCTACTCCA CATGGGCTAT CCTTATCTGA TCTCCAAGAA
    GCCAAATATG AGACTTTTTC TGATGATCCA TCACCTGGAG CAATAGACAG TAATAACAGC
    CTATCTGAAA TGACACACTT CAGGCCACAG TTCCATCACA GTGGGGACAT GGTATTTACC
    CCTGAGTCAG GCCTCCAATT GAGATTAAAT GAGAAACTGG GGACAACTGC AGCAACAGAG
    TTGAAGAAAC TTGATTTCAA AGTTTCTAGT ACATCAAATA ATCTGATTTC AACAATTCCA
    TCAGACAATT TGGCAGCAGG TACTGATAAT ACAAGTTCCT TAGGACCCCC AAGTATGCCA
    GTTCATTATG ATAGTCAATT AGATACCACT CTATTTGGCA AAAAGTCATC TCCCCTTACT
    GAGTCTGGTA GACCTCTGAG CTTGAGTGAA GAAAATAATG ATTCAAAGTT GTTAGAATCA
    GGTTTAATGA ATAGCCAAGA AAGTTCATGG GGAAAAAATG TATCGTCAAC AGAGAGTGGT
    AGGTTATTTA AAGGGAAAAG AGCTCATGGA CCTGCTTTGT TGACTAAAGA TAATGCCTTA
    TTCAAAGTTA GCATCTCTTT GTTAAAGACA AACAAAACTT CCAATAATTC AGCAACTAAT
    AGAAAGACTC ACATTGATGG CCCATCATTA TTAATTCAGA GTAGTCCATC AGTCTGGCAA
    AATATATTAG AAAGTGACAC TGAGTTTAAA AAAGTGACAC CTTTGATTCA TGACAGAATG
    CTTATGGACA AAAATGCTAC AGCTTTGAGG CTAAATCATA TGTCAAATAA AACTACTTCA
    TCAAAAAACA TGGAAATGGT CCGACAGAAA AAAGAGGGCC CCATTCCACC AGATGCAGAA
    AATCCAGATA TGTCATTCTT TAAGATGCTA TTCTTGCCAG AATCAGCAAG TTGGATACAA
    AGGACTCATG GAAAGAACTC TCTGAACTCT GGGCAAGGCC CCAGTCCAAA GCAATTAGTA
    TCCTTAGGAC CAGAAAAATC TGTGGAAGGT CAGAATTTCT TGTCTGAGAA AAACAAAGTG
    GTAGTAGGAA AGGGTGAATT TACAAAGGAC GTAGGACTCA AAGAGATGGT TTTTCCAAGC
    AGCAGAAACC TATTTCTTAC TAACTTGGAT AATTTACATG AAAATAATAC ACACAATCAA
    GAAAAAAAAA TTCAGGAAGA AATAGAAAGG AAGGAAACAT TAATCCAAGA GAATGTAGTT
    TTGCCTCAGA TACATACAGT GACTGGCACT AAGAATTTCA TGAAGAACCT TTTCTTACTG
    AGCACTAGGC AAAATGTAGA AGGTTCATAT GAGGGGGCAT ATGCTCCAGT ACTTCAAGAT
    TTTAGGTCAT TAAATGATTC AACAAATAGA ACAAAGAAAC ACACGGCTCA TTTCTCAAAA
    AAAGGGGAAG AAGAAAACTT GGCAGGCTTG GGAAATCAAA CCAAGCAAAT TGTAGAGAAA
    TATGCATGCA CCACAAGGAT ATCTCCTAAT ACAAGCCAGC AGAATTTTGT CACGCAACGT
    AGTAAGAGAG CTTTGAAACA ATTCAGACTC CCACTAGAAG AAACAGAACT TGAAAAAAGG
    ATAATTGTGG ATGACACCTC AACCCAGTGG TCCAAAAACA TGAAACATTT GACCCCGAGC
    ACCCTCACAC AGATAGACTA CAATGAGAAG GAGAAAGGGG CCATTACTCA GTCTCCCTTA
    TCAGATTGCC TTACGAGGAG TCATAGCATC CCTCAAGCAA ATAGATCTCC ATTACCCATT
    GCAGAGGTAT CATCATTTCC ATCTATTAGA CCTATATATC TGACCAGGGT CCTATTCCAA
    GACAACTCTT CTCATCTTCC AGCAGCATCT TATAGAAAGA AAGATTCTGG GGTCCAAGAA
    AGCAGTCATT TCTTACAAGG AGCCAAAAAA AATAACCTTT CTTTAGCCAT TCTAACCTTG
    GAGATGACTG GTGATCAAAG AGAGGTTGGC TCCCTGGGGA CAAGTGCCAC AAATTCAGTC
    ACTTACAAGA AAGTTGAGAA CACTGTTCTC CCGAAACCAG GCTTGCCCAA AACATCTGGC
    AAAGTTGAAT TGCTTCCAAA AGTTCACATT TATCAGAAGG ACCTATTCCC TACGGAAACT
    AGCAATGGGT CTCCTGGCCA TCTGGATCTC GTGGAAGGGA GCCTTCTTCA GGAAACAGAG
    GGAGCGATTA AGTGGAATGA AGCAAACAGA CCTGGAAAAA TTCCCTTTCT GAGAGTAGCA
    ACAGAAAGCT CTGCAAAGAC TCCCTCCAAG CTATTGGATC CTCTTGCTTG GGATAACCAC
    TATGGTACTC AGATACCAAA AGAAGAGTGG AAATCCCAAG AGAAGTCACC AGAAAAAACA
    GCTTTTAAGA AAAAGGATAC CATTTTGTCC CTGAACGCTT GTGAAAGCAA TCATGCAATA
    GCAGCAATAA ATGAGGGACA AAATAAGCCC GAAATAGAAG TCACCTGGGC AAAGCAAGGT
    AGGACTGAAA GGCTGTGCTC TCAAAACCCA CCAGGCTTGA AACGCCATCA ACGGGAAATA
    ACTCGTACTA CTCTTCAGTC AGATCAAGAG GAAATTGACT ATGATGATAC CATCTCAGTT
    GAAATGAAGA AGGAAGATTT TGACATTTAT GATGAGGATG AAAATCAGAG CCCCCGCAGC
    TTTCAAAAGA AAACACGACA CTATTTTATT GCTGCAGTGG AGAGGCTCTG GGATTATGGG
    ATGAGTAGCT CCCCACATGT TCTAAGAAAC AGGGCTCAGA GTGGCAGTGT CCCTCAGTTC
    AAGAAAGTTG TTTTCCAGGA ATTTACTGAT GGCTCCTTTA CTCAGCCCTT ATACCGTGGA
    GAACTAAATG AACATTTGGG ACTCCTGGGG CCATATATAA GAGCAGAAGT TGAAGATAAT
    ATCATGGTAA CTTTCAAAAA TCAGGCCTCT CGTCCCTATT CCTTCTATTC TAGCCTTATT
    TCTTATGAGG AAGATCAGAG GCAAGGAGCA GAACCTAGGA AAAACTTTGT CAAGCCTAAT
    GAAACCAAAA CTTACTTTTG GAAAGTGCAA CATCATATGG CGCCCACTAA AGATGAGTTT
    GACTGCAAAG CCTGGGCTTA TTTCTCTGAT GTTGACCTGG AAAAAGATGT GCACTCAGGC
    CTGATTGGAC CCCTTCTGGT CTGCCACACT AACACACTGA ACCCTGCTCA TGGGAGACAA
    GTGACAGTAC AGGAATTTGC TCTGTTTTTC ACCATCTTTG ATGAGACCAA AAGCTGGTAC
    TTCACTGAAA ACATGGAAAG AAACTGCAGG GCTCCCTGCA ATATCCAGAT GGAAGATCCC
    ACTTTTAAAG AGAATTATCG CTTCCATGCA ATCAATGGCT ACATAATGGA TACACTACCT
    GGCTTAGTAA TGGCTCAGGA TCAAAGGATT CGATGGTATC TGCTCAGCAT GGGCAGCAAT
    GAAAACATCC ATTCTATTCA TTTCAGTGGA CATGTGTTCA CTGTACGAAA AAAAGAGGAG
    TATAAAATGG CACTGTACAA TCTCTATCCA GGTGTTTTTG AGACAGTGGA AATGTTACCA
    TCCAAAGCTG GAATTTGGCG GGTGGAATGC CTTATTGGCG AGCATCTACA TGCTGGGATG
    AGCACACTTT TTCTGGTGTA CAGCAATAAG TGTCAGACTC CCCTGGGAAT GGCTTCTGGA
    CACATTAGAG ATTTTCAGAT TACAGCTTCA GGACAATATG GACAGTGGGC CCCAAAGCTG
    GCCAGACTTC ATTATTCCGG ATCAATCAAT GCCTGGAGCA CCAAGGAGCC CTTTTCTTGG
    ATCAAGGTGG ATCTGTTGGC ACCAATGATT ATTCACGGCA TCAAGACCCA GGGTGCCCGT
    CAGAAGTTCT CCAGCCTCTA CATCTCTCAG TTTATCATCA TGTATAGTCT TGATGGGAAG
    AAGTGGCAGA CTTATCGAGG AAATTCCACT GGAACCTTAA TGGTCTTCTT TGGCAATGTG
    GATTCATCTG GGATAAAACA CAATATTTTT AACCCTCCAA TTATTGCTCG ATACATCCGT
    TTGCACCCAA CTCATTATAG CATTCGCAGC ACTCTTCGCA TGGAGTTGAT GGGCTGTGAT
    TTAAATAGTT GCAGCATGCC ATTGGGAATG GAGAGTAAAG CAATATCAGA TGCACAGATT
    ACTGCTTCAT CCTACTTTAC CAATATGTTT GCCACCTGGT CTCCTTCAAA AGCTCGACTT
    CACCTCCAAG GGAGGAGTAA TGCCTGGAGA CCTCAGGTGA ATAATCCAAA AGAGTGGCTG
    CAAGTGGACT TCCAGAAGAC AATGAAAGTC ACAGGAATAA CTACTCAGGG AGTAAAATCT
    CTGCTTACCA GCATGTATGT GAAGGAGTTC CTCATCTCCA GCAGTCAAGA TGGCCATCAC
    TGGACTCTCT TTTTTCAGAA TGGCAAAGTA AAGGTTTTTC AGGGAAATCA AGACTCCTTC
    ACACCTGTGG TGAACTCTCT AGACCCACCG TTACTGACTC GCTACCTTCG AATTCACCCC
    CAGAGTTGGG TGCACCAGAT TGCCCTGAGG ATGGAGGTTC TGGGCTGCGA GGCACAGGAC
    CTCTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016800114.1
    CDS138..6866
    Translation

    Target ORF information:

    RefSeq Version XM_016944625.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes coagulation factor VIII (F8), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016944625.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGCTTCCC ATCCTGTCAG TCTTCATGCT GTTGGTGTAT CCTACTGGAA AGCTTCTGAG 
    GGAGCTGAAT ATGATGATCA GACCAGTCAA AGGGAGAAAG AAGATGATAA AGTCTTTCCT
    GGTGGAAGCC ATACATATGT CTGGCAGGTC CTGAAAGAGA ATGGTCCAAT GGCCTCTGAC
    CCACTGTGCC TTACCTACTC ATATCTTTCT CATGTGGACC TGGTCAAAGA CTTGAATTCA
    GGCCTCATTG GAGCCCTACT AGTATGTAGA GAAGGGAGTC TGGCCAAGGA AAAGACACAG
    ACCTTGCACA AATTTATACT ACTTTTTGCT GTATTTGATG AAGGGAAAAG TTGGCACTCA
    GAAACAAAGA ACTCCTTGAT GCAGGATAGG GATGCTGCAT CTGCTCGGGC CTGGCCTAAA
    ATGCACACAG TCAATGGTTA TGTAAACAGG TCTCTGCCAG GTCTGATTGG ATGCCACAGG
    AAATCAGTCT ATTGGCATGT GATTGGAATG GGCACCACTC CTGAAGTGCA CTCAATATTC
    CTCGAAGGTC ACACATTTCT TGTGAGGAAC CATCGCCAGG CGTCCTTGGA AATATCGCCA
    ATAACTTTCC TTACTGCTCA AACACTCTTG ATGGACCTTG GACAGTTTCT ACTGTTTTGT
    CATATCTCTT CCCACCAACA TGATGGCATG GAAGCTTATG TCAAAGTAGA CAGCTGCCCA
    GAGGAACCCC AACTACGAAT GAAAAATAAT GAAGAAGCGG AAGACTATGA TGATGATCTT
    ACTGATTCTG AAATGGATGT GGTCAGGTTT GATGATGACA ACTCTCCTTC CTTTATCCAA
    ATTCGCTCAG TTGCCAAGAA GCATCCTAAA ACTTGGGTAC ATTACATTGC TGCTGAAGAG
    GAGGACTGGG ACTATGCTCC CTTAGTCCTC GCCCCCGATG ACAGAAGTTA TAAAAGTCAA
    TATTTGAACA ATGGCCCTCA GCGGATTGGT AGGAAGTACA AAAAAGTCCG ATTTATGGCA
    TACACAGATG AAACCTTTAA GACTCGTGAA GCTATTCAGC ATGAATCAGG AATCTTGGGA
    CCTTTACTTT ATGGGGAAGT TGGAGACACA CTGTTGATTA TATTTAAGAA TCAAGCAAGC
    AGACCATATA ACATCTACCC TCACGGAATC ACTGATGTCC GTCCTTTGTA TTCAAGGAGA
    TTACCAAAAG GTGTAAAACA TTTGAAGGAT TTTCCAATTC TGCCAGGAGA AATATTCAAA
    TATAAATGGA CAGTGACTGT AGAAGATGGG CCAACTAAAT CAGATCCTCG GTGCCTGACC
    CGCTATTACT CTAGTTTCGT TAATATGGAG AGAGATCTAG CTTCAGGACT CATTGGCCCT
    CTCCTCATCT GCTACAAAGA ATCTGTAGAT CAAAGAGGAA ACCAGATAAT GTCAGACAAG
    AGGAATGTCA TCCTGTTTTC TGTATTTGAT GAGAACCGAA GCTGGTACCT CACAGAGAAT
    ATACAACGCT TTCTCCCCAA TCCAGCTGGA GTGCAGCTTG AGGATCCAGA GTTCCAAGCC
    TCCAACATCA TGCACAGCAT CAATGGCTAT GTTTTTGATA GTTTGCAGTT GTCAGTTTGT
    TTGCATGAGG TGGCATACTG GTACATTCTA AGCATTGGAG CACAGACTGA CTTCCTTTCT
    GTCTTCTTCT CTGGATATAC CTTCAAACAC AAAATGGTCT ATGAAGATAC ACTCACCCTA
    TTCCCATTCT CAGGAGAAAC TGTCTTCATG TCGATGGAAA ACCCAGGCCT ATGGATTCTG
    GGGTGCCACA ACTCAGACTT TCGGAACAGA GGCATGACCG CCTTACTGAA GGTTTCTAGT
    TGTGACAAGA ACACTGGTGA TTATTACGAG GACAGTTATG AAGATATTTC AGCATACTTG
    CTGAGTAAAA ACAATGCCAT TGAACCAAGA AGCTTCTCCC AGAATTCAAG ACACCCTAGC
    ACTAGGCAAA AGCAATTTAA TGCCACCACA ATTCCAGAAA ATGACATAGA GAAGACTGAC
    CCTTGGTTTG CACACAGAAC ACCTATGCCT AAAGTACAAA ATGTCTCCTC TAGTGATTTG
    TTGATGCTCT TGCGACAGAG TCCTACTCCA CATGGGCTAT CCTTATCTGA TCTCCAAGAA
    GCCAAATATG AGACTTTTTC TGATGATCCA TCACCTGGAG CAATAGACAG TAATAACAGC
    CTATCTGAAA TGACACACTT CAGGCCACAG TTCCATCACA GTGGGGACAT GGTATTTACC
    CCTGAGTCAG GCCTCCAATT GAGATTAAAT GAGAAACTGG GGACAACTGC AGCAACAGAG
    TTGAAGAAAC TTGATTTCAA AGTTTCTAGT ACATCAAATA ATCTGATTTC AACAATTCCA
    TCAGACAATT TGGCAGCAGG TACTGATAAT ACAAGTTCCT TAGGACCCCC AAGTATGCCA
    GTTCATTATG ATAGTCAATT AGATACCACT CTATTTGGCA AAAAGTCATC TCCCCTTACT
    GAGTCTGGTA GACCTCTGAG CTTGAGTGAA GAAAATAATG ATTCAAAGTT GTTAGAATCA
    GGTTTAATGA ATAGCCAAGA AAGTTCATGG GGAAAAAATG TATCGTCAAC AGAGAGTGGT
    AGGTTATTTA AAGGGAAAAG AGCTCATGGA CCTGCTTTGT TGACTAAAGA TAATGCCTTA
    TTCAAAGTTA GCATCTCTTT GTTAAAGACA AACAAAACTT CCAATAATTC AGCAACTAAT
    AGAAAGACTC ACATTGATGG CCCATCATTA TTAATTCAGA GTAGTCCATC AGTCTGGCAA
    AATATATTAG AAAGTGACAC TGAGTTTAAA AAAGTGACAC CTTTGATTCA TGACAGAATG
    CTTATGGACA AAAATGCTAC AGCTTTGAGG CTAAATCATA TGTCAAATAA AACTACTTCA
    TCAAAAAACA TGGAAATGGT CCGACAGAAA AAAGAGGGCC CCATTCCACC AGATGCAGAA
    AATCCAGATA TGTCATTCTT TAAGATGCTA TTCTTGCCAG AATCAGCAAG TTGGATACAA
    AGGACTCATG GAAAGAACTC TCTGAACTCT GGGCAAGGCC CCAGTCCAAA GCAATTAGTA
    TCCTTAGGAC CAGAAAAATC TGTGGAAGGT CAGAATTTCT TGTCTGAGAA AAACAAAGTG
    GTAGTAGGAA AGGGTGAATT TACAAAGGAC GTAGGACTCA AAGAGATGGT TTTTCCAAGC
    AGCAGAAACC TATTTCTTAC TAACTTGGAT AATTTACATG AAAATAATAC ACACAATCAA
    GAAAAAAAAA TTCAGGAAGA AATAGAAAGG AAGGAAACAT TAATCCAAGA GAATGTAGTT
    TTGCCTCAGA TACATACAGT GACTGGCACT AAGAATTTCA TGAAGAACCT TTTCTTACTG
    AGCACTAGGC AAAATGTAGA AGGTTCATAT GAGGGGGCAT ATGCTCCAGT ACTTCAAGAT
    TTTAGGTCAT TAAATGATTC AACAAATAGA ACAAAGAAAC ACACGGCTCA TTTCTCAAAA
    AAAGGGGAAG AAGAAAACTT GGCAGGCTTG GGAAATCAAA CCAAGCAAAT TGTAGAGAAA
    TATGCATGCA CCACAAGGAT ATCTCCTAAT ACAAGCCAGC AGAATTTTGT CACGCAACGT
    AGTAAGAGAG CTTTGAAACA ATTCAGACTC CCACTAGAAG AAACAGAACT TGAAAAAAGG
    ATAATTGTGG ATGACACCTC AACCCAGTGG TCCAAAAACA TGAAACATTT GACCCCGAGC
    ACCCTCACAC AGATAGACTA CAATGAGAAG GAGAAAGGGG CCATTACTCA GTCTCCCTTA
    TCAGATTGCC TTACGAGGAG TCATAGCATC CCTCAAGCAA ATAGATCTCC ATTACCCATT
    GCAGAGGTAT CATCATTTCC ATCTATTAGA CCTATATATC TGACCAGGGT CCTATTCCAA
    GACAACTCTT CTCATCTTCC AGCAGCATCT TATAGAAAGA AAGATTCTGG GGTCCAAGAA
