NOS3 cDNA ORF clone, Ovis aries(sheep)

The following NOS3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOS3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OOv16957 XM_012135385.2
Latest version!
Ovis aries musimon nitric oxide synthase 3 (NOS3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OOv16958 XM_012135387.2
Latest version!
Ovis aries musimon nitric oxide synthase 3 (NOS3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OOv16959 XM_012135386.2
Latest version!
Ovis aries musimon nitric oxide synthase 3 (NOS3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OSm79687 XM_027969002.1
Latest version!
Ovis aries nitric oxide synthase 3 (NOS3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OSm00217 NM_001129901.1
Latest version!
Ovis aries nitric oxide synthase 3 (NOS3), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OSm49322 XM_015095442.1
Latest version!
Ovis aries nitric oxide synthase 3 (NOS3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OOv16957
    Clone ID Related Accession (Same CDS sequence) XM_012135385.2
    Accession Version XM_012135385.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product nitric oxide synthase, endothelial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942407.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012135385.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAACC TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CCTGTGGCCT GGGGCTGGGG 
    CTGGGTTTTG GGCTATGCGG CAAGCAGGGC CCGGCCTCCC CAGCACCTGA GCCCAGCTGG
    GCCCCCGCAC CTGCCACCCC GCAAGCGCCA GACCACAGCC CAGCTCCCAG CAGCCCCACG
    CTGACCCGGC CTCCGGAGGG GCCCAAGTTC CCTCGCGTGA AGAACTGGGA GCTGGGGAGC
    ATCACCTACG ACACCCTGTG CGCACAGTCC CAGCAGGACG GGCCCTGCAC CCCCAGACGC
    TGCCTCGGCT CCCTGGTGTT GCCCCGGAAA CTGCAGACTC GGCCCTCCCA AGGACCTCCG
    CCCGCTGAGC AGCTGCTGAG CCAGGCCAGG GACTTCATCA ACCAGTACTA CAGCTCCATT
    AAGAGGAGCG GCTCCCAGGC TCATGAGGAG CGGCTTCAGG AGGTGGAGGC CGAGGTGGCA
    TCCACGGGCA CCTACCACCT CCGAGAGAGC GAGCTGGTGT TCGGGGCCAA GCAGGCCTGG
    CGCAACGCGC CCCGCTGCGT GGGCCGCATC CAGTGGGGGA AGCTGCAGGT GTTCGATGCC
    CGGGACTGCA GCTCAGCACA GGAGATGTTC ACCTACATCT GCAACCACAT CAAGTACGCC
    ACCAACCGTG GCAACCTTCG CTCGGCCATC ACAGTGTTCC CGCAGCGCGC CCCGGGCCGC
    GGAGACTTCC GGATCTGGAA CACCCAGCTG GTGCGCTACG CAGGCTACAG ACAGCAGGAC
    GGCTCTGTGC GTGGGGACCC GGCCAACGTG GAGATCACGG AGCTCTGCAT CCAGCACGGC
    TGGTCCCCCG GAAACGGCCG CTTCGACGTG CTGCCCCTGC TGCTCCAGGC CCCAGATGAG
    GCTCCAGAGC TCTTTGTTCT GCCCCCCGAG CTGGTCCTCG AAGTGCCCCT GGAGCACCCC
    ACACTGGAGT GGTTCGCGGC CCTGGGCCTG CGCTGGTATG CCCTCCCGGC CGTGTCCAAC
    ATGCTGCTGG AAATCGGGGG TCTGGAGTTC CCTGCGGCCC CCTTCAGCGG CTGGTACATG
    AGCACGGAGA TTGGCACGCG GAACCTGTGT GACCCTCACC GCTACAACAT CCTGGAGGAT
    GTGGCTGTCT GCATGGACCT CGACACGCGG ACCACCTCGT CCCTGTGGAA GGACAAGGCG
    GCCGTGGAGA TCAACCTGGC TGTGCTGCAC AGCTTTCAGC TCGCCAAGGT GACCATCGTG
    GACCACCACG CCGCCACAGT GTCCTTCATG AAGCACCTGG AAAACGAGCA GAAGGCCCGG
    GGGGGCTGCC CCGCCGACTG GGCCTGGATC GTGCCCCCCA TCTCGGGCAG CCTCACGCCC
    GTCTTCCACC AGGAGATGGT CAATTACGTC CTGTCCCCTG CCTTCCGCTA CCAGCCAGAC
    CCCTGGAAGG GGAGCGCGGC CAAGGGCGCC GGCATCACCA GGAAGAAGAC CTTTAAGGAA
    GTGGCCAACG CAGTGAAGAT CTCTGCCTCA CTCATGGGCA CCCTGATGGC CAAGCGAGTG
    AAAGCAACCA TCCTGTACGC CTCTGAGACC GGCCGGGCCC AGAGCTATGC TCAGCAGCTG
    GGGAGGCTCT TCCGGAAGGC CTTTGACCCC CGGGTCCTGT GCATGGACGA GTATGACGTG
    GTGTCCCTGG AGCACGAGGC GCTGGTGCTG GTGGTGACCA GCACCTTTGG GAATGGCGAT
    CCCCCGGAGA ATGGAGAGAG TTTTGCAGCT GCCCTGATGG AGATGTCGGG GCCCTACAAC
    AGCTCCCCGA GGCCGGAACA GCACAAGAGT TACAAGATCC GCTTCAACAG TGTCTCCTGC
    TCCGACCCGC TGGTGTCCTC CTGGCGGCGG AAGAGAAAGG AGTCCAGCAA CACAGACAGC
    GCAGGGGCCC TGGGGACCCT CAGGTTCTGT GTGTTTGGAC TGGGCTCCCG GGCATATCCC
    CACTTCTGCG CCTTTGCGCG TGCGGTGGAC ACCCGGCTGG AAGAGCTGGG AGGGGAGCGG
    CTGCTGCAGC TGGGCCAGGG CGACGAGCTC TGCGGCCAGG AGGAGGCCTT CCGTGGCTGG
    GCAAAGGCGG CCTTCCAGGC CTCCTGCGAG ACGTTCTGCG TCGGGGAGGA GGCCAAGGCC
    GCCGCCCAGG ACATCTTCAG CCCCAAACGG AGCTGGAAAC GCCAGAGGTA CCGGCTGAGC
    ACCCAGGCCG AGGGCCTCCA GCTGCTGCCA GGCCTGATCC ACGTGCACAG ACGGAAGATG
    TTTCAGGCCA CAGTCCTCTC AGTGGAAAAC CTGCAAAGCA GCAAGTCCAC CCGGGCCACC
    ATCCTGGTGC GCCTGGACAC TGCAGGCCAG GAGGGGCTGC AGTACCAGCC AGGGGACCAC
    ATAGGCATCT GCCCGCCCAA CCGGCCGGGC CTCGTGGAGG CGCTGCTGAG CCGCGTGGAG
    GACCCACCGC CGCCCACCGA GTCTGTGGCT GTGGAGCAGC TGGAGAAGGG CAGCCCAGGC
    GGCCCTCCTC CCAGCTGGGT GCGGGACCCG CGGCTGCCCC CGTGCACGCT GCGCCAGGCT
    CTCACCTTCT TCCTGGACAT CACCTCCCCG CCCAGCCCCC GGCTTCTCCG ACTGCTCAGC
    ACCCTGGCTG AAGAACCCAG CGAACAGCAG GAGCTCGAGA CCCTCAGTCA GGACCCCCGG
    CGCTACGAGG AGTGGAAGTG GTTCCGCTGC CCCACGCTGC TGGAGGTGCT GGAGCAGTTC
    CCGTCCGTGG CACTGCCCGC CCCACTGCTC CTCACCCAGC TGCCCCTGCT GCAGCCCCGG
    TACTACTCCG TCAGCTCGGC CCCCAGCGCC CACCCCGGAG AGGTCCACCT CACGGTGGCT
    GTGCTGGCGT ACAGGACCCA AGATGGGCTG GGCCCCCTAC ACTACGGGGT CTGCTCCACG
    TGGCTGAGCC AACTCAAGAC TGGAGACCCA GTGCCCTGCT TCATCAGGGG GGCTCCCTCT
    TTCCGGCTGC CGCCTGACCC CTACGTGCCC TGCATCCTCG TGGGCCCCGG CACTGGCATC
    GCCCCCTTCC GGGGATTTTG GCAGGAGCGG CTGCACGACA TTGAGAGCAA AGGGCTGCAG
    CCCGCCCCCA TGACCCTGGT GTTCGGCTGC CGGTGCTCCC AACTCGACCA TCTCTACCGC
    GACGAGGTGC AGGACGCCCA GGAGCGCGGG GTGTTTGGCC GCGTCCTCAC CGCCTTCTCC
    CGGGAACCTG ACAGCCCCAA GACCTACGTA CAGGACATCC TGAGAACCGA GCTGGCTGCC
    GAGGTGCACC GCGTGCTGTG CCTCGAGCGG GGCCACATGT TTGTCTGCGG CGACGTCACT
    ATGGCAACCA GCGTCCTGCA GACGGTGCAG CGCATCTTGG CGACAGAGGG CGGCATGGAG
    CTGGACGAGG CGGGCGACGT CATCGGCGTG CTGCGGGATC AGCAACGCTA TCACGAGGAC
    ATTTTTGGCC TCACGCTGCG CACCCAGGAA GTGACAAGCC GCATACGTAC CCAGAGCTTT
    TCCCTGCAGG AGCGGCATCT GCGGGGCGCG GTGCCCTGGG CCTTCGACCC GCCAGGCCCA
    GACACCCCCG GCCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011990775.1
    CDS3..3620
    Translation

    Target ORF information:

    RefSeq Version XM_012135385.2
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon nitric oxide synthase 3 (NOS3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012135385.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAACC TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CCTGTGGCCT GGGGCTGGGG 
    CTGGGTTTTG GGCTATGCGG CAAGCAGGGC CCGGCCTCCC CAGCACCTGA GCCCAGCTGG
    GCCCCCGCAC CTGCCACCCC GCAAGCGCCA GACCACAGCC CAGCTCCCAG CAGCCCCACG
    CTGACCCGGC CTCCGGAGGG GCCCAAGTTC CCTCGCGTGA AGAACTGGGA GCTGGGGAGC
    ATCACCTACG ACACCCTGTG CGCACAGTCC CAGCAGGACG GGCCCTGCAC CCCCAGACGC
    TGCCTCGGCT CCCTGGTGTT GCCCCGGAAA CTGCAGACTC GGCCCTCCCA AGGACCTCCG
    CCCGCTGAGC AGCTGCTGAG CCAGGCCAGG GACTTCATCA ACCAGTACTA CAGCTCCATT
    AAGAGGAGCG GCTCCCAGGC TCATGAGGAG CGGCTTCAGG AGGTGGAGGC CGAGGTGGCA
    TCCACGGGCA CCTACCACCT CCGAGAGAGC GAGCTGGTGT TCGGGGCCAA GCAGGCCTGG
    CGCAACGCGC CCCGCTGCGT GGGCCGCATC CAGTGGGGGA AGCTGCAGGT GTTCGATGCC
    CGGGACTGCA GCTCAGCACA GGAGATGTTC ACCTACATCT GCAACCACAT CAAGTACGCC
    ACCAACCGTG GCAACCTTCG CTCGGCCATC ACAGTGTTCC CGCAGCGCGC CCCGGGCCGC
