RASGRF1 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene
Clones
gRNAs

Summary

Gene Symbol	RASGRF1
Entrez Gene ID	453575
Full Name	Ras protein specific guanine nucleotide releasing factor 1
General protein information	Preferred Names Ras protein specific guanine nucleotide releasing factor 1 Names ras-specific guanine nucleotide-releasing factor 1
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001153395.4	XP_001153395.2	ras-specific guanine nucleotide-releasing factor 1 isoform X4
XM_009429654.2	XP_009427929.1	ras-specific guanine nucleotide-releasing factor 1 isoform X2
XM_001153586.4	XP_001153586.1	ras-specific guanine nucleotide-releasing factor 1 isoform X1
XM_009429655.2	XP_009427930.1	ras-specific guanine nucleotide-releasing factor 1 isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	RASGRF1	NP_002882.3
Mus musculus (house mouse)	Rasgrf1	NP_035375.1
Danio rerio (zebrafish)	si:ch211-234p18.3	XP_002665395.1
Xenopus tropicalis (tropical clawed frog)	rasgrf1	XP_002933009.2
Gallus gallus (chicken)	RASGRF1	XP_004944008.1
Pan troglodytes (chimpanzee)	RASGRF1	XP_001153586.1
Canis lupus familiaris (dog)	RASGRF1	XP_545892.5
Rattus norvegicus (Norway rat)	Rasgrf1	NP_001164002.1
Bos taurus (cattle)	RASGRF1	NP_001178386.1

Gene Ontology

The following RASGRF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASGRF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc68941	XM_001153586.4 Latest version!	Pan troglodytes Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OPc68942	XM_009429654.2 Latest version!	Pan troglodytes Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OPc68943	XM_009429655.2 Latest version!	Pan troglodytes Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OPc68944	XM_001153395.4 Latest version!	Pan troglodytes Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RASGRF1 ( XM_001153586.4 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_001153586.1 Pan troglodytes Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPc68941
Clone ID Related Accession (Same CDS sequence)	XM_001153586.4
Accession Version	XM_001153586.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_001153586.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC 
CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG 
GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG 
CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACGGAGA AGACCGTGGC CAAGCAGCTT 
CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCGCATCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC 
AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC 
ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCTG CCAGCTCCAA GAAGCCTCCC 
ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTGCAT 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT 
GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT 
CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA 
GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT 
ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC 
CAAGACACAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT 
ACGGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC 
AAGGTCACTG TGCCGCAGAT GATCAAGAGG ACCAGGGAGG GGACCAGGGA AGCAGAAATG 
AGCAGGTCCG ACGCCTCCTT ATATTGTGAT GATGTTGACA TTCGCTTCAG CAAAACCATG 
AACTCCTGCA AAGTGCTGCA GATCCGCTAC GCCAGTGTGG AGCGGCTGCT GGAGAGGCTG 
ACGGACCTGC GCTTCCTGAG CATCGACTTC CTCAACACCT TCCTGCACTC CTACCGCGTC 
TTCACCACCG CCATCGTGGT CCTGGACAAG CTCATTACCA TCTACAAGAA GCCTATCAGT 
GCCATTCCTG CCAGGTGGCT GAGGTCGCTG GAGCTCCTGT TTGCCAGTGG CCAGAACAAT 
AAGCTCCTGT ACGGTGAACC CCCCAAGTCC CCGCGCGCCA CCCGCAAGTT CTCCTCGCCG 
CCACCTCTGT CCATCACCAA GACATCGTCA CCGAGCCGCC GGCGGAAGCT CTCCCTGAAC 
ATCCCCATCA TCACCGGCGG CAAGGCCCTG GACCTGGCCG CCCTCAGCTG CAACTCCAAT 
GGCTACACCA GCATGTACTC GGCCATGTCG CCCTTCAGCA AGGCCACGCT GGACACCAGC 
AAGCTCTATG TGTCCAGCAG CTTCACCAAC AAGATTCCAG ATGAGGGCGA TACGACCCCT 
GAGAAGCCCG AAGACCCTTC GGCGCTCAGC AAGCAGAGCT CAGAAGTCTC CATGAGAGAG 
GAGTCAGATA TTGATCAAAA CCAGAGTGAT GATGGTGATA CTGAAACATC ACCAACTAAA 
TCTCCAACAA CACCCAAATC AGTCAAAAGC AAAAATTCTT CAGAGTTCCC GCTCTTTTCC 
TATAACAATG GAGTCGTCAT GACCTCCTGT CGTGAACTGG ACAATAACCG CAGTGCCTTG 
TCGGCCGCCT CTGCCTTTGC CATAGCAACC GCGGGGGCCA ACGAGGGCAC CCCAAACAAG 
GAGAAGTACC GGAGGATGTC CTTAGCCAGT GCAGGGTTTC CCCCAGACCA GAGGAATGGA 
GACAAGGAGT TTGTGATCCG CAGAGCAGCC ACCAATCGTG TCTTGAACGT GCTCCGCCAC 
TGGGTGTCCA AGCACTCTCA GGACTTTGAG ACCAACGATG AGCTCAAATG CAAGGTGATC 
GGCTTCCTGG AAGAAGTCAT GCACGACCCG GAGCTCCTGA CCCAGGAGCG GAAGGCTGCA 
GCCAACATCA TCAGGACTCT GACCCAGGAG GACCCAGGTG ACAACCAGAT CACGCTGGAG 
GAGATCACGC AGATGGCTGA AGGCGTGAAG GCTGAGCCCT TTGAAAACCA CTCAGCCCTG 
GAGATCGCGG AGCAGCTGAC CCTGCTAGAT CACCTCGTCT TCAAGAAGAT TCCTTATGAG 
GAGTTCTTCG GACAAGGATG GATGAAACTG GAAAAGAATG AAAGGACCCC TTATATCATG 
AAAACCACTA AGCACTTCAA TGACATCAGT AACTTGATTG CTTCAGAAAT CATCCGCAAC 
GAGGACATCA ACGCCAGGGT GAGCGCCATC GAGAAGTGGG TGGCCGTAGC TGACATATGC 
CGCTGCCTCC ACAACTACAA TGCCGTACTG GAGATCACCT CGTCCATGAA CCGCAGTGCA 
ATCTTCCGGC TCAAAAAGAC GTGGCTCAAA GTCTCTAAGC AGACTAAAGC TTTGATTGAT 
AAGCTCCAAA AGCTTGTGTC ATCTGAGGGC AGATTTAAGA ATCTCAGAGA AGCTCTGAAA 
AATTGTGACC CACCCTGTGT CCCTTACCTG GGGATGTACC TCACCGACCT GGCCTTCATC 
GAGGAGGGGA CGCCCAATTA CACGGAAGAC GGCCTGGTCA ACTTCTCCAA GATGAGGATG 
ATATCCCATA TTATCCGAGA GATTCGCCAG TTTCAACAAA CTGCCTACAA AATAGAGCAC 
CAAGCAAAGG TAACGCAATA TTTACTGGAC CAATCTTTTG TAATGGATGA AGAAAGCCTC 
TACGAGTCTT CTCTCCGAAT AGAACCAAAA CTCCCCACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001153586.1
CDS	440..4261
Translation	MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEGCVCDRAPSPKPALSAKEPLEKQHYFTVNFSHENQKALELRTEDAKDCDEWVAAIAHASYRTLATEHEALMQKYLHLLQIVETEKTVAKQLRQQIEDGEIEIERLKAEIASLLKDNERIQSTQTVAPNDEDSDIKKIKKVQSFLRGWLCRRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIIEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLEEPESTEEEAKGSGQDTDHLDFKIGVEPKDSPPFTVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKRTREGTREAEMSRSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAIVVLDKLITIYKKPISAIPARWLRSLELLFASGQNNKLLYGEPPKSPRATRKFSSPPPLSITKTSSPSRRRKLSLNIPIITGGKALDLAALSCNSNGYTSMYSAMSPFSKATLDTSKLYVSSSFTNKIPDEGDTTPEKPEDPSALSKQSSEVSMREESDIDQNQSDDGDTETSPTKSPTTPKSVKSKNSSEFPLFSYNNGVVMTSCRELDNNRSALSAASAFAIATAGANEGTPNKEKYRRMSLASAGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELKCKVIGFLEEVMHDPELLTQERKAAANIIRTLTQEDPGDNQITLEEITQMAEGVKAEPFENHSALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDISNLIASEIIRNEDINARVSAIEKWVAVADICRCLHNYNAVLEITSSMNRSAIFRLKKTWLKVSKQTKALIDKLQKLVSSEGRFKNLREALKNCDPPCVPYLGMYLTDLAFIEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTAYKIEHQAKVTQYLLDQSFVMDEESLYESSLRIEPKLPT

