MID2 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene
Clones
gRNAs

Summary

Gene Symbol	MID2
Entrez Gene ID	107971018
Full Name	midline 2
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016942890.2	XP_016798379.1	probable E3 ubiquitin-protein ligase MID2 isoform X1
XM_016942890.1	XP_016798379.1	probable E3 ubiquitin-protein ligase MID2 isoform X1
XM_016942892.1	XP_016798381.1	probable E3 ubiquitin-protein ligase MID2 isoform X3
XM_016942891.2	XP_016798380.1	probable E3 ubiquitin-protein ligase MID2 isoform X2
XM_016942891.1	XP_016798380.1	probable E3 ubiquitin-protein ligase MID2 isoform X2
XM_016942892.2	XP_016798381.1	probable E3 ubiquitin-protein ligase MID2 isoform X3

Pathways from BioSystems
Gene Ontology

The following MID2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MID2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc25146	XM_016942891.2 Latest version!	Pan troglodytes midline 2 (MID2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OPc25147	XM_016942890.2 Latest version!	Pan troglodytes midline 2 (MID2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OPc42485	XM_016942892.2 Latest version!	Pan troglodytes midline 2 (MID2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
OPc25147	XM_016942890.1	Pan troglodytes midline 2 (MID2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OPc25146	XM_016942891.1	Pan troglodytes midline 2 (MID2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OPc42485	XM_016942892.1	Pan troglodytes midline 2 (MID2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MID2 ( XM_016942891.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016798380.1 Pan troglodytes midline 2 (MID2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPc25146
Clone ID Related Accession (Same CDS sequence)	XM_016942891.2 , XM_016942891.1
Accession Version	XM_016942891.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036902.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016942891.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAAA GCCCAGCCTC CGTGGTTCTT AATGCCTCAG GAGGACTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CACTGAACCA CCGGGGTCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TTATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TATAATTCAG TAGACAGCTG GATGATTGTT 
CCCAACATTA AACAGAACCA TTACACAGTG CATGGACTCC AGAGCGGGAC TCGCTACATC 
TTCATCGTTA AAGCCATAAA CCAAGCCGGC AGCCGGAACA GTGAACCTAC CCGACTAAAA 
ACAAACAGCC AACCCTTTAA ATTGGATCCC AAAATGACTC ACAAGAAGTT GAAGATTTCC 
AATGATGGAT TGCAGATGGA GAAGGATGAA AGCTCTCTAA AGAAGAGCCA CACCCCAGAG 
AGGTTTAGTG GCACAGGGTG CTATGGGGCA GCAGGAAATA TATTCATTGA CAGTGGCTGC 
CACTATTGGG AGGTGGTCAT GGGTTCCTCA ACATGGTATG CAATTGGCAT TGCCTACAAA 
TCAGCTCCAA AGAATGAATG GATTGGCAAG AATGCCTCCT CATGGGTCTT CTCTCGCTGC 
AATAGTAACT TCGTGGTGAG ACACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAC 
CTGAAGCGTC TGGGTGTCCT CCTGGATTAT GACAACAATA TGCTGTCTTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTGACCTTCA TTCTTCCAGT TTGTCCAACA 
TTTACAATCT GGAACAAATC CCTAATGATC CTGTCTGGCT TGCCTGCCCC AGATTTTATT 
GATTACCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTTTCTGGG 
ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016798380.1
CDS	588..2705
Translation	MGESPASVVLNASGGLFSLKMETLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPTTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPHLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKTCH

Target ORF information:

RefSeq Version	XM_016942891.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes midline 2 (MID2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016942891.2

ORF Insert Sequence:

ATGGGTGAAA GCCCAGCCTC CGTGGTTCTT AATGCCTCAG GAGGACTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CACTGAACCA CCGGGGTCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TTATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TATAATTCAG TAGACAGCTG GATGATTGTT 
CCCAACATTA AACAGAACCA TTACACAGTG CATGGACTCC AGAGCGGGAC TCGCTACATC 
TTCATCGTTA AAGCCATAAA CCAAGCCGGC AGCCGGAACA GTGAACCTAC CCGACTAAAA 
ACAAACAGCC AACCCTTTAA ATTGGATCCC AAAATGACTC ACAAGAAGTT GAAGATTTCC 
AATGATGGAT TGCAGATGGA GAAGGATGAA AGCTCTCTAA AGAAGAGCCA CACCCCAGAG 
AGGTTTAGTG GCACAGGGTG CTATGGGGCA GCAGGAAATA TATTCATTGA CAGTGGCTGC 
CACTATTGGG AGGTGGTCAT GGGTTCCTCA ACATGGTATG CAATTGGCAT TGCCTACAAA 
TCAGCTCCAA AGAATGAATG GATTGGCAAG AATGCCTCCT CATGGGTCTT CTCTCGCTGC 
AATAGTAACT TCGTGGTGAG ACACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAC 
CTGAAGCGTC TGGGTGTCCT CCTGGATTAT GACAACAATA TGCTGTCTTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTGACCTTCA TTCTTCCAGT TTGTCCAACA 
TTTACAATCT GGAACAAATC CCTAATGATC CTGTCTGGCT TGCCTGCCCC AGATTTTATT 
GATTACCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTTTCTGGG 
ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_016942891.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016798380.1 Pan troglodytes midline 2 (MID2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPc25146
Clone ID Related Accession (Same CDS sequence)	XM_016942891.2 , XM_016942891.1
Accession Version	XM_016942891.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006491.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAAA GCCCAGCCTC CGTGGTTCTT AATGCCTCAG GAGGACTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CACTGAACCA CCGGGGTCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TTATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TATAATTCAG TAGACAGCTG GATGATTGTT 
CCCAACATTA AACAGAACCA TTACACAGTG CATGGACTCC AGAGCGGGAC TCGCTACATC 
TTCATCGTTA AAGCCATAAA CCAAGCCGGC AGCCGGAACA GTGAACCTAC CCGACTAAAA 
ACAAACAGCC AACCCTTTAA ATTGGATCCC AAAATGACTC ACAAGAAGTT GAAGATTTCC 
AATGATGGAT TGCAGATGGA GAAGGATGAA AGCTCTCTAA AGAAGAGCCA CACCCCAGAG 
AGGTTTAGTG GCACAGGGTG CTATGGGGCA GCAGGAAATA TATTCATTGA CAGTGGCTGC 
CACTATTGGG AGGTGGTCAT GGGTTCCTCA ACATGGTATG CAATTGGCAT TGCCTACAAA 
TCAGCTCCAA AGAATGAATG GATTGGCAAG AATGCCTCCT CATGGGTCTT CTCTCGCTGC 
AATAGTAACT TCGTGGTGAG ACACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAC 
CTGAAGCGTC TGGGTGTCCT CCTGGATTAT GACAACAATA TGCTGTCTTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTGACCTTCA TTCTTCCAGT TTGTCCAACA 
TTTACAATCT GGAACAAATC CCTAATGATC CTGTCTGGCT TGCCTGCCCC AGATTTTATT 
GATTACCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTTTCTGGG 
ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016798380.1
CDS	591..2708
Translation	MGESPASVVLNASGGLFSLKMETLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPTTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPHLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKTCH

Target ORF information:

RefSeq Version	XM_016942891.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes midline 2 (MID2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016942891.1

ORF Insert Sequence:

ATGGGTGAAA GCCCAGCCTC CGTGGTTCTT AATGCCTCAG GAGGACTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CACTGAACCA CCGGGGTCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TTATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TATAATTCAG TAGACAGCTG GATGATTGTT 
CCCAACATTA AACAGAACCA TTACACAGTG CATGGACTCC AGAGCGGGAC TCGCTACATC 
TTCATCGTTA AAGCCATAAA CCAAGCCGGC AGCCGGAACA GTGAACCTAC CCGACTAAAA 
ACAAACAGCC AACCCTTTAA ATTGGATCCC AAAATGACTC ACAAGAAGTT GAAGATTTCC 
AATGATGGAT TGCAGATGGA GAAGGATGAA AGCTCTCTAA AGAAGAGCCA CACCCCAGAG 
AGGTTTAGTG GCACAGGGTG CTATGGGGCA GCAGGAAATA TATTCATTGA CAGTGGCTGC 
CACTATTGGG AGGTGGTCAT GGGTTCCTCA ACATGGTATG CAATTGGCAT TGCCTACAAA 
TCAGCTCCAA AGAATGAATG GATTGGCAAG AATGCCTCCT CATGGGTCTT CTCTCGCTGC 
AATAGTAACT TCGTGGTGAG ACACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAC 
CTGAAGCGTC TGGGTGTCCT CCTGGATTAT GACAACAATA TGCTGTCTTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTGACCTTCA TTCTTCCAGT TTGTCCAACA 
TTTACAATCT GGAACAAATC CCTAATGATC CTGTCTGGCT TGCCTGCCCC AGATTTTATT 
GATTACCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTTTCTGGG 
ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_016942890.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016798379.1 Pan troglodytes midline 2 (MID2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPc25147
Clone ID Related Accession (Same CDS sequence)	XM_016942890.2 , XM_016942890.1
Accession Version	XM_016942890.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036902.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016942890.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAAA GCCCAGCCTC CGTGGTTCTT AATGCCTCAG GAGGACTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CACTGAACCA CCGGGGTCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TTATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TATAATTCAG TAGACAGCTG GATGATTGTT CCCAACATTA AACAGAACCA TTACACAGTG 
CATGGACTCC AGAGCGGGAC TCGCTACATC TTCATCGTTA AAGCCATAAA CCAAGCCGGC 
AGCCGGAACA GTGAACCTAC CCGACTAAAA ACAAACAGCC AACCCTTTAA ATTGGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATTTCC AATGATGGAT TGCAGATGGA GAAGGATGAA 
AGCTCTCTAA AGAAGAGCCA CACCCCAGAG AGGTTTAGTG GCACAGGGTG CTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTATTGGG AGGTGGTCAT GGGTTCCTCA 
ACATGGTATG CAATTGGCAT TGCCTACAAA TCAGCTCCAA AGAATGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGCTGC AATAGTAACT TCGTGGTGAG ACACAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAC CTGAAGCGTC TGGGTGTCCT CCTGGATTAT 
GACAACAATA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTTACAATCT GGAACAAATC CCTAATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016798379.1
CDS	591..2798
Translation	MGESPASVVLNASGGLFSLKMETLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPTTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPHLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKTCH

Target ORF information:

RefSeq Version	XM_016942890.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes midline 2 (MID2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016942890.2

ORF Insert Sequence:

ATGGGTGAAA GCCCAGCCTC CGTGGTTCTT AATGCCTCAG GAGGACTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CACTGAACCA CCGGGGTCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TTATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TATAATTCAG TAGACAGCTG GATGATTGTT CCCAACATTA AACAGAACCA TTACACAGTG 
CATGGACTCC AGAGCGGGAC TCGCTACATC TTCATCGTTA AAGCCATAAA CCAAGCCGGC 
AGCCGGAACA GTGAACCTAC CCGACTAAAA ACAAACAGCC AACCCTTTAA ATTGGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATTTCC AATGATGGAT TGCAGATGGA GAAGGATGAA 
AGCTCTCTAA AGAAGAGCCA CACCCCAGAG AGGTTTAGTG GCACAGGGTG CTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTATTGGG AGGTGGTCAT GGGTTCCTCA 
ACATGGTATG CAATTGGCAT TGCCTACAAA TCAGCTCCAA AGAATGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGCTGC AATAGTAACT TCGTGGTGAG ACACAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAC CTGAAGCGTC TGGGTGTCCT CCTGGATTAT 
GACAACAATA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTTACAATCT GGAACAAATC CCTAATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_016942890.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016798379.1 Pan troglodytes midline 2 (MID2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPc25147
Clone ID Related Accession (Same CDS sequence)	XM_016942890.2 , XM_016942890.1
Accession Version	XM_016942890.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006491.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAAA GCCCAGCCTC CGTGGTTCTT AATGCCTCAG GAGGACTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CACTGAACCA CCGGGGTCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TTATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TATAATTCAG TAGACAGCTG GATGATTGTT CCCAACATTA AACAGAACCA TTACACAGTG 
CATGGACTCC AGAGCGGGAC TCGCTACATC TTCATCGTTA AAGCCATAAA CCAAGCCGGC 
AGCCGGAACA GTGAACCTAC CCGACTAAAA ACAAACAGCC AACCCTTTAA ATTGGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATTTCC AATGATGGAT TGCAGATGGA GAAGGATGAA 
AGCTCTCTAA AGAAGAGCCA CACCCCAGAG AGGTTTAGTG GCACAGGGTG CTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTATTGGG AGGTGGTCAT GGGTTCCTCA 
ACATGGTATG CAATTGGCAT TGCCTACAAA TCAGCTCCAA AGAATGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGCTGC AATAGTAACT TCGTGGTGAG ACACAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAC CTGAAGCGTC TGGGTGTCCT CCTGGATTAT 
GACAACAATA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTTACAATCT GGAACAAATC CCTAATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016798379.1
CDS	593..2800
Translation	MGESPASVVLNASGGLFSLKMETLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPTTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPHLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKTCH

Target ORF information:

RefSeq Version	XM_016942890.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes midline 2 (MID2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016942890.1

ORF Insert Sequence:

ATGGGTGAAA GCCCAGCCTC CGTGGTTCTT AATGCCTCAG GAGGACTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CACTGAACCA CCGGGGTCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TTATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TATAATTCAG TAGACAGCTG GATGATTGTT CCCAACATTA AACAGAACCA TTACACAGTG 
CATGGACTCC AGAGCGGGAC TCGCTACATC TTCATCGTTA AAGCCATAAA CCAAGCCGGC 
AGCCGGAACA GTGAACCTAC CCGACTAAAA ACAAACAGCC AACCCTTTAA ATTGGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATTTCC AATGATGGAT TGCAGATGGA GAAGGATGAA 
AGCTCTCTAA AGAAGAGCCA CACCCCAGAG AGGTTTAGTG GCACAGGGTG CTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTATTGGG AGGTGGTCAT GGGTTCCTCA 
ACATGGTATG CAATTGGCAT TGCCTACAAA TCAGCTCCAA AGAATGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGCTGC AATAGTAACT TCGTGGTGAG ACACAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAC CTGAAGCGTC TGGGTGTCCT CCTGGATTAT 
GACAACAATA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTTACAATCT GGAACAAATC CCTAATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_016942892.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016798381.1 Pan troglodytes midline 2 (MID2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPc42485
Clone ID Related Accession (Same CDS sequence)	XM_016942892.1 , XM_016942892.2
Accession Version	XM_016942892.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006491.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CACTGAACCA CCGGGGTCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TTATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TATAATTCAG TAGACAGCTG GATGATTGTT CCCAACATTA AACAGAACCA TTACACAGTG 
CATGGACTCC AGAGCGGGAC TCGCTACATC TTCATCGTTA AAGCCATAAA CCAAGCCGGC 
AGCCGGAACA GTGAACCTAC CCGACTAAAA ACAAACAGCC AACCCTTTAA ATTGGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATTTCC AATGATGGAT TGCAGATGGA GAAGGATGAA 
AGCTCTCTAA AGAAGAGCCA CACCCCAGAG AGGTTTAGTG GCACAGGGTG CTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTATTGGG AGGTGGTCAT GGGTTCCTCA 
ACATGGTATG CAATTGGCAT TGCCTACAAA TCAGCTCCAA AGAATGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGCTGC AATAGTAACT TCGTGGTGAG ACACAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAC CTGAAGCGTC TGGGTGTCCT CCTGGATTAT 
GACAACAATA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTTACAATCT GGAACAAATC CCTAATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016798381.1
CDS	197..2344
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPTTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPHLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKTCH

Target ORF information:

RefSeq Version	XM_016942892.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes midline 2 (MID2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016942892.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CACTGAACCA CCGGGGTCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TTATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TATAATTCAG TAGACAGCTG GATGATTGTT CCCAACATTA AACAGAACCA TTACACAGTG 
CATGGACTCC AGAGCGGGAC TCGCTACATC TTCATCGTTA AAGCCATAAA CCAAGCCGGC 
AGCCGGAACA GTGAACCTAC CCGACTAAAA ACAAACAGCC AACCCTTTAA ATTGGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATTTCC AATGATGGAT TGCAGATGGA GAAGGATGAA 
AGCTCTCTAA AGAAGAGCCA CACCCCAGAG AGGTTTAGTG GCACAGGGTG CTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTATTGGG AGGTGGTCAT GGGTTCCTCA 
ACATGGTATG CAATTGGCAT TGCCTACAAA TCAGCTCCAA AGAATGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGCTGC AATAGTAACT TCGTGGTGAG ACACAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAC CTGAAGCGTC TGGGTGTCCT CCTGGATTAT 
GACAACAATA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTTACAATCT GGAACAAATC CCTAATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_016942892.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016798381.1 Pan troglodytes midline 2 (MID2), transcript variant X3, mRNA.

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CloneID	OPc42485
Clone ID Related Accession (Same CDS sequence)	XM_016942892.1 , XM_016942892.2
Accession Version	XM_016942892.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036902.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016942892.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CACTGAACCA CCGGGGTCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TTATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TATAATTCAG TAGACAGCTG GATGATTGTT CCCAACATTA AACAGAACCA TTACACAGTG 
CATGGACTCC AGAGCGGGAC TCGCTACATC TTCATCGTTA AAGCCATAAA CCAAGCCGGC 
AGCCGGAACA GTGAACCTAC CCGACTAAAA ACAAACAGCC AACCCTTTAA ATTGGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATTTCC AATGATGGAT TGCAGATGGA GAAGGATGAA 
AGCTCTCTAA AGAAGAGCCA CACCCCAGAG AGGTTTAGTG GCACAGGGTG CTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTATTGGG AGGTGGTCAT GGGTTCCTCA 
ACATGGTATG CAATTGGCAT TGCCTACAAA TCAGCTCCAA AGAATGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGCTGC AATAGTAACT TCGTGGTGAG ACACAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAC CTGAAGCGTC TGGGTGTCCT CCTGGATTAT 
GACAACAATA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTTACAATCT GGAACAAATC CCTAATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016798381.1
CDS	195..2342
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPTTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPHLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKTCH

Target ORF information:

RefSeq Version	XM_016942892.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes midline 2 (MID2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016942892.2

ORF Insert Sequence:

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CACTGAACCA CCGGGGTCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TTATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TATAATTCAG TAGACAGCTG GATGATTGTT CCCAACATTA AACAGAACCA TTACACAGTG 
CATGGACTCC AGAGCGGGAC TCGCTACATC TTCATCGTTA AAGCCATAAA CCAAGCCGGC 
AGCCGGAACA GTGAACCTAC CCGACTAAAA ACAAACAGCC AACCCTTTAA ATTGGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATTTCC AATGATGGAT TGCAGATGGA GAAGGATGAA 
AGCTCTCTAA AGAAGAGCCA CACCCCAGAG AGGTTTAGTG GCACAGGGTG CTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTATTGGG AGGTGGTCAT GGGTTCCTCA 
ACATGGTATG CAATTGGCAT TGCCTACAAA TCAGCTCCAA AGAATGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGCTGC AATAGTAACT TCGTGGTGAG ACACAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAC CTGAAGCGTC TGGGTGTCCT CCTGGATTAT 
GACAACAATA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTTACAATCT GGAACAAATC CCTAATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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MID2 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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