MANF cDNA ORF clone, Pan paniscus(pygmy chimpanzee)

Gene
Clones
gRNAs

Summary

Gene Symbol	MANF
Entrez Gene ID	100968261
Full Name	mesencephalic astrocyte derived neurotrophic factor
Gene Type	protein-coding
Organism	Pan paniscus(pygmy chimpanzee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003818799.2	XP_003818847.2	mesencephalic astrocyte-derived neurotrophic factor

Pathways from BioSystems
Gene Ontology

The following MANF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MANF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa46690	XM_003818799.2 Latest version!	Pan paniscus mesencephalic astrocyte derived neurotrophic factor (MANF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MANF ( XM_003818799.2 ) cDNA ORF clone, Pan paniscus(pygmy chimpanzee) -> XP_003818847.2 Pan paniscus mesencephalic astrocyte derived neurotrophic factor (MANF), mRNA.

Price & Availability↑

CloneID	OPa46690
Clone ID Related Accession (Same CDS sequence)	XM_003818799.2
Accession Version	XM_003818799.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-30
Organism	Pan paniscus(pygmy chimpanzee)
Product	mesencephalic astrocyte-derived neurotrophic factor
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_027871.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 2, 2014 this sequence version replaced XM_003818799.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan paniscus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGGA TGTGGGCCAC GCAGGGGCTG GCGGTGGCGC TGGCTCTGAG CGTGCTGCCG 
GGCAGCCGGT CGCTGCGGCC GGGCGACTGC GAAGTTTGTA TTTCTTATCT GGGAAGATTT 
TACCAGGACC TCAAAGACAG AGATGTCACA TTCTCACCAG CCACTATTGA AAACGAACTT 
ATAAAGTTCT GCCGGGAAGC AAGAGGCAAA GAGAATCGGT TGTGCTACTA TATCGGGGCC 
ACAGATGATG CAGCCACCAA AATCATCAAT GAGGTATCAA AGCCTCTGGC CCACCACATC 
CCTGTGGAGA AGATCTGTGA GAAGCTTAAG AAGAAGGACA GCCAGATATG TGAGCTAAAG 
TATGACAAGC AGATTGACCT GAGCACAGTG GACCTGAAGA AGCTCCGAGT TAAAGAGCTG 
AAGAAGATTC TGGATGACTG GGGGGAGACA TGCAAAGGCT GTGCAGAAAA GTCTGACTAC 
ATCCGGAAGA TAAATGAACT GATGCCTAAA TATGCCCCCA AGGCAGCCAG TGCACGGACC 
GATTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003818847.2
CDS	87..635
Translation	MRRMWATQGLAVALALSVLPGSRSLRPGDCEVCISYLGRFYQDLKDRDVTFSPATIENELIKFCREARGKENRLCYYIGATDDAATKIINEVSKPLAHHIPVEKICEKLKKKDSQICELKYDKQIDLSTVDLKKLRVKELKKILDDWGETCKGCAEKSDYIRKINELMPKYAPKAASARTDL

Target ORF information:

RefSeq Version	XM_003818799.2
Organism	Pan paniscus(pygmy chimpanzee)
Definition	Pan paniscus mesencephalic astrocyte derived neurotrophic factor (MANF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003818799.2

ORF Insert Sequence:

ATGAGGAGGA TGTGGGCCAC GCAGGGGCTG GCGGTGGCGC TGGCTCTGAG CGTGCTGCCG 
GGCAGCCGGT CGCTGCGGCC GGGCGACTGC GAAGTTTGTA TTTCTTATCT GGGAAGATTT 
TACCAGGACC TCAAAGACAG AGATGTCACA TTCTCACCAG CCACTATTGA AAACGAACTT 
ATAAAGTTCT GCCGGGAAGC AAGAGGCAAA GAGAATCGGT TGTGCTACTA TATCGGGGCC 
ACAGATGATG CAGCCACCAA AATCATCAAT GAGGTATCAA AGCCTCTGGC CCACCACATC 
CCTGTGGAGA AGATCTGTGA GAAGCTTAAG AAGAAGGACA GCCAGATATG TGAGCTAAAG 
TATGACAAGC AGATTGACCT GAGCACAGTG GACCTGAAGA AGCTCCGAGT TAAAGAGCTG 
AAGAAGATTC TGGATGACTG GGGGGAGACA TGCAAAGGCT GTGCAGAAAA GTCTGACTAC 
ATCCGGAAGA TAAATGAACT GATGCCTAAA TATGCCCCCA AGGCAGCCAG TGCACGGACC 
GATTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.