Target ORF information:

RefSeq Version	XM_001153586.4
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001153586.4

ORF Insert Sequence:

ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC 
CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG 
GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG 
CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACGGAGA AGACCGTGGC CAAGCAGCTT 
CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCGCATCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC 
AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC 
ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCTG CCAGCTCCAA GAAGCCTCCC 
ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTGCAT 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT 
GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT 
CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA 
GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT 
ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC 
CAAGACACAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT 
ACGGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC 
AAGGTCACTG TGCCGCAGAT GATCAAGAGG ACCAGGGAGG GGACCAGGGA AGCAGAAATG 
AGCAGGTCCG ACGCCTCCTT ATATTGTGAT GATGTTGACA TTCGCTTCAG CAAAACCATG 
AACTCCTGCA AAGTGCTGCA GATCCGCTAC GCCAGTGTGG AGCGGCTGCT GGAGAGGCTG 
ACGGACCTGC GCTTCCTGAG CATCGACTTC CTCAACACCT TCCTGCACTC CTACCGCGTC 
TTCACCACCG CCATCGTGGT CCTGGACAAG CTCATTACCA TCTACAAGAA GCCTATCAGT 
GCCATTCCTG CCAGGTGGCT GAGGTCGCTG GAGCTCCTGT TTGCCAGTGG CCAGAACAAT 
AAGCTCCTGT ACGGTGAACC CCCCAAGTCC CCGCGCGCCA CCCGCAAGTT CTCCTCGCCG 
CCACCTCTGT CCATCACCAA GACATCGTCA CCGAGCCGCC GGCGGAAGCT CTCCCTGAAC 
ATCCCCATCA TCACCGGCGG CAAGGCCCTG GACCTGGCCG CCCTCAGCTG CAACTCCAAT 
GGCTACACCA GCATGTACTC GGCCATGTCG CCCTTCAGCA AGGCCACGCT GGACACCAGC 
AAGCTCTATG TGTCCAGCAG CTTCACCAAC AAGATTCCAG ATGAGGGCGA TACGACCCCT 
GAGAAGCCCG AAGACCCTTC GGCGCTCAGC AAGCAGAGCT CAGAAGTCTC CATGAGAGAG 
GAGTCAGATA TTGATCAAAA CCAGAGTGAT GATGGTGATA CTGAAACATC ACCAACTAAA 
TCTCCAACAA CACCCAAATC AGTCAAAAGC AAAAATTCTT CAGAGTTCCC GCTCTTTTCC 
TATAACAATG GAGTCGTCAT GACCTCCTGT CGTGAACTGG ACAATAACCG CAGTGCCTTG 
TCGGCCGCCT CTGCCTTTGC CATAGCAACC GCGGGGGCCA ACGAGGGCAC CCCAAACAAG 
GAGAAGTACC GGAGGATGTC CTTAGCCAGT GCAGGGTTTC CCCCAGACCA GAGGAATGGA 
GACAAGGAGT TTGTGATCCG CAGAGCAGCC ACCAATCGTG TCTTGAACGT GCTCCGCCAC 
TGGGTGTCCA AGCACTCTCA GGACTTTGAG ACCAACGATG AGCTCAAATG CAAGGTGATC 
GGCTTCCTGG AAGAAGTCAT GCACGACCCG GAGCTCCTGA CCCAGGAGCG GAAGGCTGCA 
GCCAACATCA TCAGGACTCT GACCCAGGAG GACCCAGGTG ACAACCAGAT CACGCTGGAG 
GAGATCACGC AGATGGCTGA AGGCGTGAAG GCTGAGCCCT TTGAAAACCA CTCAGCCCTG 
GAGATCGCGG AGCAGCTGAC CCTGCTAGAT CACCTCGTCT TCAAGAAGAT TCCTTATGAG 
GAGTTCTTCG GACAAGGATG GATGAAACTG GAAAAGAATG AAAGGACCCC TTATATCATG 
AAAACCACTA AGCACTTCAA TGACATCAGT AACTTGATTG CTTCAGAAAT CATCCGCAAC 
GAGGACATCA ACGCCAGGGT GAGCGCCATC GAGAAGTGGG TGGCCGTAGC TGACATATGC 
CGCTGCCTCC ACAACTACAA TGCCGTACTG GAGATCACCT CGTCCATGAA CCGCAGTGCA 
ATCTTCCGGC TCAAAAAGAC GTGGCTCAAA GTCTCTAAGC AGACTAAAGC TTTGATTGAT 
AAGCTCCAAA AGCTTGTGTC ATCTGAGGGC AGATTTAAGA ATCTCAGAGA AGCTCTGAAA 
AATTGTGACC CACCCTGTGT CCCTTACCTG GGGATGTACC TCACCGACCT GGCCTTCATC 
GAGGAGGGGA CGCCCAATTA CACGGAAGAC GGCCTGGTCA ACTTCTCCAA GATGAGGATG 
ATATCCCATA TTATCCGAGA GATTCGCCAG TTTCAACAAA CTGCCTACAA AATAGAGCAC 
CAAGCAAAGG TAACGCAATA TTTACTGGAC CAATCTTTTG TAATGGATGA AGAAAGCCTC 
TACGAGTCTT CTCTCCGAAT AGAACCAAAA CTCCCCACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF1 ( XM_009429654.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009427929.1 Pan troglodytes Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPc68942
Clone ID Related Accession (Same CDS sequence)	XM_009429654.2
Accession Version	XM_009429654.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009429654.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC 
CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG 
GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG 
CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACGGAGA AGACCGTGGC CAAGCAGCTT 
CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCGCATCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC 
AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC 
ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCTG CCAGCTCCAA GAAGCCTCCC 
ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTGCAT 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT 
GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT 
CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA 
GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT 
ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC 
CAAGACACAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT 
ACGGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC 
AAGGTCACTG TGCCGCAGAT GATCAAGAGG ACCAGGGAGG GGACCAGGGA AGCAGAAATG 
AGCAGGTCCG ACGCCTCCTT ATATTGTGAT GATGTTGACA TTCGCTTCAG CAAAACCATG 
AACTCCTGCA AAGTGCTGCA GATCCGCTAC GCCAGTGTGG AGCGGCTGCT GGAGAGGCTG 
ACGGACCTGC GCTTCCTGAG CATCGACTTC CTCAACACCT TCCTGCACTC CTACCGCGTC 
TTCACCACCG CCATCGTGGT CCTGGACAAG CTCATTACCA TCTACAAGAA GCCTATCAGT 
GCCATTCCTG CCAGGTCGCT GGAGCTCCTG TTTGCCAGTG GCCAGAACAA TAAGCTCCTG 
TACGGTGAAC CCCCCAAGTC CCCGCGCGCC ACCCGCAAGT TCTCCTCGCC GCCACCTCTG 
TCCATCACCA AGACATCGTC ACCGAGCCGC CGGCGGAAGC TCTCCCTGAA CATCCCCATC 
ATCACCGGCG GCAAGGCCCT GGACCTGGCC GCCCTCAGCT GCAACTCCAA TGGCTACACC 
AGCATGTACT CGGCCATGTC GCCCTTCAGC AAGGCCACGC TGGACACCAG CAAGCTCTAT 
GTGTCCAGCA GCTTCACCAA CAAGATTCCA GATGAGGGCG ATACGACCCC TGAGAAGCCC 
GAAGACCCTT CGGCGCTCAG CAAGCAGAGC TCAGAAGTCT CCATGAGAGA GGAGTCAGAT 
ATTGATCAAA ACCAGAGTGA TGATGGTGAT ACTGAAACAT CACCAACTAA ATCTCCAACA 
ACACCCAAAT CAGTCAAAAG CAAAAATTCT TCAGAGTTCC CGCTCTTTTC CTATAACAAT 
GGAGTCGTCA TGACCTCCTG TCGTGAACTG GACAATAACC GCAGTGCCTT GTCGGCCGCC 
TCTGCCTTTG CCATAGCAAC CGCGGGGGCC AACGAGGGCA CCCCAAACAA GGAGAAGTAC 
CGGAGGATGT CCTTAGCCAG TGCAGGGTTT CCCCCAGACC AGAGGAATGG AGACAAGGAG 
TTTGTGATCC GCAGAGCAGC CACCAATCGT GTCTTGAACG TGCTCCGCCA CTGGGTGTCC 
AAGCACTCTC AGGACTTTGA GACCAACGAT GAGCTCAAAT GCAAGGTGAT CGGCTTCCTG 
GAAGAAGTCA TGCACGACCC GGAGCTCCTG ACCCAGGAGC GGAAGGCTGC AGCCAACATC 
ATCAGGACTC TGACCCAGGA GGACCCAGGT GACAACCAGA TCACGCTGGA GGAGATCACG 
CAGATGGCTG AAGGCGTGAA GGCTGAGCCC TTTGAAAACC ACTCAGCCCT GGAGATCGCG 
GAGCAGCTGA CCCTGCTAGA TCACCTCGTC TTCAAGAAGA TTCCTTATGA GGAGTTCTTC 
GGACAAGGAT GGATGAAACT GGAAAAGAAT GAAAGGACCC CTTATATCAT GAAAACCACT 
AAGCACTTCA ATGACATCAG TAACTTGATT GCTTCAGAAA TCATCCGCAA CGAGGACATC 
AACGCCAGGG TGAGCGCCAT CGAGAAGTGG GTGGCCGTAG CTGACATATG CCGCTGCCTC 
CACAACTACA ATGCCGTACT GGAGATCACC TCGTCCATGA ACCGCAGTGC AATCTTCCGG 
CTCAAAAAGA CGTGGCTCAA AGTCTCTAAG CAGACTAAAG CTTTGATTGA TAAGCTCCAA 
AAGCTTGTGT CATCTGAGGG CAGATTTAAG AATCTCAGAG AAGCTCTGAA AAATTGTGAC 
CCACCCTGTG TCCCTTACCT GGGGATGTAC CTCACCGACC TGGCCTTCAT CGAGGAGGGG 
ACGCCCAATT ACACGGAAGA CGGCCTGGTC AACTTCTCCA AGATGAGGAT GATATCCCAT 
ATTATCCGAG AGATTCGCCA GTTTCAACAA ACTGCCTACA AAATAGAGCA CCAAGCAAAG 
GTAACGCAAT ATTTACTGGA CCAATCTTTT GTAATGGATG AAGAAAGCCT CTACGAGTCT 
TCTCTCCGAA TAGAACCAAA ACTCCCCACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009427929.1
CDS	440..4252
Translation	MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEGCVCDRAPSPKPALSAKEPLEKQHYFTVNFSHENQKALELRTEDAKDCDEWVAAIAHASYRTLATEHEALMQKYLHLLQIVETEKTVAKQLRQQIEDGEIEIERLKAEIASLLKDNERIQSTQTVAPNDEDSDIKKIKKVQSFLRGWLCRRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIIEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLEEPESTEEEAKGSGQDTDHLDFKIGVEPKDSPPFTVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKRTREGTREAEMSRSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAIVVLDKLITIYKKPISAIPARSLELLFASGQNNKLLYGEPPKSPRATRKFSSPPPLSITKTSSPSRRRKLSLNIPIITGGKALDLAALSCNSNGYTSMYSAMSPFSKATLDTSKLYVSSSFTNKIPDEGDTTPEKPEDPSALSKQSSEVSMREESDIDQNQSDDGDTETSPTKSPTTPKSVKSKNSSEFPLFSYNNGVVMTSCRELDNNRSALSAASAFAIATAGANEGTPNKEKYRRMSLASAGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELKCKVIGFLEEVMHDPELLTQERKAAANIIRTLTQEDPGDNQITLEEITQMAEGVKAEPFENHSALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDISNLIASEIIRNEDINARVSAIEKWVAVADICRCLHNYNAVLEITSSMNRSAIFRLKKTWLKVSKQTKALIDKLQKLVSSEGRFKNLREALKNCDPPCVPYLGMYLTDLAFIEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTAYKIEHQAKVTQYLLDQSFVMDEESLYESSLRIEPKLPT