    GGAGACTTCC GGATCTGGAA CACCCAGCTG GTGCGCTACG CAGGCTACAG ACAGCAGGAC
    GGCTCTGTGC GTGGGGACCC GGCCAACGTG GAGATCACGG AGCTCTGCAT CCAGCACGGC
    TGGTCCCCCG GAAACGGCCG CTTCGACGTG CTGCCCCTGC TGCTCCAGGC CCCAGATGAG
    GCTCCAGAGC TCTTTGTTCT GCCCCCCGAG CTGGTCCTCG AAGTGCCCCT GGAGCACCCC
    ACACTGGAGT GGTTCGCGGC CCTGGGCCTG CGCTGGTATG CCCTCCCGGC CGTGTCCAAC
    ATGCTGCTGG AAATCGGGGG TCTGGAGTTC CCTGCGGCCC CCTTCAGCGG CTGGTACATG
    AGCACGGAGA TTGGCACGCG GAACCTGTGT GACCCTCACC GCTACAACAT CCTGGAGGAT
    GTGGCTGTCT GCATGGACCT CGACACGCGG ACCACCTCGT CCCTGTGGAA GGACAAGGCG
    GCCGTGGAGA TCAACCTGGC TGTGCTGCAC AGCTTTCAGC TCGCCAAGGT GACCATCGTG
    GACCACCACG CCGCCACAGT GTCCTTCATG AAGCACCTGG AAAACGAGCA GAAGGCCCGG
    GGGGGCTGCC CCGCCGACTG GGCCTGGATC GTGCCCCCCA TCTCGGGCAG CCTCACGCCC
    GTCTTCCACC AGGAGATGGT CAATTACGTC CTGTCCCCTG CCTTCCGCTA CCAGCCAGAC
    CCCTGGAAGG GGAGCGCGGC CAAGGGCGCC GGCATCACCA GGAAGAAGAC CTTTAAGGAA
    GTGGCCAACG CAGTGAAGAT CTCTGCCTCA CTCATGGGCA CCCTGATGGC CAAGCGAGTG
    AAAGCAACCA TCCTGTACGC CTCTGAGACC GGCCGGGCCC AGAGCTATGC TCAGCAGCTG
    GGGAGGCTCT TCCGGAAGGC CTTTGACCCC CGGGTCCTGT GCATGGACGA GTATGACGTG
    GTGTCCCTGG AGCACGAGGC GCTGGTGCTG GTGGTGACCA GCACCTTTGG GAATGGCGAT
    CCCCCGGAGA ATGGAGAGAG TTTTGCAGCT GCCCTGATGG AGATGTCGGG GCCCTACAAC
    AGCTCCCCGA GGCCGGAACA GCACAAGAGT TACAAGATCC GCTTCAACAG TGTCTCCTGC
    TCCGACCCGC TGGTGTCCTC CTGGCGGCGG AAGAGAAAGG AGTCCAGCAA CACAGACAGC
    GCAGGGGCCC TGGGGACCCT CAGGTTCTGT GTGTTTGGAC TGGGCTCCCG GGCATATCCC
    CACTTCTGCG CCTTTGCGCG TGCGGTGGAC ACCCGGCTGG AAGAGCTGGG AGGGGAGCGG
    CTGCTGCAGC TGGGCCAGGG CGACGAGCTC TGCGGCCAGG AGGAGGCCTT CCGTGGCTGG
    GCAAAGGCGG CCTTCCAGGC CTCCTGCGAG ACGTTCTGCG TCGGGGAGGA GGCCAAGGCC
    GCCGCCCAGG ACATCTTCAG CCCCAAACGG AGCTGGAAAC GCCAGAGGTA CCGGCTGAGC
    ACCCAGGCCG AGGGCCTCCA GCTGCTGCCA GGCCTGATCC ACGTGCACAG ACGGAAGATG
    TTTCAGGCCA CAGTCCTCTC AGTGGAAAAC CTGCAAAGCA GCAAGTCCAC CCGGGCCACC
    ATCCTGGTGC GCCTGGACAC TGCAGGCCAG GAGGGGCTGC AGTACCAGCC AGGGGACCAC
    ATAGGCATCT GCCCGCCCAA CCGGCCGGGC CTCGTGGAGG CGCTGCTGAG CCGCGTGGAG
    GACCCACCGC CGCCCACCGA GTCTGTGGCT GTGGAGCAGC TGGAGAAGGG CAGCCCAGGC
    GGCCCTCCTC CCAGCTGGGT GCGGGACCCG CGGCTGCCCC CGTGCACGCT GCGCCAGGCT
    CTCACCTTCT TCCTGGACAT CACCTCCCCG CCCAGCCCCC GGCTTCTCCG ACTGCTCAGC
    ACCCTGGCTG AAGAACCCAG CGAACAGCAG GAGCTCGAGA CCCTCAGTCA GGACCCCCGG
    CGCTACGAGG AGTGGAAGTG GTTCCGCTGC CCCACGCTGC TGGAGGTGCT GGAGCAGTTC
    CCGTCCGTGG CACTGCCCGC CCCACTGCTC CTCACCCAGC TGCCCCTGCT GCAGCCCCGG
    TACTACTCCG TCAGCTCGGC CCCCAGCGCC CACCCCGGAG AGGTCCACCT CACGGTGGCT
    GTGCTGGCGT ACAGGACCCA AGATGGGCTG GGCCCCCTAC ACTACGGGGT CTGCTCCACG
    TGGCTGAGCC AACTCAAGAC TGGAGACCCA GTGCCCTGCT TCATCAGGGG GGCTCCCTCT
    TTCCGGCTGC CGCCTGACCC CTACGTGCCC TGCATCCTCG TGGGCCCCGG CACTGGCATC
    GCCCCCTTCC GGGGATTTTG GCAGGAGCGG CTGCACGACA TTGAGAGCAA AGGGCTGCAG
    CCCGCCCCCA TGACCCTGGT GTTCGGCTGC CGGTGCTCCC AACTCGACCA TCTCTACCGC
    GACGAGGTGC AGGACGCCCA GGAGCGCGGG GTGTTTGGCC GCGTCCTCAC CGCCTTCTCC
    CGGGAACCTG ACAGCCCCAA GACCTACGTA CAGGACATCC TGAGAACCGA GCTGGCTGCC
    GAGGTGCACC GCGTGCTGTG CCTCGAGCGG GGCCACATGT TTGTCTGCGG CGACGTCACT
    ATGGCAACCA GCGTCCTGCA GACGGTGCAG CGCATCTTGG CGACAGAGGG CGGCATGGAG
    CTGGACGAGG CGGGCGACGT CATCGGCGTG CTGCGGGATC AGCAACGCTA TCACGAGGAC
    ATTTTTGGCC TCACGCTGCG CACCCAGGAA GTGACAAGCC GCATACGTAC CCAGAGCTTT
    TCCCTGCAGG AGCGGCATCT GCGGGGCGCG GTGCCCTGGG CCTTCGACCC GCCAGGCCCA
    GACACCCCCG GCCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv16958
    Clone ID Related Accession (Same CDS sequence) XM_012135387.2
    Accession Version XM_012135387.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3258bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product nitric oxide synthase, endothelial isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942407.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012135387.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGCAGGA TCCCCGGAGG CCACCTGATG CACCCATCCC CACCTCCCCA TCATGTGTGC 
    CCAAGGAGGG TCAGAAGATG GGCCGGGCTC ACCCGCCTTT GCTTGAGCAC CGTGAGAAAC
    AGGGAACTCT CGACATCCAC ACCCCGGGTG GCCTTCCAGT CACTTGGTGG CCTTTCCCCA
    GTGATCAGTT TGGCAGTGAC TGGGTCCCTC CTCTGTACCA GAGACCAGGT GTTCGATGCC
    CGGGACTGCA GCTCAGCACA GGAGATGTTC ACCTACATCT GCAACCACAT CAAGTACGCC
    ACCAACCGTG GCAACCTTCG CTCGGCCATC ACAGTGTTCC CGCAGCGCGC CCCGGGCCGC
    GGAGACTTCC GGATCTGGAA CACCCAGCTG GTGCGCTACG CAGGCTACAG ACAGCAGGAC
    GGCTCTGTGC GTGGGGACCC GGCCAACGTG GAGATCACGG AGCTCTGCAT CCAGCACGGC
    TGGTCCCCCG GAAACGGCCG CTTCGACGTG CTGCCCCTGC TGCTCCAGGC CCCAGATGAG
    GCTCCAGAGC TCTTTGTTCT GCCCCCCGAG CTGGTCCTCG AAGTGCCCCT GGAGCACCCC
    ACACTGGAGT GGTTCGCGGC CCTGGGCCTG CGCTGGTATG CCCTCCCGGC CGTGTCCAAC
    ATGCTGCTGG AAATCGGGGG TCTGGAGTTC CCTGCGGCCC CCTTCAGCGG CTGGTACATG
    AGCACGGAGA TTGGCACGCG GAACCTGTGT GACCCTCACC GCTACAACAT CCTGGAGGAT
    GTGGCTGTCT GCATGGACCT CGACACGCGG ACCACCTCGT CCCTGTGGAA GGACAAGGCG
    GCCGTGGAGA TCAACCTGGC TGTGCTGCAC AGCTTTCAGC TCGCCAAGGT GACCATCGTG
    GACCACCACG CCGCCACAGT GTCCTTCATG AAGCACCTGG AAAACGAGCA GAAGGCCCGG
    GGGGGCTGCC CCGCCGACTG GGCCTGGATC GTGCCCCCCA TCTCGGGCAG CCTCACGCCC
    GTCTTCCACC AGGAGATGGT CAATTACGTC CTGTCCCCTG CCTTCCGCTA CCAGCCAGAC
    CCCTGGAAGG GGAGCGCGGC CAAGGGCGCC GGCATCACCA GGAAGAAGAC CTTTAAGGAA
    GTGGCCAACG CAGTGAAGAT CTCTGCCTCA CTCATGGGCA CCCTGATGGC CAAGCGAGTG
    AAAGCAACCA TCCTGTACGC CTCTGAGACC GGCCGGGCCC AGAGCTATGC TCAGCAGCTG
    GGGAGGCTCT TCCGGAAGGC CTTTGACCCC CGGGTCCTGT GCATGGACGA GTATGACGTG
    GTGTCCCTGG AGCACGAGGC GCTGGTGCTG GTGGTGACCA GCACCTTTGG GAATGGCGAT
    CCCCCGGAGA ATGGAGAGAG TTTTGCAGCT GCCCTGATGG AGATGTCGGG GCCCTACAAC
    AGCTCCCCGA GGCCGGAACA GCACAAGAGT TACAAGATCC GCTTCAACAG TGTCTCCTGC
    TCCGACCCGC TGGTGTCCTC CTGGCGGCGG AAGAGAAAGG AGTCCAGCAA CACAGACAGC
    GCAGGGGCCC TGGGGACCCT CAGGTTCTGT GTGTTTGGAC TGGGCTCCCG GGCATATCCC
    CACTTCTGCG CCTTTGCGCG TGCGGTGGAC ACCCGGCTGG AAGAGCTGGG AGGGGAGCGG
    CTGCTGCAGC TGGGCCAGGG CGACGAGCTC TGCGGCCAGG AGGAGGCCTT CCGTGGCTGG
    GCAAAGGCGG CCTTCCAGGC CTCCTGCGAG ACGTTCTGCG TCGGGGAGGA GGCCAAGGCC
    GCCGCCCAGG ACATCTTCAG CCCCAAACGG AGCTGGAAAC GCCAGAGGTA CCGGCTGAGC
    ACCCAGGCCG AGGGCCTCCA GCTGCTGCCA GGCCTGATCC ACGTGCACAG ACGGAAGATG
    TTTCAGGCCA CAGTCCTCTC AGTGGAAAAC CTGCAAAGCA GCAAGTCCAC CCGGGCCACC
    ATCCTGGTGC GCCTGGACAC TGCAGGCCAG GAGGGGCTGC AGTACCAGCC AGGGGACCAC
    ATAGGCATCT GCCCGCCCAA CCGGCCGGGC CTCGTGGAGG CGCTGCTGAG CCGCGTGGAG
    GACCCACCGC CGCCCACCGA GTCTGTGGCT GTGGAGCAGC TGGAGAAGGG CAGCCCAGGC
    GGCCCTCCTC CCAGCTGGGT GCGGGACCCG CGGCTGCCCC CGTGCACGCT GCGCCAGGCT
    CTCACCTTCT TCCTGGACAT CACCTCCCCG CCCAGCCCCC GGCTTCTCCG ACTGCTCAGC
    ACCCTGGCTG AAGAACCCAG CGAACAGCAG GAGCTCGAGA CCCTCAGTCA GGACCCCCGG
    CGCTACGAGG AGTGGAAGTG GTTCCGCTGC CCCACGCTGC TGGAGGTGCT GGAGCAGTTC
    CCGTCCGTGG CACTGCCCGC CCCACTGCTC CTCACCCAGC TGCCCCTGCT GCAGCCCCGG
    TACTACTCCG TCAGCTCGGC CCCCAGCGCC CACCCCGGAG AGGTCCACCT CACGGTGGCT