    AGCAGTCATT TCTTACAAGG AGCCAAAAAA AATAACCTTT CTTTAGCCAT TCTAACCTTG
    GAGATGACTG GTGATCAAAG AGAGGTTGGC TCCCTGGGGA CAAGTGCCAC AAATTCAGTC
    ACTTACAAGA AAGTTGAGAA CACTGTTCTC CCGAAACCAG GCTTGCCCAA AACATCTGGC
    AAAGTTGAAT TGCTTCCAAA AGTTCACATT TATCAGAAGG ACCTATTCCC TACGGAAACT
    AGCAATGGGT CTCCTGGCCA TCTGGATCTC GTGGAAGGGA GCCTTCTTCA GGAAACAGAG
    GGAGCGATTA AGTGGAATGA AGCAAACAGA CCTGGAAAAA TTCCCTTTCT GAGAGTAGCA
    ACAGAAAGCT CTGCAAAGAC TCCCTCCAAG CTATTGGATC CTCTTGCTTG GGATAACCAC
    TATGGTACTC AGATACCAAA AGAAGAGTGG AAATCCCAAG AGAAGTCACC AGAAAAAACA
    GCTTTTAAGA AAAAGGATAC CATTTTGTCC CTGAACGCTT GTGAAAGCAA TCATGCAATA
    GCAGCAATAA ATGAGGGACA AAATAAGCCC GAAATAGAAG TCACCTGGGC AAAGCAAGGT
    AGGACTGAAA GGCTGTGCTC TCAAAACCCA CCAGGCTTGA AACGCCATCA ACGGGAAATA
    ACTCGTACTA CTCTTCAGTC AGATCAAGAG GAAATTGACT ATGATGATAC CATCTCAGTT
    GAAATGAAGA AGGAAGATTT TGACATTTAT GATGAGGATG AAAATCAGAG CCCCCGCAGC
    TTTCAAAAGA AAACACGACA CTATTTTATT GCTGCAGTGG AGAGGCTCTG GGATTATGGG
    ATGAGTAGCT CCCCACATGT TCTAAGAAAC AGGGCTCAGA GTGGCAGTGT CCCTCAGTTC
    AAGAAAGTTG TTTTCCAGGA ATTTACTGAT GGCTCCTTTA CTCAGCCCTT ATACCGTGGA
    GAACTAAATG AACATTTGGG ACTCCTGGGG CCATATATAA GAGCAGAAGT TGAAGATAAT
    ATCATGGTAA CTTTCAAAAA TCAGGCCTCT CGTCCCTATT CCTTCTATTC TAGCCTTATT
    TCTTATGAGG AAGATCAGAG GCAAGGAGCA GAACCTAGGA AAAACTTTGT CAAGCCTAAT
    GAAACCAAAA CTTACTTTTG GAAAGTGCAA CATCATATGG CGCCCACTAA AGATGAGTTT
    GACTGCAAAG CCTGGGCTTA TTTCTCTGAT GTTGACCTGG AAAAAGATGT GCACTCAGGC
    CTGATTGGAC CCCTTCTGGT CTGCCACACT AACACACTGA ACCCTGCTCA TGGGAGACAA
    GTGACAGTAC AGGAATTTGC TCTGTTTTTC ACCATCTTTG ATGAGACCAA AAGCTGGTAC
    TTCACTGAAA ACATGGAAAG AAACTGCAGG GCTCCCTGCA ATATCCAGAT GGAAGATCCC
    ACTTTTAAAG AGAATTATCG CTTCCATGCA ATCAATGGCT ACATAATGGA TACACTACCT
    GGCTTAGTAA TGGCTCAGGA TCAAAGGATT CGATGGTATC TGCTCAGCAT GGGCAGCAAT
    GAAAACATCC ATTCTATTCA TTTCAGTGGA CATGTGTTCA CTGTACGAAA AAAAGAGGAG
    TATAAAATGG CACTGTACAA TCTCTATCCA GGTGTTTTTG AGACAGTGGA AATGTTACCA
    TCCAAAGCTG GAATTTGGCG GGTGGAATGC CTTATTGGCG AGCATCTACA TGCTGGGATG
    AGCACACTTT TTCTGGTGTA CAGCAATAAG TGTCAGACTC CCCTGGGAAT GGCTTCTGGA
    CACATTAGAG ATTTTCAGAT TACAGCTTCA GGACAATATG GACAGTGGGC CCCAAAGCTG
    GCCAGACTTC ATTATTCCGG ATCAATCAAT GCCTGGAGCA CCAAGGAGCC CTTTTCTTGG
    ATCAAGGTGG ATCTGTTGGC ACCAATGATT ATTCACGGCA TCAAGACCCA GGGTGCCCGT
    CAGAAGTTCT CCAGCCTCTA CATCTCTCAG TTTATCATCA TGTATAGTCT TGATGGGAAG
    AAGTGGCAGA CTTATCGAGG AAATTCCACT GGAACCTTAA TGGTCTTCTT TGGCAATGTG
    GATTCATCTG GGATAAAACA CAATATTTTT AACCCTCCAA TTATTGCTCG ATACATCCGT
    TTGCACCCAA CTCATTATAG CATTCGCAGC ACTCTTCGCA TGGAGTTGAT GGGCTGTGAT
    TTAAATAGTT GCAGCATGCC ATTGGGAATG GAGAGTAAAG CAATATCAGA TGCACAGATT
    ACTGCTTCAT CCTACTTTAC CAATATGTTT GCCACCTGGT CTCCTTCAAA AGCTCGACTT
    CACCTCCAAG GGAGGAGTAA TGCCTGGAGA CCTCAGGTGA ATAATCCAAA AGAGTGGCTG
    CAAGTGGACT TCCAGAAGAC AATGAAAGTC ACAGGAATAA CTACTCAGGG AGTAAAATCT
    CTGCTTACCA GCATGTATGT GAAGGAGTTC CTCATCTCCA GCAGTCAAGA TGGCCATCAC
    TGGACTCTCT TTTTTCAGAA TGGCAAAGTA AAGGTTTTTC AGGGAAATCA AGACTCCTTC
    ACACCTGTGG TGAACTCTCT AGACCCACCG TTACTGACTC GCTACCTTCG AATTCACCCC
    CAGAGTTGGG TGCACCAGAT TGCCCTGAGG ATGGAGGTTC TGGGCTGCGA GGCACAGGAC
    CTCTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc91297
    Clone ID Related Accession (Same CDS sequence) XM_016944625.1 , XM_016944625.2
    Accession Version XM_016944625.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product coagulation factor VIII isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036902.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016944625.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGCTTCCC ATCCTGTCAG TCTTCATGCT GTTGGTGTAT CCTACTGGAA AGCTTCTGAG 
    GGAGCTGAAT ATGATGATCA GACCAGTCAA AGGGAGAAAG AAGATGATAA AGTCTTTCCT
    GGTGGAAGCC ATACATATGT CTGGCAGGTC CTGAAAGAGA ATGGTCCAAT GGCCTCTGAC
    CCACTGTGCC TTACCTACTC ATATCTTTCT CATGTGGACC TGGTCAAAGA CTTGAATTCA
    GGCCTCATTG GAGCCCTACT AGTATGTAGA GAAGGGAGTC TGGCCAAGGA AAAGACACAG
    ACCTTGCACA AATTTATACT ACTTTTTGCT GTATTTGATG AAGGGAAAAG TTGGCACTCA
    GAAACAAAGA ACTCCTTGAT GCAGGATAGG GATGCTGCAT CTGCTCGGGC CTGGCCTAAA
    ATGCACACAG TCAATGGTTA TGTAAACAGG TCTCTGCCAG GTCTGATTGG ATGCCACAGG
    AAATCAGTCT ATTGGCATGT GATTGGAATG GGCACCACTC CTGAAGTGCA CTCAATATTC
    CTCGAAGGTC ACACATTTCT TGTGAGGAAC CATCGCCAGG CGTCCTTGGA AATATCGCCA
    ATAACTTTCC TTACTGCTCA AACACTCTTG ATGGACCTTG GACAGTTTCT ACTGTTTTGT
    CATATCTCTT CCCACCAACA TGATGGCATG GAAGCTTATG TCAAAGTAGA CAGCTGCCCA
    GAGGAACCCC AACTACGAAT GAAAAATAAT GAAGAAGCGG AAGACTATGA TGATGATCTT
    ACTGATTCTG AAATGGATGT GGTCAGGTTT GATGATGACA ACTCTCCTTC CTTTATCCAA
    ATTCGCTCAG TTGCCAAGAA GCATCCTAAA ACTTGGGTAC ATTACATTGC TGCTGAAGAG
    GAGGACTGGG ACTATGCTCC CTTAGTCCTC GCCCCCGATG ACAGAAGTTA TAAAAGTCAA
    TATTTGAACA ATGGCCCTCA GCGGATTGGT AGGAAGTACA AAAAAGTCCG ATTTATGGCA
    TACACAGATG AAACCTTTAA GACTCGTGAA GCTATTCAGC ATGAATCAGG AATCTTGGGA
    CCTTTACTTT ATGGGGAAGT TGGAGACACA CTGTTGATTA TATTTAAGAA TCAAGCAAGC
    AGACCATATA ACATCTACCC TCACGGAATC ACTGATGTCC GTCCTTTGTA TTCAAGGAGA
    TTACCAAAAG GTGTAAAACA TTTGAAGGAT TTTCCAATTC TGCCAGGAGA AATATTCAAA
    TATAAATGGA CAGTGACTGT AGAAGATGGG CCAACTAAAT CAGATCCTCG GTGCCTGACC
    CGCTATTACT CTAGTTTCGT TAATATGGAG AGAGATCTAG CTTCAGGACT CATTGGCCCT
    CTCCTCATCT GCTACAAAGA ATCTGTAGAT CAAAGAGGAA ACCAGATAAT GTCAGACAAG
    AGGAATGTCA TCCTGTTTTC TGTATTTGAT GAGAACCGAA GCTGGTACCT CACAGAGAAT
    ATACAACGCT TTCTCCCCAA TCCAGCTGGA GTGCAGCTTG AGGATCCAGA GTTCCAAGCC
    TCCAACATCA TGCACAGCAT CAATGGCTAT GTTTTTGATA GTTTGCAGTT GTCAGTTTGT
    TTGCATGAGG TGGCATACTG GTACATTCTA AGCATTGGAG CACAGACTGA CTTCCTTTCT
    GTCTTCTTCT CTGGATATAC CTTCAAACAC AAAATGGTCT ATGAAGATAC ACTCACCCTA
    TTCCCATTCT CAGGAGAAAC TGTCTTCATG TCGATGGAAA ACCCAGGCCT ATGGATTCTG
    GGGTGCCACA ACTCAGACTT TCGGAACAGA GGCATGACCG CCTTACTGAA GGTTTCTAGT
    TGTGACAAGA ACACTGGTGA TTATTACGAG GACAGTTATG AAGATATTTC AGCATACTTG
    CTGAGTAAAA ACAATGCCAT TGAACCAAGA AGCTTCTCCC AGAATTCAAG ACACCCTAGC
    ACTAGGCAAA AGCAATTTAA TGCCACCACA ATTCCAGAAA ATGACATAGA GAAGACTGAC
    CCTTGGTTTG CACACAGAAC ACCTATGCCT AAAGTACAAA ATGTCTCCTC TAGTGATTTG
    TTGATGCTCT TGCGACAGAG TCCTACTCCA CATGGGCTAT CCTTATCTGA TCTCCAAGAA
    GCCAAATATG AGACTTTTTC TGATGATCCA TCACCTGGAG CAATAGACAG TAATAACAGC
    CTATCTGAAA TGACACACTT CAGGCCACAG TTCCATCACA GTGGGGACAT GGTATTTACC
    CCTGAGTCAG GCCTCCAATT GAGATTAAAT GAGAAACTGG GGACAACTGC AGCAACAGAG
    TTGAAGAAAC TTGATTTCAA AGTTTCTAGT ACATCAAATA ATCTGATTTC AACAATTCCA
    TCAGACAATT TGGCAGCAGG TACTGATAAT ACAAGTTCCT TAGGACCCCC AAGTATGCCA
    GTTCATTATG ATAGTCAATT AGATACCACT CTATTTGGCA AAAAGTCATC TCCCCTTACT
    GAGTCTGGTA GACCTCTGAG CTTGAGTGAA GAAAATAATG ATTCAAAGTT GTTAGAATCA
    GGTTTAATGA ATAGCCAAGA AAGTTCATGG GGAAAAAATG TATCGTCAAC AGAGAGTGGT
    AGGTTATTTA AAGGGAAAAG AGCTCATGGA CCTGCTTTGT TGACTAAAGA TAATGCCTTA
    TTCAAAGTTA GCATCTCTTT GTTAAAGACA AACAAAACTT CCAATAATTC AGCAACTAAT
    AGAAAGACTC ACATTGATGG CCCATCATTA TTAATTCAGA GTAGTCCATC AGTCTGGCAA
    AATATATTAG AAAGTGACAC TGAGTTTAAA AAAGTGACAC CTTTGATTCA TGACAGAATG
    CTTATGGACA AAAATGCTAC AGCTTTGAGG CTAAATCATA TGTCAAATAA AACTACTTCA
    TCAAAAAACA TGGAAATGGT CCGACAGAAA AAAGAGGGCC CCATTCCACC AGATGCAGAA
    AATCCAGATA TGTCATTCTT TAAGATGCTA TTCTTGCCAG AATCAGCAAG TTGGATACAA
    AGGACTCATG GAAAGAACTC TCTGAACTCT GGGCAAGGCC CCAGTCCAAA GCAATTAGTA
    TCCTTAGGAC CAGAAAAATC TGTGGAAGGT CAGAATTTCT TGTCTGAGAA AAACAAAGTG
    GTAGTAGGAA AGGGTGAATT TACAAAGGAC GTAGGACTCA AAGAGATGGT TTTTCCAAGC
    AGCAGAAACC TATTTCTTAC TAACTTGGAT AATTTACATG AAAATAATAC ACACAATCAA
    GAAAAAAAAA TTCAGGAAGA AATAGAAAGG AAGGAAACAT TAATCCAAGA GAATGTAGTT
    TTGCCTCAGA TACATACAGT GACTGGCACT AAGAATTTCA TGAAGAACCT TTTCTTACTG
    AGCACTAGGC AAAATGTAGA AGGTTCATAT GAGGGGGCAT ATGCTCCAGT ACTTCAAGAT
    TTTAGGTCAT TAAATGATTC AACAAATAGA ACAAAGAAAC ACACGGCTCA TTTCTCAAAA
    AAAGGGGAAG AAGAAAACTT GGCAGGCTTG GGAAATCAAA CCAAGCAAAT TGTAGAGAAA
    TATGCATGCA CCACAAGGAT ATCTCCTAAT ACAAGCCAGC AGAATTTTGT CACGCAACGT
    AGTAAGAGAG CTTTGAAACA ATTCAGACTC CCACTAGAAG AAACAGAACT TGAAAAAAGG
    ATAATTGTGG ATGACACCTC AACCCAGTGG TCCAAAAACA TGAAACATTT GACCCCGAGC
    ACCCTCACAC AGATAGACTA CAATGAGAAG GAGAAAGGGG CCATTACTCA GTCTCCCTTA
    TCAGATTGCC TTACGAGGAG TCATAGCATC CCTCAAGCAA ATAGATCTCC ATTACCCATT
    GCAGAGGTAT CATCATTTCC ATCTATTAGA CCTATATATC TGACCAGGGT CCTATTCCAA
    GACAACTCTT CTCATCTTCC AGCAGCATCT TATAGAAAGA AAGATTCTGG GGTCCAAGAA
    AGCAGTCATT TCTTACAAGG AGCCAAAAAA AATAACCTTT CTTTAGCCAT TCTAACCTTG
    GAGATGACTG GTGATCAAAG AGAGGTTGGC TCCCTGGGGA CAAGTGCCAC AAATTCAGTC
    ACTTACAAGA AAGTTGAGAA CACTGTTCTC CCGAAACCAG GCTTGCCCAA AACATCTGGC
    AAAGTTGAAT TGCTTCCAAA AGTTCACATT TATCAGAAGG ACCTATTCCC TACGGAAACT
    AGCAATGGGT CTCCTGGCCA TCTGGATCTC GTGGAAGGGA GCCTTCTTCA GGAAACAGAG
    GGAGCGATTA AGTGGAATGA AGCAAACAGA CCTGGAAAAA TTCCCTTTCT GAGAGTAGCA
    ACAGAAAGCT CTGCAAAGAC TCCCTCCAAG CTATTGGATC CTCTTGCTTG GGATAACCAC
    TATGGTACTC AGATACCAAA AGAAGAGTGG AAATCCCAAG AGAAGTCACC AGAAAAAACA
    GCTTTTAAGA AAAAGGATAC CATTTTGTCC CTGAACGCTT GTGAAAGCAA TCATGCAATA
    GCAGCAATAA ATGAGGGACA AAATAAGCCC GAAATAGAAG TCACCTGGGC AAAGCAAGGT
    AGGACTGAAA GGCTGTGCTC TCAAAACCCA CCAGGCTTGA AACGCCATCA ACGGGAAATA
    ACTCGTACTA CTCTTCAGTC AGATCAAGAG GAAATTGACT ATGATGATAC CATCTCAGTT
    GAAATGAAGA AGGAAGATTT TGACATTTAT GATGAGGATG AAAATCAGAG CCCCCGCAGC
    TTTCAAAAGA AAACACGACA CTATTTTATT GCTGCAGTGG AGAGGCTCTG GGATTATGGG
    ATGAGTAGCT CCCCACATGT TCTAAGAAAC AGGGCTCAGA GTGGCAGTGT CCCTCAGTTC
    AAGAAAGTTG TTTTCCAGGA ATTTACTGAT GGCTCCTTTA CTCAGCCCTT ATACCGTGGA
    GAACTAAATG AACATTTGGG ACTCCTGGGG CCATATATAA GAGCAGAAGT TGAAGATAAT
    ATCATGGTAA CTTTCAAAAA TCAGGCCTCT CGTCCCTATT CCTTCTATTC TAGCCTTATT
    TCTTATGAGG AAGATCAGAG GCAAGGAGCA GAACCTAGGA AAAACTTTGT CAAGCCTAAT
    GAAACCAAAA CTTACTTTTG GAAAGTGCAA CATCATATGG CGCCCACTAA AGATGAGTTT
    GACTGCAAAG CCTGGGCTTA TTTCTCTGAT GTTGACCTGG AAAAAGATGT GCACTCAGGC
    CTGATTGGAC CCCTTCTGGT CTGCCACACT AACACACTGA ACCCTGCTCA TGGGAGACAA
    GTGACAGTAC AGGAATTTGC TCTGTTTTTC ACCATCTTTG ATGAGACCAA AAGCTGGTAC
    TTCACTGAAA ACATGGAAAG AAACTGCAGG GCTCCCTGCA ATATCCAGAT GGAAGATCCC
    ACTTTTAAAG AGAATTATCG CTTCCATGCA ATCAATGGCT ACATAATGGA TACACTACCT
    GGCTTAGTAA TGGCTCAGGA TCAAAGGATT CGATGGTATC TGCTCAGCAT GGGCAGCAAT
    GAAAACATCC ATTCTATTCA TTTCAGTGGA CATGTGTTCA CTGTACGAAA AAAAGAGGAG
    TATAAAATGG CACTGTACAA TCTCTATCCA GGTGTTTTTG AGACAGTGGA AATGTTACCA
    TCCAAAGCTG GAATTTGGCG GGTGGAATGC CTTATTGGCG AGCATCTACA TGCTGGGATG
    AGCACACTTT TTCTGGTGTA CAGCAATAAG TGTCAGACTC CCCTGGGAAT GGCTTCTGGA