Target ORF information:

RefSeq Version	XM_009429654.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009429654.2

ORF Insert Sequence:

ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC 
CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG 
GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG 
CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACGGAGA AGACCGTGGC CAAGCAGCTT 
CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCGCATCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC 
AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC 
ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCTG CCAGCTCCAA GAAGCCTCCC 
ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTGCAT 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT 
GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT 
CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA 
GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT 
ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC 
CAAGACACAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT 
ACGGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC 
AAGGTCACTG TGCCGCAGAT GATCAAGAGG ACCAGGGAGG GGACCAGGGA AGCAGAAATG 
AGCAGGTCCG ACGCCTCCTT ATATTGTGAT GATGTTGACA TTCGCTTCAG CAAAACCATG 
AACTCCTGCA AAGTGCTGCA GATCCGCTAC GCCAGTGTGG AGCGGCTGCT GGAGAGGCTG 
ACGGACCTGC GCTTCCTGAG CATCGACTTC CTCAACACCT TCCTGCACTC CTACCGCGTC 
TTCACCACCG CCATCGTGGT CCTGGACAAG CTCATTACCA TCTACAAGAA GCCTATCAGT 
GCCATTCCTG CCAGGTCGCT GGAGCTCCTG TTTGCCAGTG GCCAGAACAA TAAGCTCCTG 
TACGGTGAAC CCCCCAAGTC CCCGCGCGCC ACCCGCAAGT TCTCCTCGCC GCCACCTCTG 
TCCATCACCA AGACATCGTC ACCGAGCCGC CGGCGGAAGC TCTCCCTGAA CATCCCCATC 
ATCACCGGCG GCAAGGCCCT GGACCTGGCC GCCCTCAGCT GCAACTCCAA TGGCTACACC 
AGCATGTACT CGGCCATGTC GCCCTTCAGC AAGGCCACGC TGGACACCAG CAAGCTCTAT 
GTGTCCAGCA GCTTCACCAA CAAGATTCCA GATGAGGGCG ATACGACCCC TGAGAAGCCC 
GAAGACCCTT CGGCGCTCAG CAAGCAGAGC TCAGAAGTCT CCATGAGAGA GGAGTCAGAT 
ATTGATCAAA ACCAGAGTGA TGATGGTGAT ACTGAAACAT CACCAACTAA ATCTCCAACA 
ACACCCAAAT CAGTCAAAAG CAAAAATTCT TCAGAGTTCC CGCTCTTTTC CTATAACAAT 
GGAGTCGTCA TGACCTCCTG TCGTGAACTG GACAATAACC GCAGTGCCTT GTCGGCCGCC 
TCTGCCTTTG CCATAGCAAC CGCGGGGGCC AACGAGGGCA CCCCAAACAA GGAGAAGTAC 
CGGAGGATGT CCTTAGCCAG TGCAGGGTTT CCCCCAGACC AGAGGAATGG AGACAAGGAG 
TTTGTGATCC GCAGAGCAGC CACCAATCGT GTCTTGAACG TGCTCCGCCA CTGGGTGTCC 
AAGCACTCTC AGGACTTTGA GACCAACGAT GAGCTCAAAT GCAAGGTGAT CGGCTTCCTG 
GAAGAAGTCA TGCACGACCC GGAGCTCCTG ACCCAGGAGC GGAAGGCTGC AGCCAACATC 
ATCAGGACTC TGACCCAGGA GGACCCAGGT GACAACCAGA TCACGCTGGA GGAGATCACG 
CAGATGGCTG AAGGCGTGAA GGCTGAGCCC TTTGAAAACC ACTCAGCCCT GGAGATCGCG 
GAGCAGCTGA CCCTGCTAGA TCACCTCGTC TTCAAGAAGA TTCCTTATGA GGAGTTCTTC 
GGACAAGGAT GGATGAAACT GGAAAAGAAT GAAAGGACCC CTTATATCAT GAAAACCACT 
AAGCACTTCA ATGACATCAG TAACTTGATT GCTTCAGAAA TCATCCGCAA CGAGGACATC 
AACGCCAGGG TGAGCGCCAT CGAGAAGTGG GTGGCCGTAG CTGACATATG CCGCTGCCTC 
CACAACTACA ATGCCGTACT GGAGATCACC TCGTCCATGA ACCGCAGTGC AATCTTCCGG 
CTCAAAAAGA CGTGGCTCAA AGTCTCTAAG CAGACTAAAG CTTTGATTGA TAAGCTCCAA 
AAGCTTGTGT CATCTGAGGG CAGATTTAAG AATCTCAGAG AAGCTCTGAA AAATTGTGAC 
CCACCCTGTG TCCCTTACCT GGGGATGTAC CTCACCGACC TGGCCTTCAT CGAGGAGGGG 
ACGCCCAATT ACACGGAAGA CGGCCTGGTC AACTTCTCCA AGATGAGGAT GATATCCCAT 
ATTATCCGAG AGATTCGCCA GTTTCAACAA ACTGCCTACA AAATAGAGCA CCAAGCAAAG 
GTAACGCAAT ATTTACTGGA CCAATCTTTT GTAATGGATG AAGAAAGCCT CTACGAGTCT 
TCTCTCCGAA TAGAACCAAA ACTCCCCACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF1 ( XM_009429655.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009427930.1 Pan troglodytes Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X3, mRNA.