    GTGCTGGCGT ACAGGACCCA AGATGGGCTG GGCCCCCTAC ACTACGGGGT CTGCTCCACG
    TGGCTGAGCC AACTCAAGAC TGGAGACCCA GTGCCCTGCT TCATCAGGGG GGCTCCCTCT
    TTCCGGCTGC CGCCTGACCC CTACGTGCCC TGCATCCTCG TGGGCCCCGG CACTGGCATC
    GCCCCCTTCC GGGGATTTTG GCAGGAGCGG CTGCACGACA TTGAGAGCAA AGGGCTGCAG
    CCCGCCCCCA TGACCCTGGT GTTCGGCTGC CGGTGCTCCC AACTCGACCA TCTCTACCGC
    GACGAGGTGC AGGACGCCCA GGAGCGCGGG GTGTTTGGCC GCGTCCTCAC CGCCTTCTCC
    CGGGAACCTG ACAGCCCCAA GACCTACGTA CAGGACATCC TGAGAACCGA GCTGGCTGCC
    GAGGTGCACC GCGTGCTGTG CCTCGAGCGG GGCCACATGT TTGTCTGCGG CGACGTCACT
    ATGGCAACCA GCGTCCTGCA GACGGTGCAG CGCATCTTGG CGACAGAGGG CGGCATGGAG
    CTGGACGAGG CGGGCGACGT CATCGGCGTG CTGCGGGATC AGCAACGCTA TCACGAGGAC
    ATTTTTGGCC TCACGCTGCG CACCCAGGAA GTGACAAGCC GCATACGTAC CCAGAGCTTT
    TCCCTGCAGG AGCGGCATCT GCGGGGCGCG GTGCCCTGGG CCTTCGACCC GCCAGGCCCA
    GACACCCCCG GCCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011990777.1
    CDS233..3490
    Translation

    Target ORF information:

    RefSeq Version XM_012135387.2
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon nitric oxide synthase 3 (NOS3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012135387.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGGCAGGA TCCCCGGAGG CCACCTGATG CACCCATCCC CACCTCCCCA TCATGTGTGC 
    CCAAGGAGGG TCAGAAGATG GGCCGGGCTC ACCCGCCTTT GCTTGAGCAC CGTGAGAAAC
    AGGGAACTCT CGACATCCAC ACCCCGGGTG GCCTTCCAGT CACTTGGTGG CCTTTCCCCA
    GTGATCAGTT TGGCAGTGAC TGGGTCCCTC CTCTGTACCA GAGACCAGGT GTTCGATGCC
    CGGGACTGCA GCTCAGCACA GGAGATGTTC ACCTACATCT GCAACCACAT CAAGTACGCC
    ACCAACCGTG GCAACCTTCG CTCGGCCATC ACAGTGTTCC CGCAGCGCGC CCCGGGCCGC
    GGAGACTTCC GGATCTGGAA CACCCAGCTG GTGCGCTACG CAGGCTACAG ACAGCAGGAC
    GGCTCTGTGC GTGGGGACCC GGCCAACGTG GAGATCACGG AGCTCTGCAT CCAGCACGGC
    TGGTCCCCCG GAAACGGCCG CTTCGACGTG CTGCCCCTGC TGCTCCAGGC CCCAGATGAG
    GCTCCAGAGC TCTTTGTTCT GCCCCCCGAG CTGGTCCTCG AAGTGCCCCT GGAGCACCCC
    ACACTGGAGT GGTTCGCGGC CCTGGGCCTG CGCTGGTATG CCCTCCCGGC CGTGTCCAAC
    ATGCTGCTGG AAATCGGGGG TCTGGAGTTC CCTGCGGCCC CCTTCAGCGG CTGGTACATG
    AGCACGGAGA TTGGCACGCG GAACCTGTGT GACCCTCACC GCTACAACAT CCTGGAGGAT
    GTGGCTGTCT GCATGGACCT CGACACGCGG ACCACCTCGT CCCTGTGGAA GGACAAGGCG
    GCCGTGGAGA TCAACCTGGC TGTGCTGCAC AGCTTTCAGC TCGCCAAGGT GACCATCGTG
    GACCACCACG CCGCCACAGT GTCCTTCATG AAGCACCTGG AAAACGAGCA GAAGGCCCGG
    GGGGGCTGCC CCGCCGACTG GGCCTGGATC GTGCCCCCCA TCTCGGGCAG CCTCACGCCC
    GTCTTCCACC AGGAGATGGT CAATTACGTC CTGTCCCCTG CCTTCCGCTA CCAGCCAGAC
    CCCTGGAAGG GGAGCGCGGC CAAGGGCGCC GGCATCACCA GGAAGAAGAC CTTTAAGGAA
    GTGGCCAACG CAGTGAAGAT CTCTGCCTCA CTCATGGGCA CCCTGATGGC CAAGCGAGTG
    AAAGCAACCA TCCTGTACGC CTCTGAGACC GGCCGGGCCC AGAGCTATGC TCAGCAGCTG
    GGGAGGCTCT TCCGGAAGGC CTTTGACCCC CGGGTCCTGT GCATGGACGA GTATGACGTG
    GTGTCCCTGG AGCACGAGGC GCTGGTGCTG GTGGTGACCA GCACCTTTGG GAATGGCGAT
    CCCCCGGAGA ATGGAGAGAG TTTTGCAGCT GCCCTGATGG AGATGTCGGG GCCCTACAAC
    AGCTCCCCGA GGCCGGAACA GCACAAGAGT TACAAGATCC GCTTCAACAG TGTCTCCTGC
    TCCGACCCGC TGGTGTCCTC CTGGCGGCGG AAGAGAAAGG AGTCCAGCAA CACAGACAGC
    GCAGGGGCCC TGGGGACCCT CAGGTTCTGT GTGTTTGGAC TGGGCTCCCG GGCATATCCC
    CACTTCTGCG CCTTTGCGCG TGCGGTGGAC ACCCGGCTGG AAGAGCTGGG AGGGGAGCGG
    CTGCTGCAGC TGGGCCAGGG CGACGAGCTC TGCGGCCAGG AGGAGGCCTT CCGTGGCTGG
    GCAAAGGCGG CCTTCCAGGC CTCCTGCGAG ACGTTCTGCG TCGGGGAGGA GGCCAAGGCC
    GCCGCCCAGG ACATCTTCAG CCCCAAACGG AGCTGGAAAC GCCAGAGGTA CCGGCTGAGC
    ACCCAGGCCG AGGGCCTCCA GCTGCTGCCA GGCCTGATCC ACGTGCACAG ACGGAAGATG
    TTTCAGGCCA CAGTCCTCTC AGTGGAAAAC CTGCAAAGCA GCAAGTCCAC CCGGGCCACC
    ATCCTGGTGC GCCTGGACAC TGCAGGCCAG GAGGGGCTGC AGTACCAGCC AGGGGACCAC
    ATAGGCATCT GCCCGCCCAA CCGGCCGGGC CTCGTGGAGG CGCTGCTGAG CCGCGTGGAG
    GACCCACCGC CGCCCACCGA GTCTGTGGCT GTGGAGCAGC TGGAGAAGGG CAGCCCAGGC
    GGCCCTCCTC CCAGCTGGGT GCGGGACCCG CGGCTGCCCC CGTGCACGCT GCGCCAGGCT
    CTCACCTTCT TCCTGGACAT CACCTCCCCG CCCAGCCCCC GGCTTCTCCG ACTGCTCAGC
    ACCCTGGCTG AAGAACCCAG CGAACAGCAG GAGCTCGAGA CCCTCAGTCA GGACCCCCGG
    CGCTACGAGG AGTGGAAGTG GTTCCGCTGC CCCACGCTGC TGGAGGTGCT GGAGCAGTTC
    CCGTCCGTGG CACTGCCCGC CCCACTGCTC CTCACCCAGC TGCCCCTGCT GCAGCCCCGG
    TACTACTCCG TCAGCTCGGC CCCCAGCGCC CACCCCGGAG AGGTCCACCT CACGGTGGCT
    GTGCTGGCGT ACAGGACCCA AGATGGGCTG GGCCCCCTAC ACTACGGGGT CTGCTCCACG
    TGGCTGAGCC AACTCAAGAC TGGAGACCCA GTGCCCTGCT TCATCAGGGG GGCTCCCTCT
    TTCCGGCTGC CGCCTGACCC CTACGTGCCC TGCATCCTCG TGGGCCCCGG CACTGGCATC
    GCCCCCTTCC GGGGATTTTG GCAGGAGCGG CTGCACGACA TTGAGAGCAA AGGGCTGCAG
    CCCGCCCCCA TGACCCTGGT GTTCGGCTGC CGGTGCTCCC AACTCGACCA TCTCTACCGC
    GACGAGGTGC AGGACGCCCA GGAGCGCGGG GTGTTTGGCC GCGTCCTCAC CGCCTTCTCC
    CGGGAACCTG ACAGCCCCAA GACCTACGTA CAGGACATCC TGAGAACCGA GCTGGCTGCC
    GAGGTGCACC GCGTGCTGTG CCTCGAGCGG GGCCACATGT TTGTCTGCGG CGACGTCACT
    ATGGCAACCA GCGTCCTGCA GACGGTGCAG CGCATCTTGG CGACAGAGGG CGGCATGGAG
    CTGGACGAGG CGGGCGACGT CATCGGCGTG CTGCGGGATC AGCAACGCTA TCACGAGGAC
    ATTTTTGGCC TCACGCTGCG CACCCAGGAA GTGACAAGCC GCATACGTAC CCAGAGCTTT
    TCCCTGCAGG AGCGGCATCT GCGGGGCGCG GTGCCCTGGG CCTTCGACCC GCCAGGCCCA
    GACACCCCCG GCCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv16959
    Clone ID Related Accession (Same CDS sequence) XM_012135386.2
    Accession Version XM_012135386.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3060bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product nitric oxide synthase, endothelial isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942407.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012135386.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGGCAACC TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CCTGTGGCCT GGGGCTGGGG 
    CTGGGTTTTG GGCTATGCGG CAAGCAGGGC CCGGCCTCCC CAGCACCTGA GCCCAGCTGG
    GCCCCCGCAC CTGCCACCCC GCAAGCGCCA GACCACAGCC CAGCTCCCAG CAGCCCCACG
    CTGACCCGGC CTCCGGAGGG GCCCAAGTTC CCTCGCGTGA AGAACTGGGA GCTGGGGAGC
    ATCACCTACG ACACCCTGTG CGCACAGTCC CAGCAGGACG GGCCCTGCAC CCCCAGACGC
    TGCCTCGGCT CCCTGGTGTT GCCCCGGAAA CTGCAGACTC GGCCCTCCCA AGGACCTCCG
    CCCGCTGAGC AGCTGCTGAG CCAGGCCAGG GACTTCATCA ACCAGTACTA CAGCTCCATT
    AAGAGGAGCG GCTCCCAGGC TCATGAGGAG CGGCTTCAGG AGGTGGAGGC CGAGGTGGCA
    TCCACGGGCA CCTACCACCT CCGAGAGAGC GAGCTGGTGT TCGGGGCCAA GCAGGCCTGG
    CGCAACGCGC CCCGCTGCGT GGGCCGCATC CAGTGGGGGA AGCTGCAGGT GTTCGATGCC
    CGGGACTGCA GCTCAGCACA GGAGATGTTC ACCTACATCT GCAACCACAT CAAGTACGCC
    ACCAACCGTG GCAACCTTCG CTCGGCCATC ACAGTGTTCC CGCAGCGCGC CCCGGGCCGC
    GGAGACTTCC GGATCTGGAA CACCCAGCTG GTGCGCTACG CAGGCTACAG ACAGCAGGAC
    GGCTCTGTGC GTGGGGACCC GGCCAACGTG GAGATCACGG AGCTCTGCAT CCAGCACGGC
    TGGTCCCCCG GAAACGGCCG CTTCGACGTG CTGCCCCTGC TGCTCCAGGC CCCAGATGAG
    GCTCCAGAGC TCTTTGTTCT GCCCCCCGAG CTGGTCCTCG AAGTGCCCCT GGAGCACCCC
    ACACTGGAGT GGTTCGCGGC CCTGGGCCTG CGCTGGTATG CCCTCCCGGC CGTGTCCAAC
    ATGCTGCTGG AAATCGGGGG TCTGGAGTTC CCTGCGGCCC CCTTCAGCGG CTGGTACATG