    CACATTAGAG ATTTTCAGAT TACAGCTTCA GGACAATATG GACAGTGGGC CCCAAAGCTG
    GCCAGACTTC ATTATTCCGG ATCAATCAAT GCCTGGAGCA CCAAGGAGCC CTTTTCTTGG
    ATCAAGGTGG ATCTGTTGGC ACCAATGATT ATTCACGGCA TCAAGACCCA GGGTGCCCGT
    CAGAAGTTCT CCAGCCTCTA CATCTCTCAG TTTATCATCA TGTATAGTCT TGATGGGAAG
    AAGTGGCAGA CTTATCGAGG AAATTCCACT GGAACCTTAA TGGTCTTCTT TGGCAATGTG
    GATTCATCTG GGATAAAACA CAATATTTTT AACCCTCCAA TTATTGCTCG ATACATCCGT
    TTGCACCCAA CTCATTATAG CATTCGCAGC ACTCTTCGCA TGGAGTTGAT GGGCTGTGAT
    TTAAATAGTT GCAGCATGCC ATTGGGAATG GAGAGTAAAG CAATATCAGA TGCACAGATT
    ACTGCTTCAT CCTACTTTAC CAATATGTTT GCCACCTGGT CTCCTTCAAA AGCTCGACTT
    CACCTCCAAG GGAGGAGTAA TGCCTGGAGA CCTCAGGTGA ATAATCCAAA AGAGTGGCTG
    CAAGTGGACT TCCAGAAGAC AATGAAAGTC ACAGGAATAA CTACTCAGGG AGTAAAATCT
    CTGCTTACCA GCATGTATGT GAAGGAGTTC CTCATCTCCA GCAGTCAAGA TGGCCATCAC
    TGGACTCTCT TTTTTCAGAA TGGCAAAGTA AAGGTTTTTC AGGGAAATCA AGACTCCTTC
    ACACCTGTGG TGAACTCTCT AGACCCACCG TTACTGACTC GCTACCTTCG AATTCACCCC
    CAGAGTTGGG TGCACCAGAT TGCCCTGAGG ATGGAGGTTC TGGGCTGCGA GGCACAGGAC
    CTCTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016800114.1
    CDS137..6865
    Translation

    Target ORF information:

    RefSeq Version XM_016944625.2
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes coagulation factor VIII (F8), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016944625.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGCTTCCC ATCCTGTCAG TCTTCATGCT GTTGGTGTAT CCTACTGGAA AGCTTCTGAG 
    GGAGCTGAAT ATGATGATCA GACCAGTCAA AGGGAGAAAG AAGATGATAA AGTCTTTCCT
    GGTGGAAGCC ATACATATGT CTGGCAGGTC CTGAAAGAGA ATGGTCCAAT GGCCTCTGAC
    CCACTGTGCC TTACCTACTC ATATCTTTCT CATGTGGACC TGGTCAAAGA CTTGAATTCA
    GGCCTCATTG GAGCCCTACT AGTATGTAGA GAAGGGAGTC TGGCCAAGGA AAAGACACAG
    ACCTTGCACA AATTTATACT ACTTTTTGCT GTATTTGATG AAGGGAAAAG TTGGCACTCA
    GAAACAAAGA ACTCCTTGAT GCAGGATAGG GATGCTGCAT CTGCTCGGGC CTGGCCTAAA
    ATGCACACAG TCAATGGTTA TGTAAACAGG TCTCTGCCAG GTCTGATTGG ATGCCACAGG
    AAATCAGTCT ATTGGCATGT GATTGGAATG GGCACCACTC CTGAAGTGCA CTCAATATTC
    CTCGAAGGTC ACACATTTCT TGTGAGGAAC CATCGCCAGG CGTCCTTGGA AATATCGCCA
    ATAACTTTCC TTACTGCTCA AACACTCTTG ATGGACCTTG GACAGTTTCT ACTGTTTTGT
    CATATCTCTT CCCACCAACA TGATGGCATG GAAGCTTATG TCAAAGTAGA CAGCTGCCCA
    GAGGAACCCC AACTACGAAT GAAAAATAAT GAAGAAGCGG AAGACTATGA TGATGATCTT
    ACTGATTCTG AAATGGATGT GGTCAGGTTT GATGATGACA ACTCTCCTTC CTTTATCCAA
    ATTCGCTCAG TTGCCAAGAA GCATCCTAAA ACTTGGGTAC ATTACATTGC TGCTGAAGAG
    GAGGACTGGG ACTATGCTCC CTTAGTCCTC GCCCCCGATG ACAGAAGTTA TAAAAGTCAA
    TATTTGAACA ATGGCCCTCA GCGGATTGGT AGGAAGTACA AAAAAGTCCG ATTTATGGCA
    TACACAGATG AAACCTTTAA GACTCGTGAA GCTATTCAGC ATGAATCAGG AATCTTGGGA
    CCTTTACTTT ATGGGGAAGT TGGAGACACA CTGTTGATTA TATTTAAGAA TCAAGCAAGC
    AGACCATATA ACATCTACCC TCACGGAATC ACTGATGTCC GTCCTTTGTA TTCAAGGAGA
    TTACCAAAAG GTGTAAAACA TTTGAAGGAT TTTCCAATTC TGCCAGGAGA AATATTCAAA
    TATAAATGGA CAGTGACTGT AGAAGATGGG CCAACTAAAT CAGATCCTCG GTGCCTGACC
    CGCTATTACT CTAGTTTCGT TAATATGGAG AGAGATCTAG CTTCAGGACT CATTGGCCCT
    CTCCTCATCT GCTACAAAGA ATCTGTAGAT CAAAGAGGAA ACCAGATAAT GTCAGACAAG
    AGGAATGTCA TCCTGTTTTC TGTATTTGAT GAGAACCGAA GCTGGTACCT CACAGAGAAT
    ATACAACGCT TTCTCCCCAA TCCAGCTGGA GTGCAGCTTG AGGATCCAGA GTTCCAAGCC
    TCCAACATCA TGCACAGCAT CAATGGCTAT GTTTTTGATA GTTTGCAGTT GTCAGTTTGT
    TTGCATGAGG TGGCATACTG GTACATTCTA AGCATTGGAG CACAGACTGA CTTCCTTTCT
    GTCTTCTTCT CTGGATATAC CTTCAAACAC AAAATGGTCT ATGAAGATAC ACTCACCCTA
    TTCCCATTCT CAGGAGAAAC TGTCTTCATG TCGATGGAAA ACCCAGGCCT ATGGATTCTG
    GGGTGCCACA ACTCAGACTT TCGGAACAGA GGCATGACCG CCTTACTGAA GGTTTCTAGT
    TGTGACAAGA ACACTGGTGA TTATTACGAG GACAGTTATG AAGATATTTC AGCATACTTG
    CTGAGTAAAA ACAATGCCAT TGAACCAAGA AGCTTCTCCC AGAATTCAAG ACACCCTAGC
    ACTAGGCAAA AGCAATTTAA TGCCACCACA ATTCCAGAAA ATGACATAGA GAAGACTGAC
    CCTTGGTTTG CACACAGAAC ACCTATGCCT AAAGTACAAA ATGTCTCCTC TAGTGATTTG
    TTGATGCTCT TGCGACAGAG TCCTACTCCA CATGGGCTAT CCTTATCTGA TCTCCAAGAA
    GCCAAATATG AGACTTTTTC TGATGATCCA TCACCTGGAG CAATAGACAG TAATAACAGC
    CTATCTGAAA TGACACACTT CAGGCCACAG TTCCATCACA GTGGGGACAT GGTATTTACC
    CCTGAGTCAG GCCTCCAATT GAGATTAAAT GAGAAACTGG GGACAACTGC AGCAACAGAG
    TTGAAGAAAC TTGATTTCAA AGTTTCTAGT ACATCAAATA ATCTGATTTC AACAATTCCA
    TCAGACAATT TGGCAGCAGG TACTGATAAT ACAAGTTCCT TAGGACCCCC AAGTATGCCA
    GTTCATTATG ATAGTCAATT AGATACCACT CTATTTGGCA AAAAGTCATC TCCCCTTACT
    GAGTCTGGTA GACCTCTGAG CTTGAGTGAA GAAAATAATG ATTCAAAGTT GTTAGAATCA
    GGTTTAATGA ATAGCCAAGA AAGTTCATGG GGAAAAAATG TATCGTCAAC AGAGAGTGGT
    AGGTTATTTA AAGGGAAAAG AGCTCATGGA CCTGCTTTGT TGACTAAAGA TAATGCCTTA
    TTCAAAGTTA GCATCTCTTT GTTAAAGACA AACAAAACTT CCAATAATTC AGCAACTAAT
    AGAAAGACTC ACATTGATGG CCCATCATTA TTAATTCAGA GTAGTCCATC AGTCTGGCAA
    AATATATTAG AAAGTGACAC TGAGTTTAAA AAAGTGACAC CTTTGATTCA TGACAGAATG
    CTTATGGACA AAAATGCTAC AGCTTTGAGG CTAAATCATA TGTCAAATAA AACTACTTCA
    TCAAAAAACA TGGAAATGGT CCGACAGAAA AAAGAGGGCC CCATTCCACC AGATGCAGAA
    AATCCAGATA TGTCATTCTT TAAGATGCTA TTCTTGCCAG AATCAGCAAG TTGGATACAA
    AGGACTCATG GAAAGAACTC TCTGAACTCT GGGCAAGGCC CCAGTCCAAA GCAATTAGTA
    TCCTTAGGAC CAGAAAAATC TGTGGAAGGT CAGAATTTCT TGTCTGAGAA AAACAAAGTG
    GTAGTAGGAA AGGGTGAATT TACAAAGGAC GTAGGACTCA AAGAGATGGT TTTTCCAAGC
    AGCAGAAACC TATTTCTTAC TAACTTGGAT AATTTACATG AAAATAATAC ACACAATCAA
    GAAAAAAAAA TTCAGGAAGA AATAGAAAGG AAGGAAACAT TAATCCAAGA GAATGTAGTT
    TTGCCTCAGA TACATACAGT GACTGGCACT AAGAATTTCA TGAAGAACCT TTTCTTACTG
    AGCACTAGGC AAAATGTAGA AGGTTCATAT GAGGGGGCAT ATGCTCCAGT ACTTCAAGAT
    TTTAGGTCAT TAAATGATTC AACAAATAGA ACAAAGAAAC ACACGGCTCA TTTCTCAAAA
    AAAGGGGAAG AAGAAAACTT GGCAGGCTTG GGAAATCAAA CCAAGCAAAT TGTAGAGAAA
    TATGCATGCA CCACAAGGAT ATCTCCTAAT ACAAGCCAGC AGAATTTTGT CACGCAACGT
    AGTAAGAGAG CTTTGAAACA ATTCAGACTC CCACTAGAAG AAACAGAACT TGAAAAAAGG
    ATAATTGTGG ATGACACCTC AACCCAGTGG TCCAAAAACA TGAAACATTT GACCCCGAGC
    ACCCTCACAC AGATAGACTA CAATGAGAAG GAGAAAGGGG CCATTACTCA GTCTCCCTTA
    TCAGATTGCC TTACGAGGAG TCATAGCATC CCTCAAGCAA ATAGATCTCC ATTACCCATT
    GCAGAGGTAT CATCATTTCC ATCTATTAGA CCTATATATC TGACCAGGGT CCTATTCCAA
    GACAACTCTT CTCATCTTCC AGCAGCATCT TATAGAAAGA AAGATTCTGG GGTCCAAGAA
    AGCAGTCATT TCTTACAAGG AGCCAAAAAA AATAACCTTT CTTTAGCCAT TCTAACCTTG
    GAGATGACTG GTGATCAAAG AGAGGTTGGC TCCCTGGGGA CAAGTGCCAC AAATTCAGTC
    ACTTACAAGA AAGTTGAGAA CACTGTTCTC CCGAAACCAG GCTTGCCCAA AACATCTGGC
    AAAGTTGAAT TGCTTCCAAA AGTTCACATT TATCAGAAGG ACCTATTCCC TACGGAAACT
    AGCAATGGGT CTCCTGGCCA TCTGGATCTC GTGGAAGGGA GCCTTCTTCA GGAAACAGAG
    GGAGCGATTA AGTGGAATGA AGCAAACAGA CCTGGAAAAA TTCCCTTTCT GAGAGTAGCA
    ACAGAAAGCT CTGCAAAGAC TCCCTCCAAG CTATTGGATC CTCTTGCTTG GGATAACCAC
    TATGGTACTC AGATACCAAA AGAAGAGTGG AAATCCCAAG AGAAGTCACC AGAAAAAACA
    GCTTTTAAGA AAAAGGATAC CATTTTGTCC CTGAACGCTT GTGAAAGCAA TCATGCAATA
    GCAGCAATAA ATGAGGGACA AAATAAGCCC GAAATAGAAG TCACCTGGGC AAAGCAAGGT
    AGGACTGAAA GGCTGTGCTC TCAAAACCCA CCAGGCTTGA AACGCCATCA ACGGGAAATA
    ACTCGTACTA CTCTTCAGTC AGATCAAGAG GAAATTGACT ATGATGATAC CATCTCAGTT
    GAAATGAAGA AGGAAGATTT TGACATTTAT GATGAGGATG AAAATCAGAG CCCCCGCAGC
    TTTCAAAAGA AAACACGACA CTATTTTATT GCTGCAGTGG AGAGGCTCTG GGATTATGGG
    ATGAGTAGCT CCCCACATGT TCTAAGAAAC AGGGCTCAGA GTGGCAGTGT CCCTCAGTTC
    AAGAAAGTTG TTTTCCAGGA ATTTACTGAT GGCTCCTTTA CTCAGCCCTT ATACCGTGGA
    GAACTAAATG AACATTTGGG ACTCCTGGGG CCATATATAA GAGCAGAAGT TGAAGATAAT
    ATCATGGTAA CTTTCAAAAA TCAGGCCTCT CGTCCCTATT CCTTCTATTC TAGCCTTATT
    TCTTATGAGG AAGATCAGAG GCAAGGAGCA GAACCTAGGA AAAACTTTGT CAAGCCTAAT
    GAAACCAAAA CTTACTTTTG GAAAGTGCAA CATCATATGG CGCCCACTAA AGATGAGTTT
    GACTGCAAAG CCTGGGCTTA TTTCTCTGAT GTTGACCTGG AAAAAGATGT GCACTCAGGC
    CTGATTGGAC CCCTTCTGGT CTGCCACACT AACACACTGA ACCCTGCTCA TGGGAGACAA
    GTGACAGTAC AGGAATTTGC TCTGTTTTTC ACCATCTTTG ATGAGACCAA AAGCTGGTAC
    TTCACTGAAA ACATGGAAAG AAACTGCAGG GCTCCCTGCA ATATCCAGAT GGAAGATCCC
    ACTTTTAAAG AGAATTATCG CTTCCATGCA ATCAATGGCT ACATAATGGA TACACTACCT
    GGCTTAGTAA TGGCTCAGGA TCAAAGGATT CGATGGTATC TGCTCAGCAT GGGCAGCAAT
    GAAAACATCC ATTCTATTCA TTTCAGTGGA CATGTGTTCA CTGTACGAAA AAAAGAGGAG
    TATAAAATGG CACTGTACAA TCTCTATCCA GGTGTTTTTG AGACAGTGGA AATGTTACCA
    TCCAAAGCTG GAATTTGGCG GGTGGAATGC CTTATTGGCG AGCATCTACA TGCTGGGATG
    AGCACACTTT TTCTGGTGTA CAGCAATAAG TGTCAGACTC CCCTGGGAAT GGCTTCTGGA
    CACATTAGAG ATTTTCAGAT TACAGCTTCA GGACAATATG GACAGTGGGC CCCAAAGCTG
    GCCAGACTTC ATTATTCCGG ATCAATCAAT GCCTGGAGCA CCAAGGAGCC CTTTTCTTGG
    ATCAAGGTGG ATCTGTTGGC ACCAATGATT ATTCACGGCA TCAAGACCCA GGGTGCCCGT
    CAGAAGTTCT CCAGCCTCTA CATCTCTCAG TTTATCATCA TGTATAGTCT TGATGGGAAG
    AAGTGGCAGA CTTATCGAGG AAATTCCACT GGAACCTTAA TGGTCTTCTT TGGCAATGTG
    GATTCATCTG GGATAAAACA CAATATTTTT AACCCTCCAA TTATTGCTCG ATACATCCGT
    TTGCACCCAA CTCATTATAG CATTCGCAGC ACTCTTCGCA TGGAGTTGAT GGGCTGTGAT
    TTAAATAGTT GCAGCATGCC ATTGGGAATG GAGAGTAAAG CAATATCAGA TGCACAGATT
    ACTGCTTCAT CCTACTTTAC CAATATGTTT GCCACCTGGT CTCCTTCAAA AGCTCGACTT
    CACCTCCAAG GGAGGAGTAA TGCCTGGAGA CCTCAGGTGA ATAATCCAAA AGAGTGGCTG
    CAAGTGGACT TCCAGAAGAC AATGAAAGTC ACAGGAATAA CTACTCAGGG AGTAAAATCT
    CTGCTTACCA GCATGTATGT GAAGGAGTTC CTCATCTCCA GCAGTCAAGA TGGCCATCAC
    TGGACTCTCT TTTTTCAGAA TGGCAAAGTA AAGGTTTTTC AGGGAAATCA AGACTCCTTC
    ACACCTGTGG TGAACTCTCT AGACCCACCG TTACTGACTC GCTACCTTCG AATTCACCCC
    CAGAGTTGGG TGCACCAGAT TGCCCTGAGG ATGGAGGTTC TGGGCTGCGA GGCACAGGAC
    CTCTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc25432
    Clone ID Related Accession (Same CDS sequence) XM_003317789.3 , XM_003317789.4
    Accession Version XM_003317789.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7056bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product coagulation factor VIII isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006491.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_003317789.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCAAATAG AGCTCTCCAC CTGCTTCTTT CTGTGCCTTT TGCGATTCTG CTTTAGTGCC 
    ACCAGAAGAT ACTACCTGGG TGCAGTGGAA CTGTCATGGG ACTATATGCA AAGTGATCTC
    GGTGAGCTGC CTGTGGACGC AAGATTTCCT CCTAGAGTGC CAAAATCTTT TCCATTCAAC
    ACCTCAGTCG TGTACAAAAA GACTGTGTTT GTAGAATTCA CGGATCACCT TTTCAACATC
    GCTAAGCCAA GGCCACCCTG GATGGGTCTG CTAGGTCCTA CCATCCAGGC TGAGGTTTAT
    GATACAGTGG TCATTACACT TAAGAACATG GCTTCCCATC CTGTCAGTCT TCATGCTGTT
    GGTGTATCCT ACTGGAAAGC TTCTGAGGGA GCTGAATATG ATGATCAGAC CAGTCAAAGG
    GAGAAAGAAG ATGATAAAGT CTTTCCTGGT GGAAGCCATA CATATGTCTG GCAGGTCCTG
    AAAGAGAATG GTCCAATGGC CTCTGACCCA CTGTGCCTTA CCTACTCATA TCTTTCTCAT