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CloneID	OPc68943
Clone ID Related Accession (Same CDS sequence)	XM_009429655.2
Accession Version	XM_009429655.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009429655.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC 
CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG 
GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG 
CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACGGAGA AGACCGTGGC CAAGCAGCTT 
CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCGCATCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC 
AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC 
ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCTG CCAGCTCCAA GAAGCCTCCC 
ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTGCAT 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT 
GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT 
CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA 
GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT 
ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC 
CAAGACACAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT 
ACGGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC 
AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TATATTGTGA TGATGTTGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGCTA CGCCAGTGTG 
GAGCGGCTGC TGGAGAGGCT GACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CCTACCGCGT CTTCACCACC GCCATCGTGG TCCTGGACAA GCTCATTACC 
ATCTACAAGA AGCCTATCAG TGCCATTCCT GCCAGGTGGC TGAGGTCGCT GGAGCTCCTG 
TTTGCCAGTG GCCAGAACAA TAAGCTCCTG TACGGTGAAC CCCCCAAGTC CCCGCGCGCC 
ACCCGCAAGT TCTCCTCGCC GCCACCTCTG TCCATCACCA AGACATCGTC ACCGAGCCGC 
CGGCGGAAGC TCTCCCTGAA CATCCCCATC ATCACCGGCG GCAAGGCCCT GGACCTGGCC 
GCCCTCAGCT GCAACTCCAA TGGCTACACC AGCATGTACT CGGCCATGTC GCCCTTCAGC 
AAGGCCACGC TGGACACCAG CAAGCTCTAT GTGTCCAGCA GCTTCACCAA CAAGATTCCA 
GATGAGGGCG ATACGACCCC TGAGAAGCCC GAAGACCCTT CGGCGCTCAG CAAGCAGAGC 
TCAGAAGTCT CCATGAGAGA GGAGTCAGAT ATTGATCAAA ACCAGAGTGA TGATGGTGAT 
ACTGAAACAT CACCAACTAA ATCTCCAACA ACACCCAAAT CAGTCAAAAG CAAAAATTCT 
TCAGAGTTCC CGCTCTTTTC CTATAACAAT GGAGTCGTCA TGACCTCCTG TCGTGAACTG 
GACAATAACC GCAGTGCCTT GTCGGCCGCC TCTGCCTTTG CCATAGCAAC CGCGGGGGCC 
AACGAGGGCA CCCCAAACAA GGAGAAGTAC CGGAGGATGT CCTTAGCCAG TGCAGGGTTT 
CCCCCAGACC AGAGGAATGG AGACAAGGAG TTTGTGATCC GCAGAGCAGC CACCAATCGT 
GTCTTGAACG TGCTCCGCCA CTGGGTGTCC AAGCACTCTC AGGACTTTGA GACCAACGAT 
GAGCTCAAAT GCAAGGTGAT CGGCTTCCTG GAAGAAGTCA TGCACGACCC GGAGCTCCTG 
ACCCAGGAGC GGAAGGCTGC AGCCAACATC ATCAGGACTC TGACCCAGGA GGACCCAGGT 
GACAACCAGA TCACGCTGGA GGAGATCACG CAGATGGCTG AAGGCGTGAA GGCTGAGCCC 
TTTGAAAACC ACTCAGCCCT GGAGATCGCG GAGCAGCTGA CCCTGCTAGA TCACCTCGTC 
TTCAAGAAGA TTCCTTATGA GGAGTTCTTC GGACAAGGAT GGATGAAACT GGAAAAGAAT 
GAAAGGACCC CTTATATCAT GAAAACCACT AAGCACTTCA ATGACATCAG TAACTTGATT 
GCTTCAGAAA TCATCCGCAA CGAGGACATC AACGCCAGGG TGAGCGCCAT CGAGAAGTGG 
GTGGCCGTAG CTGACATATG CCGCTGCCTC CACAACTACA ATGCCGTACT GGAGATCACC 
TCGTCCATGA ACCGCAGTGC AATCTTCCGG CTCAAAAAGA CGTGGCTCAA AGTCTCTAAG 
CAGACTAAAG CTTTGATTGA TAAGCTCCAA AAGCTTGTGT CATCTGAGGG CAGATTTAAG 
AATCTCAGAG AAGCTCTGAA AAATTGTGAC CCACCCTGTG TCCCTTACCT GGGGATGTAC 
CTCACCGACC TGGCCTTCAT CGAGGAGGGG ACGCCCAATT ACACGGAAGA CGGCCTGGTC 
AACTTCTCCA AGATGAGGAT GATATCCCAT ATTATCCGAG AGATTCGCCA GTTTCAACAA 
ACTGCCTACA AAATAGAGCA CCAAGCAAAG GTAACGCAAT ATTTACTGGA CCAATCTTTT 
GTAATGGATG AAGAAAGCCT CTACGAGTCT TCTCTCCGAA TAGAACCAAA ACTCCCCACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009427930.1
CDS	440..4222
Translation	MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEGCVCDRAPSPKPALSAKEPLEKQHYFTVNFSHENQKALELRTEDAKDCDEWVAAIAHASYRTLATEHEALMQKYLHLLQIVETEKTVAKQLRQQIEDGEIEIERLKAEIASLLKDNERIQSTQTVAPNDEDSDIKKIKKVQSFLRGWLCRRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIIEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLEEPESTEEEAKGSGQDTDHLDFKIGVEPKDSPPFTVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAIVVLDKLITIYKKPISAIPARWLRSLELLFASGQNNKLLYGEPPKSPRATRKFSSPPPLSITKTSSPSRRRKLSLNIPIITGGKALDLAALSCNSNGYTSMYSAMSPFSKATLDTSKLYVSSSFTNKIPDEGDTTPEKPEDPSALSKQSSEVSMREESDIDQNQSDDGDTETSPTKSPTTPKSVKSKNSSEFPLFSYNNGVVMTSCRELDNNRSALSAASAFAIATAGANEGTPNKEKYRRMSLASAGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELKCKVIGFLEEVMHDPELLTQERKAAANIIRTLTQEDPGDNQITLEEITQMAEGVKAEPFENHSALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDISNLIASEIIRNEDINARVSAIEKWVAVADICRCLHNYNAVLEITSSMNRSAIFRLKKTWLKVSKQTKALIDKLQKLVSSEGRFKNLREALKNCDPPCVPYLGMYLTDLAFIEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTAYKIEHQAKVTQYLLDQSFVMDEESLYESSLRIEPKLPT

