GLI3 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene
Clones
gRNAs

Summary

Gene Symbol	GLI3
Entrez Gene ID	463369
Full Name	GLI family zinc finger 3
Synonyms	GLI3-190,GLI3FL
General protein information	Preferred Names GLI family zinc finger 3 Names transcriptional activator GLI3 GLI-Kruppel family member 3 GLI-Kruppel family member GLI3 (Greig cephalopolysyndactyly syndrome) GLI3 form of 190 kDa GLI3 full length protein zinc finger protein GLI3
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009452941.3	XP_009451216.1	transcriptional activator GLI3 isoform X1
XM_009452941.2	XP_009451216.1	transcriptional activator GLI3 isoform X1
XM_016956811.2	XP_016812300.1	transcriptional activator GLI3 isoform X2
NM_001034190.1	NP_001029362.1	transcriptional activator GLI3
XM_016956811.1	XP_016812300.1	transcriptional activator GLI3 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	GLI3	NP_000159.3
Rattus norvegicus (Norway rat)	Gli3	NP_536330.1
Danio rerio (zebrafish)	gli3	NP_991291.1
Pan troglodytes (chimpanzee)	GLI3	NP_001029362.1
Canis lupus familiaris (dog)	GLI3	XP_005630984.1
Bos taurus (cattle)	LOC785371	XP_005205717.1
Gallus gallus (chicken)	GLI3	NP_001258832.1
	GLI3	XP_001098108.1
Mus musculus (house mouse)	Gli3	NP_032156.2
Xenopus tropicalis (tropical clawed frog)	gli3	XP_004915408.1

Gene Ontology
Bibliography

Related articles in PubMed

Accelerated evolution of nervous system genes in the origin of Homo sapiens.
Dorus S, Vallender EJ, Evans PD, Anderson JR, Gilbert SL, Mahowald M, Wyckoff GJ, Malcom CM, Lahn BT
Cell119(7)1027-40(2004 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GLI3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GLI3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc107656	XM_009452941.3 Latest version!	Pan troglodytes GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPc107657	XM_016956811.2 Latest version!	Pan troglodytes GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPc56900	XM_016956811.1 Latest version!	Pan troglodytes GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc00006	NM_001034190.1 Latest version!	Pan troglodytes GLI family zinc finger 3 (GLI3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OPc40512	XM_009452941.2 Latest version!	Pan troglodytes GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GLI3 ( XM_009452941.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009451216.1 Pan troglodytes GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPc107656
Clone ID Related Accession (Same CDS sequence)	XM_009452941.3
Accession Version	XM_009452941.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	transcriptional activator GLI3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036886.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009452941.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCC AGTCCCACAG CTCCACGACC ACTGAAAAGA AAAAAGTTGA GAATTCCATA 
GTGAAGTGCT CCACTCGAAC AGATGTGAGC GAGAAAGCCG TTGCCTCCAG CACCACTTCT 
AATGAGGATG AAAGTCCTGG ACAGACTTAT CACAGAGAGA GAAGAAACGC AATCACTATG 
CAGCCACAGA ATGTCCAGGG GCTCAGCAAA GTCAGTGAGG AACCTTCAAC ATCGAGTGAC 
GAGAGGGCCT CATTGATCAA GAAAGAGATC CATGGGTCCC TGCCACACGT GGCAGAGCCC 
TCTGTGCCGT ACCGCGGGAC GGTGTTTGCC ATGGACCCCA GGAATGGTTA CATGGAGCCC 
CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA 
CATCATGAGG GCCGTTACCA TTACGATCCG TCTCCGATTC CTCCATTGCA TATGACTTCC 
GCCTTATCTA GTAGCCCTAC GTATCCGGAC CTGCCCTTCA TTAGGATCTC CCCACACCGG 
AACCCCGCTG CTGCTTCCGA GTCTCCCTTC AGCCCTCCAC ATCCCTACAT TAATCCCTAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCGCTCT CCATGATCTC AGCAACCCGT 
GGGCTGAGCC CTACAGATGC GCCCCATGCA GGAGTCAGCC CAGCAGAATA CTATCATCAG 
ATGGCCCTGC TAACTGGCCA GCGCAGCCCC TATGCAGACA TTATTCCCTC GGCTGCCACC 
GCCGGCACGG GGGCCATCCA CATGGAATAT CTTCATGCTA TGGATAGCAC CAGATTCCCC 
AGCCCCAGGC TGTCAGCCAG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTGTCC 
GATCATAGCT TTGACCTTCA GACCATGATA AGGACGTCTC CCAACTCCTT GGTCACGATT 
CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGCTCCT ATGGTCACTT ATCTGCAAGT 
GCAATCAGCC CTGCCTTGAG CTTCACCTAC TCTTCCGCGC CCGTCTCTCT CCACATGCAT 
CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGTTCAGCCT TTGGACACAG CCCTCCACTC 
ATCCACCCTG CCCCAACTTT TCCAACACAG AGGCCTATTC CAGGGATCCC TACGGTTCTG 
AACCCCGTCC AGGTCAGCTC CGGCCCTTCT GAGTCCTCAC AGAACAAGCC CACGAGTGAG 
TCTGCAGTGA GTAGCACTGG TGACCCGATG CACAACAAGA GGTCCAAGAT CAAACCCGAT 
GAAGACCTCC CCAGCCCAGG GGCTCGGGGG CAGCAGGAAC AGCCCGAAGG AACAACCCTT 
GTCAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CTGAAGTCAT CTATGAGACA 
AACTGTCACT GGGAAGGCTG CGCGAGGGAG TTCGACACCC AAGAGCAGCT TGTGCACCAT 
ATAAATAATG ACCATATTCA TGGAGAGAAG AAGGAGTTCG TGTGCAGGTG GCTGGACTGC 
TCAAGAGAGC AGAAACCCTT CAAAGCCCAG TATATGTTGG TAGTGCATAT GAGAAGACAC 
ACGGGCGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTA 
GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC 
GAAGGTTGCA ACAAGGCTTT CTCAAACGCC TCTGATCGCG CCAAACACCA AAACAGAACG 
CATTCCAATG AGAAACCATA TGTGTGCAAA ATCCCAGGCT GCACTAAGCG TTACACAGAC 
CCAAGCTCCC TCCGGAAACA TGTGAAGACA GTGCATGGCC CAGAGGCTCA TGTCACCAAG 
AAGCAGCGAG GGGACATCCA TCCTCGGCCA CCACCCCCGA GAGATTCCGG CAGCCATTCA 
CAGTCCAGGT CGCCCGGCCG ACCGACTCAG GGAGCCCTTG GTGAGCAGCA GGACCTCAGC 
AACACTACCT CAAAGCGGGA AGAATGCCTC CAGGTGAAAA CCGTCAAGGC AGAGAAGCCA 
ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC 
AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTAG TATAGGAGAC 
CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCAACCA TTTCCACTGC AACCACAGCC 
CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CGTAAAACTA 
GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCGCGACTGA ACCCCATTCT ACCCCCTAAA 
GCCCCTGCGG TCTCTCCTCT CATAGGAAAT GGCACACAGT CCAACAACAC CTGCAGCTTG 
GGTGGGCCCA TGACGCTTCT CCCGGGCAGA AGCGACCTCT CTGGGGTGGA CGTCACTATG 
CTGAACATGC TCAACAGAAG GGACAGCAGC GCCAGCACCA TCAGCTCGGC CTACCTGAGC 
AGCCGCCGCT CCTCAGGGAT CTCGCCCTGC TTCTCCAGCC GCCGCTCCAG CGAGGCGTCA 
CAGGCCGAGG GCCGGCCGCA GAACGTGAGC GTGGCCGACT CCTACGACCC CATCTCCACC 
GACGCCTCGC GCCGCTCCAG CGAAGCCAGC CAGAGCGACG GCCTGCCCAG CCTGCTCAGC 
CTCACGCCCG CCCAGCAGTA CCGCCTCAAG GCCAAGTACG CGGCTGCCAC AGGAGGGCCG 
CCGCCCACGC CCCTGCCCAA CATGGAGAGG ATGAGCCTGA AGACGCGCCT GGCGCTGCTC 
GGGGATGCCC TCGAGCCTGG CGTGGCCCTG CCTCCAGTTC ATGCCCCGAG GAGGTGCAGC 
GACGGGGGAG CCCACGGCTA CGGGCGGCGC CACCTGCAGC CGCACGATGC GCCGGGCCAC 
GGCGTGAGGA GGGCCAGCGA CCCCGTGCGG ACAGGCTCCG AGGGCCTGGC CCTGCCTCGT 
GTGCCGCGCT TCAGCAGCCT CAGCAGCTGC AACCCCCCGG CGATGGCCAC GTCCGCGGAG 
AAGCGCAGTC TCGTGCTTCA GAATTACACG CGGCCCGAGG GCGGCCAGTC CCGAAACTTC 
CACTCGTCCC CCTGTCCTCC CAGCATCACC GAGAACGTCA CCCTGGAGTC CCTGACCATG 
GACGCTGATG CCAACCTGAA CGATGAGGAT TTCCTGCCGG ACGACGTGGT GCAGTATTTA 
AATTCCCAGA ACCAAGCAGG GTACGAGCAG CACTTCCCCA GCGCCCTCCC GGACGACAGC 
AAAGTGCCCC ACGGGCCCGG TGACTTTGAC GCGCCCGGGC TGCCAGACAG CCACGCTGGC 
CAGCAGTTCC ATGCCCTCGA GCAGCCCTGC CCCGAGGGCA GCAAAACCGA CCTGCCCATT 
CAGTGGAACG AAGTCAGCTC CGGAAGCGCC GACCTGTCCT CCTCCAAGCT CAAGTGTGGG 
CCGCGGCCCG CTGTGCCGCA GACTCGCGCC TTTGGGTTCT GCAACGGCAT GGTCGTCCAC 
CCGCAGAACC CCTTGAGGAG CGGGCCTGCT GGGGGCTATC AGACCCTCGG GGAGAACAGC 
AACCCCTACG GTGGCCCAGA GCACTTGATG CTCCACAACA GCCCCGGAAG TGGCACCAGT 
GGAAACGCCT TCCATGAACA GCCCTGTAAG GCCCCGCAGT ATGGGAACTG TCTCAACAGG 
CAGCCAGTGG CCCCTGGTGC ACTCGACGGT GCCTGTGGTG CCGGGATTCA AGCCTCAAAG 
CTGAAGAGCA CCCCCATGCA AGGGAGCGGG GGCCAGCTGA ATTTCGGCCT GCCGGTAGCG 
CCAAATGAGT CAGCTGGCAG CATGGTGAAT GGCATGCAGA ACCAGGACCC AGTGGGACAG 
GGGTACCTGG CTCACCAGCT CCTCGGCGAC AGCATGCAGC ACCCGGGGGC AGGCCGCCCC 
GGTCAGCAGA TGCTTGGGCA GATTAGTGCT ACCTCACACA TCAACATCTA TCAAGGGCCA 
GAGAGCTGCC TGCCAGGGGC TCACGGCATG GGCAGCCAGC CGTCAAGCTT GGCAGTTGTC 
AGGGGCTACC AGCCATGTGC CAGCTTTGGG GGCAGCAGGC GCCAGGCTAT GCCGAGGGAC 
AGCCTTGCTC TGCAGTCAGG ACAGCTCAGT GACACAAGTC AGACCTGCAG GGTGAATGGC 
ATCAAGATGG AGATGAAAGG GCAGCCCCAT CCGCTGTGCT CTAATCTGCA GAATTACTCT 
GGTCAGTTCT ATGACCAAAC CGTGGGCTTC AGTCAGCAAG ACACGAAAGC TGGTTCATTC 
TCTATTTCAG ACGCCAGCTG CCTGCTACAG GGGACCAGCG CCAAAAACTC TGAGTTACTT 
TCCCCAGGTG CTAATCAGGT GACAAGCACA GTGGACAGCC TCGACAGCCA TGACCTGGAA 
GGGGTACAGA TTGACTTCGA TGCCATCATA GACGATGGGG ACCACTCCAG CCTGATGTCG 
GGGGCCCTGA GCCCAAGTAT CATTCAGAAC CTTTCCTATA GCTCCTCCCG CCTCACCACG 
CCTCGGGCGT CCCTCCCATT CCCAGCGCTG TCCATGAGCA CCACCAACAT GGCTATCGGG 
GACATGAGTT CTTTGCTGAC CTCCCTAGCG GAAGAAAGCA AATTCCTTGC AGTTATGCAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009451216.1
CDS	201..4943
Translation	MEAQSHSSTTTEKKKVENSIVKCSTRTDVSEKAVASSTTSNEDESPGQTYHRERRNAITMQPQNVQGLSKVSEEPSTSSDERASLIKKEIHGSLPHVAEPSVPYRGTVFAMDPRNGYMEPHYHPPHLFPAFHPPVPIDARHHEGRYHYDPSPIPPLHMTSALSSSPTYPDLPFIRISPHRNPAAASESPFSPPHPYINPYMDYIRSLHSSPSLSMISATRGLSPTDAPHAGVSPAEYYHQMALLTGQRSPYADIIPSAATAGTGAIHMEYLHAMDSTRFPSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYSSAPVSLHMHQQILSRQQSLGSAFGHSPPLIHPAPTFPTQRPIPGIPTVLNPVQVSSGPSESSQNKPTSESAVSSTGDPMHNKRSKIKPDEDLPSPGARGQQEQPEGTTLVKEEGDKDESKQEPEVIYETNCHWEGCAREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPRDSGSHSQSRSPGRPTQGALGEQQDLSNTTSKREECLQVKTVKAEKPMTSQPSPGGQSSCSSQQSPISNYSNSGLELPLTDGGSIGDLSAIDETPIMDSTISTATTALALQARRNPAGTKWMEHVKLERLKQVNGMFPRLNPILPPKAPAVSPLIGNGTQSNNTCSLGGPMTLLPGRSDLSGVDVTMLNMLNRRDSSASTISSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSEASQSDGLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRLALLGDALEPGVALPPVHAPRRCSDGGAHGYGRRHLQPHDAPGHGVRRASDPVRTGSEGLALPRVPRFSSLSSCNPPAMATSAEKRSLVLQNYTRPEGGQSRNFHSSPCPPSITENVTLESLTMDADANLNDEDFLPDDVVQYLNSQNQAGYEQHFPSALPDDSKVPHGPGDFDAPGLPDSHAGQQFHALEQPCPEGSKTDLPIQWNEVSSGSADLSSSKLKCGPRPAVPQTRAFGFCNGMVVHPQNPLRSGPAGGYQTLGENSNPYGGPEHLMLHNSPGSGTSGNAFHEQPCKAPQYGNCLNRQPVAPGALDGACGAGIQASKLKSTPMQGSGGQLNFGLPVAPNESAGSMVNGMQNQDPVGQGYLAHQLLGDSMQHPGAGRPGQQMLGQISATSHINIYQGPESCLPGAHGMGSQPSSLAVVRGYQPCASFGGSRRQAMPRDSLALQSGQLSDTSQTCRVNGIKMEMKGQPHPLCSNLQNYSGQFYDQTVGFSQQDTKAGSFSISDASCLLQGTSAKNSELLSPGANQVTSTVDSLDSHDLEGVQIDFDAIIDDGDHSSLMSGALSPSIIQNLSYSSSRLTTPRASLPFPALSMSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	XM_009452941.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009452941.3