    GTGGACCTGG TCAAAGACTT GAATTCAGGC CTCATTGGAG CCCTACTAGT ATGTAGAGAA
    GGGAGTCTGG CCAAGGAAAA GACACAGACC TTGCACAAAT TTATACTACT TTTTGCTGTA
    TTTGATGAAG GGAAAAGTTG GCACTCAGAA ACAAAGAACT CCTTGATGCA GGATAGGGAT
    GCTGCATCTG CTCGGGCCTG GCCTAAAATG CACACAGTCA ATGGTTATGT AAACAGGTCT
    CTGCCAGGTC TGATTGGATG CCACAGGAAA TCAGTCTATT GGCATGTGAT TGGAATGGGC
    ACCACTCCTG AAGTGCACTC AATATTCCTC GAAGGTCACA CATTTCTTGT GAGGAACCAT
    CGCCAGGCGT CCTTGGAAAT ATCGCCAATA ACTTTCCTTA CTGCTCAAAC ACTCTTGATG
    GACCTTGGAC AGTTTCTACT GTTTTGTCAT ATCTCTTCCC ACCAACATGA TGGCATGGAA
    GCTTATGTCA AAGTAGACAG CTGCCCAGAG GAACCCCAAC TACGAATGAA AAATAATGAA
    GAAGCGGAAG ACTATGATGA TGATCTTACT GATTCTGAAA TGGATGTGGT CAGGTTTGAT
    GATGACAACT CTCCTTCCTT TATCCAAATT CGCTCAGTTG CCAAGAAGCA TCCTAAAACT
    TGGGTACATT ACATTGCTGC TGAAGAGGAG GACTGGGACT ATGCTCCCTT AGTCCTCGCC
    CCCGATGACA GAAGTTATAA AAGTCAATAT TTGAACAATG GCCCTCAGCG GATTGGTAGG
    AAGTACAAAA AAGTCCGATT TATGGCATAC ACAGATGAAA CCTTTAAGAC TCGTGAAGCT
    ATTCAGCATG AATCAGGAAT CTTGGGACCT TTACTTTATG GGGAAGTTGG AGACACACTG
    TTGATTATAT TTAAGAATCA AGCAAGCAGA CCATATAACA TCTACCCTCA CGGAATCACT
    GATGTCCGTC CTTTGTATTC AAGGAGATTA CCAAAAGGTG TAAAACATTT GAAGGATTTT
    CCAATTCTGC CAGGAGAAAT ATTCAAATAT AAATGGACAG TGACTGTAGA AGATGGGCCA
    ACTAAATCAG ATCCTCGGTG CCTGACCCGC TATTACTCTA GTTTCGTTAA TATGGAGAGA
    GATCTAGCTT CAGGACTCAT TGGCCCTCTC CTCATCTGCT ACAAAGAATC TGTAGATCAA
    AGAGGAAACC AGATAATGTC AGACAAGAGG AATGTCATCC TGTTTTCTGT ATTTGATGAG
    AACCGAAGCT GGTACCTCAC AGAGAATATA CAACGCTTTC TCCCCAATCC AGCTGGAGTG
    CAGCTTGAGG ATCCAGAGTT CCAAGCCTCC AACATCATGC ACAGCATCAA TGGCTATGTT
    TTTGATAGTT TGCAGTTGTC AGTTTGTTTG CATGAGGTGG CATACTGGTA CATTCTAAGC
    ATTGGAGCAC AGACTGACTT CCTTTCTGTC TTCTTCTCTG GATATACCTT CAAACACAAA
    ATGGTCTATG AAGATACACT CACCCTATTC CCATTCTCAG GAGAAACTGT CTTCATGTCG
    ATGGAAAACC CAGGCCTATG GATTCTGGGG TGCCACAACT CAGACTTTCG GAACAGAGGC
    ATGACCGCCT TACTGAAGGT TTCTAGTTGT GACAAGAACA CTGGTGATTA TTACGAGGAC
    AGTTATGAAG ATATTTCAGC ATACTTGCTG AGTAAAAACA ATGCCATTGA ACCAAGAAGC
    TTCTCCCAGA ATTCAAGACA CCCTAGCACT AGGCAAAAGC AATTTAATGC CACCACAATT
    CCAGAAAATG ACATAGAGAA GACTGACCCT TGGTTTGCAC ACAGAACACC TATGCCTAAA
    GTACAAAATG TCTCCTCTAG TGATTTGTTG ATGCTCTTGC GACAGAGTCC TACTCCACAT
    GGGCTATCCT TATCTGATCT CCAAGAAGCC AAATATGAGA CTTTTTCTGA TGATCCATCA
    CCTGGAGCAA TAGACAGTAA TAACAGCCTA TCTGAAATGA CACACTTCAG GCCACAGTTC
    CATCACAGTG GGGACATGGT ATTTACCCCT GAGTCAGGCC TCCAATTGAG ATTAAATGAG
    AAACTGGGGA CAACTGCAGC AACAGAGTTG AAGAAACTTG ATTTCAAAGT TTCTAGTACA
    TCAAATAATC TGATTTCAAC AATTCCATCA GACAATTTGG CAGCAGGTAC TGATAATACA
    AGTTCCTTAG GACCCCCAAG TATGCCAGTT CATTATGATA GTCAATTAGA TACCACTCTA
    TTTGGCAAAA AGTCATCTCC CCTTACTGAG TCTGGTAGAC CTCTGAGCTT GAGTGAAGAA
    AATAATGATT CAAAGTTGTT AGAATCAGGT TTAATGAATA GCCAAGAAAG TTCATGGGGA
    AAAAATGTAT CGTCAACAGA GAGTGGTAGG TTATTTAAAG GGAAAAGAGC TCATGGACCT
    GCTTTGTTGA CTAAAGATAA TGCCTTATTC AAAGTTAGCA TCTCTTTGTT AAAGACAAAC
    AAAACTTCCA ATAATTCAGC AACTAATAGA AAGACTCACA TTGATGGCCC ATCATTATTA
    ATTCAGAGTA GTCCATCAGT CTGGCAAAAT ATATTAGAAA GTGACACTGA GTTTAAAAAA
    GTGACACCTT TGATTCATGA CAGAATGCTT ATGGACAAAA ATGCTACAGC TTTGAGGCTA
    AATCATATGT CAAATAAAAC TACTTCATCA AAAAACATGG AAATGGTCCG ACAGAAAAAA
    GAGGGCCCCA TTCCACCAGA TGCAGAAAAT CCAGATATGT CATTCTTTAA GATGCTATTC
    TTGCCAGAAT CAGCAAGTTG GATACAAAGG ACTCATGGAA AGAACTCTCT GAACTCTGGG
    CAAGGCCCCA GTCCAAAGCA ATTAGTATCC TTAGGACCAG AAAAATCTGT GGAAGGTCAG
    AATTTCTTGT CTGAGAAAAA CAAAGTGGTA GTAGGAAAGG GTGAATTTAC AAAGGACGTA
    GGACTCAAAG AGATGGTTTT TCCAAGCAGC AGAAACCTAT TTCTTACTAA CTTGGATAAT
    TTACATGAAA ATAATACACA CAATCAAGAA AAAAAAATTC AGGAAGAAAT AGAAAGGAAG
    GAAACATTAA TCCAAGAGAA TGTAGTTTTG CCTCAGATAC ATACAGTGAC TGGCACTAAG
    AATTTCATGA AGAACCTTTT CTTACTGAGC ACTAGGCAAA ATGTAGAAGG TTCATATGAG
    GGGGCATATG CTCCAGTACT TCAAGATTTT AGGTCATTAA ATGATTCAAC AAATAGAACA
    AAGAAACACA CGGCTCATTT CTCAAAAAAA GGGGAAGAAG AAAACTTGGC AGGCTTGGGA
    AATCAAACCA AGCAAATTGT AGAGAAATAT GCATGCACCA CAAGGATATC TCCTAATACA
    AGCCAGCAGA ATTTTGTCAC GCAACGTAGT AAGAGAGCTT TGAAACAATT CAGACTCCCA
    CTAGAAGAAA CAGAACTTGA AAAAAGGATA ATTGTGGATG ACACCTCAAC CCAGTGGTCC
    AAAAACATGA AACATTTGAC CCCGAGCACC CTCACACAGA TAGACTACAA TGAGAAGGAG
    AAAGGGGCCA TTACTCAGTC TCCCTTATCA GATTGCCTTA CGAGGAGTCA TAGCATCCCT
    CAAGCAAATA GATCTCCATT ACCCATTGCA GAGGTATCAT CATTTCCATC TATTAGACCT
    ATATATCTGA CCAGGGTCCT ATTCCAAGAC AACTCTTCTC ATCTTCCAGC AGCATCTTAT
    AGAAAGAAAG ATTCTGGGGT CCAAGAAAGC AGTCATTTCT TACAAGGAGC CAAAAAAAAT
    AACCTTTCTT TAGCCATTCT AACCTTGGAG ATGACTGGTG ATCAAAGAGA GGTTGGCTCC
    CTGGGGACAA GTGCCACAAA TTCAGTCACT TACAAGAAAG TTGAGAACAC TGTTCTCCCG
    AAACCAGGCT TGCCCAAAAC ATCTGGCAAA GTTGAATTGC TTCCAAAAGT TCACATTTAT
    CAGAAGGACC TATTCCCTAC GGAAACTAGC AATGGGTCTC CTGGCCATCT GGATCTCGTG
    GAAGGGAGCC TTCTTCAGGA AACAGAGGGA GCGATTAAGT GGAATGAAGC AAACAGACCT
    GGAAAAATTC CCTTTCTGAG AGTAGCAACA GAAAGCTCTG CAAAGACTCC CTCCAAGCTA
    TTGGATCCTC TTGCTTGGGA TAACCACTAT GGTACTCAGA TACCAAAAGA AGAGTGGAAA
    TCCCAAGAGA AGTCACCAGA AAAAACAGCT TTTAAGAAAA AGGATACCAT TTTGTCCCTG
    AACGCTTGTG AAAGCAATCA TGCAATAGCA GCAATAAATG AGGGACAAAA TAAGCCCGAA
    ATAGAAGTCA CCTGGGCAAA GCAAGGTAGG ACTGAAAGGC TGTGCTCTCA AAACCCACCA
    GGCTTGAAAC GCCATCAACG GGAAATAACT CGTACTACTC TTCAGTCAGA TCAAGAGGAA
    ATTGACTATG ATGATACCAT CTCAGTTGAA ATGAAGAAGG AAGATTTTGA CATTTATGAT
    GAGGATGAAA ATCAGAGCCC CCGCAGCTTT CAAAAGAAAA CACGACACTA TTTTATTGCT
    GCAGTGGAGA GGCTCTGGGA TTATGGGATG AGTAGCTCCC CACATGTTCT AAGAAACAGG
    GCTCAGAGTG GCAGTGTCCC TCAGTTCAAG AAAGTTGTTT TCCAGGAATT TACTGATGGC
    TCCTTTACTC AGCCCTTATA CCGTGGAGAA CTAAATGAAC ATTTGGGACT CCTGGGGCCA
    TATATAAGAG CAGAAGTTGA AGATAATATC ATGGTAACTT TCAAAAATCA GGCCTCTCGT
    CCCTATTCCT TCTATTCTAG CCTTATTTCT TATGAGGAAG ATCAGAGGCA AGGAGCAGAA
    CCTAGGAAAA ACTTTGTCAA GCCTAATGAA ACCAAAACTT ACTTTTGGAA AGTGCAACAT
    CATATGGCGC CCACTAAAGA TGAGTTTGAC TGCAAAGCCT GGGCTTATTT CTCTGATGTT
    GACCTGGAAA AAGATGTGCA CTCAGGCCTG ATTGGACCCC TTCTGGTCTG CCACACTAAC
    ACACTGAACC CTGCTCATGG GAGACAAGTG ACAGTACAGG AATTTGCTCT GTTTTTCACC
    ATCTTTGATG AGACCAAAAG CTGGTACTTC ACTGAAAACA TGGAAAGAAA CTGCAGGGCT
    CCCTGCAATA TCCAGATGGA AGATCCCACT TTTAAAGAGA ATTATCGCTT CCATGCAATC
    AATGGCTACA TAATGGATAC ACTACCTGGC TTAGTAATGG CTCAGGATCA AAGGATTCGA
    TGGTATCTGC TCAGCATGGG CAGCAATGAA AACATCCATT CTATTCATTT CAGTGGACAT
    GTGTTCACTG TACGAAAAAA AGAGGAGTAT AAAATGGCAC TGTACAATCT CTATCCAGGT
    GTTTTTGAGA CAGTGGAAAT GTTACCATCC AAAGCTGGAA TTTGGCGGGT GGAATGCCTT
    ATTGGCGAGC ATCTACATGC TGGGATGAGC ACACTTTTTC TGGTGTACAG CAATAAGTGT
    CAGACTCCCC TGGGAATGGC TTCTGGACAC ATTAGAGATT TTCAGATTAC AGCTTCAGGA
    CAATATGGAC AGTGGGCCCC AAAGCTGGCC AGACTTCATT ATTCCGGATC AATCAATGCC
    TGGAGCACCA AGGAGCCCTT TTCTTGGATC AAGGTGGATC TGTTGGCACC AATGATTATT
    CACGGCATCA AGACCCAGGG TGCCCGTCAG AAGTTCTCCA GCCTCTACAT CTCTCAGTTT
    ATCATCATGT ATAGTCTTGA TGGGAAGAAG TGGCAGACTT ATCGAGGAAA TTCCACTGGA
    ACCTTAATGG TCTTCTTTGG CAATGTGGAT TCATCTGGGA TAAAACACAA TATTTTTAAC
    CCTCCAATTA TTGCTCGATA CATCCGTTTG CACCCAACTC ATTATAGCAT TCGCAGCACT
    CTTCGCATGG AGTTGATGGG CTGTGATTTA AATAGTTGCA GCATGCCATT GGGAATGGAG
    AGTAAAGCAA TATCAGATGC ACAGATTACT GCTTCATCCT ACTTTACCAA TATGTTTGCC
    ACCTGGTCTC CTTCAAAAGC TCGACTTCAC CTCCAAGGGA GGAGTAATGC CTGGAGACCT
    CAGGTGAATA ATCCAAAAGA GTGGCTGCAA GTGGACTTCC AGAAGACAAT GAAAGTCACA
    GGAATAACTA CTCAGGGAGT AAAATCTCTG CTTACCAGCA TGTATGTGAA GGAGTTCCTC
    ATCTCCAGCA GTCAAGATGG CCATCACTGG ACTCTCTTTT TTCAGAATGG CAAAGTAAAG
    GTTTTTCAGG GAAATCAAGA CTCCTTCACA CCTGTGGTGA ACTCTCTAGA CCCACCGTTA
    CTGACTCGCT ACCTTCGAAT TCACCCCCAG AGTTGGGTGC ACCAGATTGC CCTGAGGATG
    GAGGTTCTGG GCTGCGAGGC ACAGGACCTC TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003317837.1
    CDS398..7453
    Translation

    Target ORF information:

    RefSeq Version XM_003317789.3
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes coagulation factor VIII (F8), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003317789.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCAAATAG AGCTCTCCAC CTGCTTCTTT CTGTGCCTTT TGCGATTCTG CTTTAGTGCC 
    ACCAGAAGAT ACTACCTGGG TGCAGTGGAA CTGTCATGGG ACTATATGCA AAGTGATCTC
    GGTGAGCTGC CTGTGGACGC AAGATTTCCT CCTAGAGTGC CAAAATCTTT TCCATTCAAC
    ACCTCAGTCG TGTACAAAAA GACTGTGTTT GTAGAATTCA CGGATCACCT TTTCAACATC
    GCTAAGCCAA GGCCACCCTG GATGGGTCTG CTAGGTCCTA CCATCCAGGC TGAGGTTTAT
    GATACAGTGG TCATTACACT TAAGAACATG GCTTCCCATC CTGTCAGTCT TCATGCTGTT
    GGTGTATCCT ACTGGAAAGC TTCTGAGGGA GCTGAATATG ATGATCAGAC CAGTCAAAGG
    GAGAAAGAAG ATGATAAAGT CTTTCCTGGT GGAAGCCATA CATATGTCTG GCAGGTCCTG
    AAAGAGAATG GTCCAATGGC CTCTGACCCA CTGTGCCTTA CCTACTCATA TCTTTCTCAT
    GTGGACCTGG TCAAAGACTT GAATTCAGGC CTCATTGGAG CCCTACTAGT ATGTAGAGAA
    GGGAGTCTGG CCAAGGAAAA GACACAGACC TTGCACAAAT TTATACTACT TTTTGCTGTA
    TTTGATGAAG GGAAAAGTTG GCACTCAGAA ACAAAGAACT CCTTGATGCA GGATAGGGAT
    GCTGCATCTG CTCGGGCCTG GCCTAAAATG CACACAGTCA ATGGTTATGT AAACAGGTCT
    CTGCCAGGTC TGATTGGATG CCACAGGAAA TCAGTCTATT GGCATGTGAT TGGAATGGGC
    ACCACTCCTG AAGTGCACTC AATATTCCTC GAAGGTCACA CATTTCTTGT GAGGAACCAT
    CGCCAGGCGT CCTTGGAAAT ATCGCCAATA ACTTTCCTTA CTGCTCAAAC ACTCTTGATG
    GACCTTGGAC AGTTTCTACT GTTTTGTCAT ATCTCTTCCC ACCAACATGA TGGCATGGAA
    GCTTATGTCA AAGTAGACAG CTGCCCAGAG GAACCCCAAC TACGAATGAA AAATAATGAA
    GAAGCGGAAG ACTATGATGA TGATCTTACT GATTCTGAAA TGGATGTGGT CAGGTTTGAT
    GATGACAACT CTCCTTCCTT TATCCAAATT CGCTCAGTTG CCAAGAAGCA TCCTAAAACT
    TGGGTACATT ACATTGCTGC TGAAGAGGAG GACTGGGACT ATGCTCCCTT AGTCCTCGCC
    CCCGATGACA GAAGTTATAA AAGTCAATAT TTGAACAATG GCCCTCAGCG GATTGGTAGG
    AAGTACAAAA AAGTCCGATT TATGGCATAC ACAGATGAAA CCTTTAAGAC TCGTGAAGCT
    ATTCAGCATG AATCAGGAAT CTTGGGACCT TTACTTTATG GGGAAGTTGG AGACACACTG
    TTGATTATAT TTAAGAATCA AGCAAGCAGA CCATATAACA TCTACCCTCA CGGAATCACT
    GATGTCCGTC CTTTGTATTC AAGGAGATTA CCAAAAGGTG TAAAACATTT GAAGGATTTT
    CCAATTCTGC CAGGAGAAAT ATTCAAATAT AAATGGACAG TGACTGTAGA AGATGGGCCA
    ACTAAATCAG ATCCTCGGTG CCTGACCCGC TATTACTCTA GTTTCGTTAA TATGGAGAGA
    GATCTAGCTT CAGGACTCAT TGGCCCTCTC CTCATCTGCT ACAAAGAATC TGTAGATCAA
    AGAGGAAACC AGATAATGTC AGACAAGAGG AATGTCATCC TGTTTTCTGT ATTTGATGAG
    AACCGAAGCT GGTACCTCAC AGAGAATATA CAACGCTTTC TCCCCAATCC AGCTGGAGTG
    CAGCTTGAGG ATCCAGAGTT CCAAGCCTCC AACATCATGC ACAGCATCAA TGGCTATGTT
    TTTGATAGTT TGCAGTTGTC AGTTTGTTTG CATGAGGTGG CATACTGGTA CATTCTAAGC
    ATTGGAGCAC AGACTGACTT CCTTTCTGTC TTCTTCTCTG GATATACCTT CAAACACAAA
    ATGGTCTATG AAGATACACT CACCCTATTC CCATTCTCAG GAGAAACTGT CTTCATGTCG