    AGCACGGAGA TTGGCACGCG GAACCTGTGT GACCCTCACC GCTACAACAT CCTGGAGGAT
    GTGGCTGTCT GCATGGACCT CGACACGCGG ACCACCTCGT CCCTGTGGAA GGACAAGGCG
    GCCGTGGAGA TCAACCTGGC TGTGCTGCAC AGCTTTCAGC TCGCCAAGGT GACCATCGTG
    GACCACCACG CCGCCACAGT GTCCTTCATG AAGCACCTGG AAAACGAGCA GAAGGCCCGG
    GGGGGCTGCC CCGCCGACTG GGCCTGGATC GTGCCCCCCA TCTCGGGCAG CCTCACGCCC
    GTCTTCCACC AGGAGATGGT CAATTACGTC CTGTCCCCTG CCTTCCGCTA CCAGCCAGAC
    CCCTGGAAGG GGAGCGCGGC CAAGGGCGCC GGCATCACCA GGAAGAAGAC CTTTAAGGAA
    GTGGCCAACG CAGTGAAGAT CTCTGCCTCA CTCATGGGCA CCCTGATGGC CAAGCGAGTG
    AAAGCAACCA TCCTGTACGC CTCTGAGACC GGCCGGGCCC AGAGCTATGC TCAGCAGCTG
    GGGAGGCTCT TCCGGAAGGC CTTTGACCCC CGGGTCCTGT GCATGGACGA GTATGACGTG
    GTGTCCCTGG AGCACGAGGC GCTGGTGCTG GTGGTGACCA GCACCTTTGG GAATGGCGAT
    CCCCCGGAGA ATGGAGAGAG TTTTGCAGCT GCCCTGATGG AGATGTCGGG GCCCTACAAC
    AGCTCCCCGA GGCCGGAACA GCACAAGAGT TACAAGATCC GCTTCAACAG TGTCTCCTGC
    TCCGACCCGC TGGTGTCCTC CTGGCGGCGG AAGAGAAAGG AGTCCAGCAA CACAGACAGC
    GCAGGGGCCC TGGGGACCCT CAGGTTCTGT GTGTTTGGAC TGGGCTCCCG GGCATATCCC
    CACTTCTGCG CCTTTGCGCG TGCGGTGGAC ACCCGGCTGG AAGAGCTGGG AGGGGAGCGG
    CTGCTGCAGC TGGGCCAGGG CGACGAGCTC TGCGGCCAGG AGGAGGCCTT CCGTGGCTGG
    GCAAAGGCGG CCTTCCAGGC CTCCTGCGAG ACGTTCTGCG TCGGGGAGGA GGCCAAGGCC
    GCCGCCCAGG ACATCTTCAG CCCCAAACGG AGCTGGAAAC GCCAGAGGTA CCGGCTGAGC
    ACCCAGGCCG AGGGCCTCCA GCTGCTGCCA GGCCTGATCC ACGTGCACAG ACGGAAGATG
    TTTCAGGCCA CAGTCCTCTC AGTGGAAAAC CTGCAAAGCA GCAAGTCCAC CCGGGCCACC
    ATCCTGGTGC GCCTGGACAC TGCAGGCCAG GAGGGGCTGC AGTACCAGCC AGGGGACCAC
    ATAGGCATCT GCCCGCCCAA CCGGCCGGGC CTCGTGGAGG CGCTGCTGAG CCGCGTGGAG
    GACCCACCGC CGCCCACCGA GTCTGTGGCT GTGGAGCAGC TGGAGAAGGG CAGCCCAGGC
    GGCCCTCCTC CCAGCTGGGT GCGGGACCCG CGGCTGCCCC CGTGCACGCT GCGCCAGGCT
    CTCACCTTCT TCCTGGACAT CACCTCCCCG CCCAGCCCCC GGCTTCTCCG ACTGCTCAGC
    ACCCTGGCTG AAGAACCCAG CGAACAGCAG GAGCTCGAGA CCCTCAGTCA GGACCCCCGG
    CGCTACGAGG AGTGGAAGTG GTTCCGCTGC CCCACGCTGC TGGAGGTGCT GGAGCAGTTC
    CCGTCCGTGG CACTGCCCGC CCCACTGCTC CTCACCCAGC TGCCCCTGCT GCAGCCCCGG
    TACTACTCCG TCAGCTCGGC CCCCAGCGCC CACCCCGGAG AGGTCCACCT CACGGTGGCT
    GTGCTGGCGT ACAGGACCCA AGATGGGCTG GGCCCCCTAC ACTACGGGGT CTGCTCCACG
    TGGCTGAGCC AACTCAAGAC TGGAGACCCA GTGCCCTGCT TCATCAGGGG GTCCTACCTT
    GAAGCTGCAG CTCCCAGGAA CGGGCTGGGA TCCCACCCCA GCAGGCCCTG TCCCTTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011990776.1
    CDS72..3131
    Translation

    Target ORF information:

    RefSeq Version XM_012135386.2
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon nitric oxide synthase 3 (NOS3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012135386.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGGCAACC TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CCTGTGGCCT GGGGCTGGGG 
    CTGGGTTTTG GGCTATGCGG CAAGCAGGGC CCGGCCTCCC CAGCACCTGA GCCCAGCTGG
    GCCCCCGCAC CTGCCACCCC GCAAGCGCCA GACCACAGCC CAGCTCCCAG CAGCCCCACG
    CTGACCCGGC CTCCGGAGGG GCCCAAGTTC CCTCGCGTGA AGAACTGGGA GCTGGGGAGC
    ATCACCTACG ACACCCTGTG CGCACAGTCC CAGCAGGACG GGCCCTGCAC CCCCAGACGC
    TGCCTCGGCT CCCTGGTGTT GCCCCGGAAA CTGCAGACTC GGCCCTCCCA AGGACCTCCG
    CCCGCTGAGC AGCTGCTGAG CCAGGCCAGG GACTTCATCA ACCAGTACTA CAGCTCCATT
    AAGAGGAGCG GCTCCCAGGC TCATGAGGAG CGGCTTCAGG AGGTGGAGGC CGAGGTGGCA
    TCCACGGGCA CCTACCACCT CCGAGAGAGC GAGCTGGTGT TCGGGGCCAA GCAGGCCTGG
    CGCAACGCGC CCCGCTGCGT GGGCCGCATC CAGTGGGGGA AGCTGCAGGT GTTCGATGCC
    CGGGACTGCA GCTCAGCACA GGAGATGTTC ACCTACATCT GCAACCACAT CAAGTACGCC
    ACCAACCGTG GCAACCTTCG CTCGGCCATC ACAGTGTTCC CGCAGCGCGC CCCGGGCCGC
    GGAGACTTCC GGATCTGGAA CACCCAGCTG GTGCGCTACG CAGGCTACAG ACAGCAGGAC
    GGCTCTGTGC GTGGGGACCC GGCCAACGTG GAGATCACGG AGCTCTGCAT CCAGCACGGC
    TGGTCCCCCG GAAACGGCCG CTTCGACGTG CTGCCCCTGC TGCTCCAGGC CCCAGATGAG
    GCTCCAGAGC TCTTTGTTCT GCCCCCCGAG CTGGTCCTCG AAGTGCCCCT GGAGCACCCC
    ACACTGGAGT GGTTCGCGGC CCTGGGCCTG CGCTGGTATG CCCTCCCGGC CGTGTCCAAC
    ATGCTGCTGG AAATCGGGGG TCTGGAGTTC CCTGCGGCCC CCTTCAGCGG CTGGTACATG
    AGCACGGAGA TTGGCACGCG GAACCTGTGT GACCCTCACC GCTACAACAT CCTGGAGGAT
    GTGGCTGTCT GCATGGACCT CGACACGCGG ACCACCTCGT CCCTGTGGAA GGACAAGGCG
    GCCGTGGAGA TCAACCTGGC TGTGCTGCAC AGCTTTCAGC TCGCCAAGGT GACCATCGTG
    GACCACCACG CCGCCACAGT GTCCTTCATG AAGCACCTGG AAAACGAGCA GAAGGCCCGG
    GGGGGCTGCC CCGCCGACTG GGCCTGGATC GTGCCCCCCA TCTCGGGCAG CCTCACGCCC
    GTCTTCCACC AGGAGATGGT CAATTACGTC CTGTCCCCTG CCTTCCGCTA CCAGCCAGAC
    CCCTGGAAGG GGAGCGCGGC CAAGGGCGCC GGCATCACCA GGAAGAAGAC CTTTAAGGAA
    GTGGCCAACG CAGTGAAGAT CTCTGCCTCA CTCATGGGCA CCCTGATGGC CAAGCGAGTG
    AAAGCAACCA TCCTGTACGC CTCTGAGACC GGCCGGGCCC AGAGCTATGC TCAGCAGCTG
    GGGAGGCTCT TCCGGAAGGC CTTTGACCCC CGGGTCCTGT GCATGGACGA GTATGACGTG
    GTGTCCCTGG AGCACGAGGC GCTGGTGCTG GTGGTGACCA GCACCTTTGG GAATGGCGAT
    CCCCCGGAGA ATGGAGAGAG TTTTGCAGCT GCCCTGATGG AGATGTCGGG GCCCTACAAC
    AGCTCCCCGA GGCCGGAACA GCACAAGAGT TACAAGATCC GCTTCAACAG TGTCTCCTGC
    TCCGACCCGC TGGTGTCCTC CTGGCGGCGG AAGAGAAAGG AGTCCAGCAA CACAGACAGC
    GCAGGGGCCC TGGGGACCCT CAGGTTCTGT GTGTTTGGAC TGGGCTCCCG GGCATATCCC
    CACTTCTGCG CCTTTGCGCG TGCGGTGGAC ACCCGGCTGG AAGAGCTGGG AGGGGAGCGG
    CTGCTGCAGC TGGGCCAGGG CGACGAGCTC TGCGGCCAGG AGGAGGCCTT CCGTGGCTGG
    GCAAAGGCGG CCTTCCAGGC CTCCTGCGAG ACGTTCTGCG TCGGGGAGGA GGCCAAGGCC
    GCCGCCCAGG ACATCTTCAG CCCCAAACGG AGCTGGAAAC GCCAGAGGTA CCGGCTGAGC
    ACCCAGGCCG AGGGCCTCCA GCTGCTGCCA GGCCTGATCC ACGTGCACAG ACGGAAGATG
    TTTCAGGCCA CAGTCCTCTC AGTGGAAAAC CTGCAAAGCA GCAAGTCCAC CCGGGCCACC
    ATCCTGGTGC GCCTGGACAC TGCAGGCCAG GAGGGGCTGC AGTACCAGCC AGGGGACCAC
    ATAGGCATCT GCCCGCCCAA CCGGCCGGGC CTCGTGGAGG CGCTGCTGAG CCGCGTGGAG
    GACCCACCGC CGCCCACCGA GTCTGTGGCT GTGGAGCAGC TGGAGAAGGG CAGCCCAGGC
    GGCCCTCCTC CCAGCTGGGT GCGGGACCCG CGGCTGCCCC CGTGCACGCT GCGCCAGGCT
    CTCACCTTCT TCCTGGACAT CACCTCCCCG CCCAGCCCCC GGCTTCTCCG ACTGCTCAGC
    ACCCTGGCTG AAGAACCCAG CGAACAGCAG GAGCTCGAGA CCCTCAGTCA GGACCCCCGG
    CGCTACGAGG AGTGGAAGTG GTTCCGCTGC CCCACGCTGC TGGAGGTGCT GGAGCAGTTC
    CCGTCCGTGG CACTGCCCGC CCCACTGCTC CTCACCCAGC TGCCCCTGCT GCAGCCCCGG
    TACTACTCCG TCAGCTCGGC CCCCAGCGCC CACCCCGGAG AGGTCCACCT CACGGTGGCT
    GTGCTGGCGT ACAGGACCCA AGATGGGCTG GGCCCCCTAC ACTACGGGGT CTGCTCCACG
    TGGCTGAGCC AACTCAAGAC TGGAGACCCA GTGCCCTGCT TCATCAGGGG GTCCTACCTT
    GAAGCTGCAG CTCCCAGGAA CGGGCTGGGA TCCCACCCCA GCAGGCCCTG TCCCTTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm79687
    Clone ID Related Accession (Same CDS sequence) XM_027969002.1
    Accession Version XM_027969002.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-05
    Organism Ovis aries(sheep)
    Product LOW QUALITY PROTEIN: nitric oxide synthase, endothelial
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040255.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAACC TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CCTGTGGCCT GGGGCTGGGG 
    CTGGGTTTTG GGCTATGCGG CAAGCAGGGC CCGGCCTCCC CAGCACCTGA GCCCAGCTGG