ORF Insert Sequence:

ATGGAGGCCC AGTCCCACAG CTCCACGACC ACTGAAAAGA AAAAAGTTGA GAATTCCATA 
GTGAAGTGCT CCACTCGAAC AGATGTGAGC GAGAAAGCCG TTGCCTCCAG CACCACTTCT 
AATGAGGATG AAAGTCCTGG ACAGACTTAT CACAGAGAGA GAAGAAACGC AATCACTATG 
CAGCCACAGA ATGTCCAGGG GCTCAGCAAA GTCAGTGAGG AACCTTCAAC ATCGAGTGAC 
GAGAGGGCCT CATTGATCAA GAAAGAGATC CATGGGTCCC TGCCACACGT GGCAGAGCCC 
TCTGTGCCGT ACCGCGGGAC GGTGTTTGCC ATGGACCCCA GGAATGGTTA CATGGAGCCC 
CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA 
CATCATGAGG GCCGTTACCA TTACGATCCG TCTCCGATTC CTCCATTGCA TATGACTTCC 
GCCTTATCTA GTAGCCCTAC GTATCCGGAC CTGCCCTTCA TTAGGATCTC CCCACACCGG 
AACCCCGCTG CTGCTTCCGA GTCTCCCTTC AGCCCTCCAC ATCCCTACAT TAATCCCTAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCGCTCT CCATGATCTC AGCAACCCGT 
GGGCTGAGCC CTACAGATGC GCCCCATGCA GGAGTCAGCC CAGCAGAATA CTATCATCAG 
ATGGCCCTGC TAACTGGCCA GCGCAGCCCC TATGCAGACA TTATTCCCTC GGCTGCCACC 
GCCGGCACGG GGGCCATCCA CATGGAATAT CTTCATGCTA TGGATAGCAC CAGATTCCCC 
AGCCCCAGGC TGTCAGCCAG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTGTCC 
GATCATAGCT TTGACCTTCA GACCATGATA AGGACGTCTC CCAACTCCTT GGTCACGATT 
CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGCTCCT ATGGTCACTT ATCTGCAAGT 
GCAATCAGCC CTGCCTTGAG CTTCACCTAC TCTTCCGCGC CCGTCTCTCT CCACATGCAT 
CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGTTCAGCCT TTGGACACAG CCCTCCACTC 
ATCCACCCTG CCCCAACTTT TCCAACACAG AGGCCTATTC CAGGGATCCC TACGGTTCTG 
AACCCCGTCC AGGTCAGCTC CGGCCCTTCT GAGTCCTCAC AGAACAAGCC CACGAGTGAG 
TCTGCAGTGA GTAGCACTGG TGACCCGATG CACAACAAGA GGTCCAAGAT CAAACCCGAT 
GAAGACCTCC CCAGCCCAGG GGCTCGGGGG CAGCAGGAAC AGCCCGAAGG AACAACCCTT 
GTCAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CTGAAGTCAT CTATGAGACA 
AACTGTCACT GGGAAGGCTG CGCGAGGGAG TTCGACACCC AAGAGCAGCT TGTGCACCAT 
ATAAATAATG ACCATATTCA TGGAGAGAAG AAGGAGTTCG TGTGCAGGTG GCTGGACTGC 
TCAAGAGAGC AGAAACCCTT CAAAGCCCAG TATATGTTGG TAGTGCATAT GAGAAGACAC 
ACGGGCGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTA 
GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC 
GAAGGTTGCA ACAAGGCTTT CTCAAACGCC TCTGATCGCG CCAAACACCA AAACAGAACG 
CATTCCAATG AGAAACCATA TGTGTGCAAA ATCCCAGGCT GCACTAAGCG TTACACAGAC 
CCAAGCTCCC TCCGGAAACA TGTGAAGACA GTGCATGGCC CAGAGGCTCA TGTCACCAAG 
AAGCAGCGAG GGGACATCCA TCCTCGGCCA CCACCCCCGA GAGATTCCGG CAGCCATTCA 
CAGTCCAGGT CGCCCGGCCG ACCGACTCAG GGAGCCCTTG GTGAGCAGCA GGACCTCAGC 
AACACTACCT CAAAGCGGGA AGAATGCCTC CAGGTGAAAA CCGTCAAGGC AGAGAAGCCA 
ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC 
AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTAG TATAGGAGAC 
CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCAACCA TTTCCACTGC AACCACAGCC 
CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CGTAAAACTA 
GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCGCGACTGA ACCCCATTCT ACCCCCTAAA 
GCCCCTGCGG TCTCTCCTCT CATAGGAAAT GGCACACAGT CCAACAACAC CTGCAGCTTG 
GGTGGGCCCA TGACGCTTCT CCCGGGCAGA AGCGACCTCT CTGGGGTGGA CGTCACTATG 
CTGAACATGC TCAACAGAAG GGACAGCAGC GCCAGCACCA TCAGCTCGGC CTACCTGAGC 
AGCCGCCGCT CCTCAGGGAT CTCGCCCTGC TTCTCCAGCC GCCGCTCCAG CGAGGCGTCA 
CAGGCCGAGG GCCGGCCGCA GAACGTGAGC GTGGCCGACT CCTACGACCC CATCTCCACC 
GACGCCTCGC GCCGCTCCAG CGAAGCCAGC CAGAGCGACG GCCTGCCCAG CCTGCTCAGC 
CTCACGCCCG CCCAGCAGTA CCGCCTCAAG GCCAAGTACG CGGCTGCCAC AGGAGGGCCG 
CCGCCCACGC CCCTGCCCAA CATGGAGAGG ATGAGCCTGA AGACGCGCCT GGCGCTGCTC 
GGGGATGCCC TCGAGCCTGG CGTGGCCCTG CCTCCAGTTC ATGCCCCGAG GAGGTGCAGC 
GACGGGGGAG CCCACGGCTA CGGGCGGCGC CACCTGCAGC CGCACGATGC GCCGGGCCAC 
GGCGTGAGGA GGGCCAGCGA CCCCGTGCGG ACAGGCTCCG AGGGCCTGGC CCTGCCTCGT 
GTGCCGCGCT TCAGCAGCCT CAGCAGCTGC AACCCCCCGG CGATGGCCAC GTCCGCGGAG 
AAGCGCAGTC TCGTGCTTCA GAATTACACG CGGCCCGAGG GCGGCCAGTC CCGAAACTTC 
CACTCGTCCC CCTGTCCTCC CAGCATCACC GAGAACGTCA CCCTGGAGTC CCTGACCATG 
GACGCTGATG CCAACCTGAA CGATGAGGAT TTCCTGCCGG ACGACGTGGT GCAGTATTTA 
AATTCCCAGA ACCAAGCAGG GTACGAGCAG CACTTCCCCA GCGCCCTCCC GGACGACAGC 
AAAGTGCCCC ACGGGCCCGG TGACTTTGAC GCGCCCGGGC TGCCAGACAG CCACGCTGGC 
CAGCAGTTCC ATGCCCTCGA GCAGCCCTGC CCCGAGGGCA GCAAAACCGA CCTGCCCATT 
CAGTGGAACG AAGTCAGCTC CGGAAGCGCC GACCTGTCCT CCTCCAAGCT CAAGTGTGGG 
CCGCGGCCCG CTGTGCCGCA GACTCGCGCC TTTGGGTTCT GCAACGGCAT GGTCGTCCAC 
CCGCAGAACC CCTTGAGGAG CGGGCCTGCT GGGGGCTATC AGACCCTCGG GGAGAACAGC 
AACCCCTACG GTGGCCCAGA GCACTTGATG CTCCACAACA GCCCCGGAAG TGGCACCAGT 
GGAAACGCCT TCCATGAACA GCCCTGTAAG GCCCCGCAGT ATGGGAACTG TCTCAACAGG 
CAGCCAGTGG CCCCTGGTGC ACTCGACGGT GCCTGTGGTG CCGGGATTCA AGCCTCAAAG 
CTGAAGAGCA CCCCCATGCA AGGGAGCGGG GGCCAGCTGA ATTTCGGCCT GCCGGTAGCG 
CCAAATGAGT CAGCTGGCAG CATGGTGAAT GGCATGCAGA ACCAGGACCC AGTGGGACAG 
GGGTACCTGG CTCACCAGCT CCTCGGCGAC AGCATGCAGC ACCCGGGGGC AGGCCGCCCC 
GGTCAGCAGA TGCTTGGGCA GATTAGTGCT ACCTCACACA TCAACATCTA TCAAGGGCCA 
GAGAGCTGCC TGCCAGGGGC TCACGGCATG GGCAGCCAGC CGTCAAGCTT GGCAGTTGTC 
AGGGGCTACC AGCCATGTGC CAGCTTTGGG GGCAGCAGGC GCCAGGCTAT GCCGAGGGAC 
AGCCTTGCTC TGCAGTCAGG ACAGCTCAGT GACACAAGTC AGACCTGCAG GGTGAATGGC 
ATCAAGATGG AGATGAAAGG GCAGCCCCAT CCGCTGTGCT CTAATCTGCA GAATTACTCT 
GGTCAGTTCT ATGACCAAAC CGTGGGCTTC AGTCAGCAAG ACACGAAAGC TGGTTCATTC 
TCTATTTCAG ACGCCAGCTG CCTGCTACAG GGGACCAGCG CCAAAAACTC TGAGTTACTT 
TCCCCAGGTG CTAATCAGGT GACAAGCACA GTGGACAGCC TCGACAGCCA TGACCTGGAA 
GGGGTACAGA TTGACTTCGA TGCCATCATA GACGATGGGG ACCACTCCAG CCTGATGTCG 
GGGGCCCTGA GCCCAAGTAT CATTCAGAAC CTTTCCTATA GCTCCTCCCG CCTCACCACG 
CCTCGGGCGT CCCTCCCATT CCCAGCGCTG TCCATGAGCA CCACCAACAT GGCTATCGGG 
GACATGAGTT CTTTGCTGAC CTCCCTAGCG GAAGAAAGCA AATTCCTTGC AGTTATGCAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI3 ( XM_016956811.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812300.1 Pan troglodytes GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPc107657
Clone ID Related Accession (Same CDS sequence)	XM_016956811.2
Accession Version	XM_016956811.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	transcriptional activator GLI3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036886.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016956811.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCAC AGAATGTCCA GGGGCTCAGC AAAGTCAGTG AGGAACCTTC AACATCGAGT 
GACGAGAGGG CCTCATTGAT CAAGAAAGAG ATCCATGGGT CCCTGCCACA CGTGGCAGAG 
CCCTCTGTGC CGTACCGCGG GACGGTGTTT GCCATGGACC CCAGGAATGG TTACATGGAG 
CCCCACTACC ACCCTCCTCA TCTTTTCCCT GCCTTCCATC CTCCTGTACC AATTGATGCC 
AGACATCATG AGGGCCGTTA CCATTACGAT CCGTCTCCGA TTCCTCCATT GCATATGACT 
TCCGCCTTAT CTAGTAGCCC TACGTATCCG GACCTGCCCT TCATTAGGAT CTCCCCACAC 
CGGAACCCCG CTGCTGCTTC CGAGTCTCCC TTCAGCCCTC CACATCCCTA CATTAATCCC 
TACATGGACT ATATCCGCTC CTTGCACAGC AGCCCATCGC TCTCCATGAT CTCAGCAACC 
CGTGGGCTGA GCCCTACAGA TGCGCCCCAT GCAGGAGTCA GCCCAGCAGA ATACTATCAT 
CAGATGGCCC TGCTAACTGG CCAGCGCAGC CCCTATGCAG ACATTATTCC CTCGGCTGCC 
ACCGCCGGCA CGGGGGCCAT CCACATGGAA TATCTTCATG CTATGGATAG CACCAGATTC 
CCCAGCCCCA GGCTGTCAGC CAGGCCGAGC CGAAAACGTA CACTGTCCAT ATCACCACTG 
TCCGATCATA GCTTTGACCT TCAGACCATG ATAAGGACGT CTCCCAACTC CTTGGTCACG 
ATTCTCAATA ATTCCCGTAG CAGCTCTTCA GCAAGTGGCT CCTATGGTCA CTTATCTGCA 
AGTGCAATCA GCCCTGCCTT GAGCTTCACC TACTCTTCCG CGCCCGTCTC TCTCCACATG 
CATCAGCAGA TCCTAAGCCG ACAACAGAGC TTAGGTTCAG CCTTTGGACA CAGCCCTCCA 
CTCATCCACC CTGCCCCAAC TTTTCCAACA CAGAGGCCTA TTCCAGGGAT CCCTACGGTT 
CTGAACCCCG TCCAGGTCAG CTCCGGCCCT TCTGAGTCCT CACAGAACAA GCCCACGAGT 
GAGTCTGCAG TGAGTAGCAC TGGTGACCCG ATGCACAACA AGAGGTCCAA GATCAAACCC 
GATGAAGACC TCCCCAGCCC AGGGGCTCGG GGGCAGCAGG AACAGCCCGA AGGAACAACC 
CTTGTCAAGG AGGAAGGGGA CAAAGATGAA AGCAAACAGG AGCCTGAAGT CATCTATGAG 
ACAAACTGTC ACTGGGAAGG CTGCGCGAGG GAGTTCGACA CCCAAGAGCA GCTTGTGCAC 
CATATAAATA ATGACCATAT TCATGGAGAG AAGAAGGAGT TCGTGTGCAG GTGGCTGGAC 
TGCTCAAGAG AGCAGAAACC CTTCAAAGCC CAGTATATGT TGGTAGTGCA TATGAGAAGA 
CACACGGGCG AGAAGCCTCA CAAATGCACT TTTGAAGGTT GCACAAAGGC CTACTCGAGA 
CTAGAAAACT TGAAAACACA CTTGAGATCT CACACTGGAG AGAAACCATA CGTCTGTGAG 
CACGAAGGTT GCAACAAGGC TTTCTCAAAC GCCTCTGATC GCGCCAAACA CCAAAACAGA 
ACGCATTCCA ATGAGAAACC ATATGTGTGC AAAATCCCAG GCTGCACTAA GCGTTACACA 
GACCCAAGCT CCCTCCGGAA ACATGTGAAG ACAGTGCATG GCCCAGAGGC TCATGTCACC 
AAGAAGCAGC GAGGGGACAT CCATCCTCGG CCACCACCCC CGAGAGATTC CGGCAGCCAT 
TCACAGTCCA GGTCGCCCGG CCGACCGACT CAGGGAGCCC TTGGTGAGCA GCAGGACCTC 
AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAGGTGA AAACCGTCAA GGCAGAGAAG 
CCAATGACAT CTCAGCCAAG CCCTGGTGGT CAGTCTTCAT GCAGCAGCCA ACAGTCCCCC 
ATCAGCAACT ATTCCAACAG TGGGCTCGAG CTTCCTCTGA CCGATGGAGG TAGTATAGGA 
GACCTCAGTG CCATCGATGA AACCCCAATC ATGGACTCAA CCATTTCCAC TGCAACCACA 
GCCCTTGCTT TGCAAGCCAG GAGAAACCCG GCAGGGACCA AATGGATGGA GCACGTAAAA 
CTAGAAAGGC TAAAACAAGT GAATGGAATG TTTCCGCGAC TGAACCCCAT TCTACCCCCT 
AAAGCCCCTG CGGTCTCTCC TCTCATAGGA AATGGCACAC AGTCCAACAA CACCTGCAGC 
TTGGGTGGGC CCATGACGCT TCTCCCGGGC AGAAGCGACC TCTCTGGGGT GGACGTCACT 
ATGCTGAACA TGCTCAACAG AAGGGACAGC AGCGCCAGCA CCATCAGCTC GGCCTACCTG 
AGCAGCCGCC GCTCCTCAGG GATCTCGCCC TGCTTCTCCA GCCGCCGCTC CAGCGAGGCG 
TCACAGGCCG AGGGCCGGCC GCAGAACGTG AGCGTGGCCG ACTCCTACGA CCCCATCTCC 
ACCGACGCCT CGCGCCGCTC CAGCGAAGCC AGCCAGAGCG ACGGCCTGCC CAGCCTGCTC 
AGCCTCACGC CCGCCCAGCA GTACCGCCTC AAGGCCAAGT ACGCGGCTGC CACAGGAGGG 
CCGCCGCCCA CGCCCCTGCC CAACATGGAG AGGATGAGCC TGAAGACGCG CCTGGCGCTG 
CTCGGGGATG CCCTCGAGCC TGGCGTGGCC CTGCCTCCAG TTCATGCCCC GAGGAGGTGC 
AGCGACGGGG GAGCCCACGG CTACGGGCGG CGCCACCTGC AGCCGCACGA TGCGCCGGGC 
CACGGCGTGA GGAGGGCCAG CGACCCCGTG CGGACAGGCT CCGAGGGCCT GGCCCTGCCT 
CGTGTGCCGC GCTTCAGCAG CCTCAGCAGC TGCAACCCCC CGGCGATGGC CACGTCCGCG 
GAGAAGCGCA GTCTCGTGCT TCAGAATTAC ACGCGGCCCG AGGGCGGCCA GTCCCGAAAC 
TTCCACTCGT CCCCCTGTCC TCCCAGCATC ACCGAGAACG TCACCCTGGA GTCCCTGACC 
ATGGACGCTG ATGCCAACCT GAACGATGAG GATTTCCTGC CGGACGACGT GGTGCAGTAT 
TTAAATTCCC AGAACCAAGC AGGGTACGAG CAGCACTTCC CCAGCGCCCT CCCGGACGAC 
AGCAAAGTGC CCCACGGGCC CGGTGACTTT GACGCGCCCG GGCTGCCAGA CAGCCACGCT 
GGCCAGCAGT TCCATGCCCT CGAGCAGCCC TGCCCCGAGG GCAGCAAAAC CGACCTGCCC 
ATTCAGTGGA ACGAAGTCAG CTCCGGAAGC GCCGACCTGT CCTCCTCCAA GCTCAAGTGT 
GGGCCGCGGC CCGCTGTGCC GCAGACTCGC GCCTTTGGGT TCTGCAACGG CATGGTCGTC 
CACCCGCAGA ACCCCTTGAG GAGCGGGCCT GCTGGGGGCT ATCAGACCCT CGGGGAGAAC 
AGCAACCCCT ACGGTGGCCC AGAGCACTTG ATGCTCCACA ACAGCCCCGG AAGTGGCACC 
AGTGGAAACG CCTTCCATGA ACAGCCCTGT AAGGCCCCGC AGTATGGGAA CTGTCTCAAC 
AGGCAGCCAG TGGCCCCTGG TGCACTCGAC GGTGCCTGTG GTGCCGGGAT TCAAGCCTCA 
AAGCTGAAGA GCACCCCCAT GCAAGGGAGC GGGGGCCAGC TGAATTTCGG CCTGCCGGTA 
GCGCCAAATG AGTCAGCTGG CAGCATGGTG AATGGCATGC AGAACCAGGA CCCAGTGGGA 
CAGGGGTACC TGGCTCACCA GCTCCTCGGC GACAGCATGC AGCACCCGGG GGCAGGCCGC 
CCCGGTCAGC AGATGCTTGG GCAGATTAGT GCTACCTCAC ACATCAACAT CTATCAAGGG 
CCAGAGAGCT GCCTGCCAGG GGCTCACGGC ATGGGCAGCC AGCCGTCAAG CTTGGCAGTT 
GTCAGGGGCT ACCAGCCATG TGCCAGCTTT GGGGGCAGCA GGCGCCAGGC TATGCCGAGG 
GACAGCCTTG CTCTGCAGTC AGGACAGCTC AGTGACACAA GTCAGACCTG CAGGGTGAAT 
GGCATCAAGA TGGAGATGAA AGGGCAGCCC CATCCGCTGT GCTCTAATCT GCAGAATTAC 
TCTGGTCAGT TCTATGACCA AACCGTGGGC TTCAGTCAGC AAGACACGAA AGCTGGTTCA 
TTCTCTATTT CAGACGCCAG CTGCCTGCTA CAGGGGACCA GCGCCAAAAA CTCTGAGTTA 
CTTTCCCCAG GTGCTAATCA GGTGACAAGC ACAGTGGACA GCCTCGACAG CCATGACCTG 
GAAGGGGTAC AGATTGACTT CGATGCCATC ATAGACGATG GGGACCACTC CAGCCTGATG 
TCGGGGGCCC TGAGCCCAAG TATCATTCAG AACCTTTCCT ATAGCTCCTC CCGCCTCACC 
ACGCCTCGGG CGTCCCTCCC ATTCCCAGCG CTGTCCATGA GCACCACCAA CATGGCTATC 
GGGGACATGA GTTCTTTGCT GACCTCCCTA GCGGAAGAAA GCAAATTCCT TGCAGTTATG 
CAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812300.1
CDS	168..4733
Translation	MQPQNVQGLSKVSEEPSTSSDERASLIKKEIHGSLPHVAEPSVPYRGTVFAMDPRNGYMEPHYHPPHLFPAFHPPVPIDARHHEGRYHYDPSPIPPLHMTSALSSSPTYPDLPFIRISPHRNPAAASESPFSPPHPYINPYMDYIRSLHSSPSLSMISATRGLSPTDAPHAGVSPAEYYHQMALLTGQRSPYADIIPSAATAGTGAIHMEYLHAMDSTRFPSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYSSAPVSLHMHQQILSRQQSLGSAFGHSPPLIHPAPTFPTQRPIPGIPTVLNPVQVSSGPSESSQNKPTSESAVSSTGDPMHNKRSKIKPDEDLPSPGARGQQEQPEGTTLVKEEGDKDESKQEPEVIYETNCHWEGCAREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPRDSGSHSQSRSPGRPTQGALGEQQDLSNTTSKREECLQVKTVKAEKPMTSQPSPGGQSSCSSQQSPISNYSNSGLELPLTDGGSIGDLSAIDETPIMDSTISTATTALALQARRNPAGTKWMEHVKLERLKQVNGMFPRLNPILPPKAPAVSPLIGNGTQSNNTCSLGGPMTLLPGRSDLSGVDVTMLNMLNRRDSSASTISSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSEASQSDGLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRLALLGDALEPGVALPPVHAPRRCSDGGAHGYGRRHLQPHDAPGHGVRRASDPVRTGSEGLALPRVPRFSSLSSCNPPAMATSAEKRSLVLQNYTRPEGGQSRNFHSSPCPPSITENVTLESLTMDADANLNDEDFLPDDVVQYLNSQNQAGYEQHFPSALPDDSKVPHGPGDFDAPGLPDSHAGQQFHALEQPCPEGSKTDLPIQWNEVSSGSADLSSSKLKCGPRPAVPQTRAFGFCNGMVVHPQNPLRSGPAGGYQTLGENSNPYGGPEHLMLHNSPGSGTSGNAFHEQPCKAPQYGNCLNRQPVAPGALDGACGAGIQASKLKSTPMQGSGGQLNFGLPVAPNESAGSMVNGMQNQDPVGQGYLAHQLLGDSMQHPGAGRPGQQMLGQISATSHINIYQGPESCLPGAHGMGSQPSSLAVVRGYQPCASFGGSRRQAMPRDSLALQSGQLSDTSQTCRVNGIKMEMKGQPHPLCSNLQNYSGQFYDQTVGFSQQDTKAGSFSISDASCLLQGTSAKNSELLSPGANQVTSTVDSLDSHDLEGVQIDFDAIIDDGDHSSLMSGALSPSIIQNLSYSSSRLTTPRASLPFPALSMSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	XM_016956811.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956811.2