    ATGGAAAACC CAGGCCTATG GATTCTGGGG TGCCACAACT CAGACTTTCG GAACAGAGGC
    ATGACCGCCT TACTGAAGGT TTCTAGTTGT GACAAGAACA CTGGTGATTA TTACGAGGAC
    AGTTATGAAG ATATTTCAGC ATACTTGCTG AGTAAAAACA ATGCCATTGA ACCAAGAAGC
    TTCTCCCAGA ATTCAAGACA CCCTAGCACT AGGCAAAAGC AATTTAATGC CACCACAATT
    CCAGAAAATG ACATAGAGAA GACTGACCCT TGGTTTGCAC ACAGAACACC TATGCCTAAA
    GTACAAAATG TCTCCTCTAG TGATTTGTTG ATGCTCTTGC GACAGAGTCC TACTCCACAT
    GGGCTATCCT TATCTGATCT CCAAGAAGCC AAATATGAGA CTTTTTCTGA TGATCCATCA
    CCTGGAGCAA TAGACAGTAA TAACAGCCTA TCTGAAATGA CACACTTCAG GCCACAGTTC
    CATCACAGTG GGGACATGGT ATTTACCCCT GAGTCAGGCC TCCAATTGAG ATTAAATGAG
    AAACTGGGGA CAACTGCAGC AACAGAGTTG AAGAAACTTG ATTTCAAAGT TTCTAGTACA
    TCAAATAATC TGATTTCAAC AATTCCATCA GACAATTTGG CAGCAGGTAC TGATAATACA
    AGTTCCTTAG GACCCCCAAG TATGCCAGTT CATTATGATA GTCAATTAGA TACCACTCTA
    TTTGGCAAAA AGTCATCTCC CCTTACTGAG TCTGGTAGAC CTCTGAGCTT GAGTGAAGAA
    AATAATGATT CAAAGTTGTT AGAATCAGGT TTAATGAATA GCCAAGAAAG TTCATGGGGA
    AAAAATGTAT CGTCAACAGA GAGTGGTAGG TTATTTAAAG GGAAAAGAGC TCATGGACCT
    GCTTTGTTGA CTAAAGATAA TGCCTTATTC AAAGTTAGCA TCTCTTTGTT AAAGACAAAC
    AAAACTTCCA ATAATTCAGC AACTAATAGA AAGACTCACA TTGATGGCCC ATCATTATTA
    ATTCAGAGTA GTCCATCAGT CTGGCAAAAT ATATTAGAAA GTGACACTGA GTTTAAAAAA
    GTGACACCTT TGATTCATGA CAGAATGCTT ATGGACAAAA ATGCTACAGC TTTGAGGCTA
    AATCATATGT CAAATAAAAC TACTTCATCA AAAAACATGG AAATGGTCCG ACAGAAAAAA
    GAGGGCCCCA TTCCACCAGA TGCAGAAAAT CCAGATATGT CATTCTTTAA GATGCTATTC
    TTGCCAGAAT CAGCAAGTTG GATACAAAGG ACTCATGGAA AGAACTCTCT GAACTCTGGG
    CAAGGCCCCA GTCCAAAGCA ATTAGTATCC TTAGGACCAG AAAAATCTGT GGAAGGTCAG
    AATTTCTTGT CTGAGAAAAA CAAAGTGGTA GTAGGAAAGG GTGAATTTAC AAAGGACGTA
    GGACTCAAAG AGATGGTTTT TCCAAGCAGC AGAAACCTAT TTCTTACTAA CTTGGATAAT
    TTACATGAAA ATAATACACA CAATCAAGAA AAAAAAATTC AGGAAGAAAT AGAAAGGAAG
    GAAACATTAA TCCAAGAGAA TGTAGTTTTG CCTCAGATAC ATACAGTGAC TGGCACTAAG
    AATTTCATGA AGAACCTTTT CTTACTGAGC ACTAGGCAAA ATGTAGAAGG TTCATATGAG
    GGGGCATATG CTCCAGTACT TCAAGATTTT AGGTCATTAA ATGATTCAAC AAATAGAACA
    AAGAAACACA CGGCTCATTT CTCAAAAAAA GGGGAAGAAG AAAACTTGGC AGGCTTGGGA
    AATCAAACCA AGCAAATTGT AGAGAAATAT GCATGCACCA CAAGGATATC TCCTAATACA
    AGCCAGCAGA ATTTTGTCAC GCAACGTAGT AAGAGAGCTT TGAAACAATT CAGACTCCCA
    CTAGAAGAAA CAGAACTTGA AAAAAGGATA ATTGTGGATG ACACCTCAAC CCAGTGGTCC
    AAAAACATGA AACATTTGAC CCCGAGCACC CTCACACAGA TAGACTACAA TGAGAAGGAG
    AAAGGGGCCA TTACTCAGTC TCCCTTATCA GATTGCCTTA CGAGGAGTCA TAGCATCCCT
    CAAGCAAATA GATCTCCATT ACCCATTGCA GAGGTATCAT CATTTCCATC TATTAGACCT
    ATATATCTGA CCAGGGTCCT ATTCCAAGAC AACTCTTCTC ATCTTCCAGC AGCATCTTAT
    AGAAAGAAAG ATTCTGGGGT CCAAGAAAGC AGTCATTTCT TACAAGGAGC CAAAAAAAAT
    AACCTTTCTT TAGCCATTCT AACCTTGGAG ATGACTGGTG ATCAAAGAGA GGTTGGCTCC
    CTGGGGACAA GTGCCACAAA TTCAGTCACT TACAAGAAAG TTGAGAACAC TGTTCTCCCG
    AAACCAGGCT TGCCCAAAAC ATCTGGCAAA GTTGAATTGC TTCCAAAAGT TCACATTTAT
    CAGAAGGACC TATTCCCTAC GGAAACTAGC AATGGGTCTC CTGGCCATCT GGATCTCGTG
    GAAGGGAGCC TTCTTCAGGA AACAGAGGGA GCGATTAAGT GGAATGAAGC AAACAGACCT
    GGAAAAATTC CCTTTCTGAG AGTAGCAACA GAAAGCTCTG CAAAGACTCC CTCCAAGCTA
    TTGGATCCTC TTGCTTGGGA TAACCACTAT GGTACTCAGA TACCAAAAGA AGAGTGGAAA
    TCCCAAGAGA AGTCACCAGA AAAAACAGCT TTTAAGAAAA AGGATACCAT TTTGTCCCTG
    AACGCTTGTG AAAGCAATCA TGCAATAGCA GCAATAAATG AGGGACAAAA TAAGCCCGAA
    ATAGAAGTCA CCTGGGCAAA GCAAGGTAGG ACTGAAAGGC TGTGCTCTCA AAACCCACCA
    GGCTTGAAAC GCCATCAACG GGAAATAACT CGTACTACTC TTCAGTCAGA TCAAGAGGAA
    ATTGACTATG ATGATACCAT CTCAGTTGAA ATGAAGAAGG AAGATTTTGA CATTTATGAT
    GAGGATGAAA ATCAGAGCCC CCGCAGCTTT CAAAAGAAAA CACGACACTA TTTTATTGCT
    GCAGTGGAGA GGCTCTGGGA TTATGGGATG AGTAGCTCCC CACATGTTCT AAGAAACAGG
    GCTCAGAGTG GCAGTGTCCC TCAGTTCAAG AAAGTTGTTT TCCAGGAATT TACTGATGGC
    TCCTTTACTC AGCCCTTATA CCGTGGAGAA CTAAATGAAC ATTTGGGACT CCTGGGGCCA
    TATATAAGAG CAGAAGTTGA AGATAATATC ATGGTAACTT TCAAAAATCA GGCCTCTCGT
    CCCTATTCCT TCTATTCTAG CCTTATTTCT TATGAGGAAG ATCAGAGGCA AGGAGCAGAA
    CCTAGGAAAA ACTTTGTCAA GCCTAATGAA ACCAAAACTT ACTTTTGGAA AGTGCAACAT
    CATATGGCGC CCACTAAAGA TGAGTTTGAC TGCAAAGCCT GGGCTTATTT CTCTGATGTT
    GACCTGGAAA AAGATGTGCA CTCAGGCCTG ATTGGACCCC TTCTGGTCTG CCACACTAAC
    ACACTGAACC CTGCTCATGG GAGACAAGTG ACAGTACAGG AATTTGCTCT GTTTTTCACC
    ATCTTTGATG AGACCAAAAG CTGGTACTTC ACTGAAAACA TGGAAAGAAA CTGCAGGGCT
    CCCTGCAATA TCCAGATGGA AGATCCCACT TTTAAAGAGA ATTATCGCTT CCATGCAATC
    AATGGCTACA TAATGGATAC ACTACCTGGC TTAGTAATGG CTCAGGATCA AAGGATTCGA
    TGGTATCTGC TCAGCATGGG CAGCAATGAA AACATCCATT CTATTCATTT CAGTGGACAT
    GTGTTCACTG TACGAAAAAA AGAGGAGTAT AAAATGGCAC TGTACAATCT CTATCCAGGT
    GTTTTTGAGA CAGTGGAAAT GTTACCATCC AAAGCTGGAA TTTGGCGGGT GGAATGCCTT
    ATTGGCGAGC ATCTACATGC TGGGATGAGC ACACTTTTTC TGGTGTACAG CAATAAGTGT
    CAGACTCCCC TGGGAATGGC TTCTGGACAC ATTAGAGATT TTCAGATTAC AGCTTCAGGA
    CAATATGGAC AGTGGGCCCC AAAGCTGGCC AGACTTCATT ATTCCGGATC AATCAATGCC
    TGGAGCACCA AGGAGCCCTT TTCTTGGATC AAGGTGGATC TGTTGGCACC AATGATTATT
    CACGGCATCA AGACCCAGGG TGCCCGTCAG AAGTTCTCCA GCCTCTACAT CTCTCAGTTT
    ATCATCATGT ATAGTCTTGA TGGGAAGAAG TGGCAGACTT ATCGAGGAAA TTCCACTGGA
    ACCTTAATGG TCTTCTTTGG CAATGTGGAT TCATCTGGGA TAAAACACAA TATTTTTAAC
    CCTCCAATTA TTGCTCGATA CATCCGTTTG CACCCAACTC ATTATAGCAT TCGCAGCACT
    CTTCGCATGG AGTTGATGGG CTGTGATTTA AATAGTTGCA GCATGCCATT GGGAATGGAG
    AGTAAAGCAA TATCAGATGC ACAGATTACT GCTTCATCCT ACTTTACCAA TATGTTTGCC
    ACCTGGTCTC CTTCAAAAGC TCGACTTCAC CTCCAAGGGA GGAGTAATGC CTGGAGACCT
    CAGGTGAATA ATCCAAAAGA GTGGCTGCAA GTGGACTTCC AGAAGACAAT GAAAGTCACA
    GGAATAACTA CTCAGGGAGT AAAATCTCTG CTTACCAGCA TGTATGTGAA GGAGTTCCTC
    ATCTCCAGCA GTCAAGATGG CCATCACTGG ACTCTCTTTT TTCAGAATGG CAAAGTAAAG
    GTTTTTCAGG GAAATCAAGA CTCCTTCACA CCTGTGGTGA ACTCTCTAGA CCCACCGTTA
    CTGACTCGCT ACCTTCGAAT TCACCCCCAG AGTTGGGTGC ACCAGATTGC CCTGAGGATG
    GAGGTTCTGG GCTGCGAGGC ACAGGACCTC TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc25432
    Clone ID Related Accession (Same CDS sequence) XM_003317789.3 , XM_003317789.4
    Accession Version XM_003317789.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7056bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product coagulation factor VIII isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036902.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_003317789.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCAAATAG AGCTCTCCAC CTGCTTCTTT CTGTGCCTTT TGCGATTCTG CTTTAGTGCC 
    ACCAGAAGAT ACTACCTGGG TGCAGTGGAA CTGTCATGGG ACTATATGCA AAGTGATCTC
    GGTGAGCTGC CTGTGGACGC AAGATTTCCT CCTAGAGTGC CAAAATCTTT TCCATTCAAC
    ACCTCAGTCG TGTACAAAAA GACTGTGTTT GTAGAATTCA CGGATCACCT TTTCAACATC
    GCTAAGCCAA GGCCACCCTG GATGGGTCTG CTAGGTCCTA CCATCCAGGC TGAGGTTTAT
    GATACAGTGG TCATTACACT TAAGAACATG GCTTCCCATC CTGTCAGTCT TCATGCTGTT
    GGTGTATCCT ACTGGAAAGC TTCTGAGGGA GCTGAATATG ATGATCAGAC CAGTCAAAGG
    GAGAAAGAAG ATGATAAAGT CTTTCCTGGT GGAAGCCATA CATATGTCTG GCAGGTCCTG
    AAAGAGAATG GTCCAATGGC CTCTGACCCA CTGTGCCTTA CCTACTCATA TCTTTCTCAT
    GTGGACCTGG TCAAAGACTT GAATTCAGGC CTCATTGGAG CCCTACTAGT ATGTAGAGAA
    GGGAGTCTGG CCAAGGAAAA GACACAGACC TTGCACAAAT TTATACTACT TTTTGCTGTA
    TTTGATGAAG GGAAAAGTTG GCACTCAGAA ACAAAGAACT CCTTGATGCA GGATAGGGAT
    GCTGCATCTG CTCGGGCCTG GCCTAAAATG CACACAGTCA ATGGTTATGT AAACAGGTCT
    CTGCCAGGTC TGATTGGATG CCACAGGAAA TCAGTCTATT GGCATGTGAT TGGAATGGGC
    ACCACTCCTG AAGTGCACTC AATATTCCTC GAAGGTCACA CATTTCTTGT GAGGAACCAT
    CGCCAGGCGT CCTTGGAAAT ATCGCCAATA ACTTTCCTTA CTGCTCAAAC ACTCTTGATG
    GACCTTGGAC AGTTTCTACT GTTTTGTCAT ATCTCTTCCC ACCAACATGA TGGCATGGAA
    GCTTATGTCA AAGTAGACAG CTGCCCAGAG GAACCCCAAC TACGAATGAA AAATAATGAA
    GAAGCGGAAG ACTATGATGA TGATCTTACT GATTCTGAAA TGGATGTGGT CAGGTTTGAT
    GATGACAACT CTCCTTCCTT TATCCAAATT CGCTCAGTTG CCAAGAAGCA TCCTAAAACT
    TGGGTACATT ACATTGCTGC TGAAGAGGAG GACTGGGACT ATGCTCCCTT AGTCCTCGCC
    CCCGATGACA GAAGTTATAA AAGTCAATAT TTGAACAATG GCCCTCAGCG GATTGGTAGG
    AAGTACAAAA AAGTCCGATT TATGGCATAC ACAGATGAAA CCTTTAAGAC TCGTGAAGCT
    ATTCAGCATG AATCAGGAAT CTTGGGACCT TTACTTTATG GGGAAGTTGG AGACACACTG
    TTGATTATAT TTAAGAATCA AGCAAGCAGA CCATATAACA TCTACCCTCA CGGAATCACT
    GATGTCCGTC CTTTGTATTC AAGGAGATTA CCAAAAGGTG TAAAACATTT GAAGGATTTT
    CCAATTCTGC CAGGAGAAAT ATTCAAATAT AAATGGACAG TGACTGTAGA AGATGGGCCA
    ACTAAATCAG ATCCTCGGTG CCTGACCCGC TATTACTCTA GTTTCGTTAA TATGGAGAGA
    GATCTAGCTT CAGGACTCAT TGGCCCTCTC CTCATCTGCT ACAAAGAATC TGTAGATCAA
    AGAGGAAACC AGATAATGTC AGACAAGAGG AATGTCATCC TGTTTTCTGT ATTTGATGAG
    AACCGAAGCT GGTACCTCAC AGAGAATATA CAACGCTTTC TCCCCAATCC AGCTGGAGTG
    CAGCTTGAGG ATCCAGAGTT CCAAGCCTCC AACATCATGC ACAGCATCAA TGGCTATGTT
    TTTGATAGTT TGCAGTTGTC AGTTTGTTTG CATGAGGTGG CATACTGGTA CATTCTAAGC
    ATTGGAGCAC AGACTGACTT CCTTTCTGTC TTCTTCTCTG GATATACCTT CAAACACAAA
    ATGGTCTATG AAGATACACT CACCCTATTC CCATTCTCAG GAGAAACTGT CTTCATGTCG
    ATGGAAAACC CAGGCCTATG GATTCTGGGG TGCCACAACT CAGACTTTCG GAACAGAGGC
    ATGACCGCCT TACTGAAGGT TTCTAGTTGT GACAAGAACA CTGGTGATTA TTACGAGGAC
    AGTTATGAAG ATATTTCAGC ATACTTGCTG AGTAAAAACA ATGCCATTGA ACCAAGAAGC
    TTCTCCCAGA ATTCAAGACA CCCTAGCACT AGGCAAAAGC AATTTAATGC CACCACAATT
    CCAGAAAATG ACATAGAGAA GACTGACCCT TGGTTTGCAC ACAGAACACC TATGCCTAAA
    GTACAAAATG TCTCCTCTAG TGATTTGTTG ATGCTCTTGC GACAGAGTCC TACTCCACAT
    GGGCTATCCT TATCTGATCT CCAAGAAGCC AAATATGAGA CTTTTTCTGA TGATCCATCA
    CCTGGAGCAA TAGACAGTAA TAACAGCCTA TCTGAAATGA CACACTTCAG GCCACAGTTC
    CATCACAGTG GGGACATGGT ATTTACCCCT GAGTCAGGCC TCCAATTGAG ATTAAATGAG
    AAACTGGGGA CAACTGCAGC AACAGAGTTG AAGAAACTTG ATTTCAAAGT TTCTAGTACA
    TCAAATAATC TGATTTCAAC AATTCCATCA GACAATTTGG CAGCAGGTAC TGATAATACA
    AGTTCCTTAG GACCCCCAAG TATGCCAGTT CATTATGATA GTCAATTAGA TACCACTCTA
    TTTGGCAAAA AGTCATCTCC CCTTACTGAG TCTGGTAGAC CTCTGAGCTT GAGTGAAGAA
    AATAATGATT CAAAGTTGTT AGAATCAGGT TTAATGAATA GCCAAGAAAG TTCATGGGGA
    AAAAATGTAT CGTCAACAGA GAGTGGTAGG TTATTTAAAG GGAAAAGAGC TCATGGACCT
    GCTTTGTTGA CTAAAGATAA TGCCTTATTC AAAGTTAGCA TCTCTTTGTT AAAGACAAAC
    AAAACTTCCA ATAATTCAGC AACTAATAGA AAGACTCACA TTGATGGCCC ATCATTATTA
    ATTCAGAGTA GTCCATCAGT CTGGCAAAAT ATATTAGAAA GTGACACTGA GTTTAAAAAA
    GTGACACCTT TGATTCATGA CAGAATGCTT ATGGACAAAA ATGCTACAGC TTTGAGGCTA
    AATCATATGT CAAATAAAAC TACTTCATCA AAAAACATGG AAATGGTCCG ACAGAAAAAA
    GAGGGCCCCA TTCCACCAGA TGCAGAAAAT CCAGATATGT CATTCTTTAA GATGCTATTC
    TTGCCAGAAT CAGCAAGTTG GATACAAAGG ACTCATGGAA AGAACTCTCT GAACTCTGGG
    CAAGGCCCCA GTCCAAAGCA ATTAGTATCC TTAGGACCAG AAAAATCTGT GGAAGGTCAG
    AATTTCTTGT CTGAGAAAAA CAAAGTGGTA GTAGGAAAGG GTGAATTTAC AAAGGACGTA