Target ORF information:

RefSeq Version	XM_009429655.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009429655.2

ORF Insert Sequence:

ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC 
CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG 
GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG 
CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACGGAGA AGACCGTGGC CAAGCAGCTT 
CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCGCATCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC 
AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC 
ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCTG CCAGCTCCAA GAAGCCTCCC 
ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTGCAT 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT 
GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT 
CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA 
GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT 
ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC 
CAAGACACAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT 
ACGGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC 
AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TATATTGTGA TGATGTTGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGCTA CGCCAGTGTG 
GAGCGGCTGC TGGAGAGGCT GACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CCTACCGCGT CTTCACCACC GCCATCGTGG TCCTGGACAA GCTCATTACC 
ATCTACAAGA AGCCTATCAG TGCCATTCCT GCCAGGTGGC TGAGGTCGCT GGAGCTCCTG 
TTTGCCAGTG GCCAGAACAA TAAGCTCCTG TACGGTGAAC CCCCCAAGTC CCCGCGCGCC 
ACCCGCAAGT TCTCCTCGCC GCCACCTCTG TCCATCACCA AGACATCGTC ACCGAGCCGC 
CGGCGGAAGC TCTCCCTGAA CATCCCCATC ATCACCGGCG GCAAGGCCCT GGACCTGGCC 
GCCCTCAGCT GCAACTCCAA TGGCTACACC AGCATGTACT CGGCCATGTC GCCCTTCAGC 
AAGGCCACGC TGGACACCAG CAAGCTCTAT GTGTCCAGCA GCTTCACCAA CAAGATTCCA 
GATGAGGGCG ATACGACCCC TGAGAAGCCC GAAGACCCTT CGGCGCTCAG CAAGCAGAGC 
TCAGAAGTCT CCATGAGAGA GGAGTCAGAT ATTGATCAAA ACCAGAGTGA TGATGGTGAT 
ACTGAAACAT CACCAACTAA ATCTCCAACA ACACCCAAAT CAGTCAAAAG CAAAAATTCT 
TCAGAGTTCC CGCTCTTTTC CTATAACAAT GGAGTCGTCA TGACCTCCTG TCGTGAACTG 
GACAATAACC GCAGTGCCTT GTCGGCCGCC TCTGCCTTTG CCATAGCAAC CGCGGGGGCC 
AACGAGGGCA CCCCAAACAA GGAGAAGTAC CGGAGGATGT CCTTAGCCAG TGCAGGGTTT 
CCCCCAGACC AGAGGAATGG AGACAAGGAG TTTGTGATCC GCAGAGCAGC CACCAATCGT 
GTCTTGAACG TGCTCCGCCA CTGGGTGTCC AAGCACTCTC AGGACTTTGA GACCAACGAT 
GAGCTCAAAT GCAAGGTGAT CGGCTTCCTG GAAGAAGTCA TGCACGACCC GGAGCTCCTG 
ACCCAGGAGC GGAAGGCTGC AGCCAACATC ATCAGGACTC TGACCCAGGA GGACCCAGGT 
GACAACCAGA TCACGCTGGA GGAGATCACG CAGATGGCTG AAGGCGTGAA GGCTGAGCCC 
TTTGAAAACC ACTCAGCCCT GGAGATCGCG GAGCAGCTGA CCCTGCTAGA TCACCTCGTC 
TTCAAGAAGA TTCCTTATGA GGAGTTCTTC GGACAAGGAT GGATGAAACT GGAAAAGAAT 
GAAAGGACCC CTTATATCAT GAAAACCACT AAGCACTTCA ATGACATCAG TAACTTGATT 
GCTTCAGAAA TCATCCGCAA CGAGGACATC AACGCCAGGG TGAGCGCCAT CGAGAAGTGG 
GTGGCCGTAG CTGACATATG CCGCTGCCTC CACAACTACA ATGCCGTACT GGAGATCACC 
TCGTCCATGA ACCGCAGTGC AATCTTCCGG CTCAAAAAGA CGTGGCTCAA AGTCTCTAAG 
CAGACTAAAG CTTTGATTGA TAAGCTCCAA AAGCTTGTGT CATCTGAGGG CAGATTTAAG 
AATCTCAGAG AAGCTCTGAA AAATTGTGAC CCACCCTGTG TCCCTTACCT GGGGATGTAC 
CTCACCGACC TGGCCTTCAT CGAGGAGGGG ACGCCCAATT ACACGGAAGA CGGCCTGGTC 
AACTTCTCCA AGATGAGGAT GATATCCCAT ATTATCCGAG AGATTCGCCA GTTTCAACAA 
ACTGCCTACA AAATAGAGCA CCAAGCAAAG GTAACGCAAT ATTTACTGGA CCAATCTTTT 
GTAATGGATG AAGAAAGCCT CTACGAGTCT TCTCTCCGAA TAGAACCAAA ACTCCCCACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF1 ( XM_001153395.4 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_001153395.2 Pan troglodytes Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X4, mRNA.