ORF Insert Sequence:

ATGCAGCCAC AGAATGTCCA GGGGCTCAGC AAAGTCAGTG AGGAACCTTC AACATCGAGT 
GACGAGAGGG CCTCATTGAT CAAGAAAGAG ATCCATGGGT CCCTGCCACA CGTGGCAGAG 
CCCTCTGTGC CGTACCGCGG GACGGTGTTT GCCATGGACC CCAGGAATGG TTACATGGAG 
CCCCACTACC ACCCTCCTCA TCTTTTCCCT GCCTTCCATC CTCCTGTACC AATTGATGCC 
AGACATCATG AGGGCCGTTA CCATTACGAT CCGTCTCCGA TTCCTCCATT GCATATGACT 
TCCGCCTTAT CTAGTAGCCC TACGTATCCG GACCTGCCCT TCATTAGGAT CTCCCCACAC 
CGGAACCCCG CTGCTGCTTC CGAGTCTCCC TTCAGCCCTC CACATCCCTA CATTAATCCC 
TACATGGACT ATATCCGCTC CTTGCACAGC AGCCCATCGC TCTCCATGAT CTCAGCAACC 
CGTGGGCTGA GCCCTACAGA TGCGCCCCAT GCAGGAGTCA GCCCAGCAGA ATACTATCAT 
CAGATGGCCC TGCTAACTGG CCAGCGCAGC CCCTATGCAG ACATTATTCC CTCGGCTGCC 
ACCGCCGGCA CGGGGGCCAT CCACATGGAA TATCTTCATG CTATGGATAG CACCAGATTC 
CCCAGCCCCA GGCTGTCAGC CAGGCCGAGC CGAAAACGTA CACTGTCCAT ATCACCACTG 
TCCGATCATA GCTTTGACCT TCAGACCATG ATAAGGACGT CTCCCAACTC CTTGGTCACG 
ATTCTCAATA ATTCCCGTAG CAGCTCTTCA GCAAGTGGCT CCTATGGTCA CTTATCTGCA 
AGTGCAATCA GCCCTGCCTT GAGCTTCACC TACTCTTCCG CGCCCGTCTC TCTCCACATG 
CATCAGCAGA TCCTAAGCCG ACAACAGAGC TTAGGTTCAG CCTTTGGACA CAGCCCTCCA 
CTCATCCACC CTGCCCCAAC TTTTCCAACA CAGAGGCCTA TTCCAGGGAT CCCTACGGTT 
CTGAACCCCG TCCAGGTCAG CTCCGGCCCT TCTGAGTCCT CACAGAACAA GCCCACGAGT 
GAGTCTGCAG TGAGTAGCAC TGGTGACCCG ATGCACAACA AGAGGTCCAA GATCAAACCC 
GATGAAGACC TCCCCAGCCC AGGGGCTCGG GGGCAGCAGG AACAGCCCGA AGGAACAACC 
CTTGTCAAGG AGGAAGGGGA CAAAGATGAA AGCAAACAGG AGCCTGAAGT CATCTATGAG 
ACAAACTGTC ACTGGGAAGG CTGCGCGAGG GAGTTCGACA CCCAAGAGCA GCTTGTGCAC 
CATATAAATA ATGACCATAT TCATGGAGAG AAGAAGGAGT TCGTGTGCAG GTGGCTGGAC 
TGCTCAAGAG AGCAGAAACC CTTCAAAGCC CAGTATATGT TGGTAGTGCA TATGAGAAGA 
CACACGGGCG AGAAGCCTCA CAAATGCACT TTTGAAGGTT GCACAAAGGC CTACTCGAGA 
CTAGAAAACT TGAAAACACA CTTGAGATCT CACACTGGAG AGAAACCATA CGTCTGTGAG 
CACGAAGGTT GCAACAAGGC TTTCTCAAAC GCCTCTGATC GCGCCAAACA CCAAAACAGA 
ACGCATTCCA ATGAGAAACC ATATGTGTGC AAAATCCCAG GCTGCACTAA GCGTTACACA 
GACCCAAGCT CCCTCCGGAA ACATGTGAAG ACAGTGCATG GCCCAGAGGC TCATGTCACC 
AAGAAGCAGC GAGGGGACAT CCATCCTCGG CCACCACCCC CGAGAGATTC CGGCAGCCAT 
TCACAGTCCA GGTCGCCCGG CCGACCGACT CAGGGAGCCC TTGGTGAGCA GCAGGACCTC 
AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAGGTGA AAACCGTCAA GGCAGAGAAG 
CCAATGACAT CTCAGCCAAG CCCTGGTGGT CAGTCTTCAT GCAGCAGCCA ACAGTCCCCC 
ATCAGCAACT ATTCCAACAG TGGGCTCGAG CTTCCTCTGA CCGATGGAGG TAGTATAGGA 
GACCTCAGTG CCATCGATGA AACCCCAATC ATGGACTCAA CCATTTCCAC TGCAACCACA 
GCCCTTGCTT TGCAAGCCAG GAGAAACCCG GCAGGGACCA AATGGATGGA GCACGTAAAA 
CTAGAAAGGC TAAAACAAGT GAATGGAATG TTTCCGCGAC TGAACCCCAT TCTACCCCCT 
AAAGCCCCTG CGGTCTCTCC TCTCATAGGA AATGGCACAC AGTCCAACAA CACCTGCAGC 
TTGGGTGGGC CCATGACGCT TCTCCCGGGC AGAAGCGACC TCTCTGGGGT GGACGTCACT 
ATGCTGAACA TGCTCAACAG AAGGGACAGC AGCGCCAGCA CCATCAGCTC GGCCTACCTG 
AGCAGCCGCC GCTCCTCAGG GATCTCGCCC TGCTTCTCCA GCCGCCGCTC CAGCGAGGCG 
TCACAGGCCG AGGGCCGGCC GCAGAACGTG AGCGTGGCCG ACTCCTACGA CCCCATCTCC 
ACCGACGCCT CGCGCCGCTC CAGCGAAGCC AGCCAGAGCG ACGGCCTGCC CAGCCTGCTC 
AGCCTCACGC CCGCCCAGCA GTACCGCCTC AAGGCCAAGT ACGCGGCTGC CACAGGAGGG 
CCGCCGCCCA CGCCCCTGCC CAACATGGAG AGGATGAGCC TGAAGACGCG CCTGGCGCTG 
CTCGGGGATG CCCTCGAGCC TGGCGTGGCC CTGCCTCCAG TTCATGCCCC GAGGAGGTGC 
AGCGACGGGG GAGCCCACGG CTACGGGCGG CGCCACCTGC AGCCGCACGA TGCGCCGGGC 
CACGGCGTGA GGAGGGCCAG CGACCCCGTG CGGACAGGCT CCGAGGGCCT GGCCCTGCCT 
CGTGTGCCGC GCTTCAGCAG CCTCAGCAGC TGCAACCCCC CGGCGATGGC CACGTCCGCG 
GAGAAGCGCA GTCTCGTGCT TCAGAATTAC ACGCGGCCCG AGGGCGGCCA GTCCCGAAAC 
TTCCACTCGT CCCCCTGTCC TCCCAGCATC ACCGAGAACG TCACCCTGGA GTCCCTGACC 
ATGGACGCTG ATGCCAACCT GAACGATGAG GATTTCCTGC CGGACGACGT GGTGCAGTAT 
TTAAATTCCC AGAACCAAGC AGGGTACGAG CAGCACTTCC CCAGCGCCCT CCCGGACGAC 
AGCAAAGTGC CCCACGGGCC CGGTGACTTT GACGCGCCCG GGCTGCCAGA CAGCCACGCT 
GGCCAGCAGT TCCATGCCCT CGAGCAGCCC TGCCCCGAGG GCAGCAAAAC CGACCTGCCC 
ATTCAGTGGA ACGAAGTCAG CTCCGGAAGC GCCGACCTGT CCTCCTCCAA GCTCAAGTGT 
GGGCCGCGGC CCGCTGTGCC GCAGACTCGC GCCTTTGGGT TCTGCAACGG CATGGTCGTC 
CACCCGCAGA ACCCCTTGAG GAGCGGGCCT GCTGGGGGCT ATCAGACCCT CGGGGAGAAC 
AGCAACCCCT ACGGTGGCCC AGAGCACTTG ATGCTCCACA ACAGCCCCGG AAGTGGCACC 
AGTGGAAACG CCTTCCATGA ACAGCCCTGT AAGGCCCCGC AGTATGGGAA CTGTCTCAAC 
AGGCAGCCAG TGGCCCCTGG TGCACTCGAC GGTGCCTGTG GTGCCGGGAT TCAAGCCTCA 
AAGCTGAAGA GCACCCCCAT GCAAGGGAGC GGGGGCCAGC TGAATTTCGG CCTGCCGGTA 
GCGCCAAATG AGTCAGCTGG CAGCATGGTG AATGGCATGC AGAACCAGGA CCCAGTGGGA 
CAGGGGTACC TGGCTCACCA GCTCCTCGGC GACAGCATGC AGCACCCGGG GGCAGGCCGC 
CCCGGTCAGC AGATGCTTGG GCAGATTAGT GCTACCTCAC ACATCAACAT CTATCAAGGG 
CCAGAGAGCT GCCTGCCAGG GGCTCACGGC ATGGGCAGCC AGCCGTCAAG CTTGGCAGTT 
GTCAGGGGCT ACCAGCCATG TGCCAGCTTT GGGGGCAGCA GGCGCCAGGC TATGCCGAGG 
GACAGCCTTG CTCTGCAGTC AGGACAGCTC AGTGACACAA GTCAGACCTG CAGGGTGAAT 
GGCATCAAGA TGGAGATGAA AGGGCAGCCC CATCCGCTGT GCTCTAATCT GCAGAATTAC 
TCTGGTCAGT TCTATGACCA AACCGTGGGC TTCAGTCAGC AAGACACGAA AGCTGGTTCA 
TTCTCTATTT CAGACGCCAG CTGCCTGCTA CAGGGGACCA GCGCCAAAAA CTCTGAGTTA 
CTTTCCCCAG GTGCTAATCA GGTGACAAGC ACAGTGGACA GCCTCGACAG CCATGACCTG 
GAAGGGGTAC AGATTGACTT CGATGCCATC ATAGACGATG GGGACCACTC CAGCCTGATG 
TCGGGGGCCC TGAGCCCAAG TATCATTCAG AACCTTTCCT ATAGCTCCTC CCGCCTCACC 
ACGCCTCGGG CGTCCCTCCC ATTCCCAGCG CTGTCCATGA GCACCACCAA CATGGCTATC 
GGGGACATGA GTTCTTTGCT GACCTCCCTA GCGGAAGAAA GCAAATTCCT TGCAGTTATG 
CAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI3 ( XM_016956811.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812300.1 Pan troglodytes GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.