    GGACTCAAAG AGATGGTTTT TCCAAGCAGC AGAAACCTAT TTCTTACTAA CTTGGATAAT
    TTACATGAAA ATAATACACA CAATCAAGAA AAAAAAATTC AGGAAGAAAT AGAAAGGAAG
    GAAACATTAA TCCAAGAGAA TGTAGTTTTG CCTCAGATAC ATACAGTGAC TGGCACTAAG
    AATTTCATGA AGAACCTTTT CTTACTGAGC ACTAGGCAAA ATGTAGAAGG TTCATATGAG
    GGGGCATATG CTCCAGTACT TCAAGATTTT AGGTCATTAA ATGATTCAAC AAATAGAACA
    AAGAAACACA CGGCTCATTT CTCAAAAAAA GGGGAAGAAG AAAACTTGGC AGGCTTGGGA
    AATCAAACCA AGCAAATTGT AGAGAAATAT GCATGCACCA CAAGGATATC TCCTAATACA
    AGCCAGCAGA ATTTTGTCAC GCAACGTAGT AAGAGAGCTT TGAAACAATT CAGACTCCCA
    CTAGAAGAAA CAGAACTTGA AAAAAGGATA ATTGTGGATG ACACCTCAAC CCAGTGGTCC
    AAAAACATGA AACATTTGAC CCCGAGCACC CTCACACAGA TAGACTACAA TGAGAAGGAG
    AAAGGGGCCA TTACTCAGTC TCCCTTATCA GATTGCCTTA CGAGGAGTCA TAGCATCCCT
    CAAGCAAATA GATCTCCATT ACCCATTGCA GAGGTATCAT CATTTCCATC TATTAGACCT
    ATATATCTGA CCAGGGTCCT ATTCCAAGAC AACTCTTCTC ATCTTCCAGC AGCATCTTAT
    AGAAAGAAAG ATTCTGGGGT CCAAGAAAGC AGTCATTTCT TACAAGGAGC CAAAAAAAAT
    AACCTTTCTT TAGCCATTCT AACCTTGGAG ATGACTGGTG ATCAAAGAGA GGTTGGCTCC
    CTGGGGACAA GTGCCACAAA TTCAGTCACT TACAAGAAAG TTGAGAACAC TGTTCTCCCG
    AAACCAGGCT TGCCCAAAAC ATCTGGCAAA GTTGAATTGC TTCCAAAAGT TCACATTTAT
    CAGAAGGACC TATTCCCTAC GGAAACTAGC AATGGGTCTC CTGGCCATCT GGATCTCGTG
    GAAGGGAGCC TTCTTCAGGA AACAGAGGGA GCGATTAAGT GGAATGAAGC AAACAGACCT
    GGAAAAATTC CCTTTCTGAG AGTAGCAACA GAAAGCTCTG CAAAGACTCC CTCCAAGCTA
    TTGGATCCTC TTGCTTGGGA TAACCACTAT GGTACTCAGA TACCAAAAGA AGAGTGGAAA
    TCCCAAGAGA AGTCACCAGA AAAAACAGCT TTTAAGAAAA AGGATACCAT TTTGTCCCTG
    AACGCTTGTG AAAGCAATCA TGCAATAGCA GCAATAAATG AGGGACAAAA TAAGCCCGAA
    ATAGAAGTCA CCTGGGCAAA GCAAGGTAGG ACTGAAAGGC TGTGCTCTCA AAACCCACCA
    GGCTTGAAAC GCCATCAACG GGAAATAACT CGTACTACTC TTCAGTCAGA TCAAGAGGAA
    ATTGACTATG ATGATACCAT CTCAGTTGAA ATGAAGAAGG AAGATTTTGA CATTTATGAT
    GAGGATGAAA ATCAGAGCCC CCGCAGCTTT CAAAAGAAAA CACGACACTA TTTTATTGCT
    GCAGTGGAGA GGCTCTGGGA TTATGGGATG AGTAGCTCCC CACATGTTCT AAGAAACAGG
    GCTCAGAGTG GCAGTGTCCC TCAGTTCAAG AAAGTTGTTT TCCAGGAATT TACTGATGGC
    TCCTTTACTC AGCCCTTATA CCGTGGAGAA CTAAATGAAC ATTTGGGACT CCTGGGGCCA
    TATATAAGAG CAGAAGTTGA AGATAATATC ATGGTAACTT TCAAAAATCA GGCCTCTCGT
    CCCTATTCCT TCTATTCTAG CCTTATTTCT TATGAGGAAG ATCAGAGGCA AGGAGCAGAA
    CCTAGGAAAA ACTTTGTCAA GCCTAATGAA ACCAAAACTT ACTTTTGGAA AGTGCAACAT
    CATATGGCGC CCACTAAAGA TGAGTTTGAC TGCAAAGCCT GGGCTTATTT CTCTGATGTT
    GACCTGGAAA AAGATGTGCA CTCAGGCCTG ATTGGACCCC TTCTGGTCTG CCACACTAAC
    ACACTGAACC CTGCTCATGG GAGACAAGTG ACAGTACAGG AATTTGCTCT GTTTTTCACC
    ATCTTTGATG AGACCAAAAG CTGGTACTTC ACTGAAAACA TGGAAAGAAA CTGCAGGGCT
    CCCTGCAATA TCCAGATGGA AGATCCCACT TTTAAAGAGA ATTATCGCTT CCATGCAATC
    AATGGCTACA TAATGGATAC ACTACCTGGC TTAGTAATGG CTCAGGATCA AAGGATTCGA
    TGGTATCTGC TCAGCATGGG CAGCAATGAA AACATCCATT CTATTCATTT CAGTGGACAT
    GTGTTCACTG TACGAAAAAA AGAGGAGTAT AAAATGGCAC TGTACAATCT CTATCCAGGT
    GTTTTTGAGA CAGTGGAAAT GTTACCATCC AAAGCTGGAA TTTGGCGGGT GGAATGCCTT
    ATTGGCGAGC ATCTACATGC TGGGATGAGC ACACTTTTTC TGGTGTACAG CAATAAGTGT
    CAGACTCCCC TGGGAATGGC TTCTGGACAC ATTAGAGATT TTCAGATTAC AGCTTCAGGA
    CAATATGGAC AGTGGGCCCC AAAGCTGGCC AGACTTCATT ATTCCGGATC AATCAATGCC
    TGGAGCACCA AGGAGCCCTT TTCTTGGATC AAGGTGGATC TGTTGGCACC AATGATTATT
    CACGGCATCA AGACCCAGGG TGCCCGTCAG AAGTTCTCCA GCCTCTACAT CTCTCAGTTT
    ATCATCATGT ATAGTCTTGA TGGGAAGAAG TGGCAGACTT ATCGAGGAAA TTCCACTGGA
    ACCTTAATGG TCTTCTTTGG CAATGTGGAT TCATCTGGGA TAAAACACAA TATTTTTAAC
    CCTCCAATTA TTGCTCGATA CATCCGTTTG CACCCAACTC ATTATAGCAT TCGCAGCACT
    CTTCGCATGG AGTTGATGGG CTGTGATTTA AATAGTTGCA GCATGCCATT GGGAATGGAG
    AGTAAAGCAA TATCAGATGC ACAGATTACT GCTTCATCCT ACTTTACCAA TATGTTTGCC
    ACCTGGTCTC CTTCAAAAGC TCGACTTCAC CTCCAAGGGA GGAGTAATGC CTGGAGACCT
    CAGGTGAATA ATCCAAAAGA GTGGCTGCAA GTGGACTTCC AGAAGACAAT GAAAGTCACA
    GGAATAACTA CTCAGGGAGT AAAATCTCTG CTTACCAGCA TGTATGTGAA GGAGTTCCTC
    ATCTCCAGCA GTCAAGATGG CCATCACTGG ACTCTCTTTT TTCAGAATGG CAAAGTAAAG
    GTTTTTCAGG GAAATCAAGA CTCCTTCACA CCTGTGGTGA ACTCTCTAGA CCCACCGTTA
    CTGACTCGCT ACCTTCGAAT TCACCCCCAG AGTTGGGTGC ACCAGATTGC CCTGAGGATG
    GAGGTTCTGG GCTGCGAGGC ACAGGACCTC TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003317837.1
    CDS400..7455
    Translation

    Target ORF information:

    RefSeq Version XM_003317789.4
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes coagulation factor VIII (F8), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003317789.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGCAAATAG AGCTCTCCAC CTGCTTCTTT CTGTGCCTTT TGCGATTCTG CTTTAGTGCC 
    ACCAGAAGAT ACTACCTGGG TGCAGTGGAA CTGTCATGGG ACTATATGCA AAGTGATCTC
    GGTGAGCTGC CTGTGGACGC AAGATTTCCT CCTAGAGTGC CAAAATCTTT TCCATTCAAC
    ACCTCAGTCG TGTACAAAAA GACTGTGTTT GTAGAATTCA CGGATCACCT TTTCAACATC
    GCTAAGCCAA GGCCACCCTG GATGGGTCTG CTAGGTCCTA CCATCCAGGC TGAGGTTTAT
    GATACAGTGG TCATTACACT TAAGAACATG GCTTCCCATC CTGTCAGTCT TCATGCTGTT
    GGTGTATCCT ACTGGAAAGC TTCTGAGGGA GCTGAATATG ATGATCAGAC CAGTCAAAGG
    GAGAAAGAAG ATGATAAAGT CTTTCCTGGT GGAAGCCATA CATATGTCTG GCAGGTCCTG
    AAAGAGAATG GTCCAATGGC CTCTGACCCA CTGTGCCTTA CCTACTCATA TCTTTCTCAT
    GTGGACCTGG TCAAAGACTT GAATTCAGGC CTCATTGGAG CCCTACTAGT ATGTAGAGAA
    GGGAGTCTGG CCAAGGAAAA GACACAGACC TTGCACAAAT TTATACTACT TTTTGCTGTA
    TTTGATGAAG GGAAAAGTTG GCACTCAGAA ACAAAGAACT CCTTGATGCA GGATAGGGAT
    GCTGCATCTG CTCGGGCCTG GCCTAAAATG CACACAGTCA ATGGTTATGT AAACAGGTCT
    CTGCCAGGTC TGATTGGATG CCACAGGAAA TCAGTCTATT GGCATGTGAT TGGAATGGGC
    ACCACTCCTG AAGTGCACTC AATATTCCTC GAAGGTCACA CATTTCTTGT GAGGAACCAT
    CGCCAGGCGT CCTTGGAAAT ATCGCCAATA ACTTTCCTTA CTGCTCAAAC ACTCTTGATG
    GACCTTGGAC AGTTTCTACT GTTTTGTCAT ATCTCTTCCC ACCAACATGA TGGCATGGAA
    GCTTATGTCA AAGTAGACAG CTGCCCAGAG GAACCCCAAC TACGAATGAA AAATAATGAA
    GAAGCGGAAG ACTATGATGA TGATCTTACT GATTCTGAAA TGGATGTGGT CAGGTTTGAT
    GATGACAACT CTCCTTCCTT TATCCAAATT CGCTCAGTTG CCAAGAAGCA TCCTAAAACT
    TGGGTACATT ACATTGCTGC TGAAGAGGAG GACTGGGACT ATGCTCCCTT AGTCCTCGCC
    CCCGATGACA GAAGTTATAA AAGTCAATAT TTGAACAATG GCCCTCAGCG GATTGGTAGG
    AAGTACAAAA AAGTCCGATT TATGGCATAC ACAGATGAAA CCTTTAAGAC TCGTGAAGCT
    ATTCAGCATG AATCAGGAAT CTTGGGACCT TTACTTTATG GGGAAGTTGG AGACACACTG
    TTGATTATAT TTAAGAATCA AGCAAGCAGA CCATATAACA TCTACCCTCA CGGAATCACT
    GATGTCCGTC CTTTGTATTC AAGGAGATTA CCAAAAGGTG TAAAACATTT GAAGGATTTT
    CCAATTCTGC CAGGAGAAAT ATTCAAATAT AAATGGACAG TGACTGTAGA AGATGGGCCA
    ACTAAATCAG ATCCTCGGTG CCTGACCCGC TATTACTCTA GTTTCGTTAA TATGGAGAGA
    GATCTAGCTT CAGGACTCAT TGGCCCTCTC CTCATCTGCT ACAAAGAATC TGTAGATCAA
    AGAGGAAACC AGATAATGTC AGACAAGAGG AATGTCATCC TGTTTTCTGT ATTTGATGAG
    AACCGAAGCT GGTACCTCAC AGAGAATATA CAACGCTTTC TCCCCAATCC AGCTGGAGTG
    CAGCTTGAGG ATCCAGAGTT CCAAGCCTCC AACATCATGC ACAGCATCAA TGGCTATGTT
    TTTGATAGTT TGCAGTTGTC AGTTTGTTTG CATGAGGTGG CATACTGGTA CATTCTAAGC
    ATTGGAGCAC AGACTGACTT CCTTTCTGTC TTCTTCTCTG GATATACCTT CAAACACAAA
    ATGGTCTATG AAGATACACT CACCCTATTC CCATTCTCAG GAGAAACTGT CTTCATGTCG
    ATGGAAAACC CAGGCCTATG GATTCTGGGG TGCCACAACT CAGACTTTCG GAACAGAGGC
    ATGACCGCCT TACTGAAGGT TTCTAGTTGT GACAAGAACA CTGGTGATTA TTACGAGGAC
    AGTTATGAAG ATATTTCAGC ATACTTGCTG AGTAAAAACA ATGCCATTGA ACCAAGAAGC
    TTCTCCCAGA ATTCAAGACA CCCTAGCACT AGGCAAAAGC AATTTAATGC CACCACAATT
    CCAGAAAATG ACATAGAGAA GACTGACCCT TGGTTTGCAC ACAGAACACC TATGCCTAAA
    GTACAAAATG TCTCCTCTAG TGATTTGTTG ATGCTCTTGC GACAGAGTCC TACTCCACAT
    GGGCTATCCT TATCTGATCT CCAAGAAGCC AAATATGAGA CTTTTTCTGA TGATCCATCA
    CCTGGAGCAA TAGACAGTAA TAACAGCCTA TCTGAAATGA CACACTTCAG GCCACAGTTC
    CATCACAGTG GGGACATGGT ATTTACCCCT GAGTCAGGCC TCCAATTGAG ATTAAATGAG
    AAACTGGGGA CAACTGCAGC AACAGAGTTG AAGAAACTTG ATTTCAAAGT TTCTAGTACA
    TCAAATAATC TGATTTCAAC AATTCCATCA GACAATTTGG CAGCAGGTAC TGATAATACA
    AGTTCCTTAG GACCCCCAAG TATGCCAGTT CATTATGATA GTCAATTAGA TACCACTCTA
    TTTGGCAAAA AGTCATCTCC CCTTACTGAG TCTGGTAGAC CTCTGAGCTT GAGTGAAGAA
    AATAATGATT CAAAGTTGTT AGAATCAGGT TTAATGAATA GCCAAGAAAG TTCATGGGGA
    AAAAATGTAT CGTCAACAGA GAGTGGTAGG TTATTTAAAG GGAAAAGAGC TCATGGACCT
    GCTTTGTTGA CTAAAGATAA TGCCTTATTC AAAGTTAGCA TCTCTTTGTT AAAGACAAAC
    AAAACTTCCA ATAATTCAGC AACTAATAGA AAGACTCACA TTGATGGCCC ATCATTATTA
    ATTCAGAGTA GTCCATCAGT CTGGCAAAAT ATATTAGAAA GTGACACTGA GTTTAAAAAA
    GTGACACCTT TGATTCATGA CAGAATGCTT ATGGACAAAA ATGCTACAGC TTTGAGGCTA
    AATCATATGT CAAATAAAAC TACTTCATCA AAAAACATGG AAATGGTCCG ACAGAAAAAA
    GAGGGCCCCA TTCCACCAGA TGCAGAAAAT CCAGATATGT CATTCTTTAA GATGCTATTC
    TTGCCAGAAT CAGCAAGTTG GATACAAAGG ACTCATGGAA AGAACTCTCT GAACTCTGGG
    CAAGGCCCCA GTCCAAAGCA ATTAGTATCC TTAGGACCAG AAAAATCTGT GGAAGGTCAG
    AATTTCTTGT CTGAGAAAAA CAAAGTGGTA GTAGGAAAGG GTGAATTTAC AAAGGACGTA
    GGACTCAAAG AGATGGTTTT TCCAAGCAGC AGAAACCTAT TTCTTACTAA CTTGGATAAT
    TTACATGAAA ATAATACACA CAATCAAGAA AAAAAAATTC AGGAAGAAAT AGAAAGGAAG
    GAAACATTAA TCCAAGAGAA TGTAGTTTTG CCTCAGATAC ATACAGTGAC TGGCACTAAG
    AATTTCATGA AGAACCTTTT CTTACTGAGC ACTAGGCAAA ATGTAGAAGG TTCATATGAG
    GGGGCATATG CTCCAGTACT TCAAGATTTT AGGTCATTAA ATGATTCAAC AAATAGAACA
    AAGAAACACA CGGCTCATTT CTCAAAAAAA GGGGAAGAAG AAAACTTGGC AGGCTTGGGA
    AATCAAACCA AGCAAATTGT AGAGAAATAT GCATGCACCA CAAGGATATC TCCTAATACA
    AGCCAGCAGA ATTTTGTCAC GCAACGTAGT AAGAGAGCTT TGAAACAATT CAGACTCCCA
    CTAGAAGAAA CAGAACTTGA AAAAAGGATA ATTGTGGATG ACACCTCAAC CCAGTGGTCC
    AAAAACATGA AACATTTGAC CCCGAGCACC CTCACACAGA TAGACTACAA TGAGAAGGAG
    AAAGGGGCCA TTACTCAGTC TCCCTTATCA GATTGCCTTA CGAGGAGTCA TAGCATCCCT
    CAAGCAAATA GATCTCCATT ACCCATTGCA GAGGTATCAT CATTTCCATC TATTAGACCT
    ATATATCTGA CCAGGGTCCT ATTCCAAGAC AACTCTTCTC ATCTTCCAGC AGCATCTTAT
    AGAAAGAAAG ATTCTGGGGT CCAAGAAAGC AGTCATTTCT TACAAGGAGC CAAAAAAAAT
    AACCTTTCTT TAGCCATTCT AACCTTGGAG ATGACTGGTG ATCAAAGAGA GGTTGGCTCC
    CTGGGGACAA GTGCCACAAA TTCAGTCACT TACAAGAAAG TTGAGAACAC TGTTCTCCCG
    AAACCAGGCT TGCCCAAAAC ATCTGGCAAA GTTGAATTGC TTCCAAAAGT TCACATTTAT
    CAGAAGGACC TATTCCCTAC GGAAACTAGC AATGGGTCTC CTGGCCATCT GGATCTCGTG
    GAAGGGAGCC TTCTTCAGGA AACAGAGGGA GCGATTAAGT GGAATGAAGC AAACAGACCT
    GGAAAAATTC CCTTTCTGAG AGTAGCAACA GAAAGCTCTG CAAAGACTCC CTCCAAGCTA
    TTGGATCCTC TTGCTTGGGA TAACCACTAT GGTACTCAGA TACCAAAAGA AGAGTGGAAA
    TCCCAAGAGA AGTCACCAGA AAAAACAGCT TTTAAGAAAA AGGATACCAT TTTGTCCCTG
    AACGCTTGTG AAAGCAATCA TGCAATAGCA GCAATAAATG AGGGACAAAA TAAGCCCGAA
    ATAGAAGTCA CCTGGGCAAA GCAAGGTAGG ACTGAAAGGC TGTGCTCTCA AAACCCACCA
    GGCTTGAAAC GCCATCAACG GGAAATAACT CGTACTACTC TTCAGTCAGA TCAAGAGGAA
    ATTGACTATG ATGATACCAT CTCAGTTGAA ATGAAGAAGG AAGATTTTGA CATTTATGAT