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CloneID	OPc68944
Clone ID Related Accession (Same CDS sequence)	XM_001153395.4
Accession Version	XM_001153395.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036894.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_001153395.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC 
CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG 
GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG 
CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACGGAGA AGACCGTGGC CAAGCAGCTT 
CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCGCATCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC 
AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC 
ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCTG CCAGCTCCAA GAAGCCTCCC 
ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTGCAT 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT 
GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT 
CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA 
GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT 
ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC 
CAAGACACAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT 
ACGGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC 
AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TATATTGTGA TGATGTTGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGCTA CGCCAGTGTG 
GAGCGGCTGC TGGAGAGGCT GACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CCTACCGCGT CTTCACCACC GCCATCGTGG TCCTGGACAA GCTCATTACC 
ATCTACAAGA AGCCTATCAG TGCCATTCCT GCCAGGTCGC TGGAGCTCCT GTTTGCCAGT 
GGCCAGAACA ATAAGCTCCT GTACGGTGAA CCCCCCAAGT CCCCGCGCGC CACCCGCAAG 
TTCTCCTCGC CGCCACCTCT GTCCATCACC AAGACATCGT CACCGAGCCG CCGGCGGAAG 
CTCTCCCTGA ACATCCCCAT CATCACCGGC GGCAAGGCCC TGGACCTGGC CGCCCTCAGC 
TGCAACTCCA ATGGCTACAC CAGCATGTAC TCGGCCATGT CGCCCTTCAG CAAGGCCACG 
CTGGACACCA GCAAGCTCTA TGTGTCCAGC AGCTTCACCA ACAAGATTCC AGATGAGGGC 
GATACGACCC CTGAGAAGCC CGAAGACCCT TCGGCGCTCA GCAAGCAGAG CTCAGAAGTC 
TCCATGAGAG AGGAGTCAGA TATTGATCAA AACCAGAGTG ATGATGGTGA TACTGAAACA 
TCACCAACTA AATCTCCAAC AACACCCAAA TCAGTCAAAA GCAAAAATTC TTCAGAGTTC 
CCGCTCTTTT CCTATAACAA TGGAGTCGTC ATGACCTCCT GTCGTGAACT GGACAATAAC 
CGCAGTGCCT TGTCGGCCGC CTCTGCCTTT GCCATAGCAA CCGCGGGGGC CAACGAGGGC 
ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTAGCCA GTGCAGGGTT TCCCCCAGAC 
CAGAGGAATG GAGACAAGGA GTTTGTGATC CGCAGAGCAG CCACCAATCG TGTCTTGAAC 
GTGCTCCGCC ACTGGGTGTC CAAGCACTCT CAGGACTTTG AGACCAACGA TGAGCTCAAA 
TGCAAGGTGA TCGGCTTCCT GGAAGAAGTC ATGCACGACC CGGAGCTCCT GACCCAGGAG 
CGGAAGGCTG CAGCCAACAT CATCAGGACT CTGACCCAGG AGGACCCAGG TGACAACCAG 
ATCACGCTGG AGGAGATCAC GCAGATGGCT GAAGGCGTGA AGGCTGAGCC CTTTGAAAAC 
CACTCAGCCC TGGAGATCGC GGAGCAGCTG ACCCTGCTAG ATCACCTCGT CTTCAAGAAG 
ATTCCTTATG AGGAGTTCTT CGGACAAGGA TGGATGAAAC TGGAAAAGAA TGAAAGGACC 
CCTTATATCA TGAAAACCAC TAAGCACTTC AATGACATCA GTAACTTGAT TGCTTCAGAA 
ATCATCCGCA ACGAGGACAT CAACGCCAGG GTGAGCGCCA TCGAGAAGTG GGTGGCCGTA 
GCTGACATAT GCCGCTGCCT CCACAACTAC AATGCCGTAC TGGAGATCAC CTCGTCCATG 
AACCGCAGTG CAATCTTCCG GCTCAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGACTAAA 
GCTTTGATTG ATAAGCTCCA AAAGCTTGTG TCATCTGAGG GCAGATTTAA GAATCTCAGA 
GAAGCTCTGA AAAATTGTGA CCCACCCTGT GTCCCTTACC TGGGGATGTA CCTCACCGAC 
CTGGCCTTCA TCGAGGAGGG GACGCCCAAT TACACGGAAG ACGGCCTGGT CAACTTCTCC 
AAGATGAGGA TGATATCCCA TATTATCCGA GAGATTCGCC AGTTTCAACA AACTGCCTAC 
AAAATAGAGC ACCAAGCAAA GGTAACGCAA TATTTACTGG ACCAATCTTT TGTAATGGAT 
GAAGAAAGCC TCTACGAGTC TTCTCTCCGA ATAGAACCAA AACTCCCCAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001153395.2
CDS	376..4149
Translation	MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEGCVCDRAPSPKPALSAKEPLEKQHYFTVNFSHENQKALELRTEDAKDCDEWVAAIAHASYRTLATEHEALMQKYLHLLQIVETEKTVAKQLRQQIEDGEIEIERLKAEIASLLKDNERIQSTQTVAPNDEDSDIKKIKKVQSFLRGWLCRRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIIEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLEEPESTEEEAKGSGQDTDHLDFKIGVEPKDSPPFTVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAIVVLDKLITIYKKPISAIPARSLELLFASGQNNKLLYGEPPKSPRATRKFSSPPPLSITKTSSPSRRRKLSLNIPIITGGKALDLAALSCNSNGYTSMYSAMSPFSKATLDTSKLYVSSSFTNKIPDEGDTTPEKPEDPSALSKQSSEVSMREESDIDQNQSDDGDTETSPTKSPTTPKSVKSKNSSEFPLFSYNNGVVMTSCRELDNNRSALSAASAFAIATAGANEGTPNKEKYRRMSLASAGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELKCKVIGFLEEVMHDPELLTQERKAAANIIRTLTQEDPGDNQITLEEITQMAEGVKAEPFENHSALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDISNLIASEIIRNEDINARVSAIEKWVAVADICRCLHNYNAVLEITSSMNRSAIFRLKKTWLKVSKQTKALIDKLQKLVSSEGRFKNLREALKNCDPPCVPYLGMYLTDLAFIEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTAYKIEHQAKVTQYLLDQSFVMDEESLYESSLRIEPKLPT