    GCCCCCGCAC CTGCCACCCC GCAAGCGCCA GACCACAGCC CAGCTCCCAG CAGCCCCACG
    CTGACCCGGC CTCCGGAGGG GCCCAAGTTC CCTCGCGTGA AGAACTGGGA GCTGGGGAGC
    ATCACCTACG ACACCCTGTG CGCACAGTCC CAGCAGGACG GGCCCTGCAC CCCCAGACGC
    TGCCTCGGCT CCCTGGTGTT GCCCCGGAAA CTGCAGACTC GGCCCTCCCA AGGACCTCCG
    CCCGCTGAGC AGCTGCTGAG CCAGGCCAGG GACTTCATCA ACCAGTACTA CAGCTCCATT
    AAGAGGAGCG GCTCCCAGGC TCATGAGGAG CGGCTTCAGG AGGTGGAGGC CGAGGTGGCA
    TCCACGGGCA CCTACCACCT CCGAGAGAGC GAGCTGGTGT TCGGGGCCAA GCAGGCCTGG
    CGCAACGCGC CCCGCTGCGT GGGCCGCATC CAGTGGGGGA AGCTGCAGGT GTTCGATGCC
    CGGGACTGCA GCTCAGCACA GGAGATGTTC ACCTACATCT GCAACCACAT CAAGTACGCC
    ACCAACCGTG GCAACCTTCG CTCGGCCATC ACAGTGTTCC CGCAGCGCGC CCCGGGCCGC
    GGAGACTTCC GGATCTGGAA CACCCAGCTG GTGCGCTACG CAGGCTACAG ACAGCAGGAC
    GGCTCTGTGC GTGGGGACCC GGCCAACGTG GAGATCACGG AGCTCTGCAT CCAGCACGGC
    TGGTCCCCCG GAAACGGCCG CTTCGACGTG CTGCCCCTGC TGCTCCAGGC CCCAGATGAG
    GCTCCAGAGC TCTTTGTTCT GCCCCCCGAG CTGGTCCTCG AAGTGCCCCT GGAGCACCCC
    ACACTGGAGT GGTTCGCGGC CCTGGGCCTG CGCTGGTATG CCCTCCCGGC CGTGTCCAAC
    ATGCTGCTGG AAATCGGGGG TCTGGAGTTC CCTGCGGCCC CCTTCAGCGG CTGGTACATG
    AGCACGGAGA TTGGCACACG GAACCTGTGT GACCCTCACC GCTACAACAT CCTGGAGGAT
    GTGGCTGTCT GCATGGACCT CGACACGCGG ACCACCTCGT CCCTGTGGAA GGACAAGGCG
    GCCGTGGAGA TCAACCTGGC TGTGCTGCAC AGCTTTCAGC TCGCCAAGGT GACCATCGTG
    GACCACCACG CCGCCACAGT GTCCTTCATG AAGCACCTGG AAAACGAGCA GAAGGCCCGG
    GGGGGCTGCC CCGCCGACTG GGCCTGGATC GTGCCCCCCA TCTCGGGCAG CCTCACGCCC
    GTCTTCCACC AGGAGATGGT CAATTACGTC CTGTCCCCTG CCTTCCGCTA CCAGCCAGAC
    CCCTGGAAGG GGAGCGCGGC CAAGGGCGCC GGCATCACCA GGAAGAAGAC CTTTAAGGAA
    GTGGCCAACG CGGTGAAGAT CTCTGCCTCA CTCATGGGCA CCCTGATGGC CAAGCGAGTG
    AAAGCAACCA TCCTGTACGC CTCTGAGACC GGCCGGGCCC AGAGCTATGC TCAGCAGCTG
    GGGAGGCTCT TCCGGAAGGC CTTTGACCCC CGGGTCCTGT GCATGGACGA GTATGACGTG
    GTGTCCCTGG AGCACGAGGC GCTGGTGCTG GTGGTGACCA GCACCTTTGG GAATGGCGAT
    CCCCCGGAGA ATGGAGAGAG TTTTGCAGCT GCCCTGATGG AGATGTCGGG GCCCTACAAC
    AGCTCCCCGA GGCCGGAACA GCACAAGAGT TACAAGATCC GCTTCAACAG TGTCTCCTGC
    TCCGACCCGC TGGTGTCCTC CTGGCGGCGG AAGAGAAAGG AGTCCAGCAA CACAGACAGC
    GCAGGAGCCC TGGGGACCCT CAGGTTCTGT GTGTTTGGAC TGGGCTCCCG GGCGTATCCC
    CACTTCTGCG CCTTCGCGCG TGCGGTGGAC ACCCGGCTGG AAGAGCTGGG AGGGGAGCGG
    CTGCTGCAGC TGGGCCAGGG CGACGAGCTC TGCGGCCAGG AGGAGGCCTT CCGTGGCTGG
    GCAAAGGCGG CCTTCCAGGC CTCCTGCGAG ACGTTCTGCG TCGGGGAGGA GGCCAAGGCC
    GCCGCCCAGG ACATCTTCAG CCCCAAACGG AGCTGGAAAC GCCAGAGGTA CCGGCTGAGC
    ACCCAGGCCG AGGGCCTCCA GCTGCTGCCA GGCCTGATCC ACGTGCACAG ACGGAAGATG
    TTTCAGGCCA CAGTCCTCTC AGTGGAAAAC CTGCAAAGCA GCAAGTCCAC CCGGGCCACC
    ATCCTGGTGC GCCTGGACAC TGCAGGCCAG GAGGGGCTGC AGTACCAGCC AGGGGACCAC
    ATAGGCATCT GCCCGCCCAA CCGGCCGGGC CTCGTGGAGG CGCTGCTGAG CCGCGTGGAG
    GACCCACCGC CGCCCACCGA GTCTGTGGCT GTGGAGCAGC TGGAGAAGGG CAGCCCAGGC
    GGCCCTCCTC CCAGCTGGGT GCGGGACCCG CGGCTGCCCC CGTGCACGCT GCGCCAGGCT
    CTCACCTTCT TCCTGGACAT CACCTCCCCG CCCAGCCCCC GGCTTCTCCG ACTGCTCAGC
    ACCCTGGCTG AAGAACCCAG CGAACAGCAG GAGCTCGAGA CCCTCAGTCA GGACCCCCGG
    CGCTACGAGG AGTGGAAGTG GTTCCGCTGC CCCACGCTGC TGGAGGTGCT GGAGCAGTTC
    CCGTCCGTGG CACTGCCCGC CCCACTGCTC CTCACCCAGC TGCCCCTGCT GCAGCCCCGG
    TACTACTCCG TCAGCTCGGC CCCCAGCGCC CACCCCGGAG AGGTCCACCT CACGGTGGCT
    GTGCTGGCGT ACAGGACCCA AGATGGGCTG GGCCCCCTAC ACTACGGGGT CTGCTCCACG
    TGGCTGAGCC AACTCAAGAC TGGAGACCCA GTGCCCTGCT TCATCAGGGG GGCTCCCTCT
    TTCCGGCTGC CGCCTGACCC CTACGTGCCC TGCATCCTCG TGGGCCCCGG CACTGGCATC
    GCCCCCTTCC GGGGATTTTG GCAGGAGCGG CTGCACGACA TTGAGAGCAA AGGGCTGCAG
    CCCGCCCCCA TGACCCTGGT GTTCGGCTGC CGGTGCTCCC AACTCGACCA TCTCTACCGC
    GACGAGGTGC AGGACGCCCA GGAGCGCGGG GTGTTTGGCC GCGTCCTCAC CGCCTTCTCC
    CGGGAACCTG ACAGCCCCAA GACCTACGTA CAGGACATCC TGAGAACCGA GCTGGCTGCC
    GAGGTGCACC GCGTGCTGTG CCTCGAGCGG GGCCACATGT TTGTCTGCGG CGACGTCACT
    ATGGCAACCA GCGTCCTGCA GACGGTGCAG CGCATCTTGG CGACAGAGGG CGGCATGGAG
    CTGGACGAGG CGGGCGACGT CATCGGCGTG CTGCGGGATC AGCAACGCTA TCACGAGGAC
    ATTTTTGGCC TCACGCTGCG CACCCAGGAA GTGACAAGCC GCATACGTAC CCAGAGCTTT
    TCCCTGCAGG AGCGGCATCT GCGGGGCGCG GTGCCCTGGG CCTTCGACCC GCCAGGCCCA
    GACACCCCCG GCCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027824803.1
    CDS1..3618
    Translation

    Target ORF information:

    RefSeq Version XM_027969002.1
    Organism Ovis aries(sheep)
    Definition Ovis aries nitric oxide synthase 3 (NOS3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_027969002.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAACC TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CCTGTGGCCT GGGGCTGGGG 
    CTGGGTTTTG GGCTATGCGG CAAGCAGGGC CCGGCCTCCC CAGCACCTGA GCCCAGCTGG
    GCCCCCGCAC CTGCCACCCC GCAAGCGCCA GACCACAGCC CAGCTCCCAG CAGCCCCACG
    CTGACCCGGC CTCCGGAGGG GCCCAAGTTC CCTCGCGTGA AGAACTGGGA GCTGGGGAGC
    ATCACCTACG ACACCCTGTG CGCACAGTCC CAGCAGGACG GGCCCTGCAC CCCCAGACGC
    TGCCTCGGCT CCCTGGTGTT GCCCCGGAAA CTGCAGACTC GGCCCTCCCA AGGACCTCCG
    CCCGCTGAGC AGCTGCTGAG CCAGGCCAGG GACTTCATCA ACCAGTACTA CAGCTCCATT
    AAGAGGAGCG GCTCCCAGGC TCATGAGGAG CGGCTTCAGG AGGTGGAGGC CGAGGTGGCA
    TCCACGGGCA CCTACCACCT CCGAGAGAGC GAGCTGGTGT TCGGGGCCAA GCAGGCCTGG
    CGCAACGCGC CCCGCTGCGT GGGCCGCATC CAGTGGGGGA AGCTGCAGGT GTTCGATGCC
    CGGGACTGCA GCTCAGCACA GGAGATGTTC ACCTACATCT GCAACCACAT CAAGTACGCC
    ACCAACCGTG GCAACCTTCG CTCGGCCATC ACAGTGTTCC CGCAGCGCGC CCCGGGCCGC
    GGAGACTTCC GGATCTGGAA CACCCAGCTG GTGCGCTACG CAGGCTACAG ACAGCAGGAC
    GGCTCTGTGC GTGGGGACCC GGCCAACGTG GAGATCACGG AGCTCTGCAT CCAGCACGGC
    TGGTCCCCCG GAAACGGCCG CTTCGACGTG CTGCCCCTGC TGCTCCAGGC CCCAGATGAG
    GCTCCAGAGC TCTTTGTTCT GCCCCCCGAG CTGGTCCTCG AAGTGCCCCT GGAGCACCCC
    ACACTGGAGT GGTTCGCGGC CCTGGGCCTG CGCTGGTATG CCCTCCCGGC CGTGTCCAAC
    ATGCTGCTGG AAATCGGGGG TCTGGAGTTC CCTGCGGCCC CCTTCAGCGG CTGGTACATG
    AGCACGGAGA TTGGCACACG GAACCTGTGT GACCCTCACC GCTACAACAT CCTGGAGGAT
    GTGGCTGTCT GCATGGACCT CGACACGCGG ACCACCTCGT CCCTGTGGAA GGACAAGGCG
    GCCGTGGAGA TCAACCTGGC TGTGCTGCAC AGCTTTCAGC TCGCCAAGGT GACCATCGTG
    GACCACCACG CCGCCACAGT GTCCTTCATG AAGCACCTGG AAAACGAGCA GAAGGCCCGG
    GGGGGCTGCC CCGCCGACTG GGCCTGGATC GTGCCCCCCA TCTCGGGCAG CCTCACGCCC
    GTCTTCCACC AGGAGATGGT CAATTACGTC CTGTCCCCTG CCTTCCGCTA CCAGCCAGAC
    CCCTGGAAGG GGAGCGCGGC CAAGGGCGCC GGCATCACCA GGAAGAAGAC CTTTAAGGAA
    GTGGCCAACG CGGTGAAGAT CTCTGCCTCA CTCATGGGCA CCCTGATGGC CAAGCGAGTG
    AAAGCAACCA TCCTGTACGC CTCTGAGACC GGCCGGGCCC AGAGCTATGC TCAGCAGCTG
    GGGAGGCTCT TCCGGAAGGC CTTTGACCCC CGGGTCCTGT GCATGGACGA GTATGACGTG
    GTGTCCCTGG AGCACGAGGC GCTGGTGCTG GTGGTGACCA GCACCTTTGG GAATGGCGAT
    CCCCCGGAGA ATGGAGAGAG TTTTGCAGCT GCCCTGATGG AGATGTCGGG GCCCTACAAC
    AGCTCCCCGA GGCCGGAACA GCACAAGAGT TACAAGATCC GCTTCAACAG TGTCTCCTGC
    TCCGACCCGC TGGTGTCCTC CTGGCGGCGG AAGAGAAAGG AGTCCAGCAA CACAGACAGC
    GCAGGAGCCC TGGGGACCCT CAGGTTCTGT GTGTTTGGAC TGGGCTCCCG GGCGTATCCC