    GAGGATGAAA ATCAGAGCCC CCGCAGCTTT CAAAAGAAAA CACGACACTA TTTTATTGCT
    GCAGTGGAGA GGCTCTGGGA TTATGGGATG AGTAGCTCCC CACATGTTCT AAGAAACAGG
    GCTCAGAGTG GCAGTGTCCC TCAGTTCAAG AAAGTTGTTT TCCAGGAATT TACTGATGGC
    TCCTTTACTC AGCCCTTATA CCGTGGAGAA CTAAATGAAC ATTTGGGACT CCTGGGGCCA
    TATATAAGAG CAGAAGTTGA AGATAATATC ATGGTAACTT TCAAAAATCA GGCCTCTCGT
    CCCTATTCCT TCTATTCTAG CCTTATTTCT TATGAGGAAG ATCAGAGGCA AGGAGCAGAA
    CCTAGGAAAA ACTTTGTCAA GCCTAATGAA ACCAAAACTT ACTTTTGGAA AGTGCAACAT
    CATATGGCGC CCACTAAAGA TGAGTTTGAC TGCAAAGCCT GGGCTTATTT CTCTGATGTT
    GACCTGGAAA AAGATGTGCA CTCAGGCCTG ATTGGACCCC TTCTGGTCTG CCACACTAAC
    ACACTGAACC CTGCTCATGG GAGACAAGTG ACAGTACAGG AATTTGCTCT GTTTTTCACC
    ATCTTTGATG AGACCAAAAG CTGGTACTTC ACTGAAAACA TGGAAAGAAA CTGCAGGGCT
    CCCTGCAATA TCCAGATGGA AGATCCCACT TTTAAAGAGA ATTATCGCTT CCATGCAATC
    AATGGCTACA TAATGGATAC ACTACCTGGC TTAGTAATGG CTCAGGATCA AAGGATTCGA
    TGGTATCTGC TCAGCATGGG CAGCAATGAA AACATCCATT CTATTCATTT CAGTGGACAT
    GTGTTCACTG TACGAAAAAA AGAGGAGTAT AAAATGGCAC TGTACAATCT CTATCCAGGT
    GTTTTTGAGA CAGTGGAAAT GTTACCATCC AAAGCTGGAA TTTGGCGGGT GGAATGCCTT
    ATTGGCGAGC ATCTACATGC TGGGATGAGC ACACTTTTTC TGGTGTACAG CAATAAGTGT
    CAGACTCCCC TGGGAATGGC TTCTGGACAC ATTAGAGATT TTCAGATTAC AGCTTCAGGA
    CAATATGGAC AGTGGGCCCC AAAGCTGGCC AGACTTCATT ATTCCGGATC AATCAATGCC
    TGGAGCACCA AGGAGCCCTT TTCTTGGATC AAGGTGGATC TGTTGGCACC AATGATTATT
    CACGGCATCA AGACCCAGGG TGCCCGTCAG AAGTTCTCCA GCCTCTACAT CTCTCAGTTT
    ATCATCATGT ATAGTCTTGA TGGGAAGAAG TGGCAGACTT ATCGAGGAAA TTCCACTGGA
    ACCTTAATGG TCTTCTTTGG CAATGTGGAT TCATCTGGGA TAAAACACAA TATTTTTAAC
    CCTCCAATTA TTGCTCGATA CATCCGTTTG CACCCAACTC ATTATAGCAT TCGCAGCACT
    CTTCGCATGG AGTTGATGGG CTGTGATTTA AATAGTTGCA GCATGCCATT GGGAATGGAG
    AGTAAAGCAA TATCAGATGC ACAGATTACT GCTTCATCCT ACTTTACCAA TATGTTTGCC
    ACCTGGTCTC CTTCAAAAGC TCGACTTCAC CTCCAAGGGA GGAGTAATGC CTGGAGACCT
    CAGGTGAATA ATCCAAAAGA GTGGCTGCAA GTGGACTTCC AGAAGACAAT GAAAGTCACA
    GGAATAACTA CTCAGGGAGT AAAATCTCTG CTTACCAGCA TGTATGTGAA GGAGTTCCTC
    ATCTCCAGCA GTCAAGATGG CCATCACTGG ACTCTCTTTT TTCAGAATGG CAAAGTAAAG
    GTTTTTCAGG GAAATCAAGA CTCCTTCACA CCTGTGGTGA ACTCTCTAGA CCCACCGTTA
    CTGACTCGCT ACCTTCGAAT TCACCCCCAG AGTTGGGTGC ACCAGATTGC CCTGAGGATG
    GAGGTTCTGG GCTGCGAGGC ACAGGACCTC TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc46412
    Clone ID Related Accession (Same CDS sequence) XM_009439870.2
    Accession Version XM_009439870.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6351bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product coagulation factor VIII isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036902.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009439870.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCAGGATA GGGATGCTGC ATCTGCTCGG GCCTGGCCTA AAATGCACAC AGTCAATGGT 
    TATGTAAACA GGTCTCTGCC AGGTCTGATT GGATGCCACA GGAAATCAGT CTATTGGCAT
    GTGATTGGAA TGGGCACCAC TCCTGAAGTG CACTCAATAT TCCTCGAAGG TCACACATTT
    CTTGTGAGGA ACCATCGCCA GGCGTCCTTG GAAATATCGC CAATAACTTT CCTTACTGCT
    CAAACACTCT TGATGGACCT TGGACAGTTT CTACTGTTTT GTCATATCTC TTCCCACCAA
    CATGATGGCA TGGAAGCTTA TGTCAAAGTA GACAGCTGCC CAGAGGAACC CCAACTACGA
    ATGAAAAATA ATGAAGAAGC GGAAGACTAT GATGATGATC TTACTGATTC TGAAATGGAT
    GTGGTCAGGT TTGATGATGA CAACTCTCCT TCCTTTATCC AAATTCGCTC AGTTGCCAAG
    AAGCATCCTA AAACTTGGGT ACATTACATT GCTGCTGAAG AGGAGGACTG GGACTATGCT
    CCCTTAGTCC TCGCCCCCGA TGACAGAAGT TATAAAAGTC AATATTTGAA CAATGGCCCT
    CAGCGGATTG GTAGGAAGTA CAAAAAAGTC CGATTTATGG CATACACAGA TGAAACCTTT
    AAGACTCGTG AAGCTATTCA GCATGAATCA GGAATCTTGG GACCTTTACT TTATGGGGAA
    GTTGGAGACA CACTGTTGAT TATATTTAAG AATCAAGCAA GCAGACCATA TAACATCTAC
    CCTCACGGAA TCACTGATGT CCGTCCTTTG TATTCAAGGA GATTACCAAA AGGTGTAAAA
    CATTTGAAGG ATTTTCCAAT TCTGCCAGGA GAAATATTCA AATATAAATG GACAGTGACT
    GTAGAAGATG GGCCAACTAA ATCAGATCCT CGGTGCCTGA CCCGCTATTA CTCTAGTTTC
    GTTAATATGG AGAGAGATCT AGCTTCAGGA CTCATTGGCC CTCTCCTCAT CTGCTACAAA
    GAATCTGTAG ATCAAAGAGG AAACCAGATA ATGTCAGACA AGAGGAATGT CATCCTGTTT
    TCTGTATTTG ATGAGAACCG AAGCTGGTAC CTCACAGAGA ATATACAACG CTTTCTCCCC
    AATCCAGCTG GAGTGCAGCT TGAGGATCCA GAGTTCCAAG CCTCCAACAT CATGCACAGC
    ATCAATGGCT ATGTTTTTGA TAGTTTGCAG TTGTCAGTTT GTTTGCATGA GGTGGCATAC
    TGGTACATTC TAAGCATTGG AGCACAGACT GACTTCCTTT CTGTCTTCTT CTCTGGATAT
    ACCTTCAAAC ACAAAATGGT CTATGAAGAT ACACTCACCC TATTCCCATT CTCAGGAGAA
    ACTGTCTTCA TGTCGATGGA AAACCCAGGC CTATGGATTC TGGGGTGCCA CAACTCAGAC
    TTTCGGAACA GAGGCATGAC CGCCTTACTG AAGGTTTCTA GTTGTGACAA GAACACTGGT
    GATTATTACG AGGACAGTTA TGAAGATATT TCAGCATACT TGCTGAGTAA AAACAATGCC
    ATTGAACCAA GAAGCTTCTC CCAGAATTCA AGACACCCTA GCACTAGGCA AAAGCAATTT
    AATGCCACCA CAATTCCAGA AAATGACATA GAGAAGACTG ACCCTTGGTT TGCACACAGA
    ACACCTATGC CTAAAGTACA AAATGTCTCC TCTAGTGATT TGTTGATGCT CTTGCGACAG
    AGTCCTACTC CACATGGGCT ATCCTTATCT GATCTCCAAG AAGCCAAATA TGAGACTTTT
    TCTGATGATC CATCACCTGG AGCAATAGAC AGTAATAACA GCCTATCTGA AATGACACAC
    TTCAGGCCAC AGTTCCATCA CAGTGGGGAC ATGGTATTTA CCCCTGAGTC AGGCCTCCAA
    TTGAGATTAA ATGAGAAACT GGGGACAACT GCAGCAACAG AGTTGAAGAA ACTTGATTTC
    AAAGTTTCTA GTACATCAAA TAATCTGATT TCAACAATTC CATCAGACAA TTTGGCAGCA
    GGTACTGATA ATACAAGTTC CTTAGGACCC CCAAGTATGC CAGTTCATTA TGATAGTCAA
    TTAGATACCA CTCTATTTGG CAAAAAGTCA TCTCCCCTTA CTGAGTCTGG TAGACCTCTG
    AGCTTGAGTG AAGAAAATAA TGATTCAAAG TTGTTAGAAT CAGGTTTAAT GAATAGCCAA
    GAAAGTTCAT GGGGAAAAAA TGTATCGTCA ACAGAGAGTG GTAGGTTATT TAAAGGGAAA
    AGAGCTCATG GACCTGCTTT GTTGACTAAA GATAATGCCT TATTCAAAGT TAGCATCTCT
    TTGTTAAAGA CAAACAAAAC TTCCAATAAT TCAGCAACTA ATAGAAAGAC TCACATTGAT
    GGCCCATCAT TATTAATTCA GAGTAGTCCA TCAGTCTGGC AAAATATATT AGAAAGTGAC
    ACTGAGTTTA AAAAAGTGAC ACCTTTGATT CATGACAGAA TGCTTATGGA CAAAAATGCT
    ACAGCTTTGA GGCTAAATCA TATGTCAAAT AAAACTACTT CATCAAAAAA CATGGAAATG
    GTCCGACAGA AAAAAGAGGG CCCCATTCCA CCAGATGCAG AAAATCCAGA TATGTCATTC
    TTTAAGATGC TATTCTTGCC AGAATCAGCA AGTTGGATAC AAAGGACTCA TGGAAAGAAC
    TCTCTGAACT CTGGGCAAGG CCCCAGTCCA AAGCAATTAG TATCCTTAGG ACCAGAAAAA
    TCTGTGGAAG GTCAGAATTT CTTGTCTGAG AAAAACAAAG TGGTAGTAGG AAAGGGTGAA
    TTTACAAAGG ACGTAGGACT CAAAGAGATG GTTTTTCCAA GCAGCAGAAA CCTATTTCTT
    ACTAACTTGG ATAATTTACA TGAAAATAAT ACACACAATC AAGAAAAAAA AATTCAGGAA
    GAAATAGAAA GGAAGGAAAC ATTAATCCAA GAGAATGTAG TTTTGCCTCA GATACATACA
    GTGACTGGCA CTAAGAATTT CATGAAGAAC CTTTTCTTAC TGAGCACTAG GCAAAATGTA
    GAAGGTTCAT ATGAGGGGGC ATATGCTCCA GTACTTCAAG ATTTTAGGTC ATTAAATGAT
    TCAACAAATA GAACAAAGAA ACACACGGCT CATTTCTCAA AAAAAGGGGA AGAAGAAAAC
    TTGGCAGGCT TGGGAAATCA AACCAAGCAA ATTGTAGAGA AATATGCATG CACCACAAGG
    ATATCTCCTA ATACAAGCCA GCAGAATTTT GTCACGCAAC GTAGTAAGAG AGCTTTGAAA
    CAATTCAGAC TCCCACTAGA AGAAACAGAA CTTGAAAAAA GGATAATTGT GGATGACACC
    TCAACCCAGT GGTCCAAAAA CATGAAACAT TTGACCCCGA GCACCCTCAC ACAGATAGAC
    TACAATGAGA AGGAGAAAGG GGCCATTACT CAGTCTCCCT TATCAGATTG CCTTACGAGG
    AGTCATAGCA TCCCTCAAGC AAATAGATCT CCATTACCCA TTGCAGAGGT ATCATCATTT
    CCATCTATTA GACCTATATA TCTGACCAGG GTCCTATTCC AAGACAACTC TTCTCATCTT
    CCAGCAGCAT CTTATAGAAA GAAAGATTCT GGGGTCCAAG AAAGCAGTCA TTTCTTACAA
    GGAGCCAAAA AAAATAACCT TTCTTTAGCC ATTCTAACCT TGGAGATGAC TGGTGATCAA
    AGAGAGGTTG GCTCCCTGGG GACAAGTGCC ACAAATTCAG TCACTTACAA GAAAGTTGAG
    AACACTGTTC TCCCGAAACC AGGCTTGCCC AAAACATCTG GCAAAGTTGA ATTGCTTCCA
    AAAGTTCACA TTTATCAGAA GGACCTATTC CCTACGGAAA CTAGCAATGG GTCTCCTGGC
    CATCTGGATC TCGTGGAAGG GAGCCTTCTT CAGGAAACAG AGGGAGCGAT TAAGTGGAAT
    GAAGCAAACA GACCTGGAAA AATTCCCTTT CTGAGAGTAG CAACAGAAAG CTCTGCAAAG
    ACTCCCTCCA AGCTATTGGA TCCTCTTGCT TGGGATAACC ACTATGGTAC TCAGATACCA
    AAAGAAGAGT GGAAATCCCA AGAGAAGTCA CCAGAAAAAA CAGCTTTTAA GAAAAAGGAT
    ACCATTTTGT CCCTGAACGC TTGTGAAAGC AATCATGCAA TAGCAGCAAT AAATGAGGGA
    CAAAATAAGC CCGAAATAGA AGTCACCTGG GCAAAGCAAG GTAGGACTGA AAGGCTGTGC
    TCTCAAAACC CACCAGGCTT GAAACGCCAT CAACGGGAAA TAACTCGTAC TACTCTTCAG
    TCAGATCAAG AGGAAATTGA CTATGATGAT ACCATCTCAG TTGAAATGAA GAAGGAAGAT
    TTTGACATTT ATGATGAGGA TGAAAATCAG AGCCCCCGCA GCTTTCAAAA GAAAACACGA
    CACTATTTTA TTGCTGCAGT GGAGAGGCTC TGGGATTATG GGATGAGTAG CTCCCCACAT
    GTTCTAAGAA ACAGGGCTCA GAGTGGCAGT GTCCCTCAGT TCAAGAAAGT TGTTTTCCAG
    GAATTTACTG ATGGCTCCTT TACTCAGCCC TTATACCGTG GAGAACTAAA TGAACATTTG
    GGACTCCTGG GGCCATATAT AAGAGCAGAA GTTGAAGATA ATATCATGGT AACTTTCAAA
    AATCAGGCCT CTCGTCCCTA TTCCTTCTAT TCTAGCCTTA TTTCTTATGA GGAAGATCAG
    AGGCAAGGAG CAGAACCTAG GAAAAACTTT GTCAAGCCTA ATGAAACCAA AACTTACTTT
    TGGAAAGTGC AACATCATAT GGCGCCCACT AAAGATGAGT TTGACTGCAA AGCCTGGGCT
    TATTTCTCTG ATGTTGACCT GGAAAAAGAT GTGCACTCAG GCCTGATTGG ACCCCTTCTG
    GTCTGCCACA CTAACACACT GAACCCTGCT CATGGGAGAC AAGTGACAGT ACAGGAATTT
    GCTCTGTTTT TCACCATCTT TGATGAGACC AAAAGCTGGT ACTTCACTGA AAACATGGAA
    AGAAACTGCA GGGCTCCCTG CAATATCCAG ATGGAAGATC CCACTTTTAA AGAGAATTAT
    CGCTTCCATG CAATCAATGG CTACATAATG GATACACTAC CTGGCTTAGT AATGGCTCAG
    GATCAAAGGA TTCGATGGTA TCTGCTCAGC ATGGGCAGCA ATGAAAACAT CCATTCTATT
    CATTTCAGTG GACATGTGTT CACTGTACGA AAAAAAGAGG AGTATAAAAT GGCACTGTAC
    AATCTCTATC CAGGTGTTTT TGAGACAGTG GAAATGTTAC CATCCAAAGC TGGAATTTGG
    CGGGTGGAAT GCCTTATTGG CGAGCATCTA CATGCTGGGA TGAGCACACT TTTTCTGGTG
    TACAGCAATA AGTGTCAGAC TCCCCTGGGA ATGGCTTCTG GACACATTAG AGATTTTCAG
    ATTACAGCTT CAGGACAATA TGGACAGTGG GCCCCAAAGC TGGCCAGACT TCATTATTCC
    GGATCAATCA ATGCCTGGAG CACCAAGGAG CCCTTTTCTT GGATCAAGGT GGATCTGTTG
    GCACCAATGA TTATTCACGG CATCAAGACC CAGGGTGCCC GTCAGAAGTT CTCCAGCCTC
    TACATCTCTC AGTTTATCAT CATGTATAGT CTTGATGGGA AGAAGTGGCA GACTTATCGA
    GGAAATTCCA CTGGAACCTT AATGGTCTTC TTTGGCAATG TGGATTCATC TGGGATAAAA
    CACAATATTT TTAACCCTCC AATTATTGCT CGATACATCC GTTTGCACCC AACTCATTAT
    AGCATTCGCA GCACTCTTCG CATGGAGTTG ATGGGCTGTG ATTTAAATAG TTGCAGCATG
    CCATTGGGAA TGGAGAGTAA AGCAATATCA GATGCACAGA TTACTGCTTC ATCCTACTTT
    ACCAATATGT TTGCCACCTG GTCTCCTTCA AAAGCTCGAC TTCACCTCCA AGGGAGGAGT
    AATGCCTGGA GACCTCAGGT GAATAATCCA AAAGAGTGGC TGCAAGTGGA CTTCCAGAAG
    ACAATGAAAG TCACAGGAAT AACTACTCAG GGAGTAAAAT CTCTGCTTAC CAGCATGTAT
    GTGAAGGAGT TCCTCATCTC CAGCAGTCAA GATGGCCATC ACTGGACTCT CTTTTTTCAG
    AATGGCAAAG TAAAGGTTTT TCAGGGAAAT CAAGACTCCT TCACACCTGT GGTGAACTCT
    CTAGACCCAC CGTTACTGAC TCGCTACCTT CGAATTCACC CCCAGAGTTG GGTGCACCAG
    ATTGCCCTGA GGATGGAGGT TCTGGGCTGC GAGGCACAGG ACCTCTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009438145.1
    CDS206..6556
    Translation

    Target ORF information:

    RefSeq Version XM_009439870.2