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CloneID	OPc56900
Clone ID Related Accession (Same CDS sequence)	XM_016956811.1
Accession Version	XM_016956811.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	transcriptional activator GLI3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006474.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCAC AGAATGTCCA GGGGCTCAGC AAAGTCAGTG AGGAACCTTC AACATCGAGT 
GACGAGAGGG CCTCATTGAT CAAGAAAGAG ATCCATGGGT CCCTGCCACA CGTGGCAGAG 
CCCTCTGTGC CGTACCGCGG GACGGTGTTT GCCATGGACC CCAGGAATGG TTACATGGAG 
CCCCACTACC ACCCTCCTCA TCTTTTCCCT GCCTTCCATC CTCCTGTACC AATTGATGCC 
AGACATCATG AGGGCCGTTA CCATTACGAT CCGTCTCCGA TTCCTCCATT GCATATGACT 
TCCGCCTTAT CTAGTAGCCC TACGTATCCG GACCTGCCCT TCATTAGGAT CTCCCCACAC 
CGGAACCCCG CTGCTGCTTC CGAGTCTCCC TTCAGCCCTC CACATCCCTA CATTAATCCC 
TACATGGACT ATATCCGCTC CTTGCACAGC AGCCCATCGC TCTCCATGAT CTCAGCAACC 
CGTGGGCTGA GCCCTACAGA TGCGCCCCAT GCAGGAGTCA GCCCAGCAGA ATACTATCAT 
CAGATGGCCC TGCTAACTGG CCAGCGCAGC CCCTATGCAG ACATTATTCC CTCGGCTGCC 
ACCGCCGGCA CGGGGGCCAT CCACATGGAA TATCTTCATG CTATGGATAG CACCAGATTC 
CCCAGCCCCA GGCTGTCAGC CAGGCCGAGC CGAAAACGTA CACTGTCCAT ATCACCACTG 
TCCGATCATA GCTTTGACCT TCAGACCATG ATAAGGACGT CTCCCAACTC CTTGGTCACG 
ATTCTCAATA ATTCCCGTAG CAGCTCTTCA GCAAGTGGCT CCTATGGTCA CTTATCTGCA 
AGTGCAATCA GCCCTGCCTT GAGCTTCACC TACTCTTCCG CGCCCGTCTC TCTCCACATG 
CATCAGCAGA TCCTAAGCCG ACAACAGAGC TTAGGTTCAG CCTTTGGACA CAGCCCTCCA 
CTCATCCACC CTGCCCCAAC TTTTCCAACA CAGAGGCCTA TTCCAGGGAT CCCTACGGTT 
CTGAACCCCG TCCAGGTCAG CTCCGGCCCT TCTGAGTCCT CACAGAACAA GCCCACGAGT 
GAGTCTGCAG TGAGTAGCAC TGGTGACCCG ATGCACAACA AGAGGTCCAA GATCAAACCC 
GATGAAGACC TCCCCAGCCC AGGGGCTCGG GGGCAGCAGG AACAGCCCGA AGGAACAACC 
CTTGTCAAGG AGGAAGGGGA CAAAGATGAA AGCAAACAGG AGCCTGAAGT CATCTATGAG 
ACAAACTGCC ACTGGGAAGG CTGCGCGAGG GAGTTCGACA CCCAAGAGCA GCTTGTGCAC 
CATATAAATA ATGACCATAT TCATGGAGAG AAGAAGGAGT TCGTGTGCAG GTGGCTGGAC 
TGCTCAAGAG AGCAGAAACC CTTCAAAGCC CAGTATATGT TGGTAGTGCA TATGAGAAGA 
CACACGGGCG AGAAGCCTCA CAAATGCACT TTTGAAGGTT GCACAAAGGC CTACTCGAGA 
CTAGAAAACT TGAAAACACA CTTGAGATCT CACACTGGAG AGAAACCATA CGTCTGTGAG 
CACGAAGGTT GCAACAAGGC TTTCTCAAAC GCCTCTGATC GCGCCAAACA CCAAAACAGA 
ACGCATTCCA ATGAGAAACC ATATGTGTGC AAAATCCCAG GCTGCACTAA GCGTTACACA 
GACCCAAGCT CCCTCCGGAA ACATGTGAAG ACAGTGCATG GCCCAGAGGC TCATGTCACC 
AAGAAGCAGC GAGGGGACAT CCATCCTCGG CCACCACCCC CGAGAGATTC CGGCAGCCAT 
TCACAGTCCA GGTCGCCCGG CCGACCGACT CAGGGAGCCC TTGGTGAGCA GCAGGACCTC 
AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAGGTGA AAACCGTCAA GGCAGAGAAG 
CCAATGACAT CTCAGCCAAG CCCTGGTGGT CAGTCTTCAT GCAGCAGCCA ACAGTCCCCC 
ATCAGCAACT ATTCCAACAG TGGGCTCGAG CTTCCTCTGA CCGATGGAGG TAGTATAGGA 
GACCTCAGTG CCATCGATGA AACCCCAATC ATGGACTCAA CCATTTCCAC TGCAACCACA 
GCCCTTGCTT TGCAAGCCAG GAGAAACCCG GCAGGGACCA AATGGATGGA GCACGTAAAA 
CTAGAAAGGC TAAAACAAGT GAATGGAATG TTTCCGCGAC TGAACCCCAT TCTACCCCCT 
AAAGCCCCTG CGGTCTCTCC TCTCATAGGA AATGGCACAC AGTCCAACAA CACCTGCAGC 
TTGGGTGGGC CCATGACGCT TCTCCCGGGC AGAAGCGACC TCTCTGGGGT GGACGTCACT 
ATGCTGAACA TGCTCAACAG AAGGGACAGC AGCGCCAGCA CCATCAGCTC GGCCTACCTG 
AGCAGCCGCC GCTCCTCAGG GATCTCGCCC TGCTTCTCCA GCCGCCGCTC CAGCGAGGCG 
TCACAGGCCG AGGGCCGGCC GCAGAACGTG AGCGTGGCCG ACTCCTACGA CCCCATCTCC 
ACCGACGCCT CGCGCCGCTC CAGCGAAGCC AGCCAGAGCG ACGGCCTGCC CAGCCTGCTC 
AGCCTCACGC CCGCCCAGCA GTACCGCCTC AAGGCCAAGT ACGCGGCTGC CACAGGAGGG 
CCGCCGCCCA CGCCCCTGCC CAACATGGAG AGGATGAGCC TGAAGACGCG CCTGGCGCTG 
CTCGGGGATG CCCTCGAGCC TGGCGTGGCC CTGCCTCCAG TTCATGCCCC GAGGAGGTGC 
AGCGACGGGG GAGCCCACGG CTACGGGCGG CGCCACCTGC AGCCGCACGA TGCGCCGGGC 
CACGGCGTGA GGAGGGCCAG CGACCCCGTG CGGACAGGCT CCGAGGGCCT GGCCCTGCCT 
CGTGTGCCGC GCTTCAGCAG CCTCAGCAGC TGCAACCCCC CGGCGATGGC CACGTCCGCG 
GAGAAGCGCA GTCTCGTGCT TCAGAATTAC ACGCGGCCCG AGGGCGGCCA GTCCCGAAAC 
TTCCACTCGT CCCCCTGTCC TCCCAGCATC ACCGAGAACG TCACCCTGGA GTCCCTGACC 
ATGGACGCTG ATGCCAACCT GAACGATGAG GATTTCCTGC CGGACGACGT GGTGCAGTAT 
TTAAATTCCC AGAACCAAGC AGGGTACGAG CAGCACTTCC CCAGCGCCCT CCCGGACGAC 
AGCAAAGTGC CCCACGGGCC CGGTGACTTT GACGCGCCCG GGCTGCCAGA CAGCCACGCT 
GGCCAGCAGT TCCATGCCCT CGAGCAGCCC TGCCCCGAGG GCAGCAAAAC CGACCTGCCC 
ATTCAGTGGA ACGAAGTCAG CTCCGGAAGC GCCGACCTGT CCTCCTCCAA GCTCAAGTGT 
GGGCCGCGGC CCGCTGTGCC GCAGACTCGC GCCTTTGGGT TCTGCAACGG CATGGTCGTC 
CACCCGCAGA ACCCCTTGAG GAGCGGGCCT GCTGGGGGCT ATCAGACCCT CGGGGAGAAC 
AGCAACCCCT ACGGTGGCCC AGAGCACTTG ATGCTCCACA ACAGCCCCGG AAGTGGCACC 
AGTGGAAACG CCTTCCATGA ACAGCCCTGT AAGGCCCCGC AGTATGGGAA CTGTCTCAAC 
AGGCAGCCAG TGGCCCCTGG TGCACTCGAC GGTGCCTGTG GTGCCGGGAT TCAAGCCTCA 
AAGCTGAAGA GCACCCCCAT GCAAGGGAGC GGGGGCCAGC TGAATTTCGG CCTGCCGGTA 
GCGCCAAATG AGTCAGCTGG CAGCATGGTG AATGGCATGC AGAACCAGGA CCCAGTGGGA 
CAGGGGTACC TGGCTCACCA GCTCCTCGGC GACAGCATGC AGCACCCGGG GGCAGGCCGC 
CCCGGTCAGC AGATGCTTGG GCAGATTAGT GCTACCTCAC ACATCAACAT CTATCAAGGG 
CCAGAGAGCT GCCTGCCAGG GGCTCACGGC ATGGGCAGCC AGCCGTCAAG CTTGGCAGTT 
GTCAGGGGCT ACCAGCCATG TGCCAGCTTT GGGGGCAGCA GGCGCCAGGC TATGCCGAGG 
GACAGCCTTG CTCTGCAGTC AGGACAGCTC AGTGACACAA GTCAGACCTG CAGGGTGAAT 
GGCATCAAGA TGGAGATGAA AGGGCAGCCC CATCCGCTGT GCTCTAATCT GCAGAATTAC 
TCTGGTCAGT TCTATGACCA AACCGTGGGC TTCAGTCAGC AAGACACGAA AGCTGGTTCA 
TTCTCTATTT CAGACGCCAG CTGCCTGCTA CAGGGGACCA GCGCCAAAAA CTCTGAGTTA 
CTTTCCCCAG GTGCTAATCA GGTGACAAGC ACAGTGGACA GCCTCGACAG CCATGACCTG 
GAAGGGGTAC AGATTGACTT CGATGCCATC ATAGACGATG GGGACCACTC CAGCCTGATG 
TCGGGGGCCC TGAGCCCAAG TATCATTCAG AACCTTTCCT ATAGCTCCTC CCGCCTCACC 
ACGCCTCGGG CGTCCCTCCC ATTCCCAGCG CTGTCCATGA GCACCACCAA CATGGCTATC 
GGGGACATGA GTTCTTTGCT GACCTCCCTA GCGGAAGAAA GCAAATTCCT TGCAGTTATG 
CAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812300.1
CDS	174..4739
Translation	MQPQNVQGLSKVSEEPSTSSDERASLIKKEIHGSLPHVAEPSVPYRGTVFAMDPRNGYMEPHYHPPHLFPAFHPPVPIDARHHEGRYHYDPSPIPPLHMTSALSSSPTYPDLPFIRISPHRNPAAASESPFSPPHPYINPYMDYIRSLHSSPSLSMISATRGLSPTDAPHAGVSPAEYYHQMALLTGQRSPYADIIPSAATAGTGAIHMEYLHAMDSTRFPSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYSSAPVSLHMHQQILSRQQSLGSAFGHSPPLIHPAPTFPTQRPIPGIPTVLNPVQVSSGPSESSQNKPTSESAVSSTGDPMHNKRSKIKPDEDLPSPGARGQQEQPEGTTLVKEEGDKDESKQEPEVIYETNCHWEGCAREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPRDSGSHSQSRSPGRPTQGALGEQQDLSNTTSKREECLQVKTVKAEKPMTSQPSPGGQSSCSSQQSPISNYSNSGLELPLTDGGSIGDLSAIDETPIMDSTISTATTALALQARRNPAGTKWMEHVKLERLKQVNGMFPRLNPILPPKAPAVSPLIGNGTQSNNTCSLGGPMTLLPGRSDLSGVDVTMLNMLNRRDSSASTISSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSEASQSDGLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRLALLGDALEPGVALPPVHAPRRCSDGGAHGYGRRHLQPHDAPGHGVRRASDPVRTGSEGLALPRVPRFSSLSSCNPPAMATSAEKRSLVLQNYTRPEGGQSRNFHSSPCPPSITENVTLESLTMDADANLNDEDFLPDDVVQYLNSQNQAGYEQHFPSALPDDSKVPHGPGDFDAPGLPDSHAGQQFHALEQPCPEGSKTDLPIQWNEVSSGSADLSSSKLKCGPRPAVPQTRAFGFCNGMVVHPQNPLRSGPAGGYQTLGENSNPYGGPEHLMLHNSPGSGTSGNAFHEQPCKAPQYGNCLNRQPVAPGALDGACGAGIQASKLKSTPMQGSGGQLNFGLPVAPNESAGSMVNGMQNQDPVGQGYLAHQLLGDSMQHPGAGRPGQQMLGQISATSHINIYQGPESCLPGAHGMGSQPSSLAVVRGYQPCASFGGSRRQAMPRDSLALQSGQLSDTSQTCRVNGIKMEMKGQPHPLCSNLQNYSGQFYDQTVGFSQQDTKAGSFSISDASCLLQGTSAKNSELLSPGANQVTSTVDSLDSHDLEGVQIDFDAIIDDGDHSSLMSGALSPSIIQNLSYSSSRLTTPRASLPFPALSMSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	XM_016956811.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956811.1