    CACTTCTGCG CCTTCGCGCG TGCGGTGGAC ACCCGGCTGG AAGAGCTGGG AGGGGAGCGG
    CTGCTGCAGC TGGGCCAGGG CGACGAGCTC TGCGGCCAGG AGGAGGCCTT CCGTGGCTGG
    GCAAAGGCGG CCTTCCAGGC CTCCTGCGAG ACGTTCTGCG TCGGGGAGGA GGCCAAGGCC
    GCCGCCCAGG ACATCTTCAG CCCCAAACGG AGCTGGAAAC GCCAGAGGTA CCGGCTGAGC
    ACCCAGGCCG AGGGCCTCCA GCTGCTGCCA GGCCTGATCC ACGTGCACAG ACGGAAGATG
    TTTCAGGCCA CAGTCCTCTC AGTGGAAAAC CTGCAAAGCA GCAAGTCCAC CCGGGCCACC
    ATCCTGGTGC GCCTGGACAC TGCAGGCCAG GAGGGGCTGC AGTACCAGCC AGGGGACCAC
    ATAGGCATCT GCCCGCCCAA CCGGCCGGGC CTCGTGGAGG CGCTGCTGAG CCGCGTGGAG
    GACCCACCGC CGCCCACCGA GTCTGTGGCT GTGGAGCAGC TGGAGAAGGG CAGCCCAGGC
    GGCCCTCCTC CCAGCTGGGT GCGGGACCCG CGGCTGCCCC CGTGCACGCT GCGCCAGGCT
    CTCACCTTCT TCCTGGACAT CACCTCCCCG CCCAGCCCCC GGCTTCTCCG ACTGCTCAGC
    ACCCTGGCTG AAGAACCCAG CGAACAGCAG GAGCTCGAGA CCCTCAGTCA GGACCCCCGG
    CGCTACGAGG AGTGGAAGTG GTTCCGCTGC CCCACGCTGC TGGAGGTGCT GGAGCAGTTC
    CCGTCCGTGG CACTGCCCGC CCCACTGCTC CTCACCCAGC TGCCCCTGCT GCAGCCCCGG
    TACTACTCCG TCAGCTCGGC CCCCAGCGCC CACCCCGGAG AGGTCCACCT CACGGTGGCT
    GTGCTGGCGT ACAGGACCCA AGATGGGCTG GGCCCCCTAC ACTACGGGGT CTGCTCCACG
    TGGCTGAGCC AACTCAAGAC TGGAGACCCA GTGCCCTGCT TCATCAGGGG GGCTCCCTCT
    TTCCGGCTGC CGCCTGACCC CTACGTGCCC TGCATCCTCG TGGGCCCCGG CACTGGCATC
    GCCCCCTTCC GGGGATTTTG GCAGGAGCGG CTGCACGACA TTGAGAGCAA AGGGCTGCAG
    CCCGCCCCCA TGACCCTGGT GTTCGGCTGC CGGTGCTCCC AACTCGACCA TCTCTACCGC
    GACGAGGTGC AGGACGCCCA GGAGCGCGGG GTGTTTGGCC GCGTCCTCAC CGCCTTCTCC
    CGGGAACCTG ACAGCCCCAA GACCTACGTA CAGGACATCC TGAGAACCGA GCTGGCTGCC
    GAGGTGCACC GCGTGCTGTG CCTCGAGCGG GGCCACATGT TTGTCTGCGG CGACGTCACT
    ATGGCAACCA GCGTCCTGCA GACGGTGCAG CGCATCTTGG CGACAGAGGG CGGCATGGAG
    CTGGACGAGG CGGGCGACGT CATCGGCGTG CTGCGGGATC AGCAACGCTA TCACGAGGAC
    ATTTTTGGCC TCACGCTGCG CACCCAGGAA GTGACAAGCC GCATACGTAC CCAGAGCTTT
    TCCCTGCAGG AGCGGCATCT GCGGGGCGCG GTGCCCTGGG CCTTCGACCC GCCAGGCCCA
    GACACCCCCG GCCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm00217
    Clone ID Related Accession (Same CDS sequence) NM_001129901.1
    Accession Version NM_001129901.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-12
    Organism Ovis aries(sheep)
    Product nitric oxide synthase, endothelial
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ015701.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ015701.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00103467, SAMN00103503 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CCTGCGGCCT GGGGCTGGGG 
    CTGGGCCTCG GGCTATGCGG CAAGCAGGGC CCAGCCTCCC CGGCACCTGA GCCCAGCCGG
    GCCCCCGCAC CCGCCACCCC GCACGCGCCA GACCACAGCC CAGCTCCCAA CAGCCCCACG
    CTGACCCGGC CTCCGGAGGG GCCCAAGTTC CCTCGCGTGA AGAACTGGGA GCTGGGGAGC
    ATCACCTACG ACACTCTGTG CGCGCAGTCC CAACAGGACG GGCCCTGCAC TCCCAGGTGC
    TGCCTGGGCT CCCTGGTGTT GCCCCGGAAA CTGCAGACCC GGCCCTCCCC GGGACCTCCA
    CCCGCTGAGC AGCTGCTGAG CCAGGCCAGG GACTTCATCA ACCAGTACTA CAGCTCCATC
    AAGAGGAGTG GCTCCCAGGC TCACGAGGAG CGGCTTCAGG AGGTGGAGGC CGAGGTGGCA
    TCCACGGGCA CCTACCACCT CCGAGAGAGC GAGCTGGTGT TCGGGGCCAA GCAGGCCTGG
    CGCAACGCAC CCCGCTGCGT GGGCCGCATC CAGTGGGGGA AGCTGCAGGT GTTTGATGCC
    CGGGACTGCA GCTCAGCACA GGAGATGTTC ACCTACATCT GCAACCACAT CAAGTACGCC
    ACCAACCGCG GCAACCTTCG CTCGGCCATC ACAGTGTTCC CGCAGCGCGC CCCGGGCCGC
    GGAGACTTCC GGATCTGGAA CAGCCAGCTG GTGCGCTACG CAGGCTACAG ACAGCAGGAT
    GGCTCTGTGC GTGGGGACCC AGCCAATGTG GAGATCACGG AGCTCTGCAT CCAGCACGGC
    TGGACCCCCG GAAACGGCCG CTTCGACGTG CTGCCCCTGC TGCTCCAGGC CCCAGACGAG
    GCTCCAGAGC TCTTTGTTCT GCCCCCCGAG CTGGTCCTTG AAGTGCCCCT GGAGCACCCC
    ACACTGGAGT GGTTCGCGGC CCTGGGCCTG CGATGGTATG CCCTCCCGGC CGTGTCCAAC
    ATGCTGCTGG AAATCGGGGG TCTGGAGTTC TCCGCGGCCC CCTTCAGCGG CTGGTACATG
    AGCACGGAGA TTGGCACGCG GAACCTGTGT GACCCTCACC GCTACAATAT CCTGGAGGAT
    GTGGCCGTCT GCATGGACCT CGACACGCGG ACCACCTCGT CCCTGTGGAA GGACAAGGCG
    GCCGTGGAGA TCAACCTGGC TGTGCTGCAC AGCTTTCAGC TCGCCAAGGT GACCATCGTG
    GACCACCACG CCGCCACGGT GTCCTTCATG AAGCACCTGG ACAACGAGCA GAAGGCCAGG
    GGGGGCTGCC CCGCCGACTG GGCCTGGATC GTGCCCCCCA TCTCAGGCAG CCTCACGCCC
    GTCTTCCACC AGGAGATGGT CAACTACATC CTGTCCCCTG CCTTCCGCTA CCAGCCAGAC
    CCCTGGAAAG GGAGCGCGAC CAAGGGCGCA GGCATCACCA GGAAGAAGAC CTTTAAGGAA
    GTGGCCAACG CGGTGAAGAT CTCTGCCTCA CTCATGGGCA CCCTGATGGC CAAGCGAGTG
    AAAGCAACCA TCCTGTACGC CTCTGAGACC GGCCGGGCCC AGAGCTACGC TCAGCAGCTG
    GGGAGGCTCT TCCGGAAGGC CTTCGATCCC CGGGTCCTGT GCATGGATGA GTATGACGTG
    GTGTCCCTGG AGCACGAGGC GCTGGTACTG GTGGTGACCA GCACCTTTGG GAATGGCGAT
    CCCCCGGAGA ATGGAGAGAG TTTTGCAGCT GCCCTGATGG AGATGTCGGG GCCCTACAAC
    AGCTCCCCGC GGCCGGAACA GCACAAGAGT TACAAGATCC GCTTCAACAG CGTCTCCTGC
    TCAGACCCGC TGGTGTCCTC CTGGCGGCGG AAGAGAAAGG AGTCCAGCAA CACAGACAGT
    GCGGGGGCCC TGGGGACCCT CAGGTTCTGT GTGTTCGGAC TGGGCTCCCG GGCATACCCC
    CACTTCTGCG CCTTCGCGCG AGCGGTGGAC ACCCGGCTGG AAGAGCTTGG AGGGGAGCGG
    CTGCTGCAGC TGGGCCAGGG CGATGAGCTC TGCGGCCAGG AAGAGGCCTT CCGTGGTTGG
    GCAAAGGCGG CCTTCCAGGC CTCCTGTGAG ACCTTCTGCG TTGGGGAGGA GGCCAAGGCC
    GCCGCCCAGG ACATCTTCAG CCCCAAACGG AGCTGGAAAC GCCAGAGGTA CCGGCTGAGC
    ACCCAGGCCG AGGGCCTCCA GCTGCTGCCA GGCCTGATCC ACGTGCACAG ACGGAAGATG
    TTTCAGGCCA CAGTCCTCTC GGTGGAAAAT CTGCAAAGCA GCAAGTCCAC CCGGGCCACC
    ATCCTGGTGC GCCTGGACAC TGCAGGCCAG GAGGGGCTGC AGTACCAGCC GGGGGACCAC
    ATAGGCATCT GCCCGCCCAA CCGGCCGGGC CTGGTGGAGG CGCTGCTGAG CCGCGTGGAG
    GACCCGCCAC CGCCCACCGA GTCTGTGGCT GTGGAGCAGC TGGAGAAAGG CAGCCCAGGC
    GGCCCTCCTC CCAGCTGGGT GCGGGACCCA CGGCTGCCCC CGTGCACGCT GCGCCAGGCT
    CTCACCTTCT TCCTGGACAT CACCTCCCCA CCCAGCCCCC GGCTTCTCCG ACTGCTCAGC
    ACCCTGGCCG AAGAACCCAG CGAGCAGCAG GAGCTTGAGA CCCTCAGTCA GGACCCCCGG
    CGCTACGAGG AGTGGAAGTG GTTCCGCTGC CCCACGCTGC TGGAGGTGCT GGAGCAGTTC
    CCGTCCGTGG CGCTGCCCGC CCCGCTGCTC CTCACCCAGC TGCCCCTGCT GCAGCCCCGG
    TACTACTCTG TCAGCTCGGC CCCCAACGCC CACCCCGGAG AGGTCCACCT CACAGTGGCC
    GTGCTGGCGT ACAGGACCCA AGATGGGCTG GGCCCCCTAC ACTACGGGGT CTGCTCCACA
    TGGCTGAGCC AGCTCAAGAC TGGAGACCCC GTGCCCTGCT TCATCAGGGG GGCTCCCTCC
    TTCCGGCTGC CGCCTGACCC CTACGTGCCC TGCATCCTCG TGGGCCCTGG CACTGGCATC
    GCCCCCTTCC GGGGATTTTG GCAGGAGAGG CTGCATGACA TTGAGAGCAA AGGGCTGCAG
    CCCGCCCCCA TGACCCTGGT GTTCGGCTGC CGCTGCTCCC AACTCGACCA TCTCTACCGC
    GACGAGGTGC AGGACGCCCA GGAGCGCGGG GTGTTTGGCC GCGTCCTCAC CGCCTTCTCC
    CGGGAACCTG ACAGCCCCAA GACCTACGTA CAGGACATCC TGAGAACCGA GCTGGCTGCC
    GAGGTGCACC GCGTGCTGTG CCTCGAGCGG GGCCACATGT TTGTCTGCGG CGATGTCACT
    ATGGCAACCA GCGTCCTGCA GACGGTGCAG CGCATCTTGG CGACAGAGGG CGACATGGAG
    CTGGACGAGG CGGGCGACGT CATCGGCGTG CTGCGGGATC AGCAACGCTA TCACGAGGAC
    ATTTTCGGCC TCACGCTGCG CACCCAGGAG GTGACAAGCC GTATACGTAC CCAGAGCTTT
    TCCCTGCAGG AGCGGCATCT GCGGGGCGCG GTGCCCTGGG CCTTCGACCC GCCCGGCCCA
    GACACCCCCG GCCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001123373.1
    CDS29..3646
    Translation

    Target ORF information:

    RefSeq Version NM_001129901.1
    Organism Ovis aries(sheep)