Target ORF information:

RefSeq Version	XM_001153395.4
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001153395.4

ORF Insert Sequence:

ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC 
CATGAGAACC AGAAAGCCTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG 
GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTCGCCA CAGAGCATGA GGCATTAATG 
CAGAAATACC TGCACCTGCT GCAGATCGTG GAGACGGAGA AGACCGTGGC CAAGCAGCTT 
CGGCAGCAGA TCGAGGATGG GGAGATCGAG ATCGAGCGGC TGAAGGCAGA GATCGCATCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAA CGATGAAGAC 
AGCGACATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG 
AAGTGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCTGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC 
ATCCTTGTCA ACAATTTCCT GCGCCCGCTG CGGATGGCTG CCAGCTCCAA GAAGCCTCCC 
ATCACACACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTGCAT 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT 
GACCTATTTG ACATCCTGCT GCCCATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTCGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT 
CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA 
GGTTCCCTCA TTCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCTCTCTCCC TAAAGAAAGA GGGCGAGCGA CAGTGCTTCC TGTTTTCTAA GCATCTGATT 
ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACTTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC 
CAAGACACAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT 
ACGGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC 
AAGGTCACTG TGCCGCAGAT GATCAAGTCC GACGCCTCCT TATATTGTGA TGATGTTGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGCTA CGCCAGTGTG 
GAGCGGCTGC TGGAGAGGCT GACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CCTACCGCGT CTTCACCACC GCCATCGTGG TCCTGGACAA GCTCATTACC 
ATCTACAAGA AGCCTATCAG TGCCATTCCT GCCAGGTCGC TGGAGCTCCT GTTTGCCAGT 
GGCCAGAACA ATAAGCTCCT GTACGGTGAA CCCCCCAAGT CCCCGCGCGC CACCCGCAAG 
TTCTCCTCGC CGCCACCTCT GTCCATCACC AAGACATCGT CACCGAGCCG CCGGCGGAAG 
CTCTCCCTGA ACATCCCCAT CATCACCGGC GGCAAGGCCC TGGACCTGGC CGCCCTCAGC 
TGCAACTCCA ATGGCTACAC CAGCATGTAC TCGGCCATGT CGCCCTTCAG CAAGGCCACG 
CTGGACACCA GCAAGCTCTA TGTGTCCAGC AGCTTCACCA ACAAGATTCC AGATGAGGGC 
GATACGACCC CTGAGAAGCC CGAAGACCCT TCGGCGCTCA GCAAGCAGAG CTCAGAAGTC 
TCCATGAGAG AGGAGTCAGA TATTGATCAA AACCAGAGTG ATGATGGTGA TACTGAAACA 
TCACCAACTA AATCTCCAAC AACACCCAAA TCAGTCAAAA GCAAAAATTC TTCAGAGTTC 
CCGCTCTTTT CCTATAACAA TGGAGTCGTC ATGACCTCCT GTCGTGAACT GGACAATAAC 
CGCAGTGCCT TGTCGGCCGC CTCTGCCTTT GCCATAGCAA CCGCGGGGGC CAACGAGGGC 
ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTAGCCA GTGCAGGGTT TCCCCCAGAC 
CAGAGGAATG GAGACAAGGA GTTTGTGATC CGCAGAGCAG CCACCAATCG TGTCTTGAAC 
GTGCTCCGCC ACTGGGTGTC CAAGCACTCT CAGGACTTTG AGACCAACGA TGAGCTCAAA 
TGCAAGGTGA TCGGCTTCCT GGAAGAAGTC ATGCACGACC CGGAGCTCCT GACCCAGGAG 
CGGAAGGCTG CAGCCAACAT CATCAGGACT CTGACCCAGG AGGACCCAGG TGACAACCAG 
ATCACGCTGG AGGAGATCAC GCAGATGGCT GAAGGCGTGA AGGCTGAGCC CTTTGAAAAC 
CACTCAGCCC TGGAGATCGC GGAGCAGCTG ACCCTGCTAG ATCACCTCGT CTTCAAGAAG 
ATTCCTTATG AGGAGTTCTT CGGACAAGGA TGGATGAAAC TGGAAAAGAA TGAAAGGACC 
CCTTATATCA TGAAAACCAC TAAGCACTTC AATGACATCA GTAACTTGAT TGCTTCAGAA 
ATCATCCGCA ACGAGGACAT CAACGCCAGG GTGAGCGCCA TCGAGAAGTG GGTGGCCGTA 
GCTGACATAT GCCGCTGCCT CCACAACTAC AATGCCGTAC TGGAGATCAC CTCGTCCATG 
AACCGCAGTG CAATCTTCCG GCTCAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGACTAAA 
GCTTTGATTG ATAAGCTCCA AAAGCTTGTG TCATCTGAGG GCAGATTTAA GAATCTCAGA 
GAAGCTCTGA AAAATTGTGA CCCACCCTGT GTCCCTTACC TGGGGATGTA CCTCACCGAC 
CTGGCCTTCA TCGAGGAGGG GACGCCCAAT TACACGGAAG ACGGCCTGGT CAACTTCTCC 
AAGATGAGGA TGATATCCCA TATTATCCGA GAGATTCGCC AGTTTCAACA AACTGCCTAC 
AAAATAGAGC ACCAAGCAAA GGTAACGCAA TATTTACTGG ACCAATCTTT TGTAATGGAT 
GAAGAAAGCC TCTACGAGTC TTCTCTCCGA ATAGAACCAA AACTCCCCAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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RASGRF1 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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