ORF Insert Sequence:

ATGCAGCCAC AGAATGTCCA GGGGCTCAGC AAAGTCAGTG AGGAACCTTC AACATCGAGT 
GACGAGAGGG CCTCATTGAT CAAGAAAGAG ATCCATGGGT CCCTGCCACA CGTGGCAGAG 
CCCTCTGTGC CGTACCGCGG GACGGTGTTT GCCATGGACC CCAGGAATGG TTACATGGAG 
CCCCACTACC ACCCTCCTCA TCTTTTCCCT GCCTTCCATC CTCCTGTACC AATTGATGCC 
AGACATCATG AGGGCCGTTA CCATTACGAT CCGTCTCCGA TTCCTCCATT GCATATGACT 
TCCGCCTTAT CTAGTAGCCC TACGTATCCG GACCTGCCCT TCATTAGGAT CTCCCCACAC 
CGGAACCCCG CTGCTGCTTC CGAGTCTCCC TTCAGCCCTC CACATCCCTA CATTAATCCC 
TACATGGACT ATATCCGCTC CTTGCACAGC AGCCCATCGC TCTCCATGAT CTCAGCAACC 
CGTGGGCTGA GCCCTACAGA TGCGCCCCAT GCAGGAGTCA GCCCAGCAGA ATACTATCAT 
CAGATGGCCC TGCTAACTGG CCAGCGCAGC CCCTATGCAG ACATTATTCC CTCGGCTGCC 
ACCGCCGGCA CGGGGGCCAT CCACATGGAA TATCTTCATG CTATGGATAG CACCAGATTC 
CCCAGCCCCA GGCTGTCAGC CAGGCCGAGC CGAAAACGTA CACTGTCCAT ATCACCACTG 
TCCGATCATA GCTTTGACCT TCAGACCATG ATAAGGACGT CTCCCAACTC CTTGGTCACG 
ATTCTCAATA ATTCCCGTAG CAGCTCTTCA GCAAGTGGCT CCTATGGTCA CTTATCTGCA 
AGTGCAATCA GCCCTGCCTT GAGCTTCACC TACTCTTCCG CGCCCGTCTC TCTCCACATG 
CATCAGCAGA TCCTAAGCCG ACAACAGAGC TTAGGTTCAG CCTTTGGACA CAGCCCTCCA 
CTCATCCACC CTGCCCCAAC TTTTCCAACA CAGAGGCCTA TTCCAGGGAT CCCTACGGTT 
CTGAACCCCG TCCAGGTCAG CTCCGGCCCT TCTGAGTCCT CACAGAACAA GCCCACGAGT 
GAGTCTGCAG TGAGTAGCAC TGGTGACCCG ATGCACAACA AGAGGTCCAA GATCAAACCC 
GATGAAGACC TCCCCAGCCC AGGGGCTCGG GGGCAGCAGG AACAGCCCGA AGGAACAACC 
CTTGTCAAGG AGGAAGGGGA CAAAGATGAA AGCAAACAGG AGCCTGAAGT CATCTATGAG 
ACAAACTGCC ACTGGGAAGG CTGCGCGAGG GAGTTCGACA CCCAAGAGCA GCTTGTGCAC 
CATATAAATA ATGACCATAT TCATGGAGAG AAGAAGGAGT TCGTGTGCAG GTGGCTGGAC 
TGCTCAAGAG AGCAGAAACC CTTCAAAGCC CAGTATATGT TGGTAGTGCA TATGAGAAGA 
CACACGGGCG AGAAGCCTCA CAAATGCACT TTTGAAGGTT GCACAAAGGC CTACTCGAGA 
CTAGAAAACT TGAAAACACA CTTGAGATCT CACACTGGAG AGAAACCATA CGTCTGTGAG 
CACGAAGGTT GCAACAAGGC TTTCTCAAAC GCCTCTGATC GCGCCAAACA CCAAAACAGA 
ACGCATTCCA ATGAGAAACC ATATGTGTGC AAAATCCCAG GCTGCACTAA GCGTTACACA 
GACCCAAGCT CCCTCCGGAA ACATGTGAAG ACAGTGCATG GCCCAGAGGC TCATGTCACC 
AAGAAGCAGC GAGGGGACAT CCATCCTCGG CCACCACCCC CGAGAGATTC CGGCAGCCAT 
TCACAGTCCA GGTCGCCCGG CCGACCGACT CAGGGAGCCC TTGGTGAGCA GCAGGACCTC 
AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAGGTGA AAACCGTCAA GGCAGAGAAG 
CCAATGACAT CTCAGCCAAG CCCTGGTGGT CAGTCTTCAT GCAGCAGCCA ACAGTCCCCC 
ATCAGCAACT ATTCCAACAG TGGGCTCGAG CTTCCTCTGA CCGATGGAGG TAGTATAGGA 
GACCTCAGTG CCATCGATGA AACCCCAATC ATGGACTCAA CCATTTCCAC TGCAACCACA 
GCCCTTGCTT TGCAAGCCAG GAGAAACCCG GCAGGGACCA AATGGATGGA GCACGTAAAA 
CTAGAAAGGC TAAAACAAGT GAATGGAATG TTTCCGCGAC TGAACCCCAT TCTACCCCCT 
AAAGCCCCTG CGGTCTCTCC TCTCATAGGA AATGGCACAC AGTCCAACAA CACCTGCAGC 
TTGGGTGGGC CCATGACGCT TCTCCCGGGC AGAAGCGACC TCTCTGGGGT GGACGTCACT 
ATGCTGAACA TGCTCAACAG AAGGGACAGC AGCGCCAGCA CCATCAGCTC GGCCTACCTG 
AGCAGCCGCC GCTCCTCAGG GATCTCGCCC TGCTTCTCCA GCCGCCGCTC CAGCGAGGCG 
TCACAGGCCG AGGGCCGGCC GCAGAACGTG AGCGTGGCCG ACTCCTACGA CCCCATCTCC 
ACCGACGCCT CGCGCCGCTC CAGCGAAGCC AGCCAGAGCG ACGGCCTGCC CAGCCTGCTC 
AGCCTCACGC CCGCCCAGCA GTACCGCCTC AAGGCCAAGT ACGCGGCTGC CACAGGAGGG 
CCGCCGCCCA CGCCCCTGCC CAACATGGAG AGGATGAGCC TGAAGACGCG CCTGGCGCTG 
CTCGGGGATG CCCTCGAGCC TGGCGTGGCC CTGCCTCCAG TTCATGCCCC GAGGAGGTGC 
AGCGACGGGG GAGCCCACGG CTACGGGCGG CGCCACCTGC AGCCGCACGA TGCGCCGGGC 
CACGGCGTGA GGAGGGCCAG CGACCCCGTG CGGACAGGCT CCGAGGGCCT GGCCCTGCCT 
CGTGTGCCGC GCTTCAGCAG CCTCAGCAGC TGCAACCCCC CGGCGATGGC CACGTCCGCG 
GAGAAGCGCA GTCTCGTGCT TCAGAATTAC ACGCGGCCCG AGGGCGGCCA GTCCCGAAAC 
TTCCACTCGT CCCCCTGTCC TCCCAGCATC ACCGAGAACG TCACCCTGGA GTCCCTGACC 
ATGGACGCTG ATGCCAACCT GAACGATGAG GATTTCCTGC CGGACGACGT GGTGCAGTAT 
TTAAATTCCC AGAACCAAGC AGGGTACGAG CAGCACTTCC CCAGCGCCCT CCCGGACGAC 
AGCAAAGTGC CCCACGGGCC CGGTGACTTT GACGCGCCCG GGCTGCCAGA CAGCCACGCT 
GGCCAGCAGT TCCATGCCCT CGAGCAGCCC TGCCCCGAGG GCAGCAAAAC CGACCTGCCC 
ATTCAGTGGA ACGAAGTCAG CTCCGGAAGC GCCGACCTGT CCTCCTCCAA GCTCAAGTGT 
GGGCCGCGGC CCGCTGTGCC GCAGACTCGC GCCTTTGGGT TCTGCAACGG CATGGTCGTC 
CACCCGCAGA ACCCCTTGAG GAGCGGGCCT GCTGGGGGCT ATCAGACCCT CGGGGAGAAC 
AGCAACCCCT ACGGTGGCCC AGAGCACTTG ATGCTCCACA ACAGCCCCGG AAGTGGCACC 
AGTGGAAACG CCTTCCATGA ACAGCCCTGT AAGGCCCCGC AGTATGGGAA CTGTCTCAAC 
AGGCAGCCAG TGGCCCCTGG TGCACTCGAC GGTGCCTGTG GTGCCGGGAT TCAAGCCTCA 
AAGCTGAAGA GCACCCCCAT GCAAGGGAGC GGGGGCCAGC TGAATTTCGG CCTGCCGGTA 
GCGCCAAATG AGTCAGCTGG CAGCATGGTG AATGGCATGC AGAACCAGGA CCCAGTGGGA 
CAGGGGTACC TGGCTCACCA GCTCCTCGGC GACAGCATGC AGCACCCGGG GGCAGGCCGC 
CCCGGTCAGC AGATGCTTGG GCAGATTAGT GCTACCTCAC ACATCAACAT CTATCAAGGG 
CCAGAGAGCT GCCTGCCAGG GGCTCACGGC ATGGGCAGCC AGCCGTCAAG CTTGGCAGTT 
GTCAGGGGCT ACCAGCCATG TGCCAGCTTT GGGGGCAGCA GGCGCCAGGC TATGCCGAGG 
GACAGCCTTG CTCTGCAGTC AGGACAGCTC AGTGACACAA GTCAGACCTG CAGGGTGAAT 
GGCATCAAGA TGGAGATGAA AGGGCAGCCC CATCCGCTGT GCTCTAATCT GCAGAATTAC 
TCTGGTCAGT TCTATGACCA AACCGTGGGC TTCAGTCAGC AAGACACGAA AGCTGGTTCA 
TTCTCTATTT CAGACGCCAG CTGCCTGCTA CAGGGGACCA GCGCCAAAAA CTCTGAGTTA 
CTTTCCCCAG GTGCTAATCA GGTGACAAGC ACAGTGGACA GCCTCGACAG CCATGACCTG 
GAAGGGGTAC AGATTGACTT CGATGCCATC ATAGACGATG GGGACCACTC CAGCCTGATG 
TCGGGGGCCC TGAGCCCAAG TATCATTCAG AACCTTTCCT ATAGCTCCTC CCGCCTCACC 
ACGCCTCGGG CGTCCCTCCC ATTCCCAGCG CTGTCCATGA GCACCACCAA CATGGCTATC 
GGGGACATGA GTTCTTTGCT GACCTCCCTA GCGGAAGAAA GCAAATTCCT TGCAGTTATG 
CAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI3 ( NM_001034190.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> NP_001029362.1 Pan troglodytes GLI family zinc finger 3 (GLI3), mRNA.