    Definition Ovis aries nitric oxide synthase 3 (NOS3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001129901.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CCTGCGGCCT GGGGCTGGGG 
    CTGGGCCTCG GGCTATGCGG CAAGCAGGGC CCAGCCTCCC CGGCACCTGA GCCCAGCCGG
    GCCCCCGCAC CCGCCACCCC GCACGCGCCA GACCACAGCC CAGCTCCCAA CAGCCCCACG
    CTGACCCGGC CTCCGGAGGG GCCCAAGTTC CCTCGCGTGA AGAACTGGGA GCTGGGGAGC
    ATCACCTACG ACACTCTGTG CGCGCAGTCC CAACAGGACG GGCCCTGCAC TCCCAGGTGC
    TGCCTGGGCT CCCTGGTGTT GCCCCGGAAA CTGCAGACCC GGCCCTCCCC GGGACCTCCA
    CCCGCTGAGC AGCTGCTGAG CCAGGCCAGG GACTTCATCA ACCAGTACTA CAGCTCCATC
    AAGAGGAGTG GCTCCCAGGC TCACGAGGAG CGGCTTCAGG AGGTGGAGGC CGAGGTGGCA
    TCCACGGGCA CCTACCACCT CCGAGAGAGC GAGCTGGTGT TCGGGGCCAA GCAGGCCTGG
    CGCAACGCAC CCCGCTGCGT GGGCCGCATC CAGTGGGGGA AGCTGCAGGT GTTTGATGCC
    CGGGACTGCA GCTCAGCACA GGAGATGTTC ACCTACATCT GCAACCACAT CAAGTACGCC
    ACCAACCGCG GCAACCTTCG CTCGGCCATC ACAGTGTTCC CGCAGCGCGC CCCGGGCCGC
    GGAGACTTCC GGATCTGGAA CAGCCAGCTG GTGCGCTACG CAGGCTACAG ACAGCAGGAT
    GGCTCTGTGC GTGGGGACCC AGCCAATGTG GAGATCACGG AGCTCTGCAT CCAGCACGGC
    TGGACCCCCG GAAACGGCCG CTTCGACGTG CTGCCCCTGC TGCTCCAGGC CCCAGACGAG
    GCTCCAGAGC TCTTTGTTCT GCCCCCCGAG CTGGTCCTTG AAGTGCCCCT GGAGCACCCC
    ACACTGGAGT GGTTCGCGGC CCTGGGCCTG CGATGGTATG CCCTCCCGGC CGTGTCCAAC
    ATGCTGCTGG AAATCGGGGG TCTGGAGTTC TCCGCGGCCC CCTTCAGCGG CTGGTACATG
    AGCACGGAGA TTGGCACGCG GAACCTGTGT GACCCTCACC GCTACAATAT CCTGGAGGAT
    GTGGCCGTCT GCATGGACCT CGACACGCGG ACCACCTCGT CCCTGTGGAA GGACAAGGCG
    GCCGTGGAGA TCAACCTGGC TGTGCTGCAC AGCTTTCAGC TCGCCAAGGT GACCATCGTG
    GACCACCACG CCGCCACGGT GTCCTTCATG AAGCACCTGG ACAACGAGCA GAAGGCCAGG
    GGGGGCTGCC CCGCCGACTG GGCCTGGATC GTGCCCCCCA TCTCAGGCAG CCTCACGCCC
    GTCTTCCACC AGGAGATGGT CAACTACATC CTGTCCCCTG CCTTCCGCTA CCAGCCAGAC
    CCCTGGAAAG GGAGCGCGAC CAAGGGCGCA GGCATCACCA GGAAGAAGAC CTTTAAGGAA
    GTGGCCAACG CGGTGAAGAT CTCTGCCTCA CTCATGGGCA CCCTGATGGC CAAGCGAGTG
    AAAGCAACCA TCCTGTACGC CTCTGAGACC GGCCGGGCCC AGAGCTACGC TCAGCAGCTG
    GGGAGGCTCT TCCGGAAGGC CTTCGATCCC CGGGTCCTGT GCATGGATGA GTATGACGTG
    GTGTCCCTGG AGCACGAGGC GCTGGTACTG GTGGTGACCA GCACCTTTGG GAATGGCGAT
    CCCCCGGAGA ATGGAGAGAG TTTTGCAGCT GCCCTGATGG AGATGTCGGG GCCCTACAAC
    AGCTCCCCGC GGCCGGAACA GCACAAGAGT TACAAGATCC GCTTCAACAG CGTCTCCTGC
    TCAGACCCGC TGGTGTCCTC CTGGCGGCGG AAGAGAAAGG AGTCCAGCAA CACAGACAGT
    GCGGGGGCCC TGGGGACCCT CAGGTTCTGT GTGTTCGGAC TGGGCTCCCG GGCATACCCC
    CACTTCTGCG CCTTCGCGCG AGCGGTGGAC ACCCGGCTGG AAGAGCTTGG AGGGGAGCGG
    CTGCTGCAGC TGGGCCAGGG CGATGAGCTC TGCGGCCAGG AAGAGGCCTT CCGTGGTTGG
    GCAAAGGCGG CCTTCCAGGC CTCCTGTGAG ACCTTCTGCG TTGGGGAGGA GGCCAAGGCC
    GCCGCCCAGG ACATCTTCAG CCCCAAACGG AGCTGGAAAC GCCAGAGGTA CCGGCTGAGC
    ACCCAGGCCG AGGGCCTCCA GCTGCTGCCA GGCCTGATCC ACGTGCACAG ACGGAAGATG
    TTTCAGGCCA CAGTCCTCTC GGTGGAAAAT CTGCAAAGCA GCAAGTCCAC CCGGGCCACC
    ATCCTGGTGC GCCTGGACAC TGCAGGCCAG GAGGGGCTGC AGTACCAGCC GGGGGACCAC
    ATAGGCATCT GCCCGCCCAA CCGGCCGGGC CTGGTGGAGG CGCTGCTGAG CCGCGTGGAG
    GACCCGCCAC CGCCCACCGA GTCTGTGGCT GTGGAGCAGC TGGAGAAAGG CAGCCCAGGC
    GGCCCTCCTC CCAGCTGGGT GCGGGACCCA CGGCTGCCCC CGTGCACGCT GCGCCAGGCT
    CTCACCTTCT TCCTGGACAT CACCTCCCCA CCCAGCCCCC GGCTTCTCCG ACTGCTCAGC
    ACCCTGGCCG AAGAACCCAG CGAGCAGCAG GAGCTTGAGA CCCTCAGTCA GGACCCCCGG
    CGCTACGAGG AGTGGAAGTG GTTCCGCTGC CCCACGCTGC TGGAGGTGCT GGAGCAGTTC
    CCGTCCGTGG CGCTGCCCGC CCCGCTGCTC CTCACCCAGC TGCCCCTGCT GCAGCCCCGG
    TACTACTCTG TCAGCTCGGC CCCCAACGCC CACCCCGGAG AGGTCCACCT CACAGTGGCC
    GTGCTGGCGT ACAGGACCCA AGATGGGCTG GGCCCCCTAC ACTACGGGGT CTGCTCCACA
    TGGCTGAGCC AGCTCAAGAC TGGAGACCCC GTGCCCTGCT TCATCAGGGG GGCTCCCTCC
    TTCCGGCTGC CGCCTGACCC CTACGTGCCC TGCATCCTCG TGGGCCCTGG CACTGGCATC
    GCCCCCTTCC GGGGATTTTG GCAGGAGAGG CTGCATGACA TTGAGAGCAA AGGGCTGCAG
    CCCGCCCCCA TGACCCTGGT GTTCGGCTGC CGCTGCTCCC AACTCGACCA TCTCTACCGC
    GACGAGGTGC AGGACGCCCA GGAGCGCGGG GTGTTTGGCC GCGTCCTCAC CGCCTTCTCC
    CGGGAACCTG ACAGCCCCAA GACCTACGTA CAGGACATCC TGAGAACCGA GCTGGCTGCC
    GAGGTGCACC GCGTGCTGTG CCTCGAGCGG GGCCACATGT TTGTCTGCGG CGATGTCACT
    ATGGCAACCA GCGTCCTGCA GACGGTGCAG CGCATCTTGG CGACAGAGGG CGACATGGAG
    CTGGACGAGG CGGGCGACGT CATCGGCGTG CTGCGGGATC AGCAACGCTA TCACGAGGAC
    ATTTTCGGCC TCACGCTGCG CACCCAGGAG GTGACAAGCC GTATACGTAC CCAGAGCTTT
    TCCCTGCAGG AGCGGCATCT GCGGGGCGCG GTGCCCTGGG CCTTCGACCC GCCCGGCCCA
    GACACCCCCG GCCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm49322
    Clone ID Related Accession (Same CDS sequence) XM_015095442.1
    Accession Version XM_015095442.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries(sheep)
    Product LOW QUALITY PROTEIN: nitric oxide synthase, endothelial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639013.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAACC TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CCTGTGGCCT GGGGCTGGGG 
    CTGGGTTTTG GGCTATGCGG CAAGCAGGGC CCGGCCTCCC CAGCACCTGA GCCCAGCTGG
    GCCCCCGCAC CTGCCACCCC GCAAGCGCCA GACCACAGCC CAGCTCCCAG CAGCCCCACG
    CTGACCCGGC CTCCGGAGGG GCCCAAGTTC CCTCGCGTGA AGAACTGGGA GCTGGGGAGC
    ATCACCTACG ACACCCTGTG CGCACAGTCC CAGCAGGACG GGCCCTGCAC CCCCAGACGC
    TGCCTCGGCT CCCTGGTGTT GCCCCGGAAA CTGCAGACTC GGCCCTCCCA AGGACCTCCG
    CCCGCTGAGC AGCTGCTGAG CCAGGCCAGG GACTTCATCA ACCAGTACTA CAGCTCCATT
    AAGAGGAGCG GCTCCCAGGC TCATGAGGAG CGGCTTCAGG AGGTGGAGGC CGAGGTGGCA
    TCCACGGGCA CCTACCACCT CCGAGAGAGC GAGCTGGTGT TCGGGGCCAA GCAGGCCTGG
    CGCAACGCGC CCCGCTGCGT GGGCCGCATC CAGTGGGGGA AGCTGCAGGT GTTCGATGCC
    CGGGACTGCA GCTCAGCACA GGAGATGTTC ACCTACATCT GCAACCACAT CAAGTACGCC
    ACCAACCGTG GCAACCTTCG CTCGGCCATC ACAGTGTTCC CGCAGCGCAC CCCGGGCCGC
    GGAGACTTCC GGATCTGGAA CACCCAGCTG GTGCGCTACG CAGGCTACAG ACAGCAGGAC
    GGCTCTGTGC GTGGGGACCC GGCCAACGTG GAGATCACGG AGCTCTGCAT CCAGCACGGC
    TGGTCCCCCG GAAACGGCCG CTTCGACGTG CTGCCCCTGC TGCTCCAGGC CCCAGATGAG
    GCTCCAGAGC TCTTTGTTCT GCCCCCCGAG CTGGTCCTCG AAGTGCCCCT GGAGCACCCC
    ACACTGGAGT GGTTCGCGGC CCTGGGCCTG CGCTGGTATG CCCTCCCGGC CGTGTCCAAC
    ATGCTGCTGG AAATCGGGGG TCTGGAGTTC CCTGCGGCCC CCTTCAGCGG CTGGTACATG
    AGCACGGAGA TTGGCACGCG GAACCTGTGT GACCCTCACC GCTACAACAT CCTGGAGGAT
    GTGGCTGTCT GCATGGACCT CGACACGCGG ACCACCTCGT CCATGTGGAA GGACAAGGCG
    GCCGTGGAGA TCAACCTGGC TGTGCTGCAC AGCTTTCAGC CCCCCAAGGT GACCATCGTG
    GACCACCACG CCGCCACAGT GTCCTTCATG AAGCACCTGG AAAACGAGCA GAAGGCCCGG
    GGGGGCTGCC CCGCCGACTG GGCCTGGATC GTGCCCCCCA TCTCGGGCAG CCTCACGCCC
    GTCTTCCACC AGGAGATGGT CAATTACGTC CTGTCCCCTG CCTTCCGCTA CCAGCCAGAC
    CCCTGGAAGG GGAGCGCGGC CAAGGGCGCC GGCATCACCA GGAAGAAGAC CTTTAAGGAA
    GTGGCCAACG CGGTGAAGAT CTCTGCCTCA CTCATGGGCA CCCTGATGGC CAAGCGAGTG
    AAAGCAACCA TCCTGTACGC CTCTGAGACC GGCCGGGCCC AGAGCTATGC TCAGCAGCTG
    GGGAGGCTCT TCCGGAAGGC CTTTGACCCC CGGGTCCTGT GCATGGACGA GTATGACGTG
    GTGTCCCTGG AGCACGAGGC GCTGGTGCTG GTGGTGACCA GCACCTTTGG GAATGGCGAT