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes coagulation factor VIII (F8), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009439870.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGCAGGATA GGGATGCTGC ATCTGCTCGG GCCTGGCCTA AAATGCACAC AGTCAATGGT 
    TATGTAAACA GGTCTCTGCC AGGTCTGATT GGATGCCACA GGAAATCAGT CTATTGGCAT
    GTGATTGGAA TGGGCACCAC TCCTGAAGTG CACTCAATAT TCCTCGAAGG TCACACATTT
    CTTGTGAGGA ACCATCGCCA GGCGTCCTTG GAAATATCGC CAATAACTTT CCTTACTGCT
    CAAACACTCT TGATGGACCT TGGACAGTTT CTACTGTTTT GTCATATCTC TTCCCACCAA
    CATGATGGCA TGGAAGCTTA TGTCAAAGTA GACAGCTGCC CAGAGGAACC CCAACTACGA
    ATGAAAAATA ATGAAGAAGC GGAAGACTAT GATGATGATC TTACTGATTC TGAAATGGAT
    GTGGTCAGGT TTGATGATGA CAACTCTCCT TCCTTTATCC AAATTCGCTC AGTTGCCAAG
    AAGCATCCTA AAACTTGGGT ACATTACATT GCTGCTGAAG AGGAGGACTG GGACTATGCT
    CCCTTAGTCC TCGCCCCCGA TGACAGAAGT TATAAAAGTC AATATTTGAA CAATGGCCCT
    CAGCGGATTG GTAGGAAGTA CAAAAAAGTC CGATTTATGG CATACACAGA TGAAACCTTT
    AAGACTCGTG AAGCTATTCA GCATGAATCA GGAATCTTGG GACCTTTACT TTATGGGGAA
    GTTGGAGACA CACTGTTGAT TATATTTAAG AATCAAGCAA GCAGACCATA TAACATCTAC
    CCTCACGGAA TCACTGATGT CCGTCCTTTG TATTCAAGGA GATTACCAAA AGGTGTAAAA
    CATTTGAAGG ATTTTCCAAT TCTGCCAGGA GAAATATTCA AATATAAATG GACAGTGACT
    GTAGAAGATG GGCCAACTAA ATCAGATCCT CGGTGCCTGA CCCGCTATTA CTCTAGTTTC
    GTTAATATGG AGAGAGATCT AGCTTCAGGA CTCATTGGCC CTCTCCTCAT CTGCTACAAA
    GAATCTGTAG ATCAAAGAGG AAACCAGATA ATGTCAGACA AGAGGAATGT CATCCTGTTT
    TCTGTATTTG ATGAGAACCG AAGCTGGTAC CTCACAGAGA ATATACAACG CTTTCTCCCC
    AATCCAGCTG GAGTGCAGCT TGAGGATCCA GAGTTCCAAG CCTCCAACAT CATGCACAGC
    ATCAATGGCT ATGTTTTTGA TAGTTTGCAG TTGTCAGTTT GTTTGCATGA GGTGGCATAC
    TGGTACATTC TAAGCATTGG AGCACAGACT GACTTCCTTT CTGTCTTCTT CTCTGGATAT
    ACCTTCAAAC ACAAAATGGT CTATGAAGAT ACACTCACCC TATTCCCATT CTCAGGAGAA
    ACTGTCTTCA TGTCGATGGA AAACCCAGGC CTATGGATTC TGGGGTGCCA CAACTCAGAC
    TTTCGGAACA GAGGCATGAC CGCCTTACTG AAGGTTTCTA GTTGTGACAA GAACACTGGT
    GATTATTACG AGGACAGTTA TGAAGATATT TCAGCATACT TGCTGAGTAA AAACAATGCC
    ATTGAACCAA GAAGCTTCTC CCAGAATTCA AGACACCCTA GCACTAGGCA AAAGCAATTT
    AATGCCACCA CAATTCCAGA AAATGACATA GAGAAGACTG ACCCTTGGTT TGCACACAGA
    ACACCTATGC CTAAAGTACA AAATGTCTCC TCTAGTGATT TGTTGATGCT CTTGCGACAG
    AGTCCTACTC CACATGGGCT ATCCTTATCT GATCTCCAAG AAGCCAAATA TGAGACTTTT
    TCTGATGATC CATCACCTGG AGCAATAGAC AGTAATAACA GCCTATCTGA AATGACACAC
    TTCAGGCCAC AGTTCCATCA CAGTGGGGAC ATGGTATTTA CCCCTGAGTC AGGCCTCCAA
    TTGAGATTAA ATGAGAAACT GGGGACAACT GCAGCAACAG AGTTGAAGAA ACTTGATTTC
    AAAGTTTCTA GTACATCAAA TAATCTGATT TCAACAATTC CATCAGACAA TTTGGCAGCA
    GGTACTGATA ATACAAGTTC CTTAGGACCC CCAAGTATGC CAGTTCATTA TGATAGTCAA
    TTAGATACCA CTCTATTTGG CAAAAAGTCA TCTCCCCTTA CTGAGTCTGG TAGACCTCTG
    AGCTTGAGTG AAGAAAATAA TGATTCAAAG TTGTTAGAAT CAGGTTTAAT GAATAGCCAA
    GAAAGTTCAT GGGGAAAAAA TGTATCGTCA ACAGAGAGTG GTAGGTTATT TAAAGGGAAA
    AGAGCTCATG GACCTGCTTT GTTGACTAAA GATAATGCCT TATTCAAAGT TAGCATCTCT
    TTGTTAAAGA CAAACAAAAC TTCCAATAAT TCAGCAACTA ATAGAAAGAC TCACATTGAT
    GGCCCATCAT TATTAATTCA GAGTAGTCCA TCAGTCTGGC AAAATATATT AGAAAGTGAC
    ACTGAGTTTA AAAAAGTGAC ACCTTTGATT CATGACAGAA TGCTTATGGA CAAAAATGCT
    ACAGCTTTGA GGCTAAATCA TATGTCAAAT AAAACTACTT CATCAAAAAA CATGGAAATG
    GTCCGACAGA AAAAAGAGGG CCCCATTCCA CCAGATGCAG AAAATCCAGA TATGTCATTC
    TTTAAGATGC TATTCTTGCC AGAATCAGCA AGTTGGATAC AAAGGACTCA TGGAAAGAAC
    TCTCTGAACT CTGGGCAAGG CCCCAGTCCA AAGCAATTAG TATCCTTAGG ACCAGAAAAA
    TCTGTGGAAG GTCAGAATTT CTTGTCTGAG AAAAACAAAG TGGTAGTAGG AAAGGGTGAA
    TTTACAAAGG ACGTAGGACT CAAAGAGATG GTTTTTCCAA GCAGCAGAAA CCTATTTCTT
    ACTAACTTGG ATAATTTACA TGAAAATAAT ACACACAATC AAGAAAAAAA AATTCAGGAA
    GAAATAGAAA GGAAGGAAAC ATTAATCCAA GAGAATGTAG TTTTGCCTCA GATACATACA
    GTGACTGGCA CTAAGAATTT CATGAAGAAC CTTTTCTTAC TGAGCACTAG GCAAAATGTA
    GAAGGTTCAT ATGAGGGGGC ATATGCTCCA GTACTTCAAG ATTTTAGGTC ATTAAATGAT
    TCAACAAATA GAACAAAGAA ACACACGGCT CATTTCTCAA AAAAAGGGGA AGAAGAAAAC
    TTGGCAGGCT TGGGAAATCA AACCAAGCAA ATTGTAGAGA AATATGCATG CACCACAAGG
    ATATCTCCTA ATACAAGCCA GCAGAATTTT GTCACGCAAC GTAGTAAGAG AGCTTTGAAA
    CAATTCAGAC TCCCACTAGA AGAAACAGAA CTTGAAAAAA GGATAATTGT GGATGACACC
    TCAACCCAGT GGTCCAAAAA CATGAAACAT TTGACCCCGA GCACCCTCAC ACAGATAGAC
    TACAATGAGA AGGAGAAAGG GGCCATTACT CAGTCTCCCT TATCAGATTG CCTTACGAGG
    AGTCATAGCA TCCCTCAAGC AAATAGATCT CCATTACCCA TTGCAGAGGT ATCATCATTT
    CCATCTATTA GACCTATATA TCTGACCAGG GTCCTATTCC AAGACAACTC TTCTCATCTT
    CCAGCAGCAT CTTATAGAAA GAAAGATTCT GGGGTCCAAG AAAGCAGTCA TTTCTTACAA
    GGAGCCAAAA AAAATAACCT TTCTTTAGCC ATTCTAACCT TGGAGATGAC TGGTGATCAA
    AGAGAGGTTG GCTCCCTGGG GACAAGTGCC ACAAATTCAG TCACTTACAA GAAAGTTGAG
    AACACTGTTC TCCCGAAACC AGGCTTGCCC AAAACATCTG GCAAAGTTGA ATTGCTTCCA
    AAAGTTCACA TTTATCAGAA GGACCTATTC CCTACGGAAA CTAGCAATGG GTCTCCTGGC
    CATCTGGATC TCGTGGAAGG GAGCCTTCTT CAGGAAACAG AGGGAGCGAT TAAGTGGAAT
    GAAGCAAACA GACCTGGAAA AATTCCCTTT CTGAGAGTAG CAACAGAAAG CTCTGCAAAG
    ACTCCCTCCA AGCTATTGGA TCCTCTTGCT TGGGATAACC ACTATGGTAC TCAGATACCA
    AAAGAAGAGT GGAAATCCCA AGAGAAGTCA CCAGAAAAAA CAGCTTTTAA GAAAAAGGAT
    ACCATTTTGT CCCTGAACGC TTGTGAAAGC AATCATGCAA TAGCAGCAAT AAATGAGGGA
    CAAAATAAGC CCGAAATAGA AGTCACCTGG GCAAAGCAAG GTAGGACTGA AAGGCTGTGC
    TCTCAAAACC CACCAGGCTT GAAACGCCAT CAACGGGAAA TAACTCGTAC TACTCTTCAG
    TCAGATCAAG AGGAAATTGA CTATGATGAT ACCATCTCAG TTGAAATGAA GAAGGAAGAT
    TTTGACATTT ATGATGAGGA TGAAAATCAG AGCCCCCGCA GCTTTCAAAA GAAAACACGA
    CACTATTTTA TTGCTGCAGT GGAGAGGCTC TGGGATTATG GGATGAGTAG CTCCCCACAT
    GTTCTAAGAA ACAGGGCTCA GAGTGGCAGT GTCCCTCAGT TCAAGAAAGT TGTTTTCCAG
    GAATTTACTG ATGGCTCCTT TACTCAGCCC TTATACCGTG GAGAACTAAA TGAACATTTG
    GGACTCCTGG GGCCATATAT AAGAGCAGAA GTTGAAGATA ATATCATGGT AACTTTCAAA
    AATCAGGCCT CTCGTCCCTA TTCCTTCTAT TCTAGCCTTA TTTCTTATGA GGAAGATCAG
    AGGCAAGGAG CAGAACCTAG GAAAAACTTT GTCAAGCCTA ATGAAACCAA AACTTACTTT
    TGGAAAGTGC AACATCATAT GGCGCCCACT AAAGATGAGT TTGACTGCAA AGCCTGGGCT
    TATTTCTCTG ATGTTGACCT GGAAAAAGAT GTGCACTCAG GCCTGATTGG ACCCCTTCTG
    GTCTGCCACA CTAACACACT GAACCCTGCT CATGGGAGAC AAGTGACAGT ACAGGAATTT
    GCTCTGTTTT TCACCATCTT TGATGAGACC AAAAGCTGGT ACTTCACTGA AAACATGGAA
    AGAAACTGCA GGGCTCCCTG CAATATCCAG ATGGAAGATC CCACTTTTAA AGAGAATTAT
    CGCTTCCATG CAATCAATGG CTACATAATG GATACACTAC CTGGCTTAGT AATGGCTCAG
    GATCAAAGGA TTCGATGGTA TCTGCTCAGC ATGGGCAGCA ATGAAAACAT CCATTCTATT
    CATTTCAGTG GACATGTGTT CACTGTACGA AAAAAAGAGG AGTATAAAAT GGCACTGTAC
    AATCTCTATC CAGGTGTTTT TGAGACAGTG GAAATGTTAC CATCCAAAGC TGGAATTTGG
    CGGGTGGAAT GCCTTATTGG CGAGCATCTA CATGCTGGGA TGAGCACACT TTTTCTGGTG
    TACAGCAATA AGTGTCAGAC TCCCCTGGGA ATGGCTTCTG GACACATTAG AGATTTTCAG
    ATTACAGCTT CAGGACAATA TGGACAGTGG GCCCCAAAGC TGGCCAGACT TCATTATTCC
    GGATCAATCA ATGCCTGGAG CACCAAGGAG CCCTTTTCTT GGATCAAGGT GGATCTGTTG
    GCACCAATGA TTATTCACGG CATCAAGACC CAGGGTGCCC GTCAGAAGTT CTCCAGCCTC
    TACATCTCTC AGTTTATCAT CATGTATAGT CTTGATGGGA AGAAGTGGCA GACTTATCGA
    GGAAATTCCA CTGGAACCTT AATGGTCTTC TTTGGCAATG TGGATTCATC TGGGATAAAA
    CACAATATTT TTAACCCTCC AATTATTGCT CGATACATCC GTTTGCACCC AACTCATTAT
    AGCATTCGCA GCACTCTTCG CATGGAGTTG ATGGGCTGTG ATTTAAATAG TTGCAGCATG
    CCATTGGGAA TGGAGAGTAA AGCAATATCA GATGCACAGA TTACTGCTTC ATCCTACTTT
    ACCAATATGT TTGCCACCTG GTCTCCTTCA AAAGCTCGAC TTCACCTCCA AGGGAGGAGT
    AATGCCTGGA GACCTCAGGT GAATAATCCA AAAGAGTGGC TGCAAGTGGA CTTCCAGAAG
    ACAATGAAAG TCACAGGAAT AACTACTCAG GGAGTAAAAT CTCTGCTTAC CAGCATGTAT
    GTGAAGGAGT TCCTCATCTC CAGCAGTCAA GATGGCCATC ACTGGACTCT CTTTTTTCAG
    AATGGCAAAG TAAAGGTTTT TCAGGGAAAT CAAGACTCCT TCACACCTGT GGTGAACTCT
    CTAGACCCAC CGTTACTGAC TCGCTACCTT CGAATTCACC CCCAGAGTTG GGTGCACCAG
    ATTGCCCTGA GGATGGAGGT TCTGGGCTGC GAGGCACAGG ACCTCTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc91300
    Clone ID Related Accession (Same CDS sequence) XM_003317788.3
    Accession Version XM_003317788.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product coagulation factor VIII isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006491.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_003317788.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGCTCGCCG CCCCAAGCAC TTCCCGACGC GCCGCCGCAG CTGGCGGGCG GGCCGGGGCG 
    GGGCGACGTG CCCTGCGTCC CCCTCGGCGG GCTGCCGCCG TGCCCGCGCC GGCTCCCCAG
    CCCGAGCCTG CCCCTTGCCC TGGTGATATG CAAAGAGCGG GATCGGAGGC GGGGCCTGGC
    CGGGCTGTGA GCGGCGTATG CAAATGGAGG GTCTCGGGGA TGCGGAGCCA AGACCCTGGG
    AAGGTCTTCT TTGGCAATGT GGATTCATCT GGGATAAAAC ACAATATTTT TAACCCTCCA
    ATTATTGCTC GATACATCCG TTTGCACCCA ACTCATTATA GCATTCGCAG CACTCTTCGC
    ATGGAGTTGA TGGGCTGTGA TTTAAATAGT TGCAGCATGC CATTGGGAAT GGAGAGTAAA
    GCAATATCAG ATGCACAGAT TACTGCTTCA TCCTACTTTA CCAATATGTT TGCCACCTGG
    TCTCCTTCAA AAGCTCGACT TCACCTCCAA GGGAGGAGTA ATGCCTGGAG ACCTCAGGTG
    AATAATCCAA AAGAGTGGCT GCAAGTGGAC TTCCAGAAGA CAATGAAAGT CACAGGAATA
    ACTACTCAGG GAGTAAAATC TCTGCTTACC AGCATGTATG TGAAGGAGTT CCTCATCTCC
    AGCAGTCAAG ATGGCCATCA CTGGACTCTC TTTTTTCAGA ATGGCAAAGT AAAGGTTTTT
    CAGGGAAATC AAGACTCCTT CACACCTGTG GTGAACTCTC TAGACCCACC GTTACTGACT
    CGCTACCTTC GAATTCACCC CCAGAGTTGG GTGCACCAGA TTGCCCTGAG GATGGAGGTT
    CTGGGCTGCG AGGCACAGGA CCTCTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003317836.2
    CDS24..893
    Translation

    Target ORF information:

    RefSeq Version XM_003317788.3
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes coagulation factor VIII (F8), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003317788.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGCTCGCCG CCCCAAGCAC TTCCCGACGC GCCGCCGCAG CTGGCGGGCG GGCCGGGGCG 
    GGGCGACGTG CCCTGCGTCC CCCTCGGCGG GCTGCCGCCG TGCCCGCGCC GGCTCCCCAG
    CCCGAGCCTG CCCCTTGCCC TGGTGATATG CAAAGAGCGG GATCGGAGGC GGGGCCTGGC
    CGGGCTGTGA GCGGCGTATG CAAATGGAGG GTCTCGGGGA TGCGGAGCCA AGACCCTGGG
    AAGGTCTTCT TTGGCAATGT GGATTCATCT GGGATAAAAC ACAATATTTT TAACCCTCCA
    ATTATTGCTC GATACATCCG TTTGCACCCA ACTCATTATA GCATTCGCAG CACTCTTCGC
    ATGGAGTTGA TGGGCTGTGA TTTAAATAGT TGCAGCATGC CATTGGGAAT GGAGAGTAAA
    GCAATATCAG ATGCACAGAT TACTGCTTCA TCCTACTTTA CCAATATGTT TGCCACCTGG
    TCTCCTTCAA AAGCTCGACT TCACCTCCAA GGGAGGAGTA ATGCCTGGAG ACCTCAGGTG
    AATAATCCAA AAGAGTGGCT GCAAGTGGAC TTCCAGAAGA CAATGAAAGT CACAGGAATA
    ACTACTCAGG GAGTAAAATC TCTGCTTACC AGCATGTATG TGAAGGAGTT CCTCATCTCC
    AGCAGTCAAG ATGGCCATCA CTGGACTCTC TTTTTTCAGA ATGGCAAAGT AAAGGTTTTT
    CAGGGAAATC AAGACTCCTT CACACCTGTG GTGAACTCTC TAGACCCACC GTTACTGACT
    CGCTACCTTC GAATTCACCC CCAGAGTTGG GTGCACCAGA TTGCCCTGAG GATGGAGGTT
    CTGGGCTGCG AGGCACAGGA CCTCTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.