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CloneID	OPc00006
Clone ID Related Accession (Same CDS sequence)	NM_001034190.1
Accession Version	NM_001034190.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Pan troglodytes(chimpanzee)
Product	transcriptional activator GLI3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY665272.1. On or before Oct 17, 2005 this sequence version replaced XM_519061.1, XM_519062.1. ##Evidence-Data-START## Transcript exon combination :: AY665272.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01120800, SAMN01120804 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGCCC AGTCCCACAG CTCCACGACC ACTGAAAAGA AAAAAGTTGA GAATTCCATA 
GTGAAGTGCT CCACTCGAAC AGATGTGAGC GAGAAAGCCG TTGCCTCCAG CACCACTTCT 
AATGAGGATG AAAGTCCTGG ACAGACTTAT CACAGAGAGA GAAGAAACGC AATCACTATG 
CAGCCACAGA ATGTCCAGGG GCTCAGCAAA GTCAGTGAGG AACCTTCAAC ATCGAGTGAC 
GAGAGGGCCT CATTGATCAA GAAAGAGATC CATGGGTCCC TGCCACACGT GGCAGAGCCC 
TCTGTGCCGT ACCGCGGGAC GGTGTTTGCC ATGGACCCCA GGAATGGTTA CATGGAGCCC 
CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA 
CATCATGAGG GCCGTTACCA TTACGATCCG TCTCCGATTC CTCCATTGCA TATGACTTCC 
GCCTTATCTA GTAGCCCTAC GTATCCGGAC CTGCCCTTCA TTAGGATCTC CCCACACCGG 
AACCCCGCTG CTGCTTCCGA GTCTCCCTTC AGCCCTCCAC ATCCCTACAT TAATCCCTAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCGCTCT CCATGATCTC AGCAACCCGT 
GGGCTGAGCC CTACAGATGC GCCCCATGCA GGAGTCAGCC CAGCAGAATA CTATCATCAG 
ATGGCCCTGC TAACTGGCCA GCGCAGCCCC TATGCAGACA TTATTCCCTC GGCTGCCACC 
GCCGGCACGG GGGCCATCCA CATGGAATAT CTTCATGCTA TGGATAGCAC CAGATTCCCC 
AGCCCCAGGC TGTCAGCCAG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTGTCC 
GATCATAGCT TTGACCTTCA GACCATGATA AGGACGTCTC CCAACTCCTT GGTCACGATT 
CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGCTCCT ATGGTCACTT ATCTGCAAGT 
GCAATCAGCC CTGCCTTGAG CTTCACCTAC TCTTCCGCGC CCGTCTCTCT CCACATGCAT 
CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGTTCAGCCT TTGGACACAG CCCTCCACTC 
ATCCACCCTG CCCCAACTTT TCCAACACAG AGGCCTATTC CAGGGATCCC TACGGTTCTG 
AACCCCGTCC AGGTCAGCTC CGGCCCTTCT GAGTCCTCAC AGAACAAGCC CACGAGTGAG 
TCTGCAGTGA GTAGCACTGG TGACCCGATG CACAACAAGA GGTCCAAGAT CAAACCCGAT 
GAAGACCTCC CCAGCCCAGG GGCTCGGGGG CAGCAGGAAC AGCCCGAAGG AACAACCCTT 
GTCAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CTGAAGTCAT CTATGAGACA 
AACTGCCACT GGGAAGGCTG CGCGAGGGAG TTCGACACCC AAGAGCAGCT TGTGCACCAT 
ATAAATAATG ACCATATTCA TGGAGAGAAG AAGGAGTTCG TGTGCAGGTG GCTGGACTGC 
TCAAGAGAGC AGAAACCCTT CAAAGCCCAG TATATGTTGG TAGTGCATAT GAGAAGACAC 
ACGGGCGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTA 
GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC 
GAAGGTTGCA ACAAGGCTTT CTCAAACGCC TCTGATCGCG CCAAACACCA AAACAGAACG 
CATTCCAATG AGAAACCATA TGTGTGCAAA ATCCCAGGCT GCACTAAGCG TTACACAGAC 
CCAAGCTCCC TCCGGAAACA TGTGAAGACA GTGCATGGCC CAGAGGCTCA TGTCACCAAG 
AAGCAGCGAG GGGACATCCA TCCTCGGCCA CCACCCCCGA GAGATTCCGG CAGCCATTCA 
CAGTCCAGGT CGCCCGGCCG ACCGACTCAG GGAGCCCTTG GTGAGCAGCA GGACCTCAGC 
AACACTACCT CAAAGCGGGA AGAATGCCTC CAGGTGAAAA CCGTCAAGGC AGAGAAGCCA 
ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC 
AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTAG TATAGGAGAC 
CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCAACCA TTTCCACTGC AACCACAGCC 
CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CGTAAAACTA 
GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCGCGACTGA ACCCCATTCT ACCCCCTAAA 
GCCCCTGCGG TCTCTCCTCT CATAGGAAAT GGCACACAGT CCAACAACAC CTGCAGCTTG 
GGTGGGCCCA TGACGCTTCT CCCGGGCAGA AGCGACCTCT CTGGGGTGGA CGTCACTATG 
CTGAACATGC TCAACAGAAG GGACAGCAGC GCCAGCACCA TCAGCTCGGC CTACCTGAGC 
AGCCGCCGCT CCTCAGGGAT CTCGCCCTGC TTCTCCAGCC GCCGCTCCAG CGAGGCGTCA 
CAGGCCGAGG GCCGGCCGCA GAACGTGAGC GTGGCCGACT CCTACGACCC CATCTCCACC 
GACGCCTCGC GCCGCTCCAG CGAAGCCAGC CAGAGCGACG GCCTGCCCAG CCTGCTCAGC 
CTCACGCCCG CCCAGCAGTA CCGCCTCAAG GCCAAGTACG CGGCTGCCAC AGGAGGGCCG 
CCGCCCACGC CCCTGCCCAA CATGGAGAGG ATGAGCCTGA AGACGCGCCT GGCGCTGCTC 
GGGGATGCCC TCGAGCCTGG CGTGGCCCTG CCTCCAGTTC ATGCCCCGAG GAGGTGCAGC 
GACGGGGGAG CCCACGGCTA CGGGCGGCGC CACCTGCAGC CGCACGATGC GCCGGGCCAC 
GGCGTGAGGA GGGCCAGCGA CCCCGTGCGG ACAGGCTCCG AGGGCCTGGC CCTGCCTCGT 
GTGCCGCGCT TCAGCAGCCT CAGCAGCTGC AACCCCCCGG CGATGGCCAC GTCCGCGGAG 
AAGCGCAGTC TCGTGCTTCA GAATTACACG CGGCCCGAGG GCGGCCAGTC CCGAAACTTC 
CACTCGTCCC CCTGTCCTCC CAGCATCACC GAGAACGTCA CCCTGGAGTC CCTGACCATG 
GACGCTGATG CCAACCTGAA CGATGAGGAT TTCCTGCCGG ACGACGTGGT GCAGTATTTA 
AATTCCCAGA ACCAAGCAGG GTACGAGCAG CACTTCCCCA GCACCCTCCC GGACGACAGC 
AAAGTGCCCC ACGGGCCCGG TGACTTTGAC GCGCCCGGGC TGCCAGACAG CCACGCTGGC 
CAGCAGTTCC ATGCCCTCGA GCAGCCCTGC CCCGAGGGCA GCAAAACCGA CCTGCCCATT 
CAGTGGAACG AAGTCAGCTC CGGAAGCGCC GACCTGTCCT CCTCCAAGCT CAAGTGTGGG 
CCGCGGCCCG CTGTGCCGCA GACTCGCGCC TTTGGGTTCT GCAACGGCAT GGTCGTCCAC 
CCGCAGAACC CCTTGAGGAG CGGGCCTGCT GGGGGCTATC AGACCCTCGG GGAGAACAGC 
AACCCCTACG GTGGCCCAGA GCACTTGATG CTCCACAACA GCCCCGGAAG TGGCACCAGT 
GGAAACGCCT TCCATGAACA GCCCTGTAAG GCCCCGCAGT ATGGGAACTG TCTCAACAGG 
CAGCCAGTGG CCCCTGGTGC ACTCGACGGT GCCTGTGGTG CCGGGATTCA AGCCTCAAAG 
CTGAAGAGCA CCCCCATGCA AGGGAGCGGG GGCCAGCTGA ATTTCGGCCT GCCGGTAGCG 
CCAAATGAGT CAGCTGGCAG CATGGTGAAT GGCATGCAGA ACCAGGACCC AGTGGGACAG 
GGGTACCTGG CTCACCAGCT CCTCGGCGAC AGCATGCAGC ACCCGGGGGC AGGCCGCCCC 
GGTCAGCAGA TGCTTGGGCA GATTAGTGCT ACCTCACACA TCAACATCTA TCAAGGGCCA 
GAGAGCTGCC TGCCAGGGGC TCACGGCATG GGCAGCCAGC CGTCAAGCTT GGCAGTTGTC 
AGGGGCTACC AGCCATGTGC CAGCTTTGGG GGCAGCAGGC GCCAGGCTAT GCCGAGGGAC 
AGCCTTGCTC TGCAGTCAGG ACAGCTCAGT GACACAAGTC AGACCTGCAG GGTGAATGGC 
ATCAAGATGG AGATGAAAGG GCAGCCCCAT CCGCTGTGCT CTAATCTGCA GAATTACTCT 
GGTCAGTTCT ATGACCAAAC CGTGGGCTTC AGTCAGCAAG ACACGAAAGC TGGTTCATTC 
TCTATTTCAG ACGCCAGCTG CCTGCTACAG GGGACCAGCG CCAAAAACTC TGAGTTACTT 
TCCCCAGGTG CTAATCAGGT GACAAGCACA GTGGACAGCC TCGACAGCCA TGACCTGGAA 
GGGGTACAGA TTGACTTCGA TGCCATCATA GACGATGGGG ACCACTCCAG CCTGATGTCG 
GGGGCCCTGA GCCCAAGTAT CATTCAGAAC CTTTCCTATA GCTCCTCCCG CCTCACCACG 
CCTCGGGCGT CCCTCCCATT CCCAGCGCTG TCCATGAGCA CCACCAACAT GGCTATCGGG 
GACATGAGTT CTTTGCTGAC CTCCCTAGCG GAAGAAAGCA AATTCCTTGC AGTTATGCAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001029362.1
CDS	1..4743
Translation	MEAQSHSSTTTEKKKVENSIVKCSTRTDVSEKAVASSTTSNEDESPGQTYHRERRNAITMQPQNVQGLSKVSEEPSTSSDERASLIKKEIHGSLPHVAEPSVPYRGTVFAMDPRNGYMEPHYHPPHLFPAFHPPVPIDARHHEGRYHYDPSPIPPLHMTSALSSSPTYPDLPFIRISPHRNPAAASESPFSPPHPYINPYMDYIRSLHSSPSLSMISATRGLSPTDAPHAGVSPAEYYHQMALLTGQRSPYADIIPSAATAGTGAIHMEYLHAMDSTRFPSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYSSAPVSLHMHQQILSRQQSLGSAFGHSPPLIHPAPTFPTQRPIPGIPTVLNPVQVSSGPSESSQNKPTSESAVSSTGDPMHNKRSKIKPDEDLPSPGARGQQEQPEGTTLVKEEGDKDESKQEPEVIYETNCHWEGCAREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPRDSGSHSQSRSPGRPTQGALGEQQDLSNTTSKREECLQVKTVKAEKPMTSQPSPGGQSSCSSQQSPISNYSNSGLELPLTDGGSIGDLSAIDETPIMDSTISTATTALALQARRNPAGTKWMEHVKLERLKQVNGMFPRLNPILPPKAPAVSPLIGNGTQSNNTCSLGGPMTLLPGRSDLSGVDVTMLNMLNRRDSSASTISSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSEASQSDGLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRLALLGDALEPGVALPPVHAPRRCSDGGAHGYGRRHLQPHDAPGHGVRRASDPVRTGSEGLALPRVPRFSSLSSCNPPAMATSAEKRSLVLQNYTRPEGGQSRNFHSSPCPPSITENVTLESLTMDADANLNDEDFLPDDVVQYLNSQNQAGYEQHFPSTLPDDSKVPHGPGDFDAPGLPDSHAGQQFHALEQPCPEGSKTDLPIQWNEVSSGSADLSSSKLKCGPRPAVPQTRAFGFCNGMVVHPQNPLRSGPAGGYQTLGENSNPYGGPEHLMLHNSPGSGTSGNAFHEQPCKAPQYGNCLNRQPVAPGALDGACGAGIQASKLKSTPMQGSGGQLNFGLPVAPNESAGSMVNGMQNQDPVGQGYLAHQLLGDSMQHPGAGRPGQQMLGQISATSHINIYQGPESCLPGAHGMGSQPSSLAVVRGYQPCASFGGSRRQAMPRDSLALQSGQLSDTSQTCRVNGIKMEMKGQPHPLCSNLQNYSGQFYDQTVGFSQQDTKAGSFSISDASCLLQGTSAKNSELLSPGANQVTSTVDSLDSHDLEGVQIDFDAIIDDGDHSSLMSGALSPSIIQNLSYSSSRLTTPRASLPFPALSMSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	NM_001034190.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes GLI family zinc finger 3 (GLI3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001034190.1

ORF Insert Sequence:

ATGGAGGCCC AGTCCCACAG CTCCACGACC ACTGAAAAGA AAAAAGTTGA GAATTCCATA 
GTGAAGTGCT CCACTCGAAC AGATGTGAGC GAGAAAGCCG TTGCCTCCAG CACCACTTCT 
AATGAGGATG AAAGTCCTGG ACAGACTTAT CACAGAGAGA GAAGAAACGC AATCACTATG 
CAGCCACAGA ATGTCCAGGG GCTCAGCAAA GTCAGTGAGG AACCTTCAAC ATCGAGTGAC 
GAGAGGGCCT CATTGATCAA GAAAGAGATC CATGGGTCCC TGCCACACGT GGCAGAGCCC 
TCTGTGCCGT ACCGCGGGAC GGTGTTTGCC ATGGACCCCA GGAATGGTTA CATGGAGCCC 
CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA 
CATCATGAGG GCCGTTACCA TTACGATCCG TCTCCGATTC CTCCATTGCA TATGACTTCC 
GCCTTATCTA GTAGCCCTAC GTATCCGGAC CTGCCCTTCA TTAGGATCTC CCCACACCGG 
AACCCCGCTG CTGCTTCCGA GTCTCCCTTC AGCCCTCCAC ATCCCTACAT TAATCCCTAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCGCTCT CCATGATCTC AGCAACCCGT 
GGGCTGAGCC CTACAGATGC GCCCCATGCA GGAGTCAGCC CAGCAGAATA CTATCATCAG 
ATGGCCCTGC TAACTGGCCA GCGCAGCCCC TATGCAGACA TTATTCCCTC GGCTGCCACC 
GCCGGCACGG GGGCCATCCA CATGGAATAT CTTCATGCTA TGGATAGCAC CAGATTCCCC 
AGCCCCAGGC TGTCAGCCAG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTGTCC 
GATCATAGCT TTGACCTTCA GACCATGATA AGGACGTCTC CCAACTCCTT GGTCACGATT 
CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGCTCCT ATGGTCACTT ATCTGCAAGT 
GCAATCAGCC CTGCCTTGAG CTTCACCTAC TCTTCCGCGC CCGTCTCTCT CCACATGCAT 
CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGTTCAGCCT TTGGACACAG CCCTCCACTC 
ATCCACCCTG CCCCAACTTT TCCAACACAG AGGCCTATTC CAGGGATCCC TACGGTTCTG 
AACCCCGTCC AGGTCAGCTC CGGCCCTTCT GAGTCCTCAC AGAACAAGCC CACGAGTGAG 
TCTGCAGTGA GTAGCACTGG TGACCCGATG CACAACAAGA GGTCCAAGAT CAAACCCGAT 
GAAGACCTCC CCAGCCCAGG GGCTCGGGGG CAGCAGGAAC AGCCCGAAGG AACAACCCTT 
GTCAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CTGAAGTCAT CTATGAGACA 
AACTGCCACT GGGAAGGCTG CGCGAGGGAG TTCGACACCC AAGAGCAGCT TGTGCACCAT 
ATAAATAATG ACCATATTCA TGGAGAGAAG AAGGAGTTCG TGTGCAGGTG GCTGGACTGC 
TCAAGAGAGC AGAAACCCTT CAAAGCCCAG TATATGTTGG TAGTGCATAT GAGAAGACAC 
ACGGGCGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTA 
GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC 
GAAGGTTGCA ACAAGGCTTT CTCAAACGCC TCTGATCGCG CCAAACACCA AAACAGAACG 
CATTCCAATG AGAAACCATA TGTGTGCAAA ATCCCAGGCT GCACTAAGCG TTACACAGAC 
CCAAGCTCCC TCCGGAAACA TGTGAAGACA GTGCATGGCC CAGAGGCTCA TGTCACCAAG 
AAGCAGCGAG GGGACATCCA TCCTCGGCCA CCACCCCCGA GAGATTCCGG CAGCCATTCA 
CAGTCCAGGT CGCCCGGCCG ACCGACTCAG GGAGCCCTTG GTGAGCAGCA GGACCTCAGC 
AACACTACCT CAAAGCGGGA AGAATGCCTC CAGGTGAAAA CCGTCAAGGC AGAGAAGCCA 
ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC 
AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTAG TATAGGAGAC 
CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCAACCA TTTCCACTGC AACCACAGCC 
CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CGTAAAACTA 
GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCGCGACTGA ACCCCATTCT ACCCCCTAAA 
GCCCCTGCGG TCTCTCCTCT CATAGGAAAT GGCACACAGT CCAACAACAC CTGCAGCTTG 
GGTGGGCCCA TGACGCTTCT CCCGGGCAGA AGCGACCTCT CTGGGGTGGA CGTCACTATG 
CTGAACATGC TCAACAGAAG GGACAGCAGC GCCAGCACCA TCAGCTCGGC CTACCTGAGC 
AGCCGCCGCT CCTCAGGGAT CTCGCCCTGC TTCTCCAGCC GCCGCTCCAG CGAGGCGTCA 
CAGGCCGAGG GCCGGCCGCA GAACGTGAGC GTGGCCGACT CCTACGACCC CATCTCCACC 
GACGCCTCGC GCCGCTCCAG CGAAGCCAGC CAGAGCGACG GCCTGCCCAG CCTGCTCAGC 
CTCACGCCCG CCCAGCAGTA CCGCCTCAAG GCCAAGTACG CGGCTGCCAC AGGAGGGCCG 
CCGCCCACGC CCCTGCCCAA CATGGAGAGG ATGAGCCTGA AGACGCGCCT GGCGCTGCTC 
GGGGATGCCC TCGAGCCTGG CGTGGCCCTG CCTCCAGTTC ATGCCCCGAG GAGGTGCAGC 
GACGGGGGAG CCCACGGCTA CGGGCGGCGC CACCTGCAGC CGCACGATGC GCCGGGCCAC 
GGCGTGAGGA GGGCCAGCGA CCCCGTGCGG ACAGGCTCCG AGGGCCTGGC CCTGCCTCGT 
GTGCCGCGCT TCAGCAGCCT CAGCAGCTGC AACCCCCCGG CGATGGCCAC GTCCGCGGAG 
AAGCGCAGTC TCGTGCTTCA GAATTACACG CGGCCCGAGG GCGGCCAGTC CCGAAACTTC 
CACTCGTCCC CCTGTCCTCC CAGCATCACC GAGAACGTCA CCCTGGAGTC CCTGACCATG 
GACGCTGATG CCAACCTGAA CGATGAGGAT TTCCTGCCGG ACGACGTGGT GCAGTATTTA 
AATTCCCAGA ACCAAGCAGG GTACGAGCAG CACTTCCCCA GCACCCTCCC GGACGACAGC 
AAAGTGCCCC ACGGGCCCGG TGACTTTGAC GCGCCCGGGC TGCCAGACAG CCACGCTGGC 
CAGCAGTTCC ATGCCCTCGA GCAGCCCTGC CCCGAGGGCA GCAAAACCGA CCTGCCCATT 
CAGTGGAACG AAGTCAGCTC CGGAAGCGCC GACCTGTCCT CCTCCAAGCT CAAGTGTGGG 
CCGCGGCCCG CTGTGCCGCA GACTCGCGCC TTTGGGTTCT GCAACGGCAT GGTCGTCCAC 
CCGCAGAACC CCTTGAGGAG CGGGCCTGCT GGGGGCTATC AGACCCTCGG GGAGAACAGC 
AACCCCTACG GTGGCCCAGA GCACTTGATG CTCCACAACA GCCCCGGAAG TGGCACCAGT 
GGAAACGCCT TCCATGAACA GCCCTGTAAG GCCCCGCAGT ATGGGAACTG TCTCAACAGG 
CAGCCAGTGG CCCCTGGTGC ACTCGACGGT GCCTGTGGTG CCGGGATTCA AGCCTCAAAG 
CTGAAGAGCA CCCCCATGCA AGGGAGCGGG GGCCAGCTGA ATTTCGGCCT GCCGGTAGCG 
CCAAATGAGT CAGCTGGCAG CATGGTGAAT GGCATGCAGA ACCAGGACCC AGTGGGACAG 
GGGTACCTGG CTCACCAGCT CCTCGGCGAC AGCATGCAGC ACCCGGGGGC AGGCCGCCCC 
GGTCAGCAGA TGCTTGGGCA GATTAGTGCT ACCTCACACA TCAACATCTA TCAAGGGCCA 
GAGAGCTGCC TGCCAGGGGC TCACGGCATG GGCAGCCAGC CGTCAAGCTT GGCAGTTGTC 
AGGGGCTACC AGCCATGTGC CAGCTTTGGG GGCAGCAGGC GCCAGGCTAT GCCGAGGGAC 
AGCCTTGCTC TGCAGTCAGG ACAGCTCAGT GACACAAGTC AGACCTGCAG GGTGAATGGC 
ATCAAGATGG AGATGAAAGG GCAGCCCCAT CCGCTGTGCT CTAATCTGCA GAATTACTCT 
GGTCAGTTCT ATGACCAAAC CGTGGGCTTC AGTCAGCAAG ACACGAAAGC TGGTTCATTC 
TCTATTTCAG ACGCCAGCTG CCTGCTACAG GGGACCAGCG CCAAAAACTC TGAGTTACTT 
TCCCCAGGTG CTAATCAGGT GACAAGCACA GTGGACAGCC TCGACAGCCA TGACCTGGAA 
GGGGTACAGA TTGACTTCGA TGCCATCATA GACGATGGGG ACCACTCCAG CCTGATGTCG 
GGGGCCCTGA GCCCAAGTAT CATTCAGAAC CTTTCCTATA GCTCCTCCCG CCTCACCACG 
CCTCGGGCGT CCCTCCCATT CCCAGCGCTG TCCATGAGCA CCACCAACAT GGCTATCGGG 
GACATGAGTT CTTTGCTGAC CTCCCTAGCG GAAGAAAGCA AATTCCTTGC AGTTATGCAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI3 ( XM_009452941.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009451216.1 Pan troglodytes GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.

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CloneID	OPc40512
Clone ID Related Accession (Same CDS sequence)	XM_009452941.2
Accession Version	XM_009452941.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	transcriptional activator GLI3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006474.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009452941.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCC AGTCCCACAG CTCCACGACC ACTGAAAAGA AAAAAGTTGA GAATTCCATA 
GTGAAGTGCT CCACTCGAAC AGATGTGAGC GAGAAAGCCG TTGCCTCCAG CACCACTTCT 
AATGAGGATG AAAGTCCTGG ACAGACTTAT CACAGAGAGA GAAGAAACGC AATCACTATG 
CAGCCACAGA ATGTCCAGGG GCTCAGCAAA GTCAGTGAGG AACCTTCAAC ATCGAGTGAC 
GAGAGGGCCT CATTGATCAA GAAAGAGATC CATGGGTCCC TGCCACACGT GGCAGAGCCC 
TCTGTGCCGT ACCGCGGGAC GGTGTTTGCC ATGGACCCCA GGAATGGTTA CATGGAGCCC 
CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA 
CATCATGAGG GCCGTTACCA TTACGATCCG TCTCCGATTC CTCCATTGCA TATGACTTCC 
GCCTTATCTA GTAGCCCTAC GTATCCGGAC CTGCCCTTCA TTAGGATCTC CCCACACCGG 
AACCCCGCTG CTGCTTCCGA GTCTCCCTTC AGCCCTCCAC ATCCCTACAT TAATCCCTAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCGCTCT CCATGATCTC AGCAACCCGT 
GGGCTGAGCC CTACAGATGC GCCCCATGCA GGAGTCAGCC CAGCAGAATA CTATCATCAG 
ATGGCCCTGC TAACTGGCCA GCGCAGCCCC TATGCAGACA TTATTCCCTC GGCTGCCACC 
GCCGGCACGG GGGCCATCCA CATGGAATAT CTTCATGCTA TGGATAGCAC CAGATTCCCC 
AGCCCCAGGC TGTCAGCCAG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTGTCC 
GATCATAGCT TTGACCTTCA GACCATGATA AGGACGTCTC CCAACTCCTT GGTCACGATT 
CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGCTCCT ATGGTCACTT ATCTGCAAGT 
GCAATCAGCC CTGCCTTGAG CTTCACCTAC TCTTCCGCGC CCGTCTCTCT CCACATGCAT 
CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGTTCAGCCT TTGGACACAG CCCTCCACTC 
ATCCACCCTG CCCCAACTTT TCCAACACAG AGGCCTATTC CAGGGATCCC TACGGTTCTG 
AACCCCGTCC AGGTCAGCTC CGGCCCTTCT GAGTCCTCAC AGAACAAGCC CACGAGTGAG 
TCTGCAGTGA GTAGCACTGG TGACCCGATG CACAACAAGA GGTCCAAGAT CAAACCCGAT 
GAAGACCTCC CCAGCCCAGG GGCTCGGGGG CAGCAGGAAC AGCCCGAAGG AACAACCCTT 
GTCAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CTGAAGTCAT CTATGAGACA 
AACTGCCACT GGGAAGGCTG CGCGAGGGAG TTCGACACCC AAGAGCAGCT TGTGCACCAT 
ATAAATAATG ACCATATTCA TGGAGAGAAG AAGGAGTTCG TGTGCAGGTG GCTGGACTGC 
TCAAGAGAGC AGAAACCCTT CAAAGCCCAG TATATGTTGG TAGTGCATAT GAGAAGACAC 
ACGGGCGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTA 
GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC 
GAAGGTTGCA ACAAGGCTTT CTCAAACGCC TCTGATCGCG CCAAACACCA AAACAGAACG 
CATTCCAATG AGAAACCATA TGTGTGCAAA ATCCCAGGCT GCACTAAGCG TTACACAGAC 
CCAAGCTCCC TCCGGAAACA TGTGAAGACA GTGCATGGCC CAGAGGCTCA TGTCACCAAG 
AAGCAGCGAG GGGACATCCA TCCTCGGCCA CCACCCCCGA GAGATTCCGG CAGCCATTCA 
CAGTCCAGGT CGCCCGGCCG ACCGACTCAG GGAGCCCTTG GTGAGCAGCA GGACCTCAGC 
AACACTACCT CAAAGCGGGA AGAATGCCTC CAGGTGAAAA CCGTCAAGGC AGAGAAGCCA 
ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC 
AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTAG TATAGGAGAC 
CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCAACCA TTTCCACTGC AACCACAGCC 
CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CGTAAAACTA 
GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCGCGACTGA ACCCCATTCT ACCCCCTAAA 
GCCCCTGCGG TCTCTCCTCT CATAGGAAAT GGCACACAGT CCAACAACAC CTGCAGCTTG 
GGTGGGCCCA TGACGCTTCT CCCGGGCAGA AGCGACCTCT CTGGGGTGGA CGTCACTATG 
CTGAACATGC TCAACAGAAG GGACAGCAGC GCCAGCACCA TCAGCTCGGC CTACCTGAGC 
AGCCGCCGCT CCTCAGGGAT CTCGCCCTGC TTCTCCAGCC GCCGCTCCAG CGAGGCGTCA 
CAGGCCGAGG GCCGGCCGCA GAACGTGAGC GTGGCCGACT CCTACGACCC CATCTCCACC 
GACGCCTCGC GCCGCTCCAG CGAAGCCAGC CAGAGCGACG GCCTGCCCAG CCTGCTCAGC 
CTCACGCCCG CCCAGCAGTA CCGCCTCAAG GCCAAGTACG CGGCTGCCAC AGGAGGGCCG 
CCGCCCACGC CCCTGCCCAA CATGGAGAGG ATGAGCCTGA AGACGCGCCT GGCGCTGCTC 
GGGGATGCCC TCGAGCCTGG CGTGGCCCTG CCTCCAGTTC ATGCCCCGAG GAGGTGCAGC 
GACGGGGGAG CCCACGGCTA CGGGCGGCGC CACCTGCAGC CGCACGATGC GCCGGGCCAC 
GGCGTGAGGA GGGCCAGCGA CCCCGTGCGG ACAGGCTCCG AGGGCCTGGC CCTGCCTCGT 
GTGCCGCGCT TCAGCAGCCT CAGCAGCTGC AACCCCCCGG CGATGGCCAC GTCCGCGGAG 
AAGCGCAGTC TCGTGCTTCA GAATTACACG CGGCCCGAGG GCGGCCAGTC CCGAAACTTC 
CACTCGTCCC CCTGTCCTCC CAGCATCACC GAGAACGTCA CCCTGGAGTC CCTGACCATG 
GACGCTGATG CCAACCTGAA CGATGAGGAT TTCCTGCCGG ACGACGTGGT GCAGTATTTA 
AATTCCCAGA ACCAAGCAGG GTACGAGCAG CACTTCCCCA GCGCCCTCCC GGACGACAGC 
AAAGTGCCCC ACGGGCCCGG TGACTTTGAC GCGCCCGGGC TGCCAGACAG CCACGCTGGC 
CAGCAGTTCC ATGCCCTCGA GCAGCCCTGC CCCGAGGGCA GCAAAACCGA CCTGCCCATT 
CAGTGGAACG AAGTCAGCTC CGGAAGCGCC GACCTGTCCT CCTCCAAGCT CAAGTGTGGG 
CCGCGGCCCG CTGTGCCGCA GACTCGCGCC TTTGGGTTCT GCAACGGCAT GGTCGTCCAC 
CCGCAGAACC CCTTGAGGAG CGGGCCTGCT GGGGGCTATC AGACCCTCGG GGAGAACAGC 
AACCCCTACG GTGGCCCAGA GCACTTGATG CTCCACAACA GCCCCGGAAG TGGCACCAGT 
GGAAACGCCT TCCATGAACA GCCCTGTAAG GCCCCGCAGT ATGGGAACTG TCTCAACAGG 
CAGCCAGTGG CCCCTGGTGC ACTCGACGGT GCCTGTGGTG CCGGGATTCA AGCCTCAAAG 
CTGAAGAGCA CCCCCATGCA AGGGAGCGGG GGCCAGCTGA ATTTCGGCCT GCCGGTAGCG 
CCAAATGAGT CAGCTGGCAG CATGGTGAAT GGCATGCAGA ACCAGGACCC AGTGGGACAG 
GGGTACCTGG CTCACCAGCT CCTCGGCGAC AGCATGCAGC ACCCGGGGGC AGGCCGCCCC 
GGTCAGCAGA TGCTTGGGCA GATTAGTGCT ACCTCACACA TCAACATCTA TCAAGGGCCA 
GAGAGCTGCC TGCCAGGGGC TCACGGCATG GGCAGCCAGC CGTCAAGCTT GGCAGTTGTC 
AGGGGCTACC AGCCATGTGC CAGCTTTGGG GGCAGCAGGC GCCAGGCTAT GCCGAGGGAC 
AGCCTTGCTC TGCAGTCAGG ACAGCTCAGT GACACAAGTC AGACCTGCAG GGTGAATGGC 
ATCAAGATGG AGATGAAAGG GCAGCCCCAT CCGCTGTGCT CTAATCTGCA GAATTACTCT 
GGTCAGTTCT ATGACCAAAC CGTGGGCTTC AGTCAGCAAG ACACGAAAGC TGGTTCATTC 
TCTATTTCAG ACGCCAGCTG CCTGCTACAG GGGACCAGCG CCAAAAACTC TGAGTTACTT 
TCCCCAGGTG CTAATCAGGT GACAAGCACA GTGGACAGCC TCGACAGCCA TGACCTGGAA 
GGGGTACAGA TTGACTTCGA TGCCATCATA GACGATGGGG ACCACTCCAG CCTGATGTCG 
GGGGCCCTGA GCCCAAGTAT CATTCAGAAC CTTTCCTATA GCTCCTCCCG CCTCACCACG 
CCTCGGGCGT CCCTCCCATT CCCAGCGCTG TCCATGAGCA CCACCAACAT GGCTATCGGG 
GACATGAGTT CTTTGCTGAC CTCCCTAGCG GAAGAAAGCA AATTCCTTGC AGTTATGCAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009451216.1
CDS	202..4944
Translation	MEAQSHSSTTTEKKKVENSIVKCSTRTDVSEKAVASSTTSNEDESPGQTYHRERRNAITMQPQNVQGLSKVSEEPSTSSDERASLIKKEIHGSLPHVAEPSVPYRGTVFAMDPRNGYMEPHYHPPHLFPAFHPPVPIDARHHEGRYHYDPSPIPPLHMTSALSSSPTYPDLPFIRISPHRNPAAASESPFSPPHPYINPYMDYIRSLHSSPSLSMISATRGLSPTDAPHAGVSPAEYYHQMALLTGQRSPYADIIPSAATAGTGAIHMEYLHAMDSTRFPSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYSSAPVSLHMHQQILSRQQSLGSAFGHSPPLIHPAPTFPTQRPIPGIPTVLNPVQVSSGPSESSQNKPTSESAVSSTGDPMHNKRSKIKPDEDLPSPGARGQQEQPEGTTLVKEEGDKDESKQEPEVIYETNCHWEGCAREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPRDSGSHSQSRSPGRPTQGALGEQQDLSNTTSKREECLQVKTVKAEKPMTSQPSPGGQSSCSSQQSPISNYSNSGLELPLTDGGSIGDLSAIDETPIMDSTISTATTALALQARRNPAGTKWMEHVKLERLKQVNGMFPRLNPILPPKAPAVSPLIGNGTQSNNTCSLGGPMTLLPGRSDLSGVDVTMLNMLNRRDSSASTISSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSEASQSDGLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRLALLGDALEPGVALPPVHAPRRCSDGGAHGYGRRHLQPHDAPGHGVRRASDPVRTGSEGLALPRVPRFSSLSSCNPPAMATSAEKRSLVLQNYTRPEGGQSRNFHSSPCPPSITENVTLESLTMDADANLNDEDFLPDDVVQYLNSQNQAGYEQHFPSALPDDSKVPHGPGDFDAPGLPDSHAGQQFHALEQPCPEGSKTDLPIQWNEVSSGSADLSSSKLKCGPRPAVPQTRAFGFCNGMVVHPQNPLRSGPAGGYQTLGENSNPYGGPEHLMLHNSPGSGTSGNAFHEQPCKAPQYGNCLNRQPVAPGALDGACGAGIQASKLKSTPMQGSGGQLNFGLPVAPNESAGSMVNGMQNQDPVGQGYLAHQLLGDSMQHPGAGRPGQQMLGQISATSHINIYQGPESCLPGAHGMGSQPSSLAVVRGYQPCASFGGSRRQAMPRDSLALQSGQLSDTSQTCRVNGIKMEMKGQPHPLCSNLQNYSGQFYDQTVGFSQQDTKAGSFSISDASCLLQGTSAKNSELLSPGANQVTSTVDSLDSHDLEGVQIDFDAIIDDGDHSSLMSGALSPSIIQNLSYSSSRLTTPRASLPFPALSMSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	XM_009452941.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009452941.2