    CCCCCGGAGA ATGGAGAGAG TTTTGCAGCT GCCCTGATGG AGATGTCGGG GCCCTACAAC
    AGCTCCCCGA GGCCGGAACA GCACAGTGGG GGTGGGGGGA GTTACAAGAT CCGCTTCAAC
    AGTGTCTCCT GCTCCGACCC GCTGGTGTCC TCCTGGCGGC GGAAGAGAAA GGAGTCCAGC
    AACACAGACA GCGCAGGGGC CCTGGGGACC CTCAGGTTCT GTGTGTTTGG ACTGGGCTCC
    CGGGCGTATC CCCACTTCTG CGCCTTCGCG CGTGCGGTGG ACACCCGGCT GGAAGAGCTG
    GGAGGGGAGC GGCTGGGGCA GCTGGGGCAG GGGGGCGAGC TCCGCGGCCA GGGGGAGGGC
    TTCCGCGGGT GGGGGGAGGG CGCTTCCCAG GCCTCCTGCG AGACGTTCTG CGTCGGGGAG
    GAGGCCAAGG CCGCCGCCCA GGACATCTTC AGCCCCAAAC GGAGCTGGAA ACGCCAGAGG
    TACCGGCTGA GCACCCAGGC CGAGGGCCTC CAGCTGCTGC CAGGCCTGAT CCACGTGCAC
    AGACGGAAGA TGTTTCAGGC CACAGTCCTC TCAGTGGAAA ACCTGCAAAG CAGCAAGTCC
    ACCCGGGCCA CCATCCTGGT GCGCCTGGAC ACTGCAGGCC AGGAGGGGCT GCAGTACCAG
    CCAGGGGACC ACATAGGCNN NNCTCCCCCA AGAAGCAGCC ATCGCCCCGG ACAAGGCGGG
    CCCAGGGTGG CTCCATTCTC TGAGCGTCCC CTCATGCCCC GTACCCCACC CCCAGGCGGC
    CCTCCTCCCA GCTGGGTGCG GGACCCGCGG CTGCCCCCGT GCACGCTGCG CCAGGCTCTC
    ACCTTCTTCC TGGACATCAC CTCCCCGCCC AGCCCCCGGC TTCTCCGACT GCTCAGCACC
    CTGGCTGAAG AACCCAGCGA ACAGCAGGAG CTCGAGACCC TCAGTCAGGA CCCCCGGCGC
    TACGAGGAGT GGAAGTGGTT CCGCTGCCCC ACGCTGCTGG AGGTGCTGGA GCAGTTCCCG
    TCCGTGGCAC TGCCCGCCCC ACTGCTCCTC ACCCAGCTGC CCCTGCTGCA GCCCCGGTAC
    TACTCCGTCA GCTCGGCCCC CAGCGCCCAC CCCGGAGAGG TCCACCTCAC GGTGGCTGTG
    CTGGCGTACA GGACCCAAGA TGGGCTGGGC CCCCTACACT ACGGGNNTGC CTCCACGTGG
    CTGAGCCAAC TCAAGACTGG AGACCCAGTG CCCTGCTTCA TCAGGGGTGA GGCTCCCTCT
    TTCCGGCTGC CGCCTGACCC CTACGTGCCC TGCATCCTCG TGGGCCCCGG CACTGGCATC
    GCCCCCTTCC GGGGATTTTG GCAGGAGCGG CTGCACGACA TTGAGAGCAA AGGACTCCAA
    AGGGCTGCAC CCACCCTGGT GTTCGGCTGC CGGTGCTCCC AACTCGACCA TCTCTACCGC
    GACGAGGTGC AGGACGCCCA GGAGCGCGGG GTGTTTGGCC GCGTCCTCAC CGCCTTCTCC
    CGGGAACCTG ACAGCCCCAA GACCTACGTA CAGGACATCC TGAGAACCGA GCTGGCTGCC
    GAGGTGCACC GCGTGCTGTG CCTCGAGCGG GGCCACATGT TTGTCTGCGG CGACGTCACT
    ATGGCAACCA GCGTCCTGCA GACGGTGCCG CGCATCTTGG CGACAGAGGG CGGCATGGAG
    CTGGACGAGG CGGGCGACGT CATCGGCGTG CTGCGGGATC AGCAACGCTA TCACGAGGAC
    ATTTTTGGCC TCACGCTGCG CACCCAGGAA GTGACAAGCC GCATACGTAC CCAGAGCTTT
    TCCCTGCAGG AGCGGCATCT GCGGGGCGCG GTGCCCTGGG CCTTCGACCC GCCAGGCCCA
    GACACCCCCG GCCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014950928.1
    CDS43..3660
    Translation

    Target ORF information:

    RefSeq Version XM_015095442.1
    Organism Ovis aries(sheep)
    Definition Ovis aries nitric oxide synthase 3 (NOS3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015095442.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGCAACC TGAAGAGTGT GGGCCAGGAG CCCGGGCCCC CCTGTGGCCT GGGGCTGGGG 
    CTGGGTTTTG GGCTATGCGG CAAGCAGGGC CCGGCCTCCC CAGCACCTGA GCCCAGCTGG
    GCCCCCGCAC CTGCCACCCC GCAAGCGCCA GACCACAGCC CAGCTCCCAG CAGCCCCACG
    CTGACCCGGC CTCCGGAGGG GCCCAAGTTC CCTCGCGTGA AGAACTGGGA GCTGGGGAGC
    ATCACCTACG ACACCCTGTG CGCACAGTCC CAGCAGGACG GGCCCTGCAC CCCCAGACGC
    TGCCTCGGCT CCCTGGTGTT GCCCCGGAAA CTGCAGACTC GGCCCTCCCA AGGACCTCCG
    CCCGCTGAGC AGCTGCTGAG CCAGGCCAGG GACTTCATCA ACCAGTACTA CAGCTCCATT
    AAGAGGAGCG GCTCCCAGGC TCATGAGGAG CGGCTTCAGG AGGTGGAGGC CGAGGTGGCA
    TCCACGGGCA CCTACCACCT CCGAGAGAGC GAGCTGGTGT TCGGGGCCAA GCAGGCCTGG
    CGCAACGCGC CCCGCTGCGT GGGCCGCATC CAGTGGGGGA AGCTGCAGGT GTTCGATGCC
    CGGGACTGCA GCTCAGCACA GGAGATGTTC ACCTACATCT GCAACCACAT CAAGTACGCC
    ACCAACCGTG GCAACCTTCG CTCGGCCATC ACAGTGTTCC CGCAGCGCAC CCCGGGCCGC
    GGAGACTTCC GGATCTGGAA CACCCAGCTG GTGCGCTACG CAGGCTACAG ACAGCAGGAC
    GGCTCTGTGC GTGGGGACCC GGCCAACGTG GAGATCACGG AGCTCTGCAT CCAGCACGGC
    TGGTCCCCCG GAAACGGCCG CTTCGACGTG CTGCCCCTGC TGCTCCAGGC CCCAGATGAG
    GCTCCAGAGC TCTTTGTTCT GCCCCCCGAG CTGGTCCTCG AAGTGCCCCT GGAGCACCCC
    ACACTGGAGT GGTTCGCGGC CCTGGGCCTG CGCTGGTATG CCCTCCCGGC CGTGTCCAAC
    ATGCTGCTGG AAATCGGGGG TCTGGAGTTC CCTGCGGCCC CCTTCAGCGG CTGGTACATG
    AGCACGGAGA TTGGCACGCG GAACCTGTGT GACCCTCACC GCTACAACAT CCTGGAGGAT
    GTGGCTGTCT GCATGGACCT CGACACGCGG ACCACCTCGT CCATGTGGAA GGACAAGGCG
    GCCGTGGAGA TCAACCTGGC TGTGCTGCAC AGCTTTCAGC CCCCCAAGGT GACCATCGTG
    GACCACCACG CCGCCACAGT GTCCTTCATG AAGCACCTGG AAAACGAGCA GAAGGCCCGG
    GGGGGCTGCC CCGCCGACTG GGCCTGGATC GTGCCCCCCA TCTCGGGCAG CCTCACGCCC
    GTCTTCCACC AGGAGATGGT CAATTACGTC CTGTCCCCTG CCTTCCGCTA CCAGCCAGAC
    CCCTGGAAGG GGAGCGCGGC CAAGGGCGCC GGCATCACCA GGAAGAAGAC CTTTAAGGAA
    GTGGCCAACG CGGTGAAGAT CTCTGCCTCA CTCATGGGCA CCCTGATGGC CAAGCGAGTG
    AAAGCAACCA TCCTGTACGC CTCTGAGACC GGCCGGGCCC AGAGCTATGC TCAGCAGCTG
    GGGAGGCTCT TCCGGAAGGC CTTTGACCCC CGGGTCCTGT GCATGGACGA GTATGACGTG
    GTGTCCCTGG AGCACGAGGC GCTGGTGCTG GTGGTGACCA GCACCTTTGG GAATGGCGAT
    CCCCCGGAGA ATGGAGAGAG TTTTGCAGCT GCCCTGATGG AGATGTCGGG GCCCTACAAC
    AGCTCCCCGA GGCCGGAACA GCACAGTGGG GGTGGGGGGA GTTACAAGAT CCGCTTCAAC
    AGTGTCTCCT GCTCCGACCC GCTGGTGTCC TCCTGGCGGC GGAAGAGAAA GGAGTCCAGC
    AACACAGACA GCGCAGGGGC CCTGGGGACC CTCAGGTTCT GTGTGTTTGG ACTGGGCTCC
    CGGGCGTATC CCCACTTCTG CGCCTTCGCG CGTGCGGTGG ACACCCGGCT GGAAGAGCTG
    GGAGGGGAGC GGCTGGGGCA GCTGGGGCAG GGGGGCGAGC TCCGCGGCCA GGGGGAGGGC
    TTCCGCGGGT GGGGGGAGGG CGCTTCCCAG GCCTCCTGCG AGACGTTCTG CGTCGGGGAG
    GAGGCCAAGG CCGCCGCCCA GGACATCTTC AGCCCCAAAC GGAGCTGGAA ACGCCAGAGG
    TACCGGCTGA GCACCCAGGC CGAGGGCCTC CAGCTGCTGC CAGGCCTGAT CCACGTGCAC
    AGACGGAAGA TGTTTCAGGC CACAGTCCTC TCAGTGGAAA ACCTGCAAAG CAGCAAGTCC
    ACCCGGGCCA CCATCCTGGT GCGCCTGGAC ACTGCAGGCC AGGAGGGGCT GCAGTACCAG
    CCAGGGGACC ACATAGGCNN NNCTCCCCCA AGAAGCAGCC ATCGCCCCGG ACAAGGCGGG
    CCCAGGGTGG CTCCATTCTC TGAGCGTCCC CTCATGCCCC GTACCCCACC CCCAGGCGGC
    CCTCCTCCCA GCTGGGTGCG GGACCCGCGG CTGCCCCCGT GCACGCTGCG CCAGGCTCTC
    ACCTTCTTCC TGGACATCAC CTCCCCGCCC AGCCCCCGGC TTCTCCGACT GCTCAGCACC
    CTGGCTGAAG AACCCAGCGA ACAGCAGGAG CTCGAGACCC TCAGTCAGGA CCCCCGGCGC
    TACGAGGAGT GGAAGTGGTT CCGCTGCCCC ACGCTGCTGG AGGTGCTGGA GCAGTTCCCG
    TCCGTGGCAC TGCCCGCCCC ACTGCTCCTC ACCCAGCTGC CCCTGCTGCA GCCCCGGTAC
    TACTCCGTCA GCTCGGCCCC CAGCGCCCAC CCCGGAGAGG TCCACCTCAC GGTGGCTGTG
    CTGGCGTACA GGACCCAAGA TGGGCTGGGC CCCCTACACT ACGGGNNTGC CTCCACGTGG
    CTGAGCCAAC TCAAGACTGG AGACCCAGTG CCCTGCTTCA TCAGGGGTGA GGCTCCCTCT
    TTCCGGCTGC CGCCTGACCC CTACGTGCCC TGCATCCTCG TGGGCCCCGG CACTGGCATC
    GCCCCCTTCC GGGGATTTTG GCAGGAGCGG CTGCACGACA TTGAGAGCAA AGGACTCCAA
    AGGGCTGCAC CCACCCTGGT GTTCGGCTGC CGGTGCTCCC AACTCGACCA TCTCTACCGC
    GACGAGGTGC AGGACGCCCA GGAGCGCGGG GTGTTTGGCC GCGTCCTCAC CGCCTTCTCC
    CGGGAACCTG ACAGCCCCAA GACCTACGTA CAGGACATCC TGAGAACCGA GCTGGCTGCC
    GAGGTGCACC GCGTGCTGTG CCTCGAGCGG GGCCACATGT TTGTCTGCGG CGACGTCACT
    ATGGCAACCA GCGTCCTGCA GACGGTGCCG CGCATCTTGG CGACAGAGGG CGGCATGGAG
    CTGGACGAGG CGGGCGACGT CATCGGCGTG CTGCGGGATC AGCAACGCTA TCACGAGGAC
    ATTTTTGGCC TCACGCTGCG CACCCAGGAA GTGACAAGCC GCATACGTAC CCAGAGCTTT
    TCCCTGCAGG AGCGGCATCT GCGGGGCGCG GTGCCCTGGG CCTTCGACCC GCCAGGCCCA
    GACACCCCCG GCCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.