ORF Insert Sequence:

ATGGAGGCCC AGTCCCACAG CTCCACGACC ACTGAAAAGA AAAAAGTTGA GAATTCCATA 
GTGAAGTGCT CCACTCGAAC AGATGTGAGC GAGAAAGCCG TTGCCTCCAG CACCACTTCT 
AATGAGGATG AAAGTCCTGG ACAGACTTAT CACAGAGAGA GAAGAAACGC AATCACTATG 
CAGCCACAGA ATGTCCAGGG GCTCAGCAAA GTCAGTGAGG AACCTTCAAC ATCGAGTGAC 
GAGAGGGCCT CATTGATCAA GAAAGAGATC CATGGGTCCC TGCCACACGT GGCAGAGCCC 
TCTGTGCCGT ACCGCGGGAC GGTGTTTGCC ATGGACCCCA GGAATGGTTA CATGGAGCCC 
CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA 
CATCATGAGG GCCGTTACCA TTACGATCCG TCTCCGATTC CTCCATTGCA TATGACTTCC 
GCCTTATCTA GTAGCCCTAC GTATCCGGAC CTGCCCTTCA TTAGGATCTC CCCACACCGG 
AACCCCGCTG CTGCTTCCGA GTCTCCCTTC AGCCCTCCAC ATCCCTACAT TAATCCCTAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCGCTCT CCATGATCTC AGCAACCCGT 
GGGCTGAGCC CTACAGATGC GCCCCATGCA GGAGTCAGCC CAGCAGAATA CTATCATCAG 
ATGGCCCTGC TAACTGGCCA GCGCAGCCCC TATGCAGACA TTATTCCCTC GGCTGCCACC 
GCCGGCACGG GGGCCATCCA CATGGAATAT CTTCATGCTA TGGATAGCAC CAGATTCCCC 
AGCCCCAGGC TGTCAGCCAG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTGTCC 
GATCATAGCT TTGACCTTCA GACCATGATA AGGACGTCTC CCAACTCCTT GGTCACGATT 
CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGCTCCT ATGGTCACTT ATCTGCAAGT 
GCAATCAGCC CTGCCTTGAG CTTCACCTAC TCTTCCGCGC CCGTCTCTCT CCACATGCAT 
CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGTTCAGCCT TTGGACACAG CCCTCCACTC 
ATCCACCCTG CCCCAACTTT TCCAACACAG AGGCCTATTC CAGGGATCCC TACGGTTCTG 
AACCCCGTCC AGGTCAGCTC CGGCCCTTCT GAGTCCTCAC AGAACAAGCC CACGAGTGAG 
TCTGCAGTGA GTAGCACTGG TGACCCGATG CACAACAAGA GGTCCAAGAT CAAACCCGAT 
GAAGACCTCC CCAGCCCAGG GGCTCGGGGG CAGCAGGAAC AGCCCGAAGG AACAACCCTT 
GTCAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CTGAAGTCAT CTATGAGACA 
AACTGCCACT GGGAAGGCTG CGCGAGGGAG TTCGACACCC AAGAGCAGCT TGTGCACCAT 
ATAAATAATG ACCATATTCA TGGAGAGAAG AAGGAGTTCG TGTGCAGGTG GCTGGACTGC 
TCAAGAGAGC AGAAACCCTT CAAAGCCCAG TATATGTTGG TAGTGCATAT GAGAAGACAC 
ACGGGCGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTA 
GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC 
GAAGGTTGCA ACAAGGCTTT CTCAAACGCC TCTGATCGCG CCAAACACCA AAACAGAACG 
CATTCCAATG AGAAACCATA TGTGTGCAAA ATCCCAGGCT GCACTAAGCG TTACACAGAC 
CCAAGCTCCC TCCGGAAACA TGTGAAGACA GTGCATGGCC CAGAGGCTCA TGTCACCAAG 
AAGCAGCGAG GGGACATCCA TCCTCGGCCA CCACCCCCGA GAGATTCCGG CAGCCATTCA 
CAGTCCAGGT CGCCCGGCCG ACCGACTCAG GGAGCCCTTG GTGAGCAGCA GGACCTCAGC 
AACACTACCT CAAAGCGGGA AGAATGCCTC CAGGTGAAAA CCGTCAAGGC AGAGAAGCCA 
ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC 
AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTAG TATAGGAGAC 
CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCAACCA TTTCCACTGC AACCACAGCC 
CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CGTAAAACTA 
GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCGCGACTGA ACCCCATTCT ACCCCCTAAA 
GCCCCTGCGG TCTCTCCTCT CATAGGAAAT GGCACACAGT CCAACAACAC CTGCAGCTTG 
GGTGGGCCCA TGACGCTTCT CCCGGGCAGA AGCGACCTCT CTGGGGTGGA CGTCACTATG 
CTGAACATGC TCAACAGAAG GGACAGCAGC GCCAGCACCA TCAGCTCGGC CTACCTGAGC 
AGCCGCCGCT CCTCAGGGAT CTCGCCCTGC TTCTCCAGCC GCCGCTCCAG CGAGGCGTCA 
CAGGCCGAGG GCCGGCCGCA GAACGTGAGC GTGGCCGACT CCTACGACCC CATCTCCACC 
GACGCCTCGC GCCGCTCCAG CGAAGCCAGC CAGAGCGACG GCCTGCCCAG CCTGCTCAGC 
CTCACGCCCG CCCAGCAGTA CCGCCTCAAG GCCAAGTACG CGGCTGCCAC AGGAGGGCCG 
CCGCCCACGC CCCTGCCCAA CATGGAGAGG ATGAGCCTGA AGACGCGCCT GGCGCTGCTC 
GGGGATGCCC TCGAGCCTGG CGTGGCCCTG CCTCCAGTTC ATGCCCCGAG GAGGTGCAGC 
GACGGGGGAG CCCACGGCTA CGGGCGGCGC CACCTGCAGC CGCACGATGC GCCGGGCCAC 
GGCGTGAGGA GGGCCAGCGA CCCCGTGCGG ACAGGCTCCG AGGGCCTGGC CCTGCCTCGT 
GTGCCGCGCT TCAGCAGCCT CAGCAGCTGC AACCCCCCGG CGATGGCCAC GTCCGCGGAG 
AAGCGCAGTC TCGTGCTTCA GAATTACACG CGGCCCGAGG GCGGCCAGTC CCGAAACTTC 
CACTCGTCCC CCTGTCCTCC CAGCATCACC GAGAACGTCA CCCTGGAGTC CCTGACCATG 
GACGCTGATG CCAACCTGAA CGATGAGGAT TTCCTGCCGG ACGACGTGGT GCAGTATTTA 
AATTCCCAGA ACCAAGCAGG GTACGAGCAG CACTTCCCCA GCGCCCTCCC GGACGACAGC 
AAAGTGCCCC ACGGGCCCGG TGACTTTGAC GCGCCCGGGC TGCCAGACAG CCACGCTGGC 
CAGCAGTTCC ATGCCCTCGA GCAGCCCTGC CCCGAGGGCA GCAAAACCGA CCTGCCCATT 
CAGTGGAACG AAGTCAGCTC CGGAAGCGCC GACCTGTCCT CCTCCAAGCT CAAGTGTGGG 
CCGCGGCCCG CTGTGCCGCA GACTCGCGCC TTTGGGTTCT GCAACGGCAT GGTCGTCCAC 
CCGCAGAACC CCTTGAGGAG CGGGCCTGCT GGGGGCTATC AGACCCTCGG GGAGAACAGC 
AACCCCTACG GTGGCCCAGA GCACTTGATG CTCCACAACA GCCCCGGAAG TGGCACCAGT 
GGAAACGCCT TCCATGAACA GCCCTGTAAG GCCCCGCAGT ATGGGAACTG TCTCAACAGG 
CAGCCAGTGG CCCCTGGTGC ACTCGACGGT GCCTGTGGTG CCGGGATTCA AGCCTCAAAG 
CTGAAGAGCA CCCCCATGCA AGGGAGCGGG GGCCAGCTGA ATTTCGGCCT GCCGGTAGCG 
CCAAATGAGT CAGCTGGCAG CATGGTGAAT GGCATGCAGA ACCAGGACCC AGTGGGACAG 
GGGTACCTGG CTCACCAGCT CCTCGGCGAC AGCATGCAGC ACCCGGGGGC AGGCCGCCCC 
GGTCAGCAGA TGCTTGGGCA GATTAGTGCT ACCTCACACA TCAACATCTA TCAAGGGCCA 
GAGAGCTGCC TGCCAGGGGC TCACGGCATG GGCAGCCAGC CGTCAAGCTT GGCAGTTGTC 
AGGGGCTACC AGCCATGTGC CAGCTTTGGG GGCAGCAGGC GCCAGGCTAT GCCGAGGGAC 
AGCCTTGCTC TGCAGTCAGG ACAGCTCAGT GACACAAGTC AGACCTGCAG GGTGAATGGC 
ATCAAGATGG AGATGAAAGG GCAGCCCCAT CCGCTGTGCT CTAATCTGCA GAATTACTCT 
GGTCAGTTCT ATGACCAAAC CGTGGGCTTC AGTCAGCAAG ACACGAAAGC TGGTTCATTC 
TCTATTTCAG ACGCCAGCTG CCTGCTACAG GGGACCAGCG CCAAAAACTC TGAGTTACTT 
TCCCCAGGTG CTAATCAGGT GACAAGCACA GTGGACAGCC TCGACAGCCA TGACCTGGAA 
GGGGTACAGA TTGACTTCGA TGCCATCATA GACGATGGGG ACCACTCCAG CCTGATGTCG 
GGGGCCCTGA GCCCAAGTAT CATTCAGAAC CTTTCCTATA GCTCCTCCCG CCTCACCACG 
CCTCGGGCGT CCCTCCCATT CCCAGCGCTG TCCATGAGCA CCACCAACAT GGCTATCGGG 
GACATGAGTT CTTTGCTGAC CTCCCTAGCG GAAGAAAGCA AATTCCTTGC AGTTATGCAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Accelerated evolution of nervous system genes in the origin of Homo sapiens.
Cell119(7)1027-40(2004 Dec)
Dorus S,Vallender EJ,Evans PD,Anderson JR,Gilbert SL,Mahowald M,Wyckoff GJ,Malcom CM,Lahn BT

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GLI3 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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